FvH4_5g20432

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000481
Physical Location & Seq
Reverse (-)
12099808 .. 12109605
9798 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20432.t4

Sequence Viewer

Length: 1074 bp
ATGGCTGCAGTAGTTGCTGATCAAGCCCTGGATAACTCAAATGTCTCGAGCATCAAATCACTAGCTGACTCACAAGCTCTCAACTCTGTGCCTTCTGCATATGCCTTCAACATCAACCCCAACGATGAAGCCGATCCTAATGACCCTGAGTTTGCTATTCCCATTGTCGATATGTCTCTTCTCACCTCTGGTTCTCCCGACGAGCGCGCGAAAATCATCCGCGATCTTGTCAAAATTTGTGAAGAATGGGGCTTCTTCATCGCAATTAACCATGGAGTACCAGAGAGCTTAATGAAGGGGATGATCGACGCATGTCATGGATTTTTCAGTCTTCCAGACGAGGAGAAGAAGGAATTCAAATCAGGAAATGATGTGCTTGAGATGTTCAAGTATGGAACAAGCTATAATCTAGCATTGGACAAAGTACTTTTATGGAGGGACTTCTTCAAGGTCCGAACACATCCCGAGTTCTTCTCCCTCTACAAACCGGCAAGCTTCAGTGAGACATCGCTGGAGTTTAGCAGAAGAAGTCGAGAAGTAGCCTTGGAAATAACAAGAGCAATATCGGAAAGTTTGGGGTTGGAGCCGGACTACATATACAACACAATGAACATGGATCGCGGCCTACAACTGCTAGCGGGGAACTACTACCCTCCTTGTCCTCAGCCTGAACATGCCATTGGTATACCCCATCATACCGATCATGGTTTAGTCACACTCCTCATCCAGAATGAGATGAATGGTCTCCAAGTTGAGCACAATGGAAAATGGCTCACTGTCAATGGCCCTAAGAATGGCTTCTTTGTTAACCTTGCAGACCAAATGCAGATTCTTACAAACGGTAAGTACAAGAGTGTGATGCATCGCGCCACCGTGAACAACAAAGCTACGAGGATATCAATAGCCATACCACATGGACCATCCGTAGACACGATCATAGCACCAGCGCCAGAGTTGTGTGAAAGAGAAGGCCAAGCACCAAAATACCTTGCTATGAACTACAAGGAATACATACAACTTCAGCAAAGTGGAAAGAACTACATGAAGTCCACATTTGATCACATCCGAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

358

Amino Acids

40.01

Weight (kDa)

5.49

Isoelectric Point (pI)

34.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 53 - 156 2.4e-25 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 210 - 304 2.5e-29 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 685, 927
AccII CGCG 5 cut(s) 207, 209, 222, 621, 867
AciI CCGC 3 cut(s) 220, 621, 638
AclWI GGATC 2 cut(s) 128, 624
AcsI RAATTY 2 cut(s) 234, 353
AcuI CTGAAG 2 cut(s) 481, 1004
AfaI GTAC 3 cut(s) 279, 426, 848
AfiI CCNNNNNNNGG 1 cut(s) 794
AgsI TTSAA 4 cut(s) 109, 358, 388, 448
AjnI CCWGG 1 cut(s) 27
AjuI GAANNNNNNNTTGG 4 cut(s) 663, 695, 813, 845
AluBI AGCT 6 cut(s) 65, 77, 288, 402, 495, 887
AluI AGCT 6 cut(s) 65, 77, 288, 402, 495, 887
Alw21I GWGCWC 1 cut(s) 759
Alw26I GTCTC 4 cut(s) 49, 180, 497, 749
AlwI GGATC 2 cut(s) 128, 624
Ama87I CYCGRG 2 cut(s) 46, 464
AoxI GGCC 3 cut(s) 622, 784, 970
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 2 cut(s) 234, 353
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
Asp700I GAANNNNTTC 2 cut(s) 353, 797
AspLEI GCGC 4 cut(s) 207, 209, 869, 949
AspS9I GGNCC 3 cut(s) 451, 785, 917
AsuHPI GGTGA 1 cut(s) 175
AsuNHI GCTAGC 1 cut(s) 634
AvaI CYCGRG 2 cut(s) 46, 464
AvaII GGWCC 2 cut(s) 451, 917
BbsI GAAGAC 1 cut(s) 323
Bbv12I GWGCWC 1 cut(s) 759
BbvCI CCTCAGC 1 cut(s) 663
BccI CCATC 2 cut(s) 699, 928
BciT130I CCWGG 1 cut(s) 29
BclI TGATCA 2 cut(s) 19, 1057
BcoDI GTCTC 4 cut(s) 49, 180, 497, 749
BfaI CTAG 4 cut(s) 62, 410, 635, 1072
BfmI CTRYAG 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 950
BisI GCNGC 2 cut(s) 6, 622
BlsI GCNGC 2 cut(s) 7, 623
BmcAI AGTACT 1 cut(s) 426
Bme1390I CCNGG 1 cut(s) 29
Bme18I GGWCC 2 cut(s) 451, 917
BmeT110I CYCGRG 2 cut(s) 46, 464
BmgT120I GGNCC 3 cut(s) 451, 785, 917
BmiI GGNNCC 1 cut(s) 585
BmrFI CCNGG 1 cut(s) 29
BmsI GCATC 3 cut(s) 60, 849, 871
BmtI GCTAGC 1 cut(s) 638
BoxI GACNNNNGTC 1 cut(s) 312
BpiI GAAGAC 1 cut(s) 323
BplI GAGNNNNNCTC 2 cut(s) 458, 490
BpmI CTGGAG 1 cut(s) 533
Bpu10I CCTNAGC 1 cut(s) 663
BpuEI CTTGAG 1 cut(s) 398
BsaI GGTCTC 1 cut(s) 749
BsaJI CCNNGG 3 cut(s) 27, 271, 543
Bsc4I CCNNNNNNNGG 1 cut(s) 794
Bse118I RCCGGY 1 cut(s) 487
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 3 cut(s) 27, 271, 543
BseGI GGATG 6 cut(s) 216, 306, 460, 723, 920, 1062
BseLI CCNNNNNNNGG 1 cut(s) 794
BseMII CTCAG 2 cut(s) 138, 677
BsePI GCGCGC 1 cut(s) 205
BseRI GAGGAG 2 cut(s) 356, 710
Bsh1236I CGCG 5 cut(s) 207, 209, 222, 621, 867
BshFI GGCC 3 cut(s) 624, 786, 972
BsiHKAI GWGCWC 1 cut(s) 759
BsiHKCI CYCGRG 2 cut(s) 46, 464
BsiSI CCGG 2 cut(s) 488, 587
BslFI GGGAC 1 cut(s) 452
BslI CCNNNNNNNGG 1 cut(s) 794
BsmAI GTCTC 4 cut(s) 49, 180, 497, 749
BsmFI GGGAC 1 cut(s) 452
BsnI GGCC 3 cut(s) 624, 786, 972
Bso31I GGTCTC 1 cut(s) 749
BsoBI CYCGRG 2 cut(s) 46, 464
Bsp1286I GDGCHC 1 cut(s) 759
Bsp143I GATC 8 cut(s) 19, 133, 223, 303, 616, 700, 933, 1057
Bsp19I CCATGG 1 cut(s) 271
BspACI CCGC 3 cut(s) 220, 621, 638
BspANI GGCC 3 cut(s) 624, 786, 972
BspCNI CTCAG 2 cut(s) 139, 676
BspFNI CGCG 5 cut(s) 207, 209, 222, 621, 867
BspLI GGNNCC 1 cut(s) 585
BspMAI CTGCAG 1 cut(s) 10
BspOI GCTAGC 1 cut(s) 638
BspPI GGATC 2 cut(s) 128, 624
BspTNI GGTCTC 1 cut(s) 749
BsrFI RCCGGY 1 cut(s) 487
BssAI RCCGGY 1 cut(s) 487
BssECI CCNNGG 3 cut(s) 27, 271, 543
BssHII GCGCGC 1 cut(s) 205
BssMI GATC 8 cut(s) 19, 133, 223, 303, 616, 700, 933, 1057
BssNAI GTATAC 1 cut(s) 686
BssT1I CCWWGG 2 cut(s) 271, 543
Bst1107I GTATAC 1 cut(s) 686
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 3 cut(s) 778, 842, 874
Bst6I CTCTTC 1 cut(s) 183
BstAPI GCANNNNNTGC 1 cut(s) 14
BstC8I GCNNGC 3 cut(s) 207, 493, 636
BstDEI CTNAG 3 cut(s) 147, 663, 789
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 6 cut(s) 216, 306, 460, 723, 920, 1062
BstFNI CGCG 5 cut(s) 207, 209, 222, 621, 867
BstH2I RGCGCY 1 cut(s) 950
BstHHI GCGC 4 cut(s) 207, 209, 869, 949
BstKTI GATC 8 cut(s) 22, 136, 226, 306, 619, 703, 936, 1060
BstMAI GTCTC 4 cut(s) 49, 180, 497, 749
BstMBI GATC 8 cut(s) 19, 133, 223, 303, 616, 700, 933, 1057
BstMWI GCNNNNNNNGC 2 cut(s) 14, 23
BstNI CCWGG 1 cut(s) 29
BstNSI RCATGY 2 cut(s) 315, 677
BstPAI GACNNNNGTC 1 cut(s) 312
BstSCI CCNGG 1 cut(s) 27
BstSFI CTRYAG 1 cut(s) 6
BstUI CGCG 5 cut(s) 207, 209, 222, 621, 867
BstV2I GAAGAC 1 cut(s) 323
BstZ17I GTATAC 1 cut(s) 686
BsuRI GGCC 3 cut(s) 624, 786, 972
BtgI CCRYGG 1 cut(s) 271
BtgZI GCGATG 3 cut(s) 244, 492, 848
BtsCI GGATG 6 cut(s) 216, 306, 460, 723, 920, 1062
BtsIMutI CAGTG 2 cut(s) 505, 774
Cac8I GCNNGC 3 cut(s) 207, 493, 636
CfoI GCGC 4 cut(s) 207, 209, 869, 949
Cfr10I RCCGGY 1 cut(s) 487
Cfr13I GGNCC 3 cut(s) 451, 785, 917
CseI GACGC 1 cut(s) 317
Csp6I GTAC 3 cut(s) 278, 425, 847
CviAII CATG 8 cut(s) 272, 312, 317, 613, 674, 704, 914, 1042
CviQI GTAC 3 cut(s) 278, 425, 847
DdeI CTNAG 3 cut(s) 147, 663, 789
DpnI GATC 8 cut(s) 21, 135, 225, 305, 618, 702, 935, 1059
DpnII GATC 8 cut(s) 19, 133, 223, 303, 616, 700, 933, 1057
Eam1104I CTCTTC 1 cut(s) 183
EarI CTCTTC 1 cut(s) 183
Eco130I CCWWGG 2 cut(s) 271, 543
Eco31I GGTCTC 1 cut(s) 749
Eco32I GATATC 1 cut(s) 897
Eco47I GGWCC 2 cut(s) 451, 917
Eco57I CTGAAG 2 cut(s) 481, 1004
Eco88I CYCGRG 2 cut(s) 46, 464
EcoRI GAATTC 1 cut(s) 353
EcoRII CCWGG 1 cut(s) 27
EcoRV GATATC 1 cut(s) 897
EcoT14I CCWWGG 2 cut(s) 271, 543
EcoT22I ATGCAT 1 cut(s) 864
ErhI CCWWGG 2 cut(s) 271, 543
FaeI CATG 8 cut(s) 275, 315, 320, 616, 677, 707, 917, 1045
FalI AAGNNNNNCTT 2 cut(s) 782, 814
FaqI GGGAC 1 cut(s) 452
FatI CATG 8 cut(s) 271, 311, 316, 612, 673, 703, 913, 1041
FauI CCCGC 1 cut(s) 631
FauNDI CATATG 1 cut(s) 100
FbaI TGATCA 2 cut(s) 19, 1057
FblI GTMKAC 2 cut(s) 685, 927
Fnu4HI GCNGC 2 cut(s) 6, 622
FokI GGATG 6 cut(s) 203, 313, 447, 710, 907, 1049
Fsp4HI GCNGC 2 cut(s) 6, 622
FspBI CTAG 4 cut(s) 62, 410, 635, 1072
GlaI GCGC 4 cut(s) 206, 208, 868, 948
GluI GCNGC 2 cut(s) 6, 622
GsuI CTGGAG 1 cut(s) 533
HaeII RGCGCY 1 cut(s) 950
HaeIII GGCC 3 cut(s) 624, 786, 972
HapII CCGG 2 cut(s) 488, 587
HgaI GACGC 1 cut(s) 317
HhaI GCGC 4 cut(s) 207, 209, 869, 949
Hin1II CATG 8 cut(s) 275, 315, 320, 616, 677, 707, 917, 1045
Hin6I GCGC 4 cut(s) 205, 207, 867, 947
HinP1I GCGC 4 cut(s) 205, 207, 867, 947
HincII GTYRAC 1 cut(s) 808
HindII GTYRAC 1 cut(s) 808
HindIII AAGCTT 1 cut(s) 493
HinfI GANTC 2 cut(s) 68, 829
HpaI GTTAAC 1 cut(s) 808
HpaII CCGG 2 cut(s) 488, 587
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 5 cut(s) 686, 808, 877, 928, 1050
Hpy188I TCNGA 3 cut(s) 455, 568, 1067
Hpy188III TCNNGA 7 cut(s) 46, 197, 335, 363, 464, 533, 727
Hpy8I GTNNAC 5 cut(s) 686, 808, 877, 928, 1050
Hpy99I CGWCG 2 cut(s) 203, 311
HpyAV CCTTC 5 cut(s) 102, 115, 289, 343, 962
HpyCH4III ACNGT 3 cut(s) 778, 842, 874
HpyCH4V TGCA 5 cut(s) 8, 98, 815, 826, 862
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 23
HpyF3I CTNAG 3 cut(s) 147, 663, 789
Hsp92II CATG 8 cut(s) 275, 315, 320, 616, 677, 707, 917, 1045
HspAI GCGC 4 cut(s) 205, 207, 867, 947
Ksp22I TGATCA 2 cut(s) 19, 1057
KspAI GTTAAC 1 cut(s) 808
Kzo9I GATC 8 cut(s) 19, 133, 223, 303, 616, 700, 933, 1057
LmnI GCTCC 1 cut(s) 583
LweI GCATC 3 cut(s) 60, 849, 871
MaeI CTAG 4 cut(s) 62, 410, 635, 1072
MaeIII GTNAC 1 cut(s) 712
MalI GATC 8 cut(s) 21, 135, 225, 305, 618, 702, 935, 1059
MboI GATC 8 cut(s) 19, 133, 223, 303, 616, 700, 933, 1057
MboII GAAGA 8 cut(s) 170, 247, 254, 323, 358, 436, 463, 537
MhlI GDGCHC 1 cut(s) 759
MluCI AATT 3 cut(s) 234, 264, 353
MlyI GAGTC 1 cut(s) 62
MmeI TCCRAC 1 cut(s) 561
MnlI CCTC 8 cut(s) 196, 334, 429, 488, 663, 672, 731, 885
Mph1103I ATGCAT 1 cut(s) 864
MroXI GAANNNNTTC 2 cut(s) 353, 797
MseI TTAA 3 cut(s) 267, 290, 807
MspI CCGG 2 cut(s) 488, 587
MspR9I CCNGG 1 cut(s) 29
MvaI CCWGG 1 cut(s) 29
MvnI CGCG 5 cut(s) 207, 209, 222, 621, 867
MwoI GCNNNNNNNGC 2 cut(s) 14, 23
NcoI CCATGG 1 cut(s) 271
NdeI CATATG 1 cut(s) 100
NdeII GATC 8 cut(s) 19, 133, 223, 303, 616, 700, 933, 1057
NheI GCTAGC 1 cut(s) 634
NlaIII CATG 8 cut(s) 275, 315, 320, 616, 677, 707, 917, 1045
NlaIV GGNNCC 1 cut(s) 585
NmuCI GTSAC 1 cut(s) 712
NsiI ATGCAT 1 cut(s) 864
NspI RCATGY 2 cut(s) 315, 677
PaeR7I CTCGAG 1 cut(s) 46
PauI GCGCGC 1 cut(s) 205
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PdmI GAANNNNTTC 2 cut(s) 353, 797
PfeI GAWTC 1 cut(s) 829
PkrI GCNGC 2 cut(s) 7, 623
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
PshAI GACNNNNGTC 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 27
PspGI CCWGG 1 cut(s) 27
PspN4I GGNNCC 1 cut(s) 585
PspPI GGNCC 3 cut(s) 451, 785, 917
PstI CTGCAG 1 cut(s) 10
PteI GCGCGC 1 cut(s) 205
RsaI GTAC 3 cut(s) 279, 426, 848
RsaNI GTAC 3 cut(s) 278, 425, 847
SaqAI TTAA 3 cut(s) 267, 290, 807
SatI GCNGC 2 cut(s) 6, 622
Sau3AI GATC 8 cut(s) 19, 133, 223, 303, 616, 700, 933, 1057
Sau96I GGNCC 3 cut(s) 451, 785, 917
ScaI AGTACT 1 cut(s) 426
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 1 cut(s) 29
SduI GDGCHC 1 cut(s) 759
SfaNI GCATC 3 cut(s) 60, 849, 871
SfcI CTRYAG 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 46
SinI GGWCC 2 cut(s) 451, 917
SlaI CTCGAG 1 cut(s) 46
SmlI CTYRAG 2 cut(s) 46, 377
SmoI CTYRAG 2 cut(s) 46, 377
Sse9I AATT 3 cut(s) 234, 264, 353
SsiI CCGC 3 cut(s) 220, 621, 638
SspMI CTAG 4 cut(s) 62, 410, 635, 1072
StyD4I CCNGG 1 cut(s) 27
StyI CCWWGG 2 cut(s) 271, 543
TaaI ACNGT 3 cut(s) 778, 842, 874
TaqI TCGA 4 cut(s) 47, 168, 306, 532
TasI AATT 3 cut(s) 234, 264, 353
TatI WGTACW 2 cut(s) 424, 846
TauI GCSGC 1 cut(s) 624
TfiI GAWTC 1 cut(s) 829
Tru1I TTAA 3 cut(s) 267, 290, 807
Tru9I TTAA 3 cut(s) 267, 290, 807
TscAI CASTG 2 cut(s) 505, 781
TseFI GTSAC 1 cut(s) 712
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 712
TspDTI ATGAA 7 cut(s) 141, 247, 308, 623, 752, 1010, 1058
TspGWI ACGGA 1 cut(s) 913
TspRI CASTG 2 cut(s) 505, 781
VpaK11BI GGWCC 2 cut(s) 451, 917
XapI RAATTY 2 cut(s) 234, 353
XceI RCATGY 2 cut(s) 315, 677
XhoI CTCGAG 1 cut(s) 46
XmiI GTMKAC 2 cut(s) 685, 927
XmnI GAANNNNTTC 2 cut(s) 353, 797
XspI CTAG 4 cut(s) 62, 410, 635, 1072
ZrmI AGTACT 1 cut(s) 426
Zsp2I ATGCAT 1 cut(s) 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.