RchiOBHm_Chr5g0068661

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000481
Physical Location & Seq
Reverse (-)
74612224 .. 74613551
1328 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34409

Sequence Viewer

Length: 1089 bp
ATGGCGCCAGCAATAGCTCAAAATGTGGAGACATCGACTGTCACTCCAACCAAAGTGACTAGCATTAAAACACTTGCTGAGAGTTCAGCTGCTCTCAGCTCTGTACCTTCTGCATACGCCTTTACCATAAATCCCAACGATGAAGCTGATCCTAATGACCCCGAATTCGCAGTTCCAATTATCGATATGTCTCTTCTCACCTGCGGTTCACCTGATCAACGCTCCAAGATCATCCAAGACCTTGTCAAAATTTGTCAAGAATGGGGATTCTTCATAGTGATTAACCATGGAGTGCCAGAGAGCCTAATGAAGGCGATGATCGATTCATGTCATGGATTTTTCAGTCTTCCGGAGAGTGAGAAGCAGGAGTTTAAATCAGGAAACGATGTGCTGGAGATGTTCAAGTACGGGACTAGCTATAATCTTGCATTGGACAAAGTCCTTCTCTGGAGGGACTTCTTCAAGGTCAGAACACATCCCGAATTCTACTCCCTCAACAAACCGGCTAGCTTCAGTGAGATATCATTGGAGTTCAGCAAAAGAACAAGAGAAGTAGCATTGGAAATCATCAAAGCTATATCGGAAAGCCTGGGATTGGAGCCCAACTACATATATGATGCCATGAATATGGATCGGGGCTTACAAATGCTAGCCGGGAACTACTACCCTCTTTGCCCTCAGCCAGAACATGCAATTGGTATACCACATCATACAGATCATGGTCTAGTGACACTTCTCATTCAGAATGAGATGAAGGGTCTCCAAGTCGAACACAATGGGAAATGGCTCACTGTCGATGGCCCTGCAAACGGTTTTTTTGTTAATCTTGCTGACCAAATGCAGATTCTTACAAACGGAAAGTACAAGAGTGTGATGCACCGGGCAATTGTGAATAACAAAGCTGCAAGGATATCTATAGCCATACCACATGGACCATCTGTAGATACAACTATTGCACCTTCACCAGAATTGTGTGACAGAGAAGGTCAGGCTCCAAAATACCTTGCAATGAACTACAAGGAATACATACAACTTCAACAAAGTGGCAAGAATTACATGAAGTCAACCTTCGATCACATACGAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

362

Amino Acids

40.42

Weight (kDa)

5.68

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 58 - 161 2.9e-26 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 215 - 309 3.2e-27 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 209
Acc36I ACCTGC 1 cut(s) 209
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 700
AccIII TCCGGA 1 cut(s) 349
AciI CCGC 1 cut(s) 204
AclWI GGATC 2 cut(s) 143, 639
AcsI RAATTY 3 cut(s) 164, 249, 482
AcuI CTGAAG 1 cut(s) 496
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 105, 407, 863
AfiI CCNNNNNNNGG 3 cut(s) 310, 595, 809
AgsI TTSAA 3 cut(s) 403, 463, 1037
AjnI CCWGG 1 cut(s) 588
AjuI GAANNNNNNNTTGG 4 cut(s) 678, 710, 828, 860
AluBI AGCT 8 cut(s) 17, 89, 99, 146, 417, 510, 575, 902
AluI AGCT 8 cut(s) 17, 89, 99, 146, 417, 510, 575, 902
Alw26I GTCTC 3 cut(s) 23, 195, 764
AlwI GGATC 2 cut(s) 143, 639
Aor13HI TCCGGA 1 cut(s) 349
AoxI GGCC 1 cut(s) 799
ApeKI GCWGC 2 cut(s) 89, 902
ApoI RAATTY 3 cut(s) 164, 249, 482
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 800, 932
AsuC2I CCSGG 2 cut(s) 655, 881
AsuHPI GGTGA 3 cut(s) 190, 201, 954
AsuNHI GCTAGC 2 cut(s) 506, 649
AvaII GGWCC 1 cut(s) 932
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 603
BbsI GAAGAC 1 cut(s) 338
BbvCI CCTCAGC 1 cut(s) 678
BbvI GCAGC 2 cut(s) 76, 889
BccI CCATC 2 cut(s) 791, 943
BcgI CGANNNNNNTGC 2 cut(s) 785, 819
BciT130I CCWGG 1 cut(s) 590
BclI TGATCA 1 cut(s) 214
BcnI CCSGG 2 cut(s) 655, 881
BcoDI GTCTC 3 cut(s) 23, 195, 764
BfaI CTAG 6 cut(s) 60, 414, 507, 650, 725, 1087
BfmI CTRYAG 2 cut(s) 915, 939
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 209
BisI GCNGC 2 cut(s) 90, 903
BlsI GCNGC 2 cut(s) 91, 904
Bme1390I CCNGG 3 cut(s) 590, 655, 881
Bme18I GGWCC 1 cut(s) 932
BmgT120I GGNCC 2 cut(s) 800, 932
BmiI GGNNCC 3 cut(s) 6, 600, 993
BmrFI CCNGG 3 cut(s) 590, 655, 881
BmsI GCATC 2 cut(s) 607, 864
BmtI GCTAGC 2 cut(s) 510, 653
BpiI GAAGAC 1 cut(s) 338
BpmI CTGGAG 2 cut(s) 413, 469
Bpu10I CCTNAGC 1 cut(s) 678
BpuMI CCSGG 2 cut(s) 655, 881
Bsa29I ATCGAT 2 cut(s) 183, 321
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 764
BsaJI CCNNGG 2 cut(s) 286, 589
BsaWI WCCGGW 1 cut(s) 349
BsaXI ACNNNNNCTCC 2 cut(s) 28, 58
Bsc4I CCNNNNNNNGG 3 cut(s) 310, 595, 809
Bse118I RCCGGY 1 cut(s) 502
Bse3DI GCAATG 1 cut(s) 1014
BseAI TCCGGA 1 cut(s) 349
BseBI CCWGG 1 cut(s) 590
BseCI ATCGAT 2 cut(s) 183, 321
BseDI CCNNGG 2 cut(s) 286, 589
BseGI GGATG 2 cut(s) 231, 475
BseLI CCNNNNNNNGG 3 cut(s) 310, 595, 809
BseMI GCAATG 1 cut(s) 1014
BseMII CTCAG 3 cut(s) 69, 109, 692
BseXI GCAGC 2 cut(s) 76, 889
BshFI GGCC 1 cut(s) 801
BshNI GGYRCC 1 cut(s) 4
BshVI ATCGAT 2 cut(s) 183, 321
BsiSI CCGG 4 cut(s) 350, 503, 654, 880
BslFI GGGAC 2 cut(s) 424, 467
BslI CCNNNNNNNGG 3 cut(s) 310, 595, 809
BsmAI GTCTC 3 cut(s) 23, 195, 764
BsmFI GGGAC 2 cut(s) 424, 467
BsnI GGCC 1 cut(s) 801
Bso31I GGTCTC 1 cut(s) 764
Bsp1286I GDGCHC 1 cut(s) 603
Bsp13I TCCGGA 1 cut(s) 349
Bsp143I GATC 7 cut(s) 148, 214, 228, 318, 631, 715, 1072
Bsp19I CCATGG 1 cut(s) 286
BspACI CCGC 1 cut(s) 204
BspANI GGCC 1 cut(s) 801
BspCNI CTCAG 3 cut(s) 70, 108, 691
BspDI ATCGAT 2 cut(s) 183, 321
BspEI TCCGGA 1 cut(s) 349
BspLI GGNNCC 3 cut(s) 6, 600, 993
BspMI ACCTGC 1 cut(s) 209
BspOI GCTAGC 2 cut(s) 510, 653
BspPI GGATC 2 cut(s) 143, 639
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 764
BsrDI GCAATG 1 cut(s) 1014
BsrFI RCCGGY 1 cut(s) 502
BssAI RCCGGY 1 cut(s) 502
BssECI CCNNGG 2 cut(s) 286, 589
BssMI GATC 7 cut(s) 148, 214, 228, 318, 631, 715, 1072
BssNAI GTATAC 1 cut(s) 701
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 286
Bst1107I GTATAC 1 cut(s) 701
Bst2UI CCWGG 1 cut(s) 590
Bst4CI ACNGT 3 cut(s) 40, 793, 812
Bst6I CTCTTC 1 cut(s) 198
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 3 cut(s) 9, 508, 651
BstDEI CTNAG 3 cut(s) 78, 95, 678
BstDSI CCRYGG 1 cut(s) 286
BstENI CCTNNNNNAGG 1 cut(s) 308
BstF5I GGATG 2 cut(s) 231, 475
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 7 cut(s) 151, 217, 231, 321, 634, 718, 1075
BstMAI GTCTC 3 cut(s) 23, 195, 764
BstMBI GATC 7 cut(s) 148, 214, 228, 318, 631, 715, 1072
BstNI CCWGG 1 cut(s) 590
BstNSI RCATGY 1 cut(s) 692
BstSCI CCNGG 3 cut(s) 588, 653, 879
BstSFI CTRYAG 2 cut(s) 915, 939
BstV1I GCAGC 2 cut(s) 76, 889
BstV2I GAAGAC 1 cut(s) 338
BstXI CCANNNNNNTGG 1 cut(s) 628
BstZ17I GTATAC 1 cut(s) 701
Bsu15I ATCGAT 2 cut(s) 183, 321
BsuRI GGCC 1 cut(s) 801
BsuTUI ATCGAT 2 cut(s) 183, 321
BtgI CCRYGG 1 cut(s) 286
BtgZI GCGATG 1 cut(s) 329
BtsCI GGATG 2 cut(s) 231, 475
BtsIMutI CAGTG 2 cut(s) 520, 789
BveI ACCTGC 1 cut(s) 209
Cac8I GCNNGC 3 cut(s) 9, 508, 651
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 502
Cfr13I GGNCC 2 cut(s) 800, 932
ClaI ATCGAT 2 cut(s) 183, 321
Csp6I GTAC 3 cut(s) 104, 406, 862
CviAII CATG 8 cut(s) 287, 327, 332, 622, 689, 719, 929, 1057
CviQI GTAC 3 cut(s) 104, 406, 862
DdeI CTNAG 3 cut(s) 78, 95, 678
DinI GGCGCC 1 cut(s) 6
DpnI GATC 7 cut(s) 150, 216, 230, 320, 633, 717, 1074
DpnII GATC 7 cut(s) 148, 214, 228, 318, 631, 715, 1072
DraI TTTAAA 1 cut(s) 373
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco130I CCWWGG 1 cut(s) 286
Eco24I GRGCYC 1 cut(s) 603
Eco31I GGTCTC 1 cut(s) 764
Eco32I GATATC 2 cut(s) 522, 912
Eco47I GGWCC 1 cut(s) 932
Eco57I CTGAAG 1 cut(s) 496
EcoNI CCTNNNNNAGG 1 cut(s) 308
EcoRI GAATTC 2 cut(s) 164, 482
EcoRII CCWGG 1 cut(s) 588
EcoRV GATATC 2 cut(s) 522, 912
EcoT14I CCWWGG 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 603
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 286
FaeI CATG 8 cut(s) 290, 330, 335, 625, 692, 722, 932, 1060
FalI AAGNNNNNCTT 2 cut(s) 1052, 1084
FaqI GGGAC 2 cut(s) 424, 467
FatI CATG 8 cut(s) 286, 326, 331, 621, 688, 718, 928, 1056
FbaI TGATCA 1 cut(s) 214
FblI GTMKAC 1 cut(s) 700
Fnu4HI GCNGC 2 cut(s) 90, 903
FokI GGATG 2 cut(s) 218, 462
FriOI GRGCYC 1 cut(s) 603
Fsp4HI GCNGC 2 cut(s) 90, 903
FspBI CTAG 6 cut(s) 60, 414, 507, 650, 725, 1087
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 90, 903
GsuI CTGGAG 2 cut(s) 413, 469
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 801
HapII CCGG 4 cut(s) 350, 503, 654, 880
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 8 cut(s) 290, 330, 335, 625, 692, 722, 932, 1060
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 1065
HindII GTYRAC 1 cut(s) 1065
HinfI GANTC 4 cut(s) 267, 323, 844, 1083
HpaII CCGG 4 cut(s) 350, 503, 654, 880
HphI GGTGA 3 cut(s) 190, 201, 954
Hpy166II GTNNAC 3 cut(s) 209, 701, 1065
Hpy188I TCNGA 3 cut(s) 470, 583, 744
Hpy188III TCNNGA 5 cut(s) 257, 350, 378, 448, 479
Hpy8I GTNNAC 3 cut(s) 209, 701, 1065
HpyAV CCTTC 7 cut(s) 117, 304, 452, 748, 969, 977, 1078
HpyCH4III ACNGT 3 cut(s) 40, 793, 812
HpyCH4V TGCA 9 cut(s) 113, 428, 692, 806, 841, 877, 905, 956, 1007
HpyF3I CTNAG 3 cut(s) 78, 95, 678
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 8 cut(s) 290, 330, 335, 625, 692, 722, 932, 1060
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Kpn2I TCCGGA 1 cut(s) 349
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 7 cut(s) 148, 214, 228, 318, 631, 715, 1072
LmnI GCTCC 3 cut(s) 227, 598, 997
Lsp1109I GCAGC 2 cut(s) 76, 889
LweI GCATC 2 cut(s) 607, 864
MaeI CTAG 6 cut(s) 60, 414, 507, 650, 725, 1087
MaeIII GTNAC 4 cut(s) 40, 55, 727, 974
MalI GATC 7 cut(s) 150, 216, 230, 320, 633, 717, 1074
MboI GATC 7 cut(s) 148, 214, 228, 318, 631, 715, 1072
MboII GAAGA 4 cut(s) 185, 262, 338, 451
MfeI CAATTG 2 cut(s) 693, 885
MhlI GDGCHC 1 cut(s) 603
MluCI AATT 8 cut(s) 164, 177, 249, 482, 693, 885, 968, 1051
Mly113I GGCGCC 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 71
MnlI CCTC 4 cut(s) 444, 503, 678, 687
MroI TCCGGA 1 cut(s) 349
MseI TTAA 4 cut(s) 66, 282, 372, 822
MslI CAYNNNNRTG 1 cut(s) 626
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 4 cut(s) 350, 503, 654, 880
MspR9I CCNGG 3 cut(s) 590, 655, 881
MunI CAATTG 2 cut(s) 693, 885
MvaI CCWGG 1 cut(s) 590
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 2 cut(s) 655, 881
NcoI CCATGG 1 cut(s) 286
NdeII GATC 7 cut(s) 148, 214, 228, 318, 631, 715, 1072
NheI GCTAGC 2 cut(s) 506, 649
NlaIII CATG 8 cut(s) 290, 330, 335, 625, 692, 722, 932, 1060
NlaIV GGNNCC 3 cut(s) 6, 600, 993
NmuCI GTSAC 4 cut(s) 40, 55, 727, 974
NspI RCATGY 1 cut(s) 692
PaqCI CACCTGC 1 cut(s) 209
PfeI GAWTC 3 cut(s) 267, 323, 844
PflFI GACNNNGTC 2 cut(s) 242, 437
PkrI GCNGC 2 cut(s) 91, 904
PluTI GGCGCC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 588
PspGI CCWGG 1 cut(s) 588
PspN4I GGNNCC 3 cut(s) 6, 600, 993
PspPI GGNCC 2 cut(s) 800, 932
PsyI GACNNNGTC 2 cut(s) 242, 437
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 3 cut(s) 105, 407, 863
RsaNI GTAC 3 cut(s) 104, 406, 862
RseI CAYNNNNRTG 1 cut(s) 626
SaqAI TTAA 4 cut(s) 66, 282, 372, 822
SatI GCNGC 2 cut(s) 90, 903
Sau3AI GATC 7 cut(s) 148, 214, 228, 318, 631, 715, 1072
Sau96I GGNCC 2 cut(s) 800, 932
ScrFI CCNGG 3 cut(s) 590, 655, 881
SduI GDGCHC 1 cut(s) 603
SfaNI GCATC 2 cut(s) 607, 864
SfcI CTRYAG 2 cut(s) 915, 939
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 932
SmiMI CAYNNNNRTG 1 cut(s) 626
Sse9I AATT 8 cut(s) 164, 177, 249, 482, 693, 885, 968, 1051
SsiI CCGC 1 cut(s) 204
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 6 cut(s) 60, 414, 507, 650, 725, 1087
StyD4I CCNGG 3 cut(s) 588, 653, 879
StyI CCWWGG 1 cut(s) 286
TaaI ACNGT 3 cut(s) 40, 793, 812
TaqI TCGA 6 cut(s) 35, 183, 321, 768, 795, 1071
TasI AATT 8 cut(s) 164, 177, 249, 482, 693, 885, 968, 1051
TatI WGTACW 1 cut(s) 861
TfiI GAWTC 3 cut(s) 267, 323, 844
Tru1I TTAA 4 cut(s) 66, 282, 372, 822
Tru9I TTAA 4 cut(s) 66, 282, 372, 822
TscAI CASTG 2 cut(s) 520, 796
TseFI GTSAC 4 cut(s) 40, 55, 727, 974
TseI GCWGC 2 cut(s) 89, 902
Tsp45I GTSAC 4 cut(s) 40, 55, 727, 974
TspDTI ATGAA 8 cut(s) 156, 262, 315, 323, 638, 767, 1025, 1073
TspGWI ACGGA 1 cut(s) 870
TspRI CASTG 2 cut(s) 520, 796
Tth111I GACNNNGTC 2 cut(s) 242, 437
VpaK11BI GGWCC 1 cut(s) 932
XagI CCTNNNNNAGG 1 cut(s) 308
XapI RAATTY 3 cut(s) 164, 249, 482
XceI RCATGY 1 cut(s) 692
XmiI GTMKAC 1 cut(s) 700
XspI CTAG 6 cut(s) 60, 414, 507, 650, 725, 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.