RLG00000010192
ERF Family

heavy metal transport detoxification superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004807
Physical Location & Seq
Reverse (-)
63996143 .. 63996638
496 bp
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UTR
Exon/CDS
Intron
RLM00000010192

Sequence Viewer

Length: 453 bp
ATGACTAAAGATGAAGACTTTAAACTCCTCGAGATCCAGACTTGTGTTCTCAAAGTGAACACACACTTTGATGGGTGTAAGCAGGAAGTAAAGAAACTGCTTCAGAGAATTGAAGGTGTTTACCAAGTCAGCATAGATGCTGATCAGCAGAAAGTCACAGTTTCAGGGGTTATAGACTCTGCCACTTTGATCAAGAAATTGGTTAGAGCTGGGACACATGCTGAGCTTTGGTCCCAGCAGAAGGCCAATCAGAAACAAAACCAGAAGCAAAAGAATACCAACTGCATCAAGGACAACAAATGCCAAAAGCAGCAGGGGCAGAAGTTCCCCAATTTCCTTACTGAAGAGGATATTGATGACTTCTTGGACGACGATGAGGAGGAGGATGAAGAGGATAAGTTGAGGTTTCTGAGGCAGAAAATGAACCAGCGACTGATGCAAACAACAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

151

Amino Acids

17.55

Weight (kDa)

5.49

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 18 - 71 3.4e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 28
AcuI CTGAAG 2 cut(s) 86, 363
AfiI CCNNNNNNNGG 1 cut(s) 241
AgsI TTSAA 1 cut(s) 113
AluBI AGCT 2 cut(s) 209, 226
AluI AGCT 2 cut(s) 209, 226
AlwI GGATC 1 cut(s) 28
AlwNI CAGNNNCTG 1 cut(s) 433
Ama87I CYCGRG 1 cut(s) 29
AoxI GGCC 1 cut(s) 243
ApeKI GCWGC 1 cut(s) 310
AspS9I GGNCC 1 cut(s) 231
AvaI CYCGRG 1 cut(s) 29
AvaII GGWCC 1 cut(s) 231
BbsI GAAGAC 1 cut(s) 21
BbvI GCAGC 1 cut(s) 322
BccI CCATC 1 cut(s) 65
BclI TGATCA 2 cut(s) 142, 189
BisI GCNGC 1 cut(s) 311
BlpI GCTNAGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 312
Bme18I GGWCC 1 cut(s) 231
BmeT110I CYCGRG 1 cut(s) 29
BmgT120I GGNCC 1 cut(s) 231
BmiI GGNNCC 1 cut(s) 233
BmsI GCATC 3 cut(s) 127, 294, 426
BpiI GAAGAC 1 cut(s) 21
Bpu1102I GCTNAGC 1 cut(s) 222
BsaBI GATNNNNATC 1 cut(s) 141
Bsc4I CCNNNNNNNGG 1 cut(s) 241
Bse8I GATNNNNATC 1 cut(s) 141
BseGI GGATG 1 cut(s) 391
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 1 cut(s) 241
BseMII CTCAG 2 cut(s) 213, 401
BseRI GAGGAG 3 cut(s) 17, 392, 395
BseXI GCAGC 1 cut(s) 322
BseYI CCCAGC 2 cut(s) 209, 234
BshFI GGCC 1 cut(s) 245
BsiHKCI CYCGRG 1 cut(s) 29
BslFI GGGAC 2 cut(s) 217, 226
BslI CCNNNNNNNGG 1 cut(s) 241
BsmFI GGGAC 2 cut(s) 217, 226
BsnI GGCC 1 cut(s) 245
BsoBI CYCGRG 1 cut(s) 29
Bsp143I GATC 3 cut(s) 33, 142, 189
Bsp1720I GCTNAGC 1 cut(s) 222
BspANI GGCC 1 cut(s) 245
BspCNI CTCAG 2 cut(s) 214, 402
BspLI GGNNCC 1 cut(s) 233
BspPI GGATC 1 cut(s) 28
BssMI GATC 3 cut(s) 33, 142, 189
Bst4CI ACNGT 1 cut(s) 160
Bst6I CTCTTC 2 cut(s) 339, 384
BstDEI CTNAG 2 cut(s) 222, 410
BstF5I GGATG 1 cut(s) 391
BstKTI GATC 3 cut(s) 36, 145, 192
BstMBI GATC 3 cut(s) 33, 142, 189
BstMWI GCNNNNNNNGC 2 cut(s) 316, 436
BstNSI RCATGY 1 cut(s) 221
BstV1I GCAGC 1 cut(s) 322
BstV2I GAAGAC 1 cut(s) 21
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
BsuRI GGCC 1 cut(s) 245
BtsCI GGATG 1 cut(s) 391
CaiI CAGNNNCTG 1 cut(s) 433
Cfr13I GGNCC 1 cut(s) 231
CviAII CATG 1 cut(s) 218
CviJI RGCY 3 cut(s) 209, 226, 245
CviKI_1 RGCY 3 cut(s) 209, 226, 245
DdeI CTNAG 2 cut(s) 222, 410
DpnI GATC 3 cut(s) 35, 144, 191
DpnII GATC 3 cut(s) 33, 142, 189
DraI TTTAAA 1 cut(s) 22
Eam1104I CTCTTC 2 cut(s) 339, 384
EarI CTCTTC 2 cut(s) 339, 384
Eco47I GGWCC 1 cut(s) 231
Eco57I CTGAAG 2 cut(s) 86, 363
Eco88I CYCGRG 1 cut(s) 29
FaeI CATG 1 cut(s) 221
FaiI YATR 3 cut(s) 134, 173, 219
FaqI GGGAC 2 cut(s) 217, 226
FatI CATG 1 cut(s) 217
FbaI TGATCA 2 cut(s) 142, 189
Fnu4HI GCNGC 1 cut(s) 311
FokI GGATG 1 cut(s) 398
Fsp4HI GCNGC 1 cut(s) 311
GluI GCNGC 1 cut(s) 311
GsaI CCCAGC 2 cut(s) 213, 238
HaeIII GGCC 1 cut(s) 245
Hin1II CATG 1 cut(s) 221
HinfI GANTC 1 cut(s) 176
Hpy166II GTNNAC 2 cut(s) 58, 121
Hpy188I TCNGA 3 cut(s) 105, 252, 411
Hpy188III TCNNGA 3 cut(s) 31, 37, 193
Hpy8I GTNNAC 2 cut(s) 58, 121
Hpy99I CGWCG 1 cut(s) 374
HpyAV CCTTC 2 cut(s) 107, 235
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4V TGCA 2 cut(s) 285, 439
HpyF10VI GCNNNNNNNGC 2 cut(s) 316, 436
HpyF3I CTNAG 2 cut(s) 222, 410
Hsp92II CATG 1 cut(s) 221
Ksp22I TGATCA 2 cut(s) 142, 189
Kzo9I GATC 3 cut(s) 33, 142, 189
LpnPI CCDG 8 cut(s) 50, 68, 150, 195, 248, 275, 299, 440
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 3 cut(s) 127, 294, 426
MaeIII GTNAC 1 cut(s) 154
MalI GATC 3 cut(s) 35, 144, 191
MboI GATC 3 cut(s) 33, 142, 189
MboII GAAGA 3 cut(s) 26, 356, 401
MflI RGATCY 1 cut(s) 33
MluCI AATT 3 cut(s) 108, 197, 331
MlyI GAGTC 1 cut(s) 170
MnlI CCTC 8 cut(s) 38, 340, 370, 373, 376, 385, 396, 405
MseI TTAA 1 cut(s) 21
MslI CAYNNNNRTG 1 cut(s) 69
MwoI GCNNNNNNNGC 2 cut(s) 316, 436
NdeII GATC 3 cut(s) 33, 142, 189
NlaIII CATG 1 cut(s) 221
NlaIV GGNNCC 1 cut(s) 233
NmuCI GTSAC 1 cut(s) 154
NspI RCATGY 1 cut(s) 221
PaeR7I CTCGAG 1 cut(s) 29
PkrI GCNGC 1 cut(s) 312
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
PspFI CCCAGC 2 cut(s) 209, 234
PspN4I GGNNCC 1 cut(s) 233
PspPI GGNCC 1 cut(s) 231
PstNI CAGNNNCTG 1 cut(s) 433
PsuI RGATCY 1 cut(s) 33
RseI CAYNNNNRTG 1 cut(s) 69
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 1 cut(s) 311
Sau3AI GATC 3 cut(s) 33, 142, 189
Sau96I GGNCC 1 cut(s) 231
SchI GAGTC 1 cut(s) 170
SetI ASST 4 cut(s) 118, 211, 228, 407
SfaNI GCATC 3 cut(s) 127, 294, 426
Sfr274I CTCGAG 1 cut(s) 29
SinI GGWCC 1 cut(s) 231
SlaI CTCGAG 1 cut(s) 29
SmiMI CAYNNNNRTG 1 cut(s) 69
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 3 cut(s) 108, 197, 331
TaaI ACNGT 1 cut(s) 160
TaqI TCGA 1 cut(s) 30
TasI AATT 3 cut(s) 108, 197, 331
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 1 cut(s) 310
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 3 cut(s) 27, 402, 437
VpaK11BI GGWCC 1 cut(s) 231
XceI RCATGY 1 cut(s) 221
XhoI CTCGAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.