Rw5G042000

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000481
Physical Location & Seq
Reverse (-)
73649179 .. 73650682
1504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G042000.1

Sequence Viewer

Length: 1023 bp
ATGGCGCCAGCAATAGCTCAAAATGTGGAGACATCGACTGTCACTCCAACCAAAGTGACTAGCATTAAAACACTTGCTGAGAGTTCAGCTGCTCTCAGCTCTGTACCTTCTGCATACGCCTTTACCATAAATCCCAACGATGAAGCTGATCCTAATGACCCCGAATTCGCAATTCCAATTATCGATATGTCTCTTCTAACCTGCGGTTCACCTGATCAACGCTCCAAGATCATCCAAGACCTTGTCAAAATTTGTCAAGAATGGGGATTCTTCATAGTACTGCCAGAGAGCCTAATGAAGGCGATGATCGATTCATGTCATGGATTTTTCAGTCTTCCGGAGAGTGAGAAGCAGGAGTTTAAATCAGGAAACGATGTGCTGGAGATGTTCAAGTATGGGACTAGCTATAATCTTGCATTGGACAAAGTCCTTCTCTGGAGGGACTTCTTCAAGATATCATTGGAGTTCAGAAAAAGAACAAGAGAAGTAGCACTGGAAATCATCAAAGCTATATCGGAAAGCCTGGGATTGGAGCCCAACTACATATATGATGCCATGAATATGGATCGGGGCTTACAAATGCTAGCCGGGAACTACTACCCTCTTTGCCCTCAGCCAGAACATGCAATTGGTATACCACATCATACAGATCATGGTCTAGTGACACTTCTCATTCAGAATGAGATGAAGGGTCTCCAAGTCAAACACAATGGGAAATGGCTCACTGTCGATGGCCCTGCAAACGGTTTTTTTGTTAATCTTGCTGACCAAATGCAGATTCTTACAAACGGAAAGTACAAGAGTGTGATGCACCGGGCAATTGTGAATAACAAAGCTGCAAGGATATCTATAGCCATACCACATGGACCATCTGTAGATACAACTATTGCACCTTCACCAGAATTGTGTGACAGAGAAGGTCAGGCTCCAAAATACCTTGGAATGAACTACAAGGAATACATACAACTTCAACAAAGTGGCAAGAATTACATGAAGTCAACCTTCGATCACATACGAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

340

Amino Acids

37.99

Weight (kDa)

5.87

Isoelectric Point (pI)

38.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 58 - 158 4.8e-18 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 193 - 287 2.6e-27 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 209
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 634
AccIII TCCGGA 1 cut(s) 337
AciI CCGC 1 cut(s) 204
AclWI GGATC 2 cut(s) 143, 573
AcsI RAATTY 2 cut(s) 164, 249
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 105, 279, 797
AfiI CCNNNNNNNGG 3 cut(s) 298, 529, 743
AgsI TTSAA 3 cut(s) 391, 451, 971
AjnI CCWGG 1 cut(s) 522
AjuI GAANNNNNNNTTGG 4 cut(s) 612, 644, 762, 794
AluBI AGCT 7 cut(s) 17, 89, 99, 146, 405, 509, 836
AluI AGCT 7 cut(s) 17, 89, 99, 146, 405, 509, 836
Alw26I GTCTC 3 cut(s) 23, 195, 698
AlwI GGATC 2 cut(s) 143, 573
Aor13HI TCCGGA 1 cut(s) 337
AoxI GGCC 1 cut(s) 733
ApeKI GCWGC 2 cut(s) 89, 836
ApoI RAATTY 2 cut(s) 164, 249
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 734, 866
AsuC2I CCSGG 2 cut(s) 589, 815
AsuHPI GGTGA 2 cut(s) 201, 888
AsuNHI GCTAGC 1 cut(s) 583
AvaII GGWCC 1 cut(s) 866
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 537
BbsI GAAGAC 1 cut(s) 326
BbvCI CCTCAGC 1 cut(s) 612
BbvI GCAGC 2 cut(s) 76, 823
BccI CCATC 2 cut(s) 725, 877
BcgI CGANNNNNNTGC 2 cut(s) 719, 753
BciT130I CCWGG 1 cut(s) 524
BclI TGATCA 1 cut(s) 214
BcnI CCSGG 2 cut(s) 589, 815
BcoDI GTCTC 3 cut(s) 23, 195, 698
BfaI CTAG 5 cut(s) 60, 402, 584, 659, 1021
BfmI CTRYAG 2 cut(s) 849, 873
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 209
BisI GCNGC 2 cut(s) 90, 837
BlsI GCNGC 2 cut(s) 91, 838
BmcAI AGTACT 1 cut(s) 279
Bme1390I CCNGG 3 cut(s) 524, 589, 815
Bme18I GGWCC 1 cut(s) 866
BmgT120I GGNCC 2 cut(s) 734, 866
BmiI GGNNCC 3 cut(s) 6, 534, 927
BmrFI CCNGG 3 cut(s) 524, 589, 815
BmsI GCATC 2 cut(s) 541, 798
BmtI GCTAGC 1 cut(s) 587
BpiI GAAGAC 1 cut(s) 326
BpmI CTGGAG 2 cut(s) 401, 457
Bpu10I CCTNAGC 1 cut(s) 612
BpuMI CCSGG 2 cut(s) 589, 815
Bsa29I ATCGAT 2 cut(s) 183, 309
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 698
BsaJI CCNNGG 2 cut(s) 523, 937
BsaWI WCCGGW 1 cut(s) 337
BsaXI ACNNNNNCTCC 2 cut(s) 28, 58
Bsc4I CCNNNNNNNGG 3 cut(s) 298, 529, 743
Bse1I ACTGG 1 cut(s) 498
BseAI TCCGGA 1 cut(s) 337
BseBI CCWGG 1 cut(s) 524
BseCI ATCGAT 2 cut(s) 183, 309
BseDI CCNNGG 2 cut(s) 523, 937
BseGI GGATG 1 cut(s) 231
BseLI CCNNNNNNNGG 3 cut(s) 298, 529, 743
BseMII CTCAG 3 cut(s) 69, 109, 626
BseNI ACTGG 1 cut(s) 498
BseXI GCAGC 2 cut(s) 76, 823
BshFI GGCC 1 cut(s) 735
BshNI GGYRCC 1 cut(s) 4
BshVI ATCGAT 2 cut(s) 183, 309
BsiSI CCGG 3 cut(s) 338, 588, 814
BslFI GGGAC 2 cut(s) 412, 455
BslI CCNNNNNNNGG 3 cut(s) 298, 529, 743
BsmAI GTCTC 3 cut(s) 23, 195, 698
BsmFI GGGAC 2 cut(s) 412, 455
BsnI GGCC 1 cut(s) 735
Bso31I GGTCTC 1 cut(s) 698
Bsp1286I GDGCHC 1 cut(s) 537
Bsp13I TCCGGA 1 cut(s) 337
Bsp143I GATC 7 cut(s) 148, 214, 228, 306, 565, 649, 1006
BspACI CCGC 1 cut(s) 204
BspANI GGCC 1 cut(s) 735
BspCNI CTCAG 3 cut(s) 70, 108, 625
BspDI ATCGAT 2 cut(s) 183, 309
BspEI TCCGGA 1 cut(s) 337
BspLI GGNNCC 3 cut(s) 6, 534, 927
BspMI ACCTGC 1 cut(s) 209
BspOI GCTAGC 1 cut(s) 587
BspPI GGATC 2 cut(s) 143, 573
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 698
BsrI ACTGG 1 cut(s) 498
BssECI CCNNGG 2 cut(s) 523, 937
BssMI GATC 7 cut(s) 148, 214, 228, 306, 565, 649, 1006
BssNAI GTATAC 1 cut(s) 635
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 937
Bst1107I GTATAC 1 cut(s) 635
Bst2UI CCWGG 1 cut(s) 524
Bst4CI ACNGT 3 cut(s) 40, 727, 746
Bst6I CTCTTC 1 cut(s) 198
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 2 cut(s) 9, 585
BstDEI CTNAG 3 cut(s) 78, 95, 612
BstENI CCTNNNNNAGG 1 cut(s) 296
BstF5I GGATG 1 cut(s) 231
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 7 cut(s) 151, 217, 231, 309, 568, 652, 1009
BstMAI GTCTC 3 cut(s) 23, 195, 698
BstMBI GATC 7 cut(s) 148, 214, 228, 306, 565, 649, 1006
BstNI CCWGG 1 cut(s) 524
BstNSI RCATGY 1 cut(s) 626
BstSCI CCNGG 3 cut(s) 522, 587, 813
BstSFI CTRYAG 2 cut(s) 849, 873
BstV1I GCAGC 2 cut(s) 76, 823
BstV2I GAAGAC 1 cut(s) 326
BstXI CCANNNNNNTGG 1 cut(s) 562
BstZ17I GTATAC 1 cut(s) 635
Bsu15I ATCGAT 2 cut(s) 183, 309
BsuRI GGCC 1 cut(s) 735
BsuTUI ATCGAT 2 cut(s) 183, 309
BtgZI GCGATG 1 cut(s) 317
BtsCI GGATG 1 cut(s) 231
BtsIMutI CAGTG 2 cut(s) 491, 723
BveI ACCTGC 1 cut(s) 209
Cac8I GCNNGC 2 cut(s) 9, 585
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 734, 866
ClaI ATCGAT 2 cut(s) 183, 309
Csp6I GTAC 3 cut(s) 104, 278, 796
CviAII CATG 7 cut(s) 315, 320, 556, 623, 653, 863, 991
CviQI GTAC 3 cut(s) 104, 278, 796
DdeI CTNAG 3 cut(s) 78, 95, 612
DinI GGCGCC 1 cut(s) 6
DpnI GATC 7 cut(s) 150, 216, 230, 308, 567, 651, 1008
DpnII GATC 7 cut(s) 148, 214, 228, 306, 565, 649, 1006
DraI TTTAAA 1 cut(s) 361
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco130I CCWWGG 1 cut(s) 937
Eco24I GRGCYC 1 cut(s) 537
Eco31I GGTCTC 1 cut(s) 698
Eco32I GATATC 2 cut(s) 456, 846
Eco47I GGWCC 1 cut(s) 866
EcoNI CCTNNNNNAGG 1 cut(s) 296
EcoRI GAATTC 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 522
EcoRV GATATC 2 cut(s) 456, 846
EcoT14I CCWWGG 1 cut(s) 937
EcoT38I GRGCYC 1 cut(s) 537
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 937
FaeI CATG 7 cut(s) 318, 323, 559, 626, 656, 866, 994
FalI AAGNNNNNCTT 2 cut(s) 986, 1018
FaqI GGGAC 2 cut(s) 412, 455
FatI CATG 7 cut(s) 314, 319, 555, 622, 652, 862, 990
FbaI TGATCA 1 cut(s) 214
FblI GTMKAC 1 cut(s) 634
Fnu4HI GCNGC 2 cut(s) 90, 837
FokI GGATG 1 cut(s) 218
FriOI GRGCYC 1 cut(s) 537
Fsp4HI GCNGC 2 cut(s) 90, 837
FspBI CTAG 5 cut(s) 60, 402, 584, 659, 1021
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 90, 837
GsuI CTGGAG 2 cut(s) 401, 457
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 735
HapII CCGG 3 cut(s) 338, 588, 814
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 7 cut(s) 318, 323, 559, 626, 656, 866, 994
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 999
HindII GTYRAC 1 cut(s) 999
HinfI GANTC 4 cut(s) 267, 311, 778, 1017
HpaII CCGG 3 cut(s) 338, 588, 814
HphI GGTGA 2 cut(s) 201, 888
Hpy166II GTNNAC 3 cut(s) 209, 635, 999
Hpy188I TCNGA 3 cut(s) 470, 517, 678
Hpy188III TCNNGA 5 cut(s) 257, 338, 366, 436, 451
Hpy8I GTNNAC 3 cut(s) 209, 635, 999
HpyAV CCTTC 7 cut(s) 117, 292, 440, 682, 903, 911, 1012
HpyCH4III ACNGT 3 cut(s) 40, 727, 746
HpyCH4V TGCA 8 cut(s) 113, 416, 626, 740, 775, 811, 839, 890
HpyF3I CTNAG 3 cut(s) 78, 95, 612
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 7 cut(s) 318, 323, 559, 626, 656, 866, 994
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Kpn2I TCCGGA 1 cut(s) 337
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 7 cut(s) 148, 214, 228, 306, 565, 649, 1006
LmnI GCTCC 3 cut(s) 227, 532, 931
Lsp1109I GCAGC 2 cut(s) 76, 823
LweI GCATC 2 cut(s) 541, 798
MaeI CTAG 5 cut(s) 60, 402, 584, 659, 1021
MaeIII GTNAC 4 cut(s) 40, 55, 661, 908
MalI GATC 7 cut(s) 150, 216, 230, 308, 567, 651, 1008
MboI GATC 7 cut(s) 148, 214, 228, 306, 565, 649, 1006
MboII GAAGA 4 cut(s) 185, 262, 326, 439
MfeI CAATTG 2 cut(s) 627, 819
MhlI GDGCHC 1 cut(s) 537
MluCI AATT 8 cut(s) 164, 171, 177, 249, 627, 819, 902, 985
Mly113I GGCGCC 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 71
MnlI CCTC 3 cut(s) 432, 612, 621
MroI TCCGGA 1 cut(s) 337
MseI TTAA 3 cut(s) 66, 360, 756
MslI CAYNNNNRTG 1 cut(s) 560
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 3 cut(s) 338, 588, 814
MspR9I CCNGG 3 cut(s) 524, 589, 815
MunI CAATTG 2 cut(s) 627, 819
MvaI CCWGG 1 cut(s) 524
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 2 cut(s) 589, 815
NdeII GATC 7 cut(s) 148, 214, 228, 306, 565, 649, 1006
NheI GCTAGC 1 cut(s) 583
NlaIII CATG 7 cut(s) 318, 323, 559, 626, 656, 866, 994
NlaIV GGNNCC 3 cut(s) 6, 534, 927
NmuCI GTSAC 4 cut(s) 40, 55, 661, 908
NspI RCATGY 1 cut(s) 626
PfeI GAWTC 3 cut(s) 267, 311, 778
PflFI GACNNNGTC 2 cut(s) 242, 425
PkrI GCNGC 2 cut(s) 91, 838
PluTI GGCGCC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 522
PspGI CCWGG 1 cut(s) 522
PspN4I GGNNCC 3 cut(s) 6, 534, 927
PspPI GGNCC 2 cut(s) 734, 866
PsyI GACNNNGTC 2 cut(s) 242, 425
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 3 cut(s) 105, 279, 797
RsaNI GTAC 3 cut(s) 104, 278, 796
RseI CAYNNNNRTG 1 cut(s) 560
SaqAI TTAA 3 cut(s) 66, 360, 756
SatI GCNGC 2 cut(s) 90, 837
Sau3AI GATC 7 cut(s) 148, 214, 228, 306, 565, 649, 1006
Sau96I GGNCC 2 cut(s) 734, 866
ScaI AGTACT 1 cut(s) 279
ScrFI CCNGG 3 cut(s) 524, 589, 815
SduI GDGCHC 1 cut(s) 537
SfaNI GCATC 2 cut(s) 541, 798
SfcI CTRYAG 2 cut(s) 849, 873
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 866
SmiMI CAYNNNNRTG 1 cut(s) 560
Sse9I AATT 8 cut(s) 164, 171, 177, 249, 627, 819, 902, 985
SsiI CCGC 1 cut(s) 204
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 5 cut(s) 60, 402, 584, 659, 1021
StyD4I CCNGG 3 cut(s) 522, 587, 813
StyI CCWWGG 1 cut(s) 937
TaaI ACNGT 3 cut(s) 40, 727, 746
TaqI TCGA 5 cut(s) 35, 183, 309, 729, 1005
TasI AATT 8 cut(s) 164, 171, 177, 249, 627, 819, 902, 985
TatI WGTACW 2 cut(s) 277, 795
TfiI GAWTC 3 cut(s) 267, 311, 778
Tru1I TTAA 3 cut(s) 66, 360, 756
Tru9I TTAA 3 cut(s) 66, 360, 756
TscAI CASTG 2 cut(s) 498, 730
TseFI GTSAC 4 cut(s) 40, 55, 661, 908
TseI GCWGC 2 cut(s) 89, 836
Tsp45I GTSAC 4 cut(s) 40, 55, 661, 908
TspDTI ATGAA 8 cut(s) 156, 262, 303, 311, 572, 701, 959, 1007
TspGWI ACGGA 1 cut(s) 804
TspRI CASTG 2 cut(s) 498, 730
Tth111I GACNNNGTC 2 cut(s) 242, 425
VpaK11BI GGWCC 1 cut(s) 866
XagI CCTNNNNNAGG 1 cut(s) 296
XapI RAATTY 2 cut(s) 164, 249
XceI RCATGY 1 cut(s) 626
XmiI GTMKAC 1 cut(s) 634
XspI CTAG 5 cut(s) 60, 402, 584, 659, 1021
ZrmI AGTACT 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.