RLG00000006124
ERF Family

heavy metal transport detoxification superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004807
Physical Location & Seq
Reverse (-)
4609746 .. 4611694
1949 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006124

Sequence Viewer

Length: 1227 bp
ATGACTAAAGATGAAGACTTTAAGCTCCTCAAGATCCAGACTTGTGTTCTCAAAGTGAACATACACTGTGATGGGTGTAAGCAGAAAGTGAAGAAACTGCTTCAGAGAATTGAAGGTGTTTACCAAGTCAGCATTGATGCTGATCAGCAGAAAGTAACAATTTCAGGGGTTATAGACTCTGCCACTTTGATCAAGAAATTGGTTAGAGCTGGGAAACATGCTGAGCTTTGGTCCCAGCAGAAGGCCAATCAGAATCAAAACCAGAAGCAAAAGAACACCAACTGCATCAAGGACAGCAAAGTCCAAAAGCAGCAGGGGGGGATAATAAAGGGCCTTGAAGCCTTCAAAAACCAGCAGCAGCAGAAGTTCCCCAATTTCCTTATTGAAGAGGATATTGATGACTTCTTGGACGACGATGAGGAGGAGGATGAAGATGATGAGTTTAGGTTTCTGAGGCAGAAAGTGAACCAGCTCAGGCATCAAGCGATTGATGCAAACAACAATGCGAAGAAAGGTGTTGCGGCTGCAGCTAATCCCAACAATAGTGTCAAGATCACAAACAATCACAATGCTGGAAAGAAGGGAGTGCAGCACCCAAATCAGAATGTGCAGGGCATGAAGGTGATTAATCCGGGCGGGATTGATCCCAAAACCATGGCAGCACTGAACCTGGGCAACATTGCTCAATTGGGTGGTGGAAACATCAATTTGGGTGGGGAAGTCAAGAGGGGGAATAACCATGACCTCAGTGCCATGATGAATTTGGCTGGCTTTCATGGCAATGGTGCAGCAAATGCTGCTACTTCTGCTGCTGGTATGGGTGGCAATCCCAATGTTAGTCTAGCAGCATTGCAACAAGCGCTTCAATCTCAGCAAGCTCAAGCTGCAGGCAGTGGATATCAGGCTCAAGCAGCTTCTGGAGGGTTCCCTAGTGGTGGTTATCCGCAGACCATGATGATGAACACAAATGGCTACGGTCATCCATCGCAGATGATGAACATGAATATGCAGGCGAGGCAAGCAATGCAGCAGCAGCAACCCCAGATGATGTATCATAGGTCTCCATTCAGTCCTCCTAGTACTACTGGTTATTACCACAACTATGGCCCTGCTCCTAGTTCTGTTCCGTATCCATACAGTGCTGAGCCTGATTACAGCAGCGGTGATGGCAACAATGCAGCTCATATGTTCAATGATGAGAACACCAGCAGCTGTTCTATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

409

Amino Acids

44.3

Weight (kDa)

8.13

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 17 - 73 6.4e-15 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 299
AciI CCGC 4 cut(s) 521, 636, 944, 1161
AclWI GGATC 2 cut(s) 28, 638
AcsI RAATTY 1 cut(s) 760
AcuI CTGAAG 1 cut(s) 86
AfaI GTAC 1 cut(s) 1081
AfeI AGCGCT 1 cut(s) 861
AfiI CCNNNNNNNGG 2 cut(s) 241, 935
AgsI TTSAA 6 cut(s) 113, 338, 346, 386, 866, 1192
AjnI CCWGG 1 cut(s) 669
Alw26I GTCTC 1 cut(s) 1065
AlwI GGATC 2 cut(s) 28, 638
AlwNI CAGNNNCTG 2 cut(s) 917, 1212
Aor51HI AGCGCT 1 cut(s) 861
AoxI GGCC 3 cut(s) 243, 331, 1105
ApoI RAATTY 1 cut(s) 760
AseI ATTAAT 1 cut(s) 627
AspLEI GCGC 1 cut(s) 862
AspS9I GGNCC 3 cut(s) 231, 331, 1106
AsuC2I CCSGG 1 cut(s) 633
AsuHPI GGTGA 2 cut(s) 634, 1175
AvaII GGWCC 1 cut(s) 231
BbsI GAAGAC 1 cut(s) 21
BccI CCATC 3 cut(s) 65, 991, 1160
BciT130I CCWGG 1 cut(s) 671
BciVI GTATCC 1 cut(s) 1140
BclI TGATCA 2 cut(s) 142, 189
BcnI CCSGG 1 cut(s) 633
BcoDI GTCTC 1 cut(s) 1065
BfaI CTAG 4 cut(s) 842, 930, 1077, 1116
BfmI CTRYAG 2 cut(s) 525, 885
BfoI RGCGCY 1 cut(s) 863
BfuI GTATCC 1 cut(s) 1140
BlpI GCTNAGC 2 cut(s) 222, 1143
BmcAI AGTACT 1 cut(s) 1081
Bme1390I CCNGG 2 cut(s) 633, 671
Bme18I GGWCC 1 cut(s) 231
BmgT120I GGNCC 3 cut(s) 231, 331, 1106
BmiI GGNNCC 2 cut(s) 233, 926
BmrFI CCNGG 2 cut(s) 633, 671
BmsI GCATC 4 cut(s) 127, 294, 481, 487
BpiI GAAGAC 1 cut(s) 21
BpmI CTGGAG 1 cut(s) 939
Bpu10I CCTNAGC 1 cut(s) 473
Bpu1102I GCTNAGC 2 cut(s) 222, 1143
BpuEI CTTGAG 3 cut(s) 14, 864, 891
BpuMI CCSGG 1 cut(s) 633
BsaBI GATNNNNATC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 1065
BsaJI CCNNGG 2 cut(s) 654, 670
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 935
Bse1I ACTGG 1 cut(s) 1090
Bse3DI GCAATG 4 cut(s) 678, 787, 848, 1029
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 1 cut(s) 671
BseDI CCNNGG 2 cut(s) 654, 670
BseGI GGATG 2 cut(s) 433, 979
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 2 cut(s) 241, 935
BseMI GCAATG 4 cut(s) 678, 787, 848, 1029
BseMII CTCAG 6 cut(s) 213, 443, 487, 760, 884, 1134
BseNI ACTGG 1 cut(s) 1090
BseRI GAGGAG 3 cut(s) 17, 434, 437
BseYI CCCAGC 2 cut(s) 209, 234
BsgI GTGCAG 3 cut(s) 608, 629, 807
BshFI GGCC 3 cut(s) 245, 333, 1107
BsiSI CCGG 1 cut(s) 632
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 2 cut(s) 241, 935
BsmAI GTCTC 1 cut(s) 1065
BsmFI GGGAC 1 cut(s) 217
BsnI GGCC 3 cut(s) 245, 333, 1107
Bso31I GGTCTC 1 cut(s) 1065
Bsp143I GATC 5 cut(s) 33, 142, 189, 552, 643
Bsp1720I GCTNAGC 2 cut(s) 222, 1143
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 4 cut(s) 521, 636, 944, 1161
BspANI GGCC 3 cut(s) 245, 333, 1107
BspCNI CTCAG 6 cut(s) 214, 444, 486, 759, 883, 1135
BspLI GGNNCC 2 cut(s) 233, 926
BspMAI CTGCAG 2 cut(s) 529, 889
BspPI GGATC 2 cut(s) 28, 638
BspTNI GGTCTC 1 cut(s) 1065
BsrDI GCAATG 4 cut(s) 678, 787, 848, 1029
BsrI ACTGG 1 cut(s) 1090
BssECI CCNNGG 2 cut(s) 654, 670
BssMI GATC 5 cut(s) 33, 142, 189, 552, 643
BssT1I CCWWGG 1 cut(s) 654
Bst2UI CCWGG 1 cut(s) 671
Bst4CI ACNGT 3 cut(s) 68, 977, 1139
Bst6I CTCTTC 1 cut(s) 381
BstAPI GCANNNNNTGC 3 cut(s) 794, 797, 1024
BstC8I GCNNGC 5 cut(s) 769, 876, 889, 1011, 1020
BstDEI CTNAG 6 cut(s) 222, 452, 473, 746, 870, 1143
BstDSI CCRYGG 1 cut(s) 654
BstF5I GGATG 2 cut(s) 433, 979
BstH2I RGCGCY 1 cut(s) 863
BstHHI GCGC 1 cut(s) 862
BstKTI GATC 5 cut(s) 36, 145, 192, 555, 646
BstMAI GTCTC 1 cut(s) 1065
BstMBI GATC 5 cut(s) 33, 142, 189, 552, 643
BstNI CCWGG 1 cut(s) 671
BstNSI RCATGY 1 cut(s) 221
BstSCI CCNGG 2 cut(s) 631, 669
BstSFI CTRYAG 2 cut(s) 525, 885
BstV2I GAAGAC 1 cut(s) 21
BstX2I RGATCY 1 cut(s) 33
BstXI CCANNNNNNTGG 2 cut(s) 655, 1103
BstYI RGATCY 1 cut(s) 33
BsuI GTATCC 1 cut(s) 1140
BsuRI GGCC 3 cut(s) 245, 333, 1107
BtgI CCRYGG 1 cut(s) 654
BtgZI GCGATG 1 cut(s) 969
BtsCI GGATG 2 cut(s) 433, 979
BtsI GCAGTG 1 cut(s) 898
BtsIMutI CAGTG 5 cut(s) 64, 662, 754, 898, 1144
Cac8I GCNNGC 5 cut(s) 769, 876, 889, 1011, 1020
CaiI CAGNNNCTG 2 cut(s) 917, 1212
CfoI GCGC 1 cut(s) 862
Cfr13I GGNCC 3 cut(s) 231, 331, 1106
Csp6I GTAC 1 cut(s) 1080
CspCI CAANNNNNGTGG 2 cut(s) 694, 729
CviAII CATG 9 cut(s) 218, 616, 655, 740, 754, 776, 952, 1000, 1222
CviQI GTAC 1 cut(s) 1080
DdeI CTNAG 6 cut(s) 222, 452, 473, 746, 870, 1143
DpnI GATC 5 cut(s) 35, 144, 191, 554, 645
DpnII GATC 5 cut(s) 33, 142, 189, 552, 643
DrdI GACNNNNNNGTC 1 cut(s) 299
DseDI GACNNNNNNGTC 1 cut(s) 299
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
Eco130I CCWWGG 1 cut(s) 654
Eco31I GGTCTC 1 cut(s) 1065
Eco32I GATATC 1 cut(s) 899
Eco47I GGWCC 1 cut(s) 231
Eco47III AGCGCT 1 cut(s) 861
Eco57I CTGAAG 1 cut(s) 86
EcoO109I RGGNCCY 1 cut(s) 331
EcoRII CCWGG 1 cut(s) 669
EcoRV GATATC 1 cut(s) 899
EcoT14I CCWWGG 1 cut(s) 654
ErhI CCWWGG 1 cut(s) 654
FaeI CATG 9 cut(s) 221, 619, 658, 743, 757, 779, 955, 1003, 1225
FaqI GGGAC 1 cut(s) 217
FatI CATG 9 cut(s) 217, 615, 654, 739, 753, 775, 951, 999, 1221
FauI CCCGC 1 cut(s) 629
FauNDI CATATG 1 cut(s) 1185
FbaI TGATCA 2 cut(s) 142, 189
FokI GGATG 2 cut(s) 440, 966
FspBI CTAG 4 cut(s) 842, 930, 1077, 1116
GlaI GCGC 1 cut(s) 861
GsaI CCCAGC 2 cut(s) 213, 238
GsuI CTGGAG 1 cut(s) 939
HaeII RGCGCY 1 cut(s) 863
HaeIII GGCC 3 cut(s) 245, 333, 1107
HapII CCGG 1 cut(s) 632
HhaI GCGC 1 cut(s) 862
Hin1II CATG 9 cut(s) 221, 619, 658, 743, 757, 779, 955, 1003, 1225
Hin6I GCGC 1 cut(s) 860
HinP1I GCGC 1 cut(s) 860
HinfI GANTC 2 cut(s) 176, 253
HpaII CCGG 1 cut(s) 632
HphI GGTGA 2 cut(s) 634, 1175
Hpy166II GTNNAC 3 cut(s) 58, 121, 466
Hpy188I TCNGA 4 cut(s) 105, 252, 453, 603
Hpy188III TCNNGA 6 cut(s) 31, 37, 193, 550, 724, 918
Hpy8I GTNNAC 3 cut(s) 58, 121, 466
Hpy99I CGWCG 1 cut(s) 416
HpyAV CCTTC 5 cut(s) 107, 235, 352, 574, 613
HpyCH4III ACNGT 3 cut(s) 68, 977, 1139
HpyF3I CTNAG 6 cut(s) 222, 452, 473, 746, 870, 1143
Hsp92II CATG 9 cut(s) 221, 619, 658, 743, 757, 779, 955, 1003, 1225
HspAI GCGC 1 cut(s) 860
Ksp22I TGATCA 2 cut(s) 142, 189
Kzo9I GATC 5 cut(s) 33, 142, 189, 552, 643
LmnI GCTCC 2 cut(s) 30, 1117
LweI GCATC 4 cut(s) 127, 294, 481, 487
MaeI CTAG 4 cut(s) 842, 930, 1077, 1116
MaeIII GTNAC 1 cut(s) 154
MalI GATC 5 cut(s) 35, 144, 191, 554, 645
MboI GATC 5 cut(s) 33, 142, 189, 552, 643
MboII GAAGA 5 cut(s) 26, 103, 398, 443, 520
MfeI CAATTG 1 cut(s) 686
MflI RGATCY 1 cut(s) 33
MluCI AATT 7 cut(s) 108, 159, 197, 373, 686, 706, 760
MlyI GAGTC 1 cut(s) 170
MseI TTAA 2 cut(s) 21, 627
MslI CAYNNNNRTG 6 cut(s) 69, 620, 780, 956, 1004, 1101
MspA1I CMGCKG 2 cut(s) 1161, 1212
MspI CCGG 1 cut(s) 632
MspR9I CCNGG 2 cut(s) 633, 671
MunI CAATTG 1 cut(s) 686
MvaI CCWGG 1 cut(s) 671
NciI CCSGG 1 cut(s) 633
NcoI CCATGG 1 cut(s) 654
NdeI CATATG 1 cut(s) 1185
NdeII GATC 5 cut(s) 33, 142, 189, 552, 643
NlaIII CATG 9 cut(s) 221, 619, 658, 743, 757, 779, 955, 1003, 1225
NlaIV GGNNCC 2 cut(s) 233, 926
NspI RCATGY 1 cut(s) 221
PfeI GAWTC 1 cut(s) 253
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
PshBI ATTAAT 1 cut(s) 627
Psp6I CCWGG 1 cut(s) 669
PspFI CCCAGC 2 cut(s) 209, 234
PspGI CCWGG 1 cut(s) 669
PspN4I GGNNCC 2 cut(s) 233, 926
PspPI GGNCC 3 cut(s) 231, 331, 1106
PstI CTGCAG 2 cut(s) 529, 889
PstNI CAGNNNCTG 2 cut(s) 917, 1212
PsuI RGATCY 1 cut(s) 33
PvuII CAGCTG 1 cut(s) 1212
RsaI GTAC 1 cut(s) 1081
RsaNI GTAC 1 cut(s) 1080
RseI CAYNNNNRTG 6 cut(s) 69, 620, 780, 956, 1004, 1101
SaqAI TTAA 2 cut(s) 21, 627
Sau3AI GATC 5 cut(s) 33, 142, 189, 552, 643
Sau96I GGNCC 3 cut(s) 231, 331, 1106
ScaI AGTACT 1 cut(s) 1081
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 2 cut(s) 633, 671
SfaNI GCATC 4 cut(s) 127, 294, 481, 487
SfcI CTRYAG 2 cut(s) 525, 885
SinI GGWCC 1 cut(s) 231
SmiMI CAYNNNNRTG 6 cut(s) 69, 620, 780, 956, 1004, 1101
SmlI CTYRAG 3 cut(s) 29, 879, 906
SmoI CTYRAG 3 cut(s) 29, 879, 906
Sse9I AATT 7 cut(s) 108, 159, 197, 373, 686, 706, 760
SsiI CCGC 4 cut(s) 521, 636, 944, 1161
SspMI CTAG 4 cut(s) 842, 930, 1077, 1116
StyD4I CCNGG 2 cut(s) 631, 669
StyI CCWWGG 1 cut(s) 654
TaaI ACNGT 3 cut(s) 68, 977, 1139
TasI AATT 7 cut(s) 108, 159, 197, 373, 686, 706, 760
TatI WGTACW 1 cut(s) 1079
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 1 cut(s) 253
Tru1I TTAA 2 cut(s) 21, 627
Tru9I TTAA 2 cut(s) 21, 627
TscAI CASTG 5 cut(s) 71, 669, 754, 898, 1144
TspDTI ATGAA 8 cut(s) 27, 444, 632, 764, 773, 974, 1010, 1016
TspGWI ACGGA 1 cut(s) 1116
TspRI CASTG 5 cut(s) 71, 669, 754, 898, 1144
VpaK11BI GGWCC 1 cut(s) 231
VspI ATTAAT 1 cut(s) 627
XapI RAATTY 1 cut(s) 760
XceI RCATGY 1 cut(s) 221
XcmI CCANNNNNNNNNTGG 1 cut(s) 760
XspI CTAG 4 cut(s) 842, 930, 1077, 1116
ZrmI AGTACT 1 cut(s) 1081
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.