FvH4_4g33180

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004540
Physical Location & Seq
Reverse (-)
31482026 .. 31484041
2016 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33180.t1

Sequence Viewer

Length: 1320 bp
ATGTCTTCCCACAAAGTTGCCTTTCAGGCTTGCTTCTTGTCCAAACAATGGGAAGGTGTAATATCCTCCTTCCCTGCACTAAATTTCGACGAGGATGATGATTTGGACCGCCATCGCCCCAACCTCAATGGCGAGAATGACTATGTACATCACAAAACGTTCATCAACTTCCTCAACGGGTATCTAGAACATCTTGACAACAGAAAGCACATAGACAAATTGAGGCTTCATACATGGGTGAGCACGTATCGAGATGTTGCAAACATCGACAGATGTTTAGAAGTTGCTATAGAGAGAGATGTGAAAGAGTTGGATATCAGCCCTATACTTGGCGGCGATCAGGTTGCGCTTAGTAAGCGAAGCTGGGTAAAGAGATACAAAAACTCCATATTTTACAGCATACCTTGGTTGACCCTTGTTAATGCAAAATCTTTAACTAGTCTAAATCTGGAGTGTGTGAGAGTTCCGGACCTTCTGGACCTTCTGGACCTTCTAGCTGGACCTGACCGCTCTCTGCTCCCCTCTCTAAAAACCATGTCTTTCACAGCTGTGAGGCTCCATGATTGTAGTTTCTTCCCTCTACTAAGGATATCCTCTTCCATCGAGTACTTGTCATTAACTGCATGTTCTTTCGAGGGCAATGATCAGTGTGACGTTTACTGCACGAGTCTCAAGAGCTTGGAAGTTAAGTATTGTCATGGGCTTAGGTCAATTATGGTCGGAGCTATGAATCTCGAATCTTTTACATTGGTTGGATATACAAATTGTTCATTTGTGGATGTGGATGATGCCCCCAACTTGAAAAAGATCGACATATGTGTTGATAAACTTAAGCAATTATATTTGTGGCGATGCCATCGTGCAGTAGAGGCCACCATCAATGTCCAAAGCCTCAGACTTTTCAAATTTTACGGTTCATTGGAAGCTCAGGTATCTGTTAAAGCATGTTGGAAATGCATAATTTGGGTTACTGAACTTTGGTTACAAGAATGGTTCTTGTCAAACTCGAAAAATTTGTTTACACTGATCGAGTTCCTTAAAGGATTTGGCTGCTGCAAGAAGGTCTGGTTATGTACTCGTGATTCGAAAGCTCTCATCATTCCGGAATCTTTCAGGAAGACCATAGGCACATTCCCTACACCGTTGCCTGAAAGTTGCACTCTGAATGTGGCAATGCCGAATCCTCCGAAACCGGGCAGTAGAGACTTCTTGGACTTGAATGACTCCATCAATTGGATTGCACATTCTGCACGGATTGTTAAACAAAATCCCCAATGGCTCATTGAGCTCTTCGCAAACTACACAAACGGATCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

440

Amino Acids

50.3

Weight (kDa)

6.82

Isoelectric Point (pI)

43.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 138 - 281 2.1e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 48, 1233
AccBSI CCGCTC 1 cut(s) 510
AccIII TCCGGA 2 cut(s) 466, 1102
AciI CCGC 3 cut(s) 109, 333, 508
AclI AACGTT 1 cut(s) 158
AclWI GGATC 1 cut(s) 1318
AcsI RAATTY 3 cut(s) 82, 905, 1012
AfaI GTAC 3 cut(s) 147, 608, 1075
AfiI CCNNNNNNNGG 4 cut(s) 48, 329, 1193, 1233
AflII CTTAAG 1 cut(s) 830
AgsI TTSAA 3 cut(s) 802, 904, 1219
AhlI ACTAGT 1 cut(s) 437
AleI CACNNNNGTG 1 cut(s) 548
AluBI AGCT 8 cut(s) 363, 497, 548, 678, 725, 926, 1091, 1288
AluI AGCT 8 cut(s) 363, 497, 548, 678, 725, 926, 1091, 1288
Alw21I GWGCWC 2 cut(s) 245, 1290
Alw26I GTCTC 2 cut(s) 674, 1197
AlwI GGATC 1 cut(s) 1318
Aor13HI TCCGGA 2 cut(s) 466, 1102
AoxI GGCC 1 cut(s) 870
ApeKI GCWGC 2 cut(s) 1050, 1053
ApoI RAATTY 3 cut(s) 82, 905, 1012
AspLEI GCGC 1 cut(s) 349
AspS9I GGNCC 5 cut(s) 106, 469, 478, 487, 500
AsuC2I CCSGG 1 cut(s) 1194
AsuHPI GGTGA 1 cut(s) 250
AsuII TTCGAA 1 cut(s) 1085
AvaII GGWCC 5 cut(s) 106, 469, 478, 487, 500
BanII GRGCYC 1 cut(s) 1290
BauI CACGAG 2 cut(s) 664, 1077
BbsI GAAGAC 1 cut(s) 1124
Bbv12I GWGCWC 2 cut(s) 245, 1290
BbvI GCAGC 2 cut(s) 1037, 1040
BccI CCATC 5 cut(s) 120, 608, 864, 884, 1235
BcgI CGANNNNNNTGC 2 cut(s) 326, 360
BclI TGATCA 1 cut(s) 643
BcnI CCSGG 1 cut(s) 1194
BcoDI GTCTC 2 cut(s) 674, 1197
BcuI ACTAGT 1 cut(s) 437
BfaI CTAG 3 cut(s) 185, 438, 494
BfmI CTRYAG 1 cut(s) 288
BfrI CTTAAG 1 cut(s) 830
BglI GCCNNNNNGGC 1 cut(s) 26
BisI GCNGC 3 cut(s) 334, 1051, 1054
BlsI GCNGC 3 cut(s) 335, 1052, 1055
BmcAI AGTACT 1 cut(s) 608
Bme1390I CCNGG 1 cut(s) 1194
Bme18I GGWCC 5 cut(s) 106, 469, 478, 487, 500
BmgT120I GGNCC 5 cut(s) 106, 469, 478, 487, 500
BmiI GGNNCC 1 cut(s) 557
BmrFI CCNGG 1 cut(s) 1194
BmsI GCATC 2 cut(s) 778, 842
BpiI GAAGAC 1 cut(s) 1124
BpmI CTGGAG 1 cut(s) 470
Bpu10I CCTNAGC 2 cut(s) 704, 927
Bpu14I TTCGAA 1 cut(s) 1085
BpuEI CTTGAG 1 cut(s) 656
BpuMI CCSGG 1 cut(s) 1194
BsaAI YACGTR 1 cut(s) 246
BsaJI CCNNGG 1 cut(s) 404
BsaWI WCCGGW 2 cut(s) 466, 1102
BsaXI ACNNNNNCTCC 2 cut(s) 368, 398
Bsc4I CCNNNNNNNGG 4 cut(s) 48, 329, 1193, 1233
Bse3DI GCAATG 2 cut(s) 646, 1179
BseAI TCCGGA 2 cut(s) 466, 1102
BseDI CCNNGG 1 cut(s) 404
BseGI GGATG 3 cut(s) 100, 784, 790
BseLI CCNNNNNNNGG 4 cut(s) 48, 329, 1193, 1233
BseMI GCAATG 2 cut(s) 646, 1179
BseMII CTCAG 2 cut(s) 907, 941
BseXI GCAGC 2 cut(s) 1037, 1040
BseYI CCCAGC 1 cut(s) 363
BsgI GTGCAG 4 cut(s) 60, 646, 882, 1233
BshFI GGCC 1 cut(s) 872
BsiHKAI GWGCWC 2 cut(s) 245, 1290
BsiSI CCGG 3 cut(s) 467, 1103, 1193
BslI CCNNNNNNNGG 4 cut(s) 48, 329, 1193, 1233
BsmAI GTCTC 2 cut(s) 674, 1197
BsnI GGCC 1 cut(s) 872
Bsp119I TTCGAA 1 cut(s) 1085
Bsp1286I GDGCHC 2 cut(s) 245, 1290
Bsp13I TCCGGA 2 cut(s) 466, 1102
Bsp1407I TGTACA 1 cut(s) 145
Bsp143I GATC 5 cut(s) 337, 643, 807, 1026, 1310
BspACI CCGC 3 cut(s) 109, 333, 508
BspANI GGCC 1 cut(s) 872
BspCNI CTCAG 2 cut(s) 906, 940
BspEI TCCGGA 2 cut(s) 466, 1102
BspLI GGNNCC 1 cut(s) 557
BspPI GGATC 1 cut(s) 1318
BspQI GCTCTTC 1 cut(s) 1295
BspT104I TTCGAA 1 cut(s) 1085
BspTI CTTAAG 1 cut(s) 830
BsrBI CCGCTC 1 cut(s) 510
BsrDI GCAATG 2 cut(s) 646, 1179
BsrGI TGTACA 1 cut(s) 145
BssECI CCNNGG 1 cut(s) 404
BssMI GATC 5 cut(s) 337, 643, 807, 1026, 1310
BssSI CACGAG 2 cut(s) 664, 1077
BssT1I CCWWGG 1 cut(s) 404
Bst2BI CACGAG 2 cut(s) 664, 1077
Bst4CI ACNGT 2 cut(s) 914, 1143
Bst6I CTCTTC 2 cut(s) 601, 1295
BstAFI CTTAAG 1 cut(s) 830
BstAPI GCANNNNNTGC 1 cut(s) 1247
BstAUI TGTACA 1 cut(s) 145
BstBAI YACGTR 1 cut(s) 246
BstBI TTCGAA 1 cut(s) 1085
BstC8I GCNNGC 1 cut(s) 31
BstDEI CTNAG 5 cut(s) 350, 584, 704, 893, 927
BstF5I GGATG 3 cut(s) 100, 784, 790
BstHHI GCGC 1 cut(s) 349
BstKTI GATC 5 cut(s) 340, 646, 810, 1029, 1313
BstMAI GTCTC 2 cut(s) 674, 1197
BstMBI GATC 5 cut(s) 337, 643, 807, 1026, 1310
BstMWI GCNNNNNNNGC 5 cut(s) 26, 355, 869, 1247, 1285
BstNSI RCATGY 2 cut(s) 627, 948
BstSCI CCNGG 1 cut(s) 1192
BstSFI CTRYAG 1 cut(s) 288
BstV1I GCAGC 2 cut(s) 1037, 1040
BstV2I GAAGAC 1 cut(s) 1124
BsuRI GGCC 1 cut(s) 872
BtgZI GCGATG 2 cut(s) 98, 865
BtsCI GGATG 3 cut(s) 100, 784, 790
BtsIMutI CAGTG 2 cut(s) 653, 1022
Cac8I GCNNGC 1 cut(s) 31
CfoI GCGC 1 cut(s) 349
Cfr13I GGNCC 5 cut(s) 106, 469, 478, 487, 500
Csp6I GTAC 3 cut(s) 146, 607, 1074
CviAII CATG 6 cut(s) 234, 535, 560, 624, 698, 945
CviQI GTAC 3 cut(s) 146, 607, 1074
DdeI CTNAG 5 cut(s) 350, 584, 704, 893, 927
DpnI GATC 5 cut(s) 339, 645, 809, 1028, 1312
DpnII GATC 5 cut(s) 337, 643, 807, 1026, 1310
Eam1104I CTCTTC 2 cut(s) 601, 1295
EarI CTCTTC 2 cut(s) 601, 1295
Ecl136II GAGCTC 1 cut(s) 1288
Eco130I CCWWGG 1 cut(s) 404
Eco24I GRGCYC 1 cut(s) 1290
Eco32I GATATC 2 cut(s) 316, 591
Eco47I GGWCC 5 cut(s) 106, 469, 478, 487, 500
Eco53kI GAGCTC 1 cut(s) 1288
EcoICRI GAGCTC 1 cut(s) 1288
EcoRV GATATC 2 cut(s) 316, 591
EcoT14I CCWWGG 1 cut(s) 404
EcoT22I ATGCAT 1 cut(s) 959
EcoT38I GRGCYC 1 cut(s) 1290
ErhI CCWWGG 1 cut(s) 404
FaeI CATG 6 cut(s) 237, 538, 563, 627, 701, 948
FatI CATG 6 cut(s) 233, 534, 559, 623, 697, 944
FauNDI CATATG 1 cut(s) 815
FbaI TGATCA 1 cut(s) 643
Fnu4HI GCNGC 3 cut(s) 334, 1051, 1054
FokI GGATG 3 cut(s) 107, 791, 797
FriOI GRGCYC 1 cut(s) 1290
Fsp4HI GCNGC 3 cut(s) 334, 1051, 1054
FspBI CTAG 3 cut(s) 185, 438, 494
GlaI GCGC 1 cut(s) 348
GluI GCNGC 3 cut(s) 334, 1051, 1054
GsaI CCCAGC 1 cut(s) 367
GsuI CTGGAG 1 cut(s) 470
HaeIII GGCC 1 cut(s) 872
HapII CCGG 3 cut(s) 467, 1103, 1193
HhaI GCGC 1 cut(s) 349
Hin1II CATG 6 cut(s) 237, 538, 563, 627, 701, 948
Hin6I GCGC 1 cut(s) 347
HinP1I GCGC 1 cut(s) 347
HincII GTYRAC 1 cut(s) 411
HindII GTYRAC 1 cut(s) 411
HinfI GANTC 7 cut(s) 667, 730, 737, 1082, 1106, 1180, 1223
HpaII CCGG 3 cut(s) 467, 1103, 1193
HphI GGTGA 1 cut(s) 250
Hpy166II GTNNAC 3 cut(s) 411, 658, 1020
Hpy188I TCNGA 5 cut(s) 722, 896, 1164, 1188, 1315
Hpy8I GTNNAC 3 cut(s) 411, 658, 1020
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 6 cut(s) 47, 79, 482, 491, 500, 1054
HpyCH4III ACNGT 2 cut(s) 914, 1143
HpyCH4IV ACGT 3 cut(s) 158, 245, 654
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 355, 869, 1247, 1285
HpyF3I CTNAG 5 cut(s) 350, 584, 704, 893, 927
HpySE526I ACGT 3 cut(s) 158, 245, 654
Hsp92II CATG 6 cut(s) 237, 538, 563, 627, 701, 948
HspAI GCGC 1 cut(s) 347
Kpn2I TCCGGA 2 cut(s) 466, 1102
Ksp22I TGATCA 1 cut(s) 643
Kzo9I GATC 5 cut(s) 337, 643, 807, 1026, 1310
LguI GCTCTTC 1 cut(s) 1295
LmnI GCTCC 3 cut(s) 522, 561, 722
Lsp1109I GCAGC 2 cut(s) 1037, 1040
LweI GCATC 2 cut(s) 778, 842
MaeI CTAG 3 cut(s) 185, 438, 494
MaeII ACGT 3 cut(s) 158, 245, 654
MaeIII GTNAC 3 cut(s) 650, 967, 981
MalI GATC 5 cut(s) 339, 645, 809, 1028, 1312
MbiI CCGCTC 1 cut(s) 510
MboI GATC 5 cut(s) 337, 643, 807, 1026, 1310
MboII GAAGA 4 cut(s) 565, 588, 1129, 1282
MfeI CAATTG 1 cut(s) 1231
MhlI GDGCHC 2 cut(s) 245, 1290
MluCI AATT 9 cut(s) 82, 218, 711, 763, 836, 905, 960, 1012, 1231
MlyI GAGTC 2 cut(s) 676, 1217
MmeI TCCRAC 4 cut(s) 291, 700, 733, 929
Mph1103I ATGCAT 1 cut(s) 959
MroI TCCGGA 2 cut(s) 466, 1102
MseI TTAA 8 cut(s) 420, 434, 617, 687, 831, 939, 1038, 1260
MslI CAYNNNNRTG 1 cut(s) 548
MspA1I CMGCKG 1 cut(s) 548
MspCI CTTAAG 1 cut(s) 830
MspI CCGG 3 cut(s) 467, 1103, 1193
MspR9I CCNGG 1 cut(s) 1194
MunI CAATTG 1 cut(s) 1231
MwoI GCNNNNNNNGC 5 cut(s) 26, 355, 869, 1247, 1285
NciI CCSGG 1 cut(s) 1194
NdeI CATATG 1 cut(s) 815
NdeII GATC 5 cut(s) 337, 643, 807, 1026, 1310
NlaIII CATG 6 cut(s) 237, 538, 563, 627, 701, 948
NlaIV GGNNCC 1 cut(s) 557
NmuCI GTSAC 1 cut(s) 650
NsiI ATGCAT 1 cut(s) 959
NspI RCATGY 2 cut(s) 627, 948
NspV TTCGAA 1 cut(s) 1085
OliI CACNNNNGTG 1 cut(s) 548
PciSI GCTCTTC 1 cut(s) 1295
PfeI GAWTC 5 cut(s) 730, 737, 1082, 1106, 1180
PflMI CCANNNNNTGG 2 cut(s) 48, 1233
PkrI GCNGC 3 cut(s) 335, 1052, 1055
PleI GAGTC 2 cut(s) 675, 1217
PpsI GAGTC 2 cut(s) 675, 1217
Ppu21I YACGTR 1 cut(s) 246
Psp124BI GAGCTC 1 cut(s) 1290
Psp1406I AACGTT 1 cut(s) 158
PspFI CCCAGC 1 cut(s) 363
PspN4I GGNNCC 1 cut(s) 557
PspPI GGNCC 5 cut(s) 106, 469, 478, 487, 500
PsrI GAACNNNNNNTAC 2 cut(s) 966, 998
PvuII CAGCTG 1 cut(s) 548
RsaI GTAC 3 cut(s) 147, 608, 1075
RsaNI GTAC 3 cut(s) 146, 607, 1074
RseI CAYNNNNRTG 1 cut(s) 548
SacI GAGCTC 1 cut(s) 1290
SapI GCTCTTC 1 cut(s) 1295
SaqAI TTAA 8 cut(s) 420, 434, 617, 687, 831, 939, 1038, 1260
SatI GCNGC 3 cut(s) 334, 1051, 1054
Sau3AI GATC 5 cut(s) 337, 643, 807, 1026, 1310
Sau96I GGNCC 5 cut(s) 106, 469, 478, 487, 500
ScaI AGTACT 1 cut(s) 608
SchI GAGTC 2 cut(s) 676, 1217
ScrFI CCNGG 1 cut(s) 1194
SduI GDGCHC 2 cut(s) 245, 1290
SfaNI GCATC 2 cut(s) 778, 842
SfcI CTRYAG 1 cut(s) 288
SfuI TTCGAA 1 cut(s) 1085
SinI GGWCC 5 cut(s) 106, 469, 478, 487, 500
SmiMI CAYNNNNRTG 1 cut(s) 548
SmlI CTYRAG 2 cut(s) 671, 830
SmoI CTYRAG 2 cut(s) 671, 830
SpeI ACTAGT 1 cut(s) 437
Sse9I AATT 9 cut(s) 82, 218, 711, 763, 836, 905, 960, 1012, 1231
SsiI CCGC 3 cut(s) 109, 333, 508
SspMI CTAG 3 cut(s) 185, 438, 494
SstI GAGCTC 1 cut(s) 1290
StyD4I CCNGG 1 cut(s) 1192
StyI CCWWGG 1 cut(s) 404
TaaI ACNGT 2 cut(s) 914, 1143
TaiI ACGT 3 cut(s) 161, 248, 657
TasI AATT 9 cut(s) 82, 218, 711, 763, 836, 905, 960, 1012, 1231
TatI WGTACW 3 cut(s) 145, 606, 1073
TauI GCSGC 1 cut(s) 336
TfiI GAWTC 5 cut(s) 730, 737, 1082, 1106, 1180
Tru1I TTAA 8 cut(s) 420, 434, 617, 687, 831, 939, 1038, 1260
Tru9I TTAA 8 cut(s) 420, 434, 617, 687, 831, 939, 1038, 1260
TscAI CASTG 2 cut(s) 653, 1029
TseFI GTSAC 1 cut(s) 650
TseI GCWGC 2 cut(s) 1050, 1053
Tsp45I GTSAC 1 cut(s) 650
TspDTI ATGAA 5 cut(s) 151, 218, 743, 759, 906
TspGWI ACGGA 1 cut(s) 1267
TspRI CASTG 2 cut(s) 653, 1029
Van91I CCANNNNNTGG 2 cut(s) 48, 1233
Vha464I CTTAAG 1 cut(s) 830
VpaK11BI GGWCC 5 cut(s) 106, 469, 478, 487, 500
XapI RAATTY 3 cut(s) 82, 905, 1012
XbaI TCTAGA 1 cut(s) 184
XceI RCATGY 2 cut(s) 627, 948
XspI CTAG 3 cut(s) 185, 438, 494
ZrmI AGTACT 1 cut(s) 608
Zsp2I ATGCAT 1 cut(s) 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.