Rh4BG395700
ERF Family

heavy metal transport detoxification superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004807
Physical Location & Seq
Forward (+)
54697358 .. 54699609
2252 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG395700.1

Sequence Viewer

Length: 1242 bp
ATGACTAAAGATGAAGACTTTAAGCTCCTCAAGATCCAGACTTGTGTTCTCAAAGTGAACATACACTGTGATGGGTGTAAGCAGAAAGTGAAGAAACTGCTTCAGAGAATTGAAGGTGTTTACCAAGTCAGCATTGATGCTGATCAGCAGAAAGTAACAATTTCAGGGGTTATAGACTCTGCCACTTTGATCAAGAAATTGGTTAGAGCTGGGAAACATGCTGAGCTTTGGTCCCAGCAGAAGGCCAATCAGAACCAAAACCAGAAGCAAAAGAACACCAACTGCATCAAGGACAACAAAGGCCAAAAGCAGCAGGGGGGGATAATAAAGGGCCTTGAAGCCTTCAAAAACCAGCAGCAGCAGAAGTTCCCCAATTTCCTTATTGAAGAGGATATTGATGACTTCTTGGACGAGGAGGAGGAGGAGGATGAAGATGATGAGTTGAGGTTTCTGAGGCAGAAAGTGAACCAGCTGAGGCATCAAGCAATTGATGCAAACAACAATGCAAAGAAAGGTGTTGCGGCTGCAGCTAATCCCAACAGTAGTGTCAAGATCACAAACAATCACAATGCTGGAAAGAAGGGAGTGCAGCACCCAAATCAGAATGTGCAGGGCATGAAGGTGATTAATCCAGGCGGGATTGATCCCAAAACCATGGCAGCACTGAACCTGGGCAACATTGCTCAATTGGGTGGTGGAAACATCAATTTGGGTGGGGAAGTCAAGAGGGGGAATAACCATGACCTCAGTGCCATGATGAATTTGGCTGGCTTTCATGGCAATGGTGCCGCAAATGCTGCTACTTCTGCTGCTGGTATGGGTGGCAATCCCAATGTTAGTCTAGCAGCATTGCAACAAGCGCTTCATTCTCAGCAAGCTCAAGCTGCAGGCAGTGGATATCAGGCTCAAGCAGCTTCTGGAGGGTTCCCTAGTGGTGGTTATGCGACGGGCCAATATCCGCAGACCATGATGATGAACACAAATGGCTACGGGCATCCATCGCAGATGATGAACATGAATATGCAGGCGAGGCAAGCAATGCAGCAGCAGCAACCTCAGATGATGTATCATAGGTCTCCATTCAGTCCTCCTAGTACTACTGGTTATTACCACAACTATGGTCCTGCTCCTAGTTCTGTTCCATATCCATACAGTGTTGAGCCTAATTACAGCAGCGGTGATGGCAACAATGCAGCTCATATGTTCAATGATGAGAACACCAGCAGCTGTTCTATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

413

Amino Acids

44.81

Weight (kDa)

8.41

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 17 - 73 6.6e-15 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 785
AciI CCGC 5 cut(s) 521, 636, 789, 959, 1176
AclWI GGATC 2 cut(s) 28, 638
AcsI RAATTY 1 cut(s) 760
AcuI CTGAAG 1 cut(s) 86
AfaI GTAC 1 cut(s) 1096
AfeI AGCGCT 1 cut(s) 861
AfiI CCNNNNNNNGG 2 cut(s) 241, 935
AgsI TTSAA 5 cut(s) 113, 338, 346, 386, 1207
AjnI CCWGG 2 cut(s) 631, 669
Alw26I GTCTC 1 cut(s) 1080
AlwI GGATC 2 cut(s) 28, 638
AlwNI CAGNNNCTG 2 cut(s) 917, 1227
Aor51HI AGCGCT 1 cut(s) 861
AoxI GGCC 4 cut(s) 243, 301, 331, 949
ApoI RAATTY 1 cut(s) 760
AseI ATTAAT 1 cut(s) 627
AspLEI GCGC 1 cut(s) 862
AspS9I GGNCC 4 cut(s) 231, 331, 949, 1121
AsuHPI GGTGA 2 cut(s) 634, 1190
AvaII GGWCC 2 cut(s) 231, 1121
BanI GGYRCC 1 cut(s) 785
BbsI GAAGAC 1 cut(s) 21
BbvCI CCTCAGC 1 cut(s) 473
BccI CCATC 3 cut(s) 65, 1006, 1175
BciT130I CCWGG 2 cut(s) 633, 671
BclI TGATCA 2 cut(s) 142, 189
BcoDI GTCTC 1 cut(s) 1080
BfaI CTAG 4 cut(s) 842, 930, 1092, 1131
BfmI CTRYAG 2 cut(s) 525, 885
BfoI RGCGCY 1 cut(s) 863
BlpI GCTNAGC 1 cut(s) 222
BmcAI AGTACT 1 cut(s) 1096
Bme1390I CCNGG 2 cut(s) 633, 671
Bme18I GGWCC 2 cut(s) 231, 1121
BmgT120I GGNCC 4 cut(s) 231, 331, 949, 1121
BmiI GGNNCC 3 cut(s) 233, 787, 926
BmrFI CCNGG 2 cut(s) 633, 671
BmsI GCATC 5 cut(s) 127, 294, 481, 487, 1003
BpiI GAAGAC 1 cut(s) 21
BpmI CTGGAG 1 cut(s) 939
Bpu10I CCTNAGC 1 cut(s) 473
Bpu1102I GCTNAGC 1 cut(s) 222
BpuEI CTTGAG 3 cut(s) 14, 864, 891
BsaBI GATNNNNATC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 1080
BsaJI CCNNGG 2 cut(s) 654, 670
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 935
Bse1I ACTGG 1 cut(s) 1105
Bse3DI GCAATG 4 cut(s) 678, 787, 848, 1044
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 2 cut(s) 633, 671
BseDI CCNNGG 2 cut(s) 654, 670
BseGI GGATG 2 cut(s) 433, 994
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 2 cut(s) 241, 935
BseMI GCAATG 4 cut(s) 678, 787, 848, 1044
BseMII CTCAG 6 cut(s) 213, 443, 464, 760, 884, 1070
BseNI ACTGG 1 cut(s) 1105
BseRI GAGGAG 5 cut(s) 17, 428, 431, 434, 437
BseYI CCCAGC 2 cut(s) 209, 234
BsgI GTGCAG 2 cut(s) 608, 629
BshFI GGCC 4 cut(s) 245, 303, 333, 951
BshNI GGYRCC 1 cut(s) 785
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 2 cut(s) 241, 935
BsmAI GTCTC 1 cut(s) 1080
BsmFI GGGAC 1 cut(s) 217
BsnI GGCC 4 cut(s) 245, 303, 333, 951
Bso31I GGTCTC 1 cut(s) 1080
Bsp143I GATC 5 cut(s) 33, 142, 189, 552, 643
Bsp1720I GCTNAGC 1 cut(s) 222
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 5 cut(s) 521, 636, 789, 959, 1176
BspANI GGCC 4 cut(s) 245, 303, 333, 951
BspCNI CTCAG 6 cut(s) 214, 444, 465, 759, 883, 1069
BspLI GGNNCC 3 cut(s) 233, 787, 926
BspMAI CTGCAG 2 cut(s) 529, 889
BspPI GGATC 2 cut(s) 28, 638
BspT107I GGYRCC 1 cut(s) 785
BspTNI GGTCTC 1 cut(s) 1080
BsrDI GCAATG 4 cut(s) 678, 787, 848, 1044
BsrI ACTGG 1 cut(s) 1105
BssECI CCNNGG 2 cut(s) 654, 670
BssMI GATC 5 cut(s) 33, 142, 189, 552, 643
BssT1I CCWWGG 1 cut(s) 654
Bst2UI CCWGG 2 cut(s) 633, 671
Bst4CI ACNGT 3 cut(s) 68, 542, 1154
Bst6I CTCTTC 1 cut(s) 381
BstAPI GCANNNNNTGC 3 cut(s) 491, 797, 1039
BstC8I GCNNGC 5 cut(s) 769, 876, 889, 1026, 1035
BstDEI CTNAG 6 cut(s) 222, 452, 473, 746, 870, 1056
BstDSI CCRYGG 1 cut(s) 654
BstF5I GGATG 2 cut(s) 433, 994
BstH2I RGCGCY 1 cut(s) 863
BstHHI GCGC 1 cut(s) 862
BstKTI GATC 5 cut(s) 36, 145, 192, 555, 646
BstMAI GTCTC 1 cut(s) 1080
BstMBI GATC 5 cut(s) 33, 142, 189, 552, 643
BstNI CCWGG 2 cut(s) 633, 671
BstNSI RCATGY 1 cut(s) 221
BstSCI CCNGG 2 cut(s) 631, 669
BstSFI CTRYAG 2 cut(s) 525, 885
BstV2I GAAGAC 1 cut(s) 21
BstX2I RGATCY 1 cut(s) 33
BstXI CCANNNNNNTGG 2 cut(s) 655, 1118
BstYI RGATCY 1 cut(s) 33
BsuRI GGCC 4 cut(s) 245, 303, 333, 951
BtgI CCRYGG 1 cut(s) 654
BtgZI GCGATG 1 cut(s) 984
BtsCI GGATG 2 cut(s) 433, 994
BtsI GCAGTG 1 cut(s) 898
BtsIMutI CAGTG 5 cut(s) 64, 662, 754, 898, 1159
Cac8I GCNNGC 5 cut(s) 769, 876, 889, 1026, 1035
CaiI CAGNNNCTG 2 cut(s) 917, 1227
CfoI GCGC 1 cut(s) 862
Cfr13I GGNCC 4 cut(s) 231, 331, 949, 1121
Csp6I GTAC 1 cut(s) 1095
CspCI CAANNNNNGTGG 2 cut(s) 694, 729
CviAII CATG 9 cut(s) 218, 616, 655, 740, 754, 776, 967, 1015, 1237
CviQI GTAC 1 cut(s) 1095
DdeI CTNAG 6 cut(s) 222, 452, 473, 746, 870, 1056
DpnI GATC 5 cut(s) 35, 144, 191, 554, 645
DpnII GATC 5 cut(s) 33, 142, 189, 552, 643
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
Eco130I CCWWGG 1 cut(s) 654
Eco31I GGTCTC 1 cut(s) 1080
Eco32I GATATC 1 cut(s) 899
Eco47I GGWCC 2 cut(s) 231, 1121
Eco47III AGCGCT 1 cut(s) 861
Eco57I CTGAAG 1 cut(s) 86
EcoO109I RGGNCCY 1 cut(s) 331
EcoRII CCWGG 2 cut(s) 631, 669
EcoRV GATATC 1 cut(s) 899
EcoT14I CCWWGG 1 cut(s) 654
ErhI CCWWGG 1 cut(s) 654
FaeI CATG 9 cut(s) 221, 619, 658, 743, 757, 779, 970, 1018, 1240
FaqI GGGAC 1 cut(s) 217
FatI CATG 9 cut(s) 217, 615, 654, 739, 753, 775, 966, 1014, 1236
FauI CCCGC 1 cut(s) 629
FauNDI CATATG 1 cut(s) 1200
FbaI TGATCA 2 cut(s) 142, 189
FokI GGATG 2 cut(s) 440, 981
FspBI CTAG 4 cut(s) 842, 930, 1092, 1131
GlaI GCGC 1 cut(s) 861
GsaI CCCAGC 2 cut(s) 213, 238
GsuI CTGGAG 1 cut(s) 939
HaeII RGCGCY 1 cut(s) 863
HaeIII GGCC 4 cut(s) 245, 303, 333, 951
HhaI GCGC 1 cut(s) 862
Hin1II CATG 9 cut(s) 221, 619, 658, 743, 757, 779, 970, 1018, 1240
Hin6I GCGC 1 cut(s) 860
HinP1I GCGC 1 cut(s) 860
HinfI GANTC 1 cut(s) 176
HphI GGTGA 2 cut(s) 634, 1190
Hpy166II GTNNAC 3 cut(s) 58, 121, 466
Hpy188I TCNGA 5 cut(s) 105, 252, 453, 603, 1059
Hpy188III TCNNGA 6 cut(s) 31, 37, 193, 550, 724, 918
Hpy8I GTNNAC 3 cut(s) 58, 121, 466
Hpy99I CGWCG 1 cut(s) 949
HpyAV CCTTC 5 cut(s) 107, 235, 352, 574, 613
HpyCH4III ACNGT 3 cut(s) 68, 542, 1154
HpyF3I CTNAG 6 cut(s) 222, 452, 473, 746, 870, 1056
Hsp92II CATG 9 cut(s) 221, 619, 658, 743, 757, 779, 970, 1018, 1240
HspAI GCGC 1 cut(s) 860
Ksp22I TGATCA 2 cut(s) 142, 189
Kzo9I GATC 5 cut(s) 33, 142, 189, 552, 643
LmnI GCTCC 2 cut(s) 30, 1132
LweI GCATC 5 cut(s) 127, 294, 481, 487, 1003
MaeI CTAG 4 cut(s) 842, 930, 1092, 1131
MaeIII GTNAC 1 cut(s) 154
MalI GATC 5 cut(s) 35, 144, 191, 554, 645
MboI GATC 5 cut(s) 33, 142, 189, 552, 643
MboII GAAGA 4 cut(s) 26, 103, 398, 443
MfeI CAATTG 2 cut(s) 486, 686
MflI RGATCY 1 cut(s) 33
MluCI AATT 9 cut(s) 108, 159, 197, 373, 486, 686, 706, 760, 1165
MlyI GAGTC 1 cut(s) 170
MseI TTAA 2 cut(s) 21, 627
MslI CAYNNNNRTG 6 cut(s) 69, 620, 780, 971, 1019, 1116
MspA1I CMGCKG 3 cut(s) 472, 1176, 1227
MspR9I CCNGG 2 cut(s) 633, 671
MunI CAATTG 2 cut(s) 486, 686
MvaI CCWGG 2 cut(s) 633, 671
NcoI CCATGG 1 cut(s) 654
NdeI CATATG 1 cut(s) 1200
NdeII GATC 5 cut(s) 33, 142, 189, 552, 643
NlaIII CATG 9 cut(s) 221, 619, 658, 743, 757, 779, 970, 1018, 1240
NlaIV GGNNCC 3 cut(s) 233, 787, 926
NspI RCATGY 1 cut(s) 221
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
PshBI ATTAAT 1 cut(s) 627
Psp6I CCWGG 2 cut(s) 631, 669
PspFI CCCAGC 2 cut(s) 209, 234
PspGI CCWGG 2 cut(s) 631, 669
PspN4I GGNNCC 3 cut(s) 233, 787, 926
PspPI GGNCC 4 cut(s) 231, 331, 949, 1121
PstI CTGCAG 2 cut(s) 529, 889
PstNI CAGNNNCTG 2 cut(s) 917, 1227
PsuI RGATCY 1 cut(s) 33
PvuII CAGCTG 2 cut(s) 472, 1227
RsaI GTAC 1 cut(s) 1096
RsaNI GTAC 1 cut(s) 1095
RseI CAYNNNNRTG 6 cut(s) 69, 620, 780, 971, 1019, 1116
SaqAI TTAA 2 cut(s) 21, 627
Sau3AI GATC 5 cut(s) 33, 142, 189, 552, 643
Sau96I GGNCC 4 cut(s) 231, 331, 949, 1121
ScaI AGTACT 1 cut(s) 1096
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 2 cut(s) 633, 671
SfaNI GCATC 5 cut(s) 127, 294, 481, 487, 1003
SfcI CTRYAG 2 cut(s) 525, 885
SinI GGWCC 2 cut(s) 231, 1121
SmiMI CAYNNNNRTG 6 cut(s) 69, 620, 780, 971, 1019, 1116
SmlI CTYRAG 3 cut(s) 29, 879, 906
SmoI CTYRAG 3 cut(s) 29, 879, 906
Sse9I AATT 9 cut(s) 108, 159, 197, 373, 486, 686, 706, 760, 1165
SsiI CCGC 5 cut(s) 521, 636, 789, 959, 1176
SspMI CTAG 4 cut(s) 842, 930, 1092, 1131
StyD4I CCNGG 2 cut(s) 631, 669
StyI CCWWGG 1 cut(s) 654
TaaI ACNGT 3 cut(s) 68, 542, 1154
TasI AATT 9 cut(s) 108, 159, 197, 373, 486, 686, 706, 760, 1165
TatI WGTACW 1 cut(s) 1094
TauI GCSGC 2 cut(s) 524, 791
Tru1I TTAA 2 cut(s) 21, 627
Tru9I TTAA 2 cut(s) 21, 627
TscAI CASTG 5 cut(s) 71, 669, 754, 898, 1159
TspDTI ATGAA 9 cut(s) 27, 444, 632, 764, 773, 854, 989, 1025, 1031
TspRI CASTG 5 cut(s) 71, 669, 754, 898, 1159
VpaK11BI GGWCC 2 cut(s) 231, 1121
VspI ATTAAT 1 cut(s) 627
XapI RAATTY 1 cut(s) 760
XceI RCATGY 1 cut(s) 221
XcmI CCANNNNNNNNNTGG 1 cut(s) 760
XspI CTAG 4 cut(s) 842, 930, 1092, 1131
ZrmI AGTACT 1 cut(s) 1096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.