FvH4_5g20430

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000481
Physical Location & Seq
Reverse (-)
12087531 .. 12089326
1796 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20430.t1

Sequence Viewer

Length: 1068 bp
ATGGCTGCAGTTGCTCAAGCGTTGGAGAACTCAAATGTCTCGAGCATCAAATCACTAGCTGACTCACAAGCTCTCAACTCTGTGCCTTCTGCATATGCCTTCAACATTAACCCCAATGATGAAGCTGATCCTAATGACCCTGAGTTTGCCATTCCCATTGTTGATATGTCTCTTCTCACTTCTGGTTCCCCTGCTCAGCGCGCCCAAATCGTCCACGATCTTGTCAAAATTTGTCAAGAATGGGGCTTCTTCATCGCAATTAACCACGGAGTACCAGAGAGTCTGATGAAGGCGATGATTGACGCATGCCATGGATTTTTCAGTCTTCCGGATGAGGAGAAGAAGGAGTTTAAATCAGGAAATGATGTTCTTGAGATGTTCAAGTATGGAACAAGCTATAATCTAGCATTGGACAAAGTACTTTTATGGAGGGACTTCTTCAAGGTCCGAACACATCCTGAGTTTTACTCTCTCTACAAGCCAGCTAGCTTCAGTGAGGTTTCGCTGGAGTTTAGCCAAAGAAGCAGAGAAGTAGCACTGGAAATAACAAGAGCAATATCAGAAAGCTTGGGGTTGGAGCCGGACTACATATATAACAAAATGAACATGAATCGCGGCCTACAAATGCTAGCAGGGAACTACTACCCACCTTGCCCTGAGCCTGAGCATGCCATCGGTATACCCCATCATACCGATCATGGTTTAGTCACACTCCTCATCCAGAATGAGATGAATGGTCTCCAAGTTGAGCACAATGGAAAATGGCTCACAGTCAATGGCCCTAAGAATGGTTTTTTTGTTAACCTTGCAGACCAAATGCAGATCCTTACAAACGGTAAGTACAAGAGTGTGATGCATCGCGCCACCGTGAACAACAAAGCTACGAGGATATCAATAGCCATACCACATGGACCATCCGTAGACACGATCATCGCACCAGCAGCAGAGTTGTGTGAAAGAGAAGGCCAAGCACCAAAGTACCTTGCAATGAACTACAAGGAATACATCCAACTTCAGCAAAGCGGCAAGAACTACATGAAGTCCACATTTGATCACATCCGAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

356

Amino Acids

39.78

Weight (kDa)

5.75

Isoelectric Point (pI)

35.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 51 - 155 1.6e-25 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 208 - 302 2.8e-29 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 679, 921
AccII CGCG 3 cut(s) 201, 615, 861
AccIII TCCGGA 1 cut(s) 328
AciI CCGC 2 cut(s) 615, 1023
AclWI GGATC 2 cut(s) 122, 817
AcsI RAATTY 1 cut(s) 228
AcuI CTGAAG 2 cut(s) 475, 998
AfaI GTAC 4 cut(s) 273, 420, 842, 980
AfiI CCNNNNNNNGG 1 cut(s) 788
AgsI TTSAA 3 cut(s) 103, 382, 442
AloI GAACNNNNNNTCC 2 cut(s) 351, 383
AluBI AGCT 8 cut(s) 59, 71, 125, 396, 485, 489, 567, 881
AluI AGCT 8 cut(s) 59, 71, 125, 396, 485, 489, 567, 881
Alw21I GWGCWC 1 cut(s) 753
Alw26I GTCTC 3 cut(s) 43, 174, 743
AlwI GGATC 2 cut(s) 122, 817
Ama87I CYCGRG 1 cut(s) 40
Aor13HI TCCGGA 1 cut(s) 328
AoxI GGCC 3 cut(s) 616, 778, 964
ApeKI GCWGC 2 cut(s) 5, 941
ApoI RAATTY 1 cut(s) 228
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AspLEI GCGC 3 cut(s) 201, 203, 863
AspS9I GGNCC 3 cut(s) 445, 779, 911
AsuNHI GCTAGC 2 cut(s) 485, 628
AvaI CYCGRG 1 cut(s) 40
AvaII GGWCC 2 cut(s) 445, 911
BaeI ACNNNNGTAYC 2 cut(s) 962, 995
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 1 cut(s) 753
BbvI GCAGC 1 cut(s) 953
BccI CCATC 3 cut(s) 680, 693, 922
BclI TGATCA 1 cut(s) 1051
BcoDI GTCTC 3 cut(s) 43, 174, 743
BfaI CTAG 4 cut(s) 56, 404, 486, 629
BfmI CTRYAG 1 cut(s) 6
BisI GCNGC 4 cut(s) 6, 616, 942, 1024
BlpI GCTNAGC 1 cut(s) 195
BlsI GCNGC 4 cut(s) 7, 617, 943, 1025
BmcAI AGTACT 1 cut(s) 420
Bme18I GGWCC 2 cut(s) 445, 911
BmeT110I CYCGRG 1 cut(s) 40
BmgT120I GGNCC 3 cut(s) 445, 779, 911
BmiI GGNNCC 2 cut(s) 187, 579
BmsI GCATC 3 cut(s) 54, 843, 865
BmtI GCTAGC 2 cut(s) 489, 632
BpiI GAAGAC 1 cut(s) 317
BplI GAGNNNNNCTC 2 cut(s) 452, 484
BpmI CTGGAG 1 cut(s) 527
Bpu10I CCTNAGC 2 cut(s) 657, 663
Bpu1102I GCTNAGC 1 cut(s) 195
BpuEI CTTGAG 1 cut(s) 392
BsaI GGTCTC 1 cut(s) 743
BsaJI CCNNGG 2 cut(s) 265, 310
BsaWI WCCGGW 1 cut(s) 328
Bsc4I CCNNNNNNNGG 1 cut(s) 788
Bse1I ACTGG 1 cut(s) 543
Bse3DI GCAATG 1 cut(s) 993
BseAI TCCGGA 1 cut(s) 328
BseDI CCNNGG 2 cut(s) 265, 310
BseGI GGATG 6 cut(s) 337, 454, 717, 914, 1005, 1056
BseLI CCNNNNNNNGG 1 cut(s) 788
BseMI GCAATG 1 cut(s) 993
BseMII CTCAG 5 cut(s) 132, 209, 450, 648, 654
BseNI ACTGG 1 cut(s) 543
BsePI GCGCGC 1 cut(s) 199
BseRI GAGGAG 2 cut(s) 350, 704
BseXI GCAGC 1 cut(s) 953
Bsh1236I CGCG 3 cut(s) 201, 615, 861
BshFI GGCC 3 cut(s) 618, 780, 966
BsiHKAI GWGCWC 1 cut(s) 753
BsiHKCI CYCGRG 1 cut(s) 40
BsiSI CCGG 2 cut(s) 329, 581
BslFI GGGAC 1 cut(s) 446
BslI CCNNNNNNNGG 1 cut(s) 788
BsmAI GTCTC 3 cut(s) 43, 174, 743
BsmFI GGGAC 1 cut(s) 446
BsnI GGCC 3 cut(s) 618, 780, 966
Bso31I GGTCTC 1 cut(s) 743
BsoBI CYCGRG 1 cut(s) 40
Bsp1286I GDGCHC 1 cut(s) 753
Bsp13I TCCGGA 1 cut(s) 328
Bsp143I GATC 6 cut(s) 127, 217, 694, 822, 927, 1051
Bsp1720I GCTNAGC 1 cut(s) 195
Bsp19I CCATGG 1 cut(s) 310
BspACI CCGC 2 cut(s) 615, 1023
BspANI GGCC 3 cut(s) 618, 780, 966
BspCNI CTCAG 5 cut(s) 133, 208, 451, 649, 655
BspEI TCCGGA 1 cut(s) 328
BspFNI CGCG 3 cut(s) 201, 615, 861
BspLI GGNNCC 2 cut(s) 187, 579
BspMAI CTGCAG 1 cut(s) 10
BspOI GCTAGC 2 cut(s) 489, 632
BspPI GGATC 2 cut(s) 122, 817
BspTNI GGTCTC 1 cut(s) 743
BsrDI GCAATG 1 cut(s) 993
BsrI ACTGG 1 cut(s) 543
BssECI CCNNGG 2 cut(s) 265, 310
BssHII GCGCGC 1 cut(s) 199
BssMI GATC 6 cut(s) 127, 217, 694, 822, 927, 1051
BssNAI GTATAC 1 cut(s) 680
BssT1I CCWWGG 1 cut(s) 310
Bst1107I GTATAC 1 cut(s) 680
Bst4CI ACNGT 3 cut(s) 772, 836, 868
Bst6I CTCTTC 1 cut(s) 177
BstC8I GCNNGC 6 cut(s) 201, 307, 483, 487, 630, 669
BstDEI CTNAG 7 cut(s) 141, 195, 459, 657, 663, 783, 1065
BstDSI CCRYGG 2 cut(s) 265, 310
BstF5I GGATG 6 cut(s) 337, 454, 717, 914, 1005, 1056
BstFNI CGCG 3 cut(s) 201, 615, 861
BstHHI GCGC 3 cut(s) 201, 203, 863
BstKTI GATC 6 cut(s) 130, 220, 697, 825, 930, 1054
BstMAI GTCTC 3 cut(s) 43, 174, 743
BstMBI GATC 6 cut(s) 127, 217, 694, 822, 927, 1051
BstMWI GCNNNNNNNGC 4 cut(s) 11, 200, 522, 941
BstNSI RCATGY 2 cut(s) 309, 671
BstSFI CTRYAG 1 cut(s) 6
BstUI CGCG 3 cut(s) 201, 615, 861
BstV1I GCAGC 1 cut(s) 953
BstV2I GAAGAC 1 cut(s) 317
BstX2I RGATCY 1 cut(s) 822
BstYI RGATCY 1 cut(s) 822
BstZ17I GTATAC 1 cut(s) 680
BsuRI GGCC 3 cut(s) 618, 780, 966
BtgI CCRYGG 2 cut(s) 265, 310
BtgZI GCGATG 4 cut(s) 238, 308, 842, 916
BtsCI GGATG 6 cut(s) 337, 454, 717, 914, 1005, 1056
BtsIMutI CAGTG 2 cut(s) 499, 536
Cac8I GCNNGC 6 cut(s) 201, 307, 483, 487, 630, 669
CfoI GCGC 3 cut(s) 201, 203, 863
Cfr13I GGNCC 3 cut(s) 445, 779, 911
CseI GACGC 1 cut(s) 311
Csp6I GTAC 4 cut(s) 272, 419, 841, 979
CviAII CATG 7 cut(s) 306, 311, 607, 668, 698, 908, 1036
CviQI GTAC 4 cut(s) 272, 419, 841, 979
DdeI CTNAG 7 cut(s) 141, 195, 459, 657, 663, 783, 1065
DpnI GATC 6 cut(s) 129, 219, 696, 824, 929, 1053
DpnII GATC 6 cut(s) 127, 217, 694, 822, 927, 1051
DraI TTTAAA 1 cut(s) 352
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Eco130I CCWWGG 1 cut(s) 310
Eco31I GGTCTC 1 cut(s) 743
Eco32I GATATC 1 cut(s) 891
Eco47I GGWCC 2 cut(s) 445, 911
Eco57I CTGAAG 2 cut(s) 475, 998
Eco88I CYCGRG 1 cut(s) 40
EcoRV GATATC 1 cut(s) 891
EcoT14I CCWWGG 1 cut(s) 310
EcoT22I ATGCAT 1 cut(s) 858
ErhI CCWWGG 1 cut(s) 310
FaeI CATG 7 cut(s) 309, 314, 610, 671, 701, 911, 1039
FaqI GGGAC 1 cut(s) 446
FatI CATG 7 cut(s) 305, 310, 606, 667, 697, 907, 1035
FauNDI CATATG 1 cut(s) 94
FbaI TGATCA 1 cut(s) 1051
FblI GTMKAC 2 cut(s) 679, 921
Fnu4HI GCNGC 4 cut(s) 6, 616, 942, 1024
FokI GGATG 6 cut(s) 344, 441, 704, 901, 992, 1043
Fsp4HI GCNGC 4 cut(s) 6, 616, 942, 1024
FspBI CTAG 4 cut(s) 56, 404, 486, 629
GlaI GCGC 3 cut(s) 200, 202, 862
GluI GCNGC 4 cut(s) 6, 616, 942, 1024
GsuI CTGGAG 1 cut(s) 527
HaeIII GGCC 3 cut(s) 618, 780, 966
HapII CCGG 2 cut(s) 329, 581
HgaI GACGC 1 cut(s) 311
HhaI GCGC 3 cut(s) 201, 203, 863
Hin1II CATG 7 cut(s) 309, 314, 610, 671, 701, 911, 1039
Hin6I GCGC 3 cut(s) 199, 201, 861
HinP1I GCGC 3 cut(s) 199, 201, 861
HincII GTYRAC 1 cut(s) 802
HindII GTYRAC 1 cut(s) 802
HindIII AAGCTT 1 cut(s) 565
HinfI GANTC 3 cut(s) 62, 280, 610
HpaI GTTAAC 1 cut(s) 802
HpaII CCGG 2 cut(s) 329, 581
Hpy166II GTNNAC 6 cut(s) 214, 680, 802, 871, 922, 1044
Hpy188I TCNGA 4 cut(s) 285, 449, 562, 1061
Hpy188III TCNNGA 7 cut(s) 40, 236, 329, 357, 371, 458, 721
Hpy8I GTNNAC 6 cut(s) 214, 680, 802, 871, 922, 1044
HpyAV CCTTC 5 cut(s) 96, 109, 283, 337, 956
HpyCH4III ACNGT 3 cut(s) 772, 836, 868
HpyCH4V TGCA 6 cut(s) 8, 92, 809, 820, 856, 986
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 200, 522, 941
HpyF3I CTNAG 7 cut(s) 141, 195, 459, 657, 663, 783, 1065
Hsp92II CATG 7 cut(s) 309, 314, 610, 671, 701, 911, 1039
HspAI GCGC 3 cut(s) 199, 201, 861
Kpn2I TCCGGA 1 cut(s) 328
Ksp22I TGATCA 1 cut(s) 1051
KspAI GTTAAC 1 cut(s) 802
Kzo9I GATC 6 cut(s) 127, 217, 694, 822, 927, 1051
LmnI GCTCC 1 cut(s) 577
Lsp1109I GCAGC 1 cut(s) 953
LweI GCATC 3 cut(s) 54, 843, 865
MaeI CTAG 4 cut(s) 56, 404, 486, 629
MaeIII GTNAC 1 cut(s) 706
MalI GATC 6 cut(s) 129, 219, 696, 824, 929, 1053
MboI GATC 6 cut(s) 127, 217, 694, 822, 927, 1051
MboII GAAGA 5 cut(s) 164, 241, 317, 352, 430
MflI RGATCY 1 cut(s) 822
MhlI GDGCHC 1 cut(s) 753
MluCI AATT 2 cut(s) 228, 258
MlyI GAGTC 2 cut(s) 56, 289
MmeI TCCRAC 2 cut(s) 555, 1033
MnlI CCTC 5 cut(s) 328, 423, 490, 725, 879
Mph1103I ATGCAT 1 cut(s) 858
MroI TCCGGA 1 cut(s) 328
MseI TTAA 4 cut(s) 108, 261, 351, 801
MspI CCGG 2 cut(s) 329, 581
MvnI CGCG 3 cut(s) 201, 615, 861
MwoI GCNNNNNNNGC 4 cut(s) 11, 200, 522, 941
NcoI CCATGG 1 cut(s) 310
NdeI CATATG 1 cut(s) 94
NdeII GATC 6 cut(s) 127, 217, 694, 822, 927, 1051
NheI GCTAGC 2 cut(s) 485, 628
NlaIII CATG 7 cut(s) 309, 314, 610, 671, 701, 911, 1039
NlaIV GGNNCC 2 cut(s) 187, 579
NmuCI GTSAC 1 cut(s) 706
NsiI ATGCAT 1 cut(s) 858
NspI RCATGY 2 cut(s) 309, 671
PaeI GCATGC 2 cut(s) 309, 671
PaeR7I CTCGAG 1 cut(s) 40
PauI GCGCGC 1 cut(s) 199
PfeI GAWTC 1 cut(s) 610
PkrI GCNGC 4 cut(s) 7, 617, 943, 1025
PleI GAGTC 2 cut(s) 56, 288
PpsI GAGTC 2 cut(s) 56, 288
PspN4I GGNNCC 2 cut(s) 187, 579
PspPI GGNCC 3 cut(s) 445, 779, 911
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 1 cut(s) 822
PteI GCGCGC 1 cut(s) 199
RsaI GTAC 4 cut(s) 273, 420, 842, 980
RsaNI GTAC 4 cut(s) 272, 419, 841, 979
SaqAI TTAA 4 cut(s) 108, 261, 351, 801
SatI GCNGC 4 cut(s) 6, 616, 942, 1024
Sau3AI GATC 6 cut(s) 127, 217, 694, 822, 927, 1051
Sau96I GGNCC 3 cut(s) 445, 779, 911
ScaI AGTACT 1 cut(s) 420
SchI GAGTC 2 cut(s) 56, 289
SduI GDGCHC 1 cut(s) 753
SfaNI GCATC 3 cut(s) 54, 843, 865
SfcI CTRYAG 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 40
SinI GGWCC 2 cut(s) 445, 911
SlaI CTCGAG 1 cut(s) 40
SmlI CTYRAG 3 cut(s) 15, 40, 371
SmoI CTYRAG 3 cut(s) 15, 40, 371
SphI GCATGC 2 cut(s) 309, 671
Sse9I AATT 2 cut(s) 228, 258
SsiI CCGC 2 cut(s) 615, 1023
SspMI CTAG 4 cut(s) 56, 404, 486, 629
StyI CCWWGG 1 cut(s) 310
TaaI ACNGT 3 cut(s) 772, 836, 868
TaqI TCGA 1 cut(s) 41
TasI AATT 2 cut(s) 228, 258
TatI WGTACW 2 cut(s) 418, 840
TauI GCSGC 2 cut(s) 618, 1026
TfiI GAWTC 1 cut(s) 610
Tru1I TTAA 4 cut(s) 108, 261, 351, 801
Tru9I TTAA 4 cut(s) 108, 261, 351, 801
TscAI CASTG 2 cut(s) 499, 543
TseFI GTSAC 1 cut(s) 706
TseI GCWGC 2 cut(s) 5, 941
Tsp45I GTSAC 1 cut(s) 706
TspDTI ATGAA 8 cut(s) 135, 241, 302, 617, 623, 746, 1004, 1052
TspGWI ACGGA 2 cut(s) 282, 907
TspRI CASTG 2 cut(s) 499, 543
VpaK11BI GGWCC 2 cut(s) 445, 911
XapI RAATTY 1 cut(s) 228
XceI RCATGY 2 cut(s) 309, 671
XhoI CTCGAG 1 cut(s) 40
XmiI GTMKAC 2 cut(s) 679, 921
XspI CTAG 4 cut(s) 56, 404, 486, 629
ZrmI AGTACT 1 cut(s) 420
Zsp2I ATGCAT 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.