Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp05

Orthogroup ID

OG0002154

Physical Location & Seq

Genomic Coordinates Forward (+)

6811451 .. 6813134

Gene Structure 1684 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.5G062600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1131 bp

ATGCATAGCATAGCAAAAAGGTGGAGGCTTCTCAGCGGCGAGACTGAGTGGGAGGGTCTACTCAACCCTCTCGACCTTGATCTGCGCCGCTATATCCTTCACTACGGGGAAAGGGCTGCTGCCATAGCTGACGCCTTCATTGATGAGACAAAATCGGACAACTGCGGACTTCCTCGATATGCAAAGAGATATTTGTTCTCTAAGGTAGGTTTGGAGAATTCTAATCCATACAAGTACAAGGTGAAGAAATATTTATACGCTGCAACGACATTATCACCCGGGAAATCGATCTGGTTGGGTTACGTTGCAGTGGCAACGGATGAGGGAAAGAAAGTGTTGGGGAGGAGGGACATTTTGGTTTCATGGAGAGGAACTGAGCTGGTGGCAGAGTGGGGTATTGACGTAGTGTTCGATTTGGTTAGTGCTTCTGATATTCTGGGAGAGAAGCATGACCCTAAGGTTCACCATGGTTTCCATTCCTATTACACTCATATTGACCCTCAGTCTCCACACAACAAAACTAGTTGTAGGGCTCAGGCTTTAGCTGCAATTAAAGAAGTAGTGAACCGATACAAGAATGAGACAATCAGCATAACTGTCACCGGCCACAGCATGGGCGCTGCAATGGGAATTTTAAACGCTACAGACATTGTTTATAATGGATACAATAAGCCCCCAGGCCATCCAGTCTGTCCTGTCACAGCCATTGTGTTTGCTGCCCCTCGTCTTGGAGACCAAGGCTTAAGCAATGTGTTCTATGGTCTGAAAAATCTTCACGTCTTGCGTGTCACGAATGACTACGATTTAATCCCTTCTTTACCGCCGTTTGCAAACTATCTTCATGTCGGAAAAGAGCTGAGATTCGACACTTTCAAATCACCGTATTTGAAGGATCCGAAACAGAGCCTGCACAGTTTGGAGGTTTACTTACATGGAGTTGCAGGAACACAAGGAGAAAATGATTTTAAATTGGCAGTTAAACGTGATCTTGCATTAGTAAACAAATATTTGGACGGGCTAAAAGATAAGTATCTTGTAGTTGCTAAGTGGTGGACAGAGAAGAACAAGTCTATGCTTCAAATAGACGACGGCTCCTGGGTGTTGATGGATCATGAAAGTGATGATGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006638 GO:0006639 GO:0008150 GO:0008152 GO:0008970 GO:0009056 GO:0009987 GO:0010029 GO:0010187 GO:0010876 GO:0016042 GO:0016298 GO:0016787 GO:0016788 GO:0019915 GO:0033036 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044424 GO:0044464 GO:0046339 GO:0046340 GO:0046461 GO:0046462 GO:0046464 GO:0046486 GO:0046503 GO:0047372 GO:0048519 GO:0048580 GO:0048581 GO:0050789 GO:0050793 GO:0051093 GO:0051179 GO:0051235 GO:0051239 GO:0051241 GO:0052651 GO:0052689 GO:0065007 GO:0065008 GO:0071704 GO:1900140 GO:1901575 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lipase_3

Protein Analysis

377

Amino Acids

42.35

Weight (kDa)

7.73

Isoelectric Point (pI)

32.78

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002154)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g19750 FvH4_5g19760
malus_domestica	MD04G1058700.v1.1
prunus_persica	Prupe.5G062500_v2.0.a1 Prupe.5G062600_v2.0.a1 Prupe.5G062700_v2.0.a1
pyrus_communis	pycom04g05260
rosa_chinensis	RchiOBHm_Chr7g0203651 RchiOBHm_Chr7g0203681 RchiOBHm_Chr7g0204521 RchiOBHm_Chr7g0204541
rosa_laevigata	RLG00000003452 RLG00000003453 RLG00000003512
rosa_multiflora	Rmu_co8274427.1_g000001 Rmu_co8340383.1_g000001 Rmu_sc0005354.1_g000007 Rmu_ssc0000443.1_g000023 Rmu_ssc0000443.1_g000033
rosa_roxburghii	Rroxscaffold_3G00253010 Rroxscaffold_3G00253020 Rroxscaffold_3G00253540 Rroxscaffold_3G00253610 Rroxscaffold_6G00397050 Rroxscaffold_6G00397100
rosa_rugosa	Rorug07G0078000 Rorug07G0084900 Rorug07G0085000
rosa_samantha	Rh7AG207600 Rh7AG214200 Rh7AG214300 Rh7BG206900 Rh7BG211700 Rh7BG211800 Rh7CG219900 Rh7CG227300 Rh7DG214500 Rh7DG214700 Rh7DG221800 Rh7DG222000
rosa_wichuraiana	Rw7G018100 Rw7G018570 Rw7G018580 Rw7G018590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	657
AccB7I	CCANNNNNTGG	1 cut(s)	613
AccI	GTMKAC	1 cut(s)	58
AciI	CCGC	4 cut(s)	36, 88, 165, 821
AclWI	GGATC	3 cut(s)	887, 900, 1116
AcoI	YGGCCR	1 cut(s)	604
AcsI	RAATTY	2 cut(s)	217, 630
AcyI	GRCGYC	1 cut(s)	132
AfaI	GTAC	1 cut(s)	236
AfiI	CCNNNNNNNGG	2 cut(s)	613, 728
AflII	CTTAAG	1 cut(s)	742
AgsI	TTSAA	3 cut(s)	874, 889, 1079
AhdI	GACNNNNNGTC	1 cut(s)	502
AhlI	ACTAGT	1 cut(s)	521
AjiI	CACGTC	1 cut(s)	778
AjnI	CCWGG	2 cut(s)	676, 1094
AluBI	AGCT	4 cut(s)	128, 379, 545, 856
AluI	AGCT	4 cut(s)	128, 379, 545, 856
Alw26I	GTCTC	5 cut(s)	35, 140, 510, 575, 726
AlwI	GGATC	3 cut(s)	887, 900, 1116
AlwNI	CAGNNNCTG	1 cut(s)	907
Ama87I	CYCGRG	1 cut(s)	278
AoxI	GGCC	2 cut(s)	604, 679
ApeKI	GCWGC	6 cut(s)	116, 119, 260, 545, 620, 716
ApoI	RAATTY	2 cut(s)	217, 630
ArsI	GACNNNNNNTTYG	2 cut(s)	392, 424
AspLEI	GCGC	2 cut(s)	87, 620
AsuC2I	CCSGG	2 cut(s)	279, 280
AsuHPI	GGTGA	5 cut(s)	253, 267, 455, 592, 870
AvaI	CYCGRG	1 cut(s)	278
AxyI	CCTNAGG	1 cut(s)	456
BamHI	GGATCC	1 cut(s)	892
BanII	GRGCYC	1 cut(s)	535
BbvI	GCAGC	6 cut(s)	103, 106, 247, 532, 607, 703
BccI	CCATC	2 cut(s)	690, 1099
BceAI	ACGGC	2 cut(s)	808, 1105
BciT130I	CCWGG	2 cut(s)	678, 1096
BciVI	GTATCC	1 cut(s)	656
BcnI	CCSGG	2 cut(s)	279, 280
BcoDI	GTCTC	5 cut(s)	35, 140, 510, 575, 726
BcuI	ACTAGT	1 cut(s)	521
BfaI	CTAG	1 cut(s)	522
BfmI	CTRYAG	1 cut(s)	642
BfoI	RGCGCY	1 cut(s)	621
BfrI	CTTAAG	1 cut(s)	742
BfuI	GTATCC	1 cut(s)	656
BisI	GCNGC	8 cut(s)	37, 88, 117, 120, 261, 546, 621, 717
BlsI	GCNGC	8 cut(s)	38, 89, 118, 121, 262, 547, 622, 718
Bme1390I	CCNGG	4 cut(s)	279, 280, 678, 1096
BmeRI	GACNNNNNGTC	1 cut(s)	502
BmeT110I	CYCGRG	1 cut(s)	278
BmgBI	CACGTC	1 cut(s)	778
BmiI	GGNNCC	2 cut(s)	894, 1093
BmrFI	CCNGG	4 cut(s)	279, 280, 678, 1096
Bpu10I	CCTNAGC	1 cut(s)	534
BpuMI	CCSGG	2 cut(s)	279, 280
Bsa29I	ATCGAT	1 cut(s)	287
BsaBI	GATNNNNATC	1 cut(s)	1029
BsaHI	GRCGYC	1 cut(s)	132
BsaI	GGTCTC	1 cut(s)	726
BsaJI	CCNNGG	5 cut(s)	278, 466, 676, 736, 1095
BsaXI	ACNNNNNCTCC	2 cut(s)	1076, 1106
Bsc4I	CCNNNNNNNGG	2 cut(s)	613, 728
Bse118I	RCCGGY	1 cut(s)	602
Bse1I	ACTGG	1 cut(s)	686
Bse21I	CCTNAGG	1 cut(s)	456
Bse3DI	GCAATG	2 cut(s)	630, 754
Bse8I	GATNNNNATC	1 cut(s)	1029
BseBI	CCWGG	2 cut(s)	678, 1096
BseCI	ATCGAT	1 cut(s)	287
BseDI	CCNNGG	5 cut(s)	278, 466, 676, 736, 1095
BseGI	GGATG	2 cut(s)	325, 682
BseJI	GATNNNNATC	1 cut(s)	1029
BseLI	CCNNNNNNNGG	2 cut(s)	613, 728
BseMI	GCAATG	2 cut(s)	630, 754
BseMII	CTCAG	6 cut(s)	36, 46, 366, 515, 548, 848
BseNI	ACTGG	1 cut(s)	686
BseRI	GAGGAG	1 cut(s)	358
BseXI	GCAGC	6 cut(s)	103, 106, 247, 532, 607, 703
BsgI	GTGCAG	1 cut(s)	893
BshFI	GGCC	2 cut(s)	606, 681
BshVI	ATCGAT	1 cut(s)	287
BsiHKCI	CYCGRG	1 cut(s)	278
BsiSI	CCGG	2 cut(s)	279, 603
BslFI	GGGAC	1 cut(s)	362
BslI	CCNNNNNNNGG	2 cut(s)	613, 728
BsmAI	GTCTC	5 cut(s)	35, 140, 510, 575, 726
BsmFI	GGGAC	1 cut(s)	362
BsnI	GGCC	2 cut(s)	606, 681
Bso31I	GGTCTC	1 cut(s)	726
BsoBI	CYCGRG	1 cut(s)	278
Bsp1286I	GDGCHC	1 cut(s)	535
Bsp143I	GATC	5 cut(s)	79, 288, 892, 985, 1108
Bsp19I	CCATGG	1 cut(s)	466
BspACI	CCGC	4 cut(s)	36, 88, 165, 821
BspANI	GGCC	2 cut(s)	606, 681
BspCNI	CTCAG	6 cut(s)	37, 45, 367, 514, 547, 849
BspDI	ATCGAT	1 cut(s)	287
BspHI	TCATGA	1 cut(s)	1111
BspLI	GGNNCC	2 cut(s)	894, 1093
BspPI	GGATC	3 cut(s)	887, 900, 1116
BspTI	CTTAAG	1 cut(s)	742
BspTNI	GGTCTC	1 cut(s)	726
BsrDI	GCAATG	2 cut(s)	630, 754
BsrFI	RCCGGY	1 cut(s)	602
BsrI	ACTGG	1 cut(s)	686
BssAI	RCCGGY	1 cut(s)	602
BssECI	CCNNGG	5 cut(s)	278, 466, 676, 736, 1095
BssMI	GATC	5 cut(s)	79, 288, 892, 985, 1108
BssNI	GRCGYC	1 cut(s)	132
BssT1I	CCWWGG	2 cut(s)	466, 736
Bst2UI	CCWGG	2 cut(s)	678, 1096
Bst4CI	ACNGT	3 cut(s)	598, 882, 914
BstACI	GRCGYC	1 cut(s)	132
BstAFI	CTTAAG	1 cut(s)	742
BstC8I	GCNNGC	1 cut(s)	908
BstDEI	CTNAG	9 cut(s)	32, 45, 201, 375, 456, 501, 534, 857, 1044
BstDSI	CCRYGG	1 cut(s)	466
BstF5I	GGATG	2 cut(s)	325, 682
BstH2I	RGCGCY	1 cut(s)	621
BstHHI	GCGC	2 cut(s)	87, 620
BstKTI	GATC	5 cut(s)	82, 291, 895, 988, 1111
BstMAI	GTCTC	5 cut(s)	35, 140, 510, 575, 726
BstMBI	GATC	5 cut(s)	79, 288, 892, 985, 1108
BstMWI	GCNNNNNNNGC	2 cut(s)	125, 545
BstNI	CCWGG	2 cut(s)	678, 1096
BstSCI	CCNGG	4 cut(s)	277, 278, 676, 1094
BstSFI	CTRYAG	1 cut(s)	642
BstV1I	GCAGC	6 cut(s)	103, 106, 247, 532, 607, 703
BstX2I	RGATCY	1 cut(s)	892
BstYI	RGATCY	1 cut(s)	892
Bsu15I	ATCGAT	1 cut(s)	287
Bsu36I	CCTNAGG	1 cut(s)	456
BsuI	GTATCC	1 cut(s)	656
BsuRI	GGCC	2 cut(s)	606, 681
BsuTUI	ATCGAT	1 cut(s)	287
BtgI	CCRYGG	1 cut(s)	466
BtrI	CACGTC	1 cut(s)	778
BtsCI	GGATG	2 cut(s)	325, 682
BtsI	GCAGTG	1 cut(s)	315
BtsIMutI	CAGTG	1 cut(s)	315
Cac8I	GCNNGC	1 cut(s)	908
CaiI	CAGNNNCTG	1 cut(s)	907
CciI	TCATGA	1 cut(s)	1111
CfoI	GCGC	2 cut(s)	87, 620
Cfr10I	RCCGGY	1 cut(s)	602
Cfr9I	CCCGGG	1 cut(s)	278
ClaI	ATCGAT	1 cut(s)	287
CseI	GACGC	1 cut(s)	140
Csp6I	GTAC	1 cut(s)	235
CviAII	CATG	7 cut(s)	363, 449, 467, 613, 842, 932, 1112
CviQI	GTAC	1 cut(s)	235
DdeI	CTNAG	9 cut(s)	32, 45, 201, 375, 456, 501, 534, 857, 1044
DpnI	GATC	5 cut(s)	81, 290, 894, 987, 1110
DpnII	GATC	5 cut(s)	79, 288, 892, 985, 1108
DraI	TTTAAA	2 cut(s)	636, 967
DriI	GACNNNNNGTC	1 cut(s)	502
EaeI	YGGCCR	1 cut(s)	604
Eam1105I	GACNNNNNGTC	1 cut(s)	502
Eco130I	CCWWGG	2 cut(s)	466, 736
Eco24I	GRGCYC	1 cut(s)	535
Eco31I	GGTCTC	1 cut(s)	726
Eco81I	CCTNAGG	1 cut(s)	456
Eco88I	CYCGRG	1 cut(s)	278
EcoRI	GAATTC	1 cut(s)	217
EcoRII	CCWGG	2 cut(s)	676, 1094
EcoT14I	CCWWGG	2 cut(s)	466, 736
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	1 cut(s)	535
ErhI	CCWWGG	2 cut(s)	466, 736
FaeI	CATG	7 cut(s)	366, 452, 470, 616, 845, 935, 1115
FaqI	GGGAC	1 cut(s)	362
FatI	CATG	7 cut(s)	362, 448, 466, 612, 841, 931, 1111
FblI	GTMKAC	1 cut(s)	58
Fnu4HI	GCNGC	8 cut(s)	37, 88, 117, 120, 261, 546, 621, 717
FokI	GGATG	2 cut(s)	332, 669
FriOI	GRGCYC	1 cut(s)	535
Fsp4HI	GCNGC	8 cut(s)	37, 88, 117, 120, 261, 546, 621, 717
FspBI	CTAG	1 cut(s)	522
GlaI	GCGC	2 cut(s)	86, 619
GluI	GCNGC	8 cut(s)	37, 88, 117, 120, 261, 546, 621, 717
HaeII	RGCGCY	1 cut(s)	621
HaeIII	GGCC	2 cut(s)	606, 681
HapII	CCGG	2 cut(s)	279, 603
HgaI	GACGC	1 cut(s)	140
HhaI	GCGC	2 cut(s)	87, 620
Hin1I	GRCGYC	1 cut(s)	132
Hin1II	CATG	7 cut(s)	366, 452, 470, 616, 845, 935, 1115
Hin6I	GCGC	2 cut(s)	85, 618
HinP1I	GCGC	2 cut(s)	85, 618
HinfI	GANTC	1 cut(s)	861
HpaII	CCGG	2 cut(s)	279, 603
HphI	GGTGA	5 cut(s)	253, 267, 455, 592, 870
Hpy166II	GTNNAC	6 cut(s)	59, 463, 565, 925, 1000, 1053
Hpy188I	TCNGA	5 cut(s)	157, 430, 765, 848, 897
Hpy188III	TCNNGA	3 cut(s)	71, 790, 1112
Hpy8I	GTNNAC	6 cut(s)	59, 463, 565, 925, 1000, 1053
Hpy99I	CGWCG	1 cut(s)	1091
HpyAV	CCTTC	4 cut(s)	107, 145, 822, 883
HpyCH4III	ACNGT	3 cut(s)	598, 882, 914
HpyCH4IV	ACGT	4 cut(s)	303, 402, 777, 982
HpyF10VI	GCNNNNNNNGC	2 cut(s)	125, 545
HpyF3I	CTNAG	9 cut(s)	32, 45, 201, 375, 456, 501, 534, 857, 1044
HpySE526I	ACGT	4 cut(s)	303, 402, 777, 982
Hsp92I	GRCGYC	1 cut(s)	132
Hsp92II	CATG	7 cut(s)	366, 452, 470, 616, 845, 935, 1115
HspAI	GCGC	2 cut(s)	85, 618
Kzo9I	GATC	5 cut(s)	79, 288, 892, 985, 1108
LmnI	GCTCC	1 cut(s)	1097
Lsp1109I	GCAGC	6 cut(s)	103, 106, 247, 532, 607, 703
MaeI	CTAG	1 cut(s)	522
MaeII	ACGT	4 cut(s)	303, 402, 777, 982
MaeIII	GTNAC	4 cut(s)	299, 598, 697, 787
MalI	GATC	5 cut(s)	81, 290, 894, 987, 1110
MboI	GATC	5 cut(s)	79, 288, 892, 985, 1108
MboII	GAAGA	4 cut(s)	256, 764, 830, 1072
MflI	RGATCY	1 cut(s)	892
MhlI	GDGCHC	1 cut(s)	535
MluCI	AATT	4 cut(s)	217, 549, 630, 968
MmeI	TCCRAC	1 cut(s)	826
Mph1103I	ATGCAT	1 cut(s)	6
MseI	TTAA	6 cut(s)	552, 635, 743, 806, 966, 978
MslI	CAYNNNNRTG	1 cut(s)	1116
MspA1I	CMGCKG	1 cut(s)	36
MspCI	CTTAAG	1 cut(s)	742
MspI	CCGG	2 cut(s)	279, 603
MspR9I	CCNGG	4 cut(s)	279, 280, 678, 1096
MvaI	CCWGG	2 cut(s)	678, 1096
MwoI	GCNNNNNNNGC	2 cut(s)	125, 545
NciI	CCSGG	2 cut(s)	279, 280
NcoI	CCATGG	1 cut(s)	466
NdeII	GATC	5 cut(s)	79, 288, 892, 985, 1108
NlaIII	CATG	7 cut(s)	366, 452, 470, 616, 845, 935, 1115
NlaIV	GGNNCC	2 cut(s)	894, 1093
NmuCI	GTSAC	3 cut(s)	598, 697, 787
NsiI	ATGCAT	1 cut(s)	6
PagI	TCATGA	1 cut(s)	1111
PcsI	WCGNNNNNNNCGW	1 cut(s)	408
PfeI	GAWTC	1 cut(s)	861
PflMI	CCANNNNNTGG	1 cut(s)	613
PkrI	GCNGC	8 cut(s)	38, 89, 118, 121, 262, 547, 622, 718
PsiI	TTATAA	1 cut(s)	657
Psp6I	CCWGG	2 cut(s)	676, 1094
PspGI	CCWGG	2 cut(s)	676, 1094
PspN4I	GGNNCC	2 cut(s)	894, 1093
PstNI	CAGNNNCTG	1 cut(s)	907
PsuI	RGATCY	1 cut(s)	892
RsaI	GTAC	1 cut(s)	236
RsaNI	GTAC	1 cut(s)	235
RseI	CAYNNNNRTG	1 cut(s)	1116
SaqAI	TTAA	6 cut(s)	552, 635, 743, 806, 966, 978
SatI	GCNGC	8 cut(s)	37, 88, 117, 120, 261, 546, 621, 717
Sau3AI	GATC	5 cut(s)	79, 288, 892, 985, 1108
ScrFI	CCNGG	4 cut(s)	279, 280, 678, 1096
SduI	GDGCHC	1 cut(s)	535
SfcI	CTRYAG	1 cut(s)	642
SmaI	CCCGGG	1 cut(s)	280
SmiMI	CAYNNNNRTG	1 cut(s)	1116
SmlI	CTYRAG	1 cut(s)	742
SmoI	CTYRAG	1 cut(s)	742
SpeI	ACTAGT	1 cut(s)	521
Sse9I	AATT	4 cut(s)	217, 549, 630, 968
SsiI	CCGC	4 cut(s)	36, 88, 165, 821
SspI	AATATT	2 cut(s)	251, 1007
SspMI	CTAG	1 cut(s)	522
StyD4I	CCNGG	4 cut(s)	277, 278, 676, 1094
StyI	CCWWGG	2 cut(s)	466, 736
TaaI	ACNGT	3 cut(s)	598, 882, 914
TaiI	ACGT	4 cut(s)	306, 405, 780, 985
TaqI	TCGA	5 cut(s)	72, 175, 287, 411, 864
TasI	AATT	4 cut(s)	217, 549, 630, 968
TatI	WGTACW	1 cut(s)	234
TauI	GCSGC	2 cut(s)	39, 90
TfiI	GAWTC	1 cut(s)	861
Tru1I	TTAA	6 cut(s)	552, 635, 743, 806, 966, 978
Tru9I	TTAA	6 cut(s)	552, 635, 743, 806, 966, 978
TscAI	CASTG	1 cut(s)	315
TseFI	GTSAC	3 cut(s)	598, 697, 787
TseI	GCWGC	6 cut(s)	116, 119, 260, 545, 620, 716
Tsp45I	GTSAC	3 cut(s)	598, 697, 787
TspDTI	ATGAA	4 cut(s)	127, 351, 830, 1128
TspGWI	ACGGA	1 cut(s)	332
TspMI	CCCGGG	1 cut(s)	278
TspRI	CASTG	1 cut(s)	315
Van91I	CCANNNNNTGG	1 cut(s)	613
Vha464I	CTTAAG	1 cut(s)	742
XapI	RAATTY	2 cut(s)	217, 630
XmaI	CCCGGG	1 cut(s)	278
XmiI	GTMKAC	1 cut(s)	58
XspI	CTAG	1 cut(s)	522
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.