Rh7AG222800

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000481
Physical Location & Seq
Reverse (-)
20828654 .. 20829989
1336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG222800.1

Sequence Viewer

Length: 903 bp
ATGTCTCTTCTCACCTCTGGTTCTCCAGATCAGCGCACCCAAATCGTCCACGATCTTGTCAAAATTTGTGAAGAATGGGGCTTCTTCATAGCGATTAACCACGGAGTACAGGAGAGCCTAATGAAGGGCATGATCGAGGCATACCATGGATTTTTCAGTCTTCCAGATGAAGAAAAGGAGGAGTTTAAGTTGGGAAATGATGTTCTCGAGATGTTCAATTATGGAACAAGCTATAATCTAGCACTGGACAAAGTACTTTTATGGAGGGACTTCTTCAAGGTCCGAACACATCCTGAGTTTCACTCCCACTACAAACCAGCTAGCTTCAGTGAGATTTCGCTAGAGTTTAGCAAAAGAAGCCGAGAAGTAGCCTTAGAAATAACAAGAGCAATATCGGAAAGCTTGGGGTTGCAGCCGGACTATATATACAACACAATGAACATGGATCGTGGCTTACAAATGCTAGCCGCTAACTACTACCCACCTTGTCCTCAGCCTCAAAATGCAATTGGTATACCCCATCATACCGATCCTGGTCTAGTCACACTCGTCATCCAAAATGAGATGAATGGTCTCCAAGTAGAGCACAATGGAAAATGGCTCACTGTGAATGGCCCTCCCAACGGCTTTTTTGTTAACCTTGCTGATCAAATGCAGATTCTTACGAACGGTAAGTACAAGAGTGTGATGCACCGAGCCACAGTGAACAACAAAGCCACAAGAATATCGATAGCTATACCACATGGACCATCCATTGACACGATCATAGCACCAGCACCAGAGTTGTGTGAAAGAGAAGGCCAAGCTCCAAAATACCTTGCAATGAACTACAAGGAATACATGCAACTTGTGCAAAGCGGCAAGAACTACATGAAGGCAACACTTGATCATATCCGAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

300

Amino Acids

34.07

Weight (kDa)

6.06

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 8 - 106 9.9e-22 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 153 - 247 1.1e-29 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 514
AciI CCGC 2 cut(s) 468, 858
AclWI GGATC 2 cut(s) 453, 524
AcsI RAATTY 1 cut(s) 63
AcuI CTGAAG 1 cut(s) 310
AfaI GTAC 3 cut(s) 108, 255, 677
AfiI CCNNNNNNNGG 2 cut(s) 124, 623
AgsI TTSAA 2 cut(s) 217, 277
AjnI CCWGG 1 cut(s) 532
AloI GAACNNNNNNTCC 2 cut(s) 186, 218
AluBI AGCT 6 cut(s) 231, 320, 324, 402, 734, 806
AluI AGCT 6 cut(s) 231, 320, 324, 402, 734, 806
Alw21I GWGCWC 1 cut(s) 588
Alw26I GTCTC 2 cut(s) 9, 578
AlwI GGATC 2 cut(s) 453, 524
Ama87I CYCGRG 1 cut(s) 206
AoxI GGCC 2 cut(s) 613, 799
ApeKI GCWGC 1 cut(s) 412
ApoI RAATTY 1 cut(s) 63
AspLEI GCGC 1 cut(s) 36
AspS9I GGNCC 3 cut(s) 280, 614, 746
AsuHPI GGTGA 1 cut(s) 4
AsuNHI GCTAGC 2 cut(s) 320, 463
AvaI CYCGRG 1 cut(s) 206
AvaII GGWCC 2 cut(s) 280, 746
BbsI GAAGAC 1 cut(s) 152
Bbv12I GWGCWC 1 cut(s) 588
BbvCI CCTCAGC 1 cut(s) 492
BbvI GCAGC 1 cut(s) 424
BccI CCATC 2 cut(s) 528, 757
BceAI ACGGC 1 cut(s) 640
BcgI CGANNNNNNTGC 2 cut(s) 25, 59
BciT130I CCWGG 1 cut(s) 534
BclI TGATCA 2 cut(s) 646, 886
BcoDI GTCTC 2 cut(s) 9, 578
BfaI CTAG 6 cut(s) 239, 321, 341, 464, 539, 901
BisI GCNGC 3 cut(s) 413, 468, 859
BlsI GCNGC 3 cut(s) 414, 469, 860
BmcAI AGTACT 1 cut(s) 255
Bme1390I CCNGG 1 cut(s) 534
Bme18I GGWCC 2 cut(s) 280, 746
BmeT110I CYCGRG 1 cut(s) 206
BmgT120I GGNCC 3 cut(s) 280, 614, 746
BmrFI CCNGG 1 cut(s) 534
BmsI GCATC 1 cut(s) 678
BmtI GCTAGC 2 cut(s) 324, 467
BpiI GAAGAC 1 cut(s) 152
BplI GAGNNNNNCTC 2 cut(s) 287, 319
BpmI CTGGAG 1 cut(s) 9
Bpu10I CCTNAGC 1 cut(s) 492
Bsa29I ATCGAT 1 cut(s) 728
BsaI GGTCTC 1 cut(s) 578
BsaJI CCNNGG 2 cut(s) 100, 145
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 623
Bse1I ACTGG 1 cut(s) 249
Bse3DI GCAATG 1 cut(s) 828
BseBI CCWGG 1 cut(s) 534
BseCI ATCGAT 1 cut(s) 728
BseDI CCNNGG 2 cut(s) 100, 145
BseGI GGATG 3 cut(s) 289, 552, 749
BseLI CCNNNNNNNGG 2 cut(s) 124, 623
BseMI GCAATG 1 cut(s) 828
BseMII CTCAG 2 cut(s) 285, 506
BseNI ACTGG 1 cut(s) 249
BseRI GAGGAG 1 cut(s) 194
BseXI GCAGC 1 cut(s) 424
BshFI GGCC 2 cut(s) 615, 801
BshVI ATCGAT 1 cut(s) 728
BsiHKAI GWGCWC 1 cut(s) 588
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 1 cut(s) 416
BslFI GGGAC 1 cut(s) 281
BslI CCNNNNNNNGG 2 cut(s) 124, 623
BsmAI GTCTC 2 cut(s) 9, 578
BsmFI GGGAC 1 cut(s) 281
BsnI GGCC 2 cut(s) 615, 801
Bso31I GGTCTC 1 cut(s) 578
BsoBI CYCGRG 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 588
Bsp143I GATC 8 cut(s) 28, 52, 132, 445, 529, 646, 762, 886
Bsp19I CCATGG 1 cut(s) 145
BspACI CCGC 2 cut(s) 468, 858
BspANI GGCC 2 cut(s) 615, 801
BspCNI CTCAG 2 cut(s) 286, 505
BspDI ATCGAT 1 cut(s) 728
BspOI GCTAGC 2 cut(s) 324, 467
BspPI GGATC 2 cut(s) 453, 524
BspTNI GGTCTC 1 cut(s) 578
BsrDI GCAATG 1 cut(s) 828
BsrI ACTGG 1 cut(s) 249
BssECI CCNNGG 2 cut(s) 100, 145
BssMI GATC 8 cut(s) 28, 52, 132, 445, 529, 646, 762, 886
BssNAI GTATAC 1 cut(s) 515
BssT1I CCWWGG 1 cut(s) 145
Bst1107I GTATAC 1 cut(s) 515
Bst2UI CCWGG 1 cut(s) 534
Bst4CI ACNGT 3 cut(s) 607, 671, 703
Bst6I CTCTTC 1 cut(s) 12
BstAPI GCANNNNNTGC 1 cut(s) 850
BstC8I GCNNGC 2 cut(s) 322, 465
BstDEI CTNAG 3 cut(s) 294, 373, 492
BstDSI CCRYGG 2 cut(s) 100, 145
BstENI CCTNNNNNAGG 1 cut(s) 122
BstF5I GGATG 3 cut(s) 289, 552, 749
BstHHI GCGC 1 cut(s) 36
BstKTI GATC 8 cut(s) 31, 55, 135, 448, 532, 649, 765, 889
BstMAI GTCTC 2 cut(s) 9, 578
BstMBI GATC 8 cut(s) 28, 52, 132, 445, 529, 646, 762, 886
BstMWI GCNNNNNNNGC 2 cut(s) 357, 850
BstNI CCWGG 1 cut(s) 534
BstNSI RCATGY 1 cut(s) 844
BstSCI CCNGG 1 cut(s) 532
BstV1I GCAGC 1 cut(s) 424
BstV2I GAAGAC 1 cut(s) 152
BstZ17I GTATAC 1 cut(s) 515
Bsu15I ATCGAT 1 cut(s) 728
BsuRI GGCC 2 cut(s) 615, 801
BsuTUI ATCGAT 1 cut(s) 728
BtgI CCRYGG 2 cut(s) 100, 145
BtsCI GGATG 3 cut(s) 289, 552, 749
BtsIMutI CAGTG 4 cut(s) 242, 334, 603, 708
Cac8I GCNNGC 2 cut(s) 322, 465
CfoI GCGC 1 cut(s) 36
Cfr13I GGNCC 3 cut(s) 280, 614, 746
ClaI ATCGAT 1 cut(s) 728
Csp6I GTAC 3 cut(s) 107, 254, 676
CviAII CATG 6 cut(s) 130, 146, 442, 743, 841, 871
CviQI GTAC 3 cut(s) 107, 254, 676
DdeI CTNAG 3 cut(s) 294, 373, 492
DpnI GATC 8 cut(s) 30, 54, 134, 447, 531, 648, 764, 888
DpnII GATC 8 cut(s) 28, 52, 132, 445, 529, 646, 762, 886
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 145
Eco31I GGTCTC 1 cut(s) 578
Eco47I GGWCC 2 cut(s) 280, 746
Eco57I CTGAAG 1 cut(s) 310
Eco88I CYCGRG 1 cut(s) 206
EcoNI CCTNNNNNAGG 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 532
EcoT14I CCWWGG 1 cut(s) 145
ErhI CCWWGG 1 cut(s) 145
FaeI CATG 6 cut(s) 133, 149, 445, 746, 844, 874
FaqI GGGAC 1 cut(s) 281
FatI CATG 6 cut(s) 129, 145, 441, 742, 840, 870
FbaI TGATCA 2 cut(s) 646, 886
FblI GTMKAC 1 cut(s) 514
Fnu4HI GCNGC 3 cut(s) 413, 468, 859
FokI GGATG 3 cut(s) 276, 539, 736
Fsp4HI GCNGC 3 cut(s) 413, 468, 859
FspBI CTAG 6 cut(s) 239, 321, 341, 464, 539, 901
GlaI GCGC 1 cut(s) 35
GluI GCNGC 3 cut(s) 413, 468, 859
GsuI CTGGAG 1 cut(s) 9
HaeIII GGCC 2 cut(s) 615, 801
HapII CCGG 1 cut(s) 416
HhaI GCGC 1 cut(s) 36
Hin1II CATG 6 cut(s) 133, 149, 445, 746, 844, 874
Hin6I GCGC 1 cut(s) 34
HinP1I GCGC 1 cut(s) 34
HincII GTYRAC 1 cut(s) 637
HindII GTYRAC 1 cut(s) 637
HindIII AAGCTT 1 cut(s) 400
HinfI GANTC 1 cut(s) 658
HpaI GTTAAC 1 cut(s) 637
HpaII CCGG 1 cut(s) 416
HphI GGTGA 1 cut(s) 4
Hpy166II GTNNAC 4 cut(s) 49, 515, 637, 706
Hpy188I TCNGA 3 cut(s) 284, 397, 896
Hpy188III TCNNGA 5 cut(s) 26, 164, 206, 208, 293
Hpy8I GTNNAC 4 cut(s) 49, 515, 637, 706
HpyAV CCTTC 3 cut(s) 118, 791, 868
HpyCH4III ACNGT 3 cut(s) 607, 671, 703
HpyCH4V TGCA 7 cut(s) 412, 506, 655, 691, 821, 844, 853
HpyF10VI GCNNNNNNNGC 2 cut(s) 357, 850
HpyF3I CTNAG 3 cut(s) 294, 373, 492
Hsp92II CATG 6 cut(s) 133, 149, 445, 746, 844, 874
HspAI GCGC 1 cut(s) 34
Ksp22I TGATCA 2 cut(s) 646, 886
KspAI GTTAAC 1 cut(s) 637
Kzo9I GATC 8 cut(s) 28, 52, 132, 445, 529, 646, 762, 886
LmnI GCTCC 1 cut(s) 811
Lsp1109I GCAGC 1 cut(s) 424
LweI GCATC 1 cut(s) 678
MaeI CTAG 6 cut(s) 239, 321, 341, 464, 539, 901
MaeIII GTNAC 1 cut(s) 541
MalI GATC 8 cut(s) 30, 54, 134, 447, 531, 648, 764, 888
MboI GATC 8 cut(s) 28, 52, 132, 445, 529, 646, 762, 886
MboII GAAGA 5 cut(s) 76, 83, 152, 182, 265
MfeI CAATTG 1 cut(s) 507
MhlI GDGCHC 1 cut(s) 588
MluCI AATT 3 cut(s) 63, 217, 507
MnlI CCTC 7 cut(s) 25, 130, 172, 258, 501, 507, 627
MseI TTAA 3 cut(s) 96, 186, 636
MspI CCGG 1 cut(s) 416
MspR9I CCNGG 1 cut(s) 534
MunI CAATTG 1 cut(s) 507
MvaI CCWGG 1 cut(s) 534
MwoI GCNNNNNNNGC 2 cut(s) 357, 850
NcoI CCATGG 1 cut(s) 145
NdeII GATC 8 cut(s) 28, 52, 132, 445, 529, 646, 762, 886
NheI GCTAGC 2 cut(s) 320, 463
NlaIII CATG 6 cut(s) 133, 149, 445, 746, 844, 874
NmeAIII GCCGAG 1 cut(s) 386
NmuCI GTSAC 1 cut(s) 541
NspI RCATGY 1 cut(s) 844
PaeR7I CTCGAG 1 cut(s) 206
PfeI GAWTC 1 cut(s) 658
PkrI GCNGC 3 cut(s) 414, 469, 860
Psp6I CCWGG 1 cut(s) 532
PspGI CCWGG 1 cut(s) 532
PspPI GGNCC 3 cut(s) 280, 614, 746
PsrI GAACNNNNNNTAC 2 cut(s) 659, 691
RsaI GTAC 3 cut(s) 108, 255, 677
RsaNI GTAC 3 cut(s) 107, 254, 676
SaqAI TTAA 3 cut(s) 96, 186, 636
SatI GCNGC 3 cut(s) 413, 468, 859
Sau3AI GATC 8 cut(s) 28, 52, 132, 445, 529, 646, 762, 886
Sau96I GGNCC 3 cut(s) 280, 614, 746
ScaI AGTACT 1 cut(s) 255
ScrFI CCNGG 1 cut(s) 534
SduI GDGCHC 1 cut(s) 588
SfaNI GCATC 1 cut(s) 678
Sfr274I CTCGAG 1 cut(s) 206
SinI GGWCC 2 cut(s) 280, 746
SlaI CTCGAG 1 cut(s) 206
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 3 cut(s) 63, 217, 507
SsiI CCGC 2 cut(s) 468, 858
SspMI CTAG 6 cut(s) 239, 321, 341, 464, 539, 901
StyD4I CCNGG 1 cut(s) 532
StyI CCWWGG 1 cut(s) 145
TaaI ACNGT 3 cut(s) 607, 671, 703
TaqI TCGA 3 cut(s) 135, 207, 728
TasI AATT 3 cut(s) 63, 217, 507
TatI WGTACW 3 cut(s) 106, 253, 675
TauI GCSGC 2 cut(s) 470, 861
TfiI GAWTC 1 cut(s) 658
Tru1I TTAA 3 cut(s) 96, 186, 636
Tru9I TTAA 3 cut(s) 96, 186, 636
TscAI CASTG 4 cut(s) 249, 334, 610, 708
TseFI GTSAC 1 cut(s) 541
TseI GCWGC 1 cut(s) 412
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 7 cut(s) 76, 137, 183, 452, 581, 839, 887
TspGWI ACGGA 1 cut(s) 117
TspRI CASTG 4 cut(s) 249, 334, 610, 708
VpaK11BI GGWCC 2 cut(s) 280, 746
XagI CCTNNNNNAGG 1 cut(s) 122
XapI RAATTY 1 cut(s) 63
XceI RCATGY 1 cut(s) 844
XhoI CTCGAG 1 cut(s) 206
XmiI GTMKAC 1 cut(s) 514
XspI CTAG 6 cut(s) 239, 321, 341, 464, 539, 901
ZrmI AGTACT 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.