Rh4AG236900

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002436
Physical Location & Seq
Forward (+)
54178956 .. 54180276
1321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG236900.1

Sequence Viewer

Length: 891 bp
ATGGCTTCTGTACTGTCTGCTCAAGAAGCCTCACAGCTACTCAAATGCCAGACATGGATCTTGAAAGTGTTTATCCACTGTGAAGGTTGCAAGAGGAAAGTGAAGAAAGTTCTACTGGGAATTGATGGTGTTTACACCACATCTATTGAGGCAGAGCAAAACAGAGTCACAGTCACTGGAGACATTGACGTTCAGACCCTCATCAAGAAGCTGAATGTAAAAACTGGTAAACATGCTGAGCTATGGCCAGAAAATCTCACCAAGAAAGAAAAGACCAAGCCTGTGAAAGCCAAGAACAACGACAAACAGAAATCCTCAGAAATTTGCCAAAGCAAGCAGAGTACTACATGTGACAAAGTTGGAATGGTCAGGCTAAACAGAGCAAGTGAGGGTGACACAGCTAAGTTTAACAGAGAGGAACAATGTCAAGTATCAAAGAAGGGTCACATTCCGCCAGAGAAATCTCCTGATGGCCATGATTCTCAGGCCATGGATCTTCAGCTCGGTGAAAGTGAAAAAGGGAGTGTTAGTGGCAAAAAGAACAGGAAAGGCCAAAGAGATGATTTGGGTTCAACAAGCTCCTGTGGACCTGCAAGCATAGGATCTCAACATTATGGTGATCACACTGCTGGTCCAGTTGATCTCAGCCATATCATCGCAAACATGTATCCGATGGATTACCACTCTCATCTGGTGTTCAATGGAAGTGACATTACAGCAGCTGCTACAGCACTGCCTAGCAAGAGCTTGGCTCCTTACTATTGTTATGGTCCATCATCACAGTACAAGGATGCAGCTAGTGGTGAACAGATTGATCAAGTGAAAGCAACATTTCTTAACAGTGTGCAGATTTTCAGTGATGAAAATGCTAATGGCTGTTTTATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

296

Amino Acids

32.3

Weight (kDa)

8.45

Isoelectric Point (pI)

35.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 22 - 71 1.7e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 598
AciI CCGC 1 cut(s) 452
AclWI GGATC 3 cut(s) 65, 501, 610
AcoI YGGCCR 2 cut(s) 245, 472
AcsI RAATTY 1 cut(s) 321
AcuI CTGAAG 1 cut(s) 482
AfaI GTAC 3 cut(s) 12, 343, 785
AflIII ACRYGT 2 cut(s) 347, 663
AgsI TTSAA 3 cut(s) 64, 573, 700
AluBI AGCT 9 cut(s) 37, 211, 241, 401, 502, 579, 722, 747, 797
AluI AGCT 9 cut(s) 37, 211, 241, 401, 502, 579, 722, 747, 797
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 3 cut(s) 65, 501, 610
AlwNI CAGNNNCTG 2 cut(s) 176, 722
AoxI GGCC 4 cut(s) 245, 472, 486, 550
ApeKI GCWGC 3 cut(s) 719, 722, 794
ApoI RAATTY 1 cut(s) 321
AspS9I GGNCC 3 cut(s) 587, 632, 770
AsuHPI GGTGA 5 cut(s) 250, 404, 518, 629, 815
AvaII GGWCC 3 cut(s) 587, 632, 770
BalI TGGCCA 2 cut(s) 247, 474
BarI GAAGNNNNNNTAC 2 cut(s) 697, 729
BbvI GCAGC 3 cut(s) 709, 731, 806
BccI CCATC 4 cut(s) 119, 464, 667, 781
BciVI GTATCC 1 cut(s) 678
BclI TGATCA 2 cut(s) 619, 814
BcoDI GTCTC 1 cut(s) 174
BfaI CTAG 2 cut(s) 738, 798
BfmI CTRYAG 1 cut(s) 726
BfuAI ACCTGC 1 cut(s) 598
BfuI GTATCC 1 cut(s) 678
BisI GCNGC 3 cut(s) 720, 723, 795
BlpI GCTNAGC 1 cut(s) 237
BlsI GCNGC 3 cut(s) 721, 724, 796
BmcAI AGTACT 1 cut(s) 343
Bme18I GGWCC 3 cut(s) 587, 632, 770
BmgT120I GGNCC 3 cut(s) 587, 632, 770
BmiI GGNNCC 1 cut(s) 753
BmrI ACTGGG 1 cut(s) 125
BmsI GCATC 1 cut(s) 781
BmuI ACTGGG 1 cut(s) 125
BplI GAGNNNNNCTC 2 cut(s) 736, 768
BpmI CTGGAG 1 cut(s) 198
Bpu1102I GCTNAGC 1 cut(s) 237
BpuEI CTTGAG 1 cut(s) 6
BsaJI CCNNGG 1 cut(s) 489
BsaXI ACNNNNNCTCC 2 cut(s) 514, 544
Bse1I ACTGG 4 cut(s) 120, 181, 229, 635
BseDI CCNNGG 1 cut(s) 489
BseGI GGATG 1 cut(s) 796
BseMII CTCAG 4 cut(s) 228, 330, 497, 658
BseNI ACTGG 4 cut(s) 120, 181, 229, 635
BseXI GCAGC 3 cut(s) 709, 731, 806
BsgI GTGCAG 1 cut(s) 866
BshFI GGCC 4 cut(s) 247, 474, 488, 552
BsmAI GTCTC 1 cut(s) 174
BsnI GGCC 4 cut(s) 247, 474, 488, 552
Bsp143I GATC 6 cut(s) 57, 493, 602, 619, 640, 814
Bsp1720I GCTNAGC 1 cut(s) 237
Bsp19I CCATGG 1 cut(s) 489
BspACI CCGC 1 cut(s) 452
BspANI GGCC 4 cut(s) 247, 474, 488, 552
BspCNI CTCAG 4 cut(s) 229, 329, 496, 657
BspLI GGNNCC 1 cut(s) 753
BspMI ACCTGC 1 cut(s) 598
BspPI GGATC 3 cut(s) 65, 501, 610
BsrI ACTGG 4 cut(s) 120, 181, 229, 635
BssECI CCNNGG 1 cut(s) 489
BssMI GATC 6 cut(s) 57, 493, 602, 619, 640, 814
BssT1I CCWWGG 1 cut(s) 489
Bst4CI ACNGT 5 cut(s) 15, 80, 172, 783, 842
BstC8I GCNNGC 2 cut(s) 335, 595
BstDEI CTNAG 5 cut(s) 237, 316, 402, 483, 644
BstDSI CCRYGG 1 cut(s) 489
BstF5I GGATG 1 cut(s) 796
BstKTI GATC 6 cut(s) 60, 496, 605, 622, 643, 817
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 6 cut(s) 57, 493, 602, 619, 640, 814
BstMWI GCNNNNNNNGC 2 cut(s) 26, 728
BstNSI RCATGY 3 cut(s) 236, 351, 667
BstSFI CTRYAG 1 cut(s) 726
BstV1I GCAGC 3 cut(s) 709, 731, 806
BstX2I RGATCY 3 cut(s) 57, 493, 602
BstYI RGATCY 3 cut(s) 57, 493, 602
BsuI GTATCC 1 cut(s) 678
BsuRI GGCC 4 cut(s) 247, 474, 488, 552
BtgI CCRYGG 1 cut(s) 489
BtgZI GCGATG 1 cut(s) 640
BtsCI GGATG 1 cut(s) 796
BtsI GCAGTG 2 cut(s) 624, 731
BtsIMutI CAGTG 6 cut(s) 76, 174, 624, 731, 847, 862
BveI ACCTGC 1 cut(s) 598
Cac8I GCNNGC 2 cut(s) 335, 595
CaiI CAGNNNCTG 2 cut(s) 176, 722
Cfr13I GGNCC 3 cut(s) 587, 632, 770
Csp6I GTAC 3 cut(s) 11, 342, 784
CspCI CAANNNNNGTGG 2 cut(s) 127, 162
CviAII CATG 6 cut(s) 54, 233, 348, 476, 490, 664
CviQI GTAC 3 cut(s) 11, 342, 784
DdeI CTNAG 5 cut(s) 237, 316, 402, 483, 644
DpnI GATC 6 cut(s) 59, 495, 604, 621, 642, 816
DpnII GATC 6 cut(s) 57, 493, 602, 619, 640, 814
EaeI YGGCCR 2 cut(s) 245, 472
EciI GGCGGA 1 cut(s) 441
Eco130I CCWWGG 1 cut(s) 489
Eco47I GGWCC 3 cut(s) 587, 632, 770
Eco57I CTGAAG 1 cut(s) 482
EcoT14I CCWWGG 1 cut(s) 489
ErhI CCWWGG 1 cut(s) 489
FaeI CATG 6 cut(s) 57, 236, 351, 479, 493, 667
FatI CATG 6 cut(s) 53, 232, 347, 475, 489, 663
FbaI TGATCA 2 cut(s) 619, 814
Fnu4HI GCNGC 3 cut(s) 720, 723, 795
FokI GGATG 1 cut(s) 803
Fsp4HI GCNGC 3 cut(s) 720, 723, 795
FspBI CTAG 2 cut(s) 738, 798
GluI GCNGC 3 cut(s) 720, 723, 795
GsuI CTGGAG 1 cut(s) 198
HaeIII GGCC 4 cut(s) 247, 474, 488, 552
Hin1II CATG 6 cut(s) 57, 236, 351, 479, 493, 667
HinfI GANTC 2 cut(s) 165, 479
HphI GGTGA 5 cut(s) 250, 404, 518, 629, 815
Hpy166II GTNNAC 4 cut(s) 133, 230, 587, 806
Hpy188I TCNGA 3 cut(s) 195, 319, 672
Hpy188III TCNNGA 4 cut(s) 23, 61, 205, 467
Hpy8I GTNNAC 4 cut(s) 133, 230, 587, 806
HpyAV CCTTC 2 cut(s) 77, 433
HpyCH4III ACNGT 5 cut(s) 15, 80, 172, 783, 842
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 4 cut(s) 90, 593, 794, 847
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 728
HpyF3I CTNAG 5 cut(s) 237, 316, 402, 483, 644
HpySE526I ACGT 1 cut(s) 189
Hsp92II CATG 6 cut(s) 57, 236, 351, 479, 493, 667
Ksp22I TGATCA 2 cut(s) 619, 814
Kzo9I GATC 6 cut(s) 57, 493, 602, 619, 640, 814
LmnI GCTCC 2 cut(s) 584, 757
Lsp1109I GCAGC 3 cut(s) 709, 731, 806
LweI GCATC 1 cut(s) 781
MaeI CTAG 2 cut(s) 738, 798
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 6 cut(s) 166, 172, 350, 392, 443, 707
MalI GATC 6 cut(s) 59, 495, 604, 621, 642, 816
MboI GATC 6 cut(s) 57, 493, 602, 619, 640, 814
MboII GAAGA 2 cut(s) 115, 488
MflI RGATCY 3 cut(s) 57, 493, 602
MlsI TGGCCA 2 cut(s) 247, 474
MluCI AATT 2 cut(s) 120, 321
MluNI TGGCCA 2 cut(s) 247, 474
MlyI GAGTC 1 cut(s) 174
MmeI TCCRAC 1 cut(s) 340
MnlI CCTC 7 cut(s) 40, 87, 142, 209, 325, 382, 409
Mox20I TGGCCA 2 cut(s) 247, 474
MscI TGGCCA 2 cut(s) 247, 474
MseI TTAA 2 cut(s) 408, 837
MslI CAYNNNNRTG 1 cut(s) 615
Msp20I TGGCCA 2 cut(s) 247, 474
MspA1I CMGCKG 1 cut(s) 722
MwoI GCNNNNNNNGC 2 cut(s) 26, 728
NcoI CCATGG 1 cut(s) 489
NdeII GATC 6 cut(s) 57, 493, 602, 619, 640, 814
NlaIII CATG 6 cut(s) 57, 236, 351, 479, 493, 667
NlaIV GGNNCC 1 cut(s) 753
NmuCI GTSAC 6 cut(s) 166, 172, 350, 392, 443, 707
NspI RCATGY 3 cut(s) 236, 351, 667
PciI ACATGT 2 cut(s) 347, 663
PfeI GAWTC 1 cut(s) 479
PkrI GCNGC 3 cut(s) 721, 724, 796
PleI GAGTC 1 cut(s) 173
PpsI GAGTC 1 cut(s) 173
PscI ACATGT 2 cut(s) 347, 663
PspN4I GGNNCC 1 cut(s) 753
PspPI GGNCC 3 cut(s) 587, 632, 770
PstNI CAGNNNCTG 2 cut(s) 176, 722
PsuI RGATCY 3 cut(s) 57, 493, 602
PvuII CAGCTG 1 cut(s) 722
RsaI GTAC 3 cut(s) 12, 343, 785
RsaNI GTAC 3 cut(s) 11, 342, 784
RseI CAYNNNNRTG 1 cut(s) 615
SaqAI TTAA 2 cut(s) 408, 837
SatI GCNGC 3 cut(s) 720, 723, 795
Sau3AI GATC 6 cut(s) 57, 493, 602, 619, 640, 814
Sau96I GGNCC 3 cut(s) 587, 632, 770
ScaI AGTACT 1 cut(s) 343
SchI GAGTC 1 cut(s) 174
SfaNI GCATC 1 cut(s) 781
SfcI CTRYAG 1 cut(s) 726
SinI GGWCC 3 cut(s) 587, 632, 770
SmiMI CAYNNNNRTG 1 cut(s) 615
SmlI CTYRAG 1 cut(s) 21
SmoI CTYRAG 1 cut(s) 21
Sse9I AATT 2 cut(s) 120, 321
SsiI CCGC 1 cut(s) 452
SspMI CTAG 2 cut(s) 738, 798
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 5 cut(s) 15, 80, 172, 783, 842
TaiI ACGT 1 cut(s) 192
TasI AATT 2 cut(s) 120, 321
TatI WGTACW 3 cut(s) 10, 341, 783
TfiI GAWTC 1 cut(s) 479
Tru1I TTAA 2 cut(s) 408, 837
Tru9I TTAA 2 cut(s) 408, 837
TscAI CASTG 6 cut(s) 83, 181, 631, 738, 847, 862
TseFI GTSAC 6 cut(s) 166, 172, 350, 392, 443, 707
TseI GCWGC 3 cut(s) 719, 722, 794
Tsp45I GTSAC 6 cut(s) 166, 172, 350, 392, 443, 707
TspDTI ATGAA 1 cut(s) 876
TspRI CASTG 6 cut(s) 83, 181, 631, 738, 847, 862
VpaK11BI GGWCC 3 cut(s) 587, 632, 770
XapI RAATTY 1 cut(s) 321
XceI RCATGY 3 cut(s) 236, 351, 667
XspI CTAG 2 cut(s) 738, 798
ZrmI AGTACT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.