Rroxscaffold_3G00252030

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000481
Physical Location & Seq
Forward (+)
46009858 .. 46011335
1478 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00252030.1

Sequence Viewer

Length: 1068 bp
ATGGCTGCAGTTGCTCAAGCCTTGGACACCTCAAATGTCACAAGCATAAAATCAGTAGCCGAGTCACCAGCTCTCAGCTCTGTCCCCTCTGCATATGCCTTCAACATAAACCCTAATGATGAAGCTGATCCAAATGACCCTGAGTTTGCTGTTCCCATTGTTGATATGTCTCTTCTCACCTCTGGTTCTCCTGATCAGCGCGCTCAAATCATCTGCGATCTTGTTAAAATTTGTCAAGAATGGGGCTTCTTCATTGCAATTAACCACGGAGTACCAGAGAGCCTGATGAAGGGGATGATTGACGCATGTCATGGATTTTTCAGTCTACCAGATGAAGAAAAGAAGGAGTTTAAGTCAGGAAATGATGTTCTTGAGATGTTCAAGTATGGGACCAGCTATAATCTTGCATTGGACAAAGTACTTCTGTGGAGGGACTTCTTCAAGGTCCGAACACATCCTGAGTTTCACTCCCTCTACAAACCAGCTAGCTTCAGTGAGGTTTCGCTGGAGTTTAGCAAAAGAAGCCGAGAAGTAGCCTTGGAAATAACAAGAGCAATATCGGAAAGCTTGGGTTTGGGGCCGGACTACATATACAACACAATGAACATGGATCGCGGCTTACAAATGCTAGCGGGGAACTACTACCCACCTTGTCCTCAGCCTGAACATGCCATTGGTATACCTCATCATACCGATCATGGTCTAATCACACTCCTCATCCAGAATGAGATGAATGGTCTCCAAGTTGAGCACAATGGAAAATGGGTGACAGTCAATGGCTCTCCCAATGGCTTTTTCGTGAACCTTGCTGACCAAATGCAGATTCTTACGAACGGTAAGTACAAGAGTGTGATGCATCGCGCCACCGTGAACAACAAAGCTACAAGGATATCGATAGCAATACCAAATGGACCATCCGTAGACACGATCATAGCACCAGCACCAGAGTTGTGTGAAAGAGAAGGCCAAGCTCCAAAATACCTCGCAATGAACTACAAGGAGTACATACAACTTCAGCAAAGCGGCAAGAACTACATGAAGTCAACACTTGATCATATCCGAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

355

Amino Acids

39.48

Weight (kDa)

5.57

Isoelectric Point (pI)

34.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 51 - 161 4e-25 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 208 - 301 1.1e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 325, 679, 921
AccII CGCG 3 cut(s) 201, 615, 861
AciI CCGC 3 cut(s) 615, 632, 1023
AclWI GGATC 2 cut(s) 122, 618
AcsI RAATTY 1 cut(s) 228
AcuI CTGAAG 2 cut(s) 475, 998
AfaI GTAC 4 cut(s) 273, 420, 842, 1004
AfiI CCNNNNNNNGG 1 cut(s) 289
AgsI TTSAA 3 cut(s) 103, 382, 442
AjuI GAANNNNNNNTTGG 6 cut(s) 657, 689, 779, 807, 811, 839
AloI GAACNNNNNNTCC 2 cut(s) 351, 383
AluBI AGCT 9 cut(s) 71, 78, 125, 396, 485, 489, 567, 881, 971
AluI AGCT 9 cut(s) 71, 78, 125, 396, 485, 489, 567, 881, 971
Alw21I GWGCWC 1 cut(s) 753
Alw26I GTCTC 2 cut(s) 174, 743
AlwI GGATC 2 cut(s) 122, 618
AoxI GGCC 2 cut(s) 578, 964
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 228
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AspLEI GCGC 3 cut(s) 201, 203, 863
AspS9I GGNCC 4 cut(s) 390, 445, 578, 911
AsuHPI GGTGA 3 cut(s) 57, 169, 778
AsuNHI GCTAGC 2 cut(s) 485, 628
AvaII GGWCC 3 cut(s) 390, 445, 911
Bbv12I GWGCWC 1 cut(s) 753
BbvCI CCTCAGC 1 cut(s) 657
BccI CCATC 1 cut(s) 922
BclI TGATCA 2 cut(s) 193, 1051
BcoDI GTCTC 2 cut(s) 174, 743
BfaI CTAG 3 cut(s) 486, 629, 1066
BfmI CTRYAG 1 cut(s) 6
BisI GCNGC 3 cut(s) 6, 616, 1024
BlsI GCNGC 3 cut(s) 7, 617, 1025
BmcAI AGTACT 1 cut(s) 420
Bme18I GGWCC 3 cut(s) 390, 445, 911
BmgT120I GGNCC 4 cut(s) 390, 445, 578, 911
BmiI GGNNCC 2 cut(s) 391, 579
BmsI GCATC 2 cut(s) 843, 865
BmtI GCTAGC 2 cut(s) 489, 632
BoxI GACNNNNGTC 1 cut(s) 306
BplI GAGNNNNNCTC 2 cut(s) 452, 484
BpmI CTGGAG 1 cut(s) 527
Bpu10I CCTNAGC 1 cut(s) 657
BpuEI CTTGAG 1 cut(s) 392
Bsa29I ATCGAT 1 cut(s) 893
BsaI GGTCTC 1 cut(s) 743
BsaJI CCNNGG 3 cut(s) 21, 265, 537
BsaXI ACNNNNNCTCC 2 cut(s) 338, 368
Bsc4I CCNNNNNNNGG 1 cut(s) 289
Bse3DI GCAATG 2 cut(s) 252, 993
BseCI ATCGAT 1 cut(s) 893
BseDI CCNNGG 3 cut(s) 21, 265, 537
BseGI GGATG 4 cut(s) 300, 454, 717, 914
BseLI CCNNNNNNNGG 1 cut(s) 289
BseMI GCAATG 2 cut(s) 252, 993
BseMII CTCAG 4 cut(s) 88, 132, 450, 671
BsePI GCGCGC 1 cut(s) 199
BseRI GAGGAG 1 cut(s) 704
Bsh1236I CGCG 3 cut(s) 201, 615, 861
BshFI GGCC 2 cut(s) 580, 966
BshVI ATCGAT 1 cut(s) 893
BsiHKAI GWGCWC 1 cut(s) 753
BsiSI CCGG 1 cut(s) 581
BslFI GGGAC 3 cut(s) 68, 403, 446
BslI CCNNNNNNNGG 1 cut(s) 289
BsmAI GTCTC 2 cut(s) 174, 743
BsmFI GGGAC 3 cut(s) 68, 403, 446
BsnI GGCC 2 cut(s) 580, 966
Bso31I GGTCTC 1 cut(s) 743
Bsp1286I GDGCHC 1 cut(s) 753
Bsp143I GATC 7 cut(s) 127, 193, 217, 610, 694, 927, 1051
BspACI CCGC 3 cut(s) 615, 632, 1023
BspANI GGCC 2 cut(s) 580, 966
BspCNI CTCAG 4 cut(s) 87, 133, 451, 670
BspDI ATCGAT 1 cut(s) 893
BspFNI CGCG 3 cut(s) 201, 615, 861
BspLI GGNNCC 2 cut(s) 391, 579
BspMAI CTGCAG 1 cut(s) 10
BspOI GCTAGC 2 cut(s) 489, 632
BspPI GGATC 2 cut(s) 122, 618
BspTNI GGTCTC 1 cut(s) 743
BsrDI GCAATG 2 cut(s) 252, 993
BssECI CCNNGG 3 cut(s) 21, 265, 537
BssHII GCGCGC 1 cut(s) 199
BssMI GATC 7 cut(s) 127, 193, 217, 610, 694, 927, 1051
BssNAI GTATAC 1 cut(s) 680
BssT1I CCWWGG 2 cut(s) 21, 537
Bst1107I GTATAC 1 cut(s) 680
Bst4CI ACNGT 3 cut(s) 772, 836, 868
Bst6I CTCTTC 1 cut(s) 177
BstC8I GCNNGC 3 cut(s) 201, 487, 630
BstDEI CTNAG 4 cut(s) 74, 141, 459, 657
BstDSI CCRYGG 1 cut(s) 265
BstENI CCTNNNNNAGG 1 cut(s) 287
BstF5I GGATG 4 cut(s) 300, 454, 717, 914
BstFNI CGCG 3 cut(s) 201, 615, 861
BstHHI GCGC 3 cut(s) 201, 203, 863
BstKTI GATC 7 cut(s) 130, 196, 220, 613, 697, 930, 1054
BstMAI GTCTC 2 cut(s) 174, 743
BstMBI GATC 7 cut(s) 127, 193, 217, 610, 694, 927, 1051
BstMWI GCNNNNNNNGC 2 cut(s) 11, 522
BstNSI RCATGY 2 cut(s) 309, 671
BstPAI GACNNNNGTC 1 cut(s) 306
BstSFI CTRYAG 1 cut(s) 6
BstUI CGCG 3 cut(s) 201, 615, 861
BstZ17I GTATAC 1 cut(s) 680
Bsu15I ATCGAT 1 cut(s) 893
BsuRI GGCC 2 cut(s) 580, 966
BsuTUI ATCGAT 1 cut(s) 893
BtgI CCRYGG 1 cut(s) 265
BtgZI GCGATG 1 cut(s) 842
BtsCI GGATG 4 cut(s) 300, 454, 717, 914
BtsIMutI CAGTG 1 cut(s) 499
Cac8I GCNNGC 3 cut(s) 201, 487, 630
CfoI GCGC 3 cut(s) 201, 203, 863
Cfr13I GGNCC 4 cut(s) 390, 445, 578, 911
ClaI ATCGAT 1 cut(s) 893
CseI GACGC 1 cut(s) 311
Csp6I GTAC 4 cut(s) 272, 419, 841, 1003
CviAII CATG 6 cut(s) 306, 311, 607, 668, 698, 1036
CviQI GTAC 4 cut(s) 272, 419, 841, 1003
DdeI CTNAG 4 cut(s) 74, 141, 459, 657
DpnI GATC 7 cut(s) 129, 195, 219, 612, 696, 929, 1053
DpnII GATC 7 cut(s) 127, 193, 217, 610, 694, 927, 1051
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Eco130I CCWWGG 2 cut(s) 21, 537
Eco31I GGTCTC 1 cut(s) 743
Eco32I GATATC 1 cut(s) 891
Eco47I GGWCC 3 cut(s) 390, 445, 911
Eco57I CTGAAG 2 cut(s) 475, 998
EcoNI CCTNNNNNAGG 1 cut(s) 287
EcoRV GATATC 1 cut(s) 891
EcoT14I CCWWGG 2 cut(s) 21, 537
EcoT22I ATGCAT 1 cut(s) 858
ErhI CCWWGG 2 cut(s) 21, 537
FaeI CATG 6 cut(s) 309, 314, 610, 671, 701, 1039
FaqI GGGAC 3 cut(s) 68, 403, 446
FatI CATG 6 cut(s) 305, 310, 606, 667, 697, 1035
FauI CCCGC 1 cut(s) 625
FauNDI CATATG 1 cut(s) 94
FbaI TGATCA 2 cut(s) 193, 1051
FblI GTMKAC 3 cut(s) 325, 679, 921
Fnu4HI GCNGC 3 cut(s) 6, 616, 1024
FokI GGATG 4 cut(s) 307, 441, 704, 901
Fsp4HI GCNGC 3 cut(s) 6, 616, 1024
FspBI CTAG 3 cut(s) 486, 629, 1066
GlaI GCGC 3 cut(s) 200, 202, 862
GluI GCNGC 3 cut(s) 6, 616, 1024
GsuI CTGGAG 1 cut(s) 527
HaeIII GGCC 2 cut(s) 580, 966
HapII CCGG 1 cut(s) 581
HgaI GACGC 1 cut(s) 311
HhaI GCGC 3 cut(s) 201, 203, 863
Hin1II CATG 6 cut(s) 309, 314, 610, 671, 701, 1039
Hin6I GCGC 3 cut(s) 199, 201, 861
HinP1I GCGC 3 cut(s) 199, 201, 861
HincII GTYRAC 1 cut(s) 1044
HindII GTYRAC 1 cut(s) 1044
HindIII AAGCTT 1 cut(s) 565
HinfI GANTC 2 cut(s) 62, 823
HpaII CCGG 1 cut(s) 581
HphI GGTGA 3 cut(s) 57, 169, 778
Hpy166II GTNNAC 6 cut(s) 326, 680, 802, 871, 922, 1044
Hpy188I TCNGA 3 cut(s) 449, 562, 1061
Hpy188III TCNNGA 7 cut(s) 191, 236, 357, 371, 458, 721, 799
Hpy8I GTNNAC 6 cut(s) 326, 680, 802, 871, 922, 1044
HpyAV CCTTC 4 cut(s) 109, 283, 337, 956
HpyCH4III ACNGT 3 cut(s) 772, 836, 868
HpyCH4V TGCA 6 cut(s) 8, 92, 257, 407, 820, 856
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 522
HpyF3I CTNAG 4 cut(s) 74, 141, 459, 657
Hsp92II CATG 6 cut(s) 309, 314, 610, 671, 701, 1039
HspAI GCGC 3 cut(s) 199, 201, 861
Ksp22I TGATCA 2 cut(s) 193, 1051
Kzo9I GATC 7 cut(s) 127, 193, 217, 610, 694, 927, 1051
LmnI GCTCC 1 cut(s) 976
LweI GCATC 2 cut(s) 843, 865
MaeI CTAG 3 cut(s) 486, 629, 1066
MaeIII GTNAC 3 cut(s) 37, 63, 766
MalI GATC 7 cut(s) 129, 195, 219, 612, 696, 929, 1053
MboI GATC 7 cut(s) 127, 193, 217, 610, 694, 927, 1051
MboII GAAGA 4 cut(s) 164, 241, 347, 430
MhlI GDGCHC 1 cut(s) 753
MluCI AATT 2 cut(s) 228, 258
MlyI GAGTC 1 cut(s) 71
Mph1103I ATGCAT 1 cut(s) 858
MseI TTAA 3 cut(s) 225, 261, 351
MspI CCGG 1 cut(s) 581
MvnI CGCG 3 cut(s) 201, 615, 861
MwoI GCNNNNNNNGC 2 cut(s) 11, 522
NdeI CATATG 1 cut(s) 94
NdeII GATC 7 cut(s) 127, 193, 217, 610, 694, 927, 1051
NheI GCTAGC 2 cut(s) 485, 628
NlaIII CATG 6 cut(s) 309, 314, 610, 671, 701, 1039
NlaIV GGNNCC 2 cut(s) 391, 579
NmeAIII GCCGAG 2 cut(s) 85, 551
NmuCI GTSAC 3 cut(s) 37, 63, 766
NsiI ATGCAT 1 cut(s) 858
NspI RCATGY 2 cut(s) 309, 671
PauI GCGCGC 1 cut(s) 199
PfeI GAWTC 1 cut(s) 823
PkrI GCNGC 3 cut(s) 7, 617, 1025
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PshAI GACNNNNGTC 1 cut(s) 306
PspN4I GGNNCC 2 cut(s) 391, 579
PspPI GGNCC 4 cut(s) 390, 445, 578, 911
PsrI GAACNNNNNNTAC 2 cut(s) 824, 856
PstI CTGCAG 1 cut(s) 10
PteI GCGCGC 1 cut(s) 199
RsaI GTAC 4 cut(s) 273, 420, 842, 1004
RsaNI GTAC 4 cut(s) 272, 419, 841, 1003
SaqAI TTAA 3 cut(s) 225, 261, 351
SatI GCNGC 3 cut(s) 6, 616, 1024
Sau3AI GATC 7 cut(s) 127, 193, 217, 610, 694, 927, 1051
Sau96I GGNCC 4 cut(s) 390, 445, 578, 911
ScaI AGTACT 1 cut(s) 420
SchI GAGTC 1 cut(s) 71
SduI GDGCHC 1 cut(s) 753
SfaNI GCATC 2 cut(s) 843, 865
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 3 cut(s) 390, 445, 911
SmlI CTYRAG 2 cut(s) 15, 371
SmoI CTYRAG 2 cut(s) 15, 371
Sse9I AATT 2 cut(s) 228, 258
SsiI CCGC 3 cut(s) 615, 632, 1023
SspMI CTAG 3 cut(s) 486, 629, 1066
StyI CCWWGG 2 cut(s) 21, 537
TaaI ACNGT 3 cut(s) 772, 836, 868
TaqI TCGA 1 cut(s) 893
TasI AATT 2 cut(s) 228, 258
TatI WGTACW 3 cut(s) 418, 840, 1002
TauI GCSGC 2 cut(s) 618, 1026
TfiI GAWTC 1 cut(s) 823
Tru1I TTAA 3 cut(s) 225, 261, 351
Tru9I TTAA 3 cut(s) 225, 261, 351
TscAI CASTG 1 cut(s) 499
TseFI GTSAC 3 cut(s) 37, 63, 766
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 3 cut(s) 37, 63, 766
TspDTI ATGAA 8 cut(s) 135, 241, 302, 348, 617, 746, 1004, 1052
TspGWI ACGGA 2 cut(s) 282, 907
TspRI CASTG 1 cut(s) 499
VpaK11BI GGWCC 3 cut(s) 390, 445, 911
XagI CCTNNNNNAGG 1 cut(s) 287
XapI RAATTY 1 cut(s) 228
XceI RCATGY 2 cut(s) 309, 671
XmiI GTMKAC 3 cut(s) 325, 679, 921
XspI CTAG 3 cut(s) 486, 629, 1066
ZrmI AGTACT 1 cut(s) 420
Zsp2I ATGCAT 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.