MD03G1140400.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000481
Physical Location & Seq
Forward (+)
14663051 .. 14664555
1505 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1140400.v1.1.491

Sequence Viewer

Length: 1062 bp
ATGGCTCCAGTTGCTACTCAAACCATGGATGTAAAGAGCATCAAATCGCTTGCGGAATCACCCGCTCTCTGCTCTGTTCCTTCTGCTTATGCCTTCAACATAAACCCCAATGATGAAGCAGATCCAAACGATCCCGAATTTGCAATCCCTATAGTTGATATGTCTCTTCTCACCTCGGGATCTCCTGATCAACGCTCCAAAATAATCCACGACCTGGTCAAAATTTGCAAAGAATGGGGCTTCTTCATTGCAATTAACCATGGAGTCCCAGAGAGTCTAATGAAGGGGATGATTGACGCGTGCCATGGATTTTTCAGCCTCCCAGACGAAGAGAAGGAGGAGTTCAAATCCGGAAATGATGTGCTGGACATGTTCAAGTACGGAACCAGCTACAATCTTGCATTAGACAAAGTCCTTCTCTGGAGGGACTTCTTCAAGGTGAGAGTACATCCCGAGTTCTACTCCCTCTACAAACCGGCTTGCTTCAGTGAGGTTTCAATGGAGTTCAGCAAGAGAACAAGAGAAGTAGCATTGGAGATAACACGCGCAATCTCCGAAAGTTTGGGACTGGAGCCCAACTACATACATGATGCGATGAACATGGATCGCGGCCTACAAATGCTCGCCGCCAACTACTATCCGCCTTGCCCTCAGCCAGAACATGCAATTGGTATACCACATCACACTGATCATGGCCTTGTCACCCTCCTCATCCAGAATGAGATGAATGGCCTCCAAGTTGAACATAACGGCAAGTGGCTCACCGTCAGTGGCGCTGCCAACGGTTTCTTTGTCAACCTCGCTGACCAAATGCAAATTCTTACTAATGGCAAATACAAGAGTGTGATGCATCGGGCAACTGTGAACAACAAAGCTACGAGGATATCTATTGCCATACCACATGGACCATCAATTGATACACTTATCGCACCGGCACCGGAGTTACTGGAAAAAGAAGGCCAAGCTCCGAAATATGTTGGAATGAATTATAAGGAATATATCCAGCTTCAACAGAGCGGCAAGAACTACATGAAATCCACCTTTGATCACATACTAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

354

Amino Acids

39.47

Weight (kDa)

5.62

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 49 - 152 7.6e-25 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 206 - 300 9.7e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 990
AccB1I GGYRCC 1 cut(s) 934
AccB7I CCANNNNNTGG 1 cut(s) 214
AccBSI CCGCTC 2 cut(s) 65, 1017
AccI GTMKAC 1 cut(s) 673
AccII CGCG 3 cut(s) 299, 546, 609
AccIII TCCGGA 1 cut(s) 350
AciI CCGC 6 cut(s) 53, 63, 609, 627, 641, 1017
AclWI GGATC 4 cut(s) 116, 125, 187, 612
AcsI RAATTY 3 cut(s) 137, 222, 816
AcuI CTGAAG 1 cut(s) 469
AfaI GTAC 2 cut(s) 380, 447
AfiI CCNNNNNNNGG 1 cut(s) 214
AflIII ACRYGT 2 cut(s) 297, 369
AgsI TTSAA 7 cut(s) 97, 346, 376, 436, 498, 743, 1010
AjnI CCWGG 1 cut(s) 213
AjuI GAANNNNNNNTTGG 4 cut(s) 651, 683, 801, 833
AluBI AGCT 4 cut(s) 390, 875, 965, 1006
AluI AGCT 4 cut(s) 390, 875, 965, 1006
Alw26I GTCTC 1 cut(s) 168
AlwI GGATC 4 cut(s) 116, 125, 187, 612
Ama87I CYCGRG 2 cut(s) 175, 452
Aor13HI TCCGGA 1 cut(s) 350
AoxI GGCC 4 cut(s) 610, 694, 730, 958
ApeKI GCWGC 1 cut(s) 776
ApoI RAATTY 3 cut(s) 137, 222, 816
AspLEI GCGC 2 cut(s) 548, 776
AspS9I GGNCC 1 cut(s) 905
AsuHPI GGTGA 5 cut(s) 51, 163, 451, 694, 754
AvaI CYCGRG 2 cut(s) 175, 452
AvaII GGWCC 1 cut(s) 905
BanI GGYRCC 1 cut(s) 934
BanII GRGCYC 1 cut(s) 576
BbvCI CCTCAGC 1 cut(s) 651
BbvI GCAGC 1 cut(s) 763
BccI CCATC 1 cut(s) 916
BceAI ACGGC 1 cut(s) 766
BciT130I CCWGG 1 cut(s) 215
BclI TGATCA 3 cut(s) 187, 688, 1045
BcoDI GTCTC 1 cut(s) 168
BfaI CTAG 1 cut(s) 1055
BfmI CTRYAG 1 cut(s) 150
BfoI RGCGCY 1 cut(s) 777
BisI GCNGC 4 cut(s) 610, 627, 777, 1018
BlsI GCNGC 4 cut(s) 611, 628, 778, 1019
Bme1390I CCNGG 1 cut(s) 215
Bme18I GGWCC 1 cut(s) 905
BmeT110I CYCGRG 2 cut(s) 175, 452
BmgT120I GGNCC 1 cut(s) 905
BmiI GGNNCC 4 cut(s) 6, 385, 573, 936
BmrFI CCNGG 1 cut(s) 215
BmsI GCATC 4 cut(s) 48, 580, 837, 859
BplI GAGNNNNNCTC 2 cut(s) 446, 478
BpmI CTGGAG 2 cut(s) 442, 590
Bpu10I CCTNAGC 1 cut(s) 651
BsaJI CCNNGG 4 cut(s) 24, 174, 259, 304
BsaWI WCCGGW 2 cut(s) 350, 937
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse118I RCCGGY 2 cut(s) 475, 931
Bse1I ACTGG 3 cut(s) 8, 573, 951
Bse3DI GCAATG 1 cut(s) 246
BseAI TCCGGA 1 cut(s) 350
BseBI CCWGG 1 cut(s) 215
BseDI CCNNGG 4 cut(s) 24, 174, 259, 304
BseGI GGATG 4 cut(s) 34, 294, 448, 711
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMI GCAATG 1 cut(s) 246
BseMII CTCAG 1 cut(s) 665
BseNI ACTGG 3 cut(s) 8, 573, 951
BseRI GAGGAG 2 cut(s) 353, 698
BseXI GCAGC 1 cut(s) 763
Bsh1236I CGCG 3 cut(s) 299, 546, 609
BshFI GGCC 4 cut(s) 612, 696, 732, 960
BshNI GGYRCC 1 cut(s) 934
BsiHKCI CYCGRG 2 cut(s) 175, 452
BsiSI CCGG 4 cut(s) 351, 476, 932, 938
BslFI GGGAC 3 cut(s) 251, 440, 579
BslI CCNNNNNNNGG 1 cut(s) 214
BsmAI GTCTC 1 cut(s) 168
BsmFI GGGAC 3 cut(s) 251, 440, 579
BsnI GGCC 4 cut(s) 612, 696, 732, 960
BsoBI CYCGRG 2 cut(s) 175, 452
Bsp1286I GDGCHC 1 cut(s) 576
Bsp13I TCCGGA 1 cut(s) 350
Bsp143I GATC 7 cut(s) 121, 130, 179, 187, 604, 688, 1045
Bsp19I CCATGG 3 cut(s) 24, 259, 304
BspACI CCGC 6 cut(s) 53, 63, 609, 627, 641, 1017
BspANI GGCC 4 cut(s) 612, 696, 732, 960
BspCNI CTCAG 1 cut(s) 664
BspEI TCCGGA 1 cut(s) 350
BspFNI CGCG 3 cut(s) 299, 546, 609
BspLI GGNNCC 4 cut(s) 6, 385, 573, 936
BspPI GGATC 4 cut(s) 116, 125, 187, 612
BspT107I GGYRCC 1 cut(s) 934
BsrBI CCGCTC 2 cut(s) 65, 1017
BsrDI GCAATG 1 cut(s) 246
BsrFI RCCGGY 2 cut(s) 475, 931
BsrI ACTGG 3 cut(s) 8, 573, 951
BssAI RCCGGY 2 cut(s) 475, 931
BssECI CCNNGG 4 cut(s) 24, 174, 259, 304
BssMI GATC 7 cut(s) 121, 130, 179, 187, 604, 688, 1045
BssNAI GTATAC 1 cut(s) 674
BssT1I CCWWGG 3 cut(s) 24, 259, 304
Bst1107I GTATAC 1 cut(s) 674
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 3 cut(s) 766, 785, 862
Bst6I CTCTTC 2 cut(s) 171, 324
BstC8I GCNNGC 4 cut(s) 51, 301, 481, 624
BstDEI CTNAG 1 cut(s) 651
BstDSI CCRYGG 3 cut(s) 24, 259, 304
BstF5I GGATG 4 cut(s) 34, 294, 448, 711
BstFNI CGCG 3 cut(s) 299, 546, 609
BstH2I RGCGCY 1 cut(s) 777
BstHHI GCGC 2 cut(s) 548, 776
BstKTI GATC 7 cut(s) 124, 133, 182, 190, 607, 691, 1048
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 7 cut(s) 121, 130, 179, 187, 604, 688, 1045
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 215
BstNSI RCATGY 2 cut(s) 373, 665
BstSCI CCNGG 1 cut(s) 213
BstSFI CTRYAG 1 cut(s) 150
BstUI CGCG 3 cut(s) 299, 546, 609
BstV1I GCAGC 1 cut(s) 763
BstX2I RGATCY 2 cut(s) 121, 179
BstYI RGATCY 2 cut(s) 121, 179
BstZ17I GTATAC 1 cut(s) 674
BsuRI GGCC 4 cut(s) 612, 696, 732, 960
BtgI CCRYGG 3 cut(s) 24, 259, 304
BtgZI GCGATG 1 cut(s) 608
BtsCI GGATG 4 cut(s) 34, 294, 448, 711
BtsIMutI CAGTG 3 cut(s) 493, 684, 775
Cac8I GCNNGC 4 cut(s) 51, 301, 481, 624
CfoI GCGC 2 cut(s) 548, 776
Cfr10I RCCGGY 2 cut(s) 475, 931
Cfr13I GGNCC 1 cut(s) 905
CseI GACGC 1 cut(s) 305
CsiI ACCWGGT 1 cut(s) 213
Csp6I GTAC 2 cut(s) 379, 446
CviQI GTAC 2 cut(s) 379, 446
DdeI CTNAG 1 cut(s) 651
DpnI GATC 7 cut(s) 123, 132, 181, 189, 606, 690, 1047
DpnII GATC 7 cut(s) 121, 130, 179, 187, 604, 688, 1045
Eam1104I CTCTTC 2 cut(s) 171, 324
EarI CTCTTC 2 cut(s) 171, 324
EciI GGCGGA 1 cut(s) 630
Eco130I CCWWGG 3 cut(s) 24, 259, 304
Eco24I GRGCYC 1 cut(s) 576
Eco32I GATATC 1 cut(s) 885
Eco47I GGWCC 1 cut(s) 905
Eco57I CTGAAG 1 cut(s) 469
Eco88I CYCGRG 2 cut(s) 175, 452
EcoRII CCWGG 1 cut(s) 213
EcoRV GATATC 1 cut(s) 885
EcoT14I CCWWGG 3 cut(s) 24, 259, 304
EcoT22I ATGCAT 1 cut(s) 852
EcoT38I GRGCYC 1 cut(s) 576
ErhI CCWWGG 3 cut(s) 24, 259, 304
FaqI GGGAC 3 cut(s) 251, 440, 579
FauI CCCGC 1 cut(s) 70
FbaI TGATCA 3 cut(s) 187, 688, 1045
FblI GTMKAC 1 cut(s) 673
Fnu4HI GCNGC 4 cut(s) 610, 627, 777, 1018
FokI GGATG 4 cut(s) 41, 301, 435, 698
FriOI GRGCYC 1 cut(s) 576
Fsp4HI GCNGC 4 cut(s) 610, 627, 777, 1018
FspBI CTAG 1 cut(s) 1055
GlaI GCGC 2 cut(s) 547, 775
GluI GCNGC 4 cut(s) 610, 627, 777, 1018
GsuI CTGGAG 2 cut(s) 442, 590
HaeII RGCGCY 1 cut(s) 777
HaeIII GGCC 4 cut(s) 612, 696, 732, 960
HapII CCGG 4 cut(s) 351, 476, 932, 938
HgaI GACGC 1 cut(s) 305
HhaI GCGC 2 cut(s) 548, 776
Hin6I GCGC 2 cut(s) 546, 774
HinP1I GCGC 2 cut(s) 546, 774
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HinfI GANTC 3 cut(s) 56, 264, 274
HpaII CCGG 4 cut(s) 351, 476, 932, 938
HphI GGTGA 5 cut(s) 51, 163, 451, 694, 754
Hpy166II GTNNAC 3 cut(s) 674, 796, 865
Hpy188I TCNGA 2 cut(s) 556, 969
Hpy188III TCNNGA 7 cut(s) 134, 177, 185, 351, 421, 452, 715
Hpy8I GTNNAC 3 cut(s) 674, 796, 865
HpyAV CCTTC 6 cut(s) 90, 103, 277, 328, 425, 950
HpyCH4III ACNGT 3 cut(s) 766, 785, 862
HpyCH4V TGCA 7 cut(s) 143, 228, 251, 401, 665, 814, 850
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 651
HspAI GCGC 2 cut(s) 546, 774
Kpn2I TCCGGA 1 cut(s) 350
Ksp22I TGATCA 3 cut(s) 187, 688, 1045
Kzo9I GATC 7 cut(s) 121, 130, 179, 187, 604, 688, 1045
LmnI GCTCC 4 cut(s) 10, 200, 571, 970
Lsp1109I GCAGC 1 cut(s) 763
LweI GCATC 4 cut(s) 48, 580, 837, 859
MabI ACCWGGT 1 cut(s) 213
MaeI CTAG 1 cut(s) 1055
MaeIII GTNAC 2 cut(s) 700, 942
MalI GATC 7 cut(s) 123, 132, 181, 189, 606, 690, 1047
MbiI CCGCTC 2 cut(s) 65, 1017
MboI GATC 7 cut(s) 121, 130, 179, 187, 604, 688, 1045
MboII GAAGA 4 cut(s) 158, 235, 341, 424
MfeI CAATTG 2 cut(s) 666, 912
MflI RGATCY 2 cut(s) 121, 179
MhlI GDGCHC 1 cut(s) 576
MluCI AATT 7 cut(s) 137, 222, 252, 666, 816, 912, 985
MluI ACGCGT 1 cut(s) 297
MlyI GAGTC 2 cut(s) 273, 283
MmeI TCCRAC 1 cut(s) 958
Mph1103I ATGCAT 1 cut(s) 852
MroI TCCGGA 1 cut(s) 350
MseI TTAA 1 cut(s) 255
MspI CCGG 4 cut(s) 351, 476, 932, 938
MspR9I CCNGG 1 cut(s) 215
MunI CAATTG 2 cut(s) 666, 912
MvaI CCWGG 1 cut(s) 215
MvnI CGCG 3 cut(s) 299, 546, 609
MwoI GCNNNNNNNGC 1 cut(s) 11
NcoI CCATGG 3 cut(s) 24, 259, 304
NdeII GATC 7 cut(s) 121, 130, 179, 187, 604, 688, 1045
NlaIV GGNNCC 4 cut(s) 6, 385, 573, 936
NmuCI GTSAC 1 cut(s) 700
NsiI ATGCAT 1 cut(s) 852
NspI RCATGY 2 cut(s) 373, 665
PciI ACATGT 1 cut(s) 369
PfeI GAWTC 1 cut(s) 56
PflFI GACNNNGTC 2 cut(s) 215, 410
PflMI CCANNNNNTGG 1 cut(s) 214
PkrI GCNGC 4 cut(s) 611, 628, 778, 1019
PleI GAGTC 2 cut(s) 272, 282
PpsI GAGTC 2 cut(s) 272, 282
PscI ACATGT 1 cut(s) 369
PsiI TTATAA 1 cut(s) 990
Psp6I CCWGG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 213
PspN4I GGNNCC 4 cut(s) 6, 385, 573, 936
PspPI GGNCC 1 cut(s) 905
PsuI RGATCY 2 cut(s) 121, 179
PsyI GACNNNGTC 2 cut(s) 215, 410
RsaI GTAC 2 cut(s) 380, 447
RsaNI GTAC 2 cut(s) 379, 446
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 4 cut(s) 610, 627, 777, 1018
Sau3AI GATC 7 cut(s) 121, 130, 179, 187, 604, 688, 1045
Sau96I GGNCC 1 cut(s) 905
SchI GAGTC 2 cut(s) 273, 283
ScrFI CCNGG 1 cut(s) 215
SduI GDGCHC 1 cut(s) 576
SexAI ACCWGGT 1 cut(s) 213
SfaNI GCATC 4 cut(s) 48, 580, 837, 859
SfcI CTRYAG 1 cut(s) 150
SinI GGWCC 1 cut(s) 905
Sse9I AATT 7 cut(s) 137, 222, 252, 666, 816, 912, 985
SsiI CCGC 6 cut(s) 53, 63, 609, 627, 641, 1017
SspMI CTAG 1 cut(s) 1055
StyD4I CCNGG 1 cut(s) 213
StyI CCWWGG 3 cut(s) 24, 259, 304
TaaI ACNGT 3 cut(s) 766, 785, 862
TasI AATT 7 cut(s) 137, 222, 252, 666, 816, 912, 985
TatI WGTACW 1 cut(s) 445
TauI GCSGC 3 cut(s) 612, 629, 1020
TfiI GAWTC 1 cut(s) 56
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 3 cut(s) 493, 691, 775
TseFI GTSAC 1 cut(s) 700
TseI GCWGC 1 cut(s) 776
Tsp45I GTSAC 1 cut(s) 700
TspDTI ATGAA 7 cut(s) 129, 235, 296, 611, 740, 998, 1046
TspGWI ACGGA 1 cut(s) 396
TspRI CASTG 3 cut(s) 493, 691, 775
Tth111I GACNNNGTC 2 cut(s) 215, 410
Van91I CCANNNNNTGG 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 905
XapI RAATTY 3 cut(s) 137, 222, 816
XceI RCATGY 2 cut(s) 373, 665
XmiI GTMKAC 1 cut(s) 673
XspI CTAG 1 cut(s) 1055
Zsp2I ATGCAT 1 cut(s) 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.