pycom11g13270

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000481
Physical Location & Seq
Reverse (-)
12432336 .. 12433665
1330 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g13270.2

Sequence Viewer

Length: 1062 bp
ATGGCTCAAGTTGCCGCTCAAACCATGGATGTAAAAAGCATCAAATCGCTTGCTGAATCACCTGCTCTCAGCTCTGTTCCTTCTGCTTATGCCTTCAACATGAACCCAAATGACGAAGCAGATCCAAACGATCCCGAATTCGCAATCCCCATAGTTGATATGTCTCTTCTTACCTCCGAATCTCCTGATCAACGGTCCAAAATTATCCAAGACCTCATCAAAATTTGCCAAGAATGGGGTTTCTTCATTGCGATTAACCACGGAGTCCCAGAGAGCCTAATGAAGGCGATGATTGATGCGTGCCATGGATTTTTCAGCCTCCCAGACGAAGAGAAGGAGGAGTTCAAATCCGGAAACGATGTGCTGGAGATGTTCAAGTACGGAACCAGCTACAATCTTGCATTGGACAAGGTTCTTCTCTGGAGGGACTTCTTCAAGGTGAGAGTACATCCTGAGTTCTACTCCCTCTACAAACCGGCTTGCTTCAGTGAGGTCTCAATGGAGTTCAGCAAGAGAACAAGAGAAGTAGCATTGGAGATAATACGCGCAATATCAGAAAGCTTGGGATTGGAGCCCAACTACATACACGACGCGATGAACATGGATCGCGGCATACAAATGCTCGCGGCCAACTACTATCCGCCTTGCCCTCAGCCAGAACATGCAATTGGTATACCACATCAGATCGATCATGGCCTTGTCACTCTGCTCATCCAGAATGAGATGAATGGCCTCCAGGTTGAACACAACGGCAAATGGCTCACCGTCAATGGCCCTGCCAACGGTTTCTTTGTCAACCTAGCTGACCAAATGCAAATTCTTACTAATGGAAAATACAAGAGTGTGATGCATCGGGCCACGGTGAACAACAAAGCTACAAGGATATCTATTGCCGTACCACATGGACCATCTGTTGATACAATTATCGCACCGGCGCCGGAGTTGCTGGAAAAAGAAGGCCAAGCTCCGAAATACATTGGAATGAACTATAAGGAGTATATACAGCTTCAGCAAAGCAGCAAGAACTACATGAAATCCACCTTTGATCATATCCTAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

354

Amino Acids

39.66

Weight (kDa)

5.31

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 70
Acc36I ACCTGC 1 cut(s) 70
AccB1I GGYRCC 1 cut(s) 934
AccBSI CCGCTC 1 cut(s) 17
AccI GTMKAC 1 cut(s) 673
AccII CGCG 4 cut(s) 546, 593, 609, 626
AccIII TCCGGA 1 cut(s) 350
AciI CCGC 4 cut(s) 15, 609, 626, 641
AclWI GGATC 3 cut(s) 116, 125, 612
AcoI YGGCCR 1 cut(s) 627
AcsI RAATTY 3 cut(s) 137, 222, 816
AcuI CTGAAG 2 cut(s) 469, 992
AcyI GRCGYC 1 cut(s) 935
AfaI GTAC 3 cut(s) 380, 447, 897
AfiI CCNNNNNNNGG 3 cut(s) 235, 283, 782
AgsI TTSAA 5 cut(s) 97, 346, 376, 436, 743
AjnI CCWGG 1 cut(s) 735
AjuI GAANNNNNNNTTGG 4 cut(s) 651, 683, 801, 833
AluBI AGCT 7 cut(s) 72, 390, 561, 803, 875, 965, 1006
AluI AGCT 7 cut(s) 72, 390, 561, 803, 875, 965, 1006
Alw26I GTCTC 2 cut(s) 168, 499
AlwI GGATC 3 cut(s) 116, 125, 612
Aor13HI TCCGGA 1 cut(s) 350
AoxI GGCC 6 cut(s) 627, 694, 730, 772, 855, 958
ApeKI GCWGC 1 cut(s) 1017
ApoI RAATTY 3 cut(s) 137, 222, 816
AspLEI GCGC 2 cut(s) 548, 937
AspS9I GGNCC 4 cut(s) 195, 773, 855, 905
AsuHPI GGTGA 4 cut(s) 51, 451, 754, 874
AvaII GGWCC 2 cut(s) 195, 905
BanI GGYRCC 1 cut(s) 934
BanII GRGCYC 1 cut(s) 576
BbvCI CCTCAGC 1 cut(s) 651
BbvI GCAGC 1 cut(s) 1029
BccI CCATC 1 cut(s) 916
BceAI ACGGC 2 cut(s) 766, 878
BciT130I CCWGG 1 cut(s) 737
BclI TGATCA 2 cut(s) 187, 1045
BcoDI GTCTC 2 cut(s) 168, 499
BfaI CTAG 2 cut(s) 800, 1055
BfoI RGCGCY 1 cut(s) 938
BfuAI ACCTGC 1 cut(s) 70
BisI GCNGC 4 cut(s) 15, 610, 627, 1018
BlsI GCNGC 4 cut(s) 16, 611, 628, 1019
Bme1390I CCNGG 1 cut(s) 737
Bme18I GGWCC 2 cut(s) 195, 905
BmgT120I GGNCC 4 cut(s) 195, 773, 855, 905
BmiI GGNNCC 3 cut(s) 385, 573, 936
BmrFI CCNGG 1 cut(s) 737
BmsI GCATC 4 cut(s) 48, 286, 837, 859
BplI GAGNNNNNCTC 2 cut(s) 446, 478
BpmI CTGGAG 3 cut(s) 386, 442, 719
Bpu10I CCTNAGC 1 cut(s) 651
Bsa29I ATCGAT 1 cut(s) 687
BsaHI GRCGYC 1 cut(s) 935
BsaI GGTCTC 1 cut(s) 499
BsaJI CCNNGG 4 cut(s) 24, 259, 304, 858
BsaWI WCCGGW 1 cut(s) 350
Bsc4I CCNNNNNNNGG 3 cut(s) 235, 283, 782
Bse118I RCCGGY 2 cut(s) 475, 931
Bse3DI GCAATG 1 cut(s) 246
BseAI TCCGGA 1 cut(s) 350
BseBI CCWGG 1 cut(s) 737
BseCI ATCGAT 1 cut(s) 687
BseDI CCNNGG 4 cut(s) 24, 259, 304, 858
BseGI GGATG 3 cut(s) 34, 448, 711
BseLI CCNNNNNNNGG 3 cut(s) 235, 283, 782
BseMI GCAATG 1 cut(s) 246
BseMII CTCAG 3 cut(s) 82, 444, 665
BseRI GAGGAG 1 cut(s) 353
BseXI GCAGC 1 cut(s) 1029
Bsh1236I CGCG 4 cut(s) 546, 593, 609, 626
BshFI GGCC 6 cut(s) 629, 696, 732, 774, 857, 960
BshNI GGYRCC 1 cut(s) 934
BshVI ATCGAT 1 cut(s) 687
BsiSI CCGG 4 cut(s) 351, 476, 932, 938
BslFI GGGAC 2 cut(s) 251, 440
BslI CCNNNNNNNGG 3 cut(s) 235, 283, 782
BsmAI GTCTC 2 cut(s) 168, 499
BsmFI GGGAC 2 cut(s) 251, 440
BsnI GGCC 6 cut(s) 629, 696, 732, 774, 857, 960
Bso31I GGTCTC 1 cut(s) 499
Bsp1286I GDGCHC 1 cut(s) 576
Bsp13I TCCGGA 1 cut(s) 350
Bsp143I GATC 7 cut(s) 121, 130, 187, 604, 684, 688, 1045
Bsp19I CCATGG 2 cut(s) 24, 304
BspACI CCGC 4 cut(s) 15, 609, 626, 641
BspANI GGCC 6 cut(s) 629, 696, 732, 774, 857, 960
BspCNI CTCAG 3 cut(s) 81, 445, 664
BspDI ATCGAT 1 cut(s) 687
BspEI TCCGGA 1 cut(s) 350
BspFNI CGCG 4 cut(s) 546, 593, 609, 626
BspLI GGNNCC 3 cut(s) 385, 573, 936
BspMI ACCTGC 1 cut(s) 70
BspPI GGATC 3 cut(s) 116, 125, 612
BspT107I GGYRCC 1 cut(s) 934
BspTNI GGTCTC 1 cut(s) 499
BsrBI CCGCTC 1 cut(s) 17
BsrDI GCAATG 1 cut(s) 246
BsrFI RCCGGY 2 cut(s) 475, 931
BssAI RCCGGY 2 cut(s) 475, 931
BssECI CCNNGG 4 cut(s) 24, 259, 304, 858
BssMI GATC 7 cut(s) 121, 130, 187, 604, 684, 688, 1045
BssNAI GTATAC 1 cut(s) 674
BssNI GRCGYC 1 cut(s) 935
BssT1I CCWWGG 2 cut(s) 24, 304
Bst1107I GTATAC 1 cut(s) 674
Bst2UI CCWGG 1 cut(s) 737
Bst4CI ACNGT 4 cut(s) 195, 766, 785, 862
Bst6I CTCTTC 2 cut(s) 171, 324
BstACI GRCGYC 1 cut(s) 935
BstC8I GCNNGC 4 cut(s) 51, 301, 481, 624
BstDEI CTNAG 3 cut(s) 68, 453, 651
BstDSI CCRYGG 4 cut(s) 24, 259, 304, 858
BstENI CCTNNNNNAGG 1 cut(s) 281
BstF5I GGATG 3 cut(s) 34, 448, 711
BstFNI CGCG 4 cut(s) 546, 593, 609, 626
BstH2I RGCGCY 1 cut(s) 938
BstHHI GCGC 2 cut(s) 548, 937
BstKTI GATC 7 cut(s) 124, 133, 190, 607, 687, 691, 1048
BstMAI GTCTC 2 cut(s) 168, 499
BstMBI GATC 7 cut(s) 121, 130, 187, 604, 684, 688, 1045
BstMWI GCNNNNNNNGC 2 cut(s) 11, 943
BstNI CCWGG 1 cut(s) 737
BstNSI RCATGY 1 cut(s) 665
BstSCI CCNGG 1 cut(s) 735
BstUI CGCG 4 cut(s) 546, 593, 609, 626
BstV1I GCAGC 1 cut(s) 1029
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
BstZ17I GTATAC 1 cut(s) 674
Bsu15I ATCGAT 1 cut(s) 687
BsuRI GGCC 6 cut(s) 629, 696, 732, 774, 857, 960
BsuTUI ATCGAT 1 cut(s) 687
BtgI CCRYGG 4 cut(s) 24, 259, 304, 858
BtgZI GCGATG 2 cut(s) 302, 608
BtsCI GGATG 3 cut(s) 34, 448, 711
BtsIMutI CAGTG 1 cut(s) 493
BveI ACCTGC 1 cut(s) 70
Cac8I GCNNGC 4 cut(s) 51, 301, 481, 624
CfoI GCGC 2 cut(s) 548, 937
Cfr10I RCCGGY 2 cut(s) 475, 931
Cfr13I GGNCC 4 cut(s) 195, 773, 855, 905
ClaI ATCGAT 1 cut(s) 687
CseI GACGC 1 cut(s) 599
Csp6I GTAC 3 cut(s) 379, 446, 896
CviAII CATG 8 cut(s) 25, 100, 305, 601, 662, 692, 902, 1030
CviQI GTAC 3 cut(s) 379, 446, 896
DdeI CTNAG 3 cut(s) 68, 453, 651
DinI GGCGCC 1 cut(s) 936
DpnI GATC 7 cut(s) 123, 132, 189, 606, 686, 690, 1047
DpnII GATC 7 cut(s) 121, 130, 187, 604, 684, 688, 1045
EaeI YGGCCR 1 cut(s) 627
Eam1104I CTCTTC 2 cut(s) 171, 324
EarI CTCTTC 2 cut(s) 171, 324
EciI GGCGGA 1 cut(s) 630
Eco130I CCWWGG 2 cut(s) 24, 304
Eco24I GRGCYC 1 cut(s) 576
Eco31I GGTCTC 1 cut(s) 499
Eco32I GATATC 1 cut(s) 885
Eco47I GGWCC 2 cut(s) 195, 905
Eco57I CTGAAG 2 cut(s) 469, 992
EcoNI CCTNNNNNAGG 1 cut(s) 281
EcoRI GAATTC 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 735
EcoRV GATATC 1 cut(s) 885
EcoT14I CCWWGG 2 cut(s) 24, 304
EcoT22I ATGCAT 1 cut(s) 852
EcoT38I GRGCYC 1 cut(s) 576
EgeI GGCGCC 1 cut(s) 936
EheI GGCGCC 1 cut(s) 936
ErhI CCWWGG 2 cut(s) 24, 304
FaeI CATG 8 cut(s) 28, 103, 308, 604, 665, 695, 905, 1033
FaqI GGGAC 2 cut(s) 251, 440
FatI CATG 8 cut(s) 24, 99, 304, 600, 661, 691, 901, 1029
FbaI TGATCA 2 cut(s) 187, 1045
FblI GTMKAC 1 cut(s) 673
Fnu4HI GCNGC 4 cut(s) 15, 610, 627, 1018
FokI GGATG 3 cut(s) 41, 435, 698
FriOI GRGCYC 1 cut(s) 576
Fsp4HI GCNGC 4 cut(s) 15, 610, 627, 1018
FspBI CTAG 2 cut(s) 800, 1055
GlaI GCGC 2 cut(s) 547, 936
GluI GCNGC 4 cut(s) 15, 610, 627, 1018
GsuI CTGGAG 3 cut(s) 386, 442, 719
HaeII RGCGCY 1 cut(s) 938
HaeIII GGCC 6 cut(s) 629, 696, 732, 774, 857, 960
HapII CCGG 4 cut(s) 351, 476, 932, 938
HgaI GACGC 1 cut(s) 599
HhaI GCGC 2 cut(s) 548, 937
Hin1I GRCGYC 1 cut(s) 935
Hin1II CATG 8 cut(s) 28, 103, 308, 604, 665, 695, 905, 1033
Hin6I GCGC 2 cut(s) 546, 935
HinP1I GCGC 2 cut(s) 546, 935
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HindIII AAGCTT 1 cut(s) 559
HinfI GANTC 3 cut(s) 56, 179, 264
HpaII CCGG 4 cut(s) 351, 476, 932, 938
HphI GGTGA 4 cut(s) 51, 451, 754, 874
Hpy166II GTNNAC 3 cut(s) 674, 796, 865
Hpy188I TCNGA 4 cut(s) 178, 556, 684, 969
Hpy188III TCNNGA 6 cut(s) 134, 185, 351, 421, 452, 715
Hpy8I GTNNAC 3 cut(s) 674, 796, 865
Hpy99I CGWCG 1 cut(s) 593
HpyAV CCTTC 5 cut(s) 90, 103, 277, 328, 950
HpyCH4III ACNGT 4 cut(s) 195, 766, 785, 862
HpyCH4V TGCA 4 cut(s) 401, 665, 814, 850
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 943
HpyF3I CTNAG 3 cut(s) 68, 453, 651
Hsp92I GRCGYC 1 cut(s) 935
Hsp92II CATG 8 cut(s) 28, 103, 308, 604, 665, 695, 905, 1033
HspAI GCGC 2 cut(s) 546, 935
KasI GGCGCC 1 cut(s) 934
Kpn2I TCCGGA 1 cut(s) 350
Ksp22I TGATCA 2 cut(s) 187, 1045
Kzo9I GATC 7 cut(s) 121, 130, 187, 604, 684, 688, 1045
LmnI GCTCC 2 cut(s) 571, 970
Lsp1109I GCAGC 1 cut(s) 1029
LweI GCATC 4 cut(s) 48, 286, 837, 859
MaeI CTAG 2 cut(s) 800, 1055
MaeIII GTNAC 1 cut(s) 700
MalI GATC 7 cut(s) 123, 132, 189, 606, 686, 690, 1047
MbiI CCGCTC 1 cut(s) 17
MboI GATC 7 cut(s) 121, 130, 187, 604, 684, 688, 1045
MboII GAAGA 5 cut(s) 158, 235, 341, 407, 424
MfeI CAATTG 1 cut(s) 666
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 1 cut(s) 576
MluCI AATT 6 cut(s) 137, 201, 222, 666, 816, 921
Mly113I GGCGCC 1 cut(s) 935
MlyI GAGTC 1 cut(s) 273
MnlI CCTC 9 cut(s) 184, 224, 329, 331, 417, 476, 484, 660, 743
Mph1103I ATGCAT 1 cut(s) 852
MroI TCCGGA 1 cut(s) 350
MseI TTAA 1 cut(s) 255
MslI CAYNNNNRTG 2 cut(s) 617, 980
MspI CCGG 4 cut(s) 351, 476, 932, 938
MspR9I CCNGG 1 cut(s) 737
MunI CAATTG 1 cut(s) 666
MvaI CCWGG 1 cut(s) 737
MvnI CGCG 4 cut(s) 546, 593, 609, 626
MwoI GCNNNNNNNGC 2 cut(s) 11, 943
NarI GGCGCC 1 cut(s) 935
NcoI CCATGG 2 cut(s) 24, 304
NdeII GATC 7 cut(s) 121, 130, 187, 604, 684, 688, 1045
NlaIII CATG 8 cut(s) 28, 103, 308, 604, 665, 695, 905, 1033
NlaIV GGNNCC 3 cut(s) 385, 573, 936
NmuCI GTSAC 1 cut(s) 700
NsiI ATGCAT 1 cut(s) 852
NspI RCATGY 1 cut(s) 665
PaqCI CACCTGC 1 cut(s) 70
PfeI GAWTC 2 cut(s) 56, 179
PkrI GCNGC 4 cut(s) 16, 611, 628, 1019
PleI GAGTC 1 cut(s) 272
PluTI GGCGCC 1 cut(s) 938
PpsI GAGTC 1 cut(s) 272
Psp6I CCWGG 1 cut(s) 735
PspGI CCWGG 1 cut(s) 735
PspN4I GGNNCC 3 cut(s) 385, 573, 936
PspPI GGNCC 4 cut(s) 195, 773, 855, 905
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 3 cut(s) 380, 447, 897
RsaNI GTAC 3 cut(s) 379, 446, 896
RseI CAYNNNNRTG 2 cut(s) 617, 980
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 4 cut(s) 15, 610, 627, 1018
Sau3AI GATC 7 cut(s) 121, 130, 187, 604, 684, 688, 1045
Sau96I GGNCC 4 cut(s) 195, 773, 855, 905
SchI GAGTC 1 cut(s) 273
ScrFI CCNGG 1 cut(s) 737
SduI GDGCHC 1 cut(s) 576
SfaNI GCATC 4 cut(s) 48, 286, 837, 859
SfoI GGCGCC 1 cut(s) 936
SgrAI CRCCGGYG 1 cut(s) 931
SinI GGWCC 2 cut(s) 195, 905
SmiMI CAYNNNNRTG 2 cut(s) 617, 980
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
Sse9I AATT 6 cut(s) 137, 201, 222, 666, 816, 921
SsiI CCGC 4 cut(s) 15, 609, 626, 641
SspDI GGCGCC 1 cut(s) 934
SspMI CTAG 2 cut(s) 800, 1055
StyD4I CCNGG 1 cut(s) 735
StyI CCWWGG 2 cut(s) 24, 304
TaaI ACNGT 4 cut(s) 195, 766, 785, 862
TaqI TCGA 1 cut(s) 687
TasI AATT 6 cut(s) 137, 201, 222, 666, 816, 921
TatI WGTACW 1 cut(s) 445
TauI GCSGC 3 cut(s) 17, 612, 629
TfiI GAWTC 2 cut(s) 56, 179
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 1 cut(s) 493
TseFI GTSAC 1 cut(s) 700
TseI GCWGC 1 cut(s) 1017
Tsp45I GTSAC 1 cut(s) 700
TspDTI ATGAA 7 cut(s) 116, 235, 296, 611, 740, 998, 1046
TspGWI ACGGA 2 cut(s) 276, 396
TspRI CASTG 1 cut(s) 493
VpaK11BI GGWCC 2 cut(s) 195, 905
XagI CCTNNNNNAGG 1 cut(s) 281
XapI RAATTY 3 cut(s) 137, 222, 816
XceI RCATGY 1 cut(s) 665
XmiI GTMKAC 1 cut(s) 673
XspI CTAG 2 cut(s) 800, 1055
Zsp2I ATGCAT 1 cut(s) 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.