Rh4AG008900
ERF Family

heavy metal transport detoxification superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004807
Physical Location & Seq
Forward (+)
1494672 .. 1495028
357 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG008900.1

Sequence Viewer

Length: 357 bp
ATGACTAAAGATGAAGACTTTAAACTCCTCGAGATCCAGACTTATGTTCTCAAAGTGAACATACACTTTGATGGGTGTAAGCAGGAAGTAAAGAAACTGCTTCAGAGAATTGAAGGTGTTTACCAAGTCAGCATAGATGCTGATCAGCAGAAAGTCACAGTTTCAGGGGTTATAGACTCTGCCACTTCGATCAAGAAATTGGTTAGAGCTGGGAAACATGCTGAGCTTTGGTCCCAGCAGAAGACCAATCAGAACCAAAACCAGAAGCAAAGGAACACCAACTGCATCAAGGATAACAAAGGCCAAAAGCAGCAGGTGTTCCCCAATTTCCTTACTGAAGAGGATATTGATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

118

Amino Acids

13.66

Weight (kDa)

8.87

Isoelectric Point (pI)

32.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 17 - 70 1.2e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 304
Acc36I ACCTGC 1 cut(s) 304
AclWI GGATC 1 cut(s) 28
AcuI CTGAAG 2 cut(s) 86, 357
AgsI TTSAA 1 cut(s) 113
AluBI AGCT 2 cut(s) 209, 226
AluI AGCT 2 cut(s) 209, 226
AlwI GGATC 1 cut(s) 28
Ama87I CYCGRG 1 cut(s) 29
AoxI GGCC 1 cut(s) 301
ApeKI GCWGC 1 cut(s) 310
AspS9I GGNCC 1 cut(s) 231
AvaI CYCGRG 1 cut(s) 29
AvaII GGWCC 1 cut(s) 231
BbsI GAAGAC 2 cut(s) 21, 248
BbvI GCAGC 1 cut(s) 322
BccI CCATC 1 cut(s) 65
BclI TGATCA 1 cut(s) 142
BfuAI ACCTGC 1 cut(s) 304
BisI GCNGC 1 cut(s) 311
BlpI GCTNAGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 312
Bme18I GGWCC 1 cut(s) 231
BmeT110I CYCGRG 1 cut(s) 29
BmgT120I GGNCC 1 cut(s) 231
BmiI GGNNCC 1 cut(s) 233
BmsI GCATC 2 cut(s) 127, 294
BpiI GAAGAC 2 cut(s) 21, 248
Bpu1102I GCTNAGC 1 cut(s) 222
Bsa29I ATCGAT 1 cut(s) 352
BsaBI GATNNNNATC 2 cut(s) 141, 348
Bse8I GATNNNNATC 2 cut(s) 141, 348
BseCI ATCGAT 1 cut(s) 352
BseJI GATNNNNATC 2 cut(s) 141, 348
BseMII CTCAG 1 cut(s) 213
BseRI GAGGAG 1 cut(s) 17
BseXI GCAGC 1 cut(s) 322
BseYI CCCAGC 2 cut(s) 209, 234
BshFI GGCC 1 cut(s) 303
BshVI ATCGAT 1 cut(s) 352
BsiHKCI CYCGRG 1 cut(s) 29
BslFI GGGAC 1 cut(s) 217
BsmFI GGGAC 1 cut(s) 217
BsnI GGCC 1 cut(s) 303
BsoBI CYCGRG 1 cut(s) 29
Bsp143I GATC 4 cut(s) 33, 142, 189, 349
Bsp1720I GCTNAGC 1 cut(s) 222
BspANI GGCC 1 cut(s) 303
BspCNI CTCAG 1 cut(s) 214
BspDI ATCGAT 1 cut(s) 352
BspLI GGNNCC 1 cut(s) 233
BspMI ACCTGC 1 cut(s) 304
BspPI GGATC 1 cut(s) 28
BssMI GATC 4 cut(s) 33, 142, 189, 349
Bst4CI ACNGT 1 cut(s) 160
Bst6I CTCTTC 1 cut(s) 333
BstDEI CTNAG 1 cut(s) 222
BstKTI GATC 4 cut(s) 36, 145, 192, 352
BstMBI GATC 4 cut(s) 33, 142, 189, 349
BstNSI RCATGY 1 cut(s) 221
BstV1I GCAGC 1 cut(s) 322
BstV2I GAAGAC 2 cut(s) 21, 248
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
Bsu15I ATCGAT 1 cut(s) 352
BsuRI GGCC 1 cut(s) 303
BsuTUI ATCGAT 1 cut(s) 352
BveI ACCTGC 1 cut(s) 304
Cfr13I GGNCC 1 cut(s) 231
ClaI ATCGAT 1 cut(s) 352
CviAII CATG 1 cut(s) 218
CviJI RGCY 3 cut(s) 209, 226, 303
CviKI_1 RGCY 3 cut(s) 209, 226, 303
DdeI CTNAG 1 cut(s) 222
DpnI GATC 4 cut(s) 35, 144, 191, 351
DpnII GATC 4 cut(s) 33, 142, 189, 349
DraI TTTAAA 1 cut(s) 22
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
Eco47I GGWCC 1 cut(s) 231
Eco57I CTGAAG 2 cut(s) 86, 357
Eco88I CYCGRG 1 cut(s) 29
FaeI CATG 1 cut(s) 221
FaiI YATR 5 cut(s) 45, 62, 134, 173, 219
FaqI GGGAC 1 cut(s) 217
FatI CATG 1 cut(s) 217
FbaI TGATCA 1 cut(s) 142
Fnu4HI GCNGC 1 cut(s) 311
Fsp4HI GCNGC 1 cut(s) 311
GluI GCNGC 1 cut(s) 311
GsaI CCCAGC 2 cut(s) 213, 238
HaeIII GGCC 1 cut(s) 303
Hin1II CATG 1 cut(s) 221
HinfI GANTC 1 cut(s) 176
Hpy166II GTNNAC 2 cut(s) 58, 121
Hpy188I TCNGA 2 cut(s) 105, 252
Hpy188III TCNNGA 3 cut(s) 31, 37, 193
Hpy8I GTNNAC 2 cut(s) 58, 121
HpyAV CCTTC 1 cut(s) 107
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4V TGCA 1 cut(s) 285
HpyF3I CTNAG 1 cut(s) 222
Hsp92II CATG 1 cut(s) 221
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 4 cut(s) 33, 142, 189, 349
LpnPI CCDG 7 cut(s) 50, 68, 150, 195, 248, 275, 299
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 2 cut(s) 127, 294
MaeIII GTNAC 1 cut(s) 154
MalI GATC 4 cut(s) 35, 144, 191, 351
MboI GATC 4 cut(s) 33, 142, 189, 349
MboII GAAGA 3 cut(s) 26, 253, 350
MflI RGATCY 1 cut(s) 33
MluCI AATT 3 cut(s) 108, 197, 325
MlyI GAGTC 1 cut(s) 170
MnlI CCTC 2 cut(s) 38, 334
MseI TTAA 1 cut(s) 21
MslI CAYNNNNRTG 1 cut(s) 69
NdeII GATC 4 cut(s) 33, 142, 189, 349
NlaIII CATG 1 cut(s) 221
NlaIV GGNNCC 1 cut(s) 233
NmuCI GTSAC 1 cut(s) 154
NspI RCATGY 1 cut(s) 221
PaeR7I CTCGAG 1 cut(s) 29
PaqCI CACCTGC 1 cut(s) 304
PkrI GCNGC 1 cut(s) 312
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
PspFI CCCAGC 2 cut(s) 209, 234
PspN4I GGNNCC 1 cut(s) 233
PspPI GGNCC 1 cut(s) 231
PsuI RGATCY 1 cut(s) 33
RseI CAYNNNNRTG 1 cut(s) 69
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 1 cut(s) 311
Sau3AI GATC 4 cut(s) 33, 142, 189, 349
Sau96I GGNCC 1 cut(s) 231
SchI GAGTC 1 cut(s) 170
SetI ASST 4 cut(s) 118, 211, 228, 318
SfaNI GCATC 2 cut(s) 127, 294
Sfr274I CTCGAG 1 cut(s) 29
SinI GGWCC 1 cut(s) 231
SlaI CTCGAG 1 cut(s) 29
SmiMI CAYNNNNRTG 1 cut(s) 69
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 3 cut(s) 108, 197, 325
TaaI ACNGT 1 cut(s) 160
TaqI TCGA 3 cut(s) 30, 188, 352
TasI AATT 3 cut(s) 108, 197, 325
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 1 cut(s) 310
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 1 cut(s) 27
VpaK11BI GGWCC 1 cut(s) 231
XceI RCATGY 1 cut(s) 221
XhoI CTCGAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.