Rh5DG481700

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000481
Physical Location & Seq
Reverse (-)
75936219 .. 75937448
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG481700.1

Sequence Viewer

Length: 957 bp
ATGGCGCCAGCAATAGCTCAAAATGTGGAGACATCGACTGTTACTCCAACCAAAGTGACTAGCATTAAAGCACTTGCTGAGAGTTCAGCTGCTCTCAGCTCTGTACCTTCTGCATACGCCTTTACCATAAATCCCAACGACGAAGTTGATCCTAATGACCCCGAATTCGCAATTCCAATTATCGATATGTCTCTTCTCACCTGCGGTTCACCTGATCAACGCTCCAAGATCATCCAAGACCTTGTCAAAATTTGTCAAGAATGGGGATTCTTCATAGTACGCGATGAGCCGATGATCGATTCATGTCATGGATTTTTCAGTCTTCCGGAGAGTGAGAAGCAGGAGTTTAAATCAGGAAACGATGTGCTGGAGATGTTCAAGTATGGGACTAGCTATAATCTTGCATTGGACAAAGTCCTTCTCTGGAGGGACTTCTTCAAGGTCAGAACACATCCCAAATTCTACTCCCTCAACAAACCGGCAAGCTTCAGTGAGATATCATTGGAGTTCAGCAAAAGAACAAGAGAAGTAGCACTGGAAATCATCAAAGCTATATCGGAAAGCCTGGGATTGGAGCCCAACTACATATATGATGCCATGAATATGGATCGGGGCTTACAAATGCTAGCCGGGAACTACTACCCTCTTTGCCCTCAGCCAGAACATGCAATTGGTATACCACATCATACAGATCATGGTCTAGTGACACTTCTCATTCAGAATGAGATGAAGGGTCTCCAAGTCGAACACAATGGGAAATGGCTCACTGTCGATGGCCCTGCAAACGGTTTTTTTGTTAATCTTGCTGACCAAATGCAGATTCTTACTAACGGAAAGTACAAGAGTGTGATGCACCGGGCAATTGTGAATAACAAAGCTGCAAGGATATCTATAGCCATACCACATGGACCATCGATCTGTAGATACAACTATTGCACCTTCACCAGAATTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

318

Amino Acids

35.63

Weight (kDa)

5.86

Isoelectric Point (pI)

33.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 58 - 153 9.4e-15 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 207 - 301 2.5e-27 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 209
Acc36I ACCTGC 1 cut(s) 209
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 676
AccII CGCG 1 cut(s) 282
AccIII TCCGGA 1 cut(s) 325
AciI CCGC 1 cut(s) 204
AclWI GGATC 2 cut(s) 143, 615
AcsI RAATTY 3 cut(s) 164, 249, 458
AcuI CTGAAG 1 cut(s) 472
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 105, 279, 839
AfiI CCNNNNNNNGG 2 cut(s) 571, 785
AgsI TTSAA 2 cut(s) 379, 439
AjnI CCWGG 1 cut(s) 564
AjuI GAANNNNNNNTTGG 4 cut(s) 654, 686, 804, 836
AluBI AGCT 7 cut(s) 17, 89, 99, 393, 486, 551, 878
AluI AGCT 7 cut(s) 17, 89, 99, 393, 486, 551, 878
Alw26I GTCTC 3 cut(s) 23, 195, 740
AlwI GGATC 2 cut(s) 143, 615
Aor13HI TCCGGA 1 cut(s) 325
AoxI GGCC 1 cut(s) 775
ApeKI GCWGC 2 cut(s) 89, 878
ApoI RAATTY 3 cut(s) 164, 249, 458
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 776, 908
AsuC2I CCSGG 2 cut(s) 631, 857
AsuHPI GGTGA 3 cut(s) 190, 201, 934
AsuNHI GCTAGC 1 cut(s) 625
AvaII GGWCC 1 cut(s) 908
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 579
BbsI GAAGAC 1 cut(s) 314
BbvCI CCTCAGC 1 cut(s) 654
BbvI GCAGC 2 cut(s) 76, 865
BccI CCATC 2 cut(s) 767, 919
BcgI CGANNNNNNTGC 2 cut(s) 761, 795
BciT130I CCWGG 1 cut(s) 566
BclI TGATCA 1 cut(s) 214
BcnI CCSGG 2 cut(s) 631, 857
BcoDI GTCTC 3 cut(s) 23, 195, 740
BfaI CTAG 4 cut(s) 60, 390, 626, 701
BfmI CTRYAG 2 cut(s) 891, 919
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 209
BisI GCNGC 2 cut(s) 90, 879
BlsI GCNGC 2 cut(s) 91, 880
Bme1390I CCNGG 3 cut(s) 566, 631, 857
Bme18I GGWCC 1 cut(s) 908
BmgT120I GGNCC 2 cut(s) 776, 908
BmiI GGNNCC 2 cut(s) 6, 576
BmrFI CCNGG 3 cut(s) 566, 631, 857
BmsI GCATC 2 cut(s) 583, 840
BmtI GCTAGC 1 cut(s) 629
BpiI GAAGAC 1 cut(s) 314
BpmI CTGGAG 2 cut(s) 389, 445
Bpu10I CCTNAGC 1 cut(s) 654
BpuMI CCSGG 2 cut(s) 631, 857
Bsa29I ATCGAT 3 cut(s) 183, 297, 914
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 740
BsaJI CCNNGG 1 cut(s) 565
BsaWI WCCGGW 1 cut(s) 325
BsaXI ACNNNNNCTCC 2 cut(s) 28, 58
Bsc4I CCNNNNNNNGG 2 cut(s) 571, 785
Bse118I RCCGGY 1 cut(s) 478
Bse1I ACTGG 1 cut(s) 540
BseAI TCCGGA 1 cut(s) 325
BseBI CCWGG 1 cut(s) 566
BseCI ATCGAT 3 cut(s) 183, 297, 914
BseDI CCNNGG 1 cut(s) 565
BseGI GGATG 2 cut(s) 231, 451
BseLI CCNNNNNNNGG 2 cut(s) 571, 785
BseMII CTCAG 3 cut(s) 69, 109, 668
BseNI ACTGG 1 cut(s) 540
BseXI GCAGC 2 cut(s) 76, 865
Bsh1236I CGCG 1 cut(s) 282
BshFI GGCC 1 cut(s) 777
BshNI GGYRCC 1 cut(s) 4
BshVI ATCGAT 3 cut(s) 183, 297, 914
BsiSI CCGG 4 cut(s) 326, 479, 630, 856
BslFI GGGAC 2 cut(s) 400, 443
BslI CCNNNNNNNGG 2 cut(s) 571, 785
BsmAI GTCTC 3 cut(s) 23, 195, 740
BsmFI GGGAC 2 cut(s) 400, 443
BsnI GGCC 1 cut(s) 777
Bso31I GGTCTC 1 cut(s) 740
Bsp1286I GDGCHC 1 cut(s) 579
Bsp13I TCCGGA 1 cut(s) 325
Bsp143I GATC 7 cut(s) 148, 214, 228, 294, 607, 691, 915
BspACI CCGC 1 cut(s) 204
BspANI GGCC 1 cut(s) 777
BspCNI CTCAG 3 cut(s) 70, 108, 667
BspDI ATCGAT 3 cut(s) 183, 297, 914
BspEI TCCGGA 1 cut(s) 325
BspFNI CGCG 1 cut(s) 282
BspLI GGNNCC 2 cut(s) 6, 576
BspMI ACCTGC 1 cut(s) 209
BspOI GCTAGC 1 cut(s) 629
BspPI GGATC 2 cut(s) 143, 615
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 740
BsrFI RCCGGY 1 cut(s) 478
BsrI ACTGG 1 cut(s) 540
BssAI RCCGGY 1 cut(s) 478
BssECI CCNNGG 1 cut(s) 565
BssMI GATC 7 cut(s) 148, 214, 228, 294, 607, 691, 915
BssNAI GTATAC 1 cut(s) 677
BssNI GRCGYC 1 cut(s) 5
Bst1107I GTATAC 1 cut(s) 677
Bst2UI CCWGG 1 cut(s) 566
Bst4CI ACNGT 3 cut(s) 40, 769, 788
Bst6I CTCTTC 1 cut(s) 198
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 3 cut(s) 9, 484, 627
BstDEI CTNAG 3 cut(s) 78, 95, 654
BstF5I GGATG 2 cut(s) 231, 451
BstFNI CGCG 1 cut(s) 282
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 7 cut(s) 151, 217, 231, 297, 610, 694, 918
BstMAI GTCTC 3 cut(s) 23, 195, 740
BstMBI GATC 7 cut(s) 148, 214, 228, 294, 607, 691, 915
BstNI CCWGG 1 cut(s) 566
BstNSI RCATGY 1 cut(s) 668
BstSCI CCNGG 3 cut(s) 564, 629, 855
BstSFI CTRYAG 2 cut(s) 891, 919
BstUI CGCG 1 cut(s) 282
BstV1I GCAGC 2 cut(s) 76, 865
BstV2I GAAGAC 1 cut(s) 314
BstXI CCANNNNNNTGG 1 cut(s) 604
BstZ17I GTATAC 1 cut(s) 677
Bsu15I ATCGAT 3 cut(s) 183, 297, 914
BsuRI GGCC 1 cut(s) 777
BsuTUI ATCGAT 3 cut(s) 183, 297, 914
BtgZI GCGATG 1 cut(s) 297
BtsCI GGATG 2 cut(s) 231, 451
BtsIMutI CAGTG 3 cut(s) 496, 533, 765
BveI ACCTGC 1 cut(s) 209
Cac8I GCNNGC 3 cut(s) 9, 484, 627
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 478
Cfr13I GGNCC 2 cut(s) 776, 908
ClaI ATCGAT 3 cut(s) 183, 297, 914
Csp6I GTAC 3 cut(s) 104, 278, 838
CviAII CATG 6 cut(s) 303, 308, 598, 665, 695, 905
CviQI GTAC 3 cut(s) 104, 278, 838
DdeI CTNAG 3 cut(s) 78, 95, 654
DinI GGCGCC 1 cut(s) 6
DpnI GATC 7 cut(s) 150, 216, 230, 296, 609, 693, 917
DpnII GATC 7 cut(s) 148, 214, 228, 294, 607, 691, 915
DraI TTTAAA 1 cut(s) 349
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco24I GRGCYC 1 cut(s) 579
Eco31I GGTCTC 1 cut(s) 740
Eco32I GATATC 2 cut(s) 498, 888
Eco47I GGWCC 1 cut(s) 908
Eco57I CTGAAG 1 cut(s) 472
EcoRI GAATTC 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 564
EcoRV GATATC 2 cut(s) 498, 888
EcoT38I GRGCYC 1 cut(s) 579
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
FaeI CATG 6 cut(s) 306, 311, 601, 668, 698, 908
FaqI GGGAC 2 cut(s) 400, 443
FatI CATG 6 cut(s) 302, 307, 597, 664, 694, 904
FbaI TGATCA 1 cut(s) 214
FblI GTMKAC 1 cut(s) 676
Fnu4HI GCNGC 2 cut(s) 90, 879
FokI GGATG 2 cut(s) 218, 438
FriOI GRGCYC 1 cut(s) 579
Fsp4HI GCNGC 2 cut(s) 90, 879
FspBI CTAG 4 cut(s) 60, 390, 626, 701
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 90, 879
GsuI CTGGAG 2 cut(s) 389, 445
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 777
HapII CCGG 4 cut(s) 326, 479, 630, 856
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 6 cut(s) 306, 311, 601, 668, 698, 908
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HindIII AAGCTT 1 cut(s) 484
HinfI GANTC 3 cut(s) 267, 299, 820
HpaII CCGG 4 cut(s) 326, 479, 630, 856
HphI GGTGA 3 cut(s) 190, 201, 934
Hpy166II GTNNAC 2 cut(s) 209, 677
Hpy188I TCNGA 3 cut(s) 446, 559, 720
Hpy188III TCNNGA 4 cut(s) 257, 326, 354, 424
Hpy8I GTNNAC 2 cut(s) 209, 677
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 4 cut(s) 117, 428, 724, 949
HpyCH4III ACNGT 3 cut(s) 40, 769, 788
HpyCH4V TGCA 8 cut(s) 113, 404, 668, 782, 817, 853, 881, 936
HpyF3I CTNAG 3 cut(s) 78, 95, 654
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 6 cut(s) 306, 311, 601, 668, 698, 908
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Kpn2I TCCGGA 1 cut(s) 325
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 7 cut(s) 148, 214, 228, 294, 607, 691, 915
LmnI GCTCC 2 cut(s) 227, 574
Lsp1109I GCAGC 2 cut(s) 76, 865
LweI GCATC 2 cut(s) 583, 840
MaeI CTAG 4 cut(s) 60, 390, 626, 701
MaeIII GTNAC 3 cut(s) 40, 55, 703
MalI GATC 7 cut(s) 150, 216, 230, 296, 609, 693, 917
MboI GATC 7 cut(s) 148, 214, 228, 294, 607, 691, 915
MboII GAAGA 4 cut(s) 185, 262, 314, 427
MfeI CAATTG 2 cut(s) 669, 861
MhlI GDGCHC 1 cut(s) 579
MluCI AATT 8 cut(s) 164, 171, 177, 249, 458, 669, 861, 948
Mly113I GGCGCC 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 71
MnlI CCTC 4 cut(s) 420, 479, 654, 663
MroI TCCGGA 1 cut(s) 325
MseI TTAA 3 cut(s) 66, 348, 798
MslI CAYNNNNRTG 1 cut(s) 602
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 4 cut(s) 326, 479, 630, 856
MspR9I CCNGG 3 cut(s) 566, 631, 857
MunI CAATTG 2 cut(s) 669, 861
MvaI CCWGG 1 cut(s) 566
MvnI CGCG 1 cut(s) 282
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 2 cut(s) 631, 857
NdeII GATC 7 cut(s) 148, 214, 228, 294, 607, 691, 915
NheI GCTAGC 1 cut(s) 625
NlaIII CATG 6 cut(s) 306, 311, 601, 668, 698, 908
NlaIV GGNNCC 2 cut(s) 6, 576
NmuCI GTSAC 2 cut(s) 55, 703
NspI RCATGY 1 cut(s) 668
PaqCI CACCTGC 1 cut(s) 209
PfeI GAWTC 3 cut(s) 267, 299, 820
PflFI GACNNNGTC 2 cut(s) 242, 413
PkrI GCNGC 2 cut(s) 91, 880
PluTI GGCGCC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 564
PspGI CCWGG 1 cut(s) 564
PspN4I GGNNCC 2 cut(s) 6, 576
PspPI GGNCC 2 cut(s) 776, 908
PsyI GACNNNGTC 2 cut(s) 242, 413
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 3 cut(s) 105, 279, 839
RsaNI GTAC 3 cut(s) 104, 278, 838
RseI CAYNNNNRTG 1 cut(s) 602
SaqAI TTAA 3 cut(s) 66, 348, 798
SatI GCNGC 2 cut(s) 90, 879
Sau3AI GATC 7 cut(s) 148, 214, 228, 294, 607, 691, 915
Sau96I GGNCC 2 cut(s) 776, 908
ScrFI CCNGG 3 cut(s) 566, 631, 857
SduI GDGCHC 1 cut(s) 579
SfaNI GCATC 2 cut(s) 583, 840
SfcI CTRYAG 2 cut(s) 891, 919
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 908
SmiMI CAYNNNNRTG 1 cut(s) 602
Sse9I AATT 8 cut(s) 164, 171, 177, 249, 458, 669, 861, 948
SsiI CCGC 1 cut(s) 204
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 4 cut(s) 60, 390, 626, 701
StyD4I CCNGG 3 cut(s) 564, 629, 855
TaaI ACNGT 3 cut(s) 40, 769, 788
TaqI TCGA 6 cut(s) 35, 183, 297, 744, 771, 914
TasI AATT 8 cut(s) 164, 171, 177, 249, 458, 669, 861, 948
TatI WGTACW 1 cut(s) 837
TfiI GAWTC 3 cut(s) 267, 299, 820
Tru1I TTAA 3 cut(s) 66, 348, 798
Tru9I TTAA 3 cut(s) 66, 348, 798
TscAI CASTG 3 cut(s) 496, 540, 772
TseFI GTSAC 2 cut(s) 55, 703
TseI GCWGC 2 cut(s) 89, 878
Tsp45I GTSAC 2 cut(s) 55, 703
TspDTI ATGAA 4 cut(s) 262, 291, 614, 743
TspGWI ACGGA 1 cut(s) 846
TspRI CASTG 3 cut(s) 496, 540, 772
Tth111I GACNNNGTC 2 cut(s) 242, 413
VpaK11BI GGWCC 1 cut(s) 908
XapI RAATTY 3 cut(s) 164, 249, 458
XceI RCATGY 1 cut(s) 668
XmiI GTMKAC 1 cut(s) 676
XspI CTAG 4 cut(s) 60, 390, 626, 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.