Rh4DG388800
ERF Family

heavy metal transport detoxification superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004807
Physical Location & Seq
Forward (+)
60526872 .. 60529663
2792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG388800.1

Sequence Viewer

Length: 1248 bp
ATGACTAAAGATGAAGACTTTAAGCTTCTCAAGATCCAGACTTGTGTTCTCAAAGTGAACATACACTGTGATGGGTGTAAGCAGAAAGTGAAGAAACTGCTTCAGAGAATTGAAGGTGTTTACCAAGTCAGCATTGATGCTGATCAGCAGAAAGTAACAATTTCAGGGGTTATAGACGCTGCCACTTTGATCAAGAAATTGGTTAGAGCTGGGAAACATGCTGAGCTTTGGTCCCAGCAGAAGGCCAATCAGAACCAAAACCAGAAGCAAAAGAACACCAACTGCATCAAGGACAACAAAGGCCAAAAGCAGCAGGGGGGGATAATAAAGGGCCTTGAAGCCTTCAAAAACCAGCAGCAGCAGCAGAAGTTCCCCAATTTCCTTATTGAAGAGGATATTGATGACTTCTTGGACGGCGATGAGGAGGAGGATGAAGATGATGAGTTGAGGTTTCTGAGGCAGAAAGTGAACCAGCTCAGGCATCAAGCGATTGATGCAAACAACAATGCAAAGAAAGGTGTTGCGGCTGCAGCTAATCCCAACAATAGTGTCAAGATCACAAACAATCACAATGCTGGAAAGAAGGGAGTGCAGCACCCAAATCAGAATGTGCAGGGCATGAAGGTGATTAATCCAGGCGGGATTGATCCCAAAACCATGGCAGCACTGAACCTGGGCAACATTGCTCAATTGGGTGGTGGAAACATCAATTTGGGTGGGGAAGTCAAGAGGGGGAATAACCATGACCTCAGTGCCATGATGAATTTGGCTGGCTTTCATGGCAATGGTGCTGCAAATGCTGCTACTTCTGCTGCTGGTATGGGTAGCAATCCCAATGTTAGTCTAGCAGCATTGCAACAAGCGCTTCAATCTCAGCAAGCTCAAGCTGCAGGCAGTGGATATCAGGCTCAAGCAGCTTCTGGAGGGTTCCCTAGTGGTGGTTATGCGACGGGCCAATATCCGCAGACCATGATGATGAACACAAATGGCTACGGGCATCCATCGCAGATGATGAACATGAATATGCAGGCGAGGCAAGCAATGCAGCAGCAGCAGCAACCTCAGATGATGTATCATAGGTCTCCATTCAGTCCTCCTAGTACTACTGGTTATTACCACAACTATGGTCCTATTCCTAGTTCTGTTCCATATCCATACAGTGCTGAGCCTAATTACAGCAACGGTGATGGCAACAATGCAGCTCATATGTTCAATGATGAGAACACCAGCAGCTGTTCTATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

415

Amino Acids

45.05

Weight (kDa)

8.6

Isoelectric Point (pI)

38.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 17 - 73 1.2e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 524, 639, 962
AclWI GGATC 2 cut(s) 28, 641
AcsI RAATTY 1 cut(s) 763
AcuI CTGAAG 1 cut(s) 86
AfaI GTAC 1 cut(s) 1102
AfeI AGCGCT 1 cut(s) 864
AfiI CCNNNNNNNGG 2 cut(s) 241, 938
AgsI TTSAA 6 cut(s) 113, 338, 346, 389, 869, 1213
AjnI CCWGG 2 cut(s) 634, 672
Alw26I GTCTC 1 cut(s) 1086
AlwI GGATC 2 cut(s) 28, 641
AlwNI CAGNNNCTG 2 cut(s) 920, 1233
Aor51HI AGCGCT 1 cut(s) 864
AoxI GGCC 4 cut(s) 243, 301, 331, 952
ApoI RAATTY 1 cut(s) 763
AseI ATTAAT 1 cut(s) 630
AspLEI GCGC 1 cut(s) 865
AspS9I GGNCC 4 cut(s) 231, 331, 952, 1127
AsuHPI GGTGA 2 cut(s) 637, 1196
AvaII GGWCC 2 cut(s) 231, 1127
BbsI GAAGAC 1 cut(s) 21
BccI CCATC 3 cut(s) 65, 1009, 1181
BceAI ACGGC 1 cut(s) 430
BciT130I CCWGG 2 cut(s) 636, 674
BclI TGATCA 2 cut(s) 142, 189
BcoDI GTCTC 1 cut(s) 1086
BfaI CTAG 4 cut(s) 845, 933, 1098, 1137
BfmI CTRYAG 2 cut(s) 528, 888
BfoI RGCGCY 1 cut(s) 866
BlpI GCTNAGC 2 cut(s) 222, 1164
BmcAI AGTACT 1 cut(s) 1102
Bme1390I CCNGG 2 cut(s) 636, 674
Bme18I GGWCC 2 cut(s) 231, 1127
BmgT120I GGNCC 4 cut(s) 231, 331, 952, 1127
BmiI GGNNCC 2 cut(s) 233, 929
BmrFI CCNGG 2 cut(s) 636, 674
BmsI GCATC 5 cut(s) 127, 294, 484, 490, 1006
BpiI GAAGAC 1 cut(s) 21
BpmI CTGGAG 1 cut(s) 942
Bpu10I CCTNAGC 1 cut(s) 476
Bpu1102I GCTNAGC 2 cut(s) 222, 1164
BpuEI CTTGAG 3 cut(s) 14, 867, 894
BsaBI GATNNNNATC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 1086
BsaJI CCNNGG 2 cut(s) 657, 673
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 938
Bse1I ACTGG 1 cut(s) 1111
Bse3DI GCAATG 4 cut(s) 681, 790, 851, 1047
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 2 cut(s) 636, 674
BseDI CCNNGG 2 cut(s) 657, 673
BseGI GGATG 2 cut(s) 436, 997
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 2 cut(s) 241, 938
BseMI GCAATG 4 cut(s) 681, 790, 851, 1047
BseMII CTCAG 7 cut(s) 213, 446, 490, 763, 887, 1076, 1155
BseNI ACTGG 1 cut(s) 1111
BseRI GAGGAG 2 cut(s) 437, 440
BseYI CCCAGC 2 cut(s) 209, 234
BsgI GTGCAG 2 cut(s) 611, 632
BshFI GGCC 4 cut(s) 245, 303, 333, 954
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 2 cut(s) 241, 938
BsmAI GTCTC 1 cut(s) 1086
BsmFI GGGAC 1 cut(s) 217
BsnI GGCC 4 cut(s) 245, 303, 333, 954
Bso31I GGTCTC 1 cut(s) 1086
Bsp143I GATC 5 cut(s) 33, 142, 189, 555, 646
Bsp1720I GCTNAGC 2 cut(s) 222, 1164
Bsp19I CCATGG 1 cut(s) 657
BspACI CCGC 3 cut(s) 524, 639, 962
BspANI GGCC 4 cut(s) 245, 303, 333, 954
BspCNI CTCAG 7 cut(s) 214, 447, 489, 762, 886, 1075, 1156
BspLI GGNNCC 2 cut(s) 233, 929
BspMAI CTGCAG 2 cut(s) 532, 892
BspPI GGATC 2 cut(s) 28, 641
BspTNI GGTCTC 1 cut(s) 1086
BsrDI GCAATG 4 cut(s) 681, 790, 851, 1047
BsrI ACTGG 1 cut(s) 1111
BssECI CCNNGG 2 cut(s) 657, 673
BssMI GATC 5 cut(s) 33, 142, 189, 555, 646
BssT1I CCWWGG 1 cut(s) 657
Bst2UI CCWGG 2 cut(s) 636, 674
Bst4CI ACNGT 3 cut(s) 68, 1160, 1184
Bst6I CTCTTC 1 cut(s) 384
BstAPI GCANNNNNTGC 2 cut(s) 800, 1042
BstC8I GCNNGC 5 cut(s) 772, 879, 892, 1029, 1038
BstDEI CTNAG 7 cut(s) 222, 455, 476, 749, 873, 1062, 1164
BstDSI CCRYGG 1 cut(s) 657
BstF5I GGATG 2 cut(s) 436, 997
BstH2I RGCGCY 1 cut(s) 866
BstHHI GCGC 1 cut(s) 865
BstKTI GATC 5 cut(s) 36, 145, 192, 558, 649
BstMAI GTCTC 1 cut(s) 1086
BstMBI GATC 5 cut(s) 33, 142, 189, 555, 646
BstNI CCWGG 2 cut(s) 636, 674
BstNSI RCATGY 1 cut(s) 221
BstSCI CCNGG 2 cut(s) 634, 672
BstSFI CTRYAG 2 cut(s) 528, 888
BstV2I GAAGAC 1 cut(s) 21
BstX2I RGATCY 1 cut(s) 33
BstXI CCANNNNNNTGG 2 cut(s) 658, 1124
BstYI RGATCY 1 cut(s) 33
BsuRI GGCC 4 cut(s) 245, 303, 333, 954
BtgI CCRYGG 1 cut(s) 657
BtgZI GCGATG 2 cut(s) 432, 987
BtsCI GGATG 2 cut(s) 436, 997
BtsI GCAGTG 1 cut(s) 901
BtsIMutI CAGTG 5 cut(s) 64, 665, 757, 901, 1165
Cac8I GCNNGC 5 cut(s) 772, 879, 892, 1029, 1038
CaiI CAGNNNCTG 2 cut(s) 920, 1233
CfoI GCGC 1 cut(s) 865
Cfr13I GGNCC 4 cut(s) 231, 331, 952, 1127
CseI GACGC 1 cut(s) 185
Csp6I GTAC 1 cut(s) 1101
CspCI CAANNNNNGTGG 2 cut(s) 697, 732
CviAII CATG 9 cut(s) 218, 619, 658, 743, 757, 779, 970, 1018, 1243
CviQI GTAC 1 cut(s) 1101
DdeI CTNAG 7 cut(s) 222, 455, 476, 749, 873, 1062, 1164
DpnI GATC 5 cut(s) 35, 144, 191, 557, 648
DpnII GATC 5 cut(s) 33, 142, 189, 555, 646
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
Eco130I CCWWGG 1 cut(s) 657
Eco31I GGTCTC 1 cut(s) 1086
Eco32I GATATC 1 cut(s) 902
Eco47I GGWCC 2 cut(s) 231, 1127
Eco47III AGCGCT 1 cut(s) 864
Eco57I CTGAAG 1 cut(s) 86
EcoO109I RGGNCCY 1 cut(s) 331
EcoRII CCWGG 2 cut(s) 634, 672
EcoRV GATATC 1 cut(s) 902
EcoT14I CCWWGG 1 cut(s) 657
ErhI CCWWGG 1 cut(s) 657
FaeI CATG 9 cut(s) 221, 622, 661, 746, 760, 782, 973, 1021, 1246
FaqI GGGAC 1 cut(s) 217
FatI CATG 9 cut(s) 217, 618, 657, 742, 756, 778, 969, 1017, 1242
FauI CCCGC 1 cut(s) 632
FauNDI CATATG 1 cut(s) 1206
FbaI TGATCA 2 cut(s) 142, 189
FokI GGATG 2 cut(s) 443, 984
FspBI CTAG 4 cut(s) 845, 933, 1098, 1137
GlaI GCGC 1 cut(s) 864
GsaI CCCAGC 2 cut(s) 213, 238
GsuI CTGGAG 1 cut(s) 942
HaeII RGCGCY 1 cut(s) 866
HaeIII GGCC 4 cut(s) 245, 303, 333, 954
HgaI GACGC 1 cut(s) 185
HhaI GCGC 1 cut(s) 865
Hin1II CATG 9 cut(s) 221, 622, 661, 746, 760, 782, 973, 1021, 1246
Hin6I GCGC 1 cut(s) 863
HinP1I GCGC 1 cut(s) 863
HindIII AAGCTT 1 cut(s) 23
HphI GGTGA 2 cut(s) 637, 1196
Hpy166II GTNNAC 3 cut(s) 58, 121, 469
Hpy188I TCNGA 5 cut(s) 105, 252, 456, 606, 1065
Hpy188III TCNNGA 6 cut(s) 31, 37, 193, 553, 727, 921
Hpy8I GTNNAC 3 cut(s) 58, 121, 469
Hpy99I CGWCG 1 cut(s) 952
HpyAV CCTTC 5 cut(s) 107, 235, 352, 577, 616
HpyCH4III ACNGT 3 cut(s) 68, 1160, 1184
HpyF3I CTNAG 7 cut(s) 222, 455, 476, 749, 873, 1062, 1164
Hsp92II CATG 9 cut(s) 221, 622, 661, 746, 760, 782, 973, 1021, 1246
HspAI GCGC 1 cut(s) 863
Ksp22I TGATCA 2 cut(s) 142, 189
Kzo9I GATC 5 cut(s) 33, 142, 189, 555, 646
LweI GCATC 5 cut(s) 127, 294, 484, 490, 1006
MaeI CTAG 4 cut(s) 845, 933, 1098, 1137
MaeIII GTNAC 1 cut(s) 154
MalI GATC 5 cut(s) 35, 144, 191, 557, 648
MboI GATC 5 cut(s) 33, 142, 189, 555, 646
MboII GAAGA 4 cut(s) 26, 103, 401, 446
MfeI CAATTG 1 cut(s) 689
MflI RGATCY 1 cut(s) 33
MluCI AATT 8 cut(s) 108, 159, 197, 376, 689, 709, 763, 1171
MseI TTAA 2 cut(s) 21, 630
MslI CAYNNNNRTG 6 cut(s) 69, 623, 783, 974, 1022, 1122
MspA1I CMGCKG 1 cut(s) 1233
MspR9I CCNGG 2 cut(s) 636, 674
MunI CAATTG 1 cut(s) 689
MvaI CCWGG 2 cut(s) 636, 674
NcoI CCATGG 1 cut(s) 657
NdeI CATATG 1 cut(s) 1206
NdeII GATC 5 cut(s) 33, 142, 189, 555, 646
NlaIII CATG 9 cut(s) 221, 622, 661, 746, 760, 782, 973, 1021, 1246
NlaIV GGNNCC 2 cut(s) 233, 929
NspI RCATGY 1 cut(s) 221
PshBI ATTAAT 1 cut(s) 630
Psp6I CCWGG 2 cut(s) 634, 672
PspFI CCCAGC 2 cut(s) 209, 234
PspGI CCWGG 2 cut(s) 634, 672
PspN4I GGNNCC 2 cut(s) 233, 929
PspPI GGNCC 4 cut(s) 231, 331, 952, 1127
PstI CTGCAG 2 cut(s) 532, 892
PstNI CAGNNNCTG 2 cut(s) 920, 1233
PsuI RGATCY 1 cut(s) 33
PvuII CAGCTG 1 cut(s) 1233
RsaI GTAC 1 cut(s) 1102
RsaNI GTAC 1 cut(s) 1101
RseI CAYNNNNRTG 6 cut(s) 69, 623, 783, 974, 1022, 1122
SaqAI TTAA 2 cut(s) 21, 630
Sau3AI GATC 5 cut(s) 33, 142, 189, 555, 646
Sau96I GGNCC 4 cut(s) 231, 331, 952, 1127
ScaI AGTACT 1 cut(s) 1102
ScrFI CCNGG 2 cut(s) 636, 674
SfaNI GCATC 5 cut(s) 127, 294, 484, 490, 1006
SfcI CTRYAG 2 cut(s) 528, 888
SinI GGWCC 2 cut(s) 231, 1127
SmiMI CAYNNNNRTG 6 cut(s) 69, 623, 783, 974, 1022, 1122
SmlI CTYRAG 3 cut(s) 29, 882, 909
SmoI CTYRAG 3 cut(s) 29, 882, 909
Sse9I AATT 8 cut(s) 108, 159, 197, 376, 689, 709, 763, 1171
SsiI CCGC 3 cut(s) 524, 639, 962
SspMI CTAG 4 cut(s) 845, 933, 1098, 1137
StyD4I CCNGG 2 cut(s) 634, 672
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 3 cut(s) 68, 1160, 1184
TasI AATT 8 cut(s) 108, 159, 197, 376, 689, 709, 763, 1171
TatI WGTACW 1 cut(s) 1100
TauI GCSGC 1 cut(s) 527
Tru1I TTAA 2 cut(s) 21, 630
Tru9I TTAA 2 cut(s) 21, 630
TscAI CASTG 5 cut(s) 71, 672, 757, 901, 1165
TspDTI ATGAA 8 cut(s) 27, 447, 635, 767, 776, 992, 1028, 1034
TspRI CASTG 5 cut(s) 71, 672, 757, 901, 1165
VpaK11BI GGWCC 2 cut(s) 231, 1127
VspI ATTAAT 1 cut(s) 630
XapI RAATTY 1 cut(s) 763
XceI RCATGY 1 cut(s) 221
XcmI CCANNNNNNNNNTGG 1 cut(s) 763
XspI CTAG 4 cut(s) 845, 933, 1098, 1137
ZrmI AGTACT 1 cut(s) 1102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.