Rmu_sc0002157.1_g000028
ERF Family

heavy metal transport detoxification superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002157.1
OG0004807
Physical Location & Seq
Forward (+)
121010 .. 121604
595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002157.1_g000028.1.cds

Sequence Viewer

Length: 489 bp
atgaagggagtgcagcacccaaaacagaatgtgcagcgcatgaaggtgattattaatccaggtgggattgatcccaaaaccatgtcaccactaaacatgagaaacattgctcaattgggtggtggaaacatcaatttgggtggggaagtcaagagggggattaaccatgacctcagcgccatgatgaatttggctggctttcatggcagtggcaccgcaaatgctgccacttctgctgctggtttaggtggcaatcctgatgctagtctagcagcattgcaacaagccttcaatctcaacaagctcaagcagcaggcagtggatatcaggctcaagcagattctggaggctagggcgaggcaagcaatgcagcagcagcaagctcggatgatgtatgatcggtctccactcattcctcctagtactactggttattaccacaactatggttctactactagttctgcttcatatctacggggatggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

162

Amino Acids

17.43

Weight (kDa)

10.32

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 212
AciI CCGC 1 cut(s) 216
AclWI GGATC 1 cut(s) 65
AcsI RAATTY 1 cut(s) 187
AfaI GTAC 1 cut(s) 424
AgsI TTSAA 1 cut(s) 292
AhlI ACTAGT 1 cut(s) 458
AjnI CCWGG 1 cut(s) 58
AluBI AGCT 2 cut(s) 304, 383
AluI AGCT 2 cut(s) 304, 383
Alw26I GTCTC 1 cut(s) 408
AlwI GGATC 1 cut(s) 65
AlwNI CAGNNNCTG 1 cut(s) 343
ApeKI GCWGC 9 cut(s) 13, 34, 224, 236, 272, 310, 370, 373, 376
ApoI RAATTY 1 cut(s) 187
AseI ATTAAT 1 cut(s) 54
AspLEI GCGC 2 cut(s) 39, 179
AsuHPI GGTGA 2 cut(s) 58, 78
BanI GGYRCC 1 cut(s) 212
BbvCI CCTCAGC 1 cut(s) 173
BbvI GCAGC 9 cut(s) 25, 46, 211, 223, 284, 322, 382, 385, 388
BccI CCATC 1 cut(s) 477
BcgI CGANNNNNNTGC 2 cut(s) 365, 399
BciT130I CCWGG 1 cut(s) 60
BcoDI GTCTC 1 cut(s) 408
BcuI ACTAGT 1 cut(s) 458
BfaI CTAG 5 cut(s) 264, 269, 351, 420, 459
BfoI RGCGCY 1 cut(s) 180
BisI GCNGC 9 cut(s) 14, 35, 225, 237, 273, 311, 371, 374, 377
BlsI GCNGC 9 cut(s) 15, 36, 226, 238, 274, 312, 372, 375, 378
BmcAI AGTACT 1 cut(s) 424
Bme1390I CCNGG 1 cut(s) 60
BmiI GGNNCC 1 cut(s) 214
BmrFI CCNGG 1 cut(s) 60
BmsI GCATC 1 cut(s) 250
BpmI CTGGAG 1 cut(s) 365
Bpu10I CCTNAGC 1 cut(s) 173
BpuEI CTTGAG 2 cut(s) 290, 317
BsaI GGTCTC 1 cut(s) 408
Bse1I ACTGG 1 cut(s) 433
Bse3DI GCAATG 3 cut(s) 105, 275, 372
BseBI CCWGG 1 cut(s) 60
BseGI GGATG 2 cut(s) 393, 488
BseMI GCAATG 3 cut(s) 105, 275, 372
BseMII CTCAG 1 cut(s) 187
BseNI ACTGG 1 cut(s) 433
BseXI GCAGC 9 cut(s) 25, 46, 211, 223, 284, 322, 382, 385, 388
BsgI GTGCAG 2 cut(s) 32, 53
BshNI GGYRCC 1 cut(s) 212
BsmAI GTCTC 1 cut(s) 408
Bso31I GGTCTC 1 cut(s) 408
Bsp143I GATC 2 cut(s) 70, 397
BspACI CCGC 1 cut(s) 216
BspCNI CTCAG 1 cut(s) 186
BspLI GGNNCC 1 cut(s) 214
BspPI GGATC 1 cut(s) 65
BspT107I GGYRCC 1 cut(s) 212
BspTNI GGTCTC 1 cut(s) 408
BsrDI GCAATG 3 cut(s) 105, 275, 372
BsrI ACTGG 1 cut(s) 433
BssMI GATC 2 cut(s) 70, 397
Bst2UI CCWGG 1 cut(s) 60
BstAPI GCANNNNNTGC 2 cut(s) 224, 367
BstC8I GCNNGC 4 cut(s) 196, 315, 363, 381
BstDEI CTNAG 1 cut(s) 173
BstF5I GGATG 2 cut(s) 393, 488
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 2 cut(s) 39, 179
BstKTI GATC 2 cut(s) 73, 400
BstMAI GTCTC 1 cut(s) 408
BstMBI GATC 2 cut(s) 70, 397
BstMWI GCNNNNNNNGC 8 cut(s) 204, 224, 233, 269, 310, 362, 367, 376
BstNI CCWGG 1 cut(s) 60
BstSCI CCNGG 1 cut(s) 58
BstV1I GCAGC 9 cut(s) 25, 46, 211, 223, 284, 322, 382, 385, 388
BstXI CCANNNNNNTGG 1 cut(s) 446
BtsCI GGATG 2 cut(s) 393, 488
BtsI GCAGTG 2 cut(s) 214, 324
BtsIMutI CAGTG 2 cut(s) 214, 324
Cac8I GCNNGC 4 cut(s) 196, 315, 363, 381
CaiI CAGNNNCTG 1 cut(s) 343
CfoI GCGC 2 cut(s) 39, 179
Csp6I GTAC 1 cut(s) 423
CspCI CAANNNNNGTGG 2 cut(s) 121, 156
CviAII CATG 6 cut(s) 40, 82, 97, 167, 181, 203
CviJI RGCY 7 cut(s) 194, 198, 287, 304, 331, 350, 383
CviKI_1 RGCY 7 cut(s) 194, 198, 287, 304, 331, 350, 383
CviQI GTAC 1 cut(s) 423
DdeI CTNAG 1 cut(s) 173
DpnI GATC 2 cut(s) 72, 399
DpnII GATC 2 cut(s) 70, 397
Eco31I GGTCTC 1 cut(s) 408
Eco32I GATATC 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 58
EcoRV GATATC 1 cut(s) 325
FaeI CATG 6 cut(s) 43, 85, 100, 170, 184, 206
FaiI YATR 9 cut(s) 41, 83, 98, 168, 182, 204, 396, 447, 472
FatI CATG 6 cut(s) 39, 81, 96, 166, 180, 202
Fnu4HI GCNGC 9 cut(s) 14, 35, 225, 237, 273, 311, 371, 374, 377
FokI GGATG 1 cut(s) 400
Fsp4HI GCNGC 9 cut(s) 14, 35, 225, 237, 273, 311, 371, 374, 377
FspBI CTAG 5 cut(s) 264, 269, 351, 420, 459
GlaI GCGC 2 cut(s) 38, 178
GluI GCNGC 9 cut(s) 14, 35, 225, 237, 273, 311, 371, 374, 377
GsuI CTGGAG 1 cut(s) 365
HaeII RGCGCY 1 cut(s) 180
HhaI GCGC 2 cut(s) 39, 179
Hin1II CATG 6 cut(s) 43, 85, 100, 170, 184, 206
Hin6I GCGC 2 cut(s) 37, 177
HinP1I GCGC 2 cut(s) 37, 177
HinfI GANTC 1 cut(s) 340
HphI GGTGA 2 cut(s) 58, 78
Hpy188I TCNGA 1 cut(s) 387
Hpy188III TCNNGA 3 cut(s) 151, 257, 344
HpyAV CCTTC 2 cut(s) 37, 298
HpyCH4V TGCA 4 cut(s) 13, 34, 280, 370
HpyF10VI GCNNNNNNNGC 8 cut(s) 204, 224, 233, 269, 310, 362, 367, 376
HpyF3I CTNAG 1 cut(s) 173
Hsp92II CATG 6 cut(s) 43, 85, 100, 170, 184, 206
HspAI GCGC 2 cut(s) 37, 177
Kzo9I GATC 2 cut(s) 70, 397
LpnPI CCDG 9 cut(s) 45, 72, 180, 225, 270, 299, 313, 329, 414
Lsp1109I GCAGC 9 cut(s) 25, 46, 211, 223, 284, 322, 382, 385, 388
LweI GCATC 1 cut(s) 250
MaeI CTAG 5 cut(s) 264, 269, 351, 420, 459
MaeIII GTNAC 1 cut(s) 84
MalI GATC 2 cut(s) 72, 399
MboI GATC 2 cut(s) 70, 397
MfeI CAATTG 1 cut(s) 113
MluCI AATT 3 cut(s) 113, 133, 187
MnlI CCTC 5 cut(s) 147, 182, 340, 351, 426
MseI TTAA 2 cut(s) 54, 162
MslI CAYNNNNRTG 3 cut(s) 44, 207, 444
MspR9I CCNGG 1 cut(s) 60
MunI CAATTG 1 cut(s) 113
MvaI CCWGG 1 cut(s) 60
MwoI GCNNNNNNNGC 8 cut(s) 204, 224, 233, 269, 310, 362, 367, 376
NdeII GATC 2 cut(s) 70, 397
NlaIII CATG 6 cut(s) 43, 85, 100, 170, 184, 206
NlaIV GGNNCC 1 cut(s) 214
NmuCI GTSAC 1 cut(s) 84
PfeI GAWTC 1 cut(s) 340
PkrI GCNGC 9 cut(s) 15, 36, 226, 238, 274, 312, 372, 375, 378
PshBI ATTAAT 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 58
PspGI CCWGG 1 cut(s) 58
PspN4I GGNNCC 1 cut(s) 214
PstNI CAGNNNCTG 1 cut(s) 343
RsaI GTAC 1 cut(s) 424
RsaNI GTAC 1 cut(s) 423
RseI CAYNNNNRTG 3 cut(s) 44, 207, 444
SaqAI TTAA 2 cut(s) 54, 162
SatI GCNGC 9 cut(s) 14, 35, 225, 237, 273, 311, 371, 374, 377
Sau3AI GATC 2 cut(s) 70, 397
ScaI AGTACT 1 cut(s) 424
ScrFI CCNGG 1 cut(s) 60
SetI ASST 6 cut(s) 48, 64, 174, 250, 306, 385
SfaNI GCATC 1 cut(s) 250
SmiMI CAYNNNNRTG 3 cut(s) 44, 207, 444
SmlI CTYRAG 2 cut(s) 305, 332
SmoI CTYRAG 2 cut(s) 305, 332
SpeI ACTAGT 1 cut(s) 458
Sse9I AATT 3 cut(s) 113, 133, 187
SsiI CCGC 1 cut(s) 216
SspMI CTAG 5 cut(s) 264, 269, 351, 420, 459
StyD4I CCNGG 1 cut(s) 58
TaqII GACCGA 1 cut(s) 390
TasI AATT 3 cut(s) 113, 133, 187
TatI WGTACW 1 cut(s) 422
TfiI GAWTC 1 cut(s) 340
Tru1I TTAA 2 cut(s) 54, 162
Tru9I TTAA 2 cut(s) 54, 162
TscAI CASTG 2 cut(s) 214, 324
TseFI GTSAC 1 cut(s) 84
TseI GCWGC 9 cut(s) 13, 34, 224, 236, 272, 310, 370, 373, 376
Tsp45I GTSAC 1 cut(s) 84
TspDTI ATGAA 5 cut(s) 17, 56, 191, 200, 459
TspRI CASTG 2 cut(s) 214, 324
VspI ATTAAT 1 cut(s) 54
XapI RAATTY 1 cut(s) 187
XcmI CCANNNNNNNNNTGG 1 cut(s) 187
XspI CTAG 5 cut(s) 264, 269, 351, 420, 459
ZrmI AGTACT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.