RchiOBHm_Chr4g0442821

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004540
Physical Location & Seq
Forward (+)
64255985 .. 64257496
1512 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41056

Sequence Viewer

Length: 1512 bp
ATGCATTTTTATTTCTGTCGAGATGCTACTGTCATTGATAGGTGGCTGGAACGTGCAATTAATAGAGGTCTCAAAGAATTAGATATTAGCCCACGACAAATGACTATTATCCATGGAGAAATAGTGGTGGAAGCCGAAAAACTGTATCGCTTACCTTGGGTATCCTTTGCTAATAATGCACAATCTCTTACTAGTCTAAATCTGGAGTATGTTAGGATGGCAGACATCCAAAGTGCAAAACTTCAGAGGGATGAGAAGTACCTTCTTCCCTCCTTAAAAACCATGTCCTTCGAATGTGTGTGGTTTGATGACAAAGCTTTCTTCTCCTTACTATTGGAGTGCCCTTCCATCAAGTATTTGTCACTGACCGGCTGCACATTTGGAGACTTCACGTGTCGTGTTTCAAGTTCAAGTCTCGAGTTCCTGAAACTGAAGTTTTGCTTTCAGCTTGAGGTGCTTCAAGTTCATGATGAGACTAACAATCTTGAATCTTTTACTTTTTTTTCGGAGTCAGCACCATATTCACTATGTGAAAATGTGATCATGAATAAATCCTTCAAGTTGAAAAATATCAACATTTTTACGGGAAGTTACAACCTCTACGACTCCTACCATTTATCTTGGGTGACATTATTTGCTAATGCAAAATTCCTAACTAGTCTAAATCTGGAGGACACGAGAATTGCAAACATTAGTGCAAACGTTCAAAGTGACGAGCGCCTTCTTCCCTCTTTGAAAACCTTGTCCTTCGCAATTTCATGGTTTGCTGACGAAGCTCTCCCCTCCTTATTATTGGAGTGCCCTTCCATCGAGTATTTGTCAATAACTGAATGTTGTTTTGAAAACTTCAAGTGTCAAGTTTCAAGTTTAAATCTCAAGTCCTTGGAAGTTATGGATTGCGAACGGCTACAAGTTCTTCAAGTTGATGAAGCTAAGAACCTTGAATCTTTTACTTTTGCTTCTCCGCTTCCGTCACATGAAAAGGTGATCTTGAATAATACCTTCAACTTAAAAAATATCAACATTGGTGTTGACGACCTAGGGCAGTTTTCTTTAAACGGATGTCATCGTGCACTAAAAGCCACAATCAATGTCCAAAATCTCCATCTTTTTGATTTTCTTGGAACTTTGAAGGCTGAATTTTCTGTTAAAGGATGTCAGGGGGCCAAGATTGCAGTTCGGGAACTTTTGTTCAAAAATTGGTTCGTGCATTACTCTTCACATTTTCCTACATTGATTGGTTGTCTCAAAGAATTTGGGTGCTGCAAGAAAATATCCCTCTATAATCAAGATCTGAAGGCTCTCATAATTCCTAATCATTTTAGGAAGGAATTCTCTTCACCGTTGCCTAAAATTTGCACTCTGGAGGTGATGATGCCAAGTCCACCAACAAAAGCGAGTAGAGATTATTTCGACTTGATGGAATCCTTGCATTGGATTGCACCTTCTGCAAAAGTGAAAATCTGCAAAAGTGAAACACACCGTTTTGATGCATTATTAGAAGAACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

503

Amino Acids

57.68

Weight (kDa)

6.05

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 9 - 189 3.3e-07 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 12 - 172 3e-09 FBD-associated F-box protein At5g56370, LRR repeats
LRR_At5g56370 PF24758 217 - 322 9.9e-07 FBD-associated F-box protein At5g56370, LRR repeats
LRR_At1g61320_AtMIF1 PF23622 241 - 346 2.2e-06 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 965
AclI AACGTT 1 cut(s) 702
AcsI RAATTY 5 cut(s) 647, 1139, 1253, 1331, 1353
AcuI CTGAAG 3 cut(s) 227, 452, 1316
AcvI CACGTG 1 cut(s) 393
AdeI CACNNNGTG 1 cut(s) 530
AfaI GTAC 1 cut(s) 260
AfiI CCNNNNNNNGG 1 cut(s) 1434
AflIII ACRYGT 1 cut(s) 392
AhlI ACTAGT 2 cut(s) 191, 656
AloI GAACNNNNNNTCC 2 cut(s) 1175, 1207
AluBI AGCT 4 cut(s) 317, 448, 776, 932
AluI AGCT 4 cut(s) 317, 448, 776, 932
Alw21I GWGCWC 1 cut(s) 1075
Alw26I GTCTC 5 cut(s) 74, 378, 419, 467, 1250
Alw44I GTGCAC 1 cut(s) 1071
Ama87I CYCGRG 1 cut(s) 416
AoxI GGCC 1 cut(s) 1164
ApaLI GTGCAC 1 cut(s) 1071
ApeKI GCWGC 2 cut(s) 372, 1263
ApoI RAATTY 5 cut(s) 647, 1139, 1253, 1331, 1353
AseI ATTAAT 1 cut(s) 60
Asp700I GAANNNNTTC 1 cut(s) 1331
AspA2I CCTAGG 1 cut(s) 1039
AspLEI GCGC 1 cut(s) 720
AspS9I GGNCC 1 cut(s) 1164
AsuHPI GGTGA 4 cut(s) 637, 997, 1332, 1381
AsuII TTCGAA 1 cut(s) 291
AvaI CYCGRG 1 cut(s) 416
AvrII CCTAGG 1 cut(s) 1039
BaeGI GKGCMC 3 cut(s) 344, 803, 1075
BauI CACGAG 1 cut(s) 676
BbrPI CACGTG 1 cut(s) 393
Bbv12I GWGCWC 1 cut(s) 1075
BbvI GCAGC 2 cut(s) 359, 1250
BccI CCATC 5 cut(s) 211, 356, 815, 1113, 1414
BceAI ACGGC 1 cut(s) 920
BciVI GTATCC 1 cut(s) 172
BclI TGATCA 1 cut(s) 540
BcoDI GTCTC 5 cut(s) 74, 378, 419, 467, 1250
BcuI ACTAGT 2 cut(s) 191, 656
BfaI CTAG 3 cut(s) 192, 657, 1040
BfoI RGCGCY 1 cut(s) 721
BfuI GTATCC 1 cut(s) 172
BglII AGATCT 1 cut(s) 1291
BisI GCNGC 2 cut(s) 373, 1264
BlnI CCTAGG 1 cut(s) 1039
BlsI GCNGC 2 cut(s) 374, 1265
BmeT110I CYCGRG 1 cut(s) 416
BmgT120I GGNCC 1 cut(s) 1164
BmiI GGNNCC 1 cut(s) 1165
BmsI GCATC 3 cut(s) 13, 1365, 1480
BpmI CTGGAG 3 cut(s) 224, 689, 1385
Bpu14I TTCGAA 1 cut(s) 291
BpuEI CTTGAG 2 cut(s) 470, 860
BsaAI YACGTR 1 cut(s) 393
BsaI GGTCTC 1 cut(s) 74
BsaJI CCNNGG 4 cut(s) 112, 155, 882, 1039
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 1 cut(s) 1434
Bse118I RCCGGY 1 cut(s) 368
BseDI CCNNGG 4 cut(s) 112, 155, 882, 1039
BseGI GGATG 5 cut(s) 222, 225, 256, 1067, 1160
BseLI CCNNNNNNNGG 1 cut(s) 1434
BseSI GKGCMC 3 cut(s) 344, 803, 1075
BseXI GCAGC 2 cut(s) 359, 1250
BsgI GTGCAG 1 cut(s) 358
BshFI GGCC 1 cut(s) 1166
BsiHKAI GWGCWC 1 cut(s) 1075
BsiHKCI CYCGRG 1 cut(s) 416
BsiSI CCGG 1 cut(s) 369
BslI CCNNNNNNNGG 1 cut(s) 1434
BsmAI GTCTC 5 cut(s) 74, 378, 419, 467, 1250
BsnI GGCC 1 cut(s) 1166
Bso31I GGTCTC 1 cut(s) 74
BsoBI CYCGRG 1 cut(s) 416
Bsp119I TTCGAA 1 cut(s) 291
Bsp1286I GDGCHC 3 cut(s) 344, 803, 1075
Bsp143I GATC 3 cut(s) 540, 987, 1291
Bsp19I CCATGG 1 cut(s) 112
BspACI CCGC 1 cut(s) 965
BspANI GGCC 1 cut(s) 1166
BspHI TCATGA 2 cut(s) 466, 543
BspLI GGNNCC 1 cut(s) 1165
BspT104I TTCGAA 1 cut(s) 291
BspTNI GGTCTC 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 368
BssAI RCCGGY 1 cut(s) 368
BssECI CCNNGG 4 cut(s) 112, 155, 882, 1039
BssMI GATC 3 cut(s) 540, 987, 1291
BssSI CACGAG 1 cut(s) 676
BssT1I CCWWGG 4 cut(s) 112, 155, 882, 1039
Bst2BI CACGAG 1 cut(s) 676
Bst4CI ACNGT 4 cut(s) 31, 144, 1344, 1484
Bst6I CTCTTC 2 cut(s) 1222, 1342
BstAPI GCANNNNNTGC 1 cut(s) 1448
BstBAI YACGTR 1 cut(s) 393
BstBI TTCGAA 1 cut(s) 291
BstDEI CTNAG 1 cut(s) 933
BstDSI CCRYGG 1 cut(s) 112
BstF5I GGATG 5 cut(s) 222, 225, 256, 1067, 1160
BstH2I RGCGCY 1 cut(s) 721
BstHHI GCGC 1 cut(s) 720
BstKTI GATC 3 cut(s) 543, 990, 1294
BstMAI GTCTC 5 cut(s) 74, 378, 419, 467, 1250
BstMBI GATC 3 cut(s) 540, 987, 1291
BstMWI GCNNNNNNNGC 6 cut(s) 176, 454, 773, 1079, 1172, 1448
BstSLI GKGCMC 3 cut(s) 344, 803, 1075
BstV1I GCAGC 2 cut(s) 359, 1250
BstX2I RGATCY 1 cut(s) 1291
BstYI RGATCY 1 cut(s) 1291
BsuI GTATCC 1 cut(s) 172
BsuRI GGCC 1 cut(s) 1166
BtgI CCRYGG 1 cut(s) 112
BtsCI GGATG 5 cut(s) 222, 225, 256, 1067, 1160
BtsIMutI CAGTG 1 cut(s) 362
CciI TCATGA 2 cut(s) 466, 543
CfoI GCGC 1 cut(s) 720
Cfr10I RCCGGY 1 cut(s) 368
Cfr13I GGNCC 1 cut(s) 1164
Csp6I GTAC 1 cut(s) 259
CviAII CATG 6 cut(s) 113, 283, 467, 544, 759, 977
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 1 cut(s) 933
DpnI GATC 3 cut(s) 542, 989, 1293
DpnII GATC 3 cut(s) 540, 987, 1291
DraI TTTAAA 2 cut(s) 870, 1056
DraIII CACNNNGTG 1 cut(s) 530
Eam1104I CTCTTC 2 cut(s) 1222, 1342
EarI CTCTTC 2 cut(s) 1222, 1342
Eco130I CCWWGG 4 cut(s) 112, 155, 882, 1039
Eco31I GGTCTC 1 cut(s) 74
Eco57I CTGAAG 3 cut(s) 227, 452, 1316
Eco72I CACGTG 1 cut(s) 393
Eco88I CYCGRG 1 cut(s) 416
EcoRI GAATTC 1 cut(s) 1331
EcoT14I CCWWGG 4 cut(s) 112, 155, 882, 1039
EcoT22I ATGCAT 2 cut(s) 6, 1495
ErhI CCWWGG 4 cut(s) 112, 155, 882, 1039
FaeI CATG 6 cut(s) 116, 286, 470, 547, 762, 980
FalI AAGNNNNNCTT 4 cut(s) 425, 457, 974, 1006
FatI CATG 6 cut(s) 112, 282, 466, 543, 758, 976
FbaI TGATCA 1 cut(s) 540
Fnu4HI GCNGC 2 cut(s) 373, 1264
FokI GGATG 5 cut(s) 212, 229, 263, 1074, 1167
Fsp4HI GCNGC 2 cut(s) 373, 1264
FspBI CTAG 3 cut(s) 192, 657, 1040
GlaI GCGC 1 cut(s) 719
GluI GCNGC 2 cut(s) 373, 1264
GsuI CTGGAG 3 cut(s) 224, 689, 1385
HaeII RGCGCY 1 cut(s) 721
HaeIII GGCC 1 cut(s) 1166
HapII CCGG 1 cut(s) 369
HhaI GCGC 1 cut(s) 720
Hin1II CATG 6 cut(s) 116, 286, 470, 547, 762, 980
Hin6I GCGC 1 cut(s) 718
HinP1I GCGC 1 cut(s) 718
HincII GTYRAC 1 cut(s) 1033
HindII GTYRAC 1 cut(s) 1033
HindIII AAGCTT 1 cut(s) 315
HinfI GANTC 5 cut(s) 488, 509, 605, 944, 1424
HpaII CCGG 1 cut(s) 369
HphI GGTGA 4 cut(s) 637, 997, 1332, 1381
Hpy166II GTNNAC 3 cut(s) 1033, 1073, 1385
Hpy188I TCNGA 3 cut(s) 246, 508, 1296
Hpy8I GTNNAC 3 cut(s) 1033, 1073, 1385
HpyCH4III ACNGT 4 cut(s) 31, 144, 1344, 1484
HpyCH4IV ACGT 3 cut(s) 52, 392, 702
HpyF10VI GCNNNNNNNGC 6 cut(s) 176, 454, 773, 1079, 1172, 1448
HpyF3I CTNAG 1 cut(s) 933
HpySE526I ACGT 3 cut(s) 52, 392, 702
Hsp92II CATG 6 cut(s) 116, 286, 470, 547, 762, 980
HspAI GCGC 1 cut(s) 718
Ksp22I TGATCA 1 cut(s) 540
Kzo9I GATC 3 cut(s) 540, 987, 1291
LpnPI CCDG 7 cut(s) 32, 188, 382, 437, 653, 1145, 1349
Lsp1109I GCAGC 2 cut(s) 359, 1250
LweI GCATC 3 cut(s) 13, 1365, 1480
MaeI CTAG 3 cut(s) 192, 657, 1040
MaeII ACGT 3 cut(s) 52, 392, 702
MaeIII GTNAC 5 cut(s) 360, 590, 625, 710, 972
MalI GATC 3 cut(s) 542, 989, 1293
MboI GATC 3 cut(s) 540, 987, 1291
MboII GAAGA 6 cut(s) 257, 313, 716, 908, 1209, 1329
MflI RGATCY 1 cut(s) 1291
MhlI GDGCHC 3 cut(s) 344, 803, 1075
MlyI GAGTC 2 cut(s) 518, 599
Mph1103I ATGCAT 2 cut(s) 6, 1495
MroXI GAANNNNTTC 1 cut(s) 1331
MseI TTAA 6 cut(s) 60, 275, 869, 1010, 1055, 1149
MspI CCGG 1 cut(s) 369
MwoI GCNNNNNNNGC 6 cut(s) 176, 454, 773, 1079, 1172, 1448
NcoI CCATGG 1 cut(s) 112
NdeII GATC 3 cut(s) 540, 987, 1291
NlaIII CATG 6 cut(s) 116, 286, 470, 547, 762, 980
NlaIV GGNNCC 1 cut(s) 1165
NmuCI GTSAC 4 cut(s) 360, 625, 710, 972
NsiI ATGCAT 2 cut(s) 6, 1495
NspV TTCGAA 1 cut(s) 291
PaeR7I CTCGAG 1 cut(s) 416
PagI TCATGA 2 cut(s) 466, 543
PdmI GAANNNNTTC 1 cut(s) 1331
PfeI GAWTC 3 cut(s) 488, 944, 1424
PkrI GCNGC 2 cut(s) 374, 1265
PleI GAGTC 2 cut(s) 517, 599
PmaCI CACGTG 1 cut(s) 393
PmlI CACGTG 1 cut(s) 393
PpsI GAGTC 2 cut(s) 517, 599
Ppu21I YACGTR 1 cut(s) 393
PshBI ATTAAT 1 cut(s) 60
Psp1406I AACGTT 1 cut(s) 702
PspCI CACGTG 1 cut(s) 393
PspN4I GGNNCC 1 cut(s) 1165
PspPI GGNCC 1 cut(s) 1164
PsuI RGATCY 1 cut(s) 1291
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
SaqAI TTAA 6 cut(s) 60, 275, 869, 1010, 1055, 1149
SatI GCNGC 2 cut(s) 373, 1264
Sau3AI GATC 3 cut(s) 540, 987, 1291
Sau96I GGNCC 1 cut(s) 1164
SchI GAGTC 2 cut(s) 518, 599
SduI GDGCHC 3 cut(s) 344, 803, 1075
SfaNI GCATC 3 cut(s) 13, 1365, 1480
Sfr274I CTCGAG 1 cut(s) 416
SfuI TTCGAA 1 cut(s) 291
SlaI CTCGAG 1 cut(s) 416
SmlI CTYRAG 3 cut(s) 416, 449, 875
SmoI CTYRAG 3 cut(s) 416, 449, 875
SpeI ACTAGT 2 cut(s) 191, 656
SsiI CCGC 1 cut(s) 965
SspMI CTAG 3 cut(s) 192, 657, 1040
StyI CCWWGG 4 cut(s) 112, 155, 882, 1039
TaaI ACNGT 4 cut(s) 31, 144, 1344, 1484
TaiI ACGT 3 cut(s) 55, 395, 705
TaqI TCGA 5 cut(s) 19, 291, 417, 810, 1413
TfiI GAWTC 3 cut(s) 488, 944, 1424
Tru1I TTAA 6 cut(s) 60, 275, 869, 1010, 1055, 1149
Tru9I TTAA 6 cut(s) 60, 275, 869, 1010, 1055, 1149
TscAI CASTG 1 cut(s) 369
TseFI GTSAC 4 cut(s) 360, 625, 710, 972
TseI GCWGC 2 cut(s) 372, 1263
Tsp45I GTSAC 4 cut(s) 360, 625, 710, 972
TspDTI ATGAA 5 cut(s) 455, 560, 747, 942, 993
TspGWI ACGGA 2 cut(s) 960, 1074
TspRI CASTG 1 cut(s) 369
VneI GTGCAC 1 cut(s) 1071
VspI ATTAAT 1 cut(s) 60
XapI RAATTY 5 cut(s) 647, 1139, 1253, 1331, 1353
XhoI CTCGAG 1 cut(s) 416
XmaJI CCTAGG 1 cut(s) 1039
XmnI GAANNNNTTC 1 cut(s) 1331
XspI CTAG 3 cut(s) 192, 657, 1040
Zsp2I ATGCAT 2 cut(s) 6, 1495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.