Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0002154

Physical Location & Seq

Genomic Coordinates Reverse (-)

11577663 .. 11579162

Gene Structure 1500 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g19750.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1179 bp

ATGGGTAGCATAGCAACAAGGTGGAAGGCTCTGAGCGGCCAGGACGAGTGGGAGGGTCTATTAAACCCTCTCGACATCGATCTCCGCCGCTATATCATTCACTACGGGGAAAGGGCTGGAGCGATAGCCGTGGTGAACGAGCCAAAATCGAAGAACTACAGACTTCCACGATACGCGAAGGAAAATTTGTTCTCAAATATGGGATTGGAGAGTGGCAATCCATACAAGTACATGGTGAATAAATATTTGTATGCAGCCCATATGACATTTTCGTTACAGAAATCAAACTTTCTGGGTTATGTTGCGGTGACAACAGATGAAGGAGCCAAAGTGTTGGGGAGGAGGGATATTTTGATTTCTTGGAGAGGAACCCAATTGGTACATGAGTGGGATGTCGACAAGGAGACCGATTTAGTCTCAGCTTCTCACATTCTGGGAGAGGAGAATGATCCTCTGGTGCACCAGGGTTGGCTTTCCTATTACACTAATATTGACCTTCTGTCCCCGCACAATCAACTTTCTAGTTGCAGAGATCAGGCTGTAGCTGCACTTAAAGAAATACTAGATGAATACAAGGATGAAGAAATCAGCATTACCATCACTGGTCACAGCATGGGTGCTGCCATGGCAATTTTAAACGCAGCCGATATTGTTCATAACGGATACAATAAACTGGCATGTCAACCAAACAAGCTCATTCCTGTCACAGCCATTGTGTTTGCTTGCCCCCGTCTCGGAAACAAGGGTTTCGAAAATGTATTTTCCGGTCTCGAAAATCTTCACGTCTTGCGTATAACAAATGACCAAGATATAGTCCCTAACTTACCAGATCTGGAGGATTATGCTCATGTTGGAAAAGAGTTGAGAATCGACACTCTCAAGTCGCCGTTCTTGAAAGTAATTCCTGATACTCTTGTCACAGAAAGAATTGGTAATTTGCACAACCTGGAGGTTTACTTGCATGGAGTTGCAGGGACACAAGGAGTCAAAAGTTCTGGTTTCAAGATGGAAGTTGATCGTGATCTCGCGCTGGTAAACAAGCATATTGATGGGTTGAAGGATGAACATAATGTAGTTGCTCAGTGGTGGACGGAGAAGAATAAGTCTATGATCCAAAAGGATGATGGATCTTGGGTGTTGGATGATCACGAGGAAGATGCGACTATATGTTTAGTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006638 GO:0006639 GO:0008150 GO:0008152 GO:0008970 GO:0009056 GO:0009987 GO:0010029 GO:0010187 GO:0010876 GO:0016042 GO:0016298 GO:0016787 GO:0016788 GO:0019915 GO:0033036 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044424 GO:0044464 GO:0046339 GO:0046340 GO:0046461 GO:0046462 GO:0046464 GO:0046486 GO:0046503 GO:0047372 GO:0048519 GO:0048580 GO:0048581 GO:0050789 GO:0050793 GO:0051093 GO:0051179 GO:0051235 GO:0051239 GO:0051241 GO:0052651 GO:0052689 GO:0065007 GO:0065008 GO:0071704 GO:1900140 GO:1901575 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lipase_3

Protein Analysis

393

Amino Acids

44.07

Weight (kDa)

5.38

Isoelectric Point (pI)

36.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipase_3	PF01764	119 - 277	2.9e-31	Lipase (class 3)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002154)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g19750 FvH4_5g19760
malus_domestica	MD04G1058700.v1.1
prunus_persica	Prupe.5G062500_v2.0.a1 Prupe.5G062600_v2.0.a1 Prupe.5G062700_v2.0.a1
pyrus_communis	pycom04g05260
rosa_chinensis	RchiOBHm_Chr7g0203651 RchiOBHm_Chr7g0203681 RchiOBHm_Chr7g0204521 RchiOBHm_Chr7g0204541
rosa_laevigata	RLG00000003452 RLG00000003453 RLG00000003512
rosa_multiflora	Rmu_co8274427.1_g000001 Rmu_co8340383.1_g000001 Rmu_sc0005354.1_g000007 Rmu_ssc0000443.1_g000023 Rmu_ssc0000443.1_g000033
rosa_roxburghii	Rroxscaffold_3G00253010 Rroxscaffold_3G00253020 Rroxscaffold_3G00253540 Rroxscaffold_3G00253610 Rroxscaffold_6G00397050 Rroxscaffold_6G00397100
rosa_rugosa	Rorug07G0078000 Rorug07G0084900 Rorug07G0085000
rosa_samantha	Rh7AG207600 Rh7AG214200 Rh7AG214300 Rh7BG206900 Rh7BG211700 Rh7BG211800 Rh7CG219900 Rh7CG227300 Rh7DG214500 Rh7DG214700 Rh7DG221800 Rh7DG222000
rosa_wichuraiana	Rw7G018100 Rw7G018570 Rw7G018580 Rw7G018590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	36
AccI	GTMKAC	1 cut(s)	396
AccII	CGCG	2 cut(s)	176, 1028
AciI	CCGC	5 cut(s)	36, 85, 88, 305, 506
AclWI	GGATC	3 cut(s)	443, 1105, 1135
AcoI	YGGCCR	1 cut(s)	37
AcsI	RAATTY	1 cut(s)	184
AfaI	GTAC	2 cut(s)	230, 381
AfiI	CCNNNNNNNGG	1 cut(s)	734
AgsI	TTSAA	3 cut(s)	895, 1003, 1057
AhdI	GACNNNNNGTC	1 cut(s)	499
AjiI	CACGTC	1 cut(s)	784
AjnI	CCWGG	3 cut(s)	39, 462, 945
AloI	GAACNNNNNNTCC	2 cut(s)	173, 205
AluBI	AGCT	3 cut(s)	422, 545, 694
AluI	AGCT	3 cut(s)	422, 545, 694
Alw21I	GWGCWC	1 cut(s)	462
Alw26I	GTCTC	4 cut(s)	398, 421, 737, 773
Alw44I	GTGCAC	1 cut(s)	458
AlwI	GGATC	3 cut(s)	443, 1105, 1135
AoxI	GGCC	1 cut(s)	37
ApaLI	GTGCAC	1 cut(s)	458
ApeKI	GCWGC	4 cut(s)	254, 545, 620, 641
ApoI	RAATTY	1 cut(s)	184
Asp700I	GAANNNNTTC	1 cut(s)	777
AspLEI	GCGC	1 cut(s)	1030
AsuHPI	GGTGA	3 cut(s)	145, 247, 319
AsuII	TTCGAA	1 cut(s)	750
BaeGI	GKGCMC	1 cut(s)	462
BaeI	ACNNNNGTAYC	4 cut(s)	371, 404, 900, 933
BauI	CACGAG	1 cut(s)	1148
Bbv12I	GWGCWC	1 cut(s)	462
BbvI	GCAGC	4 cut(s)	266, 532, 607, 653
BccI	CCATC	4 cut(s)	605, 1000, 1043, 1118
BceAI	ACGGC	2 cut(s)	113, 871
BcgI	CGANNNNNNTGC	2 cut(s)	1139, 1173
BciT130I	CCWGG	3 cut(s)	41, 464, 947
BciVI	GTATCC	1 cut(s)	656
BclI	TGATCA	1 cut(s)	1144
BcoDI	GTCTC	4 cut(s)	398, 421, 737, 773
BfaI	CTAG	3 cut(s)	522, 563, 1177
BfmI	CTRYAG	2 cut(s)	157, 540
BfuI	GTATCC	1 cut(s)	656
BglII	AGATCT	1 cut(s)	829
BisI	GCNGC	6 cut(s)	37, 88, 255, 546, 621, 642
BlsI	GCNGC	6 cut(s)	38, 89, 256, 547, 622, 643
Bme1390I	CCNGG	3 cut(s)	41, 464, 947
BmeRI	GACNNNNNGTC	1 cut(s)	499
BmgBI	CACGTC	1 cut(s)	784
BmiI	GGNNCC	2 cut(s)	325, 370
BmrFI	CCNGG	3 cut(s)	41, 464, 947
BmsI	GCATC	1 cut(s)	1147
BpmI	CTGGAG	3 cut(s)	138, 854, 968
Bpu14I	TTCGAA	1 cut(s)	750
BpuEI	CTTGAG	1 cut(s)	863
Bsa29I	ATCGAT	1 cut(s)	78
BsaBI	GATNNNNATC	1 cut(s)	1020
BsaI	GGTCTC	2 cut(s)	398, 773
BsaJI	CCNNGG	3 cut(s)	129, 463, 624
BsaWI	WCCGGW	1 cut(s)	764
BsaXI	ACNNNNNCTCC	2 cut(s)	315, 345
Bsc4I	CCNNNNNNNGG	1 cut(s)	734
Bse1I	ACTGG	2 cut(s)	607, 678
Bse8I	GATNNNNATC	1 cut(s)	1020
BseBI	CCWGG	3 cut(s)	41, 464, 947
BseCI	ATCGAT	1 cut(s)	78
BseDI	CCNNGG	3 cut(s)	129, 463, 624
BseGI	GGATG	5 cut(s)	397, 583, 1066, 1126, 1147
BseJI	GATNNNNATC	1 cut(s)	1020
BseLI	CCNNNNNNNGG	1 cut(s)	734
BseMII	CTCAG	3 cut(s)	23, 432, 1094
BseNI	ACTGG	2 cut(s)	607, 678
BseRI	GAGGAG	2 cut(s)	355, 455
BseSI	GKGCMC	1 cut(s)	462
BseXI	GCAGC	4 cut(s)	266, 532, 607, 653
BsgI	GTGCAG	1 cut(s)	531
Bsh1236I	CGCG	2 cut(s)	176, 1028
BshFI	GGCC	1 cut(s)	39
BshVI	ATCGAT	1 cut(s)	78
BsiHKAI	GWGCWC	1 cut(s)	462
BsiSI	CCGG	1 cut(s)	765
BslFI	GGGAC	3 cut(s)	487, 800, 988
BslI	CCNNNNNNNGG	1 cut(s)	734
BsmAI	GTCTC	4 cut(s)	398, 421, 737, 773
BsmBI	CGTCTC	1 cut(s)	737
BsmFI	GGGAC	3 cut(s)	487, 800, 988
BsnI	GGCC	1 cut(s)	39
Bso31I	GGTCTC	2 cut(s)	398, 773
Bsp119I	TTCGAA	1 cut(s)	750
Bsp1286I	GDGCHC	1 cut(s)	462
Bsp143I	GATC	9 cut(s)	79, 448, 532, 829, 1015, 1021, 1110, 1127, 1144
Bsp19I	CCATGG	1 cut(s)	624
BspACI	CCGC	5 cut(s)	36, 85, 88, 305, 506
BspANI	GGCC	1 cut(s)	39
BspCNI	CTCAG	3 cut(s)	24, 431, 1093
BspDI	ATCGAT	1 cut(s)	78
BspFNI	CGCG	2 cut(s)	176, 1028
BspLI	GGNNCC	2 cut(s)	325, 370
BspPI	GGATC	3 cut(s)	443, 1105, 1135
BspT104I	TTCGAA	1 cut(s)	750
BspTNI	GGTCTC	2 cut(s)	398, 773
BsrBI	CCGCTC	1 cut(s)	36
BsrI	ACTGG	2 cut(s)	607, 678
BssECI	CCNNGG	3 cut(s)	129, 463, 624
BssMI	GATC	9 cut(s)	79, 448, 532, 829, 1015, 1021, 1110, 1127, 1144
BssSI	CACGAG	1 cut(s)	1148
BssT1I	CCWWGG	1 cut(s)	624
Bst2BI	CACGAG	1 cut(s)	1148
Bst2UI	CCWGG	3 cut(s)	41, 464, 947
BstBI	TTCGAA	1 cut(s)	750
BstC8I	GCNNGC	1 cut(s)	724
BstDEI	CTNAG	3 cut(s)	32, 418, 1080
BstDSI	CCRYGG	2 cut(s)	129, 624
BstF5I	GGATG	5 cut(s)	397, 583, 1066, 1126, 1147
BstFNI	CGCG	2 cut(s)	176, 1028
BstHHI	GCGC	1 cut(s)	1030
BstKTI	GATC	9 cut(s)	82, 451, 535, 832, 1018, 1024, 1113, 1130, 1147
BstMAI	GTCTC	4 cut(s)	398, 421, 737, 773
BstMBI	GATC	9 cut(s)	79, 448, 532, 829, 1015, 1021, 1110, 1127, 1144
BstMWI	GCNNNNNNNGC	2 cut(s)	545, 626
BstNI	CCWGG	3 cut(s)	41, 464, 947
BstNSI	RCATGY	1 cut(s)	681
BstSCI	CCNGG	3 cut(s)	39, 462, 945
BstSFI	CTRYAG	2 cut(s)	157, 540
BstSLI	GKGCMC	1 cut(s)	462
BstUI	CGCG	2 cut(s)	176, 1028
BstV1I	GCAGC	4 cut(s)	266, 532, 607, 653
BstX2I	RGATCY	2 cut(s)	829, 1127
BstXI	CCANNNNNNTGG	1 cut(s)	334
BstYI	RGATCY	2 cut(s)	829, 1127
Bsu15I	ATCGAT	1 cut(s)	78
BsuI	GTATCC	1 cut(s)	656
BsuRI	GGCC	1 cut(s)	39
BsuTUI	ATCGAT	1 cut(s)	78
BtgI	CCRYGG	2 cut(s)	129, 624
BtrI	CACGTC	1 cut(s)	784
BtsCI	GGATG	5 cut(s)	397, 583, 1066, 1126, 1147
BtsIMutI	CAGTG	2 cut(s)	600, 1088
Cac8I	GCNNGC	1 cut(s)	724
CfoI	GCGC	1 cut(s)	1030
ClaI	ATCGAT	1 cut(s)	78
Csp6I	GTAC	2 cut(s)	229, 380
CviAII	CATG	7 cut(s)	232, 383, 613, 625, 678, 848, 962
CviQI	GTAC	2 cut(s)	229, 380
DdeI	CTNAG	3 cut(s)	32, 418, 1080
DpnI	GATC	9 cut(s)	81, 450, 534, 831, 1017, 1023, 1112, 1129, 1146
DpnII	GATC	9 cut(s)	79, 448, 532, 829, 1015, 1021, 1110, 1127, 1144
DraI	TTTAAA	1 cut(s)	636
DriI	GACNNNNNGTC	1 cut(s)	499
EaeI	YGGCCR	1 cut(s)	37
Eam1105I	GACNNNNNGTC	1 cut(s)	499
EciI	GGCGGA	1 cut(s)	74
Eco130I	CCWWGG	1 cut(s)	624
Eco31I	GGTCTC	2 cut(s)	398, 773
EcoRII	CCWGG	3 cut(s)	39, 462, 945
EcoT14I	CCWWGG	1 cut(s)	624
ErhI	CCWWGG	1 cut(s)	624
Esp3I	CGTCTC	1 cut(s)	737
FaeI	CATG	7 cut(s)	235, 386, 616, 628, 681, 851, 965
FaqI	GGGAC	3 cut(s)	487, 800, 988
FatI	CATG	7 cut(s)	231, 382, 612, 624, 677, 847, 961
FauI	CCCGC	1 cut(s)	513
FauNDI	CATATG	1 cut(s)	261
FbaI	TGATCA	1 cut(s)	1144
FblI	GTMKAC	1 cut(s)	396
Fnu4HI	GCNGC	6 cut(s)	37, 88, 255, 546, 621, 642
FokI	GGATG	5 cut(s)	404, 590, 1073, 1133, 1154
Fsp4HI	GCNGC	6 cut(s)	37, 88, 255, 546, 621, 642
FspBI	CTAG	3 cut(s)	522, 563, 1177
GlaI	GCGC	1 cut(s)	1029
GluI	GCNGC	6 cut(s)	37, 88, 255, 546, 621, 642
GsuI	CTGGAG	3 cut(s)	138, 854, 968
HaeIII	GGCC	1 cut(s)	39
HapII	CCGG	1 cut(s)	765
HhaI	GCGC	1 cut(s)	1030
Hin1II	CATG	7 cut(s)	235, 386, 616, 628, 681, 851, 965
Hin6I	GCGC	1 cut(s)	1028
HinP1I	GCGC	1 cut(s)	1028
HincII	GTYRAC	2 cut(s)	397, 683
HindII	GTYRAC	2 cut(s)	397, 683
HinfI	GANTC	2 cut(s)	867, 984
HpaII	CCGG	1 cut(s)	765
HphI	GGTGA	3 cut(s)	145, 247, 319
Hpy166II	GTNNAC	7 cut(s)	136, 397, 460, 683, 955, 1036, 1089
Hpy188I	TCNGA	2 cut(s)	33, 737
Hpy188III	TCNNGA	8 cut(s)	71, 770, 833, 892, 905, 1003, 1019, 1148
Hpy8I	GTNNAC	7 cut(s)	136, 397, 460, 683, 955, 1036, 1089
HpyAV	CCTTC	5 cut(s)	19, 172, 314, 506, 1051
HpyCH4IV	ACGT	1 cut(s)	783
HpyCH4V	TGCA	7 cut(s)	254, 460, 528, 548, 940, 961, 971
HpyF10VI	GCNNNNNNNGC	2 cut(s)	545, 626
HpyF3I	CTNAG	3 cut(s)	32, 418, 1080
HpySE526I	ACGT	1 cut(s)	783
Hsp92II	CATG	7 cut(s)	235, 386, 616, 628, 681, 851, 965
HspAI	GCGC	1 cut(s)	1028
Ksp22I	TGATCA	1 cut(s)	1144
Kzo9I	GATC	9 cut(s)	79, 448, 532, 829, 1015, 1021, 1110, 1127, 1144
LmnI	GCTCC	2 cut(s)	119, 323
Lsp1109I	GCAGC	4 cut(s)	266, 532, 607, 653
LweI	GCATC	1 cut(s)	1147
MaeI	CTAG	3 cut(s)	522, 563, 1177
MaeII	ACGT	1 cut(s)	783
MaeIII	GTNAC	5 cut(s)	273, 307, 605, 703, 916
MalI	GATC	9 cut(s)	81, 450, 534, 831, 1017, 1023, 1112, 1129, 1146
MbiI	CCGCTC	1 cut(s)	36
MboI	GATC	9 cut(s)	79, 448, 532, 829, 1015, 1021, 1110, 1127, 1144
MboII	GAAGA	5 cut(s)	163, 593, 770, 1108, 1166
MfeI	CAATTG	1 cut(s)	374
MflI	RGATCY	2 cut(s)	829, 1127
MhlI	GDGCHC	1 cut(s)	462
MluCI	AATT	6 cut(s)	184, 374, 630, 900, 927, 934
MlyI	GAGTC	1 cut(s)	993
MmeI	TCCRAC	2 cut(s)	832, 1119
MroXI	GAANNNNTTC	1 cut(s)	777
MseI	TTAA	3 cut(s)	62, 552, 635
MslI	CAYNNNNRTG	1 cut(s)	1047
MspI	CCGG	1 cut(s)	765
MspR9I	CCNGG	3 cut(s)	41, 464, 947
MunI	CAATTG	1 cut(s)	374
MvaI	CCWGG	3 cut(s)	41, 464, 947
MvnI	CGCG	2 cut(s)	176, 1028
MwoI	GCNNNNNNNGC	2 cut(s)	545, 626
NcoI	CCATGG	1 cut(s)	624
NdeI	CATATG	1 cut(s)	261
NdeII	GATC	9 cut(s)	79, 448, 532, 829, 1015, 1021, 1110, 1127, 1144
NlaIII	CATG	7 cut(s)	235, 386, 616, 628, 681, 851, 965
NlaIV	GGNNCC	2 cut(s)	325, 370
NmuCI	GTSAC	4 cut(s)	307, 605, 703, 916
NspI	RCATGY	1 cut(s)	681
NspV	TTCGAA	1 cut(s)	750
PdmI	GAANNNNTTC	1 cut(s)	777
PfeI	GAWTC	1 cut(s)	867
PkrI	GCNGC	6 cut(s)	38, 89, 256, 547, 622, 643
PleI	GAGTC	1 cut(s)	992
PpsI	GAGTC	1 cut(s)	992
Psp6I	CCWGG	3 cut(s)	39, 462, 945
PspGI	CCWGG	3 cut(s)	39, 462, 945
PspN4I	GGNNCC	2 cut(s)	325, 370
PsuI	RGATCY	2 cut(s)	829, 1127
RsaI	GTAC	2 cut(s)	230, 381
RsaNI	GTAC	2 cut(s)	229, 380
RseI	CAYNNNNRTG	1 cut(s)	1047
SalI	GTCGAC	1 cut(s)	395
SaqAI	TTAA	3 cut(s)	62, 552, 635
SatI	GCNGC	6 cut(s)	37, 88, 255, 546, 621, 642
Sau3AI	GATC	9 cut(s)	79, 448, 532, 829, 1015, 1021, 1110, 1127, 1144
SchI	GAGTC	1 cut(s)	993
ScrFI	CCNGG	3 cut(s)	41, 464, 947
SduI	GDGCHC	1 cut(s)	462
SetI	ASST	8 cut(s)	23, 424, 498, 547, 696, 786, 948, 954
SfaNI	GCATC	1 cut(s)	1147
SfcI	CTRYAG	2 cut(s)	157, 540
SfuI	TTCGAA	1 cut(s)	750
SmiMI	CAYNNNNRTG	1 cut(s)	1047
SmlI	CTYRAG	1 cut(s)	878
SmoI	CTYRAG	1 cut(s)	878
Sse9I	AATT	6 cut(s)	184, 374, 630, 900, 927, 934
SsiI	CCGC	5 cut(s)	36, 85, 88, 305, 506
SspI	AATATT	2 cut(s)	245, 490
SspMI	CTAG	3 cut(s)	522, 563, 1177
StyD4I	CCNGG	3 cut(s)	39, 462, 945
StyI	CCWWGG	1 cut(s)	624
TaiI	ACGT	1 cut(s)	786
TaqI	TCGA	7 cut(s)	72, 78, 149, 396, 750, 771, 870
TaqII	GACCGA	1 cut(s)	422
TasI	AATT	6 cut(s)	184, 374, 630, 900, 927, 934
TatI	WGTACW	1 cut(s)	228
TauI	GCSGC	2 cut(s)	39, 90
TfiI	GAWTC	1 cut(s)	867
Tru1I	TTAA	3 cut(s)	62, 552, 635
Tru9I	TTAA	3 cut(s)	62, 552, 635
TscAI	CASTG	2 cut(s)	607, 1088
TseFI	GTSAC	4 cut(s)	307, 605, 703, 916
TseI	GCWGC	4 cut(s)	254, 545, 620, 641
Tsp45I	GTSAC	4 cut(s)	307, 605, 703, 916
TspDTI	ATGAA	5 cut(s)	333, 582, 594, 644, 1077
TspGWI	ACGGA	2 cut(s)	675, 1106
TspRI	CASTG	2 cut(s)	607, 1088
VneI	GTGCAC	1 cut(s)	458
XapI	RAATTY	1 cut(s)	184
XceI	RCATGY	1 cut(s)	681
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1121
XmiI	GTMKAC	1 cut(s)	396
XmnI	GAANNNNTTC	1 cut(s)	777
XspI	CTAG	3 cut(s)	522, 563, 1177

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.