RchiOBHm_Chr4g0442851

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004540
Physical Location & Seq
Forward (+)
64306923 .. 64307423
501 bp
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UTR
Exon/CDS
Intron
PRQ41059

Sequence Viewer

Length: 501 bp
ATGAGAGTTCCGGACATTCGAGCTGAGGTAGACATTGAACATGACCACCCCCTACTCCCCTCTTTGAAAACCATGTCCTTCACAGCTGTGACGTTCCATGGCTTTAGTTTCTTCCCTGTACTATGGGAGTCCTCTTCCATCGAGTACTTGTCATTAACTGCATGTTCTTTTGAGAACCATGACCCTATGTGTTACGTTAAAAGTTCGAGTCTCAAGTGCTTGGAAGTCAAGTACTGTCATGGGCTTAAGTTTATTATTGTTCACGAAGCTATGAATCTCGAATCTCTTACATTTGGTTCACCGCCACCACCATATTCAAAATGTACAAGGGTGACTGTGAATAATATCCCCAACTTGAGAAAGATCGATATTTGTGTTGATGAACTCGATCGGGGAATTATATTTATCGCAATGCCATCGTGCACTAAAGGCCACCATCAATACCCAAAATCTCAATCAATTCAGATTCTATGGTTCATTGGAAGCCCAAGTTTCTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

166

Amino Acids

18.84

Weight (kDa)

6.43

Isoelectric Point (pI)

57.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 11 - 126 1.3e-08 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 30
AccIII TCCGGA 1 cut(s) 10
AciI CCGC 1 cut(s) 302
AfaI GTAC 4 cut(s) 120, 146, 233, 325
AflII CTTAAG 1 cut(s) 245
AgsI TTSAA 3 cut(s) 38, 67, 318
AleI CACNNNNGTG 1 cut(s) 86
AluBI AGCT 3 cut(s) 23, 86, 269
AluI AGCT 3 cut(s) 23, 86, 269
Alw21I GWGCWC 1 cut(s) 425
Alw26I GTCTC 1 cut(s) 215
Alw44I GTGCAC 1 cut(s) 421
Aor13HI TCCGGA 1 cut(s) 10
AoxI GGCC 1 cut(s) 430
ApaLI GTGCAC 1 cut(s) 421
AsuHPI GGTGA 2 cut(s) 291, 343
BaeGI GKGCMC 1 cut(s) 425
Bbv12I GWGCWC 1 cut(s) 425
BbvCI CCTCAGC 1 cut(s) 24
BccI CCATC 3 cut(s) 146, 424, 444
BcgI CGANNNNNNTGC 2 cut(s) 399, 433
BcoDI GTCTC 1 cut(s) 215
BfrI CTTAAG 1 cut(s) 245
BmcAI AGTACT 2 cut(s) 146, 233
Bpu10I CCTNAGC 1 cut(s) 24
BpuEI CTTGAG 2 cut(s) 197, 376
Bsa29I ATCGAT 1 cut(s) 366
BsaJI CCNNGG 1 cut(s) 97
BsaWI WCCGGW 1 cut(s) 10
Bse3DI GCAATG 1 cut(s) 417
BseAI TCCGGA 1 cut(s) 10
BseCI ATCGAT 1 cut(s) 366
BseDI CCNNGG 1 cut(s) 97
BseMI GCAATG 1 cut(s) 417
BseMII CTCAG 1 cut(s) 15
BseSI GKGCMC 1 cut(s) 425
Bsh1285I CGRYCG 1 cut(s) 391
BshFI GGCC 1 cut(s) 432
BshVI ATCGAT 1 cut(s) 366
BsiEI CGRYCG 1 cut(s) 391
BsiHKAI GWGCWC 1 cut(s) 425
BsiSI CCGG 1 cut(s) 11
BsmAI GTCTC 1 cut(s) 215
BsnI GGCC 1 cut(s) 432
Bsp1286I GDGCHC 1 cut(s) 425
Bsp13I TCCGGA 1 cut(s) 10
Bsp1407I TGTACA 1 cut(s) 323
Bsp143I GATC 2 cut(s) 363, 388
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 1 cut(s) 302
BspANI GGCC 1 cut(s) 432
BspCNI CTCAG 1 cut(s) 16
BspDI ATCGAT 1 cut(s) 366
BspEI TCCGGA 1 cut(s) 10
BspTI CTTAAG 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 417
BsrGI TGTACA 1 cut(s) 323
BssECI CCNNGG 1 cut(s) 97
BssMI GATC 2 cut(s) 363, 388
BssT1I CCWWGG 1 cut(s) 97
Bst4CI ACNGT 2 cut(s) 236, 337
Bst6I CTCTTC 1 cut(s) 139
BstAFI CTTAAG 1 cut(s) 245
BstAUI TGTACA 1 cut(s) 323
BstDEI CTNAG 1 cut(s) 24
BstDSI CCRYGG 1 cut(s) 97
BstKTI GATC 2 cut(s) 366, 391
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 2 cut(s) 363, 388
BstMCI CGRYCG 1 cut(s) 391
BstMWI GCNNNNNNNGC 1 cut(s) 429
BstNSI RCATGY 1 cut(s) 165
BstSLI GKGCMC 1 cut(s) 425
Bsu15I ATCGAT 1 cut(s) 366
BsuRI GGCC 1 cut(s) 432
BsuTUI ATCGAT 1 cut(s) 366
BtgI CCRYGG 1 cut(s) 97
ClaI ATCGAT 1 cut(s) 366
Csp6I GTAC 4 cut(s) 119, 145, 232, 324
CviAII CATG 6 cut(s) 41, 73, 98, 162, 179, 239
CviJI RGCY 7 cut(s) 23, 86, 102, 244, 269, 432, 486
CviKI_1 RGCY 7 cut(s) 23, 86, 102, 244, 269, 432, 486
CviQI GTAC 4 cut(s) 119, 145, 232, 324
DdeI CTNAG 1 cut(s) 24
DpnI GATC 2 cut(s) 365, 390
DpnII GATC 2 cut(s) 363, 388
Eam1104I CTCTTC 1 cut(s) 139
EarI CTCTTC 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 97
EcoT14I CCWWGG 1 cut(s) 97
ErhI CCWWGG 1 cut(s) 97
FaeI CATG 6 cut(s) 44, 76, 101, 165, 182, 242
FatI CATG 6 cut(s) 40, 72, 97, 161, 178, 238
FblI GTMKAC 1 cut(s) 30
HaeIII GGCC 1 cut(s) 432
HapII CCGG 1 cut(s) 11
Hin1II CATG 6 cut(s) 44, 76, 101, 165, 182, 242
HinfI GANTC 5 cut(s) 128, 208, 274, 281, 466
HpaII CCGG 1 cut(s) 11
HphI GGTGA 2 cut(s) 291, 343
Hpy166II GTNNAC 4 cut(s) 31, 262, 299, 423
Hpy188I TCNGA 1 cut(s) 465
Hpy188III TCNNGA 3 cut(s) 11, 263, 278
Hpy8I GTNNAC 4 cut(s) 31, 262, 299, 423
HpyAV CCTTC 1 cut(s) 88
HpyCH4III ACNGT 2 cut(s) 236, 337
HpyCH4IV ACGT 2 cut(s) 92, 195
HpyCH4V TGCA 2 cut(s) 161, 423
HpyF10VI GCNNNNNNNGC 1 cut(s) 429
HpyF3I CTNAG 1 cut(s) 24
HpySE526I ACGT 2 cut(s) 92, 195
Hsp92II CATG 6 cut(s) 44, 76, 101, 165, 182, 242
Kpn2I TCCGGA 1 cut(s) 10
Kzo9I GATC 2 cut(s) 363, 388
LpnPI CCDG 2 cut(s) 24, 129
MaeII ACGT 2 cut(s) 92, 195
MaeIII GTNAC 3 cut(s) 88, 191, 331
MalI GATC 2 cut(s) 365, 390
MboI GATC 2 cut(s) 363, 388
MboII GAAGA 2 cut(s) 103, 126
MhlI GDGCHC 1 cut(s) 425
MluCI AATT 2 cut(s) 396, 459
MlyI GAGTC 2 cut(s) 137, 217
MnlI CCTC 3 cut(s) 19, 70, 142
MroI TCCGGA 1 cut(s) 10
MseI TTAA 4 cut(s) 155, 198, 246, 499
MslI CAYNNNNRTG 1 cut(s) 86
MspA1I CMGCKG 1 cut(s) 86
MspCI CTTAAG 1 cut(s) 245
MspI CCGG 1 cut(s) 11
MwoI GCNNNNNNNGC 1 cut(s) 429
NcoI CCATGG 1 cut(s) 97
NdeII GATC 2 cut(s) 363, 388
NlaIII CATG 6 cut(s) 44, 76, 101, 165, 182, 242
NmuCI GTSAC 2 cut(s) 88, 331
NspI RCATGY 1 cut(s) 165
OliI CACNNNNGTG 1 cut(s) 86
PfeI GAWTC 3 cut(s) 274, 281, 466
Ple19I CGATCG 1 cut(s) 391
PleI GAGTC 2 cut(s) 136, 216
PpsI GAGTC 2 cut(s) 136, 216
PvuI CGATCG 1 cut(s) 391
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 4 cut(s) 120, 146, 233, 325
RsaNI GTAC 4 cut(s) 119, 145, 232, 324
RseI CAYNNNNRTG 1 cut(s) 86
SaqAI TTAA 4 cut(s) 155, 198, 246, 499
Sau3AI GATC 2 cut(s) 363, 388
ScaI AGTACT 2 cut(s) 146, 233
SchI GAGTC 2 cut(s) 137, 217
SduI GDGCHC 1 cut(s) 425
SetI ASST 6 cut(s) 25, 30, 88, 95, 198, 271
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 3 cut(s) 212, 245, 355
SmoI CTYRAG 3 cut(s) 212, 245, 355
Sse9I AATT 2 cut(s) 396, 459
SsiI CCGC 1 cut(s) 302
StyI CCWWGG 1 cut(s) 97
TaaI ACNGT 2 cut(s) 236, 337
TaiI ACGT 2 cut(s) 95, 198
TaqI TCGA 6 cut(s) 19, 141, 206, 279, 366, 387
TasI AATT 2 cut(s) 396, 459
TatI WGTACW 4 cut(s) 118, 144, 231, 323
TfiI GAWTC 3 cut(s) 274, 281, 466
Tru1I TTAA 4 cut(s) 155, 198, 246, 499
Tru9I TTAA 4 cut(s) 155, 198, 246, 499
TseFI GTSAC 2 cut(s) 88, 331
Tsp45I GTSAC 2 cut(s) 88, 331
TspDTI ATGAA 3 cut(s) 287, 396, 466
Vha464I CTTAAG 1 cut(s) 245
VneI GTGCAC 1 cut(s) 421
XceI RCATGY 1 cut(s) 165
XmiI GTMKAC 1 cut(s) 30
ZrmI AGTACT 2 cut(s) 146, 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.