FvH4_5g20420

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000481
Physical Location & Seq
Reverse (-)
12075366 .. 12076699
1334 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20420.t1

Sequence Viewer

Length: 939 bp
ATGGCTGCAATAGTTGTTCAAGCCCTCGAGACCTCAAATGTCACAATTAGCATCAAATCAGTAGCTGAATCACAAGCTCTCAACTCTGTTCTTTCTGCTCATGCCTTCAGCATAAACCCCAACGATGAAGCTGATCCAAATGACCCTGAGTTTGCTGTTCCCATTGTTGATATGTCTCTTCTCACCTCCGGTTCACCTGATCAACGCGCAAAGATGGCGATCTTGTCAAAATTTGCGATTAACCACGGAGTACCAGATAGCCTGATGAAGGGGATGATTGATGCATATCATGGATTCTTTAGACTTCCAGATGAAGAGGAGGAGGAGTTTAAGTCAGGAAATGATGTTCTTGAGATGTTCAAGTATGGGACAAGCTTTAATCTGGCATTGAACAAAGTACTTCTGTGGAGGGACTTCCTCAAGGTCCAAACACATCCCGAGTTCTACTCCCTCAACAAACCAGCTCGCTTCAGTGAGGTTTCGCTGGAGTTCAGCAGAAGAACCCGAGACGTAGCATTGGAAATAACAAGAGCAATATCAGAAAGTTTAGGGTTGGAGCCGGACTACATATATAACACAATGAACATGGATCACGGCATGCAAATGCTAGTCGGAAACTACTACCCGCCTTGTCCACAGCCTGAACATGCCCTTGGTGTCGCCCATCATACCGATCATGGTCTAGTCACACTCCTCATCCAGAATGAGATGAATGGTCTCCAAGTTGAGCACAATGGGAAATGGCTCGCCGTCGATGGCGCTCCCAACGGCTTCTTCGTTAACCTCTCTGATCAATTGCATATTCTTACAAATGGTAAGTACAAGAGTGTAAAGCATCGAGCCACATTGAACAACAAAACTACAAGGATATCGATAGCTATACCACATGGACCATCTGTAGACACGATCGTGAGCCGGCAGCAGAGTTACGTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

313

Amino Acids

34.71

Weight (kDa)

5.59

Isoelectric Point (pI)

31.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 79 - 148 1.8e-10 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 201 - 295 1.3e-29 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 900
AccII CGCG 1 cut(s) 207
AciI CCGC 1 cut(s) 626
AclWI GGATC 2 cut(s) 128, 597
AcsI RAATTY 1 cut(s) 230
AcuI CTGAAG 2 cut(s) 91, 454
AfaI GTAC 3 cut(s) 252, 399, 821
AfiI CCNNNNNNNGG 1 cut(s) 268
AgsI TTSAA 4 cut(s) 20, 361, 391, 850
AjuI GAANNNNNNNTTGG 4 cut(s) 636, 668, 758, 790
AleI CACNNNNGTG 1 cut(s) 908
AloI GAACNNNNNNTCC 2 cut(s) 330, 362
AluBI AGCT 6 cut(s) 65, 77, 131, 375, 464, 878
AluI AGCT 6 cut(s) 65, 77, 131, 375, 464, 878
Alw21I GWGCWC 1 cut(s) 732
Alw26I GTCTC 4 cut(s) 23, 180, 501, 722
AlwI GGATC 2 cut(s) 128, 597
AlwNI CAGNNNCTG 1 cut(s) 65
Ama87I CYCGRG 3 cut(s) 26, 437, 504
ApeKI GCWGC 2 cut(s) 5, 919
ApoI RAATTY 1 cut(s) 230
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AspLEI GCGC 2 cut(s) 209, 761
AspS9I GGNCC 2 cut(s) 424, 890
AsuHPI GGTGA 2 cut(s) 175, 186
AvaI CYCGRG 3 cut(s) 26, 437, 504
AvaII GGWCC 2 cut(s) 424, 890
Bbv12I GWGCWC 1 cut(s) 732
BbvI GCAGC 1 cut(s) 931
BccI CCATC 4 cut(s) 208, 672, 749, 901
BceAI ACGGC 3 cut(s) 610, 734, 784
BclI TGATCA 2 cut(s) 199, 790
BcoDI GTCTC 4 cut(s) 23, 180, 501, 722
BfaI CTAG 2 cut(s) 608, 683
BfmI CTRYAG 1 cut(s) 897
BfoI RGCGCY 1 cut(s) 762
BisI GCNGC 2 cut(s) 6, 920
BlsI GCNGC 2 cut(s) 7, 921
BmcAI AGTACT 1 cut(s) 399
Bme18I GGWCC 2 cut(s) 424, 890
BmeT110I CYCGRG 3 cut(s) 26, 437, 504
BmgT120I GGNCC 2 cut(s) 424, 890
BmiI GGNNCC 1 cut(s) 558
BmsI GCATC 3 cut(s) 60, 271, 844
BplI GAGNNNNNCTC 2 cut(s) 431, 463
BpmI CTGGAG 1 cut(s) 506
BpuEI CTTGAG 2 cut(s) 371, 404
Bsa29I ATCGAT 1 cut(s) 872
BsaBI GATNNNNATC 2 cut(s) 218, 285
BsaI GGTCTC 2 cut(s) 23, 722
BsaJI CCNNGG 2 cut(s) 244, 652
BsaWI WCCGGW 1 cut(s) 188
BsaXI ACNNNNNCTCC 4 cut(s) 311, 317, 341, 347
Bsc4I CCNNNNNNNGG 1 cut(s) 268
Bse118I RCCGGY 1 cut(s) 915
Bse8I GATNNNNATC 2 cut(s) 218, 285
BseCI ATCGAT 1 cut(s) 872
BseDI CCNNGG 2 cut(s) 244, 652
BseGI GGATG 3 cut(s) 279, 433, 696
BseJI GATNNNNATC 2 cut(s) 218, 285
BseLI CCNNNNNNNGG 1 cut(s) 268
BseMII CTCAG 1 cut(s) 138
BseRI GAGGAG 4 cut(s) 332, 335, 338, 683
BseXI GCAGC 1 cut(s) 931
Bsh1236I CGCG 1 cut(s) 207
Bsh1285I CGRYCG 1 cut(s) 909
BshVI ATCGAT 1 cut(s) 872
BsiEI CGRYCG 1 cut(s) 909
BsiHKAI GWGCWC 1 cut(s) 732
BsiHKCI CYCGRG 3 cut(s) 26, 437, 504
BsiSI CCGG 3 cut(s) 189, 560, 916
BslFI GGGAC 2 cut(s) 382, 425
BslI CCNNNNNNNGG 1 cut(s) 268
BsmAI GTCTC 4 cut(s) 23, 180, 501, 722
BsmBI CGTCTC 1 cut(s) 501
BsmFI GGGAC 2 cut(s) 382, 425
Bso31I GGTCTC 2 cut(s) 23, 722
BsoBI CYCGRG 3 cut(s) 26, 437, 504
Bsp1286I GDGCHC 1 cut(s) 732
Bsp143I GATC 7 cut(s) 133, 199, 219, 589, 673, 790, 906
BspACI CCGC 1 cut(s) 626
BspCNI CTCAG 1 cut(s) 139
BspDI ATCGAT 1 cut(s) 872
BspFNI CGCG 1 cut(s) 207
BspLI GGNNCC 1 cut(s) 558
BspPI GGATC 2 cut(s) 128, 597
BspTNI GGTCTC 2 cut(s) 23, 722
BsrFI RCCGGY 1 cut(s) 915
BssAI RCCGGY 1 cut(s) 915
BssECI CCNNGG 2 cut(s) 244, 652
BssMI GATC 7 cut(s) 133, 199, 219, 589, 673, 790, 906
BssT1I CCWWGG 1 cut(s) 652
Bst6I CTCTTC 2 cut(s) 183, 309
BstC8I GCNNGC 4 cut(s) 466, 599, 747, 917
BstDEI CTNAG 1 cut(s) 147
BstDSI CCRYGG 1 cut(s) 244
BstENI CCTNNNNNAGG 1 cut(s) 266
BstF5I GGATG 3 cut(s) 279, 433, 696
BstFNI CGCG 1 cut(s) 207
BstH2I RGCGCY 1 cut(s) 762
BstHHI GCGC 2 cut(s) 209, 761
BstKTI GATC 7 cut(s) 136, 202, 222, 592, 676, 793, 909
BstMAI GTCTC 4 cut(s) 23, 180, 501, 722
BstMBI GATC 7 cut(s) 133, 199, 219, 589, 673, 790, 906
BstMCI CGRYCG 1 cut(s) 909
BstMWI GCNNNNNNNGC 1 cut(s) 215
BstNSI RCATGY 2 cut(s) 601, 650
BstSFI CTRYAG 1 cut(s) 897
BstUI CGCG 1 cut(s) 207
BstV1I GCAGC 1 cut(s) 931
Bsu15I ATCGAT 1 cut(s) 872
BsuTUI ATCGAT 1 cut(s) 872
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 3 cut(s) 279, 433, 696
BtsIMutI CAGTG 1 cut(s) 478
Cac8I GCNNGC 4 cut(s) 466, 599, 747, 917
CaiI CAGNNNCTG 1 cut(s) 65
CfoI GCGC 2 cut(s) 209, 761
Cfr10I RCCGGY 1 cut(s) 915
Cfr13I GGNCC 2 cut(s) 424, 890
ClaI ATCGAT 1 cut(s) 872
Csp6I GTAC 3 cut(s) 251, 398, 820
CviAII CATG 7 cut(s) 101, 290, 586, 598, 647, 677, 887
CviQI GTAC 3 cut(s) 251, 398, 820
DdeI CTNAG 1 cut(s) 147
DpnI GATC 7 cut(s) 135, 201, 221, 591, 675, 792, 908
DpnII GATC 7 cut(s) 133, 199, 219, 589, 673, 790, 906
Eam1104I CTCTTC 2 cut(s) 183, 309
EarI CTCTTC 2 cut(s) 183, 309
Eco130I CCWWGG 1 cut(s) 652
Eco31I GGTCTC 2 cut(s) 23, 722
Eco32I GATATC 1 cut(s) 870
Eco47I GGWCC 2 cut(s) 424, 890
Eco57I CTGAAG 2 cut(s) 91, 454
Eco88I CYCGRG 3 cut(s) 26, 437, 504
EcoNI CCTNNNNNAGG 1 cut(s) 266
EcoRV GATATC 1 cut(s) 870
EcoT14I CCWWGG 1 cut(s) 652
EcoT22I ATGCAT 1 cut(s) 286
ErhI CCWWGG 1 cut(s) 652
Esp3I CGTCTC 1 cut(s) 501
FaeI CATG 7 cut(s) 104, 293, 589, 601, 650, 680, 890
FaqI GGGAC 2 cut(s) 382, 425
FatI CATG 7 cut(s) 100, 289, 585, 597, 646, 676, 886
FauI CCCGC 1 cut(s) 633
FbaI TGATCA 2 cut(s) 199, 790
FblI GTMKAC 1 cut(s) 900
Fnu4HI GCNGC 2 cut(s) 6, 920
FokI GGATG 3 cut(s) 286, 420, 683
Fsp4HI GCNGC 2 cut(s) 6, 920
FspBI CTAG 2 cut(s) 608, 683
GlaI GCGC 2 cut(s) 208, 760
GluI GCNGC 2 cut(s) 6, 920
GsuI CTGGAG 1 cut(s) 506
HaeII RGCGCY 1 cut(s) 762
HapII CCGG 3 cut(s) 189, 560, 916
HhaI GCGC 2 cut(s) 209, 761
Hin1II CATG 7 cut(s) 104, 293, 589, 601, 650, 680, 890
Hin6I GCGC 2 cut(s) 207, 759
HinP1I GCGC 2 cut(s) 207, 759
HincII GTYRAC 1 cut(s) 781
HindII GTYRAC 1 cut(s) 781
HindIII AAGCTT 1 cut(s) 373
HinfI GANTC 2 cut(s) 68, 294
HpaI GTTAAC 1 cut(s) 781
HpaII CCGG 3 cut(s) 189, 560, 916
HphI GGTGA 2 cut(s) 175, 186
Hpy166II GTNNAC 4 cut(s) 194, 635, 781, 901
Hpy188I TCNGA 3 cut(s) 541, 614, 790
Hpy188III TCNNGA 7 cut(s) 28, 308, 336, 350, 437, 700, 910
Hpy8I GTNNAC 4 cut(s) 194, 635, 781, 901
Hpy99I CGWCG 1 cut(s) 755
HpyAV CCTTC 2 cut(s) 115, 262
HpyCH4IV ACGT 2 cut(s) 510, 930
HpyCH4V TGCA 4 cut(s) 8, 284, 601, 799
HpyF10VI GCNNNNNNNGC 1 cut(s) 215
HpyF3I CTNAG 1 cut(s) 147
HpySE526I ACGT 2 cut(s) 510, 930
Hsp92II CATG 7 cut(s) 104, 293, 589, 601, 650, 680, 890
HspAI GCGC 2 cut(s) 207, 759
KroI GCCGGC 1 cut(s) 915
KroNI GCCGGC 1 cut(s) 917
Ksp22I TGATCA 2 cut(s) 199, 790
KspAI GTTAAC 1 cut(s) 781
Kzo9I GATC 7 cut(s) 133, 199, 219, 589, 673, 790, 906
LmnI GCTCC 2 cut(s) 556, 766
Lsp1109I GCAGC 1 cut(s) 931
LweI GCATC 3 cut(s) 60, 271, 844
MaeI CTAG 2 cut(s) 608, 683
MaeII ACGT 2 cut(s) 510, 930
MaeIII GTNAC 3 cut(s) 40, 685, 926
MalI GATC 7 cut(s) 135, 201, 221, 591, 675, 792, 908
MboI GATC 7 cut(s) 133, 199, 219, 589, 673, 790, 906
MboII GAAGA 4 cut(s) 170, 326, 510, 766
MfeI CAATTG 1 cut(s) 794
MhlI GDGCHC 1 cut(s) 732
MluCI AATT 3 cut(s) 45, 230, 794
MmeI TCCRAC 2 cut(s) 534, 592
Mph1103I ATGCAT 1 cut(s) 286
MroNI GCCGGC 1 cut(s) 915
MseI TTAA 4 cut(s) 240, 330, 378, 780
MslI CAYNNNNRTG 2 cut(s) 602, 908
MspI CCGG 3 cut(s) 189, 560, 916
MunI CAATTG 1 cut(s) 794
MvnI CGCG 1 cut(s) 207
MwoI GCNNNNNNNGC 1 cut(s) 215
NaeI GCCGGC 1 cut(s) 917
NdeII GATC 7 cut(s) 133, 199, 219, 589, 673, 790, 906
NgoMIV GCCGGC 1 cut(s) 915
NlaIII CATG 7 cut(s) 104, 293, 589, 601, 650, 680, 890
NlaIV GGNNCC 1 cut(s) 558
NmuCI GTSAC 2 cut(s) 40, 685
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 2 cut(s) 601, 650
OliI CACNNNNGTG 1 cut(s) 908
PaeI GCATGC 1 cut(s) 601
PaeR7I CTCGAG 1 cut(s) 26
PcsI WCGNNNNNNNCGW 1 cut(s) 774
PdiI GCCGGC 1 cut(s) 917
PfeI GAWTC 2 cut(s) 68, 294
PkrI GCNGC 2 cut(s) 7, 921
Ple19I CGATCG 1 cut(s) 909
PspN4I GGNNCC 1 cut(s) 558
PspPI GGNCC 2 cut(s) 424, 890
PstNI CAGNNNCTG 1 cut(s) 65
PvuI CGATCG 1 cut(s) 909
RsaI GTAC 3 cut(s) 252, 399, 821
RsaNI GTAC 3 cut(s) 251, 398, 820
RseI CAYNNNNRTG 2 cut(s) 602, 908
SaqAI TTAA 4 cut(s) 240, 330, 378, 780
SatI GCNGC 2 cut(s) 6, 920
Sau3AI GATC 7 cut(s) 133, 199, 219, 589, 673, 790, 906
Sau96I GGNCC 2 cut(s) 424, 890
ScaI AGTACT 1 cut(s) 399
SduI GDGCHC 1 cut(s) 732
SfaNI GCATC 3 cut(s) 60, 271, 844
SfcI CTRYAG 1 cut(s) 897
Sfr274I CTCGAG 1 cut(s) 26
SinI GGWCC 2 cut(s) 424, 890
SlaI CTCGAG 1 cut(s) 26
SmiMI CAYNNNNRTG 2 cut(s) 602, 908
SmlI CTYRAG 3 cut(s) 26, 350, 419
SmoI CTYRAG 3 cut(s) 26, 350, 419
SphI GCATGC 1 cut(s) 601
Sse9I AATT 3 cut(s) 45, 230, 794
SsiI CCGC 1 cut(s) 626
SspMI CTAG 2 cut(s) 608, 683
StyI CCWWGG 1 cut(s) 652
TaiI ACGT 2 cut(s) 513, 933
TaqI TCGA 4 cut(s) 27, 753, 838, 872
TasI AATT 3 cut(s) 45, 230, 794
TatI WGTACW 2 cut(s) 397, 819
TfiI GAWTC 2 cut(s) 68, 294
Tru1I TTAA 4 cut(s) 240, 330, 378, 780
Tru9I TTAA 4 cut(s) 240, 330, 378, 780
TscAI CASTG 1 cut(s) 478
TseFI GTSAC 2 cut(s) 40, 685
TseI GCWGC 2 cut(s) 5, 919
Tsp45I GTSAC 2 cut(s) 40, 685
TspDTI ATGAA 5 cut(s) 141, 281, 327, 596, 725
TspGWI ACGGA 1 cut(s) 261
TspRI CASTG 1 cut(s) 478
VpaK11BI GGWCC 2 cut(s) 424, 890
XagI CCTNNNNNAGG 1 cut(s) 266
XapI RAATTY 1 cut(s) 230
XceI RCATGY 2 cut(s) 601, 650
XhoI CTCGAG 1 cut(s) 26
XmiI GTMKAC 1 cut(s) 900
XspI CTAG 2 cut(s) 608, 683
ZrmI AGTACT 1 cut(s) 399
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.