Rh7AG214300

phospholipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0002154
Physical Location & Seq
Reverse (-)
19818761 .. 19820158
1398 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG214300.1

Sequence Viewer

Length: 1167 bp
ATGGCTAGCTTTGCAGAAAACTGGAGGGATCTGAGCGGCAAGAACGACTGGGAGGGTCTACTAGACCCTCTCGACAGCAATCTCCGCCGCGCTATCATTCACTATGGTGAAAGGGCTGGAGCGGCGATATTCGTGGTGAAGGAGCCAAAGTCGAAGAACTACAGACTTCCGCAATACGCAAGAGGACATTTGTTCGCAAAGGTGGGTTTGGAGAGTGGTAATCCATACAAGTACATGGTGAAAAAATATTTTTATGCAACTCATCTGGAACTTTCGCCCAAGAAATCAAACTTCCTGGGTTTTGTTGCGGTGACAACTGATGAAGGAACCAAAGTGCTAGGCCGGAGGGATGTCTTGATTTCATGGAGAGGAACCACATTGGTACATGAGTGGCTTGTTGACGCGAACATCAATTTGGTCTCAGCTTCTCACATTCTCGGAGTGGAGAATGATCCTAAGGTGCACCAGGGTTGGCTTTCCTATTACACTGATATGGACCCTGAGTCCTCACACAATCAACTTACTAGTTCCAGAGATCAGGCTCTAGCTGCACTTAAAGAAGTACTTGATGAATACAAGGGTGAAGAAATCAGCATTACTGTCACTGGTCACAGCATGGGTGCTGCACTCGCAATTCTAAATGCGTCTGATATTGTTCATAACGGATACAATAAAATGACAGATCAACCAAATAAGGTCATTCCTGTCACAGCTATTGTGTTTGCCTGCCCTCGTCTTGGAAACCAGGGTTTTAAAATTGTACTTTCTGGTCTTGAAAATCTTCACGTCTTGCGTGTCACAAATGACCAAGATATCGTCCCTAAAGCACCAGAATCGGTGGGTTATGTTCATGTCGGAAAAGAGTTGAGAATCGACACTCTCAAGTCGCCGTTCTTGAAAGTTATTCCTGATACTAATGTCACAGAAAAACTTATCCATTTGCACGATCTGGAGGCTTACTTGCATGGAGTTGCAGGGACACAGGGGATAAAAAGTTCTGATTTCAAATTGGAAGTTGATCGTGATCTGGCACTGGTAAACAAGTTTATTGATGGCGTGAAGGATGAACATAATATAGTTGCTCAGTGGTGGACTGAGAAGAATAAGTCTATGATCCAAATGGATGATGGCTCTTGGGTGTTGGATGATCACGAGGAAGATGAATAG

Protein Analysis

388

Amino Acids

43.5

Weight (kDa)

5.74

Isoelectric Point (pI)

29.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 120 - 278 1.4e-32 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 36, 122
AccI GTMKAC 1 cut(s) 58
AccII CGCG 2 cut(s) 90, 404
AciI CCGC 6 cut(s) 36, 85, 88, 122, 170, 308
AclWI GGATC 3 cut(s) 36, 446, 1108
AfaI GTAC 4 cut(s) 233, 384, 564, 762
AfiI CCNNNNNNNGG 1 cut(s) 737
AgsI TTSAA 3 cut(s) 776, 898, 1006
AhdI GACNNNNNGTC 1 cut(s) 502
AhlI ACTAGT 1 cut(s) 524
AjiI CACGTC 1 cut(s) 787
AjnI CCWGG 3 cut(s) 294, 465, 744
AjuI GAANNNNNNNTTGG 2 cut(s) 398, 430
AleI CACNNNNGTG 1 cut(s) 105
AloI GAACNNNNNNTCC 2 cut(s) 979, 1011
AluBI AGCT 4 cut(s) 9, 425, 548, 713
AluI AGCT 4 cut(s) 9, 425, 548, 713
Alw21I GWGCWC 1 cut(s) 465
Alw26I GTCTC 1 cut(s) 424
Alw44I GTGCAC 1 cut(s) 461
AlwI GGATC 3 cut(s) 36, 446, 1108
AoxI GGCC 1 cut(s) 340
ApaLI GTGCAC 1 cut(s) 461
ApeKI GCWGC 2 cut(s) 548, 623
Asp700I GAANNNNTTC 1 cut(s) 780
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 1 cut(s) 496
AsuHPI GGTGA 5 cut(s) 119, 148, 250, 322, 593
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 1 cut(s) 496
AxyI CCTNAGG 1 cut(s) 456
BaeGI GKGCMC 1 cut(s) 465
BaeI ACNNNNGTAYC 4 cut(s) 374, 407, 903, 936
BauI CACGAG 1 cut(s) 1151
Bbv12I GWGCWC 1 cut(s) 465
BbvI GCAGC 2 cut(s) 535, 610
BccI CCATC 2 cut(s) 1046, 1121
BceAI ACGGC 1 cut(s) 874
BcgI CGANNNNNNTGC 2 cut(s) 816, 850
BciT130I CCWGG 3 cut(s) 296, 467, 746
BciVI GTATCC 1 cut(s) 659
BclI TGATCA 1 cut(s) 1147
BcoDI GTCTC 1 cut(s) 424
BcuI ACTAGT 1 cut(s) 524
BfaI CTAG 5 cut(s) 6, 62, 338, 525, 545
BfmI CTRYAG 1 cut(s) 160
BfuI GTATCC 1 cut(s) 659
BisI GCNGC 5 cut(s) 37, 88, 123, 549, 624
BlsI GCNGC 5 cut(s) 38, 89, 124, 550, 625
BmcAI AGTACT 1 cut(s) 564
Bme1390I CCNGG 3 cut(s) 296, 467, 746
Bme18I GGWCC 1 cut(s) 496
BmeRI GACNNNNNGTC 1 cut(s) 502
BmgBI CACGTC 1 cut(s) 787
BmgT120I GGNCC 1 cut(s) 496
BmiI GGNNCC 4 cut(s) 144, 328, 373, 498
BmrFI CCNGG 3 cut(s) 296, 467, 746
BmrI ACTGGG 1 cut(s) 58
BmtI GCTAGC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 58
BpmI CTGGAG 3 cut(s) 43, 138, 971
BpuEI CTTGAG 1 cut(s) 866
BsaBI GATNNNNATC 1 cut(s) 1023
BsaI GGTCTC 1 cut(s) 424
BsaJI CCNNGG 3 cut(s) 295, 466, 745
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 1 cut(s) 737
Bse1I ACTGG 4 cut(s) 26, 53, 610, 1038
Bse21I CCTNAGG 1 cut(s) 456
Bse8I GATNNNNATC 1 cut(s) 1023
BseBI CCWGG 3 cut(s) 296, 467, 746
BseDI CCNNGG 3 cut(s) 295, 466, 745
BseGI GGATG 4 cut(s) 355, 1069, 1129, 1150
BseJI GATNNNNATC 1 cut(s) 1023
BseLI CCNNNNNNNGG 1 cut(s) 737
BseMII CTCAG 5 cut(s) 23, 435, 492, 1086, 1097
BseNI ACTGG 4 cut(s) 26, 53, 610, 1038
BseSI GKGCMC 1 cut(s) 465
BseXI GCAGC 2 cut(s) 535, 610
BsgI GTGCAG 2 cut(s) 534, 609
Bsh1236I CGCG 2 cut(s) 90, 404
BshFI GGCC 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 465
BsiSI CCGG 1 cut(s) 343
BslFI GGGAC 2 cut(s) 803, 991
BslI CCNNNNNNNGG 1 cut(s) 737
BsmAI GTCTC 1 cut(s) 424
BsmFI GGGAC 2 cut(s) 803, 991
BsnI GGCC 1 cut(s) 342
Bso31I GGTCTC 1 cut(s) 424
Bsp1286I GDGCHC 1 cut(s) 465
Bsp143I GATC 9 cut(s) 28, 451, 535, 682, 946, 1018, 1024, 1113, 1147
BspACI CCGC 6 cut(s) 36, 85, 88, 122, 170, 308
BspANI GGCC 1 cut(s) 342
BspCNI CTCAG 5 cut(s) 24, 434, 493, 1087, 1096
BspFNI CGCG 2 cut(s) 90, 404
BspLI GGNNCC 4 cut(s) 144, 328, 373, 498
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 3 cut(s) 36, 446, 1108
BspTNI GGTCTC 1 cut(s) 424
BsrBI CCGCTC 2 cut(s) 36, 122
BsrI ACTGG 4 cut(s) 26, 53, 610, 1038
BssECI CCNNGG 3 cut(s) 295, 466, 745
BssMI GATC 9 cut(s) 28, 451, 535, 682, 946, 1018, 1024, 1113, 1147
BssSI CACGAG 1 cut(s) 1151
Bst2BI CACGAG 1 cut(s) 1151
Bst2UI CCWGG 3 cut(s) 296, 467, 746
Bst4CI ACNGT 1 cut(s) 601
BstC8I GCNNGC 2 cut(s) 7, 727
BstDEI CTNAG 6 cut(s) 32, 421, 456, 501, 1083, 1095
BstF5I GGATG 4 cut(s) 355, 1069, 1129, 1150
BstFNI CGCG 2 cut(s) 90, 404
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 9 cut(s) 31, 454, 538, 685, 949, 1021, 1027, 1116, 1150
BstMAI GTCTC 1 cut(s) 424
BstMBI GATC 9 cut(s) 28, 451, 535, 682, 946, 1018, 1024, 1113, 1147
BstMWI GCNNNNNNNGC 5 cut(s) 11, 84, 122, 548, 629
BstNI CCWGG 3 cut(s) 296, 467, 746
BstSCI CCNGG 3 cut(s) 294, 465, 744
BstSFI CTRYAG 1 cut(s) 160
BstSLI GKGCMC 1 cut(s) 465
BstUI CGCG 2 cut(s) 90, 404
BstV1I GCAGC 2 cut(s) 535, 610
BstX2I RGATCY 1 cut(s) 28
BstYI RGATCY 1 cut(s) 28
Bsu36I CCTNAGG 1 cut(s) 456
BsuI GTATCC 1 cut(s) 659
BsuRI GGCC 1 cut(s) 342
BtrI CACGTC 1 cut(s) 787
BtsCI GGATG 4 cut(s) 355, 1069, 1129, 1150
BtsIMutI CAGTG 4 cut(s) 486, 603, 1031, 1091
Cac8I GCNNGC 2 cut(s) 7, 727
CfoI GCGC 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 496
CseI GACGC 2 cut(s) 410, 633
Csp6I GTAC 4 cut(s) 232, 383, 563, 761
CviAII CATG 6 cut(s) 235, 363, 386, 616, 851, 965
CviQI GTAC 4 cut(s) 232, 383, 563, 761
DdeI CTNAG 6 cut(s) 32, 421, 456, 501, 1083, 1095
DpnI GATC 9 cut(s) 30, 453, 537, 684, 948, 1020, 1026, 1115, 1149
DpnII GATC 9 cut(s) 28, 451, 535, 682, 946, 1018, 1024, 1113, 1147
DraI TTTAAA 1 cut(s) 754
DriI GACNNNNNGTC 1 cut(s) 502
Eam1105I GACNNNNNGTC 1 cut(s) 502
EciI GGCGGA 1 cut(s) 74
Eco31I GGTCTC 1 cut(s) 424
Eco32I GATATC 1 cut(s) 814
Eco47I GGWCC 1 cut(s) 496
Eco81I CCTNAGG 1 cut(s) 456
EcoRII CCWGG 3 cut(s) 294, 465, 744
EcoRV GATATC 1 cut(s) 814
FaeI CATG 6 cut(s) 238, 366, 389, 619, 854, 968
FalI AAGNNNNNCTT 2 cut(s) 549, 581
FaqI GGGAC 2 cut(s) 803, 991
FatI CATG 6 cut(s) 234, 362, 385, 615, 850, 964
FbaI TGATCA 1 cut(s) 1147
FblI GTMKAC 1 cut(s) 58
Fnu4HI GCNGC 5 cut(s) 37, 88, 123, 549, 624
FokI GGATG 4 cut(s) 362, 1076, 1136, 1157
Fsp4HI GCNGC 5 cut(s) 37, 88, 123, 549, 624
FspBI CTAG 5 cut(s) 6, 62, 338, 525, 545
GlaI GCGC 1 cut(s) 91
GluI GCNGC 5 cut(s) 37, 88, 123, 549, 624
GsuI CTGGAG 3 cut(s) 43, 138, 971
HaeIII GGCC 1 cut(s) 342
HapII CCGG 1 cut(s) 343
HgaI GACGC 2 cut(s) 410, 633
HhaI GCGC 1 cut(s) 92
Hin1II CATG 6 cut(s) 238, 366, 389, 619, 854, 968
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HinfI GANTC 3 cut(s) 503, 833, 870
HpaII CCGG 1 cut(s) 343
HphI GGTGA 5 cut(s) 119, 148, 250, 322, 593
Hpy166II GTNNAC 5 cut(s) 59, 400, 463, 1039, 1092
Hpy188I TCNGA 5 cut(s) 33, 440, 649, 857, 1000
Hpy8I GTNNAC 5 cut(s) 59, 400, 463, 1039, 1092
HpyAV CCTTC 3 cut(s) 133, 317, 1054
HpyCH4III ACNGT 1 cut(s) 601
HpyCH4IV ACGT 1 cut(s) 786
HpyCH4V TGCA 8 cut(s) 14, 257, 463, 551, 626, 943, 964, 974
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 84, 122, 548, 629
HpyF3I CTNAG 6 cut(s) 32, 421, 456, 501, 1083, 1095
HpySE526I ACGT 1 cut(s) 786
Hsp92II CATG 6 cut(s) 238, 366, 389, 619, 854, 968
HspAI GCGC 1 cut(s) 90
Ksp22I TGATCA 1 cut(s) 1147
Kzo9I GATC 9 cut(s) 28, 451, 535, 682, 946, 1018, 1024, 1113, 1147
LmnI GCTCC 2 cut(s) 119, 142
Lsp1109I GCAGC 2 cut(s) 535, 610
MaeI CTAG 5 cut(s) 6, 62, 338, 525, 545
MaeII ACGT 1 cut(s) 786
MaeIII GTNAC 6 cut(s) 310, 601, 608, 706, 796, 919
MalI GATC 9 cut(s) 30, 453, 537, 684, 948, 1020, 1026, 1115, 1149
MbiI CCGCTC 2 cut(s) 36, 122
MboI GATC 9 cut(s) 28, 451, 535, 682, 946, 1018, 1024, 1113, 1147
MboII GAAGA 4 cut(s) 166, 596, 773, 1111
MflI RGATCY 1 cut(s) 28
MhlI GDGCHC 1 cut(s) 465
MluCI AATT 4 cut(s) 412, 633, 756, 1007
MlyI GAGTC 1 cut(s) 512
MmeI TCCRAC 2 cut(s) 835, 1122
MroXI GAANNNNTTC 1 cut(s) 780
MseI TTAA 2 cut(s) 555, 753
MslI CAYNNNNRTG 2 cut(s) 105, 491
MspI CCGG 1 cut(s) 343
MspR9I CCNGG 3 cut(s) 296, 467, 746
MvaI CCWGG 3 cut(s) 296, 467, 746
MvnI CGCG 2 cut(s) 90, 404
MwoI GCNNNNNNNGC 5 cut(s) 11, 84, 122, 548, 629
NdeII GATC 9 cut(s) 28, 451, 535, 682, 946, 1018, 1024, 1113, 1147
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 6 cut(s) 238, 366, 389, 619, 854, 968
NlaIV GGNNCC 4 cut(s) 144, 328, 373, 498
NmuCI GTSAC 6 cut(s) 310, 601, 608, 706, 796, 919
OliI CACNNNNGTG 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 780
PfeI GAWTC 2 cut(s) 833, 870
PkrI GCNGC 5 cut(s) 38, 89, 124, 550, 625
PleI GAGTC 1 cut(s) 511
PpsI GAGTC 1 cut(s) 511
Psp6I CCWGG 3 cut(s) 294, 465, 744
PspGI CCWGG 3 cut(s) 294, 465, 744
PspN4I GGNNCC 4 cut(s) 144, 328, 373, 498
PspPI GGNCC 1 cut(s) 496
PsuI RGATCY 1 cut(s) 28
RsaI GTAC 4 cut(s) 233, 384, 564, 762
RsaNI GTAC 4 cut(s) 232, 383, 563, 761
RseI CAYNNNNRTG 2 cut(s) 105, 491
SaqAI TTAA 2 cut(s) 555, 753
SatI GCNGC 5 cut(s) 37, 88, 123, 549, 624
Sau3AI GATC 9 cut(s) 28, 451, 535, 682, 946, 1018, 1024, 1113, 1147
Sau96I GGNCC 1 cut(s) 496
ScaI AGTACT 1 cut(s) 564
SchI GAGTC 1 cut(s) 512
ScrFI CCNGG 3 cut(s) 296, 467, 746
SduI GDGCHC 1 cut(s) 465
SetI ASST 8 cut(s) 11, 204, 427, 462, 550, 699, 715, 789
SfcI CTRYAG 1 cut(s) 160
SinI GGWCC 1 cut(s) 496
SmiMI CAYNNNNRTG 2 cut(s) 105, 491
SmlI CTYRAG 1 cut(s) 881
SmoI CTYRAG 1 cut(s) 881
SpeI ACTAGT 1 cut(s) 524
Sse9I AATT 4 cut(s) 412, 633, 756, 1007
SsiI CCGC 6 cut(s) 36, 85, 88, 122, 170, 308
SspI AATATT 1 cut(s) 248
SspMI CTAG 5 cut(s) 6, 62, 338, 525, 545
StyD4I CCNGG 3 cut(s) 294, 465, 744
TaaI ACNGT 1 cut(s) 601
TaiI ACGT 1 cut(s) 789
TaqI TCGA 3 cut(s) 72, 152, 873
TasI AATT 4 cut(s) 412, 633, 756, 1007
TatI WGTACW 3 cut(s) 231, 562, 760
TauI GCSGC 3 cut(s) 39, 90, 125
TfiI GAWTC 2 cut(s) 833, 870
Tru1I TTAA 2 cut(s) 555, 753
Tru9I TTAA 2 cut(s) 555, 753
TscAI CASTG 4 cut(s) 493, 610, 1038, 1091
TseFI GTSAC 6 cut(s) 310, 601, 608, 706, 796, 919
TseI GCWGC 2 cut(s) 548, 623
Tsp45I GTSAC 6 cut(s) 310, 601, 608, 706, 796, 919
TspDTI ATGAA 6 cut(s) 336, 351, 585, 647, 839, 1080
TspGWI ACGGA 1 cut(s) 678
TspRI CASTG 4 cut(s) 493, 610, 1038, 1091
VneI GTGCAC 1 cut(s) 461
VpaK11BI GGWCC 1 cut(s) 496
XcmI CCANNNNNNNNNTGG 1 cut(s) 1124
XmiI GTMKAC 1 cut(s) 58
XmnI GAANNNNTTC 1 cut(s) 780
XspI CTAG 5 cut(s) 6, 62, 338, 525, 545
ZrmI AGTACT 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.