FvH4_4g33501

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004540
Physical Location & Seq
Forward (+)
31723988 .. 31725814
1827 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33501.t1

Sequence Viewer

Length: 1827 bp
ATGCCTGACTTTCACGACTTACCGGACCTTGTTATCAACAAAATAATCGGTCTCCTTCCTTTTAACACTGCCGTGCAGACTACTTTTCTTTCCAAGCAATATGCAGATGCAGTTTCTGCACTAGATTTCAAAGAGTATGAGAATGTTGTCCTTCGCATCTGCGATGGCGTTCCCGTTCCCAACCGATACGATTGCTTTGTTCGACACAACAAGTTCTTCAAATTCTTGAAAGGCTATTTGGAATATCATGAGAAATATGGGAAGAAAGAGGCCATATATACACTAAGGCTCCGAATGAGTATATATTCGCGTCGAGATGTTATTCTCATTGATAGGTGGTTGGATCGTGCAATTCATAGAGGTCTCAAGGAATTGGATATTAGCCCTCATCAATCAAGTACAATCGACGGAATAATAAGGACGGAACTTGAAAAGATGTATCGTTTACCTTGGGTGATCTTTGCTAACAACAATGCACAATCTCTTGCTAGTCTAAACCTGAATTGTGTGAGGATTTCTAACGTTCAAGGTGCGAAACTTCAAAGGGACAAGAAGTGCCTTCTTCCATCCTTAAAAACTATGTCCTTCTCATTGGTATGGTTTGATCCCAATGCTCTCTACTCCCTACTATTAGAGTGCCCTTCCATCAAAAAATTGTCACTGACAGAATGCTTCTTTGAAGACTTCAAGTGTCGTGTTTCAAGCCCTAGTCTCGAGTGCCTGGAACTGATGTATTGTAGTCAGCTTGAGGAGTTTCAAGTTCAGGATGAGACTAAAAATCTTGAATCTTTAATATATTGGTCCCAGGGTGAACCATATTCAACATGTGAAAATGTGATCATGAATAAATCCTTCAAGTTGAAAAGTATCGACATATGGACTAGTAGTGAAAGTGACGACATCTACGCCTACCACTTGCCTTGGGTGAAATTTTTTTCAAATGCAAAATTCCTAACTAGTATGACACTTACCTGCATGAGTATCGCAAACATTAGCGTAAACGTTCAAAGTGATGAGCGCCTTCTTCCCTCTTTGAAAACCTTGTCCGTCAGATCTTCGTGGTTTGCTGACAAAGCTCTCTCCTCATTATTATCGCAGTGCCCTTCCATCAAGTATTTGTCAATAATTGGATGTTGGTTTGAAAACTCAGAGTGTCATGTTTCAAATTCTAGTCTCAAGTCATTAGAAGTAAAGCATTGCGAGCGGCTAAAAGTTCTTCAAGTTGATGATGAAGCAAAGAACCTCGAATCCTTTACATTTGTTCATCCACCACAGCCAGATCCAAACTGTGAAAAGGTGACCTTGAATAATACCTTCAACCTGAAAAATATCTACATTTTTGTGGATGAGCTAGGGGAATTTTCTTTGAACGGATATCATCGTACACTCAAAGCCAAAATCAATGTCCAAAATCTACATCTTTTCGATTTTCTTGGGACTTTGACCGATGGATTTTCTGTTAAAGGATGTCAAGTGGCCAAGATTGCAGTTCGAGAACTTTGGTTCAAAAATTGGTTCGTGCATTACTCTTCACATTTTCCTAGATTGATTGGTTGTCTCGAAGAGTTTGGGTGCTGCAAGAAAATATCTCTGTATAATCAACATATGAGGGCTCTCATCATTCCCAATTATGTAAGGAATGAATTTTCTTCACCGTTGCCTGAAATTCACATTATGGAGGTGAGGATGCCAAATCCACCAAAAAAGGCGAGTAGAGATTATTTCGACTTGATAGACTCCTTGCAATGGATTGCGCCTTCTGCAAAAGTAGAAATATGCAAAAGTGAAACACATCCGTTCGATGTATTATTAGAAGAAGAATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_2

Protein Analysis

609

Amino Acids

70.39

Weight (kDa)

6.61

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 92 - 253 3.9e-09 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 110 - 291 5.2e-11 FBD-associated F-box protein At5g56370, LRR repeats
LRR_At5g56370 PF24758 315 - 442 1.7e-09 FBD-associated F-box protein At5g56370, LRR repeats
LRR_At1g61320_AtMIF1 PF23622 325 - 446 3.2e-06 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 980
AccBSI CCGCTC 1 cut(s) 1204
AccII CGCG 1 cut(s) 310
AciI CCGC 1 cut(s) 1204
AclI AACGTT 2 cut(s) 522, 1002
AclWI GGATC 3 cut(s) 351, 599, 1274
AcoI YGGCCR 1 cut(s) 1476
AcsI RAATTY 7 cut(s) 221, 929, 947, 1165, 1358, 1643, 1665
AfaI GTAC 2 cut(s) 400, 1384
AfiI CCNNNNNNNGG 1 cut(s) 1746
AflIII ACRYGT 1 cut(s) 824
AhlI ACTAGT 2 cut(s) 881, 956
AjnI CCWGG 2 cut(s) 720, 804
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AleI CACNNNNGTG 1 cut(s) 71
AloI GAACNNNNNNTCC 2 cut(s) 1232, 1264
AluBI AGCT 3 cut(s) 745, 1076, 1351
AluI AGCT 3 cut(s) 745, 1076, 1351
Alw26I GTCTC 6 cut(s) 56, 368, 716, 764, 1178, 1562
AlwI GGATC 3 cut(s) 351, 599, 1274
AlwNI CAGNNNCTG 1 cut(s) 116
Ama87I CYCGRG 1 cut(s) 713
AoxI GGCC 2 cut(s) 270, 1476
ApeKI GCWGC 1 cut(s) 1575
ApoI RAATTY 7 cut(s) 221, 929, 947, 1165, 1358, 1643, 1665
ArsI GACNNNNNNTTYG 2 cut(s) 1389, 1421
AspLEI GCGC 2 cut(s) 1020, 1756
AspS9I GGNCC 2 cut(s) 25, 801
AsuHPI GGTGA 6 cut(s) 466, 821, 937, 1309, 1644, 1693
AvaI CYCGRG 1 cut(s) 713
AvaII GGWCC 2 cut(s) 25, 801
BaeGI GKGCMC 2 cut(s) 641, 1103
BalI TGGCCA 1 cut(s) 1478
BanII GRGCYC 1 cut(s) 1615
BbsI GAAGAC 1 cut(s) 687
BbvI GCAGC 1 cut(s) 1562
BccI CCATC 5 cut(s) 158, 574, 653, 1115, 1442
BceAI ACGGC 1 cut(s) 56
BcgI CGANNNNNNTGC 4 cut(s) 174, 208, 964, 998
BciT130I CCWGG 2 cut(s) 722, 806
BclI TGATCA 1 cut(s) 837
BcoDI GTCTC 6 cut(s) 56, 368, 716, 764, 1178, 1562
BcuI ACTAGT 2 cut(s) 881, 956
BfaI CTAG 8 cut(s) 122, 489, 708, 882, 957, 1170, 1352, 1542
BfoI RGCGCY 1 cut(s) 1021
BfuAI ACCTGC 1 cut(s) 980
BglII AGATCT 1 cut(s) 1052
BisI GCNGC 2 cut(s) 1205, 1576
BlsI GCNGC 2 cut(s) 1206, 1577
Bme1390I CCNGG 2 cut(s) 722, 806
Bme18I GGWCC 2 cut(s) 25, 801
BmeT110I CYCGRG 1 cut(s) 713
BmgT120I GGNCC 2 cut(s) 25, 801
BmiI GGNNCC 2 cut(s) 290, 803
BmrFI CCNGG 2 cut(s) 722, 806
BmsI GCATC 3 cut(s) 97, 165, 1677
BpiI GAAGAC 1 cut(s) 687
BpuEI CTTGAG 3 cut(s) 350, 767, 1160
BsaI GGTCTC 2 cut(s) 56, 368
BsaJI CCNNGG 4 cut(s) 449, 804, 805, 920
BsaWI WCCGGW 1 cut(s) 22
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bsc4I CCNNNNNNNGG 1 cut(s) 1746
Bse3DI GCAATG 2 cut(s) 1195, 1751
BseBI CCWGG 2 cut(s) 722, 806
BseDI CCNNGG 4 cut(s) 449, 804, 805, 920
BseGI GGATG 8 cut(s) 566, 772, 1136, 1264, 1351, 1472, 1692, 1792
BseLI CCNNNNNNNGG 1 cut(s) 1746
BseMI GCAATG 2 cut(s) 1195, 1751
BseMII CTCAG 1 cut(s) 1161
BseRI GAGGAG 2 cut(s) 764, 1072
BseSI GKGCMC 2 cut(s) 641, 1103
BseXI GCAGC 1 cut(s) 1562
BsgI GTGCAG 2 cut(s) 95, 102
Bsh1236I CGCG 1 cut(s) 310
BshFI GGCC 2 cut(s) 272, 1478
BsiHKCI CYCGRG 1 cut(s) 713
BsiSI CCGG 1 cut(s) 23
BslFI GGGAC 3 cut(s) 560, 787, 1450
BslI CCNNNNNNNGG 1 cut(s) 1746
BsmAI GTCTC 6 cut(s) 56, 368, 716, 764, 1178, 1562
BsmFI GGGAC 3 cut(s) 560, 787, 1450
BsmI GAATGC 1 cut(s) 674
BsnI GGCC 2 cut(s) 272, 1478
Bso31I GGTCTC 2 cut(s) 56, 368
BsoBI CYCGRG 1 cut(s) 713
Bsp1286I GDGCHC 3 cut(s) 641, 1103, 1615
Bsp143I GATC 6 cut(s) 343, 456, 604, 837, 1052, 1279
BspACI CCGC 1 cut(s) 1204
BspANI GGCC 2 cut(s) 272, 1478
BspCNI CTCAG 1 cut(s) 1160
BspFNI CGCG 1 cut(s) 310
BspHI TCATGA 2 cut(s) 247, 840
BspLI GGNNCC 2 cut(s) 290, 803
BspMI ACCTGC 1 cut(s) 980
BspPI GGATC 3 cut(s) 351, 599, 1274
BspTNI GGTCTC 2 cut(s) 56, 368
BsrBI CCGCTC 1 cut(s) 1204
BsrDI GCAATG 2 cut(s) 1195, 1751
BssECI CCNNGG 4 cut(s) 449, 804, 805, 920
BssMI GATC 6 cut(s) 343, 456, 604, 837, 1052, 1279
BssT1I CCWWGG 2 cut(s) 449, 920
Bst2UI CCWGG 2 cut(s) 722, 806
Bst4CI ACNGT 2 cut(s) 1289, 1656
Bst6I CTCTTC 2 cut(s) 1534, 1557
BstAPI GCANNNNNTGC 1 cut(s) 116
BstC8I GCNNGC 1 cut(s) 1202
BstDEI CTNAG 2 cut(s) 284, 1147
BstEII GGTNACC 1 cut(s) 1297
BstF5I GGATG 8 cut(s) 566, 772, 1136, 1264, 1351, 1472, 1692, 1792
BstFNI CGCG 1 cut(s) 310
BstH2I RGCGCY 1 cut(s) 1021
BstHHI GCGC 2 cut(s) 1020, 1756
BstKTI GATC 6 cut(s) 346, 459, 607, 840, 1055, 1282
BstMAI GTCTC 6 cut(s) 56, 368, 716, 764, 1178, 1562
BstMBI GATC 6 cut(s) 343, 456, 604, 837, 1052, 1279
BstMWI GCNNNNNNNGC 5 cut(s) 116, 1073, 1201, 1484, 1760
BstNI CCWGG 2 cut(s) 722, 806
BstNSI RCATGY 1 cut(s) 828
BstPI GGTNACC 1 cut(s) 1297
BstSCI CCNGG 2 cut(s) 720, 804
BstSLI GKGCMC 2 cut(s) 641, 1103
BstUI CGCG 1 cut(s) 310
BstV1I GCAGC 1 cut(s) 1562
BstV2I GAAGAC 1 cut(s) 687
BstX2I RGATCY 2 cut(s) 1052, 1279
BstYI RGATCY 2 cut(s) 1052, 1279
BsuRI GGCC 2 cut(s) 272, 1478
BtgZI GCGATG 1 cut(s) 177
BtsCI GGATG 8 cut(s) 566, 772, 1136, 1264, 1351, 1472, 1692, 1792
BtsI GCAGTG 2 cut(s) 66, 1103
BtsIMutI CAGTG 3 cut(s) 66, 659, 1103
BveI ACCTGC 1 cut(s) 980
Cac8I GCNNGC 1 cut(s) 1202
CaiI CAGNNNCTG 1 cut(s) 116
CciI TCATGA 2 cut(s) 247, 840
CfoI GCGC 2 cut(s) 1020, 1756
Cfr13I GGNCC 2 cut(s) 25, 801
CseI GACGC 1 cut(s) 299
Csp6I GTAC 2 cut(s) 399, 1383
CspCI CAANNNNNGTGG 2 cut(s) 902, 937
CviAII CATG 5 cut(s) 248, 825, 841, 976, 1157
CviQI GTAC 2 cut(s) 399, 1383
DdeI CTNAG 2 cut(s) 284, 1147
DpnI GATC 6 cut(s) 345, 458, 606, 839, 1054, 1281
DpnII GATC 6 cut(s) 343, 456, 604, 837, 1052, 1279
EaeI YGGCCR 1 cut(s) 1476
Eam1104I CTCTTC 2 cut(s) 1534, 1557
EarI CTCTTC 2 cut(s) 1534, 1557
Eco130I CCWWGG 2 cut(s) 449, 920
Eco24I GRGCYC 1 cut(s) 1615
Eco31I GGTCTC 2 cut(s) 56, 368
Eco32I GATATC 1 cut(s) 1376
Eco47I GGWCC 2 cut(s) 25, 801
Eco88I CYCGRG 1 cut(s) 713
Eco91I GGTNACC 1 cut(s) 1297
EcoO65I GGTNACC 1 cut(s) 1297
EcoRII CCWGG 2 cut(s) 720, 804
EcoRV GATATC 1 cut(s) 1376
EcoT14I CCWWGG 2 cut(s) 449, 920
EcoT38I GRGCYC 1 cut(s) 1615
ErhI CCWWGG 2 cut(s) 449, 920
FaeI CATG 5 cut(s) 251, 828, 844, 979, 1160
FalI AAGNNNNNCTT 2 cut(s) 1286, 1318
FaqI GGGAC 3 cut(s) 560, 787, 1450
FatI CATG 5 cut(s) 247, 824, 840, 975, 1156
FauNDI CATATG 2 cut(s) 875, 1605
FbaI TGATCA 1 cut(s) 837
Fnu4HI GCNGC 2 cut(s) 1205, 1576
FokI GGATG 8 cut(s) 553, 779, 1143, 1251, 1358, 1479, 1699, 1779
FriOI GRGCYC 1 cut(s) 1615
Fsp4HI GCNGC 2 cut(s) 1205, 1576
FspBI CTAG 8 cut(s) 122, 489, 708, 882, 957, 1170, 1352, 1542
GlaI GCGC 2 cut(s) 1019, 1755
GluI GCNGC 2 cut(s) 1205, 1576
HaeII RGCGCY 1 cut(s) 1021
HaeIII GGCC 2 cut(s) 272, 1478
HapII CCGG 1 cut(s) 23
HgaI GACGC 1 cut(s) 299
HhaI GCGC 2 cut(s) 1020, 1756
Hin1II CATG 5 cut(s) 251, 828, 844, 979, 1160
Hin6I GCGC 2 cut(s) 1018, 1754
HinP1I GCGC 2 cut(s) 1018, 1754
HinfI GANTC 3 cut(s) 785, 1247, 1736
HpaII CCGG 1 cut(s) 23
HphI GGTGA 6 cut(s) 466, 821, 937, 1309, 1644, 1693
Hpy166II GTNNAC 4 cut(s) 446, 812, 1000, 1385
Hpy188I TCNGA 3 cut(s) 293, 1052, 1150
Hpy8I GTNNAC 4 cut(s) 446, 812, 1000, 1385
Hpy99I CGWCG 2 cut(s) 315, 410
HpyCH4III ACNGT 2 cut(s) 1289, 1656
HpyCH4IV ACGT 2 cut(s) 522, 1002
HpyF10VI GCNNNNNNNGC 5 cut(s) 116, 1073, 1201, 1484, 1760
HpyF3I CTNAG 2 cut(s) 284, 1147
HpySE526I ACGT 2 cut(s) 522, 1002
Hsp92II CATG 5 cut(s) 251, 828, 844, 979, 1160
HspAI GCGC 2 cut(s) 1018, 1754
Ksp22I TGATCA 1 cut(s) 837
Kzo9I GATC 6 cut(s) 343, 456, 604, 837, 1052, 1279
LmnI GCTCC 1 cut(s) 294
Lsp1109I GCAGC 1 cut(s) 1562
LweI GCATC 3 cut(s) 97, 165, 1677
MaeI CTAG 8 cut(s) 122, 489, 708, 882, 957, 1170, 1352, 1542
MaeII ACGT 2 cut(s) 522, 1002
MaeIII GTNAC 3 cut(s) 657, 893, 1297
MalI GATC 6 cut(s) 345, 458, 606, 839, 1054, 1281
MbiI CCGCTC 1 cut(s) 1204
MboI GATC 6 cut(s) 343, 456, 604, 837, 1052, 1279
MflI RGATCY 2 cut(s) 1052, 1279
MhlI GDGCHC 3 cut(s) 641, 1103, 1615
MlsI TGGCCA 1 cut(s) 1478
MluNI TGGCCA 1 cut(s) 1478
MlyI GAGTC 1 cut(s) 1730
MmeI TCCRAC 1 cut(s) 321
Mox20I TGGCCA 1 cut(s) 1478
MscI TGGCCA 1 cut(s) 1478
MseI TTAA 5 cut(s) 63, 572, 791, 1461, 1825
MslI CAYNNNNRTG 3 cut(s) 71, 595, 1340
Msp20I TGGCCA 1 cut(s) 1478
MspI CCGG 1 cut(s) 23
MspR9I CCNGG 2 cut(s) 722, 806
Mva1269I GAATGC 1 cut(s) 674
MvaI CCWGG 2 cut(s) 722, 806
MvnI CGCG 1 cut(s) 310
MwoI GCNNNNNNNGC 5 cut(s) 116, 1073, 1201, 1484, 1760
NdeI CATATG 2 cut(s) 875, 1605
NdeII GATC 6 cut(s) 343, 456, 604, 837, 1052, 1279
NlaIII CATG 5 cut(s) 251, 828, 844, 979, 1160
NlaIV GGNNCC 2 cut(s) 290, 803
NmuCI GTSAC 3 cut(s) 657, 893, 1297
NspI RCATGY 1 cut(s) 828
OliI CACNNNNGTG 1 cut(s) 71
PaeR7I CTCGAG 1 cut(s) 713
PagI TCATGA 2 cut(s) 247, 840
PasI CCCWGGG 1 cut(s) 805
PciI ACATGT 1 cut(s) 824
PctI GAATGC 1 cut(s) 674
PfeI GAWTC 2 cut(s) 785, 1247
PkrI GCNGC 2 cut(s) 1206, 1577
PleI GAGTC 1 cut(s) 1730
PpsI GAGTC 1 cut(s) 1730
PscI ACATGT 1 cut(s) 824
Psp1406I AACGTT 2 cut(s) 522, 1002
Psp6I CCWGG 2 cut(s) 720, 804
PspEI GGTNACC 1 cut(s) 1297
PspGI CCWGG 2 cut(s) 720, 804
PspN4I GGNNCC 2 cut(s) 290, 803
PspPI GGNCC 2 cut(s) 25, 801
PstNI CAGNNNCTG 1 cut(s) 116
PsuI RGATCY 2 cut(s) 1052, 1279
RsaI GTAC 2 cut(s) 400, 1384
RsaNI GTAC 2 cut(s) 399, 1383
RseI CAYNNNNRTG 3 cut(s) 71, 595, 1340
SaqAI TTAA 5 cut(s) 63, 572, 791, 1461, 1825
SatI GCNGC 2 cut(s) 1205, 1576
Sau3AI GATC 6 cut(s) 343, 456, 604, 837, 1052, 1279
Sau96I GGNCC 2 cut(s) 25, 801
SchI GAGTC 1 cut(s) 1730
ScrFI CCNGG 2 cut(s) 722, 806
SduI GDGCHC 3 cut(s) 641, 1103, 1615
SfaNI GCATC 3 cut(s) 97, 165, 1677
Sfr274I CTCGAG 1 cut(s) 713
SinI GGWCC 2 cut(s) 25, 801
SlaI CTCGAG 1 cut(s) 713
SmiMI CAYNNNNRTG 3 cut(s) 71, 595, 1340
SmlI CTYRAG 4 cut(s) 365, 713, 746, 1175
SmoI CTYRAG 4 cut(s) 365, 713, 746, 1175
SpeI ACTAGT 2 cut(s) 881, 956
SsiI CCGC 1 cut(s) 1204
SspMI CTAG 8 cut(s) 122, 489, 708, 882, 957, 1170, 1352, 1542
StyD4I CCNGG 2 cut(s) 720, 804
StyI CCWWGG 2 cut(s) 449, 920
TaaI ACNGT 2 cut(s) 1289, 1656
TaiI ACGT 2 cut(s) 525, 1005
TaqII GACCGA 2 cut(s) 38, 1460
TatI WGTACW 1 cut(s) 398
TauI GCSGC 1 cut(s) 1207
TfiI GAWTC 2 cut(s) 785, 1247
Tru1I TTAA 5 cut(s) 63, 572, 791, 1461, 1825
Tru9I TTAA 5 cut(s) 63, 572, 791, 1461, 1825
TscAI CASTG 3 cut(s) 73, 666, 1103
TseFI GTSAC 3 cut(s) 657, 893, 1297
TseI GCWGC 1 cut(s) 1575
Tsp45I GTSAC 3 cut(s) 657, 893, 1297
TspDTI ATGAA 5 cut(s) 344, 857, 1245, 1253, 1656
TspGWI ACGGA 5 cut(s) 423, 437, 1036, 1386, 1785
TspRI CASTG 3 cut(s) 73, 666, 1103
VpaK11BI GGWCC 2 cut(s) 25, 801
XapI RAATTY 7 cut(s) 221, 929, 947, 1165, 1358, 1643, 1665
XceI RCATGY 1 cut(s) 828
XhoI CTCGAG 1 cut(s) 713
XspI CTAG 8 cut(s) 122, 489, 708, 882, 957, 1170, 1352, 1542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.