Rmu_ssc0000204.1_g000057

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000204.1
OG0004540
Physical Location & Seq
Forward (+)
254443 .. 256971
2529 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000204.1_g000057.1.cds

Sequence Viewer

Length: 774 bp
atgccatcgtgcactgaggccacaattaacggatatgtcaatttgttcgagttctttgattgttatctgaatgccaatttttttgttaaagccaatgaggctgcaattctagtcggggaacttttggaccaagaatggttctcgtcatattcgacacattttcctaggctcatgagctttcttagaagatttagacgctgcccgaaaataaatctctacaatcaagatgttcaggctctcatcgttcccaagaattatagaaaagtgacctcctttccgccattgcctaatactttcactttgcaggcgtggatgccaaatccaccaatagtgggaagtatagattacttggaattgtgggactccttgcaatggattgcaccttttgcacagatattgataatgccggtggacgttgaagaagacagaaagaaagtagaccagcgatctagtccgtccgacattaacctccaccttccggtgactagactcaccggaggaaccttggtcatctccatcaagttcgttccaacagccacttcgagctccacctctgcaaaggccggtgtacactggttccggcgattgaagcagtctcaggctttggccgttccagtgaccaaaggtccaagctcaagcactgctacagtggaggccgacatggttcccgccaccagagccgtcgctgacatagagaacggcatcagcctccaccttcctgtcaccggagcaatctctgacaccagagctctccactcgaaacaagtctcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

257

Amino Acids

28.66

Weight (kDa)

8.42

Isoelectric Point (pI)

54.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 438
AciI CCGC 2 cut(s) 278, 669
AcoI YGGCCR 1 cut(s) 606
AfaI GTAC 1 cut(s) 570
AfiI CCNNNNNNNGG 4 cut(s) 332, 372, 478, 725
AgsI TTSAA 2 cut(s) 419, 589
AhdI GACNNNNNGTC 1 cut(s) 624
AjuI GAANNNNNNNTTGG 2 cut(s) 523, 555
AluBI AGCT 4 cut(s) 177, 546, 633, 749
AluI AGCT 4 cut(s) 177, 546, 633, 749
Alw21I GWGCWC 3 cut(s) 14, 548, 751
Alw26I GTCTC 1 cut(s) 600
Alw44I GTGCAC 1 cut(s) 10
AoxI GGCC 4 cut(s) 18, 561, 606, 654
ApaLI GTGCAC 1 cut(s) 10
ApeKI GCWGC 2 cut(s) 101, 198
AspA2I CCTAGG 1 cut(s) 164
AspS9I GGNCC 2 cut(s) 127, 626
AsuHPI GGTGA 3 cut(s) 484, 493, 715
AvaII GGWCC 2 cut(s) 127, 626
AvrII CCTAGG 1 cut(s) 164
BaeGI GKGCMC 1 cut(s) 14
BanII GRGCYC 2 cut(s) 548, 751
BbsI GAAGAC 1 cut(s) 429
Bbv12I GWGCWC 3 cut(s) 14, 548, 751
BbvI GCAGC 2 cut(s) 88, 185
BccI CCATC 2 cut(s) 13, 524
BceAI ACGGC 3 cut(s) 593, 665, 715
BcoDI GTCTC 1 cut(s) 600
BfaI CTAG 4 cut(s) 110, 165, 450, 486
BfmI CTRYAG 1 cut(s) 645
BglI GCCNNNNNGGC 1 cut(s) 98
BisI GCNGC 2 cut(s) 102, 199
BlnI CCTAGG 1 cut(s) 164
BlsI GCNGC 2 cut(s) 103, 200
Bme18I GGWCC 2 cut(s) 127, 626
BmeRI GACNNNNNGTC 1 cut(s) 624
BmgT120I GGNCC 2 cut(s) 127, 626
BmiI GGNNCC 3 cut(s) 502, 578, 666
BmsI GCATC 2 cut(s) 303, 711
BpiI GAAGAC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 619
BsaBI GATNNNNATC 1 cut(s) 63
BsaJI CCNNGG 2 cut(s) 164, 504
BsaWI WCCGGW 3 cut(s) 478, 494, 725
Bsc4I CCNNNNNNNGG 4 cut(s) 332, 372, 478, 725
Bse118I RCCGGY 2 cut(s) 406, 563
Bse1I ACTGG 2 cut(s) 578, 614
Bse3DI GCAATG 2 cut(s) 281, 377
Bse8I GATNNNNATC 1 cut(s) 63
BseDI CCNNGG 2 cut(s) 164, 504
BseGI GGATG 1 cut(s) 318
BseJI GATNNNNATC 1 cut(s) 63
BseLI CCNNNNNNNGG 4 cut(s) 332, 372, 478, 725
BseMI GCAATG 2 cut(s) 281, 377
BseMII CTCAG 2 cut(s) 6, 611
BseNI ACTGG 2 cut(s) 578, 614
BseSI GKGCMC 1 cut(s) 14
BseXI GCAGC 2 cut(s) 88, 185
BshFI GGCC 4 cut(s) 20, 563, 608, 656
BsiHKAI GWGCWC 3 cut(s) 14, 548, 751
BsiSI CCGG 6 cut(s) 407, 479, 495, 564, 580, 726
BslFI GGGAC 1 cut(s) 374
BslI CCNNNNNNNGG 4 cut(s) 332, 372, 478, 725
BsmAI GTCTC 1 cut(s) 600
BsmFI GGGAC 1 cut(s) 374
BsmI GAATGC 1 cut(s) 76
BsnI GGCC 4 cut(s) 20, 563, 608, 656
Bsp1286I GDGCHC 3 cut(s) 14, 548, 751
Bsp1407I TGTACA 1 cut(s) 568
Bsp143I GATC 1 cut(s) 446
BspACI CCGC 2 cut(s) 278, 669
BspANI GGCC 4 cut(s) 20, 563, 608, 656
BspCNI CTCAG 2 cut(s) 7, 610
BspHI TCATGA 2 cut(s) 171, 770
BspLI GGNNCC 3 cut(s) 502, 578, 666
BsrDI GCAATG 2 cut(s) 281, 377
BsrFI RCCGGY 2 cut(s) 406, 563
BsrGI TGTACA 1 cut(s) 568
BsrI ACTGG 2 cut(s) 578, 614
BssAI RCCGGY 2 cut(s) 406, 563
BssECI CCNNGG 2 cut(s) 164, 504
BssMI GATC 1 cut(s) 446
BssT1I CCWWGG 2 cut(s) 164, 504
Bst4CI ACNGT 1 cut(s) 649
BstAPI GCANNNNNTGC 1 cut(s) 386
BstAUI TGTACA 1 cut(s) 568
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 3 cut(s) 15, 182, 597
BstF5I GGATG 1 cut(s) 318
BstKTI GATC 1 cut(s) 449
BstMAI GTCTC 1 cut(s) 600
BstMBI GATC 1 cut(s) 446
BstMWI GCNNNNNNNGC 4 cut(s) 98, 386, 589, 677
BstSFI CTRYAG 1 cut(s) 645
BstSLI GKGCMC 1 cut(s) 14
BstV1I GCAGC 2 cut(s) 88, 185
BstV2I GAAGAC 1 cut(s) 429
BsuRI GGCC 4 cut(s) 20, 563, 608, 656
BtsCI GGATG 1 cut(s) 318
BtsI GCAGTG 1 cut(s) 639
BtsIMutI CAGTG 5 cut(s) 12, 571, 621, 639, 654
Cac8I GCNNGC 1 cut(s) 306
CciI TCATGA 2 cut(s) 171, 770
Cfr10I RCCGGY 2 cut(s) 406, 563
Cfr13I GGNCC 2 cut(s) 127, 626
CseI GACGC 1 cut(s) 204
Csp6I GTAC 1 cut(s) 569
CviAII CATG 3 cut(s) 172, 661, 771
CviQI GTAC 1 cut(s) 569
DdeI CTNAG 3 cut(s) 15, 182, 597
DpnI GATC 1 cut(s) 448
DpnII GATC 1 cut(s) 446
DriI GACNNNNNGTC 1 cut(s) 624
EaeI YGGCCR 1 cut(s) 606
Eam1105I GACNNNNNGTC 1 cut(s) 624
EciI GGCGGA 1 cut(s) 267
Ecl136II GAGCTC 2 cut(s) 546, 749
Eco130I CCWWGG 2 cut(s) 164, 504
Eco24I GRGCYC 2 cut(s) 548, 751
Eco47I GGWCC 2 cut(s) 127, 626
Eco53kI GAGCTC 2 cut(s) 546, 749
EcoICRI GAGCTC 2 cut(s) 546, 749
EcoT14I CCWWGG 2 cut(s) 164, 504
EcoT38I GRGCYC 2 cut(s) 548, 751
ErhI CCWWGG 2 cut(s) 164, 504
FaeI CATG 3 cut(s) 175, 664, 774
FaiI YATR 8 cut(s) 36, 148, 173, 258, 341, 662, 692, 772
FaqI GGGAC 1 cut(s) 374
FatI CATG 3 cut(s) 171, 660, 770
FauI CCCGC 1 cut(s) 676
FblI GTMKAC 1 cut(s) 438
Fnu4HI GCNGC 2 cut(s) 102, 199
FokI GGATG 1 cut(s) 325
FriOI GRGCYC 2 cut(s) 548, 751
Fsp4HI GCNGC 2 cut(s) 102, 199
FspBI CTAG 4 cut(s) 110, 165, 450, 486
GluI GCNGC 2 cut(s) 102, 199
HaeIII GGCC 4 cut(s) 20, 563, 608, 656
HapII CCGG 6 cut(s) 407, 479, 495, 564, 580, 726
HgaI GACGC 1 cut(s) 204
Hin1II CATG 3 cut(s) 175, 664, 774
HinfI GANTC 2 cut(s) 362, 489
HpaII CCGG 6 cut(s) 407, 479, 495, 564, 580, 726
HphI GGTGA 3 cut(s) 484, 493, 715
Hpy166II GTNNAC 5 cut(s) 12, 412, 439, 569, 571
Hpy188I TCNGA 3 cut(s) 69, 460, 739
Hpy188III TCNNGA 3 cut(s) 172, 224, 771
Hpy8I GTNNAC 5 cut(s) 12, 412, 439, 569, 571
Hpy99I CGWCG 1 cut(s) 686
HpyAV CCTTC 2 cut(s) 485, 725
HpyCH4III ACNGT 1 cut(s) 649
HpyCH4IV ACGT 1 cut(s) 414
HpyCH4V TGCA 7 cut(s) 12, 104, 304, 370, 380, 389, 557
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 386, 589, 677
HpyF3I CTNAG 3 cut(s) 15, 182, 597
HpySE526I ACGT 1 cut(s) 414
Hsp92II CATG 3 cut(s) 175, 664, 774
Kzo9I GATC 1 cut(s) 446
LmnI GCTCC 2 cut(s) 551, 728
Lsp1109I GCAGC 2 cut(s) 88, 185
LweI GCATC 2 cut(s) 303, 711
MaeI CTAG 4 cut(s) 110, 165, 450, 486
MaeII ACGT 1 cut(s) 414
MaeIII GTNAC 4 cut(s) 265, 481, 616, 721
MalI GATC 1 cut(s) 448
MboI GATC 1 cut(s) 446
MboII GAAGA 3 cut(s) 198, 431, 434
MhlI GDGCHC 3 cut(s) 14, 548, 751
MluCI AATT 6 cut(s) 24, 40, 76, 105, 253, 353
MlyI GAGTC 2 cut(s) 356, 483
MmeI TCCRAC 2 cut(s) 483, 554
MnlI CCTC 8 cut(s) 10, 91, 280, 479, 491, 562, 646, 719
MseI TTAA 3 cut(s) 27, 87, 465
MspI CCGG 6 cut(s) 407, 479, 495, 564, 580, 726
Mva1269I GAATGC 1 cut(s) 76
MwoI GCNNNNNNNGC 4 cut(s) 98, 386, 589, 677
NdeII GATC 1 cut(s) 446
NlaIII CATG 3 cut(s) 175, 664, 774
NlaIV GGNNCC 3 cut(s) 502, 578, 666
NmuCI GTSAC 4 cut(s) 265, 481, 616, 721
PagI TCATGA 2 cut(s) 171, 770
PcsI WCGNNNNNNNCGW 1 cut(s) 149
PctI GAATGC 1 cut(s) 76
PkrI GCNGC 2 cut(s) 103, 200
PleI GAGTC 2 cut(s) 356, 483
PpsI GAGTC 2 cut(s) 356, 483
Psp124BI GAGCTC 2 cut(s) 548, 751
PspN4I GGNNCC 3 cut(s) 502, 578, 666
PspPI GGNCC 2 cut(s) 127, 626
RsaI GTAC 1 cut(s) 570
RsaNI GTAC 1 cut(s) 569
SacI GAGCTC 2 cut(s) 548, 751
SaqAI TTAA 3 cut(s) 27, 87, 465
SatI GCNGC 2 cut(s) 102, 199
Sau3AI GATC 1 cut(s) 446
Sau96I GGNCC 2 cut(s) 127, 626
SchI GAGTC 2 cut(s) 356, 483
SduI GDGCHC 3 cut(s) 14, 548, 751
SfaNI GCATC 2 cut(s) 303, 711
SfcI CTRYAG 1 cut(s) 645
SinI GGWCC 2 cut(s) 127, 626
SmlI CTYRAG 1 cut(s) 634
SmoI CTYRAG 1 cut(s) 634
Sse9I AATT 6 cut(s) 24, 40, 76, 105, 253, 353
SsiI CCGC 2 cut(s) 278, 669
SspMI CTAG 4 cut(s) 110, 165, 450, 486
SstI GAGCTC 2 cut(s) 548, 751
StyI CCWWGG 2 cut(s) 164, 504
TaaI ACNGT 1 cut(s) 649
TaiI ACGT 1 cut(s) 417
TaqI TCGA 4 cut(s) 48, 152, 542, 758
TasI AATT 6 cut(s) 24, 40, 76, 105, 253, 353
TatI WGTACW 1 cut(s) 568
Tru1I TTAA 3 cut(s) 27, 87, 465
Tru9I TTAA 3 cut(s) 27, 87, 465
TscAI CASTG 5 cut(s) 19, 578, 621, 646, 654
TseFI GTSAC 4 cut(s) 265, 481, 616, 721
TseI GCWGC 2 cut(s) 101, 198
Tsp45I GTSAC 4 cut(s) 265, 481, 616, 721
TspGWI ACGGA 2 cut(s) 45, 444
TspRI CASTG 5 cut(s) 19, 578, 621, 646, 654
VneI GTGCAC 1 cut(s) 10
VpaK11BI GGWCC 2 cut(s) 127, 626
XmaJI CCTAGG 1 cut(s) 164
XmiI GTMKAC 1 cut(s) 438
XspI CTAG 4 cut(s) 110, 165, 450, 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.