FvH4_5g33280

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000481
Physical Location & Seq
Forward (+)
24067563 .. 24069515
1953 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g33280.t1

Sequence Viewer

Length: 1047 bp
ATGGCCGAAGTTGCTCAAGCCTTGGAGACCTCAAATGTCACAAGCATCAAATCACTAGCTGAATCACCAGCTCTCAACTCTGTTCCTTCTGCTTATGATGAAGCTGATCCTAATGATCCTGAGTTCGCTATTCCCATTGTTGATATGTCTCTTCTCACCTCTGGTTCTCCTGATGAGCACGCAAAGATCGTCCGCGATCTTGCCAAGATTTGTGAAGAATGGGGCTTTTTCATTGTCATTAACCACGGAGTAGAAGAGAGCCTAATGAAGGGGATGTTTGACGCATGTCATGGATTTTTCAGTCTTCCAGACGAGGAGAAGAAGGAGTTTAATTCAGGAAATGATGTGACTGAGATGTTCAAGTATGGGACAAGCTATGATCTTGCATTGGACAAAATACTTCTATGGAGGGACTTCATGAGGTTCCAAACACATCCCGAGTTCTACTCCCTCTACAAACCGGCTGGCTTCAGTGAGATTTCGCTCGAGTATAGCAAAAGAGCCCGAGAAGTAGCCTTGGAAATAACTCGAGCAATATCAGAAAGCTTGCTGTTGGAGCCAGAGTACATATACAACACAATGAACTTGGATCGCGGCTTACAAGTGCTAGCAGGGAACTACTACCCACCTTGTCCTCAGCCTGAACATGCCATTGGTTTACCCCCTCATACTGATCCTGGTCTATTCACTATGCTCATCCAGAATGACTTGAATGGCCTCCAAGTCGAGCACAATGGAAAATGGCTCTCTGTCACTGGCCCTAAGAATTGCTATTTCGTTAACCTTGCAGACCAAATGCAGATCCTTACAAACGGTAGGTACAAGAGTTCGATGCATCGCGCCACCGTGAAGAACAAAGCTACAAGGATAACGATACCTTTAGCCCATGGACCATCCATAGACACGATCATAGCACCAGCGCCAGAGTTGTGTGAAAGATATGGACAACAACCAAAGTACCTTGCCATGAACTACAACGAATACTTAGAACTTCAGTATAGTGGCAAGAGCTACATGAAGTCCAAATTTGATCACATCCGAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

349

Amino Acids

39.3

Weight (kDa)

5.13

Isoelectric Point (pI)

36.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 44 - 147 3.7e-25 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 201 - 295 7.3e-29 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 195, 594, 840
AciI CCGC 2 cut(s) 193, 594
AclWI GGATC 5 cut(s) 101, 110, 597, 668, 796
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 1025
AcuI CTGAAG 2 cut(s) 454, 977
AfaI GTAC 3 cut(s) 566, 821, 959
AfiI CCNNNNNNNGG 1 cut(s) 268
AgsI TTSAA 2 cut(s) 361, 712
AjnI CCWGG 1 cut(s) 676
AjuI GAANNNNNNNTTGG 2 cut(s) 636, 668
AluBI AGCT 7 cut(s) 59, 71, 104, 375, 546, 860, 1011
AluI AGCT 7 cut(s) 59, 71, 104, 375, 546, 860, 1011
Alw21I GWGCWC 2 cut(s) 180, 732
Alw26I GTCTC 2 cut(s) 20, 153
AlwI GGATC 5 cut(s) 101, 110, 597, 668, 796
Ama87I CYCGRG 4 cut(s) 437, 485, 504, 528
AoxI GGCC 3 cut(s) 3, 715, 757
ApoI RAATTY 1 cut(s) 1025
AspLEI GCGC 2 cut(s) 842, 922
AspS9I GGNCC 2 cut(s) 758, 890
AsuHPI GGTGA 2 cut(s) 57, 148
AsuNHI GCTAGC 1 cut(s) 607
AvaI CYCGRG 4 cut(s) 437, 485, 504, 528
AvaII GGWCC 1 cut(s) 890
BaeI ACNNNNGTAYC 4 cut(s) 811, 844, 941, 974
BanII GRGCYC 1 cut(s) 505
BbsI GAAGAC 1 cut(s) 296
Bbv12I GWGCWC 2 cut(s) 180, 732
BbvCI CCTCAGC 1 cut(s) 636
BccI CCATC 1 cut(s) 901
BciT130I CCWGG 1 cut(s) 678
BclI TGATCA 1 cut(s) 1030
BcoDI GTCTC 2 cut(s) 20, 153
BfaI CTAG 3 cut(s) 56, 608, 1045
BfoI RGCGCY 1 cut(s) 923
BisI GCNGC 1 cut(s) 595
BlsI GCNGC 1 cut(s) 596
Bme1390I CCNGG 1 cut(s) 678
Bme18I GGWCC 1 cut(s) 890
BmeT110I CYCGRG 4 cut(s) 437, 485, 504, 528
BmgT120I GGNCC 2 cut(s) 758, 890
BmiI GGNNCC 2 cut(s) 425, 558
BmrFI CCNGG 1 cut(s) 678
BmsI GCATC 3 cut(s) 54, 822, 844
BmtI GCTAGC 1 cut(s) 611
BoxI GACNNNNGTC 1 cut(s) 285
BpiI GAAGAC 1 cut(s) 296
BplI GAGNNNNNCTC 2 cut(s) 431, 463
Bpu10I CCTNAGC 1 cut(s) 636
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 4 cut(s) 21, 244, 516, 886
BsaXI ACNNNNNCTCC 2 cut(s) 548, 578
Bsc4I CCNNNNNNNGG 1 cut(s) 268
Bse118I RCCGGY 1 cut(s) 460
Bse1I ACTGG 1 cut(s) 760
BseBI CCWGG 1 cut(s) 678
BseDI CCNNGG 4 cut(s) 21, 244, 516, 886
BseGI GGATG 5 cut(s) 279, 433, 696, 893, 1035
BseLI CCNNNNNNNGG 1 cut(s) 268
BseMII CTCAG 3 cut(s) 111, 342, 650
BseNI ACTGG 1 cut(s) 760
BseRI GAGGAG 1 cut(s) 329
Bsh1236I CGCG 3 cut(s) 195, 594, 840
BshFI GGCC 3 cut(s) 5, 717, 759
BsiHKAI GWGCWC 2 cut(s) 180, 732
BsiHKCI CYCGRG 4 cut(s) 437, 485, 504, 528
BsiSI CCGG 1 cut(s) 461
BslFI GGGAC 2 cut(s) 382, 425
BslI CCNNNNNNNGG 1 cut(s) 268
BsmAI GTCTC 2 cut(s) 20, 153
BsmFI GGGAC 2 cut(s) 382, 425
BsnI GGCC 3 cut(s) 5, 717, 759
Bso31I GGTCTC 1 cut(s) 20
BsoBI CYCGRG 4 cut(s) 437, 485, 504, 528
Bsp1286I GDGCHC 3 cut(s) 180, 505, 732
Bsp19I CCATGG 1 cut(s) 886
BspACI CCGC 2 cut(s) 193, 594
BspANI GGCC 3 cut(s) 5, 717, 759
BspCNI CTCAG 3 cut(s) 112, 343, 649
BspFNI CGCG 3 cut(s) 195, 594, 840
BspHI TCATGA 1 cut(s) 417
BspLI GGNNCC 2 cut(s) 425, 558
BspOI GCTAGC 1 cut(s) 611
BspPI GGATC 5 cut(s) 101, 110, 597, 668, 796
BspTNI GGTCTC 1 cut(s) 20
BsrFI RCCGGY 1 cut(s) 460
BsrI ACTGG 1 cut(s) 760
BssAI RCCGGY 1 cut(s) 460
BssECI CCNNGG 4 cut(s) 21, 244, 516, 886
BssT1I CCWWGG 3 cut(s) 21, 516, 886
Bst2UI CCWGG 1 cut(s) 678
Bst4CI ACNGT 2 cut(s) 815, 847
Bst6I CTCTTC 2 cut(s) 156, 249
BstC8I GCNNGC 4 cut(s) 180, 466, 548, 609
BstDEI CTNAG 5 cut(s) 120, 351, 636, 762, 985
BstDSI CCRYGG 2 cut(s) 244, 886
BstENI CCTNNNNNAGG 1 cut(s) 266
BstF5I GGATG 5 cut(s) 279, 433, 696, 893, 1035
BstFNI CGCG 3 cut(s) 195, 594, 840
BstH2I RGCGCY 1 cut(s) 923
BstHHI GCGC 2 cut(s) 842, 922
BstMAI GTCTC 2 cut(s) 20, 153
BstMWI GCNNNNNNNGC 2 cut(s) 11, 556
BstNI CCWGG 1 cut(s) 678
BstNSI RCATGY 2 cut(s) 288, 650
BstPAI GACNNNNGTC 1 cut(s) 285
BstSCI CCNGG 1 cut(s) 676
BstUI CGCG 3 cut(s) 195, 594, 840
BstV2I GAAGAC 1 cut(s) 296
BstX2I RGATCY 1 cut(s) 801
BstYI RGATCY 1 cut(s) 801
BsuRI GGCC 3 cut(s) 5, 717, 759
BtgI CCRYGG 2 cut(s) 244, 886
BtgZI GCGATG 1 cut(s) 821
BtsCI GGATG 5 cut(s) 279, 433, 696, 893, 1035
BtsIMutI CAGTG 2 cut(s) 478, 753
Cac8I GCNNGC 4 cut(s) 180, 466, 548, 609
CciI TCATGA 1 cut(s) 417
CfoI GCGC 2 cut(s) 842, 922
Cfr10I RCCGGY 1 cut(s) 460
Cfr13I GGNCC 2 cut(s) 758, 890
CseI GACGC 1 cut(s) 290
Csp6I GTAC 3 cut(s) 565, 820, 958
CviAII CATG 7 cut(s) 285, 290, 418, 647, 887, 967, 1015
CviQI GTAC 3 cut(s) 565, 820, 958
DdeI CTNAG 5 cut(s) 120, 351, 636, 762, 985
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 156, 249
EarI CTCTTC 2 cut(s) 156, 249
Eco130I CCWWGG 3 cut(s) 21, 516, 886
Eco24I GRGCYC 1 cut(s) 505
Eco31I GGTCTC 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 890
Eco57I CTGAAG 2 cut(s) 454, 977
Eco88I CYCGRG 4 cut(s) 437, 485, 504, 528
EcoNI CCTNNNNNAGG 1 cut(s) 266
EcoRII CCWGG 1 cut(s) 676
EcoT14I CCWWGG 3 cut(s) 21, 516, 886
EcoT22I ATGCAT 1 cut(s) 837
EcoT38I GRGCYC 1 cut(s) 505
ErhI CCWWGG 3 cut(s) 21, 516, 886
FaeI CATG 7 cut(s) 288, 293, 421, 650, 890, 970, 1018
FaqI GGGAC 2 cut(s) 382, 425
FatI CATG 7 cut(s) 284, 289, 417, 646, 886, 966, 1014
FbaI TGATCA 1 cut(s) 1030
Fnu4HI GCNGC 1 cut(s) 595
FokI GGATG 5 cut(s) 286, 420, 683, 880, 1022
FriOI GRGCYC 1 cut(s) 505
Fsp4HI GCNGC 1 cut(s) 595
FspBI CTAG 3 cut(s) 56, 608, 1045
GlaI GCGC 2 cut(s) 841, 921
GluI GCNGC 1 cut(s) 595
HaeII RGCGCY 1 cut(s) 923
HaeIII GGCC 3 cut(s) 5, 717, 759
HapII CCGG 1 cut(s) 461
HgaI GACGC 1 cut(s) 290
HhaI GCGC 2 cut(s) 842, 922
Hin1II CATG 7 cut(s) 288, 293, 421, 650, 890, 970, 1018
Hin6I GCGC 2 cut(s) 840, 920
HinP1I GCGC 2 cut(s) 840, 920
HincII GTYRAC 1 cut(s) 781
HindII GTYRAC 1 cut(s) 781
HindIII AAGCTT 1 cut(s) 544
HinfI GANTC 1 cut(s) 62
HpaI GTTAAC 1 cut(s) 781
HpaII CCGG 1 cut(s) 461
HphI GGTGA 2 cut(s) 57, 148
Hpy166II GTNNAC 2 cut(s) 659, 781
Hpy188I TCNGA 2 cut(s) 541, 1040
Hpy188III TCNNGA 7 cut(s) 119, 170, 308, 336, 418, 437, 700
Hpy8I GTNNAC 2 cut(s) 659, 781
HpyAV CCTTC 3 cut(s) 96, 262, 316
HpyCH4III ACNGT 2 cut(s) 815, 847
HpyCH4V TGCA 4 cut(s) 386, 788, 799, 835
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 556
HpyF3I CTNAG 5 cut(s) 120, 351, 636, 762, 985
Hsp92II CATG 7 cut(s) 288, 293, 421, 650, 890, 970, 1018
HspAI GCGC 2 cut(s) 840, 920
Ksp22I TGATCA 1 cut(s) 1030
KspAI GTTAAC 1 cut(s) 781
LmnI GCTCC 1 cut(s) 556
LweI GCATC 3 cut(s) 54, 822, 844
MaeI CTAG 3 cut(s) 56, 608, 1045
MaeIII GTNAC 3 cut(s) 37, 346, 751
MboII GAAGA 6 cut(s) 143, 227, 266, 296, 331, 862
MflI RGATCY 1 cut(s) 801
MhlI GDGCHC 3 cut(s) 180, 505, 732
MluCI AATT 3 cut(s) 331, 766, 1025
MmeI TCCRAC 1 cut(s) 534
MnlI CCTC 9 cut(s) 40, 169, 307, 402, 414, 461, 645, 675, 728
Mph1103I ATGCAT 1 cut(s) 837
MseI TTAA 3 cut(s) 240, 330, 780
MspI CCGG 1 cut(s) 461
MspR9I CCNGG 1 cut(s) 678
MvaI CCWGG 1 cut(s) 678
MvnI CGCG 3 cut(s) 195, 594, 840
MwoI GCNNNNNNNGC 2 cut(s) 11, 556
NcoI CCATGG 1 cut(s) 886
NheI GCTAGC 1 cut(s) 607
NlaIII CATG 7 cut(s) 288, 293, 421, 650, 890, 970, 1018
NlaIV GGNNCC 2 cut(s) 425, 558
NmuCI GTSAC 3 cut(s) 37, 346, 751
NsiI ATGCAT 1 cut(s) 837
NspI RCATGY 2 cut(s) 288, 650
PaeR7I CTCGAG 2 cut(s) 485, 528
PagI TCATGA 1 cut(s) 417
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PfeI GAWTC 1 cut(s) 62
PkrI GCNGC 1 cut(s) 596
PshAI GACNNNNGTC 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 676
PspGI CCWGG 1 cut(s) 676
PspN4I GGNNCC 2 cut(s) 425, 558
PspPI GGNCC 2 cut(s) 758, 890
PspXI VCTCGAGB 2 cut(s) 485, 528
PsuI RGATCY 1 cut(s) 801
RsaI GTAC 3 cut(s) 566, 821, 959
RsaNI GTAC 3 cut(s) 565, 820, 958
SaqAI TTAA 3 cut(s) 240, 330, 780
SatI GCNGC 1 cut(s) 595
Sau96I GGNCC 2 cut(s) 758, 890
ScrFI CCNGG 1 cut(s) 678
SduI GDGCHC 3 cut(s) 180, 505, 732
SfaNI GCATC 3 cut(s) 54, 822, 844
Sfr274I CTCGAG 2 cut(s) 485, 528
SinI GGWCC 1 cut(s) 890
SlaI CTCGAG 2 cut(s) 485, 528
SmlI CTYRAG 3 cut(s) 15, 485, 528
SmoI CTYRAG 3 cut(s) 15, 485, 528
Sse9I AATT 3 cut(s) 331, 766, 1025
SsiI CCGC 2 cut(s) 193, 594
SspMI CTAG 3 cut(s) 56, 608, 1045
StyD4I CCNGG 1 cut(s) 676
StyI CCWWGG 3 cut(s) 21, 516, 886
TaaI ACNGT 2 cut(s) 815, 847
TaqI TCGA 4 cut(s) 486, 529, 726, 830
TasI AATT 3 cut(s) 331, 766, 1025
TatI WGTACW 1 cut(s) 564
TauI GCSGC 1 cut(s) 597
TfiI GAWTC 1 cut(s) 62
Tru1I TTAA 3 cut(s) 240, 330, 780
Tru9I TTAA 3 cut(s) 240, 330, 780
TscAI CASTG 2 cut(s) 478, 760
TseFI GTSAC 3 cut(s) 37, 346, 751
Tsp45I GTSAC 3 cut(s) 37, 346, 751
TspDTI ATGAA 7 cut(s) 114, 220, 281, 406, 596, 983, 1031
TspGWI ACGGA 1 cut(s) 261
TspRI CASTG 2 cut(s) 478, 760
VpaK11BI GGWCC 1 cut(s) 890
XagI CCTNNNNNAGG 1 cut(s) 266
XapI RAATTY 1 cut(s) 1025
XceI RCATGY 2 cut(s) 288, 650
XhoI CTCGAG 2 cut(s) 485, 528
XspI CTAG 3 cut(s) 56, 608, 1045
Zsp2I ATGCAT 1 cut(s) 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.