Rh4AG393300

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004540
Physical Location & Seq
Reverse (-)
69923793 .. 69925328
1536 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG393300.1

Sequence Viewer

Length: 1446 bp
ATGATGGATCGATGCACCAATATCGATGAAAAGGAAAACCAAATACAGATAGATCTGTCGGCCGTCAACACTCACATGTCTGACTTACCCGATCTTGTTATTGACATAATCCTTGGACTCATTCCTACTAAAGCTGCGTTCCAGGTGAGCTTTCTTTGCAAGCAACGGCAAGATGTAGTCTCCTCATTCCCTGTACTCAATTTCAACGAGGATGATGATTTTGACCGCCACAACCTTAATCGTTACCGTCACTACAAACATCAACACAAAAGGTTCATCAATTTCTTGAAGGGCTATTTAGATTATCGTAAAAAGAAGGACCAGAAAGAGCTCCTGAATAAATTAAGTCTTCACATGAGGATGTTTTCGCGTCGAGATTCATCTACCATAACCGAGTGGTTGAAATATGCATGTGAGAGAGGCCTCAAAGAGTTGGATATCAGTCCTAAAATGAGTGTTGACCCAACGAGTTGGATTGTCGGTGAGCGCCCGCCGGAAAGTCTCTACCTCTTCTACATCTTGCCTTGGGTGGCGATTGCTGGTGCAAAATCATCCTTAACTTGTCTAAAGTTGGAGTGTGTGAGAGTTCCGCATCTGCTGCATGTGAGAGTTCCGCACGCTTATGAGCGACTTTTTCCGTCCTTGAAAACTCTATCCCTGAAAACTTCACGGGTTGTTGATCTCGATTCTCTTCTATTTGAGTGCCCATCCATGGAGTACCTGTCATTAACTCAATGTTCGATTGGAAACTCCGAGTTTTATGTTCAATGCTCAAGTCTTAAGTCCTTGGAAATTAAGTATTGCCGTCTTCATGTTATTCGAGTTGATCAAGCCTTCAATCTTGAATCTTTTACATTTCTCTCACCGCCGACATATTCAAGATGTGAAAAGATGATCTTGAAGAATGCCTTCAACTTGAAACATATCAACATTTGTGTTGATTACCTCCGGGAATTTTCTTTATTGGGAAGCCATCGTGAACTAGAGGCCACAATTGACCGATATGTCCATTTGTTCGAGTTCTTTGATGGTTATTTGAATGCCAACGTTTCTGTTAAAGCCTATGAGGCCACAATTTTAGTTGGGGAACTTTTGGACCAAGAATGGTTCTCGTCATATTCGACACATTTTCCGAAGCTGATGACCTTTCTTAGAAAATTTAGAAGCTGCTCGAAAATAAACCTCTACGTTCAGGCTCTCATCATTCCAAAGAATTATAGAAAGATGACCTCCGTTCCACCATTGCCGGAAACTTTCACTTTGCAGGCATGGATGTCGAATCCACCACTAGTGGGAAGTAAAGTCTATTTGGAAATGGAGGACTCCTTGCAATGGATGGCACCACTGGCACAGATATTGATCATGCCGGTGGACGTTGAAGAAGATAGAAAGAAAGTATACGAGCGAGCTAGTCCTGTAGGACATGGACGAATGAGAATGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

481

Amino Acids

56.18

Weight (kDa)

8.45

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 183 - 309 3.9e-09 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1004
AccB1I GGYRCC 1 cut(s) 1339
AccI GTMKAC 1 cut(s) 1398
AccII CGCG 1 cut(s) 370
AciI CCGC 5 cut(s) 226, 491, 590, 614, 866
AclI AACGTT 1 cut(s) 1047
AclWI GGATC 1 cut(s) 15
AcoI YGGCCR 1 cut(s) 60
AcsI RAATTY 2 cut(s) 953, 1157
AfaI GTAC 2 cut(s) 195, 719
AfiI CCNNNNNNNGG 3 cut(s) 712, 1292, 1332
AflII CTTAAG 1 cut(s) 779
AflIII ACRYGT 1 cut(s) 75
AhlI ACTAGT 1 cut(s) 1288
AjnI CCWGG 1 cut(s) 141
AluBI AGCT 6 cut(s) 134, 150, 331, 1138, 1167, 1409
AluI AGCT 6 cut(s) 134, 150, 331, 1138, 1167, 1409
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 2 cut(s) 184, 506
AlwI GGATC 1 cut(s) 15
AoxI GGCC 4 cut(s) 60, 421, 987, 1068
ApeKI GCWGC 3 cut(s) 134, 598, 1167
ApoI RAATTY 2 cut(s) 953, 1157
Asp700I GAANNNNTTC 1 cut(s) 908
AspLEI GCGC 1 cut(s) 489
AspS9I GGNCC 2 cut(s) 319, 1096
AsuC2I CCSGG 1 cut(s) 950
AsuHPI GGTGA 3 cut(s) 157, 494, 855
AvaII GGWCC 2 cut(s) 319, 1096
BaeGI GKGCMC 1 cut(s) 707
BanI GGYRCC 1 cut(s) 1339
BanII GRGCYC 1 cut(s) 333
BbsI GAAGAC 2 cut(s) 341, 800
Bbv12I GWGCWC 1 cut(s) 333
BbvI GCAGC 3 cut(s) 121, 585, 1154
BccI CCATC 4 cut(s) 715, 981, 1022, 1330
BceAI ACGGC 3 cut(s) 47, 182, 789
BcgI CGANNNNNNTGC 4 cut(s) 4, 38, 1257, 1291
BciT130I CCWGG 1 cut(s) 143
BclI TGATCA 2 cut(s) 826, 1359
BcnI CCSGG 1 cut(s) 950
BcoDI GTCTC 2 cut(s) 184, 506
BcuI ACTAGT 1 cut(s) 1288
BfaI CTAG 3 cut(s) 983, 1289, 1410
BfmI CTRYAG 1 cut(s) 1416
BfoI RGCGCY 1 cut(s) 490
BfrI CTTAAG 1 cut(s) 779
BglI GCCNNNNNGGC 1 cut(s) 1067
BglII AGATCT 1 cut(s) 52
BisI GCNGC 3 cut(s) 135, 599, 1168
BlsI GCNGC 3 cut(s) 136, 600, 1169
Bme1390I CCNGG 2 cut(s) 143, 950
Bme18I GGWCC 2 cut(s) 319, 1096
BmgT120I GGNCC 2 cut(s) 319, 1096
BmiI GGNNCC 1 cut(s) 1341
BmrFI CCNGG 2 cut(s) 143, 950
BmsI GCATC 2 cut(s) 2, 601
BpiI GAAGAC 2 cut(s) 341, 800
BpuEI CTTGAG 1 cut(s) 757
BpuMI CCSGG 1 cut(s) 950
Bsa29I ATCGAT 2 cut(s) 10, 24
BsaBI GATNNNNATC 1 cut(s) 1358
BsaJI CCNNGG 4 cut(s) 112, 524, 711, 786
BsaXI ACNNNNNCTCC 2 cut(s) 707, 737
Bsc4I CCNNNNNNNGG 3 cut(s) 712, 1292, 1332
Bse118I RCCGGY 1 cut(s) 1366
Bse1I ACTGG 1 cut(s) 1350
Bse3DI GCAATG 2 cut(s) 1241, 1337
Bse8I GATNNNNATC 1 cut(s) 1358
BseBI CCWGG 1 cut(s) 143
BseCI ATCGAT 2 cut(s) 10, 24
BseDI CCNNGG 4 cut(s) 112, 524, 711, 786
BseGI GGATG 6 cut(s) 217, 366, 551, 707, 1278, 1341
BseJI GATNNNNATC 1 cut(s) 1358
BseLI CCNNNNNNNGG 3 cut(s) 712, 1292, 1332
BseMI GCAATG 2 cut(s) 1241, 1337
BseNI ACTGG 1 cut(s) 1350
BseRI GAGGAG 1 cut(s) 172
BseSI GKGCMC 1 cut(s) 707
BseX3I CGGCCG 1 cut(s) 60
BseXI GCAGC 3 cut(s) 121, 585, 1154
Bsh1236I CGCG 1 cut(s) 370
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 4 cut(s) 62, 423, 989, 1070
BshNI GGYRCC 1 cut(s) 1339
BshVI ATCGAT 2 cut(s) 10, 24
BsiEI CGRYCG 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 333
BsiSI CCGG 4 cut(s) 494, 949, 1247, 1367
BslI CCNNNNNNNGG 3 cut(s) 712, 1292, 1332
BsmAI GTCTC 2 cut(s) 184, 506
BsmI GAATGC 2 cut(s) 910, 1045
BsnI GGCC 4 cut(s) 62, 423, 989, 1070
Bsp1286I GDGCHC 2 cut(s) 333, 707
Bsp143I GATC 7 cut(s) 7, 52, 91, 679, 826, 894, 1359
Bsp19I CCATGG 1 cut(s) 711
BspACI CCGC 5 cut(s) 226, 491, 590, 614, 866
BspANI GGCC 4 cut(s) 62, 423, 989, 1070
BspDI ATCGAT 2 cut(s) 10, 24
BspFNI CGCG 1 cut(s) 370
BspLI GGNNCC 1 cut(s) 1341
BspPI GGATC 1 cut(s) 15
BspT107I GGYRCC 1 cut(s) 1339
BspTI CTTAAG 1 cut(s) 779
BsrDI GCAATG 2 cut(s) 1241, 1337
BsrFI RCCGGY 1 cut(s) 1366
BsrI ACTGG 1 cut(s) 1350
BssAI RCCGGY 1 cut(s) 1366
BssECI CCNNGG 4 cut(s) 112, 524, 711, 786
BssMI GATC 7 cut(s) 7, 52, 91, 679, 826, 894, 1359
BssNAI GTATAC 1 cut(s) 1399
BssT1I CCWWGG 4 cut(s) 112, 524, 711, 786
Bst1107I GTATAC 1 cut(s) 1399
Bst2UI CCWGG 1 cut(s) 143
Bst4CI ACNGT 1 cut(s) 248
Bst6I CTCTTC 2 cut(s) 515, 696
BstAFI CTTAAG 1 cut(s) 779
BstAPI GCANNNNNTGC 1 cut(s) 598
BstC8I GCNNGC 5 cut(s) 161, 491, 618, 1266, 1407
BstDEI CTNAG 1 cut(s) 1151
BstDSI CCRYGG 1 cut(s) 711
BstF5I GGATG 6 cut(s) 217, 366, 551, 707, 1278, 1341
BstFNI CGCG 1 cut(s) 370
BstH2I RGCGCY 1 cut(s) 490
BstHHI GCGC 1 cut(s) 489
BstKTI GATC 7 cut(s) 10, 55, 94, 682, 829, 897, 1362
BstMAI GTCTC 2 cut(s) 184, 506
BstMBI GATC 7 cut(s) 7, 52, 91, 679, 826, 894, 1359
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 4 cut(s) 156, 598, 1067, 1346
BstNI CCWGG 1 cut(s) 143
BstNSI RCATGY 3 cut(s) 79, 414, 605
BstSCI CCNGG 2 cut(s) 141, 948
BstSFI CTRYAG 1 cut(s) 1416
BstSLI GKGCMC 1 cut(s) 707
BstUI CGCG 1 cut(s) 370
BstV1I GCAGC 3 cut(s) 121, 585, 1154
BstV2I GAAGAC 2 cut(s) 341, 800
BstX2I RGATCY 1 cut(s) 52
BstXI CCANNNNNNTGG 1 cut(s) 471
BstYI RGATCY 1 cut(s) 52
BstZ17I GTATAC 1 cut(s) 1399
BstZI CGGCCG 1 cut(s) 60
Bsu15I ATCGAT 2 cut(s) 10, 24
BsuRI GGCC 4 cut(s) 62, 423, 989, 1070
BsuTUI ATCGAT 2 cut(s) 10, 24
BtgI CCRYGG 1 cut(s) 711
BtsCI GGATG 6 cut(s) 217, 366, 551, 707, 1278, 1341
BtsIMutI CAGTG 1 cut(s) 1343
Cac8I GCNNGC 5 cut(s) 161, 491, 618, 1266, 1407
CfoI GCGC 1 cut(s) 489
Cfr10I RCCGGY 1 cut(s) 1366
Cfr13I GGNCC 2 cut(s) 319, 1096
ClaI ATCGAT 2 cut(s) 10, 24
CseI GACGC 1 cut(s) 359
Csp6I GTAC 2 cut(s) 194, 718
CviAII CATG 9 cut(s) 76, 355, 411, 602, 712, 812, 1269, 1363, 1424
CviQI GTAC 2 cut(s) 194, 718
DdeI CTNAG 1 cut(s) 1151
DpnI GATC 7 cut(s) 9, 54, 93, 681, 828, 896, 1361
DpnII GATC 7 cut(s) 7, 52, 91, 679, 826, 894, 1359
DrdI GACNNNNNNGTC 1 cut(s) 1004
DseDI GACNNNNNNGTC 1 cut(s) 1004
EaeI YGGCCR 1 cut(s) 60
EagI CGGCCG 1 cut(s) 60
Eam1104I CTCTTC 2 cut(s) 515, 696
EarI CTCTTC 2 cut(s) 515, 696
Ecl136II GAGCTC 1 cut(s) 331
EclXI CGGCCG 1 cut(s) 60
Eco130I CCWWGG 4 cut(s) 112, 524, 711, 786
Eco147I AGGCCT 1 cut(s) 423
Eco24I GRGCYC 1 cut(s) 333
Eco32I GATATC 1 cut(s) 439
Eco47I GGWCC 2 cut(s) 319, 1096
Eco52I CGGCCG 1 cut(s) 60
Eco53kI GAGCTC 1 cut(s) 331
EcoICRI GAGCTC 1 cut(s) 331
EcoRII CCWGG 1 cut(s) 141
EcoRV GATATC 1 cut(s) 439
EcoT14I CCWWGG 4 cut(s) 112, 524, 711, 786
EcoT22I ATGCAT 1 cut(s) 412
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 4 cut(s) 112, 524, 711, 786
FaeI CATG 9 cut(s) 79, 358, 414, 605, 715, 815, 1272, 1366, 1427
FalI AAGNNNNNCTT 4 cut(s) 881, 913, 893, 925
FatI CATG 9 cut(s) 75, 354, 410, 601, 711, 811, 1268, 1362, 1423
FauI CCCGC 1 cut(s) 498
FbaI TGATCA 2 cut(s) 826, 1359
FblI GTMKAC 1 cut(s) 1398
Fnu4HI GCNGC 3 cut(s) 135, 599, 1168
FokI GGATG 6 cut(s) 224, 373, 538, 694, 1285, 1348
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 3 cut(s) 135, 599, 1168
FspBI CTAG 3 cut(s) 983, 1289, 1410
GlaI GCGC 1 cut(s) 488
GluI GCNGC 3 cut(s) 135, 599, 1168
HaeII RGCGCY 1 cut(s) 490
HaeIII GGCC 4 cut(s) 62, 423, 989, 1070
HapII CCGG 4 cut(s) 494, 949, 1247, 1367
HgaI GACGC 1 cut(s) 359
HhaI GCGC 1 cut(s) 489
Hin1II CATG 9 cut(s) 79, 358, 414, 605, 715, 815, 1272, 1366, 1427
Hin6I GCGC 1 cut(s) 487
HinP1I GCGC 1 cut(s) 487
HincII GTYRAC 2 cut(s) 67, 460
HindII GTYRAC 2 cut(s) 67, 460
HinfI GANTC 6 cut(s) 117, 377, 686, 845, 1279, 1322
HpaII CCGG 4 cut(s) 494, 949, 1247, 1367
HphI GGTGA 3 cut(s) 157, 494, 855
Hpy166II GTNNAC 5 cut(s) 67, 460, 980, 1372, 1399
Hpy188I TCNGA 3 cut(s) 82, 754, 1134
Hpy188III TCNNGA 8 cut(s) 286, 334, 374, 683, 842, 879, 898, 977
Hpy8I GTNNAC 5 cut(s) 67, 460, 980, 1372, 1399
Hpy99I CGWCG 1 cut(s) 375
HpyAV CCTTC 4 cut(s) 283, 310, 844, 919
HpyCH4III ACNGT 1 cut(s) 248
HpyCH4IV ACGT 3 cut(s) 1047, 1188, 1374
HpyCH4V TGCA 7 cut(s) 15, 159, 410, 545, 601, 1264, 1330
HpyF10VI GCNNNNNNNGC 4 cut(s) 156, 598, 1067, 1346
HpyF3I CTNAG 1 cut(s) 1151
HpySE526I ACGT 3 cut(s) 1047, 1188, 1374
Hsp92II CATG 9 cut(s) 79, 358, 414, 605, 715, 815, 1272, 1366, 1427
HspAI GCGC 1 cut(s) 487
Ksp22I TGATCA 2 cut(s) 826, 1359
Kzo9I GATC 7 cut(s) 7, 52, 91, 679, 826, 894, 1359
LmnI GCTCC 1 cut(s) 336
Lsp1109I GCAGC 3 cut(s) 121, 585, 1154
LweI GCATC 2 cut(s) 2, 601
MaeI CTAG 3 cut(s) 983, 1289, 1410
MaeII ACGT 3 cut(s) 1047, 1188, 1374
MaeIII GTNAC 2 cut(s) 242, 248
MalI GATC 7 cut(s) 9, 54, 93, 681, 828, 896, 1361
MboI GATC 7 cut(s) 7, 52, 91, 679, 826, 894, 1359
MboII GAAGA 7 cut(s) 341, 502, 683, 800, 913, 1391, 1394
MfeI CAATTG 1 cut(s) 993
MflI RGATCY 1 cut(s) 52
MhlI GDGCHC 2 cut(s) 333, 707
MluCI AATT 9 cut(s) 199, 280, 341, 792, 953, 993, 1074, 1157, 1213
MlyI GAGTC 2 cut(s) 111, 1316
MmeI TCCRAC 3 cut(s) 414, 452, 552
Mph1103I ATGCAT 1 cut(s) 412
MroXI GAANNNNTTC 1 cut(s) 908
MseI TTAA 7 cut(s) 237, 344, 557, 728, 780, 795, 1056
MslI CAYNNNNRTG 4 cut(s) 74, 359, 621, 1367
MspCI CTTAAG 1 cut(s) 779
MspI CCGG 4 cut(s) 494, 949, 1247, 1367
MspR9I CCNGG 2 cut(s) 143, 950
MunI CAATTG 1 cut(s) 993
Mva1269I GAATGC 2 cut(s) 910, 1045
MvaI CCWGG 1 cut(s) 143
MvnI CGCG 1 cut(s) 370
MwoI GCNNNNNNNGC 4 cut(s) 156, 598, 1067, 1346
NciI CCSGG 1 cut(s) 950
NcoI CCATGG 1 cut(s) 711
NdeII GATC 7 cut(s) 7, 52, 91, 679, 826, 894, 1359
NlaIII CATG 9 cut(s) 79, 358, 414, 605, 715, 815, 1272, 1366, 1427
NlaIV GGNNCC 1 cut(s) 1341
NmuCI GTSAC 1 cut(s) 248
NsiI ATGCAT 1 cut(s) 412
NspI RCATGY 3 cut(s) 79, 414, 605
PceI AGGCCT 1 cut(s) 423
PciI ACATGT 1 cut(s) 75
PcsI WCGNNNNNNNCGW 1 cut(s) 1118
PctI GAATGC 2 cut(s) 910, 1045
PdmI GAANNNNTTC 1 cut(s) 908
PfeI GAWTC 4 cut(s) 377, 686, 845, 1279
PfoI TCCNGGA 1 cut(s) 948
PkrI GCNGC 3 cut(s) 136, 600, 1169
PleI GAGTC 2 cut(s) 111, 1316
PpsI GAGTC 2 cut(s) 111, 1316
PscI ACATGT 1 cut(s) 75
Psp124BI GAGCTC 1 cut(s) 333
Psp1406I AACGTT 1 cut(s) 1047
Psp6I CCWGG 1 cut(s) 141
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 1 cut(s) 1341
PspPI GGNCC 2 cut(s) 319, 1096
PsuI RGATCY 1 cut(s) 52
RsaI GTAC 2 cut(s) 195, 719
RsaNI GTAC 2 cut(s) 194, 718
RseI CAYNNNNRTG 4 cut(s) 74, 359, 621, 1367
SacI GAGCTC 1 cut(s) 333
SaqAI TTAA 7 cut(s) 237, 344, 557, 728, 780, 795, 1056
SatI GCNGC 3 cut(s) 135, 599, 1168
Sau3AI GATC 7 cut(s) 7, 52, 91, 679, 826, 894, 1359
Sau96I GGNCC 2 cut(s) 319, 1096
SchI GAGTC 2 cut(s) 111, 1316
ScrFI CCNGG 2 cut(s) 143, 950
SduI GDGCHC 2 cut(s) 333, 707
SfaNI GCATC 2 cut(s) 2, 601
SfcI CTRYAG 1 cut(s) 1416
SinI GGWCC 2 cut(s) 319, 1096
SmiMI CAYNNNNRTG 4 cut(s) 74, 359, 621, 1367
SmlI CTYRAG 2 cut(s) 772, 779
SmoI CTYRAG 2 cut(s) 772, 779
SpeI ACTAGT 1 cut(s) 1288
Sse9I AATT 9 cut(s) 199, 280, 341, 792, 953, 993, 1074, 1157, 1213
SseBI AGGCCT 1 cut(s) 423
SsiI CCGC 5 cut(s) 226, 491, 590, 614, 866
SspMI CTAG 3 cut(s) 983, 1289, 1410
SstI GAGCTC 1 cut(s) 333
StuI AGGCCT 1 cut(s) 423
StyD4I CCNGG 2 cut(s) 141, 948
StyI CCWWGG 4 cut(s) 112, 524, 711, 786
TaaI ACNGT 1 cut(s) 248
TaiI ACGT 3 cut(s) 1050, 1191, 1377
TaqII GACCGA 1 cut(s) 1014
TasI AATT 9 cut(s) 199, 280, 341, 792, 953, 993, 1074, 1157, 1213
TatI WGTACW 1 cut(s) 193
TfiI GAWTC 4 cut(s) 377, 686, 845, 1279
Tru1I TTAA 7 cut(s) 237, 344, 557, 728, 780, 795, 1056
Tru9I TTAA 7 cut(s) 237, 344, 557, 728, 780, 795, 1056
TscAI CASTG 1 cut(s) 1350
TseFI GTSAC 1 cut(s) 248
TseI GCWGC 3 cut(s) 134, 598, 1167
Tsp45I GTSAC 1 cut(s) 248
TspDTI ATGAA 4 cut(s) 42, 265, 369, 800
TspGWI ACGGA 2 cut(s) 627, 1222
TspRI CASTG 1 cut(s) 1350
Vha464I CTTAAG 1 cut(s) 779
VpaK11BI GGWCC 2 cut(s) 319, 1096
XapI RAATTY 2 cut(s) 953, 1157
XceI RCATGY 3 cut(s) 79, 414, 605
XmiI GTMKAC 1 cut(s) 1398
XmnI GAANNNNTTC 1 cut(s) 908
XspI CTAG 3 cut(s) 983, 1289, 1410
Zsp2I ATGCAT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.