FvH4_4g32260
ERF Family

heavy metal transport detoxification superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004807
Physical Location & Seq
Forward (+)
30982322 .. 30984562
2241 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g32260.t1

Sequence Viewer

Length: 1245 bp
ATGACTAAAGATGAAGACTTTAAGCTCCTCAAGATCCAGACTTGTTATCTCAAAGTGAACATACACTGTGATGGGTGTAAGCAGAAAGTGAAGAAACTGCTTCAGAGAATTGAAGGTGTTTATGAAGTCAGCATAGATGCTGATCAGCAGAAAGTCACAGTTTCAGGGGTTATAGACTCTGCCAATTTGATAAAGAAATTGGTTAGAGCTGGGAAACATGCTGAGCTTTGGAACCAGCAGAAGGCCAATCAGAACCAAAACCAGAAGCAAAAGAACAACATCTGCATCAAGGACAACAAAGGCCAAAAGCAGCAAGGGGGCATGATAAAGGGACTTGAAGCCTTCAAAAACCAGCAGCCGCAGCAGAAGTTTCCCCATTTCTTCACTGAAGAGGATATTAATGACTTATTGGAAGATGAGGAGGAGGATGAGAAGGATGAGTTGAGGCTTCTGATGAGGCAGAAAGTAAACCAGCTCAGGCATCAATCTATTGATGTAAACAACAATGGAAGGAAAGGTGCTGCTGCTGCAGCTAACCCAAACAATAGTGTCAAGATCACAAACAATCACAATGCTGGGAAGAAGGGAGTGCAGCACCAAAATCAGAATGTGCAGGGCATGAAGGTGATTAATCCAGGTGGGATTGATCCCAAAACCATGGCAGCACTGAACATGAGCAACATTGCTCAATTGGGTGCCCAATTGGGTGGCGAAGCCAAGAGGGGGAATAACAATGACTTGAGCACCATGATGAATTTGGCTGGTTTTCATGGCAATGGCGCTACAAATGCTGCTTCTGCCGCCGGTTTAGGTGGCAATGTGGCCCATGCCAATAGTCTAGCAGCATTCCAACAAGCGCTTCAATCTCAGCAATCTCAAGCTGGAGGCAGTGCATATCAGGCTCAATCAGCTTCTGGAGGGTTTCCTAGTGGAGGTTATGCCACTGGCCAATATCCGCAGACTATGATGATGAATACAAATGGCTACGGGCATCCATCACAGATGATGAACATGAATATGCAGCAGGCGAGGCAACAAGCAATACAGCAGCAGCAACCTCAGATGATGTATCACAGGTCTCCATACATTCCTCCTAGTACTACTGGTTACTACCCCAACTATGGTCCCATTCCTAGTTCTGCTCCATATCCTTACACTGCCGAGCCTAATTACGGTGGTGATGATGGCAACAATGCAGCTCATATGTTCAATGATGAGAACACCAGCAGCTGTTCTATAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

415

Amino Acids

45.27

Weight (kDa)

8.74

Isoelectric Point (pI)

40.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 18 - 73 1.5e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 695
AciI CCGC 3 cut(s) 359, 801, 956
AclWI GGATC 2 cut(s) 28, 641
AcoI YGGCCR 1 cut(s) 946
AcsI RAATTY 1 cut(s) 754
AcuI CTGAAG 2 cut(s) 86, 408
AfaI GTAC 1 cut(s) 1099
AfeI AGCGCT 1 cut(s) 858
AfiI CCNNNNNNNGG 5 cut(s) 241, 723, 932, 1121, 1172
AgsI TTSAA 5 cut(s) 113, 338, 346, 863, 1210
AjnI CCWGG 1 cut(s) 634
AjuI GAANNNNNNNTTGG 2 cut(s) 843, 875
AluBI AGCT 9 cut(s) 25, 209, 226, 475, 533, 881, 911, 1199, 1230
AluI AGCT 9 cut(s) 25, 209, 226, 475, 533, 881, 911, 1199, 1230
Alw21I GWGCWC 1 cut(s) 746
Alw26I GTCTC 1 cut(s) 1083
AlwI GGATC 2 cut(s) 28, 641
AlwNI CAGNNNCTG 2 cut(s) 914, 1230
Aor51HI AGCGCT 1 cut(s) 858
AoxI GGCC 4 cut(s) 243, 301, 822, 946
ApoI RAATTY 1 cut(s) 754
AseI ATTAAT 2 cut(s) 399, 630
AspLEI GCGC 2 cut(s) 782, 859
AspS9I GGNCC 2 cut(s) 823, 1124
AsuHPI GGTGA 2 cut(s) 637, 1190
AvaII GGWCC 1 cut(s) 1124
BaeGI GKGCMC 1 cut(s) 700
BalI TGGCCA 1 cut(s) 948
BanI GGYRCC 1 cut(s) 695
BbsI GAAGAC 1 cut(s) 21
Bbv12I GWGCWC 1 cut(s) 746
BccI CCATC 3 cut(s) 65, 1003, 1178
BciT130I CCWGG 1 cut(s) 636
BclI TGATCA 1 cut(s) 142
BcoDI GTCTC 1 cut(s) 1083
BfaI CTAG 4 cut(s) 839, 927, 1095, 1134
BfmI CTRYAG 1 cut(s) 528
BfoI RGCGCY 2 cut(s) 783, 860
BlpI GCTNAGC 1 cut(s) 222
BmcAI AGTACT 1 cut(s) 1099
Bme1390I CCNGG 1 cut(s) 636
Bme18I GGWCC 1 cut(s) 1124
BmgT120I GGNCC 2 cut(s) 823, 1124
BmiI GGNNCC 3 cut(s) 233, 697, 1126
BmrFI CCNGG 1 cut(s) 636
BmsI GCATC 4 cut(s) 127, 294, 490, 1000
BpiI GAAGAC 1 cut(s) 21
BpmI CTGGAG 2 cut(s) 903, 936
Bpu10I CCTNAGC 1 cut(s) 476
Bpu1102I GCTNAGC 1 cut(s) 222
BpuEI CTTGAG 3 cut(s) 14, 760, 861
BsaBI GATNNNNATC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 1083
BsaJI CCNNGG 1 cut(s) 657
Bsc4I CCNNNNNNNGG 5 cut(s) 241, 723, 932, 1121, 1172
Bse118I RCCGGY 1 cut(s) 803
Bse1I ACTGG 2 cut(s) 949, 1108
Bse3DI GCAATG 3 cut(s) 681, 781, 823
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 1 cut(s) 636
BseDI CCNNGG 1 cut(s) 657
BseGI GGATG 3 cut(s) 433, 442, 991
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 5 cut(s) 241, 723, 932, 1121, 1172
BseMI GCAATG 3 cut(s) 681, 781, 823
BseMII CTCAG 4 cut(s) 213, 490, 881, 1073
BseNI ACTGG 2 cut(s) 949, 1108
BseRI GAGGAG 3 cut(s) 17, 434, 437
BseSI GKGCMC 1 cut(s) 700
BseYI CCCAGC 2 cut(s) 209, 575
BsgI GTGCAG 2 cut(s) 611, 632
BshFI GGCC 4 cut(s) 245, 303, 824, 948
BshNI GGYRCC 1 cut(s) 695
BsiHKAI GWGCWC 1 cut(s) 746
BsiSI CCGG 1 cut(s) 804
BslFI GGGAC 2 cut(s) 345, 1110
BslI CCNNNNNNNGG 5 cut(s) 241, 723, 932, 1121, 1172
BsmAI GTCTC 1 cut(s) 1083
BsmFI GGGAC 2 cut(s) 345, 1110
BsmI GAATGC 1 cut(s) 845
BsnI GGCC 4 cut(s) 245, 303, 824, 948
Bso31I GGTCTC 1 cut(s) 1083
Bsp1286I GDGCHC 2 cut(s) 700, 746
Bsp143I GATC 4 cut(s) 33, 142, 555, 646
Bsp1720I GCTNAGC 1 cut(s) 222
Bsp19I CCATGG 1 cut(s) 657
BspACI CCGC 3 cut(s) 359, 801, 956
BspANI GGCC 4 cut(s) 245, 303, 824, 948
BspCNI CTCAG 4 cut(s) 214, 489, 880, 1072
BspLI GGNNCC 3 cut(s) 233, 697, 1126
BspMAI CTGCAG 1 cut(s) 532
BspPI GGATC 2 cut(s) 28, 641
BspT107I GGYRCC 1 cut(s) 695
BspTNI GGTCTC 1 cut(s) 1083
BsrDI GCAATG 3 cut(s) 681, 781, 823
BsrFI RCCGGY 1 cut(s) 803
BsrI ACTGG 2 cut(s) 949, 1108
BssAI RCCGGY 1 cut(s) 803
BssECI CCNNGG 1 cut(s) 657
BssMI GATC 4 cut(s) 33, 142, 555, 646
BssT1I CCWWGG 1 cut(s) 657
Bst2UI CCWGG 1 cut(s) 636
Bst4CI ACNGT 3 cut(s) 68, 160, 1175
Bst6I CTCTTC 1 cut(s) 384
BstC8I GCNNGC 1 cut(s) 1026
BstDEI CTNAG 4 cut(s) 222, 476, 867, 1059
BstDSI CCRYGG 1 cut(s) 657
BstENI CCTNNNNNAGG 1 cut(s) 930
BstF5I GGATG 3 cut(s) 433, 442, 991
BstH2I RGCGCY 2 cut(s) 783, 860
BstHHI GCGC 2 cut(s) 782, 859
BstKTI GATC 4 cut(s) 36, 145, 558, 649
BstMAI GTCTC 1 cut(s) 1083
BstMBI GATC 4 cut(s) 33, 142, 555, 646
BstMWI GCNNNNNNNGC 9 cut(s) 361, 527, 530, 788, 797, 800, 899, 908, 1030
BstNI CCWGG 1 cut(s) 636
BstNSI RCATGY 1 cut(s) 221
BstSCI CCNGG 1 cut(s) 634
BstSFI CTRYAG 1 cut(s) 528
BstSLI GKGCMC 1 cut(s) 700
BstV2I GAAGAC 1 cut(s) 21
BstX2I RGATCY 1 cut(s) 33
BstXI CCANNNNNNTGG 2 cut(s) 658, 707
BstYI RGATCY 1 cut(s) 33
BsuRI GGCC 4 cut(s) 245, 303, 824, 948
BtgI CCRYGG 1 cut(s) 657
BtsCI GGATG 3 cut(s) 433, 442, 991
BtsI GCAGTG 2 cut(s) 895, 1155
BtsIMutI CAGTG 6 cut(s) 64, 384, 665, 895, 942, 1155
Cac8I GCNNGC 1 cut(s) 1026
CaiI CAGNNNCTG 2 cut(s) 914, 1230
CfoI GCGC 2 cut(s) 782, 859
Cfr10I RCCGGY 1 cut(s) 803
Cfr13I GGNCC 2 cut(s) 823, 1124
Csp6I GTAC 1 cut(s) 1098
CviAII CATG 9 cut(s) 218, 322, 619, 658, 673, 748, 770, 827, 1012
CviQI GTAC 1 cut(s) 1098
DdeI CTNAG 4 cut(s) 222, 476, 867, 1059
DpnI GATC 4 cut(s) 35, 144, 557, 648
DpnII GATC 4 cut(s) 33, 142, 555, 646
EaeI YGGCCR 1 cut(s) 946
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
Eco130I CCWWGG 1 cut(s) 657
Eco31I GGTCTC 1 cut(s) 1083
Eco47I GGWCC 1 cut(s) 1124
Eco47III AGCGCT 1 cut(s) 858
Eco57I CTGAAG 2 cut(s) 86, 408
EcoNI CCTNNNNNAGG 1 cut(s) 930
EcoRII CCWGG 1 cut(s) 634
EcoT14I CCWWGG 1 cut(s) 657
ErhI CCWWGG 1 cut(s) 657
FaeI CATG 9 cut(s) 221, 325, 622, 661, 676, 751, 773, 830, 1015
FaqI GGGAC 2 cut(s) 345, 1110
FatI CATG 9 cut(s) 217, 321, 618, 657, 672, 747, 769, 826, 1011
FauNDI CATATG 1 cut(s) 1203
FbaI TGATCA 1 cut(s) 142
FokI GGATG 3 cut(s) 440, 449, 978
FspBI CTAG 4 cut(s) 839, 927, 1095, 1134
GlaI GCGC 2 cut(s) 781, 858
GsaI CCCAGC 2 cut(s) 213, 579
GsuI CTGGAG 2 cut(s) 903, 936
HaeII RGCGCY 2 cut(s) 783, 860
HaeIII GGCC 4 cut(s) 245, 303, 824, 948
HapII CCGG 1 cut(s) 804
HhaI GCGC 2 cut(s) 782, 859
Hin1II CATG 9 cut(s) 221, 325, 622, 661, 676, 751, 773, 830, 1015
Hin6I GCGC 2 cut(s) 780, 857
HinP1I GCGC 2 cut(s) 780, 857
HinfI GANTC 1 cut(s) 176
HpaII CCGG 1 cut(s) 804
HphI GGTGA 2 cut(s) 637, 1190
Hpy166II GTNNAC 3 cut(s) 58, 469, 499
Hpy188I TCNGA 5 cut(s) 105, 252, 453, 606, 1062
Hpy188III TCNNGA 4 cut(s) 31, 37, 553, 915
Hpy8I GTNNAC 3 cut(s) 58, 469, 499
HpyAV CCTTC 7 cut(s) 107, 235, 352, 427, 504, 577, 616
HpyCH4III ACNGT 3 cut(s) 68, 160, 1175
HpyCH4V TGCA 7 cut(s) 285, 530, 592, 613, 893, 1021, 1196
HpyF10VI GCNNNNNNNGC 9 cut(s) 361, 527, 530, 788, 797, 800, 899, 908, 1030
HpyF3I CTNAG 4 cut(s) 222, 476, 867, 1059
Hsp92II CATG 9 cut(s) 221, 325, 622, 661, 676, 751, 773, 830, 1015
HspAI GCGC 2 cut(s) 780, 857
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 4 cut(s) 33, 142, 555, 646
LmnI GCTCC 2 cut(s) 30, 1147
LweI GCATC 4 cut(s) 127, 294, 490, 1000
MaeI CTAG 4 cut(s) 839, 927, 1095, 1134
MaeIII GTNAC 2 cut(s) 154, 1106
MalI GATC 4 cut(s) 35, 144, 557, 648
MboI GATC 4 cut(s) 33, 142, 555, 646
MboII GAAGA 6 cut(s) 26, 103, 373, 401, 425, 592
MfeI CAATTG 2 cut(s) 689, 701
MflI RGATCY 1 cut(s) 33
MhlI GDGCHC 2 cut(s) 700, 746
MlsI TGGCCA 1 cut(s) 948
MluCI AATT 7 cut(s) 108, 184, 197, 689, 701, 754, 1168
MluNI TGGCCA 1 cut(s) 948
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 1 cut(s) 874
Mox20I TGGCCA 1 cut(s) 948
MscI TGGCCA 1 cut(s) 948
MseI TTAA 3 cut(s) 21, 399, 630
MslI CAYNNNNRTG 5 cut(s) 69, 623, 749, 774, 1016
Msp20I TGGCCA 1 cut(s) 948
MspA1I CMGCKG 1 cut(s) 1230
MspI CCGG 1 cut(s) 804
MspR9I CCNGG 1 cut(s) 636
MunI CAATTG 2 cut(s) 689, 701
Mva1269I GAATGC 1 cut(s) 845
MvaI CCWGG 1 cut(s) 636
MwoI GCNNNNNNNGC 9 cut(s) 361, 527, 530, 788, 797, 800, 899, 908, 1030
NcoI CCATGG 1 cut(s) 657
NdeI CATATG 1 cut(s) 1203
NdeII GATC 4 cut(s) 33, 142, 555, 646
NlaIII CATG 9 cut(s) 221, 325, 622, 661, 676, 751, 773, 830, 1015
NlaIV GGNNCC 3 cut(s) 233, 697, 1126
NmeAIII GCCGAG 1 cut(s) 1186
NmuCI GTSAC 1 cut(s) 154
NspI RCATGY 1 cut(s) 221
PctI GAATGC 1 cut(s) 845
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
PshBI ATTAAT 2 cut(s) 399, 630
Psp6I CCWGG 1 cut(s) 634
PspFI CCCAGC 2 cut(s) 209, 575
PspGI CCWGG 1 cut(s) 634
PspN4I GGNNCC 3 cut(s) 233, 697, 1126
PspPI GGNCC 2 cut(s) 823, 1124
PstI CTGCAG 1 cut(s) 532
PstNI CAGNNNCTG 2 cut(s) 914, 1230
PsuI RGATCY 1 cut(s) 33
PvuII CAGCTG 1 cut(s) 1230
RsaI GTAC 1 cut(s) 1099
RsaNI GTAC 1 cut(s) 1098
RseI CAYNNNNRTG 5 cut(s) 69, 623, 749, 774, 1016
SaqAI TTAA 3 cut(s) 21, 399, 630
Sau3AI GATC 4 cut(s) 33, 142, 555, 646
Sau96I GGNCC 2 cut(s) 823, 1124
ScaI AGTACT 1 cut(s) 1099
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 1 cut(s) 636
SduI GDGCHC 2 cut(s) 700, 746
SfaNI GCATC 4 cut(s) 127, 294, 490, 1000
SfcI CTRYAG 1 cut(s) 528
SinI GGWCC 1 cut(s) 1124
SmiMI CAYNNNNRTG 5 cut(s) 69, 623, 749, 774, 1016
SmlI CTYRAG 3 cut(s) 29, 739, 876
SmoI CTYRAG 3 cut(s) 29, 739, 876
Sse9I AATT 7 cut(s) 108, 184, 197, 689, 701, 754, 1168
SsiI CCGC 3 cut(s) 359, 801, 956
SspMI CTAG 4 cut(s) 839, 927, 1095, 1134
StyD4I CCNGG 1 cut(s) 634
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 3 cut(s) 68, 160, 1175
TasI AATT 7 cut(s) 108, 184, 197, 689, 701, 754, 1168
TatI WGTACW 1 cut(s) 1097
TauI GCSGC 2 cut(s) 361, 803
Tru1I TTAA 3 cut(s) 21, 399, 630
Tru9I TTAA 3 cut(s) 21, 399, 630
TscAI CASTG 6 cut(s) 71, 391, 672, 895, 949, 1162
TseFI GTSAC 1 cut(s) 154
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 8 cut(s) 27, 138, 635, 758, 767, 986, 1022, 1028
TspRI CASTG 6 cut(s) 71, 391, 672, 895, 949, 1162
VpaK11BI GGWCC 1 cut(s) 1124
VspI ATTAAT 2 cut(s) 399, 630
XagI CCTNNNNNAGG 1 cut(s) 930
XapI RAATTY 1 cut(s) 754
XceI RCATGY 1 cut(s) 221
XcmI CCANNNNNNNNNTGG 1 cut(s) 754
XspI CTAG 4 cut(s) 839, 927, 1095, 1134
ZrmI AGTACT 1 cut(s) 1099
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.