Rw7G019330

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000481
Physical Location & Seq
Reverse (-)
21406536 .. 21408411
1876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G019330.1

Sequence Viewer

Length: 942 bp
ATGGCTGCAGTTGCTCAAGCCCAGGAGACATCAAATGTCACAAGCATCAAATCACTAGCTGAGTCATCAGCCCTCAACTCTGTGCCTTCTGCATATGCCTTCAACATAAACCCCCATGATGAAGCTGATCCTAATGACCCTGAGTTTGCTATTCCCATTGTTGATATGTCTCTTCTCACCTCTGGTTCTCCTGAAGAGCGCGCAAAAATCGTTGGCGATCTTGTCAAAATTTGTGAAGAATGGGGCTTCTTCATTGCAATTAACCATGGAGTACCAGAGAGCCTAATGAAGGCAATGATTGACATTCTATTCAAGTATTATAAGCTATACTTCTTCAACAAAGTACTTTTATGGAGGGACTTCTTCAAGGTCCGAACACACCCGGAGTTTCACTCCCTCTACAAACCAGCTAGCTTCAGTGAGGTTTCGCTGGAGTTTAGCAAAAGAAGCCGAGAAGTAGCCTTGGAAATAACGAGAGCAATATCGGAAAGCTTGGGTTTGGGGCCGGACTACATATACAACACAATGAACATGGATCGCGGCTTACAAATGCTAGCGGGGAACTACTACCCACCTTGTCCTCAGCCTGAACATGCCATTGGTATACCCCATCATACTGATCATGGTCTAGTCACACTCCTCATTCAGAATGAGATGAATGGTCTCCAAGTTGAGCACAATGGAAAATGGCTCACTGTCAATGGCCCTCCCAATGGCTTTTTCGTCAACCTTGCTGATCAAATGCAGATTCTTACAAATGGTAAGTACAAGAGTGTGATGCATCGGGCCACAGTGAACAACAAAGCTACAAGGATATCGATATTGTGTGAAAGAGAAGGCCAAGCTCCAAAGTACCTTGCTATGAACTACAAGGAATACATACAACTTCAGCAAAGCGGCAAGAACTACATGAAGTCTACATTTGATCATATCCGAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

313

Amino Acids

35.4

Weight (kDa)

6.26

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 51 - 109 4.2e-12 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 183 - 275 2.9e-29 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 321
AccI GTMKAC 2 cut(s) 604, 917
AccII CGCG 2 cut(s) 201, 540
AciI CCGC 3 cut(s) 540, 557, 897
AclWI GGATC 2 cut(s) 122, 543
AcsI RAATTY 1 cut(s) 228
AcuI CTGAAG 3 cut(s) 213, 400, 872
AfaI GTAC 4 cut(s) 273, 345, 767, 854
AfiI CCNNNNNNNGG 2 cut(s) 289, 713
AgsI TTSAA 4 cut(s) 103, 313, 337, 367
AjnI CCWGG 1 cut(s) 21
AjuI GAANNNNNNNTTGG 4 cut(s) 582, 614, 704, 736
AluBI AGCT 8 cut(s) 59, 125, 325, 410, 414, 492, 806, 845
AluI AGCT 8 cut(s) 59, 125, 325, 410, 414, 492, 806, 845
Alw21I GWGCWC 1 cut(s) 678
Alw26I GTCTC 3 cut(s) 20, 174, 668
AlwI GGATC 2 cut(s) 122, 543
AoxI GGCC 4 cut(s) 503, 703, 786, 838
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 228
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AspLEI GCGC 2 cut(s) 201, 203
AspS9I GGNCC 4 cut(s) 370, 503, 704, 786
AsuC2I CCSGG 1 cut(s) 383
AsuHPI GGTGA 1 cut(s) 169
AsuNHI GCTAGC 2 cut(s) 410, 553
AvaII GGWCC 1 cut(s) 370
Bbv12I GWGCWC 1 cut(s) 678
BbvCI CCTCAGC 1 cut(s) 582
BccI CCATC 1 cut(s) 618
BciT130I CCWGG 1 cut(s) 23
BclI TGATCA 3 cut(s) 619, 736, 925
BcnI CCSGG 1 cut(s) 383
BcoDI GTCTC 3 cut(s) 20, 174, 668
BfaI CTAG 5 cut(s) 56, 411, 554, 629, 940
BfmI CTRYAG 1 cut(s) 6
BisI GCNGC 3 cut(s) 6, 541, 898
BlsI GCNGC 3 cut(s) 7, 542, 899
BmcAI AGTACT 1 cut(s) 345
Bme1390I CCNGG 2 cut(s) 23, 383
Bme18I GGWCC 1 cut(s) 370
BmgT120I GGNCC 4 cut(s) 370, 503, 704, 786
BmiI GGNNCC 1 cut(s) 504
BmrFI CCNGG 2 cut(s) 23, 383
BmsI GCATC 3 cut(s) 54, 768, 790
BmtI GCTAGC 2 cut(s) 414, 557
BplI GAGNNNNNCTC 2 cut(s) 377, 409
BpmI CTGGAG 1 cut(s) 452
Bpu10I CCTNAGC 1 cut(s) 582
BpuMI CCSGG 1 cut(s) 383
Bsa29I ATCGAT 1 cut(s) 818
BsaI GGTCTC 1 cut(s) 668
BsaJI CCNNGG 3 cut(s) 21, 265, 462
Bsc4I CCNNNNNNNGG 2 cut(s) 289, 713
Bse3DI GCAATG 2 cut(s) 252, 300
BseBI CCWGG 1 cut(s) 23
BseCI ATCGAT 1 cut(s) 818
BseDI CCNNGG 3 cut(s) 21, 265, 462
BseLI CCNNNNNNNGG 2 cut(s) 289, 713
BseMI GCAATG 2 cut(s) 252, 300
BseMII CTCAG 3 cut(s) 51, 132, 596
BsePI GCGCGC 1 cut(s) 199
BseRI GAGGAG 1 cut(s) 629
Bsh1236I CGCG 2 cut(s) 201, 540
BshFI GGCC 4 cut(s) 505, 705, 788, 840
BshVI ATCGAT 1 cut(s) 818
BsiHKAI GWGCWC 1 cut(s) 678
BsiSI CCGG 2 cut(s) 383, 506
BslFI GGGAC 1 cut(s) 371
BslI CCNNNNNNNGG 2 cut(s) 289, 713
BsmAI GTCTC 3 cut(s) 20, 174, 668
BsmFI GGGAC 1 cut(s) 371
BsnI GGCC 4 cut(s) 505, 705, 788, 840
Bso31I GGTCTC 1 cut(s) 668
Bsp1286I GDGCHC 1 cut(s) 678
Bsp143I GATC 6 cut(s) 127, 217, 535, 619, 736, 925
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 3 cut(s) 540, 557, 897
BspANI GGCC 4 cut(s) 505, 705, 788, 840
BspCNI CTCAG 3 cut(s) 52, 133, 595
BspDI ATCGAT 1 cut(s) 818
BspFNI CGCG 2 cut(s) 201, 540
BspLI GGNNCC 1 cut(s) 504
BspMAI CTGCAG 1 cut(s) 10
BspOI GCTAGC 2 cut(s) 414, 557
BspPI GGATC 2 cut(s) 122, 543
BspQI GCTCTTC 1 cut(s) 189
BspTNI GGTCTC 1 cut(s) 668
BsrDI GCAATG 2 cut(s) 252, 300
BssECI CCNNGG 3 cut(s) 21, 265, 462
BssHII GCGCGC 1 cut(s) 199
BssMI GATC 6 cut(s) 127, 217, 535, 619, 736, 925
BssNAI GTATAC 1 cut(s) 605
BssT1I CCWWGG 2 cut(s) 265, 462
Bst1107I GTATAC 1 cut(s) 605
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 2 cut(s) 697, 793
Bst6I CTCTTC 2 cut(s) 177, 189
BstC8I GCNNGC 3 cut(s) 201, 412, 555
BstDEI CTNAG 3 cut(s) 60, 141, 582
BstDSI CCRYGG 1 cut(s) 265
BstENI CCTNNNNNAGG 1 cut(s) 287
BstFNI CGCG 2 cut(s) 201, 540
BstHHI GCGC 2 cut(s) 201, 203
BstKTI GATC 6 cut(s) 130, 220, 538, 622, 739, 928
BstMAI GTCTC 3 cut(s) 20, 174, 668
BstMBI GATC 6 cut(s) 127, 217, 535, 619, 736, 925
BstMWI GCNNNNNNNGC 2 cut(s) 11, 447
BstNI CCWGG 1 cut(s) 23
BstNSI RCATGY 1 cut(s) 596
BstSCI CCNGG 2 cut(s) 21, 381
BstSFI CTRYAG 1 cut(s) 6
BstUI CGCG 2 cut(s) 201, 540
BstZ17I GTATAC 1 cut(s) 605
Bsu15I ATCGAT 1 cut(s) 818
BsuRI GGCC 4 cut(s) 505, 705, 788, 840
BsuTUI ATCGAT 1 cut(s) 818
BtgI CCRYGG 1 cut(s) 265
BtsIMutI CAGTG 3 cut(s) 424, 693, 798
Cac8I GCNNGC 3 cut(s) 201, 412, 555
CfoI GCGC 2 cut(s) 201, 203
Cfr13I GGNCC 4 cut(s) 370, 503, 704, 786
ClaI ATCGAT 1 cut(s) 818
Csp6I GTAC 4 cut(s) 272, 344, 766, 853
CviAII CATG 6 cut(s) 116, 266, 532, 593, 623, 910
CviQI GTAC 4 cut(s) 272, 344, 766, 853
DdeI CTNAG 3 cut(s) 60, 141, 582
DpnI GATC 6 cut(s) 129, 219, 537, 621, 738, 927
DpnII GATC 6 cut(s) 127, 217, 535, 619, 736, 925
Eam1104I CTCTTC 2 cut(s) 177, 189
EarI CTCTTC 2 cut(s) 177, 189
Eco130I CCWWGG 2 cut(s) 265, 462
Eco31I GGTCTC 1 cut(s) 668
Eco32I GATATC 1 cut(s) 816
Eco47I GGWCC 1 cut(s) 370
Eco57I CTGAAG 3 cut(s) 213, 400, 872
EcoNI CCTNNNNNAGG 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 21
EcoRV GATATC 1 cut(s) 816
EcoT14I CCWWGG 2 cut(s) 265, 462
EcoT22I ATGCAT 1 cut(s) 783
ErhI CCWWGG 2 cut(s) 265, 462
FaeI CATG 6 cut(s) 119, 269, 535, 596, 626, 913
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FaqI GGGAC 1 cut(s) 371
FatI CATG 6 cut(s) 115, 265, 531, 592, 622, 909
FauI CCCGC 1 cut(s) 550
FauNDI CATATG 1 cut(s) 94
FbaI TGATCA 3 cut(s) 619, 736, 925
FblI GTMKAC 2 cut(s) 604, 917
Fnu4HI GCNGC 3 cut(s) 6, 541, 898
Fsp4HI GCNGC 3 cut(s) 6, 541, 898
FspBI CTAG 5 cut(s) 56, 411, 554, 629, 940
GlaI GCGC 2 cut(s) 200, 202
GluI GCNGC 3 cut(s) 6, 541, 898
GsuI CTGGAG 1 cut(s) 452
HaeIII GGCC 4 cut(s) 505, 705, 788, 840
HapII CCGG 2 cut(s) 383, 506
HhaI GCGC 2 cut(s) 201, 203
Hin1II CATG 6 cut(s) 119, 269, 535, 596, 626, 913
Hin6I GCGC 2 cut(s) 199, 201
HinP1I GCGC 2 cut(s) 199, 201
HincII GTYRAC 1 cut(s) 727
HindII GTYRAC 1 cut(s) 727
HindIII AAGCTT 1 cut(s) 490
HinfI GANTC 2 cut(s) 62, 748
HpaII CCGG 2 cut(s) 383, 506
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 4 cut(s) 605, 727, 796, 918
Hpy188I TCNGA 4 cut(s) 374, 487, 648, 935
Hpy188III TCNNGA 1 cut(s) 191
Hpy8I GTNNAC 4 cut(s) 605, 727, 796, 918
HpyAV CCTTC 4 cut(s) 96, 109, 283, 830
HpyCH4III ACNGT 2 cut(s) 697, 793
HpyCH4V TGCA 5 cut(s) 8, 92, 257, 745, 781
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 447
HpyF3I CTNAG 3 cut(s) 60, 141, 582
Hsp92II CATG 6 cut(s) 119, 269, 535, 596, 626, 913
HspAI GCGC 2 cut(s) 199, 201
Ksp22I TGATCA 3 cut(s) 619, 736, 925
Kzo9I GATC 6 cut(s) 127, 217, 535, 619, 736, 925
LguI GCTCTTC 1 cut(s) 189
LmnI GCTCC 1 cut(s) 850
LweI GCATC 3 cut(s) 54, 768, 790
MaeI CTAG 5 cut(s) 56, 411, 554, 629, 940
MaeIII GTNAC 2 cut(s) 37, 631
MalI GATC 6 cut(s) 129, 219, 537, 621, 738, 927
MboI GATC 6 cut(s) 127, 217, 535, 619, 736, 925
MboII GAAGA 6 cut(s) 164, 206, 241, 248, 325, 355
MhlI GDGCHC 1 cut(s) 678
MluCI AATT 2 cut(s) 228, 258
MlyI GAGTC 1 cut(s) 71
MnlI CCTC 8 cut(s) 83, 190, 348, 407, 415, 591, 650, 717
Mph1103I ATGCAT 1 cut(s) 783
MseI TTAA 1 cut(s) 261
MspI CCGG 2 cut(s) 383, 506
MspR9I CCNGG 2 cut(s) 23, 383
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 2 cut(s) 201, 540
MwoI GCNNNNNNNGC 2 cut(s) 11, 447
NciI CCSGG 1 cut(s) 383
NcoI CCATGG 1 cut(s) 265
NdeI CATATG 1 cut(s) 94
NdeII GATC 6 cut(s) 127, 217, 535, 619, 736, 925
NheI GCTAGC 2 cut(s) 410, 553
NlaIII CATG 6 cut(s) 119, 269, 535, 596, 626, 913
NlaIV GGNNCC 1 cut(s) 504
NmeAIII GCCGAG 1 cut(s) 476
NmuCI GTSAC 2 cut(s) 37, 631
NsiI ATGCAT 1 cut(s) 783
NspI RCATGY 1 cut(s) 596
PauI GCGCGC 1 cut(s) 199
PciSI GCTCTTC 1 cut(s) 189
PfeI GAWTC 1 cut(s) 748
PkrI GCNGC 3 cut(s) 7, 542, 899
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PsiI TTATAA 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 1 cut(s) 504
PspPI GGNCC 4 cut(s) 370, 503, 704, 786
PstI CTGCAG 1 cut(s) 10
PteI GCGCGC 1 cut(s) 199
RsaI GTAC 4 cut(s) 273, 345, 767, 854
RsaNI GTAC 4 cut(s) 272, 344, 766, 853
SapI GCTCTTC 1 cut(s) 189
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 3 cut(s) 6, 541, 898
Sau3AI GATC 6 cut(s) 127, 217, 535, 619, 736, 925
Sau96I GGNCC 4 cut(s) 370, 503, 704, 786
ScaI AGTACT 1 cut(s) 345
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 2 cut(s) 23, 383
SduI GDGCHC 1 cut(s) 678
SfaNI GCATC 3 cut(s) 54, 768, 790
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 1 cut(s) 370
SmlI CTYRAG 1 cut(s) 15
SmoI CTYRAG 1 cut(s) 15
Sse9I AATT 2 cut(s) 228, 258
SsiI CCGC 3 cut(s) 540, 557, 897
SspMI CTAG 5 cut(s) 56, 411, 554, 629, 940
StyD4I CCNGG 2 cut(s) 21, 381
StyI CCWWGG 2 cut(s) 265, 462
TaaI ACNGT 2 cut(s) 697, 793
TaqI TCGA 1 cut(s) 818
TasI AATT 2 cut(s) 228, 258
TatI WGTACW 2 cut(s) 343, 765
TauI GCSGC 2 cut(s) 543, 900
TfiI GAWTC 1 cut(s) 748
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 3 cut(s) 424, 700, 798
TseFI GTSAC 2 cut(s) 37, 631
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 2 cut(s) 37, 631
TspDTI ATGAA 7 cut(s) 135, 241, 302, 542, 671, 878, 926
TspRI CASTG 3 cut(s) 424, 700, 798
VpaK11BI GGWCC 1 cut(s) 370
XagI CCTNNNNNAGG 1 cut(s) 287
XapI RAATTY 1 cut(s) 228
XceI RCATGY 1 cut(s) 596
XmiI GTMKAC 2 cut(s) 604, 917
XspI CTAG 5 cut(s) 56, 411, 554, 629, 940
ZrmI AGTACT 1 cut(s) 345
Zsp2I ATGCAT 1 cut(s) 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.