pycom13g02530

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0004807
Physical Location & Seq
Reverse (-)
1762755 .. 1764437
1683 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g02530.1

Sequence Viewer

Length: 1161 bp
ATGACCAAAGAAGAAGACTTTAAGCTCCTTAAAATCCAGACTTGTGTTCTCAAAGTGAACGTTCACTGTGATGGCTGTAAGCAGAAAGTCAGGAAACTCCTTCAGAGAATTGAAGGTGTTTACCAAGTGAACATAGATTCTGATCAGCAGAAAGTTACAGTTTCTGGAAGTGTGGATTCTGAAACTTTGATCAAGAAATTGCTCAGAGCTGGGAAACATGCAGAGCTCTGGTCCCAGAAATCCAATAACCAAAGCCAGAAGGAAAAGAACAACTGCATCAAAGAAGACAAGAACAACAACAAAGGGCAGAAACAAGGCATCCTCAAAAGCCAGCAAAAGTTTCCGTCTTTCGTCTCCGAGGAAGACGATGACTCCTTCGACGATGAGGAAGAAGACGATGAGGACGAGCTGCGGTTTATCCGGGGGATGCTCAGGCAGCAGCAGCAGCAGCAAGCAAATGATGCAAAGAAAAATGCGGGAGTGATCAATGCAGCAGCTGCTGCAGCTTCCAACAATGTGAAGATGATGAACAATGTTGGAATTGTGAATGCCGGGAAGAAAGGCGGGCATCCGAATGAGAACATGAAGGTGAATACTGGCGGGATTGATCCAAAAACAATGGCTGCCCTGAAGATGAACGCTGCCCAAATGGGCGGCGGAAACATCAATCCCAGCGAAGCGAAAAGAGGCAACATGAATGACCTGAGTGCGATGATGAATTTAGCAGGTTTTCATGGAAATGGTGGAAATGTTGCTGCAAATGCTGCTGCTTTGGGGGCAAATCCCAATGTTCTTGGAGGGTTTCAGGCTCAGGCAAATCCTAACGCAGCAGGGTTCCCCAGTGGTGGATATTCTACCGGGCAAAGTGGTCAATTCCCATCTTCCATGCTGATGAATCCAAATGGGTATGCCCACCCATCACAGATGATGAACATGCAAGCTAGGCAAGCAGCAATGCAACAGCAGCCACAGATGATGTATCATAGGTCGCCCTTGGTTCCTCCTAGTACCGGCTACTACAATTACAATCCTAGCCCTGACCCTAGCTTTTACCCTGCAGCCGCCGCATACCCCTACGGCGGTGAGCCTAGTTACAGCAGCAACAACCCTGCTGCTCATATGTTCAGTGATGAAATCACAAGCAGCTGTCCAATAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

387

Amino Acids

41.79

Weight (kDa)

7.62

Isoelectric Point (pI)

48.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 17 - 73 8.4e-16 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 716
AciI CCGC 9 cut(s) 412, 476, 564, 600, 654, 657, 1062, 1065, 1080
AclI AACGTT 1 cut(s) 60
AclWI GGATC 1 cut(s) 602
AcsI RAATTY 1 cut(s) 718
AcuI CTGAAG 2 cut(s) 86, 650
AfaI GTAC 1 cut(s) 1009
AfiI CCNNNNNNNGG 3 cut(s) 845, 1010, 1079
AgsI TTSAA 1 cut(s) 113
AluBI AGCT 9 cut(s) 25, 209, 226, 409, 497, 506, 941, 1047, 1146
AluI AGCT 9 cut(s) 25, 209, 226, 409, 497, 506, 941, 1047, 1146
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 1 cut(s) 358
AlwI GGATC 1 cut(s) 602
AlwNI CAGNNNCTG 3 cut(s) 164, 497, 500
ApoI RAATTY 1 cut(s) 718
AspS9I GGNCC 1 cut(s) 231
AsuC2I CCSGG 3 cut(s) 422, 553, 859
AsuHPI GGTGA 2 cut(s) 601, 1094
AvaII GGWCC 1 cut(s) 231
BanII GRGCYC 1 cut(s) 228
BbsI GAAGAC 4 cut(s) 21, 291, 369, 399
Bbv12I GWGCWC 1 cut(s) 228
BccI CCATC 3 cut(s) 65, 886, 925
BceAI ACGGC 1 cut(s) 1093
BclI TGATCA 3 cut(s) 142, 189, 483
BcnI CCSGG 3 cut(s) 422, 553, 859
BcoDI GTCTC 1 cut(s) 358
BfaI CTAG 5 cut(s) 942, 1005, 1032, 1044, 1089
BfmI CTRYAG 2 cut(s) 501, 1056
BfuAI ACCTGC 1 cut(s) 716
Bme1390I CCNGG 3 cut(s) 422, 553, 859
Bme18I GGWCC 1 cut(s) 231
BmgT120I GGNCC 1 cut(s) 231
BmiI GGNNCC 3 cut(s) 233, 836, 999
BmrFI CCNGG 3 cut(s) 422, 553, 859
BmrI ACTGGG 1 cut(s) 834
BmsI GCATC 5 cut(s) 285, 327, 417, 451, 577
BmuI ACTGGG 1 cut(s) 834
BpiI GAAGAC 4 cut(s) 21, 291, 369, 399
Bpu10I CCTNAGC 2 cut(s) 431, 810
BpuMI CCSGG 3 cut(s) 422, 553, 859
BsaBI GATNNNNATC 1 cut(s) 141
BsaJI CCNNGG 3 cut(s) 357, 421, 993
BsaXI ACNNNNNCTCC 2 cut(s) 356, 386
Bsc4I CCNNNNNNNGG 3 cut(s) 845, 1010, 1079
Bse118I RCCGGY 1 cut(s) 1010
Bse1I ACTGG 2 cut(s) 601, 840
Bse3DI GCAATG 1 cut(s) 960
Bse8I GATNNNNATC 1 cut(s) 141
BseDI CCNNGG 3 cut(s) 357, 421, 993
BseGI GGATG 3 cut(s) 318, 432, 568
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 3 cut(s) 845, 1010, 1079
BseMI GCAATG 1 cut(s) 960
BseMII CTCAG 4 cut(s) 217, 445, 695, 824
BseNI ACTGG 2 cut(s) 601, 840
BseYI CCCAGC 2 cut(s) 209, 671
BsiHKAI GWGCWC 1 cut(s) 228
BsiSI CCGG 4 cut(s) 421, 552, 858, 1011
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 3 cut(s) 845, 1010, 1079
BsmAI GTCTC 1 cut(s) 358
BsmBI CGTCTC 1 cut(s) 358
BsmFI GGGAC 1 cut(s) 217
BsmI GAATGC 1 cut(s) 553
Bsp1286I GDGCHC 1 cut(s) 228
Bsp143I GATC 4 cut(s) 142, 189, 483, 607
BspACI CCGC 9 cut(s) 412, 476, 564, 600, 654, 657, 1062, 1065, 1080
BspCNI CTCAG 4 cut(s) 216, 444, 696, 823
BspLI GGNNCC 3 cut(s) 233, 836, 999
BspMAI CTGCAG 2 cut(s) 505, 1060
BspMI ACCTGC 1 cut(s) 716
BspPI GGATC 1 cut(s) 602
BsrDI GCAATG 1 cut(s) 960
BsrFI RCCGGY 1 cut(s) 1010
BsrI ACTGG 2 cut(s) 601, 840
BssAI RCCGGY 1 cut(s) 1010
BssECI CCNNGG 3 cut(s) 357, 421, 993
BssMI GATC 4 cut(s) 142, 189, 483, 607
BssT1I CCWWGG 1 cut(s) 993
Bst4CI ACNGT 2 cut(s) 68, 160
BstAPI GCANNNNNTGC 4 cut(s) 461, 497, 500, 764
BstC8I GCNNGC 5 cut(s) 332, 453, 566, 939, 948
BstDEI CTNAG 4 cut(s) 203, 431, 704, 810
BstF5I GGATG 3 cut(s) 318, 432, 568
BstKTI GATC 4 cut(s) 145, 192, 486, 610
BstMAI GTCTC 1 cut(s) 358
BstMBI GATC 4 cut(s) 142, 189, 483, 607
BstNSI RCATGY 2 cut(s) 221, 937
BstSCI CCNGG 3 cut(s) 420, 551, 857
BstSFI CTRYAG 2 cut(s) 501, 1056
BstV2I GAAGAC 4 cut(s) 21, 291, 369, 399
BtgZI GCGATG 1 cut(s) 725
BtsCI GGATG 3 cut(s) 318, 432, 568
BtsIMutI CAGTG 3 cut(s) 64, 847, 1132
BveI ACCTGC 1 cut(s) 716
Cac8I GCNNGC 5 cut(s) 332, 453, 566, 939, 948
CaiI CAGNNNCTG 3 cut(s) 164, 497, 500
Cfr10I RCCGGY 1 cut(s) 1010
Cfr13I GGNCC 1 cut(s) 231
Csp6I GTAC 1 cut(s) 1008
CviAII CATG 6 cut(s) 218, 583, 694, 734, 886, 934
CviQI GTAC 1 cut(s) 1008
DdeI CTNAG 4 cut(s) 203, 431, 704, 810
DpnI GATC 4 cut(s) 144, 191, 485, 609
DpnII GATC 4 cut(s) 142, 189, 483, 607
EciI GGCGGA 1 cut(s) 672
Ecl136II GAGCTC 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 993
Eco24I GRGCYC 1 cut(s) 228
Eco47I GGWCC 1 cut(s) 231
Eco53kI GAGCTC 1 cut(s) 226
Eco57I CTGAAG 2 cut(s) 86, 650
EcoICRI GAGCTC 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 993
EcoT38I GRGCYC 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 993
Esp3I CGTCTC 1 cut(s) 358
FaeI CATG 6 cut(s) 221, 586, 697, 737, 889, 937
FaqI GGGAC 1 cut(s) 217
FatI CATG 6 cut(s) 217, 582, 693, 733, 885, 933
FauI CCCGC 3 cut(s) 469, 557, 593
FauNDI CATATG 1 cut(s) 1119
FbaI TGATCA 3 cut(s) 142, 189, 483
FokI GGATG 3 cut(s) 305, 439, 555
FriOI GRGCYC 1 cut(s) 228
FspBI CTAG 5 cut(s) 942, 1005, 1032, 1044, 1089
GsaI CCCAGC 2 cut(s) 213, 675
HapII CCGG 4 cut(s) 421, 552, 858, 1011
Hin1II CATG 6 cut(s) 221, 586, 697, 737, 889, 937
HinfI GANTC 4 cut(s) 137, 176, 371, 895
HpaII CCGG 4 cut(s) 421, 552, 858, 1011
HphI GGTGA 2 cut(s) 601, 1094
Hpy166II GTNNAC 4 cut(s) 58, 64, 121, 130
Hpy188I TCNGA 6 cut(s) 105, 142, 181, 206, 358, 573
Hpy188III TCNNGA 4 cut(s) 37, 91, 165, 193
Hpy8I GTNNAC 4 cut(s) 58, 64, 121, 130
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 5 cut(s) 107, 110, 253, 385, 580
HpyCH4III ACNGT 2 cut(s) 68, 160
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 9 cut(s) 221, 276, 464, 491, 503, 758, 937, 958, 1058
HpyF3I CTNAG 4 cut(s) 203, 431, 704, 810
HpySE526I ACGT 1 cut(s) 60
Hsp92II CATG 6 cut(s) 221, 586, 697, 737, 889, 937
Ksp22I TGATCA 3 cut(s) 142, 189, 483
Kzo9I GATC 4 cut(s) 142, 189, 483, 607
LmnI GCTCC 1 cut(s) 30
LweI GCATC 5 cut(s) 285, 327, 417, 451, 577
MaeI CTAG 5 cut(s) 942, 1005, 1032, 1044, 1089
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 2 cut(s) 154, 1091
MalI GATC 4 cut(s) 144, 191, 485, 609
MboI GATC 4 cut(s) 142, 189, 483, 607
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 6 cut(s) 108, 197, 540, 718, 872, 1021
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 2 cut(s) 517, 534
MnlI CCTC 7 cut(s) 332, 352, 379, 394, 680, 791, 1011
MseI TTAA 2 cut(s) 21, 30
MslI CAYNNNNRTG 5 cut(s) 69, 573, 587, 738, 890
MspA1I CMGCKG 2 cut(s) 497, 1146
MspI CCGG 4 cut(s) 421, 552, 858, 1011
MspR9I CCNGG 3 cut(s) 422, 553, 859
Mva1269I GAATGC 1 cut(s) 553
NciI CCSGG 3 cut(s) 422, 553, 859
NdeI CATATG 1 cut(s) 1119
NdeII GATC 4 cut(s) 142, 189, 483, 607
NlaIII CATG 6 cut(s) 221, 586, 697, 737, 889, 937
NlaIV GGNNCC 3 cut(s) 233, 836, 999
NspI RCATGY 2 cut(s) 221, 937
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PctI GAATGC 1 cut(s) 553
PfeI GAWTC 3 cut(s) 137, 176, 895
PleI GAGTC 1 cut(s) 365
PpsI GAGTC 1 cut(s) 365
Psp124BI GAGCTC 1 cut(s) 228
Psp1406I AACGTT 1 cut(s) 60
PspFI CCCAGC 2 cut(s) 209, 671
PspN4I GGNNCC 3 cut(s) 233, 836, 999
PspPI GGNCC 1 cut(s) 231
PstI CTGCAG 2 cut(s) 505, 1060
PstNI CAGNNNCTG 3 cut(s) 164, 497, 500
PvuII CAGCTG 2 cut(s) 497, 1146
RsaI GTAC 1 cut(s) 1009
RsaNI GTAC 1 cut(s) 1008
RseI CAYNNNNRTG 5 cut(s) 69, 573, 587, 738, 890
SacI GAGCTC 1 cut(s) 228
SaqAI TTAA 2 cut(s) 21, 30
Sau3AI GATC 4 cut(s) 142, 189, 483, 607
Sau96I GGNCC 1 cut(s) 231
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 3 cut(s) 422, 553, 859
SduI GDGCHC 1 cut(s) 228
SfaNI GCATC 5 cut(s) 285, 327, 417, 451, 577
SfcI CTRYAG 2 cut(s) 501, 1056
SinI GGWCC 1 cut(s) 231
SmiMI CAYNNNNRTG 5 cut(s) 69, 573, 587, 738, 890
Sse9I AATT 6 cut(s) 108, 197, 540, 718, 872, 1021
SsiI CCGC 9 cut(s) 412, 476, 564, 600, 654, 657, 1062, 1065, 1080
SspMI CTAG 5 cut(s) 942, 1005, 1032, 1044, 1089
SstI GAGCTC 1 cut(s) 228
StyD4I CCNGG 3 cut(s) 420, 551, 857
StyI CCWWGG 1 cut(s) 993
TaaI ACNGT 2 cut(s) 68, 160
TaiI ACGT 1 cut(s) 63
TaqI TCGA 1 cut(s) 378
TasI AATT 6 cut(s) 108, 197, 540, 718, 872, 1021
TauI GCSGC 3 cut(s) 657, 1064, 1067
TfiI GAWTC 3 cut(s) 137, 176, 895
Tru1I TTAA 2 cut(s) 21, 30
Tru9I TTAA 2 cut(s) 21, 30
TscAI CASTG 3 cut(s) 71, 847, 1132
TspDTI ATGAA 9 cut(s) 542, 599, 650, 710, 722, 731, 908, 944, 1146
TspGWI ACGGA 1 cut(s) 333
TspRI CASTG 3 cut(s) 71, 847, 1132
VpaK11BI GGWCC 1 cut(s) 231
XapI RAATTY 1 cut(s) 718
XceI RCATGY 2 cut(s) 221, 937
XspI CTAG 5 cut(s) 942, 1005, 1032, 1044, 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.