FvH4_5g20421

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000481
Physical Location & Seq
Reverse (-)
12077921 .. 12079814
1894 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20421.t1

Sequence Viewer

Length: 1098 bp
ATGGCTGCAGTTTCTCAAGCCTTGGAAACCTCAAATGTCACAAGCATCAAATCACTAGCTGAGTCACCAGCTCTCAGCTCTGTCCCTTCTGCTTATGCCTTCAACATTAACCCCAATGAGGAAGCTGATCCTAATGACCCTGAGTTTGCCATTCCCATTGTTGATATGTCTCTTCTCACCTCTGGTTCTCCTGATGAGCGCGCGAAGATCATCCGCGATCTTGTCAAAATCTGTCAAGAATGGGGCTTCTTCATCGCAGTTAACCACGGAGTACCAGAGAGCCTGATGAAGGAGATGATTGACGCATGCCATGGATTTTTCAGTCTTCCGGATGAGGAGAAGAAGGAGTTTAAGTCAGGAAATGATGTTCTCGAGATGTTCAAGTGTGGAACAAGCTATAATCTAGCATTGGACAAAGTACTTTTATGGAGGGACTTCTTCAAGGTTCGAACACATCCTGAGTTTCACTCTCTCAACAAACCAGCTAGCTTCAGTGTGGTTTCGCTGGAGTTCAGCAGAAGAAGCCGAGAAGTAGCACTGGAAATAACAAGAGCAATATCAGAAAGCTTGGGGTTGGAGCCGGATTACATATATAACAAAATGAACATGGATCGCGGCATACAAATGCTAGCAGGGAACTACTACCCACCTTGCCCTGAGCCTGAGCATGCCATTGGTATACCCCATCATACCGATCATGGTCTAGTCACTCTCCTCATCCAAAATGAAATGAATGGCCTCCAAGTCGAGCACAATGGAAAATGGCTCACTGTCAATGGCCCTAAGAATGGCTTTTTTGTTAACCTTGCAGACCAAATGCAGATTCTTACAAACGGTAAGTACAAGAGTGTGATGCATCGCGCCACCGTGAACAACAAAGCTACGAGGATATCGATAGCCATACCACATGGACCATCCGTAGACACGGTCATAGCACCGGCACCTGAGTTGTGTGAAAGAGAAGGCAAAGCTCCAAAGTACCTTGCTATGAACTACAAGGAATACATACAACTTCAGCAAGGCGGCAAGAACTACAAGTTCTTTTTGAACAACGGCAAGAACTACATGAAGTCCACATTTGATCACATCCTAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

366

Amino Acids

40.89

Weight (kDa)

5.97

Isoelectric Point (pI)

36.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 51 - 158 3.7e-25 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 208 - 302 2.3e-29 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 940
AccI GTMKAC 2 cut(s) 679, 921
AccII CGCG 5 cut(s) 201, 203, 216, 615, 861
AccIII TCCGGA 1 cut(s) 328
AciI CCGC 3 cut(s) 214, 615, 1023
AclWI GGATC 2 cut(s) 122, 618
AcuI CTGAAG 2 cut(s) 475, 998
AfaI GTAC 4 cut(s) 273, 420, 842, 980
AfiI CCNNNNNNNGG 3 cut(s) 118, 289, 788
AgsI TTSAA 4 cut(s) 103, 382, 442, 1048
AjuI GAANNNNNNNTTGG 2 cut(s) 807, 839
AloI GAACNNNNNNTCC 2 cut(s) 351, 383
Alw21I GWGCWC 1 cut(s) 753
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 2 cut(s) 122, 618
Ama87I CYCGRG 1 cut(s) 371
Aor13HI TCCGGA 1 cut(s) 328
AoxI GGCC 2 cut(s) 736, 778
ApeKI GCWGC 1 cut(s) 5
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AspLEI GCGC 3 cut(s) 201, 203, 863
AspS9I GGNCC 2 cut(s) 779, 911
AsuHPI GGTGA 2 cut(s) 57, 169
AsuII TTCGAA 1 cut(s) 448
AsuNHI GCTAGC 2 cut(s) 485, 628
AvaI CYCGRG 1 cut(s) 371
AvaII GGWCC 1 cut(s) 911
BanI GGYRCC 1 cut(s) 940
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 1 cut(s) 753
BccI CCATC 2 cut(s) 693, 922
BceAI ACGGC 1 cut(s) 1069
BclI TGATCA 1 cut(s) 1081
BcoDI GTCTC 1 cut(s) 174
BfaI CTAG 7 cut(s) 56, 404, 486, 629, 704, 1091, 1096
BfmI CTRYAG 1 cut(s) 6
BisI GCNGC 3 cut(s) 6, 616, 1024
BlsI GCNGC 3 cut(s) 7, 617, 1025
BmcAI AGTACT 1 cut(s) 420
Bme18I GGWCC 1 cut(s) 911
BmeT110I CYCGRG 1 cut(s) 371
BmgT120I GGNCC 2 cut(s) 779, 911
BmiI GGNNCC 2 cut(s) 579, 942
BmsI GCATC 3 cut(s) 54, 843, 865
BmtI GCTAGC 2 cut(s) 489, 632
BpiI GAAGAC 1 cut(s) 317
BplI GAGNNNNNCTC 2 cut(s) 452, 484
BpmI CTGGAG 1 cut(s) 527
Bpu10I CCTNAGC 2 cut(s) 657, 663
Bpu14I TTCGAA 1 cut(s) 448
Bsa29I ATCGAT 1 cut(s) 893
BsaJI CCNNGG 3 cut(s) 21, 265, 310
BsaWI WCCGGW 1 cut(s) 328
BsaXI ACNNNNNCTCC 2 cut(s) 338, 368
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 289, 788
Bse118I RCCGGY 1 cut(s) 937
Bse1I ACTGG 1 cut(s) 543
BseAI TCCGGA 1 cut(s) 328
BseCI ATCGAT 1 cut(s) 893
BseDI CCNNGG 3 cut(s) 21, 265, 310
BseGI GGATG 6 cut(s) 210, 337, 454, 717, 914, 1086
BseLI CCNNNNNNNGG 3 cut(s) 118, 289, 788
BseMII CTCAG 7 cut(s) 51, 88, 132, 450, 648, 654, 936
BseNI ACTGG 1 cut(s) 543
BsePI GCGCGC 1 cut(s) 199
BseRI GAGGAG 2 cut(s) 350, 704
Bsh1236I CGCG 5 cut(s) 201, 203, 216, 615, 861
BshFI GGCC 2 cut(s) 738, 780
BshNI GGYRCC 1 cut(s) 940
BshVI ATCGAT 1 cut(s) 893
BsiHKAI GWGCWC 1 cut(s) 753
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 3 cut(s) 329, 581, 938
BslFI GGGAC 2 cut(s) 68, 446
BslI CCNNNNNNNGG 3 cut(s) 118, 289, 788
BsmAI GTCTC 1 cut(s) 174
BsmFI GGGAC 2 cut(s) 68, 446
BsnI GGCC 2 cut(s) 738, 780
BsoBI CYCGRG 1 cut(s) 371
Bsp119I TTCGAA 1 cut(s) 448
Bsp1286I GDGCHC 1 cut(s) 753
Bsp13I TCCGGA 1 cut(s) 328
Bsp143I GATC 6 cut(s) 127, 207, 217, 610, 694, 1081
Bsp19I CCATGG 1 cut(s) 310
BspACI CCGC 3 cut(s) 214, 615, 1023
BspANI GGCC 2 cut(s) 738, 780
BspCNI CTCAG 7 cut(s) 52, 87, 133, 451, 649, 655, 937
BspDI ATCGAT 1 cut(s) 893
BspEI TCCGGA 1 cut(s) 328
BspFNI CGCG 5 cut(s) 201, 203, 216, 615, 861
BspLI GGNNCC 2 cut(s) 579, 942
BspMAI CTGCAG 1 cut(s) 10
BspOI GCTAGC 2 cut(s) 489, 632
BspPI GGATC 2 cut(s) 122, 618
BspT104I TTCGAA 1 cut(s) 448
BspT107I GGYRCC 1 cut(s) 940
BsrFI RCCGGY 1 cut(s) 937
BsrI ACTGG 1 cut(s) 543
BssAI RCCGGY 1 cut(s) 937
BssECI CCNNGG 3 cut(s) 21, 265, 310
BssHII GCGCGC 1 cut(s) 199
BssMI GATC 6 cut(s) 127, 207, 217, 610, 694, 1081
BssNAI GTATAC 1 cut(s) 680
BssT1I CCWWGG 2 cut(s) 21, 310
Bst1107I GTATAC 1 cut(s) 680
Bst4CI ACNGT 4 cut(s) 772, 836, 868, 928
Bst6I CTCTTC 1 cut(s) 177
BstBI TTCGAA 1 cut(s) 448
BstC8I GCNNGC 5 cut(s) 201, 307, 487, 630, 669
BstDEI CTNAG 8 cut(s) 60, 74, 141, 459, 657, 663, 783, 945
BstDSI CCRYGG 2 cut(s) 265, 310
BstENI CCTNNNNNAGG 1 cut(s) 287
BstF5I GGATG 6 cut(s) 210, 337, 454, 717, 914, 1086
BstFNI CGCG 5 cut(s) 201, 203, 216, 615, 861
BstHHI GCGC 3 cut(s) 201, 203, 863
BstKTI GATC 6 cut(s) 130, 210, 220, 613, 697, 1084
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 6 cut(s) 127, 207, 217, 610, 694, 1081
BstMWI GCNNNNNNNGC 1 cut(s) 522
BstNSI RCATGY 2 cut(s) 309, 671
BstSFI CTRYAG 1 cut(s) 6
BstUI CGCG 5 cut(s) 201, 203, 216, 615, 861
BstV2I GAAGAC 1 cut(s) 317
BstZ17I GTATAC 1 cut(s) 680
Bsu15I ATCGAT 1 cut(s) 893
BsuRI GGCC 2 cut(s) 738, 780
BsuTUI ATCGAT 1 cut(s) 893
BtgI CCRYGG 2 cut(s) 265, 310
BtgZI GCGATG 2 cut(s) 238, 842
BtsCI GGATG 6 cut(s) 210, 337, 454, 717, 914, 1086
BtsIMutI CAGTG 3 cut(s) 499, 536, 768
Cac8I GCNNGC 5 cut(s) 201, 307, 487, 630, 669
CfoI GCGC 3 cut(s) 201, 203, 863
Cfr10I RCCGGY 1 cut(s) 937
Cfr13I GGNCC 2 cut(s) 779, 911
ClaI ATCGAT 1 cut(s) 893
CseI GACGC 1 cut(s) 311
Csp6I GTAC 4 cut(s) 272, 419, 841, 979
CviAII CATG 7 cut(s) 306, 311, 607, 668, 698, 908, 1066
CviQI GTAC 4 cut(s) 272, 419, 841, 979
DdeI CTNAG 8 cut(s) 60, 74, 141, 459, 657, 663, 783, 945
DpnI GATC 6 cut(s) 129, 209, 219, 612, 696, 1083
DpnII GATC 6 cut(s) 127, 207, 217, 610, 694, 1081
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Eco130I CCWWGG 2 cut(s) 21, 310
Eco32I GATATC 1 cut(s) 891
Eco47I GGWCC 1 cut(s) 911
Eco57I CTGAAG 2 cut(s) 475, 998
Eco88I CYCGRG 1 cut(s) 371
EcoNI CCTNNNNNAGG 1 cut(s) 287
EcoRV GATATC 1 cut(s) 891
EcoT14I CCWWGG 2 cut(s) 21, 310
EcoT22I ATGCAT 1 cut(s) 858
ErhI CCWWGG 2 cut(s) 21, 310
FaeI CATG 7 cut(s) 309, 314, 610, 671, 701, 911, 1069
FalI AAGNNNNNCTT 2 cut(s) 776, 808
FaqI GGGAC 2 cut(s) 68, 446
FatI CATG 7 cut(s) 305, 310, 606, 667, 697, 907, 1065
FbaI TGATCA 1 cut(s) 1081
FblI GTMKAC 2 cut(s) 679, 921
Fnu4HI GCNGC 3 cut(s) 6, 616, 1024
FokI GGATG 6 cut(s) 197, 344, 441, 704, 901, 1073
Fsp4HI GCNGC 3 cut(s) 6, 616, 1024
FspBI CTAG 7 cut(s) 56, 404, 486, 629, 704, 1091, 1096
GlaI GCGC 3 cut(s) 200, 202, 862
GluI GCNGC 3 cut(s) 6, 616, 1024
GsuI CTGGAG 1 cut(s) 527
HaeIII GGCC 2 cut(s) 738, 780
HapII CCGG 3 cut(s) 329, 581, 938
HgaI GACGC 1 cut(s) 311
HhaI GCGC 3 cut(s) 201, 203, 863
Hin1II CATG 7 cut(s) 309, 314, 610, 671, 701, 911, 1069
Hin6I GCGC 3 cut(s) 199, 201, 861
HinP1I GCGC 3 cut(s) 199, 201, 861
HincII GTYRAC 2 cut(s) 262, 802
HindII GTYRAC 2 cut(s) 262, 802
HindIII AAGCTT 1 cut(s) 565
HinfI GANTC 2 cut(s) 62, 823
HpaI GTTAAC 2 cut(s) 262, 802
HpaII CCGG 3 cut(s) 329, 581, 938
HphI GGTGA 2 cut(s) 57, 169
Hpy166II GTNNAC 6 cut(s) 262, 680, 802, 871, 922, 1074
Hpy188I TCNGA 1 cut(s) 562
Hpy188III TCNNGA 7 cut(s) 191, 236, 329, 357, 371, 373, 458
Hpy8I GTNNAC 6 cut(s) 262, 680, 802, 871, 922, 1074
HpyAV CCTTC 5 cut(s) 96, 109, 283, 337, 956
HpyCH4III ACNGT 4 cut(s) 772, 836, 868, 928
HpyCH4V TGCA 4 cut(s) 8, 809, 820, 856
HpyF10VI GCNNNNNNNGC 1 cut(s) 522
HpyF3I CTNAG 8 cut(s) 60, 74, 141, 459, 657, 663, 783, 945
Hsp92II CATG 7 cut(s) 309, 314, 610, 671, 701, 911, 1069
HspAI GCGC 3 cut(s) 199, 201, 861
Kpn2I TCCGGA 1 cut(s) 328
Ksp22I TGATCA 1 cut(s) 1081
KspAI GTTAAC 2 cut(s) 262, 802
Kzo9I GATC 6 cut(s) 127, 207, 217, 610, 694, 1081
LmnI GCTCC 2 cut(s) 577, 976
LweI GCATC 3 cut(s) 54, 843, 865
MaeI CTAG 7 cut(s) 56, 404, 486, 629, 704, 1091, 1096
MaeIII GTNAC 3 cut(s) 37, 63, 706
MalI GATC 6 cut(s) 129, 209, 219, 612, 696, 1083
MboI GATC 6 cut(s) 127, 207, 217, 610, 694, 1081
MboII GAAGA 7 cut(s) 164, 217, 241, 317, 352, 430, 531
MhlI GDGCHC 1 cut(s) 753
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 1 cut(s) 555
MnlI CCTC 8 cut(s) 40, 112, 190, 328, 423, 725, 749, 879
Mph1103I ATGCAT 1 cut(s) 858
MroI TCCGGA 1 cut(s) 328
MseI TTAA 4 cut(s) 108, 261, 351, 801
MslI CAYNNNNRTG 1 cut(s) 623
MspI CCGG 3 cut(s) 329, 581, 938
MvnI CGCG 5 cut(s) 201, 203, 216, 615, 861
MwoI GCNNNNNNNGC 1 cut(s) 522
NcoI CCATGG 1 cut(s) 310
NdeII GATC 6 cut(s) 127, 207, 217, 610, 694, 1081
NheI GCTAGC 2 cut(s) 485, 628
NlaIII CATG 7 cut(s) 309, 314, 610, 671, 701, 911, 1069
NlaIV GGNNCC 2 cut(s) 579, 942
NmeAIII GCCGAG 1 cut(s) 551
NmuCI GTSAC 3 cut(s) 37, 63, 706
NsiI ATGCAT 1 cut(s) 858
NspI RCATGY 2 cut(s) 309, 671
NspV TTCGAA 1 cut(s) 448
PaeI GCATGC 2 cut(s) 309, 671
PaeR7I CTCGAG 1 cut(s) 371
PauI GCGCGC 1 cut(s) 199
PcsI WCGNNNNNNNCGW 1 cut(s) 890
PfeI GAWTC 1 cut(s) 823
PflFI GACNNNGTC 1 cut(s) 926
PkrI GCNGC 3 cut(s) 7, 617, 1025
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PspN4I GGNNCC 2 cut(s) 579, 942
PspPI GGNCC 2 cut(s) 779, 911
PstI CTGCAG 1 cut(s) 10
PsyI GACNNNGTC 1 cut(s) 926
PteI GCGCGC 1 cut(s) 199
RsaI GTAC 4 cut(s) 273, 420, 842, 980
RsaNI GTAC 4 cut(s) 272, 419, 841, 979
RseI CAYNNNNRTG 1 cut(s) 623
SaqAI TTAA 4 cut(s) 108, 261, 351, 801
SatI GCNGC 3 cut(s) 6, 616, 1024
Sau3AI GATC 6 cut(s) 127, 207, 217, 610, 694, 1081
Sau96I GGNCC 2 cut(s) 779, 911
ScaI AGTACT 1 cut(s) 420
SchI GAGTC 1 cut(s) 71
SduI GDGCHC 1 cut(s) 753
SfaNI GCATC 3 cut(s) 54, 843, 865
SfcI CTRYAG 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 371
SfuI TTCGAA 1 cut(s) 448
SinI GGWCC 1 cut(s) 911
SlaI CTCGAG 1 cut(s) 371
SmiMI CAYNNNNRTG 1 cut(s) 623
SmlI CTYRAG 2 cut(s) 15, 371
SmoI CTYRAG 2 cut(s) 15, 371
SphI GCATGC 2 cut(s) 309, 671
SsiI CCGC 3 cut(s) 214, 615, 1023
SspMI CTAG 7 cut(s) 56, 404, 486, 629, 704, 1091, 1096
StyI CCWWGG 2 cut(s) 21, 310
TaaI ACNGT 4 cut(s) 772, 836, 868, 928
TaqI TCGA 4 cut(s) 372, 448, 747, 893
TatI WGTACW 2 cut(s) 418, 840
TauI GCSGC 2 cut(s) 618, 1026
TfiI GAWTC 1 cut(s) 823
Tru1I TTAA 4 cut(s) 108, 261, 351, 801
Tru9I TTAA 4 cut(s) 108, 261, 351, 801
TscAI CASTG 3 cut(s) 499, 543, 775
TseFI GTSAC 3 cut(s) 37, 63, 706
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 3 cut(s) 37, 63, 706
TspDTI ATGAA 7 cut(s) 241, 302, 617, 741, 746, 1004, 1082
TspGWI ACGGA 2 cut(s) 282, 907
TspRI CASTG 3 cut(s) 499, 543, 775
Tth111I GACNNNGTC 1 cut(s) 926
VpaK11BI GGWCC 1 cut(s) 911
XagI CCTNNNNNAGG 1 cut(s) 287
XceI RCATGY 2 cut(s) 309, 671
XhoI CTCGAG 1 cut(s) 371
XmiI GTMKAC 2 cut(s) 679, 921
XspI CTAG 7 cut(s) 56, 404, 486, 629, 704, 1091, 1096
ZrmI AGTACT 1 cut(s) 420
Zsp2I ATGCAT 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.