Rw4G033970

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004540
Physical Location & Seq
Reverse (-)
61109533 .. 61110903
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033970.1

Sequence Viewer

Length: 1371 bp
ATGTCTGACATTCCCGATCTTGTTATTGACAAAATCCTTGGACTCATTCCTACTAAAGCTGCGTTCCAGGTGAGCTTTCTTTGCAAGCAACGGCAAGATGTAGTCTCCTCATTCCCTGTACTCAATTTCAACGAGGATGATGATTTTGACCGCCACAACCTTAATCGTTACCGTCACTACAAACATCAACACAAAAGGTTCATCAATTTCTTGAAGGGCTATTTAGATTATTGTAAAAAGAAGGACCAGAAAGAGCTCCTGAATAAATTAAGTCTTCACATGAGGATGTTTTCGCGTCGAGATTCATCTACCATAACCGAGTGGTTGAAATATGCATGTGAGAGAGGCCTCAAAGAGTTGGATATCAGTCCTAAAATGAGTGTTGACCCAACGAGTTGGATTGTCAGTGAGCGCCCACCGGAAAGTCTCTACCTCTTCTACATCTTGCCTTGGTTGGCGATTGCTAGTGCAAAATCATCTTTAACTAGTCTAAAGTTGGAGTGTGTGAGAGTTCCGCATCTGCTGCATGTAAGAGTTCCGCACGCTTATGAGCGACTTTTTCCATCCTTGAAAACTCTGTCCTTCAAAACTTCACGGGTTGTTGATCTCGATTCTCTTCTATTTGAGTGCCCGTCCATGGAGTACCTGTCATTAACTCAATGTTCGATTGGAAACTCCGAGTTTTATGTTCAATGCTCAAGTCTTAAGTCCTTGGAAATTAAGTATTGCCGTCTTCATGTTATTCGAGTTGATCAAGCCTTCAATCTTGAATCTTTTACATTTCTCTCACCGCCGACATATTCAAGATGTGAAAAGATGATCTTGAAGAATGCCTTCAACTTGAAACATATCAACATTTGTGTTGATTACCTCCGGGAATTTTCTTTATTGGGAAGCCATCGTGCACTAGAGGCCACAATTGACCGATATGTCCATTTGTTCGAGTTCTTTGATGGTTATTTGAATGCCAACGTTTCTGTTAAAGCCTATGAGGCCACAATTTTAGTTGGGGAACTTTTGGACCAAGAATGGTTCTCGTCATATTCGACACATTTTCCGAAGCTGATGACCTTTCTTAGAAAATTTAGATGCTGCTCGAAAATAAACCTCTACGTTCAGGCTCTCATCATTCCAAAGAATTATAGAAAGATGACCTCCGTTCCACCATTGCCGGAAACTTTCACTTTGCAGGCATGGATGTCGAATCCACCACTAGTGGGAAGTAAAGTCTATTTGGAAATGGAGGACTCCTTGCAATGGATGGCACCACTGGCACAGATATTGATCATGCCGGTGGACGTTGAAGAAGATAGAAAGAAAGTATACGAGCGAGCTAGTCCTGTAGGACATGGACGAATGAGAATGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

456

Amino Acids

53.29

Weight (kDa)

8.82

Isoelectric Point (pI)

45.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 154 - 284 1.6e-08 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 929
AccB1I GGYRCC 1 cut(s) 1264
AccI GTMKAC 1 cut(s) 1323
AccII CGCG 1 cut(s) 295
AciI CCGC 4 cut(s) 151, 515, 539, 791
AclI AACGTT 1 cut(s) 972
AcsI RAATTY 2 cut(s) 878, 1082
AfaI GTAC 2 cut(s) 120, 644
AfiI CCNNNNNNNGG 3 cut(s) 637, 1217, 1257
AflII CTTAAG 1 cut(s) 704
AhlI ACTAGT 2 cut(s) 485, 1213
AjnI CCWGG 1 cut(s) 66
AluBI AGCT 5 cut(s) 59, 75, 256, 1063, 1334
AluI AGCT 5 cut(s) 59, 75, 256, 1063, 1334
Alw21I GWGCWC 2 cut(s) 258, 907
Alw26I GTCTC 2 cut(s) 109, 431
Alw44I GTGCAC 1 cut(s) 903
AoxI GGCC 3 cut(s) 346, 912, 993
ApaLI GTGCAC 1 cut(s) 903
ApeKI GCWGC 3 cut(s) 59, 523, 1092
ApoI RAATTY 2 cut(s) 878, 1082
Asp700I GAANNNNTTC 1 cut(s) 833
AspLEI GCGC 1 cut(s) 414
AspS9I GGNCC 2 cut(s) 244, 1021
AsuC2I CCSGG 1 cut(s) 875
AsuHPI GGTGA 2 cut(s) 82, 780
AvaII GGWCC 2 cut(s) 244, 1021
BaeGI GKGCMC 2 cut(s) 632, 907
BanI GGYRCC 1 cut(s) 1264
BanII GRGCYC 1 cut(s) 258
BbsI GAAGAC 2 cut(s) 266, 725
Bbv12I GWGCWC 2 cut(s) 258, 907
BbvI GCAGC 3 cut(s) 46, 510, 1079
BccI CCATC 4 cut(s) 571, 906, 947, 1255
BceAI ACGGC 2 cut(s) 107, 714
BcgI CGANNNNNNTGC 2 cut(s) 1182, 1216
BciT130I CCWGG 1 cut(s) 68
BclI TGATCA 2 cut(s) 751, 1284
BcnI CCSGG 1 cut(s) 875
BcoDI GTCTC 2 cut(s) 109, 431
BcuI ACTAGT 2 cut(s) 485, 1213
BfaI CTAG 5 cut(s) 465, 486, 908, 1214, 1335
BfmI CTRYAG 1 cut(s) 1341
BfoI RGCGCY 1 cut(s) 415
BfrI CTTAAG 1 cut(s) 704
BglI GCCNNNNNGGC 1 cut(s) 992
BisI GCNGC 3 cut(s) 60, 524, 1093
BlsI GCNGC 3 cut(s) 61, 525, 1094
Bme1390I CCNGG 2 cut(s) 68, 875
Bme18I GGWCC 2 cut(s) 244, 1021
BmgT120I GGNCC 2 cut(s) 244, 1021
BmiI GGNNCC 1 cut(s) 1266
BmrFI CCNGG 2 cut(s) 68, 875
BmsI GCATC 2 cut(s) 526, 1079
BpiI GAAGAC 2 cut(s) 266, 725
BpuEI CTTGAG 1 cut(s) 682
BpuMI CCSGG 1 cut(s) 875
BsaBI GATNNNNATC 1 cut(s) 1283
BsaJI CCNNGG 4 cut(s) 37, 449, 636, 711
BsaWI WCCGGW 1 cut(s) 418
BsaXI ACNNNNNCTCC 2 cut(s) 632, 662
Bsc4I CCNNNNNNNGG 3 cut(s) 637, 1217, 1257
Bse118I RCCGGY 1 cut(s) 1291
Bse1I ACTGG 1 cut(s) 1275
Bse3DI GCAATG 2 cut(s) 1166, 1262
Bse8I GATNNNNATC 1 cut(s) 1283
BseBI CCWGG 1 cut(s) 68
BseDI CCNNGG 4 cut(s) 37, 449, 636, 711
BseGI GGATG 5 cut(s) 142, 291, 563, 1203, 1266
BseJI GATNNNNATC 1 cut(s) 1283
BseLI CCNNNNNNNGG 3 cut(s) 637, 1217, 1257
BseMI GCAATG 2 cut(s) 1166, 1262
BseNI ACTGG 1 cut(s) 1275
BseRI GAGGAG 1 cut(s) 97
BseSI GKGCMC 2 cut(s) 632, 907
BseXI GCAGC 3 cut(s) 46, 510, 1079
Bsh1236I CGCG 1 cut(s) 295
BshFI GGCC 3 cut(s) 348, 914, 995
BshNI GGYRCC 1 cut(s) 1264
BsiHKAI GWGCWC 2 cut(s) 258, 907
BsiSI CCGG 4 cut(s) 419, 874, 1172, 1292
BslI CCNNNNNNNGG 3 cut(s) 637, 1217, 1257
BsmAI GTCTC 2 cut(s) 109, 431
BsmI GAATGC 2 cut(s) 835, 970
BsnI GGCC 3 cut(s) 348, 914, 995
Bsp1286I GDGCHC 3 cut(s) 258, 632, 907
Bsp143I GATC 5 cut(s) 16, 604, 751, 819, 1284
Bsp19I CCATGG 1 cut(s) 636
BspACI CCGC 4 cut(s) 151, 515, 539, 791
BspANI GGCC 3 cut(s) 348, 914, 995
BspFNI CGCG 1 cut(s) 295
BspLI GGNNCC 1 cut(s) 1266
BspT107I GGYRCC 1 cut(s) 1264
BspTI CTTAAG 1 cut(s) 704
BsrDI GCAATG 2 cut(s) 1166, 1262
BsrFI RCCGGY 1 cut(s) 1291
BsrI ACTGG 1 cut(s) 1275
BssAI RCCGGY 1 cut(s) 1291
BssECI CCNNGG 4 cut(s) 37, 449, 636, 711
BssMI GATC 5 cut(s) 16, 604, 751, 819, 1284
BssNAI GTATAC 1 cut(s) 1324
BssT1I CCWWGG 4 cut(s) 37, 449, 636, 711
Bst1107I GTATAC 1 cut(s) 1324
Bst2UI CCWGG 1 cut(s) 68
Bst4CI ACNGT 1 cut(s) 173
Bst6I CTCTTC 2 cut(s) 440, 621
BstAFI CTTAAG 1 cut(s) 704
BstAPI GCANNNNNTGC 1 cut(s) 523
BstC8I GCNNGC 4 cut(s) 86, 543, 1191, 1332
BstDEI CTNAG 1 cut(s) 1076
BstDSI CCRYGG 1 cut(s) 636
BstF5I GGATG 5 cut(s) 142, 291, 563, 1203, 1266
BstFNI CGCG 1 cut(s) 295
BstH2I RGCGCY 1 cut(s) 415
BstHHI GCGC 1 cut(s) 414
BstKTI GATC 5 cut(s) 19, 607, 754, 822, 1287
BstMAI GTCTC 2 cut(s) 109, 431
BstMBI GATC 5 cut(s) 16, 604, 751, 819, 1284
BstMWI GCNNNNNNNGC 5 cut(s) 81, 523, 911, 992, 1271
BstNI CCWGG 1 cut(s) 68
BstNSI RCATGY 2 cut(s) 339, 530
BstSCI CCNGG 2 cut(s) 66, 873
BstSFI CTRYAG 1 cut(s) 1341
BstSLI GKGCMC 2 cut(s) 632, 907
BstUI CGCG 1 cut(s) 295
BstV1I GCAGC 3 cut(s) 46, 510, 1079
BstV2I GAAGAC 2 cut(s) 266, 725
BstXI CCANNNNNNTGG 1 cut(s) 396
BstZ17I GTATAC 1 cut(s) 1324
BsuRI GGCC 3 cut(s) 348, 914, 995
BtgI CCRYGG 1 cut(s) 636
BtsCI GGATG 5 cut(s) 142, 291, 563, 1203, 1266
BtsIMutI CAGTG 2 cut(s) 412, 1268
Cac8I GCNNGC 4 cut(s) 86, 543, 1191, 1332
CfoI GCGC 1 cut(s) 414
Cfr10I RCCGGY 1 cut(s) 1291
Cfr13I GGNCC 2 cut(s) 244, 1021
CseI GACGC 1 cut(s) 284
Csp6I GTAC 2 cut(s) 119, 643
CviAII CATG 8 cut(s) 280, 336, 527, 637, 737, 1194, 1288, 1349
CviQI GTAC 2 cut(s) 119, 643
DdeI CTNAG 1 cut(s) 1076
DpnI GATC 5 cut(s) 18, 606, 753, 821, 1286
DpnII GATC 5 cut(s) 16, 604, 751, 819, 1284
DrdI GACNNNNNNGTC 1 cut(s) 929
DseDI GACNNNNNNGTC 1 cut(s) 929
Eam1104I CTCTTC 2 cut(s) 440, 621
EarI CTCTTC 2 cut(s) 440, 621
Ecl136II GAGCTC 1 cut(s) 256
Eco130I CCWWGG 4 cut(s) 37, 449, 636, 711
Eco147I AGGCCT 1 cut(s) 348
Eco24I GRGCYC 1 cut(s) 258
Eco32I GATATC 1 cut(s) 364
Eco47I GGWCC 2 cut(s) 244, 1021
Eco53kI GAGCTC 1 cut(s) 256
EcoICRI GAGCTC 1 cut(s) 256
EcoRII CCWGG 1 cut(s) 66
EcoRV GATATC 1 cut(s) 364
EcoT14I CCWWGG 4 cut(s) 37, 449, 636, 711
EcoT22I ATGCAT 1 cut(s) 337
EcoT38I GRGCYC 1 cut(s) 258
ErhI CCWWGG 4 cut(s) 37, 449, 636, 711
FaeI CATG 8 cut(s) 283, 339, 530, 640, 740, 1197, 1291, 1352
FalI AAGNNNNNCTT 4 cut(s) 806, 838, 818, 850
FatI CATG 8 cut(s) 279, 335, 526, 636, 736, 1193, 1287, 1348
FbaI TGATCA 2 cut(s) 751, 1284
FblI GTMKAC 1 cut(s) 1323
Fnu4HI GCNGC 3 cut(s) 60, 524, 1093
FokI GGATG 5 cut(s) 149, 298, 550, 1210, 1273
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 3 cut(s) 60, 524, 1093
FspBI CTAG 5 cut(s) 465, 486, 908, 1214, 1335
GlaI GCGC 1 cut(s) 413
GluI GCNGC 3 cut(s) 60, 524, 1093
HaeII RGCGCY 1 cut(s) 415
HaeIII GGCC 3 cut(s) 348, 914, 995
HapII CCGG 4 cut(s) 419, 874, 1172, 1292
HgaI GACGC 1 cut(s) 284
HhaI GCGC 1 cut(s) 414
Hin1II CATG 8 cut(s) 283, 339, 530, 640, 740, 1197, 1291, 1352
Hin6I GCGC 1 cut(s) 412
HinP1I GCGC 1 cut(s) 412
HincII GTYRAC 1 cut(s) 385
HindII GTYRAC 1 cut(s) 385
HinfI GANTC 6 cut(s) 42, 302, 611, 770, 1204, 1247
HpaII CCGG 4 cut(s) 419, 874, 1172, 1292
HphI GGTGA 2 cut(s) 82, 780
Hpy166II GTNNAC 4 cut(s) 385, 905, 1297, 1324
Hpy188I TCNGA 3 cut(s) 7, 679, 1059
Hpy188III TCNNGA 8 cut(s) 14, 211, 259, 299, 608, 767, 804, 823
Hpy8I GTNNAC 4 cut(s) 385, 905, 1297, 1324
Hpy99I CGWCG 1 cut(s) 300
HpyAV CCTTC 5 cut(s) 208, 235, 592, 769, 844
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4IV ACGT 3 cut(s) 972, 1113, 1299
HpyCH4V TGCA 7 cut(s) 84, 335, 470, 526, 905, 1189, 1255
HpyF10VI GCNNNNNNNGC 5 cut(s) 81, 523, 911, 992, 1271
HpyF3I CTNAG 1 cut(s) 1076
HpySE526I ACGT 3 cut(s) 972, 1113, 1299
Hsp92II CATG 8 cut(s) 283, 339, 530, 640, 740, 1197, 1291, 1352
HspAI GCGC 1 cut(s) 412
Ksp22I TGATCA 2 cut(s) 751, 1284
Kzo9I GATC 5 cut(s) 16, 604, 751, 819, 1284
LmnI GCTCC 1 cut(s) 261
Lsp1109I GCAGC 3 cut(s) 46, 510, 1079
LweI GCATC 2 cut(s) 526, 1079
MaeI CTAG 5 cut(s) 465, 486, 908, 1214, 1335
MaeII ACGT 3 cut(s) 972, 1113, 1299
MaeIII GTNAC 2 cut(s) 167, 173
MalI GATC 5 cut(s) 18, 606, 753, 821, 1286
MboI GATC 5 cut(s) 16, 604, 751, 819, 1284
MboII GAAGA 7 cut(s) 266, 427, 608, 725, 838, 1316, 1319
MfeI CAATTG 1 cut(s) 918
MhlI GDGCHC 3 cut(s) 258, 632, 907
MluCI AATT 9 cut(s) 124, 205, 266, 717, 878, 918, 999, 1082, 1138
MlyI GAGTC 2 cut(s) 36, 1241
MmeI TCCRAC 3 cut(s) 339, 377, 477
Mph1103I ATGCAT 1 cut(s) 337
MroXI GAANNNNTTC 1 cut(s) 833
MseI TTAA 7 cut(s) 162, 269, 482, 653, 705, 720, 981
MslI CAYNNNNRTG 3 cut(s) 284, 546, 1292
MspCI CTTAAG 1 cut(s) 704
MspI CCGG 4 cut(s) 419, 874, 1172, 1292
MspR9I CCNGG 2 cut(s) 68, 875
MunI CAATTG 1 cut(s) 918
Mva1269I GAATGC 2 cut(s) 835, 970
MvaI CCWGG 1 cut(s) 68
MvnI CGCG 1 cut(s) 295
MwoI GCNNNNNNNGC 5 cut(s) 81, 523, 911, 992, 1271
NciI CCSGG 1 cut(s) 875
NcoI CCATGG 1 cut(s) 636
NdeII GATC 5 cut(s) 16, 604, 751, 819, 1284
NlaIII CATG 8 cut(s) 283, 339, 530, 640, 740, 1197, 1291, 1352
NlaIV GGNNCC 1 cut(s) 1266
NmuCI GTSAC 1 cut(s) 173
NsiI ATGCAT 1 cut(s) 337
NspI RCATGY 2 cut(s) 339, 530
PceI AGGCCT 1 cut(s) 348
PcsI WCGNNNNNNNCGW 1 cut(s) 1043
PctI GAATGC 2 cut(s) 835, 970
PdmI GAANNNNTTC 1 cut(s) 833
PfeI GAWTC 4 cut(s) 302, 611, 770, 1204
PfoI TCCNGGA 1 cut(s) 873
PkrI GCNGC 3 cut(s) 61, 525, 1094
PleI GAGTC 2 cut(s) 36, 1241
PpsI GAGTC 2 cut(s) 36, 1241
Psp124BI GAGCTC 1 cut(s) 258
Psp1406I AACGTT 1 cut(s) 972
Psp6I CCWGG 1 cut(s) 66
PspGI CCWGG 1 cut(s) 66
PspN4I GGNNCC 1 cut(s) 1266
PspPI GGNCC 2 cut(s) 244, 1021
RsaI GTAC 2 cut(s) 120, 644
RsaNI GTAC 2 cut(s) 119, 643
RseI CAYNNNNRTG 3 cut(s) 284, 546, 1292
SacI GAGCTC 1 cut(s) 258
SaqAI TTAA 7 cut(s) 162, 269, 482, 653, 705, 720, 981
SatI GCNGC 3 cut(s) 60, 524, 1093
Sau3AI GATC 5 cut(s) 16, 604, 751, 819, 1284
Sau96I GGNCC 2 cut(s) 244, 1021
SchI GAGTC 2 cut(s) 36, 1241
ScrFI CCNGG 2 cut(s) 68, 875
SduI GDGCHC 3 cut(s) 258, 632, 907
SfaNI GCATC 2 cut(s) 526, 1079
SfcI CTRYAG 1 cut(s) 1341
SinI GGWCC 2 cut(s) 244, 1021
SmiMI CAYNNNNRTG 3 cut(s) 284, 546, 1292
SmlI CTYRAG 2 cut(s) 697, 704
SmoI CTYRAG 2 cut(s) 697, 704
SpeI ACTAGT 2 cut(s) 485, 1213
Sse9I AATT 9 cut(s) 124, 205, 266, 717, 878, 918, 999, 1082, 1138
SseBI AGGCCT 1 cut(s) 348
SsiI CCGC 4 cut(s) 151, 515, 539, 791
SspMI CTAG 5 cut(s) 465, 486, 908, 1214, 1335
SstI GAGCTC 1 cut(s) 258
StuI AGGCCT 1 cut(s) 348
StyD4I CCNGG 2 cut(s) 66, 873
StyI CCWWGG 4 cut(s) 37, 449, 636, 711
TaaI ACNGT 1 cut(s) 173
TaiI ACGT 3 cut(s) 975, 1116, 1302
TaqI TCGA 8 cut(s) 298, 609, 665, 745, 942, 1046, 1097, 1202
TaqII GACCGA 1 cut(s) 939
TasI AATT 9 cut(s) 124, 205, 266, 717, 878, 918, 999, 1082, 1138
TatI WGTACW 1 cut(s) 118
TfiI GAWTC 4 cut(s) 302, 611, 770, 1204
Tru1I TTAA 7 cut(s) 162, 269, 482, 653, 705, 720, 981
Tru9I TTAA 7 cut(s) 162, 269, 482, 653, 705, 720, 981
TscAI CASTG 2 cut(s) 412, 1275
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 3 cut(s) 59, 523, 1092
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 3 cut(s) 190, 294, 725
TspGWI ACGGA 1 cut(s) 1147
TspRI CASTG 2 cut(s) 412, 1275
Vha464I CTTAAG 1 cut(s) 704
VneI GTGCAC 1 cut(s) 903
VpaK11BI GGWCC 2 cut(s) 244, 1021
XapI RAATTY 2 cut(s) 878, 1082
XceI RCATGY 2 cut(s) 339, 530
XmiI GTMKAC 1 cut(s) 1323
XmnI GAANNNNTTC 1 cut(s) 833
XspI CTAG 5 cut(s) 465, 486, 908, 1214, 1335
Zsp2I ATGCAT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.