Rh4AG397300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004540
Physical Location & Seq
Forward (+)
70217638 .. 70218444
807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG397300.1

Sequence Viewer

Length: 807 bp
ATGTCCTTCACAGCTGTGACGTTCCATGGCTATAGTTTCTTCCCTGTACTATGGGAGTCCTCTTCCATCGAGTACTTGTCATTAACTGCATGTTCTTTTGAGATCCATGGCTATAGTTACGTTGAAAGTTCGAGTCTCAAGTGCCTGGAAGTTAAGTATTGTCATGGGCTTAAGTTTATTATTGTTCACGAAGCTATGAATCTCGAGTCTCTTACATTTGTTTCACCGCCACCACCATATTCAAAATGTACAAGGGTGACGGTGAATAATACCCCCAACTTGAGAAAGATCGATATTTGTGTTGATGAACTCGGGGAATTATATTTATGGCAATGCCATCGTGCACTAAAGGCCACCATCAATACCCAAAATCTCAATCAATTCAGATTCTATGGTTCATTGGAAGCCCAAGTTTCTGTTAAAGCATGTTGGAAATCCGTAATTTCAGTTTCGGAACTTTGGGATGAAGAATGGTTCTGTTCAAACTTGACAAATTTGTCTACTCTGATGGACTTCCTTAAAGGATTTGGCTGCTGCAAGAAGGTGTGGCTGTGTACACGTGATTTTAAAGCTCTCATCTTTCCAGAAACTTTTAGGAAGACCACCGGCACCCTCGCTACACCGTTGCCTAAAAGTTGCATTCTGAACGTGGCAATGCTGAATCCTCCAACAGCGCGCAGTAGAGACTACTGGGACTTGAAGGACTCCATGAATTGGATTGCACGTTCTGCACGGATTGTTAGAGATAATCCCCGATGGCTCGTTACGTACTTGGAAGAAGGCGGAAAGCAAGCAAACTCGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

268

Amino Acids

30.69

Weight (kDa)

8.21

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 8 - 103 1.3e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 496
AccB1I GGYRCC 1 cut(s) 608
AccB7I CCANNNNNTGG 1 cut(s) 714
AccI GTMKAC 1 cut(s) 500
AccII CGCG 1 cut(s) 676
AciI CCGC 2 cut(s) 227, 783
AclWI GGATC 1 cut(s) 97
AcsI RAATTY 1 cut(s) 493
AcvI CACGTG 1 cut(s) 560
AfaI GTAC 5 cut(s) 48, 74, 250, 556, 770
AfiI CCNNNNNNNGG 1 cut(s) 714
AflII CTTAAG 1 cut(s) 170
AflIII ACRYGT 1 cut(s) 557
AgsI TTSAA 4 cut(s) 125, 243, 483, 700
AjnI CCWGG 1 cut(s) 144
AleI CACNNNNGTG 1 cut(s) 14
AloI GAACNNNNNNTCC 2 cut(s) 709, 741
AluBI AGCT 3 cut(s) 14, 194, 572
AluI AGCT 3 cut(s) 14, 194, 572
Alw21I GWGCWC 1 cut(s) 346
Alw26I GTCTC 3 cut(s) 140, 213, 678
Alw44I GTGCAC 1 cut(s) 342
AlwI GGATC 1 cut(s) 97
Ama87I CYCGRG 2 cut(s) 203, 311
AoxI GGCC 2 cut(s) 351, 801
ApaLI GTGCAC 1 cut(s) 342
ApeKI GCWGC 2 cut(s) 531, 534
ApoI RAATTY 1 cut(s) 493
AspLEI GCGC 2 cut(s) 676, 678
AsuHPI GGTGA 3 cut(s) 216, 268, 274
AvaI CYCGRG 2 cut(s) 203, 311
BaeGI GKGCMC 1 cut(s) 346
BanI GGYRCC 1 cut(s) 608
BbrPI CACGTG 1 cut(s) 560
BbsI GAAGAC 1 cut(s) 605
Bbv12I GWGCWC 1 cut(s) 346
BbvI GCAGC 2 cut(s) 518, 521
BccI CCATC 5 cut(s) 74, 345, 365, 502, 750
BcgI CGANNNNNNTGC 2 cut(s) 320, 354
BciT130I CCWGG 1 cut(s) 146
BcoDI GTCTC 3 cut(s) 140, 213, 678
BfaI CTAG 1 cut(s) 805
BfmI CTRYAG 2 cut(s) 31, 112
BfrI CTTAAG 1 cut(s) 170
BisI GCNGC 2 cut(s) 532, 535
BlsI GCNGC 2 cut(s) 533, 536
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 1 cut(s) 146
BmeT110I CYCGRG 2 cut(s) 203, 311
BmiI GGNNCC 1 cut(s) 610
BmrFI CCNGG 1 cut(s) 146
BmrI ACTGGG 1 cut(s) 700
BmuI ACTGGG 1 cut(s) 700
BpiI GAAGAC 1 cut(s) 605
BpuEI CTTGAG 2 cut(s) 122, 301
Bsa29I ATCGAT 1 cut(s) 291
BsaAI YACGTR 2 cut(s) 560, 768
BsaJI CCNNGG 2 cut(s) 25, 106
Bsc4I CCNNNNNNNGG 1 cut(s) 714
Bse118I RCCGGY 1 cut(s) 605
Bse1I ACTGG 1 cut(s) 695
Bse3DI GCAATG 2 cut(s) 338, 660
BseBI CCWGG 1 cut(s) 146
BseCI ATCGAT 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 25, 106
BseGI GGATG 1 cut(s) 469
BseLI CCNNNNNNNGG 1 cut(s) 714
BseMI GCAATG 2 cut(s) 338, 660
BseNI ACTGG 1 cut(s) 695
BsePI GCGCGC 1 cut(s) 674
BseSI GKGCMC 1 cut(s) 346
BseXI GCAGC 2 cut(s) 518, 521
BsgI GTGCAG 1 cut(s) 714
Bsh1236I CGCG 1 cut(s) 676
BshFI GGCC 2 cut(s) 353, 803
BshNI GGYRCC 1 cut(s) 608
BshVI ATCGAT 1 cut(s) 291
BsiHKAI GWGCWC 1 cut(s) 346
BsiHKCI CYCGRG 2 cut(s) 203, 311
BsiSI CCGG 1 cut(s) 606
BslFI GGGAC 1 cut(s) 707
BslI CCNNNNNNNGG 1 cut(s) 714
BsmAI GTCTC 3 cut(s) 140, 213, 678
BsmFI GGGAC 1 cut(s) 707
BsmI GAATGC 1 cut(s) 639
BsnI GGCC 2 cut(s) 353, 803
BsoBI CYCGRG 2 cut(s) 203, 311
Bsp1286I GDGCHC 1 cut(s) 346
Bsp1407I TGTACA 2 cut(s) 248, 554
Bsp143I GATC 2 cut(s) 102, 288
Bsp19I CCATGG 2 cut(s) 25, 106
BspACI CCGC 2 cut(s) 227, 783
BspANI GGCC 2 cut(s) 353, 803
BspDI ATCGAT 1 cut(s) 291
BspFNI CGCG 1 cut(s) 676
BspLI GGNNCC 1 cut(s) 610
BspPI GGATC 1 cut(s) 97
BspT107I GGYRCC 1 cut(s) 608
BspTI CTTAAG 1 cut(s) 170
BsrDI GCAATG 2 cut(s) 338, 660
BsrFI RCCGGY 1 cut(s) 605
BsrGI TGTACA 2 cut(s) 248, 554
BsrI ACTGG 1 cut(s) 695
BssAI RCCGGY 1 cut(s) 605
BssECI CCNNGG 2 cut(s) 25, 106
BssHII GCGCGC 1 cut(s) 674
BssMI GATC 2 cut(s) 102, 288
BssT1I CCWWGG 2 cut(s) 25, 106
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 2 cut(s) 262, 624
Bst6I CTCTTC 1 cut(s) 67
BstAFI CTTAAG 1 cut(s) 170
BstAPI GCANNNNNTGC 1 cut(s) 728
BstAUI TGTACA 2 cut(s) 248, 554
BstBAI YACGTR 2 cut(s) 560, 768
BstC8I GCNNGC 2 cut(s) 676, 792
BstDSI CCRYGG 2 cut(s) 25, 106
BstF5I GGATG 1 cut(s) 469
BstFNI CGCG 1 cut(s) 676
BstHHI GCGC 2 cut(s) 676, 678
BstKTI GATC 2 cut(s) 105, 291
BstMAI GTCTC 3 cut(s) 140, 213, 678
BstMBI GATC 2 cut(s) 102, 288
BstMWI GCNNNNNNNGC 3 cut(s) 350, 728, 800
BstNI CCWGG 1 cut(s) 146
BstNSI RCATGY 2 cut(s) 93, 429
BstSCI CCNGG 1 cut(s) 144
BstSFI CTRYAG 2 cut(s) 31, 112
BstSLI GKGCMC 1 cut(s) 346
BstSNI TACGTA 1 cut(s) 768
BstUI CGCG 1 cut(s) 676
BstV1I GCAGC 2 cut(s) 518, 521
BstV2I GAAGAC 1 cut(s) 605
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
Bsu15I ATCGAT 1 cut(s) 291
BsuRI GGCC 2 cut(s) 353, 803
BsuTUI ATCGAT 1 cut(s) 291
BtgI CCRYGG 2 cut(s) 25, 106
BtsCI GGATG 1 cut(s) 469
Cac8I GCNNGC 2 cut(s) 676, 792
CfoI GCGC 2 cut(s) 676, 678
Cfr10I RCCGGY 1 cut(s) 605
ClaI ATCGAT 1 cut(s) 291
Csp6I GTAC 5 cut(s) 47, 73, 249, 555, 769
CviAII CATG 6 cut(s) 26, 90, 107, 164, 426, 709
CviQI GTAC 5 cut(s) 47, 73, 249, 555, 769
DpnI GATC 2 cut(s) 104, 290
DpnII GATC 2 cut(s) 102, 288
DraI TTTAAA 1 cut(s) 568
DrdI GACNNNNNNGTC 1 cut(s) 496
DseDI GACNNNNNNGTC 1 cut(s) 496
Eam1104I CTCTTC 1 cut(s) 67
EarI CTCTTC 1 cut(s) 67
EciI GGCGGA 1 cut(s) 798
Eco105I TACGTA 1 cut(s) 768
Eco130I CCWWGG 2 cut(s) 25, 106
Eco72I CACGTG 1 cut(s) 560
Eco88I CYCGRG 2 cut(s) 203, 311
EcoRII CCWGG 1 cut(s) 144
EcoT14I CCWWGG 2 cut(s) 25, 106
ErhI CCWWGG 2 cut(s) 25, 106
FaeI CATG 6 cut(s) 29, 93, 110, 167, 429, 712
FaqI GGGAC 1 cut(s) 707
FatI CATG 6 cut(s) 25, 89, 106, 163, 425, 708
FblI GTMKAC 1 cut(s) 500
Fnu4HI GCNGC 2 cut(s) 532, 535
FokI GGATG 1 cut(s) 476
Fsp4HI GCNGC 2 cut(s) 532, 535
FspBI CTAG 1 cut(s) 805
GlaI GCGC 2 cut(s) 675, 677
GluI GCNGC 2 cut(s) 532, 535
HaeIII GGCC 2 cut(s) 353, 803
HapII CCGG 1 cut(s) 606
HhaI GCGC 2 cut(s) 676, 678
Hin1II CATG 6 cut(s) 29, 93, 110, 167, 429, 712
Hin6I GCGC 2 cut(s) 674, 676
HinP1I GCGC 2 cut(s) 674, 676
HinfI GANTC 7 cut(s) 56, 133, 199, 206, 387, 661, 704
HpaII CCGG 1 cut(s) 606
HphI GGTGA 3 cut(s) 216, 268, 274
Hpy166II GTNNAC 5 cut(s) 187, 344, 501, 555, 557
Hpy188I TCNGA 4 cut(s) 386, 454, 507, 645
Hpy188III TCNNGA 3 cut(s) 188, 203, 584
Hpy8I GTNNAC 5 cut(s) 187, 344, 501, 555, 557
HpyAV CCTTC 4 cut(s) 16, 535, 694, 773
HpyCH4III ACNGT 2 cut(s) 262, 624
HpyCH4IV ACGT 6 cut(s) 20, 120, 559, 648, 724, 767
HpyCH4V TGCA 6 cut(s) 89, 344, 537, 639, 722, 731
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 728, 800
HpySE526I ACGT 6 cut(s) 20, 120, 559, 648, 724, 767
Hsp92II CATG 6 cut(s) 29, 93, 110, 167, 429, 712
HspAI GCGC 2 cut(s) 674, 676
Kzo9I GATC 2 cut(s) 102, 288
LpnPI CCDG 6 cut(s) 57, 131, 158, 597, 619, 676
Lsp1109I GCAGC 2 cut(s) 518, 521
MaeI CTAG 1 cut(s) 805
MaeII ACGT 6 cut(s) 20, 120, 559, 648, 724, 767
MaeIII GTNAC 4 cut(s) 16, 116, 256, 763
MalI GATC 2 cut(s) 104, 290
MboI GATC 2 cut(s) 102, 288
MboII GAAGA 5 cut(s) 31, 54, 479, 610, 788
MflI RGATCY 1 cut(s) 102
MhlI GDGCHC 1 cut(s) 346
MluCI AATT 5 cut(s) 317, 380, 441, 493, 712
MlyI GAGTC 4 cut(s) 65, 142, 215, 698
MmeI TCCRAC 2 cut(s) 410, 692
MnlI CCTC 3 cut(s) 70, 623, 675
MseI TTAA 6 cut(s) 83, 153, 171, 420, 519, 567
MslI CAYNNNNRTG 1 cut(s) 14
MspA1I CMGCKG 1 cut(s) 14
MspCI CTTAAG 1 cut(s) 170
MspI CCGG 1 cut(s) 606
MspR9I CCNGG 1 cut(s) 146
Mva1269I GAATGC 1 cut(s) 639
MvaI CCWGG 1 cut(s) 146
MvnI CGCG 1 cut(s) 676
MwoI GCNNNNNNNGC 3 cut(s) 350, 728, 800
NcoI CCATGG 2 cut(s) 25, 106
NdeII GATC 2 cut(s) 102, 288
NlaIII CATG 6 cut(s) 29, 93, 110, 167, 429, 712
NlaIV GGNNCC 1 cut(s) 610
NmeAIII GCCGAG 1 cut(s) 779
NmuCI GTSAC 2 cut(s) 16, 256
NspI RCATGY 2 cut(s) 93, 429
OliI CACNNNNGTG 1 cut(s) 14
PaeR7I CTCGAG 1 cut(s) 203
PauI GCGCGC 1 cut(s) 674
PctI GAATGC 1 cut(s) 639
PfeI GAWTC 3 cut(s) 199, 387, 661
PflMI CCANNNNNTGG 1 cut(s) 714
PkrI GCNGC 2 cut(s) 533, 536
PleI GAGTC 4 cut(s) 64, 141, 214, 698
PmaCI CACGTG 1 cut(s) 560
PmlI CACGTG 1 cut(s) 560
PpsI GAGTC 4 cut(s) 64, 141, 214, 698
Ppu21I YACGTR 2 cut(s) 560, 768
Psp6I CCWGG 1 cut(s) 144
PspCI CACGTG 1 cut(s) 560
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 1 cut(s) 610
PsuI RGATCY 1 cut(s) 102
PteI GCGCGC 1 cut(s) 674
PvuII CAGCTG 1 cut(s) 14
RsaI GTAC 5 cut(s) 48, 74, 250, 556, 770
RsaNI GTAC 5 cut(s) 47, 73, 249, 555, 769
RseI CAYNNNNRTG 1 cut(s) 14
SaqAI TTAA 6 cut(s) 83, 153, 171, 420, 519, 567
SatI GCNGC 2 cut(s) 532, 535
Sau3AI GATC 2 cut(s) 102, 288
ScaI AGTACT 1 cut(s) 74
SchI GAGTC 4 cut(s) 65, 142, 215, 698
ScrFI CCNGG 1 cut(s) 146
SduI GDGCHC 1 cut(s) 346
SfcI CTRYAG 2 cut(s) 31, 112
Sfr274I CTCGAG 1 cut(s) 203
SlaI CTCGAG 1 cut(s) 203
SmiMI CAYNNNNRTG 1 cut(s) 14
SmlI CTYRAG 4 cut(s) 137, 170, 203, 280
SmoI CTYRAG 4 cut(s) 137, 170, 203, 280
SnaBI TACGTA 1 cut(s) 768
Sse9I AATT 5 cut(s) 317, 380, 441, 493, 712
SsiI CCGC 2 cut(s) 227, 783
SspMI CTAG 1 cut(s) 805
StyD4I CCNGG 1 cut(s) 144
StyI CCWWGG 2 cut(s) 25, 106
TaaI ACNGT 2 cut(s) 262, 624
TaiI ACGT 6 cut(s) 23, 123, 562, 651, 727, 770
TaqI TCGA 4 cut(s) 69, 131, 204, 291
TasI AATT 5 cut(s) 317, 380, 441, 493, 712
TatI WGTACW 4 cut(s) 46, 72, 248, 554
TfiI GAWTC 3 cut(s) 199, 387, 661
Tru1I TTAA 6 cut(s) 83, 153, 171, 420, 519, 567
Tru9I TTAA 6 cut(s) 83, 153, 171, 420, 519, 567
TseFI GTSAC 2 cut(s) 16, 256
TseI GCWGC 2 cut(s) 531, 534
Tsp45I GTSAC 2 cut(s) 16, 256
TspDTI ATGAA 5 cut(s) 212, 321, 387, 480, 725
TspGWI ACGGA 2 cut(s) 427, 748
Van91I CCANNNNNTGG 1 cut(s) 714
Vha464I CTTAAG 1 cut(s) 170
VneI GTGCAC 1 cut(s) 342
XapI RAATTY 1 cut(s) 493
XceI RCATGY 2 cut(s) 93, 429
XhoI CTCGAG 1 cut(s) 203
XmiI GTMKAC 1 cut(s) 500
XspI CTAG 1 cut(s) 805
ZrmI AGTACT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.