Rw7G019340

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000481
Physical Location & Seq
Reverse (-)
21421657 .. 21423168
1512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G019340.1

Sequence Viewer

Length: 1068 bp
ATGGCTGCAGTTGCTCAAGCCCAGGAGACATCAAATGTCACAAGCATCAAATCACTAGCTGAGTCACCAGCTCTCAACTCTGTGCCTTCTGCATATGCCTTCAACATAAACCCCAATGATGAAGCTGACCCTAATGACCCTGAGTTTGCTATTCCCATTGTTGATATGTCTCTTCTCACCTCTGGTTCTCCTGACGACCGCGCAAAAATCGTTGGCGATCTTGTCAAAATTTGTGAAGAATGGGGCTTCTTCATTGCAATTAACCACGGAGTACCAGAGAACCTAATGAAGGCGATGATTGACGCTTGTCATGGATTTTTCCGTCTTCCAGACGAGGAGAAGAGGGAGTTTAAATCAGGAAATGATGTGCTTGAGATGTTCAAGTATGGCACGAGCTATAATCTTGCGTTGGACAAAGTACTTCTATGGAGAGACTTCTTCAAGGTCCGAACACATCCCGAGTTCTACTCCCTCTACAAACCGGCTAGCTTCAGTGAAATTTCGCTGGAGTTTAGCAAAAGAAGCCGAGAAGTTGCCTTAGAAATAACGAGAGCAATATCGGAAAGCTTGGGTTTGGGGCCGGACTACATATACAACACAATGAACATGGATCGCGGCTTACAAATGCTAGCGGGGAACTACTACCCACCTTGTCCTCAGCCTGAACATGCCATTGGTATACCCCATCATACCGATCATGGTCTAGTCACTCTCCTCATCCAAAATGAAATGAATGGCCTCCAAGTCGAGCACAATGGAAAATGGCTCACTGTCAATGGCCCTAAGAATGGCTTTTTCGTTAACCTTGCGGATCAAATGCAGATTCTTACAAATGGCAAGTACAAGAGTGTGATGCATCGCGCCACCGTGAACAACAAAGCTACAAGAATATCGATAGCCATACCACATGGACCGTCTGTGGATACAATTATCGCACCAGCACCAGAGTTGTGTGAAAGAGAAGGCCAAGCTCCAAAGTACCTTGCAATGAACTACAAGGAATACATACAACTTCAGCAAAGCGGCAAGAACTATATGAAGTCCACATTTGATCACATCCGAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

355

Amino Acids

39.76

Weight (kDa)

5.68

Isoelectric Point (pI)

36.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 51 - 155 1e-24 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 208 - 302 1.6e-29 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 679
AccII CGCG 3 cut(s) 201, 615, 861
AciI CCGC 5 cut(s) 199, 615, 632, 809, 1023
AclWI GGATC 2 cut(s) 618, 819
AcsI RAATTY 2 cut(s) 228, 498
AcuI CTGAAG 2 cut(s) 475, 998
AfaI GTAC 4 cut(s) 273, 420, 842, 980
AfiI CCNNNNNNNGG 2 cut(s) 289, 788
AgsI TTSAA 3 cut(s) 103, 382, 442
AjnI CCWGG 1 cut(s) 21
AjuI GAANNNNNNNTTGG 2 cut(s) 657, 689
AluBI AGCT 8 cut(s) 59, 71, 125, 396, 489, 567, 881, 971
AluI AGCT 8 cut(s) 59, 71, 125, 396, 489, 567, 881, 971
Alw21I GWGCWC 1 cut(s) 753
Alw26I GTCTC 3 cut(s) 20, 174, 426
AlwI GGATC 2 cut(s) 618, 819
Ama87I CYCGRG 1 cut(s) 458
AoxI GGCC 4 cut(s) 578, 736, 778, 964
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 2 cut(s) 228, 498
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AspLEI GCGC 2 cut(s) 203, 863
AspS9I GGNCC 4 cut(s) 445, 578, 779, 911
AsuHPI GGTGA 2 cut(s) 57, 169
AsuNHI GCTAGC 2 cut(s) 485, 628
AvaI CYCGRG 1 cut(s) 458
AvaII GGWCC 2 cut(s) 445, 911
BauI CACGAG 1 cut(s) 391
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 1 cut(s) 753
BbvCI CCTCAGC 1 cut(s) 657
BccI CCATC 1 cut(s) 693
BciT130I CCWGG 1 cut(s) 23
BciVI GTATCC 1 cut(s) 916
BclI TGATCA 1 cut(s) 1051
BcoDI GTCTC 3 cut(s) 20, 174, 426
BfaI CTAG 5 cut(s) 56, 486, 629, 704, 1066
BfmI CTRYAG 1 cut(s) 6
BfuI GTATCC 1 cut(s) 916
BisI GCNGC 3 cut(s) 6, 616, 1024
BlsI GCNGC 3 cut(s) 7, 617, 1025
BmcAI AGTACT 1 cut(s) 420
Bme1390I CCNGG 1 cut(s) 23
Bme18I GGWCC 2 cut(s) 445, 911
BmeT110I CYCGRG 1 cut(s) 458
BmgT120I GGNCC 4 cut(s) 445, 578, 779, 911
BmiI GGNNCC 1 cut(s) 579
BmrFI CCNGG 1 cut(s) 23
BmsI GCATC 3 cut(s) 54, 843, 865
BmtI GCTAGC 2 cut(s) 489, 632
BoxI GACNNNNGTC 1 cut(s) 306
BpiI GAAGAC 1 cut(s) 317
BplI GAGNNNNNCTC 2 cut(s) 452, 484
BpmI CTGGAG 1 cut(s) 527
Bpu10I CCTNAGC 1 cut(s) 657
BpuEI CTTGAG 1 cut(s) 392
Bsa29I ATCGAT 1 cut(s) 893
BsaJI CCNNGG 2 cut(s) 21, 265
Bsc4I CCNNNNNNNGG 2 cut(s) 289, 788
Bse118I RCCGGY 1 cut(s) 481
Bse3DI GCAATG 2 cut(s) 252, 993
BseBI CCWGG 1 cut(s) 23
BseCI ATCGAT 1 cut(s) 893
BseDI CCNNGG 2 cut(s) 21, 265
BseGI GGATG 3 cut(s) 454, 717, 1056
BseLI CCNNNNNNNGG 2 cut(s) 289, 788
BseMI GCAATG 2 cut(s) 252, 993
BseMII CTCAG 3 cut(s) 51, 132, 671
BseRI GAGGAG 2 cut(s) 350, 704
Bsh1236I CGCG 3 cut(s) 201, 615, 861
Bsh1285I CGRYCG 1 cut(s) 199
BshFI GGCC 4 cut(s) 580, 738, 780, 966
BshVI ATCGAT 1 cut(s) 893
BsiEI CGRYCG 1 cut(s) 199
BsiHKAI GWGCWC 1 cut(s) 753
BsiHKCI CYCGRG 1 cut(s) 458
BsiSI CCGG 2 cut(s) 482, 581
BslI CCNNNNNNNGG 2 cut(s) 289, 788
BsmAI GTCTC 3 cut(s) 20, 174, 426
BsnI GGCC 4 cut(s) 580, 738, 780, 966
BsoBI CYCGRG 1 cut(s) 458
Bsp1286I GDGCHC 1 cut(s) 753
Bsp143I GATC 5 cut(s) 217, 610, 694, 811, 1051
BspACI CCGC 5 cut(s) 199, 615, 632, 809, 1023
BspANI GGCC 4 cut(s) 580, 738, 780, 966
BspCNI CTCAG 3 cut(s) 52, 133, 670
BspDI ATCGAT 1 cut(s) 893
BspFNI CGCG 3 cut(s) 201, 615, 861
BspLI GGNNCC 1 cut(s) 579
BspMAI CTGCAG 1 cut(s) 10
BspOI GCTAGC 2 cut(s) 489, 632
BspPI GGATC 2 cut(s) 618, 819
BsrDI GCAATG 2 cut(s) 252, 993
BsrFI RCCGGY 1 cut(s) 481
BssAI RCCGGY 1 cut(s) 481
BssECI CCNNGG 2 cut(s) 21, 265
BssMI GATC 5 cut(s) 217, 610, 694, 811, 1051
BssNAI GTATAC 1 cut(s) 680
BssSI CACGAG 1 cut(s) 391
Bst1107I GTATAC 1 cut(s) 680
Bst2BI CACGAG 1 cut(s) 391
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 3 cut(s) 772, 868, 915
Bst6I CTCTTC 2 cut(s) 177, 335
BstC8I GCNNGC 2 cut(s) 487, 630
BstDEI CTNAG 5 cut(s) 60, 141, 538, 657, 783
BstDSI CCRYGG 1 cut(s) 265
BstENI CCTNNNNNAGG 1 cut(s) 287
BstF5I GGATG 3 cut(s) 454, 717, 1056
BstFNI CGCG 3 cut(s) 201, 615, 861
BstHHI GCGC 2 cut(s) 203, 863
BstKTI GATC 5 cut(s) 220, 613, 697, 814, 1054
BstMAI GTCTC 3 cut(s) 20, 174, 426
BstMBI GATC 5 cut(s) 217, 610, 694, 811, 1051
BstMCI CGRYCG 1 cut(s) 199
BstMWI GCNNNNNNNGC 2 cut(s) 11, 522
BstNI CCWGG 1 cut(s) 23
BstNSI RCATGY 1 cut(s) 671
BstPAI GACNNNNGTC 1 cut(s) 306
BstSCI CCNGG 1 cut(s) 21
BstSFI CTRYAG 1 cut(s) 6
BstUI CGCG 3 cut(s) 201, 615, 861
BstV2I GAAGAC 1 cut(s) 317
BstZ17I GTATAC 1 cut(s) 680
Bsu15I ATCGAT 1 cut(s) 893
BsuI GTATCC 1 cut(s) 916
BsuRI GGCC 4 cut(s) 580, 738, 780, 966
BsuTUI ATCGAT 1 cut(s) 893
BtgI CCRYGG 1 cut(s) 265
BtgZI GCGATG 2 cut(s) 308, 842
BtsCI GGATG 3 cut(s) 454, 717, 1056
BtsIMutI CAGTG 2 cut(s) 499, 768
Cac8I GCNNGC 2 cut(s) 487, 630
CfoI GCGC 2 cut(s) 203, 863
Cfr10I RCCGGY 1 cut(s) 481
Cfr13I GGNCC 4 cut(s) 445, 578, 779, 911
ClaI ATCGAT 1 cut(s) 893
CseI GACGC 1 cut(s) 311
Csp6I GTAC 4 cut(s) 272, 419, 841, 979
CviAII CATG 5 cut(s) 311, 607, 668, 698, 908
CviQI GTAC 4 cut(s) 272, 419, 841, 979
DdeI CTNAG 5 cut(s) 60, 141, 538, 657, 783
DpnI GATC 5 cut(s) 219, 612, 696, 813, 1053
DpnII GATC 5 cut(s) 217, 610, 694, 811, 1051
DraI TTTAAA 1 cut(s) 352
Eam1104I CTCTTC 2 cut(s) 177, 335
EarI CTCTTC 2 cut(s) 177, 335
Eco47I GGWCC 2 cut(s) 445, 911
Eco57I CTGAAG 2 cut(s) 475, 998
Eco88I CYCGRG 1 cut(s) 458
EcoNI CCTNNNNNAGG 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 21
EcoT22I ATGCAT 1 cut(s) 858
FaeI CATG 5 cut(s) 314, 610, 671, 701, 911
FalI AAGNNNNNCTT 2 cut(s) 776, 808
FatI CATG 5 cut(s) 310, 606, 667, 697, 907
FauI CCCGC 1 cut(s) 625
FauNDI CATATG 1 cut(s) 94
FbaI TGATCA 1 cut(s) 1051
FblI GTMKAC 1 cut(s) 679
Fnu4HI GCNGC 3 cut(s) 6, 616, 1024
FokI GGATG 3 cut(s) 441, 704, 1043
Fsp4HI GCNGC 3 cut(s) 6, 616, 1024
FspBI CTAG 5 cut(s) 56, 486, 629, 704, 1066
GlaI GCGC 2 cut(s) 202, 862
GluI GCNGC 3 cut(s) 6, 616, 1024
GsuI CTGGAG 1 cut(s) 527
HaeIII GGCC 4 cut(s) 580, 738, 780, 966
HapII CCGG 2 cut(s) 482, 581
HgaI GACGC 1 cut(s) 311
HhaI GCGC 2 cut(s) 203, 863
Hin1II CATG 5 cut(s) 314, 610, 671, 701, 911
Hin6I GCGC 2 cut(s) 201, 861
HinP1I GCGC 2 cut(s) 201, 861
HincII GTYRAC 1 cut(s) 802
HindII GTYRAC 1 cut(s) 802
HindIII AAGCTT 1 cut(s) 565
HinfI GANTC 2 cut(s) 62, 823
HpaI GTTAAC 1 cut(s) 802
HpaII CCGG 2 cut(s) 482, 581
HphI GGTGA 2 cut(s) 57, 169
Hpy166II GTNNAC 4 cut(s) 680, 802, 871, 1044
Hpy188I TCNGA 3 cut(s) 449, 562, 1061
Hpy188III TCNNGA 4 cut(s) 191, 329, 357, 458
Hpy8I GTNNAC 4 cut(s) 680, 802, 871, 1044
HpyAV CCTTC 4 cut(s) 96, 109, 283, 956
HpyCH4III ACNGT 3 cut(s) 772, 868, 915
HpyCH4V TGCA 6 cut(s) 8, 92, 257, 820, 856, 986
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 522
HpyF3I CTNAG 5 cut(s) 60, 141, 538, 657, 783
Hsp92II CATG 5 cut(s) 314, 610, 671, 701, 911
HspAI GCGC 2 cut(s) 201, 861
Ksp22I TGATCA 1 cut(s) 1051
KspAI GTTAAC 1 cut(s) 802
Kzo9I GATC 5 cut(s) 217, 610, 694, 811, 1051
LmnI GCTCC 1 cut(s) 976
LweI GCATC 3 cut(s) 54, 843, 865
MaeI CTAG 5 cut(s) 56, 486, 629, 704, 1066
MaeIII GTNAC 3 cut(s) 37, 63, 706
MalI GATC 5 cut(s) 219, 612, 696, 813, 1053
MboI GATC 5 cut(s) 217, 610, 694, 811, 1051
MboII GAAGA 6 cut(s) 164, 241, 248, 317, 352, 430
MhlI GDGCHC 1 cut(s) 753
MluCI AATT 4 cut(s) 228, 258, 498, 927
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 1 cut(s) 390
MnlI CCTC 7 cut(s) 190, 328, 336, 482, 666, 725, 749
Mph1103I ATGCAT 1 cut(s) 858
MseI TTAA 3 cut(s) 261, 351, 801
MspI CCGG 2 cut(s) 482, 581
MspR9I CCNGG 1 cut(s) 23
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 3 cut(s) 201, 615, 861
MwoI GCNNNNNNNGC 2 cut(s) 11, 522
NdeI CATATG 1 cut(s) 94
NdeII GATC 5 cut(s) 217, 610, 694, 811, 1051
NheI GCTAGC 2 cut(s) 485, 628
NlaIII CATG 5 cut(s) 314, 610, 671, 701, 911
NlaIV GGNNCC 1 cut(s) 579
NmeAIII GCCGAG 1 cut(s) 551
NmuCI GTSAC 3 cut(s) 37, 63, 706
NsiI ATGCAT 1 cut(s) 858
NspI RCATGY 1 cut(s) 671
PfeI GAWTC 1 cut(s) 823
PkrI GCNGC 3 cut(s) 7, 617, 1025
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PshAI GACNNNNGTC 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 1 cut(s) 579
PspPI GGNCC 4 cut(s) 445, 578, 779, 911
PstI CTGCAG 1 cut(s) 10
RsaI GTAC 4 cut(s) 273, 420, 842, 980
RsaNI GTAC 4 cut(s) 272, 419, 841, 979
SaqAI TTAA 3 cut(s) 261, 351, 801
SatI GCNGC 3 cut(s) 6, 616, 1024
Sau3AI GATC 5 cut(s) 217, 610, 694, 811, 1051
Sau96I GGNCC 4 cut(s) 445, 578, 779, 911
ScaI AGTACT 1 cut(s) 420
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 1 cut(s) 23
SduI GDGCHC 1 cut(s) 753
SfaNI GCATC 3 cut(s) 54, 843, 865
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 2 cut(s) 445, 911
SmlI CTYRAG 2 cut(s) 15, 371
SmoI CTYRAG 2 cut(s) 15, 371
Sse9I AATT 4 cut(s) 228, 258, 498, 927
SsiI CCGC 5 cut(s) 199, 615, 632, 809, 1023
SspMI CTAG 5 cut(s) 56, 486, 629, 704, 1066
StyD4I CCNGG 1 cut(s) 21
TaaI ACNGT 3 cut(s) 772, 868, 915
TaqI TCGA 2 cut(s) 747, 893
TasI AATT 4 cut(s) 228, 258, 498, 927
TatI WGTACW 2 cut(s) 418, 840
TauI GCSGC 2 cut(s) 618, 1026
TfiI GAWTC 1 cut(s) 823
Tru1I TTAA 3 cut(s) 261, 351, 801
Tru9I TTAA 3 cut(s) 261, 351, 801
TscAI CASTG 2 cut(s) 499, 775
TseFI GTSAC 3 cut(s) 37, 63, 706
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 3 cut(s) 37, 63, 706
TspDTI ATGAA 8 cut(s) 135, 241, 302, 617, 741, 746, 1004, 1052
TspGWI ACGGA 2 cut(s) 282, 311
TspRI CASTG 2 cut(s) 499, 775
VpaK11BI GGWCC 2 cut(s) 445, 911
XagI CCTNNNNNAGG 1 cut(s) 287
XapI RAATTY 2 cut(s) 228, 498
XceI RCATGY 1 cut(s) 671
XmiI GTMKAC 1 cut(s) 679
XspI CTAG 5 cut(s) 56, 486, 629, 704, 1066
ZrmI AGTACT 1 cut(s) 420
Zsp2I ATGCAT 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.