Rw4G000240
ERF Family

heavy metal transport detoxification superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004807
Physical Location & Seq
Reverse (-)
543917 .. 545074
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G000240.1

Sequence Viewer

Length: 894 bp
ATGACTAAAGATGAAGACTTTAAACTCCTCGAGGTCCAGACTTGTGTTCTCAAAGTGAACATACACTTTGATGGGTGTAAGCAGGAAGTAAAGAAACTGCTTCAGAGAATTGAAGGTGTTTACCAAGTCAGCATAGATGCTGATCAGCAGAAAGTCACAGTTTCAGGGGTTATAGACTCTGCCACTTTGATCAAGAAATTGGTTAGAGATGGGACACATGCTGAGCTTTGGTCCCAGCAGAAGGCCAATCAGAACCAAAACCAGAAGCAAAAGAATACCAACTGCATCAAGGACAACAAAGGCCAAAAGCAGCAGGGGTGCATAATAAAGGGCCGTGAAAAAGGTGTTGCGGCTGCAGCTAACCCCAACATTTGTGTCAAGATCACAAACAATCACAATGCTGGGATGAAGGGAGTGCAGCACCCAAAACAGAATGTGCAGCGCATGAAGGTGATTATTAATCCAGGTGGGATTGATCCCAAAACCATGTCACCACTAAACATGAGAAACATTGCTCAATTGGGTGGTGGAAACATCAATTTGGGTGGGGAAGTCAAGAGGGGGATTAACCATGACCTCAGCGCCATGATGAATTTGGCTGGCTTTCATGGCAGTGGCACCGCAAATGCTGCCACTTCTGCTGCTGGTTTAGGGTTCCCTAGTGGTGGTTATGAGATGGGCCAATATCTGCAGACCATGACGATGAACAACACAAATGGCTACGGGCATCCATCGCAGATGATGAACATAAATAAGCAGGCGAGGCAAGCAATGCAGCAGCAGCAAGCTCGGATGATGTATGATCGGTCTCCACTCATTCCTCCTAGTACTACTGGTTATTACCACAACTATGGTTCTACTACTAGTTCTGCTTCATATCTACGGGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

297

Amino Acids

32.52

Weight (kDa)

9.63

Isoelectric Point (pI)

37.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 17 - 68 1.9e-10 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 29
AccB1I GGYRCC 1 cut(s) 617
AciI CCGC 2 cut(s) 350, 621
AclWI GGATC 1 cut(s) 470
AcsI RAATTY 1 cut(s) 592
AcuI CTGAAG 1 cut(s) 86
AfaI GTAC 1 cut(s) 829
AfiI CCNNNNNNNGG 2 cut(s) 241, 665
AgsI TTSAA 1 cut(s) 113
AhlI ACTAGT 1 cut(s) 863
AjnI CCWGG 1 cut(s) 463
AluBI AGCT 3 cut(s) 226, 359, 788
AluI AGCT 3 cut(s) 226, 359, 788
Alw26I GTCTC 1 cut(s) 813
AlwI GGATC 1 cut(s) 470
Ama87I CYCGRG 1 cut(s) 29
AoxI GGCC 4 cut(s) 243, 301, 331, 679
ApoI RAATTY 1 cut(s) 592
AseI ATTAAT 1 cut(s) 459
AspLEI GCGC 2 cut(s) 444, 584
AspS9I GGNCC 4 cut(s) 34, 231, 331, 679
AsuHPI GGTGA 2 cut(s) 463, 483
AvaI CYCGRG 1 cut(s) 29
AvaII GGWCC 2 cut(s) 34, 231
BanI GGYRCC 1 cut(s) 617
BbsI GAAGAC 1 cut(s) 21
BbvCI CCTCAGC 1 cut(s) 578
BccI CCATC 5 cut(s) 65, 203, 670, 739, 882
BceAI ACGGC 1 cut(s) 318
BcgI CGANNNNNNTGC 2 cut(s) 770, 804
BciT130I CCWGG 1 cut(s) 465
BclI TGATCA 2 cut(s) 142, 189
BcoDI GTCTC 1 cut(s) 813
BcuI ACTAGT 1 cut(s) 863
BfaI CTAG 3 cut(s) 660, 825, 864
BfmI CTRYAG 2 cut(s) 354, 689
BfoI RGCGCY 1 cut(s) 585
BlpI GCTNAGC 1 cut(s) 222
BmcAI AGTACT 1 cut(s) 829
Bme1390I CCNGG 1 cut(s) 465
Bme18I GGWCC 2 cut(s) 34, 231
BmeT110I CYCGRG 1 cut(s) 29
BmgT120I GGNCC 4 cut(s) 34, 231, 331, 679
BmiI GGNNCC 3 cut(s) 233, 619, 656
BmrFI CCNGG 1 cut(s) 465
BmsI GCATC 3 cut(s) 127, 294, 736
BpiI GAAGAC 1 cut(s) 21
Bpu10I CCTNAGC 1 cut(s) 578
Bpu1102I GCTNAGC 1 cut(s) 222
BsaBI GATNNNNATC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 813
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 665
Bse1I ACTGG 1 cut(s) 838
Bse3DI GCAATG 2 cut(s) 510, 777
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 1 cut(s) 465
BseGI GGATG 4 cut(s) 411, 727, 798, 893
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 2 cut(s) 241, 665
BseMI GCAATG 2 cut(s) 510, 777
BseMII CTCAG 2 cut(s) 213, 592
BseNI ACTGG 1 cut(s) 838
BseRI GAGGAG 1 cut(s) 17
BseYI CCCAGC 2 cut(s) 234, 401
BsgI GTGCAG 2 cut(s) 437, 458
BshFI GGCC 4 cut(s) 245, 303, 333, 681
BshNI GGYRCC 1 cut(s) 617
BsiHKCI CYCGRG 1 cut(s) 29
BslFI GGGAC 2 cut(s) 217, 226
BslI CCNNNNNNNGG 2 cut(s) 241, 665
BsmAI GTCTC 1 cut(s) 813
BsmFI GGGAC 2 cut(s) 217, 226
BsnI GGCC 4 cut(s) 245, 303, 333, 681
Bso31I GGTCTC 1 cut(s) 813
BsoBI CYCGRG 1 cut(s) 29
Bsp143I GATC 5 cut(s) 142, 189, 381, 475, 802
Bsp1720I GCTNAGC 1 cut(s) 222
BspACI CCGC 2 cut(s) 350, 621
BspANI GGCC 4 cut(s) 245, 303, 333, 681
BspCNI CTCAG 2 cut(s) 214, 591
BspLI GGNNCC 3 cut(s) 233, 619, 656
BspMAI CTGCAG 2 cut(s) 358, 693
BspPI GGATC 1 cut(s) 470
BspT107I GGYRCC 1 cut(s) 617
BspTNI GGTCTC 1 cut(s) 813
BsrDI GCAATG 2 cut(s) 510, 777
BsrI ACTGG 1 cut(s) 838
BssMI GATC 5 cut(s) 142, 189, 381, 475, 802
Bst2UI CCWGG 1 cut(s) 465
Bst4CI ACNGT 1 cut(s) 160
BstAPI GCANNNNNTGC 2 cut(s) 629, 772
BstC8I GCNNGC 4 cut(s) 601, 759, 768, 786
BstDEI CTNAG 2 cut(s) 222, 578
BstF5I GGATG 4 cut(s) 411, 727, 798, 893
BstH2I RGCGCY 1 cut(s) 585
BstHHI GCGC 2 cut(s) 444, 584
BstKTI GATC 5 cut(s) 145, 192, 384, 478, 805
BstMAI GTCTC 1 cut(s) 813
BstMBI GATC 5 cut(s) 142, 189, 381, 475, 802
BstMWI GCNNNNNNNGC 9 cut(s) 356, 609, 629, 638, 733, 763, 767, 772, 781
BstNI CCWGG 1 cut(s) 465
BstNSI RCATGY 1 cut(s) 221
BstSCI CCNGG 1 cut(s) 463
BstSFI CTRYAG 2 cut(s) 354, 689
BstV2I GAAGAC 1 cut(s) 21
BstXI CCANNNNNNTGG 1 cut(s) 851
BsuRI GGCC 4 cut(s) 245, 303, 333, 681
BtgZI GCGATG 1 cut(s) 717
BtsCI GGATG 4 cut(s) 411, 727, 798, 893
BtsI GCAGTG 1 cut(s) 619
BtsIMutI CAGTG 1 cut(s) 619
Cac8I GCNNGC 4 cut(s) 601, 759, 768, 786
CfoI GCGC 2 cut(s) 444, 584
Cfr13I GGNCC 4 cut(s) 34, 231, 331, 679
Csp6I GTAC 1 cut(s) 828
CspCI CAANNNNNGTGG 2 cut(s) 526, 561
CviAII CATG 8 cut(s) 218, 445, 487, 502, 572, 586, 608, 697
CviQI GTAC 1 cut(s) 828
DdeI CTNAG 2 cut(s) 222, 578
DpnI GATC 5 cut(s) 144, 191, 383, 477, 804
DpnII GATC 5 cut(s) 142, 189, 381, 475, 802
DraI TTTAAA 1 cut(s) 22
Eco31I GGTCTC 1 cut(s) 813
Eco47I GGWCC 2 cut(s) 34, 231
Eco57I CTGAAG 1 cut(s) 86
Eco88I CYCGRG 1 cut(s) 29
EcoRII CCWGG 1 cut(s) 463
FaeI CATG 8 cut(s) 221, 448, 490, 505, 575, 589, 611, 700
FaqI GGGAC 2 cut(s) 217, 226
FatI CATG 8 cut(s) 217, 444, 486, 501, 571, 585, 607, 696
FbaI TGATCA 2 cut(s) 142, 189
FokI GGATG 3 cut(s) 418, 714, 805
FspBI CTAG 3 cut(s) 660, 825, 864
GlaI GCGC 2 cut(s) 443, 583
GsaI CCCAGC 2 cut(s) 238, 405
HaeII RGCGCY 1 cut(s) 585
HaeIII GGCC 4 cut(s) 245, 303, 333, 681
HhaI GCGC 2 cut(s) 444, 584
Hin1II CATG 8 cut(s) 221, 448, 490, 505, 575, 589, 611, 700
Hin6I GCGC 2 cut(s) 442, 582
HinP1I GCGC 2 cut(s) 442, 582
HinfI GANTC 1 cut(s) 176
HphI GGTGA 2 cut(s) 463, 483
Hpy166II GTNNAC 2 cut(s) 58, 121
Hpy188I TCNGA 3 cut(s) 105, 252, 792
Hpy188III TCNNGA 4 cut(s) 37, 193, 379, 556
Hpy8I GTNNAC 2 cut(s) 58, 121
HpyAV CCTTC 4 cut(s) 107, 235, 403, 442
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4V TGCA 7 cut(s) 285, 321, 356, 418, 439, 691, 775
HpyF10VI GCNNNNNNNGC 9 cut(s) 356, 609, 629, 638, 733, 763, 767, 772, 781
HpyF3I CTNAG 2 cut(s) 222, 578
Hsp92II CATG 8 cut(s) 221, 448, 490, 505, 575, 589, 611, 700
HspAI GCGC 2 cut(s) 442, 582
Ksp22I TGATCA 2 cut(s) 142, 189
Kzo9I GATC 5 cut(s) 142, 189, 381, 475, 802
LweI GCATC 3 cut(s) 127, 294, 736
MaeI CTAG 3 cut(s) 660, 825, 864
MaeIII GTNAC 2 cut(s) 154, 489
MalI GATC 5 cut(s) 144, 191, 383, 477, 804
MboI GATC 5 cut(s) 142, 189, 381, 475, 802
MboII GAAGA 1 cut(s) 26
MfeI CAATTG 1 cut(s) 518
MluCI AATT 5 cut(s) 108, 197, 518, 538, 592
MlyI GAGTC 1 cut(s) 170
MnlI CCTC 6 cut(s) 25, 38, 552, 587, 756, 831
MseI TTAA 3 cut(s) 21, 459, 567
MslI CAYNNNNRTG 5 cut(s) 69, 449, 612, 701, 849
MspR9I CCNGG 1 cut(s) 465
MunI CAATTG 1 cut(s) 518
MvaI CCWGG 1 cut(s) 465
MwoI GCNNNNNNNGC 9 cut(s) 356, 609, 629, 638, 733, 763, 767, 772, 781
NdeII GATC 5 cut(s) 142, 189, 381, 475, 802
NlaIII CATG 8 cut(s) 221, 448, 490, 505, 575, 589, 611, 700
NlaIV GGNNCC 3 cut(s) 233, 619, 656
NmuCI GTSAC 2 cut(s) 154, 489
NspI RCATGY 1 cut(s) 221
PaeR7I CTCGAG 1 cut(s) 29
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
PshBI ATTAAT 1 cut(s) 459
Psp6I CCWGG 1 cut(s) 463
PspFI CCCAGC 2 cut(s) 234, 401
PspGI CCWGG 1 cut(s) 463
PspN4I GGNNCC 3 cut(s) 233, 619, 656
PspPI GGNCC 4 cut(s) 34, 231, 331, 679
PspXI VCTCGAGB 1 cut(s) 29
PstI CTGCAG 2 cut(s) 358, 693
RsaI GTAC 1 cut(s) 829
RsaNI GTAC 1 cut(s) 828
RseI CAYNNNNRTG 5 cut(s) 69, 449, 612, 701, 849
SaqAI TTAA 3 cut(s) 21, 459, 567
Sau3AI GATC 5 cut(s) 142, 189, 381, 475, 802
Sau96I GGNCC 4 cut(s) 34, 231, 331, 679
ScaI AGTACT 1 cut(s) 829
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 1 cut(s) 465
SetI ASST 9 cut(s) 36, 118, 228, 346, 361, 453, 469, 579, 790
SfaNI GCATC 3 cut(s) 127, 294, 736
SfcI CTRYAG 2 cut(s) 354, 689
Sfr274I CTCGAG 1 cut(s) 29
SinI GGWCC 2 cut(s) 34, 231
SlaI CTCGAG 1 cut(s) 29
SmiMI CAYNNNNRTG 5 cut(s) 69, 449, 612, 701, 849
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
SpeI ACTAGT 1 cut(s) 863
Sse9I AATT 5 cut(s) 108, 197, 518, 538, 592
SsiI CCGC 2 cut(s) 350, 621
SspMI CTAG 3 cut(s) 660, 825, 864
StyD4I CCNGG 1 cut(s) 463
TaaI ACNGT 1 cut(s) 160
TaqI TCGA 1 cut(s) 30
TaqII GACCGA 1 cut(s) 795
TasI AATT 5 cut(s) 108, 197, 518, 538, 592
TatI WGTACW 1 cut(s) 827
TauI GCSGC 1 cut(s) 353
Tru1I TTAA 3 cut(s) 21, 459, 567
Tru9I TTAA 3 cut(s) 21, 459, 567
TscAI CASTG 1 cut(s) 619
TseFI GTSAC 2 cut(s) 154, 489
Tsp45I GTSAC 2 cut(s) 154, 489
TspDTI ATGAA 8 cut(s) 27, 422, 461, 596, 605, 719, 758, 864
TspRI CASTG 1 cut(s) 619
VpaK11BI GGWCC 2 cut(s) 34, 231
VspI ATTAAT 1 cut(s) 459
XapI RAATTY 1 cut(s) 592
XceI RCATGY 1 cut(s) 221
XcmI CCANNNNNNNNNTGG 1 cut(s) 592
XhoI CTCGAG 1 cut(s) 29
XspI CTAG 3 cut(s) 660, 825, 864
ZrmI AGTACT 1 cut(s) 829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.