RchiOBHm_Chr4g0441051
ERF Family

heavy metal transport detoxification superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004807
Physical Location & Seq
Forward (+)
62980733 .. 62983727
2995 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40896

Sequence Viewer

Length: 1245 bp
ATGACTAAAGATGAAGACTTTAAGCTTCTCAAGATCCAGACTTGTGTTCTCAAAGTGAACATACACTGTGATGGGTGTAAGCAGAAAGTGAAGAAACTGCTTCAGAGAATTGAAGGTGTTTACCAAGTCAGCATTGATGCTGATCAGCAGAAAGTAACAATTTCAGGGGTTATAGACGCTGCCACTTTGATCAAGAAATTGGTTAGAGCTGGGAAACATGCTGAGCTTTGGTCCCAGCAGAAGGCCAATCAGAGCCAAAACCAGAAGCAAAAGAACACCAACTGCATCAAGGACAACAAAGGCCAAAAGCAGCAGGGGGGGATAATAAAGGGCCTTGAAGCCTTCAAAAACCAGCAGCAGCAGCAGAAGTTCCCCAATTTCCTTATTGAAGAGGATATTGATGACTTCTTGGACGACGATGAGGAGGAGGATGAAGATGATGAGTTGAGGTTTCTGAGGCAGAAAGTGAACCAGCTCAGGCATCAAGCGATTGATGCAAACAACAATGCAAAGAAAGGTGTTGCGGCTGCAGCTAATCCCAACAATAGTGTCAAGATCACAAACAATCACAATGCTGGAAAGAAGGGAGTGCAGCACCCAAATCAGAATGTGCAGGGCATGAAGGTGATTAATCCAGGCGGGATTGATCCCAAAACCATGGCAGCACTGAACCTGGGCAACATTGCTCAATTGGGTGGTGGAAACATCAATTTGGGTGGGGAAGTCAAGAGGGGGAATAACCATGACCTCAGTGCCATGATGAATTTGGCTGGCTTTCATGGCAATGGTGCTGCAAATGCTGCTACTTCTGCTGCTGGTATGGGTGGCAATCCCAATGTTAGTCTAGCAGCATTGCAACAAGCGCTTCAATCTCAGCAAGCTCAAGCTGCAGGCAGTGGATATCAGGCTCAAGCAGCTTCTGGAGGGTTCCCTAGTGGTGGTTATGCGACGGGCCAATATCCGCAGACCATGATGATGAACACAAATGGCTACGGGCATCCATCGCAGATGATGAACATGAATATGCAGGCGAGGCAAGCAATGCAGCAGCAGCAACCTCAGATGATGTATCATAGGTCTCCATTCAGTCCTCCTAGTACTACTGGTTATTACCACAACTATGGTCCTACTCCTAGTTCTGTTCCATATCCATACAGTGCTGAGCCTAATTACAGCAACGGTGATGGCAACAATGCAGCTCATATGTTCAATGATGAGAACACCAGCAGCTGTTCTATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

414

Amino Acids

44.91

Weight (kDa)

8.41

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 17 - 73 1.2e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 524, 639, 962
AclWI GGATC 2 cut(s) 28, 641
AcsI RAATTY 1 cut(s) 763
AcuI CTGAAG 1 cut(s) 86
AfaI GTAC 1 cut(s) 1099
AfeI AGCGCT 1 cut(s) 864
AfiI CCNNNNNNNGG 2 cut(s) 241, 938
AgsI TTSAA 6 cut(s) 113, 338, 346, 389, 869, 1210
AjnI CCWGG 2 cut(s) 634, 672
Alw26I GTCTC 1 cut(s) 1083
AlwI GGATC 2 cut(s) 28, 641
AlwNI CAGNNNCTG 2 cut(s) 920, 1230
Aor51HI AGCGCT 1 cut(s) 864
AoxI GGCC 4 cut(s) 243, 301, 331, 952
ApoI RAATTY 1 cut(s) 763
AseI ATTAAT 1 cut(s) 630
AspLEI GCGC 1 cut(s) 865
AspS9I GGNCC 4 cut(s) 231, 331, 952, 1124
AsuHPI GGTGA 2 cut(s) 637, 1193
AvaII GGWCC 2 cut(s) 231, 1124
BbsI GAAGAC 1 cut(s) 21
BccI CCATC 3 cut(s) 65, 1009, 1178
BciT130I CCWGG 2 cut(s) 636, 674
BclI TGATCA 2 cut(s) 142, 189
BcoDI GTCTC 1 cut(s) 1083
BfaI CTAG 4 cut(s) 845, 933, 1095, 1134
BfmI CTRYAG 2 cut(s) 528, 888
BfoI RGCGCY 1 cut(s) 866
BlpI GCTNAGC 2 cut(s) 222, 1161
BmcAI AGTACT 1 cut(s) 1099
Bme1390I CCNGG 2 cut(s) 636, 674
Bme18I GGWCC 2 cut(s) 231, 1124
BmgT120I GGNCC 4 cut(s) 231, 331, 952, 1124
BmiI GGNNCC 2 cut(s) 233, 929
BmrFI CCNGG 2 cut(s) 636, 674
BmsI GCATC 5 cut(s) 127, 294, 484, 490, 1006
BpiI GAAGAC 1 cut(s) 21
BpmI CTGGAG 1 cut(s) 942
Bpu10I CCTNAGC 1 cut(s) 476
Bpu1102I GCTNAGC 2 cut(s) 222, 1161
BpuEI CTTGAG 3 cut(s) 14, 867, 894
BsaBI GATNNNNATC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 1083
BsaJI CCNNGG 2 cut(s) 657, 673
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 938
Bse1I ACTGG 1 cut(s) 1108
Bse3DI GCAATG 4 cut(s) 681, 790, 851, 1047
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 2 cut(s) 636, 674
BseDI CCNNGG 2 cut(s) 657, 673
BseGI GGATG 2 cut(s) 436, 997
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 2 cut(s) 241, 938
BseMI GCAATG 4 cut(s) 681, 790, 851, 1047
BseMII CTCAG 7 cut(s) 213, 446, 490, 763, 887, 1073, 1152
BseNI ACTGG 1 cut(s) 1108
BseRI GAGGAG 2 cut(s) 437, 440
BseYI CCCAGC 2 cut(s) 209, 234
BsgI GTGCAG 2 cut(s) 611, 632
BshFI GGCC 4 cut(s) 245, 303, 333, 954
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 2 cut(s) 241, 938
BsmAI GTCTC 1 cut(s) 1083
BsmFI GGGAC 1 cut(s) 217
BsnI GGCC 4 cut(s) 245, 303, 333, 954
Bso31I GGTCTC 1 cut(s) 1083
Bsp143I GATC 5 cut(s) 33, 142, 189, 555, 646
Bsp1720I GCTNAGC 2 cut(s) 222, 1161
Bsp19I CCATGG 1 cut(s) 657
BspACI CCGC 3 cut(s) 524, 639, 962
BspANI GGCC 4 cut(s) 245, 303, 333, 954
BspCNI CTCAG 7 cut(s) 214, 447, 489, 762, 886, 1072, 1153
BspLI GGNNCC 2 cut(s) 233, 929
BspMAI CTGCAG 2 cut(s) 532, 892
BspPI GGATC 2 cut(s) 28, 641
BspTNI GGTCTC 1 cut(s) 1083
BsrDI GCAATG 4 cut(s) 681, 790, 851, 1047
BsrI ACTGG 1 cut(s) 1108
BssECI CCNNGG 2 cut(s) 657, 673
BssMI GATC 5 cut(s) 33, 142, 189, 555, 646
BssT1I CCWWGG 1 cut(s) 657
Bst2UI CCWGG 2 cut(s) 636, 674
Bst4CI ACNGT 3 cut(s) 68, 1157, 1181
Bst6I CTCTTC 1 cut(s) 384
BstAPI GCANNNNNTGC 2 cut(s) 800, 1042
BstC8I GCNNGC 5 cut(s) 772, 879, 892, 1029, 1038
BstDEI CTNAG 7 cut(s) 222, 455, 476, 749, 873, 1059, 1161
BstDSI CCRYGG 1 cut(s) 657
BstF5I GGATG 2 cut(s) 436, 997
BstH2I RGCGCY 1 cut(s) 866
BstHHI GCGC 1 cut(s) 865
BstKTI GATC 5 cut(s) 36, 145, 192, 558, 649
BstMAI GTCTC 1 cut(s) 1083
BstMBI GATC 5 cut(s) 33, 142, 189, 555, 646
BstNI CCWGG 2 cut(s) 636, 674
BstNSI RCATGY 1 cut(s) 221
BstSCI CCNGG 2 cut(s) 634, 672
BstSFI CTRYAG 2 cut(s) 528, 888
BstV2I GAAGAC 1 cut(s) 21
BstX2I RGATCY 1 cut(s) 33
BstXI CCANNNNNNTGG 2 cut(s) 658, 1121
BstYI RGATCY 1 cut(s) 33
BsuRI GGCC 4 cut(s) 245, 303, 333, 954
BtgI CCRYGG 1 cut(s) 657
BtgZI GCGATG 1 cut(s) 987
BtsCI GGATG 2 cut(s) 436, 997
BtsI GCAGTG 1 cut(s) 901
BtsIMutI CAGTG 5 cut(s) 64, 665, 757, 901, 1162
Cac8I GCNNGC 5 cut(s) 772, 879, 892, 1029, 1038
CaiI CAGNNNCTG 2 cut(s) 920, 1230
CfoI GCGC 1 cut(s) 865
Cfr13I GGNCC 4 cut(s) 231, 331, 952, 1124
CseI GACGC 1 cut(s) 185
Csp6I GTAC 1 cut(s) 1098
CspCI CAANNNNNGTGG 2 cut(s) 697, 732
CviAII CATG 9 cut(s) 218, 619, 658, 743, 757, 779, 970, 1018, 1240
CviQI GTAC 1 cut(s) 1098
DdeI CTNAG 7 cut(s) 222, 455, 476, 749, 873, 1059, 1161
DpnI GATC 5 cut(s) 35, 144, 191, 557, 648
DpnII GATC 5 cut(s) 33, 142, 189, 555, 646
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
Eco130I CCWWGG 1 cut(s) 657
Eco31I GGTCTC 1 cut(s) 1083
Eco32I GATATC 1 cut(s) 902
Eco47I GGWCC 2 cut(s) 231, 1124
Eco47III AGCGCT 1 cut(s) 864
Eco57I CTGAAG 1 cut(s) 86
EcoO109I RGGNCCY 1 cut(s) 331
EcoRII CCWGG 2 cut(s) 634, 672
EcoRV GATATC 1 cut(s) 902
EcoT14I CCWWGG 1 cut(s) 657
ErhI CCWWGG 1 cut(s) 657
FaeI CATG 9 cut(s) 221, 622, 661, 746, 760, 782, 973, 1021, 1243
FaqI GGGAC 1 cut(s) 217
FatI CATG 9 cut(s) 217, 618, 657, 742, 756, 778, 969, 1017, 1239
FauI CCCGC 1 cut(s) 632
FauNDI CATATG 1 cut(s) 1203
FbaI TGATCA 2 cut(s) 142, 189
FokI GGATG 2 cut(s) 443, 984
FspBI CTAG 4 cut(s) 845, 933, 1095, 1134
GlaI GCGC 1 cut(s) 864
GsaI CCCAGC 2 cut(s) 213, 238
GsuI CTGGAG 1 cut(s) 942
HaeII RGCGCY 1 cut(s) 866
HaeIII GGCC 4 cut(s) 245, 303, 333, 954
HgaI GACGC 1 cut(s) 185
HhaI GCGC 1 cut(s) 865
Hin1II CATG 9 cut(s) 221, 622, 661, 746, 760, 782, 973, 1021, 1243
Hin6I GCGC 1 cut(s) 863
HinP1I GCGC 1 cut(s) 863
HindIII AAGCTT 1 cut(s) 23
HphI GGTGA 2 cut(s) 637, 1193
Hpy166II GTNNAC 3 cut(s) 58, 121, 469
Hpy188I TCNGA 5 cut(s) 105, 252, 456, 606, 1062
Hpy188III TCNNGA 6 cut(s) 31, 37, 193, 553, 727, 921
Hpy8I GTNNAC 3 cut(s) 58, 121, 469
Hpy99I CGWCG 2 cut(s) 419, 952
HpyAV CCTTC 5 cut(s) 107, 235, 352, 577, 616
HpyCH4III ACNGT 3 cut(s) 68, 1157, 1181
HpyF3I CTNAG 7 cut(s) 222, 455, 476, 749, 873, 1059, 1161
Hsp92II CATG 9 cut(s) 221, 622, 661, 746, 760, 782, 973, 1021, 1243
HspAI GCGC 1 cut(s) 863
Ksp22I TGATCA 2 cut(s) 142, 189
Kzo9I GATC 5 cut(s) 33, 142, 189, 555, 646
LweI GCATC 5 cut(s) 127, 294, 484, 490, 1006
MaeI CTAG 4 cut(s) 845, 933, 1095, 1134
MaeIII GTNAC 1 cut(s) 154
MalI GATC 5 cut(s) 35, 144, 191, 557, 648
MboI GATC 5 cut(s) 33, 142, 189, 555, 646
MboII GAAGA 4 cut(s) 26, 103, 401, 446
MfeI CAATTG 1 cut(s) 689
MflI RGATCY 1 cut(s) 33
MluCI AATT 8 cut(s) 108, 159, 197, 376, 689, 709, 763, 1168
MseI TTAA 2 cut(s) 21, 630
MslI CAYNNNNRTG 6 cut(s) 69, 623, 783, 974, 1022, 1119
MspA1I CMGCKG 1 cut(s) 1230
MspR9I CCNGG 2 cut(s) 636, 674
MunI CAATTG 1 cut(s) 689
MvaI CCWGG 2 cut(s) 636, 674
NcoI CCATGG 1 cut(s) 657
NdeI CATATG 1 cut(s) 1203
NdeII GATC 5 cut(s) 33, 142, 189, 555, 646
NlaIII CATG 9 cut(s) 221, 622, 661, 746, 760, 782, 973, 1021, 1243
NlaIV GGNNCC 2 cut(s) 233, 929
NspI RCATGY 1 cut(s) 221
PshBI ATTAAT 1 cut(s) 630
Psp6I CCWGG 2 cut(s) 634, 672
PspFI CCCAGC 2 cut(s) 209, 234
PspGI CCWGG 2 cut(s) 634, 672
PspN4I GGNNCC 2 cut(s) 233, 929
PspPI GGNCC 4 cut(s) 231, 331, 952, 1124
PstI CTGCAG 2 cut(s) 532, 892
PstNI CAGNNNCTG 2 cut(s) 920, 1230
PsuI RGATCY 1 cut(s) 33
PvuII CAGCTG 1 cut(s) 1230
RsaI GTAC 1 cut(s) 1099
RsaNI GTAC 1 cut(s) 1098
RseI CAYNNNNRTG 6 cut(s) 69, 623, 783, 974, 1022, 1119
SaqAI TTAA 2 cut(s) 21, 630
Sau3AI GATC 5 cut(s) 33, 142, 189, 555, 646
Sau96I GGNCC 4 cut(s) 231, 331, 952, 1124
ScaI AGTACT 1 cut(s) 1099
ScrFI CCNGG 2 cut(s) 636, 674
SfaNI GCATC 5 cut(s) 127, 294, 484, 490, 1006
SfcI CTRYAG 2 cut(s) 528, 888
SinI GGWCC 2 cut(s) 231, 1124
SmiMI CAYNNNNRTG 6 cut(s) 69, 623, 783, 974, 1022, 1119
SmlI CTYRAG 3 cut(s) 29, 882, 909
SmoI CTYRAG 3 cut(s) 29, 882, 909
Sse9I AATT 8 cut(s) 108, 159, 197, 376, 689, 709, 763, 1168
SsiI CCGC 3 cut(s) 524, 639, 962
SspMI CTAG 4 cut(s) 845, 933, 1095, 1134
StyD4I CCNGG 2 cut(s) 634, 672
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 3 cut(s) 68, 1157, 1181
TasI AATT 8 cut(s) 108, 159, 197, 376, 689, 709, 763, 1168
TatI WGTACW 1 cut(s) 1097
TauI GCSGC 1 cut(s) 527
Tru1I TTAA 2 cut(s) 21, 630
Tru9I TTAA 2 cut(s) 21, 630
TscAI CASTG 5 cut(s) 71, 672, 757, 901, 1162
TspDTI ATGAA 8 cut(s) 27, 447, 635, 767, 776, 992, 1028, 1034
TspRI CASTG 5 cut(s) 71, 672, 757, 901, 1162
VpaK11BI GGWCC 2 cut(s) 231, 1124
VspI ATTAAT 1 cut(s) 630
XapI RAATTY 1 cut(s) 763
XceI RCATGY 1 cut(s) 221
XcmI CCANNNNNNNNNTGG 1 cut(s) 763
XspI CTAG 4 cut(s) 845, 933, 1095, 1134
ZrmI AGTACT 1 cut(s) 1099
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.