Rroxscaffold_5G00381970
ERF Family

heavy metal transport detoxification superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004807
Physical Location & Seq
Forward (+)
61827804 .. 61830565
2762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00381970.1

Sequence Viewer

Length: 1239 bp
ATGACTAAAGATGAAGACTTTAAGCTCCTCAAGATCCAGACTTGTGTTCTCAAAGTGAACATACACTGTGATGGGTGTAAGCAGAAAGTGAAGAAACTGCTTCAGAGAATTGAAGGTGTTTACCAAGTCAGCATTGATGCTGATCAGCAGAAAGTAACAATTTCAGGGGTTATAGACTCTGCCACTTTGATCAAGAAATTGGTTAGAGCTGGGAAACATGCTGAGCTTTGGTCCCAGCAGAAGGCCAATCAGAACCAAAACCAGAAGCAAAAGAACACCAACTGCATCAAGGACAACAAAGGCCAAAAGCAGCAGGGGGCGATAATAAAGGGCCTTGAAGCCTTCAAAAACCAGCAGCAGCAGAAGTTCCCCAATTTCCTTATTGAAGAGGATATTGATGACTTCTTGGACGACGATGAGGAGGAGGATGAAGATGATGAGTTGAGGTTTCTGAGGCAGAAAGTGAACCAGCTCAGGCATCAAGCGATTGATGCAAACAACAATGCAAAGAAAGGTGTTGCGGCTGCAGCTAATCCCAACAATAGTGTCAAGATCACTAACAATCACAATGCTGGAAAGAAGGGAGTGCAGCACCCAAATCAGAATGTGCAGGGCATGAAGGTGATTAATCCGGGTGGGATTGATCCCAAAACCATGGCAGCACTGAACCTGGGCAACATTGCTCAAATGGGTGGTGGAAACATCAATTTGGGTGGGGAAGTCAAGAGGGGGAATAACCATGACCTCAGTGCCATGATGAATTTGGCTGGCTTTCATGGCAATGGTGCCACAAATGCTGCTACGTCTGCTGCTGGTATGGGTGGCAATCCCAATGTTAGTCTAGCAGCATTGCAACAAGCGATTCAATCTCAGCAAGCTCATGCTGTAGGCAGTGGATATCAGGCACAAGCAGCTTCTGGAGGGTTCCCTAGTGGTGGTTATGTGACAGGCCAATATCCGCAGACTATGATGATGAACACAAATGGCTACGGGCATCCATCGCAGATGATGAACATGAATATGCAGGCGAGGCAAGCAATGCAGCAGCAACCTCAGATGATGTATCATAGGTCTCCATTCAGTCCTCCTAGTACTACTGGTTATTTCCACAACTATGGTCCTGCTCCTAGTTCTGTTCCATATCCATACAGTGCTGAGCCTGATTACAGCAGCGGTGATGGCAACAATGCAGCTCATATGTTCAATGATGAGAACACCAGCAGCTGTTCTATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

412

Amino Acids

44.75

Weight (kDa)

8.14

Isoelectric Point (pI)

36.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 17 - 73 6.5e-15 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 785
AciI CCGC 3 cut(s) 521, 959, 1173
AclWI GGATC 2 cut(s) 28, 638
AcsI RAATTY 1 cut(s) 760
AcuI CTGAAG 1 cut(s) 86
AfaI GTAC 1 cut(s) 1093
AfiI CCNNNNNNNGG 2 cut(s) 241, 935
AgsI TTSAA 6 cut(s) 113, 338, 346, 386, 866, 1204
AjnI CCWGG 1 cut(s) 669
AluBI AGCT 9 cut(s) 25, 209, 226, 472, 530, 878, 914, 1193, 1224
AluI AGCT 9 cut(s) 25, 209, 226, 472, 530, 878, 914, 1193, 1224
Alw26I GTCTC 1 cut(s) 1077
AlwI GGATC 2 cut(s) 28, 638
AlwNI CAGNNNCTG 2 cut(s) 917, 1224
AoxI GGCC 4 cut(s) 243, 301, 331, 949
ApoI RAATTY 1 cut(s) 760
AseI ATTAAT 1 cut(s) 627
AspS9I GGNCC 3 cut(s) 231, 331, 1118
AsuC2I CCSGG 1 cut(s) 633
AsuHPI GGTGA 2 cut(s) 634, 1187
AvaII GGWCC 2 cut(s) 231, 1118
BanI GGYRCC 1 cut(s) 785
BbsI GAAGAC 1 cut(s) 21
BccI CCATC 3 cut(s) 65, 1006, 1172
BciT130I CCWGG 1 cut(s) 671
BclI TGATCA 2 cut(s) 142, 189
BcnI CCSGG 1 cut(s) 633
BcoDI GTCTC 1 cut(s) 1077
BfaI CTAG 4 cut(s) 842, 930, 1089, 1128
BfmI CTRYAG 2 cut(s) 525, 885
BlpI GCTNAGC 2 cut(s) 222, 1155
BmcAI AGTACT 1 cut(s) 1093
Bme1390I CCNGG 2 cut(s) 633, 671
Bme18I GGWCC 2 cut(s) 231, 1118
BmgT120I GGNCC 3 cut(s) 231, 331, 1118
BmiI GGNNCC 3 cut(s) 233, 787, 926
BmrFI CCNGG 2 cut(s) 633, 671
BmsI GCATC 5 cut(s) 127, 294, 481, 487, 1003
BpiI GAAGAC 1 cut(s) 21
BpmI CTGGAG 1 cut(s) 939
Bpu10I CCTNAGC 1 cut(s) 473
Bpu1102I GCTNAGC 2 cut(s) 222, 1155
BpuEI CTTGAG 1 cut(s) 14
BpuMI CCSGG 1 cut(s) 633
BsaBI GATNNNNATC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 1077
BsaJI CCNNGG 2 cut(s) 654, 670
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 935
Bse1I ACTGG 1 cut(s) 1102
Bse3DI GCAATG 4 cut(s) 678, 787, 848, 1044
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 1 cut(s) 671
BseDI CCNNGG 2 cut(s) 654, 670
BseGI GGATG 2 cut(s) 433, 994
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 2 cut(s) 241, 935
BseMI GCAATG 4 cut(s) 678, 787, 848, 1044
BseMII CTCAG 7 cut(s) 213, 443, 487, 760, 884, 1067, 1146
BseNI ACTGG 1 cut(s) 1102
BseRI GAGGAG 3 cut(s) 17, 434, 437
BseYI CCCAGC 2 cut(s) 209, 234
BsgI GTGCAG 2 cut(s) 608, 629
BshFI GGCC 4 cut(s) 245, 303, 333, 951
BshNI GGYRCC 1 cut(s) 785
BsiSI CCGG 1 cut(s) 632
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 2 cut(s) 241, 935
BsmAI GTCTC 1 cut(s) 1077
BsmFI GGGAC 1 cut(s) 217
BsnI GGCC 4 cut(s) 245, 303, 333, 951
Bso31I GGTCTC 1 cut(s) 1077
Bsp143I GATC 5 cut(s) 33, 142, 189, 552, 643
Bsp1720I GCTNAGC 2 cut(s) 222, 1155
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 3 cut(s) 521, 959, 1173
BspANI GGCC 4 cut(s) 245, 303, 333, 951
BspCNI CTCAG 7 cut(s) 214, 444, 486, 759, 883, 1066, 1147
BspLI GGNNCC 3 cut(s) 233, 787, 926
BspMAI CTGCAG 1 cut(s) 529
BspPI GGATC 2 cut(s) 28, 638
BspT107I GGYRCC 1 cut(s) 785
BspTNI GGTCTC 1 cut(s) 1077
BsrDI GCAATG 4 cut(s) 678, 787, 848, 1044
BsrI ACTGG 1 cut(s) 1102
BssECI CCNNGG 2 cut(s) 654, 670
BssMI GATC 5 cut(s) 33, 142, 189, 552, 643
BssT1I CCWWGG 1 cut(s) 654
Bst2UI CCWGG 1 cut(s) 671
Bst4CI ACNGT 2 cut(s) 68, 1151
Bst6I CTCTTC 1 cut(s) 381
BstAPI GCANNNNNTGC 1 cut(s) 1039
BstC8I GCNNGC 4 cut(s) 769, 876, 1026, 1035
BstDEI CTNAG 7 cut(s) 222, 452, 473, 746, 870, 1053, 1155
BstDSI CCRYGG 1 cut(s) 654
BstF5I GGATG 2 cut(s) 433, 994
BstKTI GATC 5 cut(s) 36, 145, 192, 555, 646
BstMAI GTCTC 1 cut(s) 1077
BstMBI GATC 5 cut(s) 33, 142, 189, 552, 643
BstNI CCWGG 1 cut(s) 671
BstNSI RCATGY 1 cut(s) 221
BstSCI CCNGG 2 cut(s) 631, 669
BstSFI CTRYAG 2 cut(s) 525, 885
BstV2I GAAGAC 1 cut(s) 21
BstX2I RGATCY 1 cut(s) 33
BstXI CCANNNNNNTGG 2 cut(s) 655, 1115
BstYI RGATCY 1 cut(s) 33
BsuRI GGCC 4 cut(s) 245, 303, 333, 951
BtgI CCRYGG 1 cut(s) 654
BtgZI GCGATG 1 cut(s) 984
BtsCI GGATG 2 cut(s) 433, 994
BtsI GCAGTG 1 cut(s) 898
BtsIMutI CAGTG 5 cut(s) 64, 662, 754, 898, 1156
Cac8I GCNNGC 4 cut(s) 769, 876, 1026, 1035
CaiI CAGNNNCTG 2 cut(s) 917, 1224
Cfr13I GGNCC 3 cut(s) 231, 331, 1118
Csp6I GTAC 1 cut(s) 1092
CspCI CAANNNNNGTGG 2 cut(s) 694, 729
CviAII CATG 9 cut(s) 218, 616, 655, 740, 754, 776, 881, 1015, 1234
CviQI GTAC 1 cut(s) 1092
DdeI CTNAG 7 cut(s) 222, 452, 473, 746, 870, 1053, 1155
DpnI GATC 5 cut(s) 35, 144, 191, 554, 645
DpnII GATC 5 cut(s) 33, 142, 189, 552, 643
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
Eco130I CCWWGG 1 cut(s) 654
Eco31I GGTCTC 1 cut(s) 1077
Eco32I GATATC 1 cut(s) 899
Eco47I GGWCC 2 cut(s) 231, 1118
Eco57I CTGAAG 1 cut(s) 86
EcoO109I RGGNCCY 1 cut(s) 331
EcoRII CCWGG 1 cut(s) 669
EcoRV GATATC 1 cut(s) 899
EcoT14I CCWWGG 1 cut(s) 654
ErhI CCWWGG 1 cut(s) 654
FaeI CATG 9 cut(s) 221, 619, 658, 743, 757, 779, 884, 1018, 1237
FaqI GGGAC 1 cut(s) 217
FatI CATG 9 cut(s) 217, 615, 654, 739, 753, 775, 880, 1014, 1233
FauNDI CATATG 1 cut(s) 1197
FbaI TGATCA 2 cut(s) 142, 189
FokI GGATG 2 cut(s) 440, 981
FspBI CTAG 4 cut(s) 842, 930, 1089, 1128
GsaI CCCAGC 2 cut(s) 213, 238
GsuI CTGGAG 1 cut(s) 939
HaeIII GGCC 4 cut(s) 245, 303, 333, 951
HapII CCGG 1 cut(s) 632
Hin1II CATG 9 cut(s) 221, 619, 658, 743, 757, 779, 884, 1018, 1237
HinfI GANTC 2 cut(s) 176, 862
HpaII CCGG 1 cut(s) 632
HphI GGTGA 2 cut(s) 634, 1187
Hpy166II GTNNAC 3 cut(s) 58, 121, 466
Hpy188I TCNGA 5 cut(s) 105, 252, 453, 603, 1056
Hpy188III TCNNGA 6 cut(s) 31, 37, 193, 550, 724, 918
Hpy8I GTNNAC 3 cut(s) 58, 121, 466
Hpy99I CGWCG 1 cut(s) 416
HpyAV CCTTC 5 cut(s) 107, 235, 352, 574, 613
HpyCH4III ACNGT 2 cut(s) 68, 1151
HpyCH4IV ACGT 1 cut(s) 803
HpyF3I CTNAG 7 cut(s) 222, 452, 473, 746, 870, 1053, 1155
HpySE526I ACGT 1 cut(s) 803
Hsp92II CATG 9 cut(s) 221, 619, 658, 743, 757, 779, 884, 1018, 1237
Ksp22I TGATCA 2 cut(s) 142, 189
Kzo9I GATC 5 cut(s) 33, 142, 189, 552, 643
LmnI GCTCC 2 cut(s) 30, 1129
LweI GCATC 5 cut(s) 127, 294, 481, 487, 1003
MaeI CTAG 4 cut(s) 842, 930, 1089, 1128
MaeII ACGT 1 cut(s) 803
MaeIII GTNAC 2 cut(s) 154, 943
MalI GATC 5 cut(s) 35, 144, 191, 554, 645
MboI GATC 5 cut(s) 33, 142, 189, 552, 643
MboII GAAGA 4 cut(s) 26, 103, 398, 443
MflI RGATCY 1 cut(s) 33
MluCI AATT 6 cut(s) 108, 159, 197, 373, 706, 760
MlyI GAGTC 1 cut(s) 170
MseI TTAA 2 cut(s) 21, 627
MslI CAYNNNNRTG 5 cut(s) 69, 620, 780, 1019, 1113
MspA1I CMGCKG 2 cut(s) 1173, 1224
MspI CCGG 1 cut(s) 632
MspR9I CCNGG 2 cut(s) 633, 671
MvaI CCWGG 1 cut(s) 671
NciI CCSGG 1 cut(s) 633
NcoI CCATGG 1 cut(s) 654
NdeI CATATG 1 cut(s) 1197
NdeII GATC 5 cut(s) 33, 142, 189, 552, 643
NlaIII CATG 9 cut(s) 221, 619, 658, 743, 757, 779, 884, 1018, 1237
NlaIV GGNNCC 3 cut(s) 233, 787, 926
NmuCI GTSAC 1 cut(s) 943
NspI RCATGY 1 cut(s) 221
PfeI GAWTC 1 cut(s) 862
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
PshBI ATTAAT 1 cut(s) 627
Psp6I CCWGG 1 cut(s) 669
PspFI CCCAGC 2 cut(s) 209, 234
PspGI CCWGG 1 cut(s) 669
PspN4I GGNNCC 3 cut(s) 233, 787, 926
PspPI GGNCC 3 cut(s) 231, 331, 1118
PstI CTGCAG 1 cut(s) 529
PstNI CAGNNNCTG 2 cut(s) 917, 1224
PsuI RGATCY 1 cut(s) 33
PvuII CAGCTG 1 cut(s) 1224
RsaI GTAC 1 cut(s) 1093
RsaNI GTAC 1 cut(s) 1092
RseI CAYNNNNRTG 5 cut(s) 69, 620, 780, 1019, 1113
SaqAI TTAA 2 cut(s) 21, 627
Sau3AI GATC 5 cut(s) 33, 142, 189, 552, 643
Sau96I GGNCC 3 cut(s) 231, 331, 1118
ScaI AGTACT 1 cut(s) 1093
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 2 cut(s) 633, 671
SfaNI GCATC 5 cut(s) 127, 294, 481, 487, 1003
SfcI CTRYAG 2 cut(s) 525, 885
SinI GGWCC 2 cut(s) 231, 1118
SmiMI CAYNNNNRTG 5 cut(s) 69, 620, 780, 1019, 1113
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 6 cut(s) 108, 159, 197, 373, 706, 760
SsiI CCGC 3 cut(s) 521, 959, 1173
SspMI CTAG 4 cut(s) 842, 930, 1089, 1128
StyD4I CCNGG 2 cut(s) 631, 669
StyI CCWWGG 1 cut(s) 654
TaaI ACNGT 2 cut(s) 68, 1151
TaiI ACGT 1 cut(s) 806
TasI AATT 6 cut(s) 108, 159, 197, 373, 706, 760
TatI WGTACW 1 cut(s) 1091
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 1 cut(s) 862
Tru1I TTAA 2 cut(s) 21, 627
Tru9I TTAA 2 cut(s) 21, 627
TscAI CASTG 5 cut(s) 71, 669, 754, 898, 1156
TseFI GTSAC 1 cut(s) 943
Tsp45I GTSAC 1 cut(s) 943
TspDTI ATGAA 8 cut(s) 27, 444, 632, 764, 773, 989, 1025, 1031
TspRI CASTG 5 cut(s) 71, 669, 754, 898, 1156
VpaK11BI GGWCC 2 cut(s) 231, 1118
VspI ATTAAT 1 cut(s) 627
XapI RAATTY 1 cut(s) 760
XceI RCATGY 1 cut(s) 221
XcmI CCANNNNNNNNNTGG 1 cut(s) 760
XspI CTAG 4 cut(s) 842, 930, 1089, 1128
ZrmI AGTACT 1 cut(s) 1093
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.