Rroxscaffold_3G00252020

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000481
Physical Location & Seq
Forward (+)
46004049 .. 46005887
1839 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00252020.1

Sequence Viewer

Length: 1068 bp
ATGGCTGCAGTTGCTCAAGCCCAGGAGACATCAAATGTCACAAGCATCAAATCGCTAGCCGAGTCACCAGCTCTCAACTCTGTGCCTTCTGCATATGCCTTCAACATAAACCCCAATGATGAAGCTGATCCTAATGACCCTGAGTTTGCTATTCCCATTGTTGATATGTCTCTTCTCACCTCTGGTTCTCCCGACGAGCGCGCAAAAATCGTTGGCGATCTTGTCAAAATTTGTGAAGAATGGGGCTTCTTCATTGCAATTAACCATGGAGTACCAGAGAGCCTAATGAAGGCGATGATTGACGCATGTCATGGATTTTTCAGTCTTCCAGACGAGGAGAAGAAGGAGTTTAAATCCGGAAATGATGTTCTCGAGATGTTCAAGTATGGAACAAGCTATAATCTAGCACTGGACAAAGTACTTTTATGGAGGGACTTCTTCAAGGTCCGAACACACCCGGAGTTTCACTCCCTCTACAAACCAGCTAGCTTCAGTGAGATTTCGCTGGAGTTTAGCAAAAGAAGCCGAGAAGTAGCCTTGGAAATAACGAGAGCAATATCCGAAAGCTTGGGTTTGGGGCCGGACTACATATACAACACAATGAACATGGATCGCGGCTTACAAATGCTAGCGGGGAACTACTACCCACCTTGTCCTCAGCCTGAACATGCCATTGGTATACCCCATCATACCGATCATGGTCTAGTCACACTCCTCATTCAGAATGAGATGAATGGTCTCCAAGTTGAGCACAATGGAAAATGGCTCACTGTCAATGGCCCTCCTAATGGCTTTTTCGTCAACCTTGCTGATCAAATGCAGATTCTTACAAATGGTAAGTACAAGAGTGTGATGCATCGGGCCACAGTGAACAACAAAGCTACAAGGATATCGATAGCTATACCACATGGACCATCCGTAGACACGATCATAGCACCAGCAGCAGAGTTGTGTGAAAGAGAAGGCCAAGCTCCAAAGTACCTTGCTATGAACTACAAGGAATACATACAACTTCAGCAAAGCGGCAAGAACTACATGAAGTCCACATTTGATCATATCCGAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

355

Amino Acids

39.6

Weight (kDa)

5.56

Isoelectric Point (pI)

34.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 51 - 161 1.9e-25 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 208 - 302 7.2e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 679, 921
AccII CGCG 2 cut(s) 201, 615
AccIII TCCGGA 1 cut(s) 356
AciI CCGC 3 cut(s) 615, 632, 1023
AclWI GGATC 2 cut(s) 122, 618
AcsI RAATTY 1 cut(s) 228
AcuI CTGAAG 2 cut(s) 475, 998
AfaI GTAC 4 cut(s) 273, 420, 842, 980
AfiI CCNNNNNNNGG 2 cut(s) 289, 788
AgsI TTSAA 3 cut(s) 103, 382, 442
AjnI CCWGG 1 cut(s) 21
AjuI GAANNNNNNNTTGG 2 cut(s) 657, 689
AloI GAACNNNNNNTCC 2 cut(s) 351, 383
AluBI AGCT 9 cut(s) 71, 125, 396, 485, 489, 567, 881, 899, 971
AluI AGCT 9 cut(s) 71, 125, 396, 485, 489, 567, 881, 899, 971
Alw21I GWGCWC 1 cut(s) 753
Alw26I GTCTC 3 cut(s) 20, 174, 743
AlwI GGATC 2 cut(s) 122, 618
Ama87I CYCGRG 1 cut(s) 371
Aor13HI TCCGGA 1 cut(s) 356
AoxI GGCC 4 cut(s) 578, 778, 861, 964
ApeKI GCWGC 2 cut(s) 5, 941
ApoI RAATTY 1 cut(s) 228
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AspLEI GCGC 2 cut(s) 201, 203
AspS9I GGNCC 5 cut(s) 445, 578, 779, 861, 911
AsuC2I CCSGG 1 cut(s) 458
AsuHPI GGTGA 2 cut(s) 57, 169
AsuNHI GCTAGC 3 cut(s) 55, 485, 628
AvaI CYCGRG 1 cut(s) 371
AvaII GGWCC 2 cut(s) 445, 911
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 1 cut(s) 753
BbvCI CCTCAGC 1 cut(s) 657
BbvI GCAGC 1 cut(s) 953
BccI CCATC 2 cut(s) 693, 922
BciT130I CCWGG 1 cut(s) 23
BclI TGATCA 2 cut(s) 811, 1051
BcnI CCSGG 1 cut(s) 458
BcoDI GTCTC 3 cut(s) 20, 174, 743
BfaI CTAG 6 cut(s) 56, 404, 486, 629, 704, 1066
BfmI CTRYAG 1 cut(s) 6
BisI GCNGC 4 cut(s) 6, 616, 942, 1024
BlsI GCNGC 4 cut(s) 7, 617, 943, 1025
BmcAI AGTACT 1 cut(s) 420
Bme1390I CCNGG 2 cut(s) 23, 458
Bme18I GGWCC 2 cut(s) 445, 911
BmeT110I CYCGRG 1 cut(s) 371
BmgT120I GGNCC 5 cut(s) 445, 578, 779, 861, 911
BmiI GGNNCC 1 cut(s) 579
BmrFI CCNGG 2 cut(s) 23, 458
BmsI GCATC 3 cut(s) 54, 843, 865
BmtI GCTAGC 3 cut(s) 59, 489, 632
BoxI GACNNNNGTC 1 cut(s) 306
BpiI GAAGAC 1 cut(s) 317
BplI GAGNNNNNCTC 2 cut(s) 452, 484
BpmI CTGGAG 1 cut(s) 527
Bpu10I CCTNAGC 1 cut(s) 657
BpuMI CCSGG 1 cut(s) 458
Bsa29I ATCGAT 1 cut(s) 893
BsaI GGTCTC 1 cut(s) 743
BsaJI CCNNGG 3 cut(s) 21, 265, 537
BsaWI WCCGGW 1 cut(s) 356
Bsc4I CCNNNNNNNGG 2 cut(s) 289, 788
Bse1I ACTGG 1 cut(s) 414
Bse3DI GCAATG 1 cut(s) 252
BseAI TCCGGA 1 cut(s) 356
BseBI CCWGG 1 cut(s) 23
BseCI ATCGAT 1 cut(s) 893
BseDI CCNNGG 3 cut(s) 21, 265, 537
BseGI GGATG 1 cut(s) 914
BseLI CCNNNNNNNGG 2 cut(s) 289, 788
BseMI GCAATG 1 cut(s) 252
BseMII CTCAG 2 cut(s) 132, 671
BseNI ACTGG 1 cut(s) 414
BsePI GCGCGC 1 cut(s) 199
BseRI GAGGAG 2 cut(s) 350, 704
BseXI GCAGC 1 cut(s) 953
Bsh1236I CGCG 2 cut(s) 201, 615
BshFI GGCC 4 cut(s) 580, 780, 863, 966
BshVI ATCGAT 1 cut(s) 893
BsiHKAI GWGCWC 1 cut(s) 753
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 3 cut(s) 357, 458, 581
BslFI GGGAC 1 cut(s) 446
BslI CCNNNNNNNGG 2 cut(s) 289, 788
BsmAI GTCTC 3 cut(s) 20, 174, 743
BsmFI GGGAC 1 cut(s) 446
BsnI GGCC 4 cut(s) 580, 780, 863, 966
Bso31I GGTCTC 1 cut(s) 743
BsoBI CYCGRG 1 cut(s) 371
Bsp1286I GDGCHC 1 cut(s) 753
Bsp13I TCCGGA 1 cut(s) 356
Bsp143I GATC 7 cut(s) 127, 217, 610, 694, 811, 927, 1051
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 3 cut(s) 615, 632, 1023
BspANI GGCC 4 cut(s) 580, 780, 863, 966
BspCNI CTCAG 2 cut(s) 133, 670
BspDI ATCGAT 1 cut(s) 893
BspEI TCCGGA 1 cut(s) 356
BspFNI CGCG 2 cut(s) 201, 615
BspLI GGNNCC 1 cut(s) 579
BspMAI CTGCAG 1 cut(s) 10
BspOI GCTAGC 3 cut(s) 59, 489, 632
BspPI GGATC 2 cut(s) 122, 618
BspTNI GGTCTC 1 cut(s) 743
BsrDI GCAATG 1 cut(s) 252
BsrI ACTGG 1 cut(s) 414
BssECI CCNNGG 3 cut(s) 21, 265, 537
BssHII GCGCGC 1 cut(s) 199
BssMI GATC 7 cut(s) 127, 217, 610, 694, 811, 927, 1051
BssNAI GTATAC 1 cut(s) 680
BssT1I CCWWGG 2 cut(s) 265, 537
Bst1107I GTATAC 1 cut(s) 680
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 2 cut(s) 772, 868
Bst6I CTCTTC 1 cut(s) 177
BstC8I GCNNGC 4 cut(s) 57, 201, 487, 630
BstDEI CTNAG 2 cut(s) 141, 657
BstDSI CCRYGG 1 cut(s) 265
BstENI CCTNNNNNAGG 1 cut(s) 287
BstF5I GGATG 1 cut(s) 914
BstFNI CGCG 2 cut(s) 201, 615
BstHHI GCGC 2 cut(s) 201, 203
BstKTI GATC 7 cut(s) 130, 220, 613, 697, 814, 930, 1054
BstMAI GTCTC 3 cut(s) 20, 174, 743
BstMBI GATC 7 cut(s) 127, 217, 610, 694, 811, 927, 1051
BstMWI GCNNNNNNNGC 3 cut(s) 11, 522, 941
BstNI CCWGG 1 cut(s) 23
BstNSI RCATGY 2 cut(s) 309, 671
BstPAI GACNNNNGTC 1 cut(s) 306
BstSCI CCNGG 2 cut(s) 21, 456
BstSFI CTRYAG 1 cut(s) 6
BstUI CGCG 2 cut(s) 201, 615
BstV1I GCAGC 1 cut(s) 953
BstV2I GAAGAC 1 cut(s) 317
BstZ17I GTATAC 1 cut(s) 680
Bsu15I ATCGAT 1 cut(s) 893
BsuRI GGCC 4 cut(s) 580, 780, 863, 966
BsuTUI ATCGAT 1 cut(s) 893
BtgI CCRYGG 1 cut(s) 265
BtgZI GCGATG 1 cut(s) 308
BtsCI GGATG 1 cut(s) 914
BtsIMutI CAGTG 4 cut(s) 407, 499, 768, 873
Cac8I GCNNGC 4 cut(s) 57, 201, 487, 630
CfoI GCGC 2 cut(s) 201, 203
Cfr13I GGNCC 5 cut(s) 445, 578, 779, 861, 911
ClaI ATCGAT 1 cut(s) 893
CseI GACGC 1 cut(s) 311
Csp6I GTAC 4 cut(s) 272, 419, 841, 979
CviAII CATG 8 cut(s) 266, 306, 311, 607, 668, 698, 908, 1036
CviQI GTAC 4 cut(s) 272, 419, 841, 979
DdeI CTNAG 2 cut(s) 141, 657
DpnI GATC 7 cut(s) 129, 219, 612, 696, 813, 929, 1053
DpnII GATC 7 cut(s) 127, 217, 610, 694, 811, 927, 1051
DraI TTTAAA 1 cut(s) 352
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Eco130I CCWWGG 2 cut(s) 265, 537
Eco31I GGTCTC 1 cut(s) 743
Eco32I GATATC 1 cut(s) 891
Eco47I GGWCC 2 cut(s) 445, 911
Eco57I CTGAAG 2 cut(s) 475, 998
Eco88I CYCGRG 1 cut(s) 371
EcoNI CCTNNNNNAGG 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 21
EcoRV GATATC 1 cut(s) 891
EcoT14I CCWWGG 2 cut(s) 265, 537
EcoT22I ATGCAT 1 cut(s) 858
ErhI CCWWGG 2 cut(s) 265, 537
FaeI CATG 8 cut(s) 269, 309, 314, 610, 671, 701, 911, 1039
FaqI GGGAC 1 cut(s) 446
FatI CATG 8 cut(s) 265, 305, 310, 606, 667, 697, 907, 1035
FauI CCCGC 1 cut(s) 625
FauNDI CATATG 1 cut(s) 94
FbaI TGATCA 2 cut(s) 811, 1051
FblI GTMKAC 2 cut(s) 679, 921
Fnu4HI GCNGC 4 cut(s) 6, 616, 942, 1024
FokI GGATG 1 cut(s) 901
Fsp4HI GCNGC 4 cut(s) 6, 616, 942, 1024
FspBI CTAG 6 cut(s) 56, 404, 486, 629, 704, 1066
GlaI GCGC 2 cut(s) 200, 202
GluI GCNGC 4 cut(s) 6, 616, 942, 1024
GsuI CTGGAG 1 cut(s) 527
HaeIII GGCC 4 cut(s) 580, 780, 863, 966
HapII CCGG 3 cut(s) 357, 458, 581
HgaI GACGC 1 cut(s) 311
HhaI GCGC 2 cut(s) 201, 203
Hin1II CATG 8 cut(s) 269, 309, 314, 610, 671, 701, 911, 1039
Hin6I GCGC 2 cut(s) 199, 201
HinP1I GCGC 2 cut(s) 199, 201
HincII GTYRAC 1 cut(s) 802
HindII GTYRAC 1 cut(s) 802
HindIII AAGCTT 1 cut(s) 565
HinfI GANTC 2 cut(s) 62, 823
HpaII CCGG 3 cut(s) 357, 458, 581
HphI GGTGA 2 cut(s) 57, 169
Hpy166II GTNNAC 5 cut(s) 680, 802, 871, 922, 1044
Hpy188I TCNGA 4 cut(s) 449, 562, 723, 1061
Hpy188III TCNNGA 5 cut(s) 191, 329, 357, 371, 373
Hpy8I GTNNAC 5 cut(s) 680, 802, 871, 922, 1044
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 5 cut(s) 96, 109, 283, 337, 956
HpyCH4III ACNGT 2 cut(s) 772, 868
HpyCH4V TGCA 5 cut(s) 8, 92, 257, 820, 856
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 522, 941
HpyF3I CTNAG 2 cut(s) 141, 657
Hsp92II CATG 8 cut(s) 269, 309, 314, 610, 671, 701, 911, 1039
HspAI GCGC 2 cut(s) 199, 201
Kpn2I TCCGGA 1 cut(s) 356
Ksp22I TGATCA 2 cut(s) 811, 1051
Kzo9I GATC 7 cut(s) 127, 217, 610, 694, 811, 927, 1051
LmnI GCTCC 1 cut(s) 976
Lsp1109I GCAGC 1 cut(s) 953
LweI GCATC 3 cut(s) 54, 843, 865
MaeI CTAG 6 cut(s) 56, 404, 486, 629, 704, 1066
MaeIII GTNAC 3 cut(s) 37, 63, 706
MalI GATC 7 cut(s) 129, 219, 612, 696, 813, 929, 1053
MboI GATC 7 cut(s) 127, 217, 610, 694, 811, 927, 1051
MboII GAAGA 6 cut(s) 164, 241, 248, 317, 352, 430
MhlI GDGCHC 1 cut(s) 753
MluCI AATT 2 cut(s) 228, 258
MlyI GAGTC 1 cut(s) 71
MnlI CCTC 7 cut(s) 190, 328, 423, 482, 666, 725, 792
Mph1103I ATGCAT 1 cut(s) 858
MroI TCCGGA 1 cut(s) 356
MseI TTAA 2 cut(s) 261, 351
MspI CCGG 3 cut(s) 357, 458, 581
MspR9I CCNGG 2 cut(s) 23, 458
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 2 cut(s) 201, 615
MwoI GCNNNNNNNGC 3 cut(s) 11, 522, 941
NciI CCSGG 1 cut(s) 458
NcoI CCATGG 1 cut(s) 265
NdeI CATATG 1 cut(s) 94
NdeII GATC 7 cut(s) 127, 217, 610, 694, 811, 927, 1051
NheI GCTAGC 3 cut(s) 55, 485, 628
NlaIII CATG 8 cut(s) 269, 309, 314, 610, 671, 701, 911, 1039
NlaIV GGNNCC 1 cut(s) 579
NmeAIII GCCGAG 2 cut(s) 85, 551
NmuCI GTSAC 3 cut(s) 37, 63, 706
NsiI ATGCAT 1 cut(s) 858
NspI RCATGY 2 cut(s) 309, 671
PaeR7I CTCGAG 1 cut(s) 371
PauI GCGCGC 1 cut(s) 199
PfeI GAWTC 1 cut(s) 823
PkrI GCNGC 4 cut(s) 7, 617, 943, 1025
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PshAI GACNNNNGTC 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 1 cut(s) 579
PspPI GGNCC 5 cut(s) 445, 578, 779, 861, 911
PstI CTGCAG 1 cut(s) 10
PteI GCGCGC 1 cut(s) 199
RsaI GTAC 4 cut(s) 273, 420, 842, 980
RsaNI GTAC 4 cut(s) 272, 419, 841, 979
SaqAI TTAA 2 cut(s) 261, 351
SatI GCNGC 4 cut(s) 6, 616, 942, 1024
Sau3AI GATC 7 cut(s) 127, 217, 610, 694, 811, 927, 1051
Sau96I GGNCC 5 cut(s) 445, 578, 779, 861, 911
ScaI AGTACT 1 cut(s) 420
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 2 cut(s) 23, 458
SduI GDGCHC 1 cut(s) 753
SfaNI GCATC 3 cut(s) 54, 843, 865
SfcI CTRYAG 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 371
SinI GGWCC 2 cut(s) 445, 911
SlaI CTCGAG 1 cut(s) 371
SmlI CTYRAG 2 cut(s) 15, 371
SmoI CTYRAG 2 cut(s) 15, 371
Sse9I AATT 2 cut(s) 228, 258
SsiI CCGC 3 cut(s) 615, 632, 1023
SspMI CTAG 6 cut(s) 56, 404, 486, 629, 704, 1066
StyD4I CCNGG 2 cut(s) 21, 456
StyI CCWWGG 2 cut(s) 265, 537
TaaI ACNGT 2 cut(s) 772, 868
TaqI TCGA 2 cut(s) 372, 893
TasI AATT 2 cut(s) 228, 258
TatI WGTACW 2 cut(s) 418, 840
TauI GCSGC 2 cut(s) 618, 1026
TfiI GAWTC 1 cut(s) 823
Tru1I TTAA 2 cut(s) 261, 351
Tru9I TTAA 2 cut(s) 261, 351
TscAI CASTG 4 cut(s) 414, 499, 775, 873
TseFI GTSAC 3 cut(s) 37, 63, 706
TseI GCWGC 2 cut(s) 5, 941
Tsp45I GTSAC 3 cut(s) 37, 63, 706
TspDTI ATGAA 7 cut(s) 135, 241, 302, 617, 746, 1004, 1052
TspGWI ACGGA 1 cut(s) 907
TspRI CASTG 4 cut(s) 414, 499, 775, 873
VpaK11BI GGWCC 2 cut(s) 445, 911
XagI CCTNNNNNAGG 1 cut(s) 287
XapI RAATTY 1 cut(s) 228
XceI RCATGY 2 cut(s) 309, 671
XhoI CTCGAG 1 cut(s) 371
XmiI GTMKAC 2 cut(s) 679, 921
XspI CTAG 6 cut(s) 56, 404, 486, 629, 704, 1066
ZrmI AGTACT 1 cut(s) 420
Zsp2I ATGCAT 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.