MD16G1029800.v1.1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004807
Physical Location & Seq
Reverse (-)
2136199 .. 2138321
2123 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1029800.v1.1.491

Sequence Viewer

Length: 1170 bp
ATGACCAAAGACGATGACTTTAAGCTCCTCAAAATCCAGACTTGTATTCTCAAAGTGAACATTCACTGTGATGGTTGTAAGCAGAAAGTCAAGAAACTCCTTCAGAGAATTGAAGGTGTTTACCAAGTGAAAATAGATTCTGATCAGCAGAAAGTCACAGTTTCTGGAAGTGTGGATTCTGCAACTTTGATCAAGAAACTGCTCAGAGCTGGGAAACATGCTGAGATCTGGTCCCAGAAATCTAATAACCAAAGCCAGAAGGGAAACAACAACTGCATCAAAGATGACAAGAACAACAACAACAACAAAGGGCAGAAACAGGGCATCCTCAAGAACTTCGAAGCCTTCAAAAACCAGCAAAAGTTTCCGTCTTTCAGCTCCGAGGAAGATGATGACTTCTTAGACGATGAGGAAGAAAGCGAGGAGGACAAGCTGCGATTTATCCGTGGGATGCTCAGGCAGCAGCAGCAGCAGCAAGCAAATGATGCAAACAATGCAAATGCAAAGAGAAATGCTGGAGTGATCAACGCAGCTGCTGCAGCTCCCAACAATGTGAAGATGATGAACAACATTGTTGAAAGTGTGAATCCCGGGAAGAAAGGTGGGCCCCCGAATCAGAACATGAAGGCGAATCCAGGCGGGATTGATCCGAAAACAATGGCCGCCCTGAAGCTGAACATCAATAACGGCGAAGCTAAAAGAGGAAACATGAATGACCTGAGTGCGATGATGAATTTGGCCGGTTTTCATGGAAATGCCGCTGCAAATGCTGCTGCTTTGGAGGCAAATCCCAATGTTCTTGGAGGGTTTCAGGCTCAGGCAAACCCTAATGCAGCAGGTTTCCCAGGTGGTGGATATACCACGGGGCAAATTGGTCAACAACACCCATCTTCTATCCTGATGAATCCAAATGGGTACACCCACCCATCACAGATGATGAATATGCAGGCTAGGCAAGCAGCAATGCAGCAACAGCCACAGATGATGTATCATAGGTCTCCCTTGGTTCCTCCTAGTACTGGCTACCACAATTACTATCCTAGCCCCGGCCCTACCTTTTCCCCTACAGCCGCCGCATACCCCTACGGCGGTGAGCCTAGTTACGGCAGTAATAACACTGCTGCTCACATGTTCAGTGATGAGAACACCAGCAGCTGTTCAATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

390

Amino Acids

42.31

Weight (kDa)

8.88

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 18 - 73 1.8e-15 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 827
AccB7I CCANNNNNTGG 1 cut(s) 851
AciI CCGC 6 cut(s) 639, 663, 759, 1071, 1074, 1089
AclWI GGATC 1 cut(s) 641
AcoI YGGCCR 2 cut(s) 660, 738
AcsI RAATTY 1 cut(s) 733
AcuI CTGAAG 2 cut(s) 86, 689
AfaI GTAC 2 cut(s) 917, 1018
AfiI CCNNNNNNNGG 5 cut(s) 851, 1019, 1046, 1088, 1103
AflIII ACRYGT 1 cut(s) 1128
AgsI TTSAA 4 cut(s) 113, 349, 578, 1161
AjnI CCWGG 2 cut(s) 634, 844
AluBI AGCT 9 cut(s) 25, 209, 378, 433, 533, 542, 673, 695, 1155
AluI AGCT 9 cut(s) 25, 209, 378, 433, 533, 542, 673, 695, 1155
Alw26I GTCTC 1 cut(s) 1002
AlwI GGATC 1 cut(s) 641
AlwNI CAGNNNCTG 3 cut(s) 164, 536, 1155
Ama87I CYCGRG 1 cut(s) 590
AoxI GGCC 4 cut(s) 605, 660, 738, 1048
ApaI GGGCCC 1 cut(s) 609
ApoI RAATTY 1 cut(s) 733
AspS9I GGNCC 4 cut(s) 231, 605, 606, 1049
AsuC2I CCSGG 3 cut(s) 591, 592, 1047
AsuHPI GGTGA 1 cut(s) 1103
AsuII TTCGAA 1 cut(s) 339
AvaI CYCGRG 1 cut(s) 590
AvaII GGWCC 1 cut(s) 231
BaeGI GKGCMC 1 cut(s) 609
BanII GRGCYC 1 cut(s) 609
BccI CCATC 3 cut(s) 65, 895, 934
BceAI ACGGC 3 cut(s) 703, 1102, 1120
BciT130I CCWGG 2 cut(s) 636, 846
BclI TGATCA 3 cut(s) 142, 189, 522
BcnI CCSGG 3 cut(s) 591, 592, 1047
BcoDI GTCTC 1 cut(s) 1002
BfaI CTAG 4 cut(s) 951, 1014, 1041, 1098
BfmI CTRYAG 2 cut(s) 537, 1065
BfuAI ACCTGC 1 cut(s) 827
BglII AGATCT 1 cut(s) 225
BmcAI AGTACT 1 cut(s) 1018
Bme1390I CCNGG 5 cut(s) 591, 592, 636, 846, 1047
Bme18I GGWCC 1 cut(s) 231
BmeT110I CYCGRG 1 cut(s) 590
BmgT120I GGNCC 4 cut(s) 231, 605, 606, 1049
BmiI GGNNCC 4 cut(s) 233, 607, 608, 1008
BmrFI CCNGG 5 cut(s) 591, 592, 636, 846, 1047
BmsI GCATC 4 cut(s) 285, 333, 441, 475
BpmI CTGGAG 1 cut(s) 537
Bpu10I CCTNAGC 2 cut(s) 455, 816
Bpu14I TTCGAA 1 cut(s) 339
BpuEI CTTGAG 1 cut(s) 314
BpuMI CCSGG 3 cut(s) 591, 592, 1047
BsaBI GATNNNNATC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 1002
BsaJI CCNNGG 7 cut(s) 381, 445, 590, 844, 861, 1002, 1045
Bsc4I CCNNNNNNNGG 5 cut(s) 851, 1019, 1046, 1088, 1103
Bse118I RCCGGY 1 cut(s) 740
Bse1I ACTGG 1 cut(s) 1024
Bse3DI GCAATG 1 cut(s) 969
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 2 cut(s) 636, 846
BseDI CCNNGG 7 cut(s) 381, 445, 590, 844, 861, 1002, 1045
BseGI GGATG 2 cut(s) 324, 456
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 5 cut(s) 851, 1019, 1046, 1088, 1103
BseMI GCAATG 1 cut(s) 969
BseMII CTCAG 5 cut(s) 213, 217, 469, 710, 830
BseNI ACTGG 1 cut(s) 1024
BseRI GAGGAG 2 cut(s) 17, 437
BseSI GKGCMC 1 cut(s) 609
BseYI CCCAGC 1 cut(s) 209
BshFI GGCC 4 cut(s) 607, 662, 740, 1050
BsiHKCI CYCGRG 1 cut(s) 590
BsiSI CCGG 3 cut(s) 591, 741, 1047
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 5 cut(s) 851, 1019, 1046, 1088, 1103
BsmAI GTCTC 1 cut(s) 1002
BsmFI GGGAC 1 cut(s) 217
BsnI GGCC 4 cut(s) 607, 662, 740, 1050
Bso31I GGTCTC 1 cut(s) 1002
BsoBI CYCGRG 1 cut(s) 590
Bsp119I TTCGAA 1 cut(s) 339
Bsp120I GGGCCC 1 cut(s) 605
Bsp1286I GDGCHC 1 cut(s) 609
Bsp143I GATC 5 cut(s) 142, 189, 225, 522, 646
BspACI CCGC 6 cut(s) 639, 663, 759, 1071, 1074, 1089
BspANI GGCC 4 cut(s) 607, 662, 740, 1050
BspCNI CTCAG 5 cut(s) 214, 216, 468, 711, 829
BspLI GGNNCC 4 cut(s) 233, 607, 608, 1008
BspMAI CTGCAG 1 cut(s) 541
BspMI ACCTGC 1 cut(s) 827
BspPI GGATC 1 cut(s) 641
BspT104I TTCGAA 1 cut(s) 339
BspTNI GGTCTC 1 cut(s) 1002
BsrDI GCAATG 1 cut(s) 969
BsrFI RCCGGY 1 cut(s) 740
BsrI ACTGG 1 cut(s) 1024
BssAI RCCGGY 1 cut(s) 740
BssECI CCNNGG 7 cut(s) 381, 445, 590, 844, 861, 1002, 1045
BssMI GATC 5 cut(s) 142, 189, 225, 522, 646
BssT1I CCWWGG 1 cut(s) 1002
Bst2UI CCWGG 2 cut(s) 636, 846
Bst4CI ACNGT 2 cut(s) 68, 160
BstAPI GCANNNNNTGC 4 cut(s) 485, 494, 536, 770
BstBI TTCGAA 1 cut(s) 339
BstC8I GCNNGC 3 cut(s) 477, 948, 957
BstDEI CTNAG 6 cut(s) 203, 222, 400, 455, 719, 816
BstDSI CCRYGG 2 cut(s) 445, 861
BstF5I GGATG 2 cut(s) 324, 456
BstKTI GATC 5 cut(s) 145, 192, 228, 525, 649
BstMAI GTCTC 1 cut(s) 1002
BstMBI GATC 5 cut(s) 142, 189, 225, 522, 646
BstNI CCWGG 2 cut(s) 636, 846
BstNSI RCATGY 2 cut(s) 221, 1132
BstSCI CCNGG 5 cut(s) 589, 590, 634, 844, 1045
BstSFI CTRYAG 2 cut(s) 537, 1065
BstSLI GKGCMC 1 cut(s) 609
BstX2I RGATCY 1 cut(s) 225
BstYI RGATCY 1 cut(s) 225
BsuRI GGCC 4 cut(s) 607, 662, 740, 1050
BtgI CCRYGG 2 cut(s) 445, 861
BtgZI GCGATG 1 cut(s) 740
BtsCI GGATG 2 cut(s) 324, 456
BtsI GCAGTG 1 cut(s) 1116
BtsIMutI CAGTG 3 cut(s) 64, 1116, 1141
BveI ACCTGC 1 cut(s) 827
Cac8I GCNNGC 3 cut(s) 477, 948, 957
CaiI CAGNNNCTG 3 cut(s) 164, 536, 1155
Cfr10I RCCGGY 1 cut(s) 740
Cfr13I GGNCC 4 cut(s) 231, 605, 606, 1049
Cfr9I CCCGGG 1 cut(s) 590
Csp6I GTAC 2 cut(s) 916, 1017
CviAII CATG 6 cut(s) 218, 622, 709, 749, 1129, 1165
CviQI GTAC 2 cut(s) 916, 1017
DdeI CTNAG 6 cut(s) 203, 222, 400, 455, 719, 816
DpnI GATC 5 cut(s) 144, 191, 227, 524, 648
DpnII GATC 5 cut(s) 142, 189, 225, 522, 646
EaeI YGGCCR 2 cut(s) 660, 738
Eco130I CCWWGG 1 cut(s) 1002
Eco24I GRGCYC 1 cut(s) 609
Eco31I GGTCTC 1 cut(s) 1002
Eco47I GGWCC 1 cut(s) 231
Eco57I CTGAAG 2 cut(s) 86, 689
Eco88I CYCGRG 1 cut(s) 590
EcoO109I RGGNCCY 1 cut(s) 606
EcoRII CCWGG 2 cut(s) 634, 844
EcoT14I CCWWGG 1 cut(s) 1002
EcoT38I GRGCYC 1 cut(s) 609
ErhI CCWWGG 1 cut(s) 1002
FaeI CATG 6 cut(s) 221, 625, 712, 752, 1132, 1168
FaqI GGGAC 1 cut(s) 217
FatI CATG 6 cut(s) 217, 621, 708, 748, 1128, 1164
FauI CCCGC 1 cut(s) 632
FbaI TGATCA 3 cut(s) 142, 189, 522
FokI GGATG 2 cut(s) 311, 463
FriOI GRGCYC 1 cut(s) 609
FspBI CTAG 4 cut(s) 951, 1014, 1041, 1098
GsaI CCCAGC 1 cut(s) 213
GsuI CTGGAG 1 cut(s) 537
HaeIII GGCC 4 cut(s) 607, 662, 740, 1050
HapII CCGG 3 cut(s) 591, 741, 1047
Hin1II CATG 6 cut(s) 221, 625, 712, 752, 1132, 1168
HincII GTYRAC 1 cut(s) 878
HindII GTYRAC 1 cut(s) 878
HinfI GANTC 6 cut(s) 137, 176, 586, 613, 631, 904
HpaII CCGG 3 cut(s) 591, 741, 1047
HphI GGTGA 1 cut(s) 1103
Hpy166II GTNNAC 4 cut(s) 58, 121, 878, 918
Hpy188I TCNGA 6 cut(s) 105, 142, 206, 382, 618, 651
Hpy188III TCNNGA 6 cut(s) 37, 91, 165, 193, 331, 898
Hpy8I GTNNAC 4 cut(s) 58, 121, 878, 918
HpyAV CCTTC 5 cut(s) 107, 110, 253, 355, 619
HpyCH4III ACNGT 2 cut(s) 68, 160
HpyF3I CTNAG 6 cut(s) 203, 222, 400, 455, 719, 816
Hsp92II CATG 6 cut(s) 221, 625, 712, 752, 1132, 1168
Ksp22I TGATCA 3 cut(s) 142, 189, 522
Kzo9I GATC 5 cut(s) 142, 189, 225, 522, 646
LmnI GCTCC 3 cut(s) 30, 383, 547
LweI GCATC 4 cut(s) 285, 333, 441, 475
MaeI CTAG 4 cut(s) 951, 1014, 1041, 1098
MaeIII GTNAC 2 cut(s) 154, 1100
MalI GATC 5 cut(s) 144, 191, 227, 524, 648
MboI GATC 5 cut(s) 142, 189, 225, 522, 646
MboII GAAGA 5 cut(s) 398, 425, 568, 607, 882
MflI RGATCY 1 cut(s) 225
MhlI GDGCHC 1 cut(s) 609
MluCI AATT 4 cut(s) 108, 733, 870, 1030
MseI TTAA 1 cut(s) 21
MslI CAYNNNNRTG 2 cut(s) 69, 753
MspA1I CMGCKG 3 cut(s) 533, 761, 1155
MspI CCGG 3 cut(s) 591, 741, 1047
MspR9I CCNGG 5 cut(s) 591, 592, 636, 846, 1047
MvaI CCWGG 2 cut(s) 636, 846
NciI CCSGG 3 cut(s) 591, 592, 1047
NdeII GATC 5 cut(s) 142, 189, 225, 522, 646
NlaIII CATG 6 cut(s) 221, 625, 712, 752, 1132, 1168
NlaIV GGNNCC 4 cut(s) 233, 607, 608, 1008
NmuCI GTSAC 1 cut(s) 154
NspI RCATGY 2 cut(s) 221, 1132
NspV TTCGAA 1 cut(s) 339
PciI ACATGT 1 cut(s) 1128
PfeI GAWTC 6 cut(s) 137, 176, 586, 613, 631, 904
PflMI CCANNNNNTGG 1 cut(s) 851
PscI ACATGT 1 cut(s) 1128
Psp6I CCWGG 2 cut(s) 634, 844
PspFI CCCAGC 1 cut(s) 209
PspGI CCWGG 2 cut(s) 634, 844
PspN4I GGNNCC 4 cut(s) 233, 607, 608, 1008
PspOMI GGGCCC 1 cut(s) 605
PspPI GGNCC 4 cut(s) 231, 605, 606, 1049
PstI CTGCAG 1 cut(s) 541
PstNI CAGNNNCTG 3 cut(s) 164, 536, 1155
PsuI RGATCY 1 cut(s) 225
PvuII CAGCTG 2 cut(s) 533, 1155
RsaI GTAC 2 cut(s) 917, 1018
RsaNI GTAC 2 cut(s) 916, 1017
RseI CAYNNNNRTG 2 cut(s) 69, 753
SaqAI TTAA 1 cut(s) 21
Sau3AI GATC 5 cut(s) 142, 189, 225, 522, 646
Sau96I GGNCC 4 cut(s) 231, 605, 606, 1049
ScaI AGTACT 1 cut(s) 1018
ScrFI CCNGG 5 cut(s) 591, 592, 636, 846, 1047
SduI GDGCHC 1 cut(s) 609
SfaNI GCATC 4 cut(s) 285, 333, 441, 475
SfcI CTRYAG 2 cut(s) 537, 1065
SfuI TTCGAA 1 cut(s) 339
SinI GGWCC 1 cut(s) 231
SmaI CCCGGG 1 cut(s) 592
SmiMI CAYNNNNRTG 2 cut(s) 69, 753
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse9I AATT 4 cut(s) 108, 733, 870, 1030
SsiI CCGC 6 cut(s) 639, 663, 759, 1071, 1074, 1089
SspMI CTAG 4 cut(s) 951, 1014, 1041, 1098
StyD4I CCNGG 5 cut(s) 589, 590, 634, 844, 1045
StyI CCWWGG 1 cut(s) 1002
TaaI ACNGT 2 cut(s) 68, 160
TaqI TCGA 1 cut(s) 339
TasI AATT 4 cut(s) 108, 733, 870, 1030
TatI WGTACW 1 cut(s) 1016
TauI GCSGC 4 cut(s) 665, 761, 1073, 1076
TfiI GAWTC 6 cut(s) 137, 176, 586, 613, 631, 904
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TscAI CASTG 3 cut(s) 71, 1123, 1141
TseFI GTSAC 1 cut(s) 154
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 7 cut(s) 578, 638, 725, 737, 746, 917, 953
TspGWI ACGGA 2 cut(s) 357, 434
TspMI CCCGGG 1 cut(s) 590
TspRI CASTG 3 cut(s) 71, 1123, 1141
Van91I CCANNNNNTGG 1 cut(s) 851
VpaK11BI GGWCC 1 cut(s) 231
XapI RAATTY 1 cut(s) 733
XceI RCATGY 2 cut(s) 221, 1132
XmaI CCCGGG 1 cut(s) 590
XspI CTAG 4 cut(s) 951, 1014, 1041, 1098
ZrmI AGTACT 1 cut(s) 1018
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.