MD03G1140300.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000481
Physical Location & Seq
Forward (+)
14633796 .. 14635511
1716 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1140300.v1.1.491

Sequence Viewer

Length: 1062 bp
ATGGCTTCAGTTGCTACTCAAACCATGGATGCAAAGAGCATCAAATCGCTTGCGGAATCACCCGCTCTCGGCTCTGTTCCTTCTGCTTATGCCTTCAACATAAACCCCAACGATGAAGCAGATCCAAACGATCCCGAATTTGCAATCCCCATAGTTGATATGTCTCTTCTCACATCGGGATCTCCTGATCAACGGTCCAAAATAATCCACAACCTCGTCAAAATTTGCCAAGAATGGGGCTTTTTCATTGCAATTAACCATGGAGTCCCAGAGAGTCTAATGAAGGGGATGATTGACGCGTGCCATGGATTTTTCAGCCTCCCAGACGAAGAGAAGGAGGAGTTCAAATCCGGAAATGATGTGCTGGAGATGTTCAAGTACGGAACCAGCTACAATCTTGCATTAGACAAATTTCTTCTCTGGAGGGACTTCTTCAAGGTGAGAGTACATCCCGAGTTCTACTCCCTCTACAAACCGGCTTGCTTCAGTGAGGTTTCAATGGAGTTCAGCAAGAGAACAAGAGAAGTAGCATTGGAGATAACACGCGCAATCTCCGAAAGTTTGGGACTGGAGCCCAATTACATCCATGATGCGATGAACATGGATCGCGGCCTACAAATGCTCGCCGCCAACTACTATCCGCCTTGCCCTCAGCCAGAACATGCAATTGGTATACCACATCACACTGATCATGGCCTTGTCACCCTCCTCATCCAGAATGAGATGAATGGCCTCCAAGTTGAACATAATGGCAAGTGGCTCACCGTCAATGGCCCTGCCAACGGTTTCTTTGTCAACCTCGCTGACCAAATGCAAATTCTTACTAATGGCAAATACAAAAGTGTGATGCATCGGGCAACTGTGAACAGCAAAGCGACGAGGATATCTATTGCCATACCACATGGACCATCTGTTGATACACTTATCGCACCGGCACCGGAGTTACTGGAAAAAGAAGGCCAAGCTCCGAAATACATTGGAATGAATTATAAGGAGTATATCCAGCTTCAACAGAGCGGCAAGAACTACATGAAATCCACCTTTGATCACATACTAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

354

Amino Acids

39.47

Weight (kDa)

5.62

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 49 - 152 3.4e-24 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 206 - 300 8.1e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 990
AccB1I GGYRCC 1 cut(s) 934
AccBSI CCGCTC 2 cut(s) 65, 1017
AccI GTMKAC 1 cut(s) 673
AccII CGCG 3 cut(s) 299, 546, 609
AccIII TCCGGA 1 cut(s) 350
AciI CCGC 6 cut(s) 53, 63, 609, 627, 641, 1017
AclWI GGATC 4 cut(s) 116, 125, 187, 612
AcsI RAATTY 4 cut(s) 137, 222, 410, 816
AcuI CTGAAG 1 cut(s) 469
AfaI GTAC 2 cut(s) 380, 447
AfiI CCNNNNNNNGG 3 cut(s) 68, 235, 782
AflIII ACRYGT 1 cut(s) 297
AgsI TTSAA 7 cut(s) 97, 346, 376, 436, 498, 743, 1010
AjuI GAANNNNNNNTTGG 4 cut(s) 651, 683, 801, 833
AluBI AGCT 3 cut(s) 390, 965, 1006
AluI AGCT 3 cut(s) 390, 965, 1006
Alw26I GTCTC 1 cut(s) 168
AlwI GGATC 4 cut(s) 116, 125, 187, 612
Ama87I CYCGRG 1 cut(s) 452
Aor13HI TCCGGA 1 cut(s) 350
AoxI GGCC 5 cut(s) 610, 694, 730, 772, 958
ApoI RAATTY 4 cut(s) 137, 222, 410, 816
AspLEI GCGC 1 cut(s) 548
AspS9I GGNCC 3 cut(s) 195, 773, 905
AsuHPI GGTGA 4 cut(s) 51, 451, 694, 754
AvaI CYCGRG 1 cut(s) 452
AvaII GGWCC 2 cut(s) 195, 905
BanI GGYRCC 1 cut(s) 934
BanII GRGCYC 1 cut(s) 576
BbvCI CCTCAGC 1 cut(s) 651
BccI CCATC 1 cut(s) 916
BclI TGATCA 3 cut(s) 187, 688, 1045
BcoDI GTCTC 1 cut(s) 168
BfaI CTAG 1 cut(s) 1055
BisI GCNGC 3 cut(s) 610, 627, 1018
BlsI GCNGC 3 cut(s) 611, 628, 1019
Bme18I GGWCC 2 cut(s) 195, 905
BmeT110I CYCGRG 1 cut(s) 452
BmgT120I GGNCC 3 cut(s) 195, 773, 905
BmiI GGNNCC 3 cut(s) 385, 573, 936
BmsI GCATC 5 cut(s) 19, 48, 580, 837, 859
BplI GAGNNNNNCTC 2 cut(s) 446, 478
BpmI CTGGAG 3 cut(s) 386, 442, 590
Bpu10I CCTNAGC 1 cut(s) 651
BsaJI CCNNGG 3 cut(s) 24, 259, 304
BsaWI WCCGGW 2 cut(s) 350, 937
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 235, 782
Bse118I RCCGGY 2 cut(s) 475, 931
Bse1I ACTGG 2 cut(s) 573, 951
Bse3DI GCAATG 1 cut(s) 246
BseAI TCCGGA 1 cut(s) 350
BseDI CCNNGG 3 cut(s) 24, 259, 304
BseGI GGATG 5 cut(s) 34, 294, 448, 582, 711
BseLI CCNNNNNNNGG 3 cut(s) 68, 235, 782
BseMI GCAATG 1 cut(s) 246
BseMII CTCAG 1 cut(s) 665
BseNI ACTGG 2 cut(s) 573, 951
BseRI GAGGAG 2 cut(s) 353, 698
Bsh1236I CGCG 3 cut(s) 299, 546, 609
BshFI GGCC 5 cut(s) 612, 696, 732, 774, 960
BshNI GGYRCC 1 cut(s) 934
BsiHKCI CYCGRG 1 cut(s) 452
BsiSI CCGG 4 cut(s) 351, 476, 932, 938
BslFI GGGAC 3 cut(s) 251, 440, 579
BslI CCNNNNNNNGG 3 cut(s) 68, 235, 782
BsmAI GTCTC 1 cut(s) 168
BsmFI GGGAC 3 cut(s) 251, 440, 579
BsnI GGCC 5 cut(s) 612, 696, 732, 774, 960
BsoBI CYCGRG 1 cut(s) 452
Bsp1286I GDGCHC 1 cut(s) 576
Bsp13I TCCGGA 1 cut(s) 350
Bsp143I GATC 7 cut(s) 121, 130, 179, 187, 604, 688, 1045
Bsp19I CCATGG 3 cut(s) 24, 259, 304
BspACI CCGC 6 cut(s) 53, 63, 609, 627, 641, 1017
BspANI GGCC 5 cut(s) 612, 696, 732, 774, 960
BspCNI CTCAG 1 cut(s) 664
BspEI TCCGGA 1 cut(s) 350
BspFNI CGCG 3 cut(s) 299, 546, 609
BspLI GGNNCC 3 cut(s) 385, 573, 936
BspPI GGATC 4 cut(s) 116, 125, 187, 612
BspT107I GGYRCC 1 cut(s) 934
BsrBI CCGCTC 2 cut(s) 65, 1017
BsrDI GCAATG 1 cut(s) 246
BsrFI RCCGGY 2 cut(s) 475, 931
BsrI ACTGG 2 cut(s) 573, 951
BssAI RCCGGY 2 cut(s) 475, 931
BssECI CCNNGG 3 cut(s) 24, 259, 304
BssMI GATC 7 cut(s) 121, 130, 179, 187, 604, 688, 1045
BssNAI GTATAC 1 cut(s) 674
BssT1I CCWWGG 3 cut(s) 24, 259, 304
Bst1107I GTATAC 1 cut(s) 674
Bst4CI ACNGT 4 cut(s) 195, 766, 785, 862
Bst6I CTCTTC 2 cut(s) 171, 324
BstC8I GCNNGC 4 cut(s) 51, 301, 481, 624
BstDEI CTNAG 1 cut(s) 651
BstDSI CCRYGG 3 cut(s) 24, 259, 304
BstF5I GGATG 5 cut(s) 34, 294, 448, 582, 711
BstFNI CGCG 3 cut(s) 299, 546, 609
BstHHI GCGC 1 cut(s) 548
BstKTI GATC 7 cut(s) 124, 133, 182, 190, 607, 691, 1048
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 7 cut(s) 121, 130, 179, 187, 604, 688, 1045
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 665
BstUI CGCG 3 cut(s) 299, 546, 609
BstX2I RGATCY 2 cut(s) 121, 179
BstYI RGATCY 2 cut(s) 121, 179
BstZ17I GTATAC 1 cut(s) 674
BsuRI GGCC 5 cut(s) 612, 696, 732, 774, 960
BtgI CCRYGG 3 cut(s) 24, 259, 304
BtgZI GCGATG 1 cut(s) 608
BtsCI GGATG 5 cut(s) 34, 294, 448, 582, 711
BtsIMutI CAGTG 2 cut(s) 493, 684
Cac8I GCNNGC 4 cut(s) 51, 301, 481, 624
CfoI GCGC 1 cut(s) 548
Cfr10I RCCGGY 2 cut(s) 475, 931
Cfr13I GGNCC 3 cut(s) 195, 773, 905
CseI GACGC 1 cut(s) 305
Csp6I GTAC 2 cut(s) 379, 446
CviAII CATG 9 cut(s) 25, 260, 305, 587, 601, 662, 692, 902, 1030
CviQI GTAC 2 cut(s) 379, 446
DdeI CTNAG 1 cut(s) 651
DpnI GATC 7 cut(s) 123, 132, 181, 189, 606, 690, 1047
DpnII GATC 7 cut(s) 121, 130, 179, 187, 604, 688, 1045
Eam1104I CTCTTC 2 cut(s) 171, 324
EarI CTCTTC 2 cut(s) 171, 324
EciI GGCGGA 1 cut(s) 630
Eco130I CCWWGG 3 cut(s) 24, 259, 304
Eco24I GRGCYC 1 cut(s) 576
Eco32I GATATC 1 cut(s) 885
Eco47I GGWCC 2 cut(s) 195, 905
Eco57I CTGAAG 1 cut(s) 469
Eco88I CYCGRG 1 cut(s) 452
EcoRV GATATC 1 cut(s) 885
EcoT14I CCWWGG 3 cut(s) 24, 259, 304
EcoT22I ATGCAT 1 cut(s) 852
EcoT38I GRGCYC 1 cut(s) 576
ErhI CCWWGG 3 cut(s) 24, 259, 304
FaeI CATG 9 cut(s) 28, 263, 308, 590, 604, 665, 695, 905, 1033
FaqI GGGAC 3 cut(s) 251, 440, 579
FatI CATG 9 cut(s) 24, 259, 304, 586, 600, 661, 691, 901, 1029
FauI CCCGC 1 cut(s) 70
FbaI TGATCA 3 cut(s) 187, 688, 1045
FblI GTMKAC 1 cut(s) 673
Fnu4HI GCNGC 3 cut(s) 610, 627, 1018
FokI GGATG 5 cut(s) 41, 301, 435, 569, 698
FriOI GRGCYC 1 cut(s) 576
Fsp4HI GCNGC 3 cut(s) 610, 627, 1018
FspBI CTAG 1 cut(s) 1055
GlaI GCGC 1 cut(s) 547
GluI GCNGC 3 cut(s) 610, 627, 1018
GsuI CTGGAG 3 cut(s) 386, 442, 590
HaeIII GGCC 5 cut(s) 612, 696, 732, 774, 960
HapII CCGG 4 cut(s) 351, 476, 932, 938
HgaI GACGC 1 cut(s) 305
HhaI GCGC 1 cut(s) 548
Hin1II CATG 9 cut(s) 28, 263, 308, 590, 604, 665, 695, 905, 1033
Hin6I GCGC 1 cut(s) 546
HinP1I GCGC 1 cut(s) 546
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HinfI GANTC 3 cut(s) 56, 264, 274
HpaII CCGG 4 cut(s) 351, 476, 932, 938
HphI GGTGA 4 cut(s) 51, 451, 694, 754
Hpy166II GTNNAC 3 cut(s) 674, 796, 865
Hpy188I TCNGA 2 cut(s) 556, 969
Hpy188III TCNNGA 7 cut(s) 134, 177, 185, 351, 421, 452, 715
Hpy8I GTNNAC 3 cut(s) 674, 796, 865
Hpy99I CGWCG 1 cut(s) 880
HpyAV CCTTC 5 cut(s) 90, 103, 277, 328, 950
HpyCH4III ACNGT 4 cut(s) 195, 766, 785, 862
HpyCH4V TGCA 7 cut(s) 32, 143, 251, 401, 665, 814, 850
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 651
Hsp92II CATG 9 cut(s) 28, 263, 308, 590, 604, 665, 695, 905, 1033
HspAI GCGC 1 cut(s) 546
Kpn2I TCCGGA 1 cut(s) 350
Ksp22I TGATCA 3 cut(s) 187, 688, 1045
Kzo9I GATC 7 cut(s) 121, 130, 179, 187, 604, 688, 1045
LmnI GCTCC 2 cut(s) 571, 970
LweI GCATC 5 cut(s) 19, 48, 580, 837, 859
MaeI CTAG 1 cut(s) 1055
MaeIII GTNAC 2 cut(s) 700, 942
MalI GATC 7 cut(s) 123, 132, 181, 189, 606, 690, 1047
MbiI CCGCTC 2 cut(s) 65, 1017
MboI GATC 7 cut(s) 121, 130, 179, 187, 604, 688, 1045
MboII GAAGA 4 cut(s) 158, 341, 407, 424
MfeI CAATTG 1 cut(s) 666
MflI RGATCY 2 cut(s) 121, 179
MhlI GDGCHC 1 cut(s) 576
MluCI AATT 8 cut(s) 137, 222, 252, 410, 577, 666, 816, 985
MluI ACGCGT 1 cut(s) 297
MlyI GAGTC 2 cut(s) 273, 283
Mph1103I ATGCAT 1 cut(s) 852
MroI TCCGGA 1 cut(s) 350
MseI TTAA 1 cut(s) 255
MslI CAYNNNNRTG 1 cut(s) 980
MspI CCGG 4 cut(s) 351, 476, 932, 938
MunI CAATTG 1 cut(s) 666
MvnI CGCG 3 cut(s) 299, 546, 609
MwoI GCNNNNNNNGC 1 cut(s) 11
NcoI CCATGG 3 cut(s) 24, 259, 304
NdeII GATC 7 cut(s) 121, 130, 179, 187, 604, 688, 1045
NlaIII CATG 9 cut(s) 28, 263, 308, 590, 604, 665, 695, 905, 1033
NlaIV GGNNCC 3 cut(s) 385, 573, 936
NmeAIII GCCGAG 1 cut(s) 48
NmuCI GTSAC 1 cut(s) 700
NsiI ATGCAT 1 cut(s) 852
NspI RCATGY 1 cut(s) 665
PfeI GAWTC 1 cut(s) 56
PkrI GCNGC 3 cut(s) 611, 628, 1019
PleI GAGTC 2 cut(s) 272, 282
PpsI GAGTC 2 cut(s) 272, 282
PsiI TTATAA 1 cut(s) 990
PspN4I GGNNCC 3 cut(s) 385, 573, 936
PspPI GGNCC 3 cut(s) 195, 773, 905
PsuI RGATCY 2 cut(s) 121, 179
RsaI GTAC 2 cut(s) 380, 447
RsaNI GTAC 2 cut(s) 379, 446
RseI CAYNNNNRTG 1 cut(s) 980
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 3 cut(s) 610, 627, 1018
Sau3AI GATC 7 cut(s) 121, 130, 179, 187, 604, 688, 1045
Sau96I GGNCC 3 cut(s) 195, 773, 905
SchI GAGTC 2 cut(s) 273, 283
SduI GDGCHC 1 cut(s) 576
SetI ASST 8 cut(s) 216, 392, 441, 495, 801, 967, 1008, 1043
SfaNI GCATC 5 cut(s) 19, 48, 580, 837, 859
SinI GGWCC 2 cut(s) 195, 905
SmiMI CAYNNNNRTG 1 cut(s) 980
Sse9I AATT 8 cut(s) 137, 222, 252, 410, 577, 666, 816, 985
SsiI CCGC 6 cut(s) 53, 63, 609, 627, 641, 1017
SspMI CTAG 1 cut(s) 1055
StyI CCWWGG 3 cut(s) 24, 259, 304
TaaI ACNGT 4 cut(s) 195, 766, 785, 862
TasI AATT 8 cut(s) 137, 222, 252, 410, 577, 666, 816, 985
TatI WGTACW 1 cut(s) 445
TauI GCSGC 3 cut(s) 612, 629, 1020
TfiI GAWTC 1 cut(s) 56
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 2 cut(s) 493, 691
TseFI GTSAC 1 cut(s) 700
Tsp45I GTSAC 1 cut(s) 700
TspDTI ATGAA 7 cut(s) 129, 235, 296, 611, 740, 998, 1046
TspGWI ACGGA 1 cut(s) 396
TspRI CASTG 2 cut(s) 493, 691
VpaK11BI GGWCC 2 cut(s) 195, 905
XapI RAATTY 4 cut(s) 137, 222, 410, 816
XceI RCATGY 1 cut(s) 665
XmiI GTMKAC 1 cut(s) 673
XspI CTAG 1 cut(s) 1055
Zsp2I ATGCAT 1 cut(s) 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.