FvH4_5g01581

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001401
Physical Location & Seq
Forward (+)
971556 .. 972553
998 bp
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UTR
Exon/CDS
Intron
FvH4_5g01581.t1

Sequence Viewer

Length: 441 bp
ATGAACTATTTGAACTCACAGGTCATCAATGAAACGGTTAGACTGGCAAGTCAAGTGCCAGGCATCTTTCGCAAGGCAATAAAAGAGGTACAGTTCAAGGGATATACGATCCCAGAAGGTTGGTCAGTTCTGGTTTGTTTCCAACCCCAGCACTTAAACCCAGAAAAGTATGAAGATCCTCTGGCCTTCAACCCATGGAGATGGGAGGGTAAGGAAAGTGGTGCATCCAAAGATTTTATGGCTTTTGGTGGTGGCTTGAGATCCTGCATAGGAGCAGACTTTGGTAAGGTGAATATGGCTGTATTCGTCCATTTCTTAGTCACAAAGTACAGGTGGCAAGTGATCGGAGGCAAGATGGTACGAAATCCTACTATCCAATTTCCAGGTGGTTTTCACATACGAATGCAGCAGAAAGATACAAGGAAGGGCGAACAGCACTAA

Protein Analysis

147

Amino Acids

16.72

Weight (kDa)

9.61

Isoelectric Point (pI)

33.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 1 - 126 1e-30 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 48
AclWI GGATC 3 cut(s) 103, 170, 255
AfaI GTAC 3 cut(s) 90, 329, 360
AgsI TTSAA 3 cut(s) 13, 97, 190
AjnI CCWGG 2 cut(s) 58, 382
AlwI GGATC 3 cut(s) 103, 170, 255
AoxI GGCC 1 cut(s) 183
ApeKI GCWGC 1 cut(s) 406
AsuHPI GGTGA 1 cut(s) 301
BbvI GCAGC 1 cut(s) 418
BccI CCATC 2 cut(s) 195, 349
BciT130I CCWGG 2 cut(s) 60, 384
BisI GCNGC 1 cut(s) 407
BlsI GCNGC 1 cut(s) 408
Bme1390I CCNGG 2 cut(s) 60, 384
BmrFI CCNGG 2 cut(s) 60, 384
BmsI GCATC 2 cut(s) 72, 233
BpuEI CTTGAG 1 cut(s) 277
BsaJI CCNNGG 1 cut(s) 194
Bse1I ACTGG 1 cut(s) 48
BseBI CCWGG 2 cut(s) 60, 384
BseDI CCNNGG 1 cut(s) 194
BseGI GGATG 1 cut(s) 224
BseNI ACTGG 1 cut(s) 48
BseXI GCAGC 1 cut(s) 418
BseYI CCCAGC 1 cut(s) 147
BshFI GGCC 1 cut(s) 185
BsmI GAATGC 1 cut(s) 408
BsnI GGCC 1 cut(s) 185
Bsp143I GATC 4 cut(s) 108, 175, 260, 342
Bsp19I CCATGG 1 cut(s) 194
BspANI GGCC 1 cut(s) 185
BspPI GGATC 3 cut(s) 103, 170, 255
BsrI ACTGG 1 cut(s) 48
BssECI CCNNGG 1 cut(s) 194
BssMI GATC 4 cut(s) 108, 175, 260, 342
BssT1I CCWWGG 1 cut(s) 194
Bst2UI CCWGG 2 cut(s) 60, 384
Bst4CI ACNGT 2 cut(s) 37, 93
BstDEI CTNAG 1 cut(s) 316
BstDSI CCRYGG 1 cut(s) 194
BstF5I GGATG 1 cut(s) 224
BstKTI GATC 4 cut(s) 111, 178, 263, 345
BstMBI GATC 4 cut(s) 108, 175, 260, 342
BstMWI GCNNNNNNNGC 1 cut(s) 69
BstNI CCWGG 2 cut(s) 60, 384
BstSCI CCNGG 2 cut(s) 58, 382
BstV1I GCAGC 1 cut(s) 418
BstX2I RGATCY 2 cut(s) 175, 260
BstXI CCANNNNNNTGG 2 cut(s) 120, 201
BstYI RGATCY 2 cut(s) 175, 260
BsuRI GGCC 1 cut(s) 185
BtgI CCRYGG 1 cut(s) 194
BtsCI GGATG 1 cut(s) 224
Csp6I GTAC 3 cut(s) 89, 328, 359
CviAII CATG 1 cut(s) 195
CviJI RGCY 4 cut(s) 185, 242, 255, 299
CviKI_1 RGCY 4 cut(s) 185, 242, 255, 299
CviQI GTAC 3 cut(s) 89, 328, 359
DdeI CTNAG 1 cut(s) 316
DpnI GATC 4 cut(s) 110, 177, 262, 344
DpnII GATC 4 cut(s) 108, 175, 260, 342
DrdI GACNNNNNNGTC 1 cut(s) 48
DseDI GACNNNNNNGTC 1 cut(s) 48
Eco130I CCWWGG 1 cut(s) 194
EcoRII CCWGG 2 cut(s) 58, 382
EcoT14I CCWWGG 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 194
FaeI CATG 1 cut(s) 198
FaiI YATR 7 cut(s) 105, 171, 196, 239, 269, 296, 398
FatI CATG 1 cut(s) 194
Fnu4HI GCNGC 1 cut(s) 407
FokI GGATG 1 cut(s) 211
Fsp4HI GCNGC 1 cut(s) 407
GluI GCNGC 1 cut(s) 407
GsaI CCCAGC 1 cut(s) 151
HaeIII GGCC 1 cut(s) 185
Hin1II CATG 1 cut(s) 198
HphI GGTGA 1 cut(s) 301
Hpy188I TCNGA 1 cut(s) 347
HpyAV CCTTC 3 cut(s) 110, 196, 418
HpyCH4III ACNGT 2 cut(s) 37, 93
HpyCH4V TGCA 3 cut(s) 224, 267, 406
HpyF10VI GCNNNNNNNGC 1 cut(s) 69
HpyF3I CTNAG 1 cut(s) 316
Hsp92II CATG 1 cut(s) 198
Kzo9I GATC 4 cut(s) 108, 175, 260, 342
LmnI GCTCC 1 cut(s) 272
Lsp1109I GCAGC 1 cut(s) 418
LweI GCATC 2 cut(s) 72, 233
MaeIII GTNAC 1 cut(s) 319
MalI GATC 4 cut(s) 110, 177, 262, 344
MboI GATC 4 cut(s) 108, 175, 260, 342
MboII GAAGA 1 cut(s) 185
MflI RGATCY 2 cut(s) 175, 260
MluCI AATT 1 cut(s) 377
MmeI TCCRAC 1 cut(s) 166
MnlI CCTC 4 cut(s) 79, 189, 199, 341
MseI TTAA 1 cut(s) 155
MslI CAYNNNNRTG 2 cut(s) 199, 401
MspR9I CCNGG 2 cut(s) 60, 384
Mva1269I GAATGC 1 cut(s) 408
MvaI CCWGG 2 cut(s) 60, 384
MwoI GCNNNNNNNGC 1 cut(s) 69
NcoI CCATGG 1 cut(s) 194
NdeII GATC 4 cut(s) 108, 175, 260, 342
NlaIII CATG 1 cut(s) 198
NmuCI GTSAC 1 cut(s) 319
PctI GAATGC 1 cut(s) 408
PkrI GCNGC 1 cut(s) 408
Psp6I CCWGG 2 cut(s) 58, 382
PspFI CCCAGC 1 cut(s) 147
PspGI CCWGG 2 cut(s) 58, 382
PsuI RGATCY 2 cut(s) 175, 260
RsaI GTAC 3 cut(s) 90, 329, 360
RsaNI GTAC 3 cut(s) 89, 328, 359
RseI CAYNNNNRTG 2 cut(s) 199, 401
SaqAI TTAA 1 cut(s) 155
SatI GCNGC 1 cut(s) 407
Sau3AI GATC 4 cut(s) 108, 175, 260, 342
ScrFI CCNGG 2 cut(s) 60, 384
SetI ASST 6 cut(s) 24, 90, 121, 291, 335, 388
SfaNI GCATC 2 cut(s) 72, 233
SmiMI CAYNNNNRTG 2 cut(s) 199, 401
SmlI CTYRAG 1 cut(s) 256
SmoI CTYRAG 1 cut(s) 256
Sse9I AATT 1 cut(s) 377
StyD4I CCNGG 2 cut(s) 58, 382
StyI CCWWGG 1 cut(s) 194
TaaI ACNGT 2 cut(s) 37, 93
TasI AATT 1 cut(s) 377
TatI WGTACW 1 cut(s) 327
Tru1I TTAA 1 cut(s) 155
Tru9I TTAA 1 cut(s) 155
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 1 cut(s) 406
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 3 cut(s) 17, 45, 186
XcmI CCANNNNNNNNNTGG 2 cut(s) 235, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.