Rh4BG380800

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001401
Physical Location & Seq
Reverse (-)
53619414 .. 53624093
4680 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG380800.1

Sequence Viewer

Length: 789 bp
ATGTTTGTACTGCTTTTCGCGAGCTTTGAAACAACTTCCCTAGCTCTAACATTAGCCATCAAGTTTCTTTCAGACCATCCTGCAGTGCTAAAGAAATTAACGGAAGAGCATGGGATGATCATAAAGCAAAGGGAAAATGCTGATGCTGGTCTTACATGGAAAGAATACAAATCAATGACATTCACATTTCAGATCATCAACGAAACAGTTAGACTGGCAAATATAGTTCCAGCTATCTTTCGCAAAGCAATAAGAGAGATACAGTTTAAAGGACATACCATTCCAGCAGGTTGGGCAGTAATGGTTTGTCCCCCTGCCGTACACTTAAACCCTCAAAAATATGATGATCCTGTGGCCTTCAATCCAGGGAGATGGGAGGGAATGTTAACAAGTAGTGCATCAAAAAATTTCATGGCTTTTGGCGGTGGCATGAGATTCTGTGTTGGAACAGACTTTGCTAAGGTGCAGATGGCTGTATTTCTGCATTGCTTGGTGACGAAGTACAGGTGGCAAGCAGTGGGGGGAGGAAATATAGTCCGAACTCCGGGTTTGAACTATGAAAAGGAGTGTAGAAGGAGCAATGACAACTTGTATACCAGCTGGAACTGGATTGGTGGATCTGGTATGGATCTCAGAAGCAAAGCAAGGACTCTGCCAGGACCTGTTAGGAGAGGCAACAATATCTTGGTACTGGATGCAAAGCCTTTTTTGAAGGAGGCCCTTCAAGCCGAAGCACGGGGTGGGGTTGAGGTCGGGAAGGAAGATATGGGATACTGGAGGAAGGATTAA

Protein Analysis

262

Amino Acids

29.39

Weight (kDa)

9.51

Isoelectric Point (pI)

38.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 2 - 176 3.1e-45 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 278
AccI GTMKAC 1 cut(s) 593
AccII CGCG 1 cut(s) 20
AciI CCGC 1 cut(s) 423
AclWI GGATC 3 cut(s) 341, 625, 636
AcsI RAATTY 1 cut(s) 406
AdeI CACNNNGTG 1 cut(s) 740
AfaI GTAC 4 cut(s) 9, 321, 503, 690
AfiI CCNNNNNNNGG 2 cut(s) 544, 735
AgsI TTSAA 5 cut(s) 29, 361, 553, 712, 725
AjnI CCWGG 2 cut(s) 364, 655
AluBI AGCT 4 cut(s) 24, 44, 233, 600
AluI AGCT 4 cut(s) 24, 44, 233, 600
AlwI GGATC 3 cut(s) 341, 625, 636
AlwNI CAGNNNCTG 1 cut(s) 662
AoxI GGCC 2 cut(s) 354, 717
ApoI RAATTY 1 cut(s) 406
AspS9I GGNCC 2 cut(s) 659, 718
AsuC2I CCSGG 1 cut(s) 546
AsuHPI GGTGA 1 cut(s) 505
AvaII GGWCC 1 cut(s) 659
BccI CCATC 4 cut(s) 65, 84, 366, 463
BceAI ACGGC 1 cut(s) 302
BciT130I CCWGG 2 cut(s) 366, 657
BciVI GTATCC 1 cut(s) 764
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 1 cut(s) 546
BfaI CTAG 1 cut(s) 41
BfmI CTRYAG 1 cut(s) 81
BfuAI ACCTGC 1 cut(s) 278
BfuI GTATCC 1 cut(s) 764
Bme1390I CCNGG 3 cut(s) 366, 546, 657
Bme18I GGWCC 1 cut(s) 659
BmgT120I GGNCC 2 cut(s) 659, 718
BmrFI CCNGG 3 cut(s) 366, 546, 657
BmsI GCATC 3 cut(s) 133, 407, 685
Bpu10I CCTNAGC 1 cut(s) 459
BpuMI CCSGG 1 cut(s) 546
BsaJI CCNNGG 1 cut(s) 365
BsaXI ACNNNNNCTCC 2 cut(s) 368, 398
Bsc4I CCNNNNNNNGG 2 cut(s) 544, 735
Bse1I ACTGG 4 cut(s) 219, 611, 696, 779
Bse3DI GCAATG 2 cut(s) 484, 586
BseBI CCWGG 2 cut(s) 366, 657
BseDI CCNNGG 1 cut(s) 365
BseGI GGATG 3 cut(s) 76, 120, 700
BseLI CCNNNNNNNGG 2 cut(s) 544, 735
BseMI GCAATG 2 cut(s) 484, 586
BseMII CTCAG 1 cut(s) 646
BseNI ACTGG 4 cut(s) 219, 611, 696, 779
BsgI GTGCAG 1 cut(s) 485
Bsh1236I CGCG 1 cut(s) 20
BshFI GGCC 2 cut(s) 356, 719
BsiSI CCGG 1 cut(s) 545
BslFI GGGAC 1 cut(s) 294
BslI CCNNNNNNNGG 2 cut(s) 544, 735
BsmFI GGGAC 1 cut(s) 294
BsnI GGCC 2 cut(s) 356, 719
Bsp143I GATC 5 cut(s) 117, 192, 346, 617, 628
Bsp68I TCGCGA 1 cut(s) 20
BspACI CCGC 1 cut(s) 423
BspANI GGCC 2 cut(s) 356, 719
BspCNI CTCAG 1 cut(s) 645
BspFNI CGCG 1 cut(s) 20
BspMAI CTGCAG 1 cut(s) 85
BspMI ACCTGC 1 cut(s) 278
BspPI GGATC 3 cut(s) 341, 625, 636
BspQI GCTCTTC 1 cut(s) 99
BsrDI GCAATG 2 cut(s) 484, 586
BsrI ACTGG 4 cut(s) 219, 611, 696, 779
BssECI CCNNGG 1 cut(s) 365
BssMI GATC 5 cut(s) 117, 192, 346, 617, 628
BssNAI GTATAC 1 cut(s) 594
Bst1107I GTATAC 1 cut(s) 594
Bst2UI CCWGG 2 cut(s) 366, 657
Bst4CI ACNGT 2 cut(s) 208, 264
Bst6I CTCTTC 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 22, 513
BstDEI CTNAG 2 cut(s) 459, 632
BstF5I GGATG 3 cut(s) 76, 120, 700
BstFNI CGCG 1 cut(s) 20
BstKTI GATC 5 cut(s) 120, 195, 349, 620, 631
BstMBI GATC 5 cut(s) 117, 192, 346, 617, 628
BstMWI GCNNNNNNNGC 2 cut(s) 293, 725
BstNI CCWGG 2 cut(s) 366, 657
BstSCI CCNGG 3 cut(s) 364, 544, 655
BstSFI CTRYAG 1 cut(s) 81
BstUI CGCG 1 cut(s) 20
BstX2I RGATCY 2 cut(s) 617, 628
BstXI CCANNNNNNTGG 2 cut(s) 291, 372
BstYI RGATCY 2 cut(s) 617, 628
BstZ17I GTATAC 1 cut(s) 594
BsuI GTATCC 1 cut(s) 764
BsuRI GGCC 2 cut(s) 356, 719
BtsCI GGATG 3 cut(s) 76, 120, 700
BtsI GCAGTG 2 cut(s) 90, 522
BtsIMutI CAGTG 2 cut(s) 90, 522
BtuMI TCGCGA 1 cut(s) 20
BveI ACCTGC 1 cut(s) 278
Cac8I GCNNGC 2 cut(s) 22, 513
CaiI CAGNNNCTG 1 cut(s) 662
Cfr13I GGNCC 2 cut(s) 659, 718
Csp6I GTAC 4 cut(s) 8, 320, 502, 689
CviAII CATG 4 cut(s) 110, 156, 412, 430
CviQI GTAC 4 cut(s) 8, 320, 502, 689
DdeI CTNAG 2 cut(s) 459, 632
DpnI GATC 5 cut(s) 119, 194, 348, 619, 630
DpnII GATC 5 cut(s) 117, 192, 346, 617, 628
DraI TTTAAA 1 cut(s) 268
DraIII CACNNNGTG 1 cut(s) 740
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Eco47I GGWCC 1 cut(s) 659
EcoO109I RGGNCCY 2 cut(s) 659, 718
EcoRII CCWGG 2 cut(s) 364, 655
FaeI CATG 4 cut(s) 113, 159, 415, 433
FaqI GGGAC 1 cut(s) 294
FatI CATG 4 cut(s) 109, 155, 411, 429
FbaI TGATCA 1 cut(s) 117
FblI GTMKAC 1 cut(s) 593
FokI GGATG 3 cut(s) 63, 127, 707
FspBI CTAG 1 cut(s) 41
HaeIII GGCC 2 cut(s) 356, 719
HapII CCGG 1 cut(s) 545
Hin1II CATG 4 cut(s) 113, 159, 415, 433
HincII GTYRAC 1 cut(s) 387
HindII GTYRAC 1 cut(s) 387
HinfI GANTC 2 cut(s) 435, 649
HpaI GTTAAC 1 cut(s) 387
HpaII CCGG 1 cut(s) 545
HphI GGTGA 1 cut(s) 505
Hpy166II GTNNAC 3 cut(s) 322, 387, 594
Hpy188I TCNGA 4 cut(s) 73, 192, 539, 635
Hpy188III TCNNGA 2 cut(s) 19, 754
Hpy8I GTNNAC 3 cut(s) 322, 387, 594
HpyAV CCTTC 6 cut(s) 367, 567, 706, 731, 751, 775
HpyCH4III ACNGT 2 cut(s) 208, 264
HpyCH4V TGCA 5 cut(s) 83, 398, 466, 484, 698
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 725
HpyF3I CTNAG 2 cut(s) 459, 632
Hsp92II CATG 4 cut(s) 113, 159, 415, 433
Ksp22I TGATCA 1 cut(s) 117
KspAI GTTAAC 1 cut(s) 387
Kzo9I GATC 5 cut(s) 117, 192, 346, 617, 628
LguI GCTCTTC 1 cut(s) 99
LmnI GCTCC 1 cut(s) 576
LweI GCATC 3 cut(s) 133, 407, 685
MaeI CTAG 1 cut(s) 41
MaeIII GTNAC 1 cut(s) 493
MalI GATC 5 cut(s) 119, 194, 348, 619, 630
MboI GATC 5 cut(s) 117, 192, 346, 617, 628
MboII GAAGA 2 cut(s) 116, 773
MflI RGATCY 2 cut(s) 617, 628
MluCI AATT 2 cut(s) 95, 406
MlyI GAGTC 1 cut(s) 643
MmeI TCCRAC 1 cut(s) 424
MnlI CCTC 7 cut(s) 342, 370, 518, 665, 709, 742, 771
MseI TTAA 5 cut(s) 98, 267, 326, 386, 787
MspA1I CMGCKG 1 cut(s) 600
MspI CCGG 1 cut(s) 545
MspR9I CCNGG 3 cut(s) 366, 546, 657
MvaI CCWGG 2 cut(s) 366, 657
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 2 cut(s) 293, 725
NciI CCSGG 1 cut(s) 546
NdeII GATC 5 cut(s) 117, 192, 346, 617, 628
NlaIII CATG 4 cut(s) 113, 159, 415, 433
NmuCI GTSAC 1 cut(s) 493
NruI TCGCGA 1 cut(s) 20
PciSI GCTCTTC 1 cut(s) 99
PfeI GAWTC 1 cut(s) 435
PleI GAGTC 1 cut(s) 643
PpsI GAGTC 1 cut(s) 643
PpuMI RGGWCCY 1 cut(s) 659
Psp5II RGGWCCY 1 cut(s) 659
Psp6I CCWGG 2 cut(s) 364, 655
PspGI CCWGG 2 cut(s) 364, 655
PspPI GGNCC 2 cut(s) 659, 718
PspPPI RGGWCCY 1 cut(s) 659
PstI CTGCAG 1 cut(s) 85
PstNI CAGNNNCTG 1 cut(s) 662
PsuI RGATCY 2 cut(s) 617, 628
PvuII CAGCTG 1 cut(s) 600
RruI TCGCGA 1 cut(s) 20
RsaI GTAC 4 cut(s) 9, 321, 503, 690
RsaNI GTAC 4 cut(s) 8, 320, 502, 689
SapI GCTCTTC 1 cut(s) 99
SaqAI TTAA 5 cut(s) 98, 267, 326, 386, 787
Sau3AI GATC 5 cut(s) 117, 192, 346, 617, 628
Sau96I GGNCC 2 cut(s) 659, 718
SchI GAGTC 1 cut(s) 643
ScrFI CCNGG 3 cut(s) 366, 546, 657
SetI ASST 9 cut(s) 26, 46, 235, 292, 465, 509, 602, 664, 753
SfaNI GCATC 3 cut(s) 133, 407, 685
SfcI CTRYAG 1 cut(s) 81
SinI GGWCC 1 cut(s) 659
Sse9I AATT 2 cut(s) 95, 406
SsiI CCGC 1 cut(s) 423
SspMI CTAG 1 cut(s) 41
StyD4I CCNGG 3 cut(s) 364, 544, 655
TaaI ACNGT 2 cut(s) 208, 264
TasI AATT 2 cut(s) 95, 406
TatI WGTACW 2 cut(s) 7, 501
TfiI GAWTC 1 cut(s) 435
Tru1I TTAA 5 cut(s) 98, 267, 326, 386, 787
Tru9I TTAA 5 cut(s) 98, 267, 326, 386, 787
TscAI CASTG 2 cut(s) 90, 522
TseFI GTSAC 1 cut(s) 493
Tsp45I GTSAC 1 cut(s) 493
TspDTI ATGAA 2 cut(s) 400, 573
TspGWI ACGGA 1 cut(s) 116
TspRI CASTG 2 cut(s) 90, 522
VpaK11BI GGWCC 1 cut(s) 659
XapI RAATTY 1 cut(s) 406
XmiI GTMKAC 1 cut(s) 593
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.