Rmu_sc0008518.1_g000022

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008518.1
OG0000481
Physical Location & Seq
Forward (+)
86724 .. 87828
1105 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008518.1_g000022.1.cds

Sequence Viewer

Length: 726 bp
atggctgcagttgctcaagcccaggagacatcaaatgtcacaagcatcaaatcactagctgagtcatcagctctcaactatgtgccttctgcatatgccttcaacataaaccccaataatgaagctgatcctaatgaccctgagtttgctattcccattgttgatatgtctcttctcacctctggttctcctgacgagcgtgcaaaaatcgttggcgatcttgtcaaaatttgtgaagaatggggcttcttcattgcaattaaccatggagtaccagagagcctaatgaaggcgatgattgacgcatgtcatggatttttcagtcttccagacgaggagaagaaggagtttaaatcaggaaatgatgttcttgagatgttcaatgaggtttcgctggagtttagcaaaagaagccgagaagtagccttgcaaataacaagagcaatatcggaaagcttgggtttggggccggactacatatacaacacaatgaacatggatcgcggcttacaaatgctagcggggaactactacccaccttgtcctcagcctgaacatgccattggtataccccatcataccgatcatggtctagtcacactcctcattcagaatgagatgaatggtctccaagttgagcacaatggaaaatggctcactgtcaatggccctcccaatggctttttcgtcaaccttgctgatcaaatgcaggtactagctggttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

241

Amino Acids

26.45

Weight (kDa)

4.62

Isoelectric Point (pI)

31.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 700
AccI GTMKAC 1 cut(s) 568
AccII CGCG 1 cut(s) 504
AciI CCGC 2 cut(s) 504, 521
AclWI GGATC 2 cut(s) 122, 507
AcsI RAATTY 1 cut(s) 228
AfaI GTAC 2 cut(s) 273, 714
AfiI CCNNNNNNNGG 2 cut(s) 289, 677
AgsI TTSAA 2 cut(s) 103, 382
AjnI CCWGG 1 cut(s) 21
AjuI GAANNNNNNNTTGG 4 cut(s) 546, 578, 668, 700
AloI GAACNNNNNNTCC 2 cut(s) 351, 383
AluBI AGCT 5 cut(s) 59, 71, 125, 456, 719
AluI AGCT 5 cut(s) 59, 71, 125, 456, 719
Alw21I GWGCWC 1 cut(s) 642
Alw26I GTCTC 3 cut(s) 20, 174, 632
AlwI GGATC 2 cut(s) 122, 507
AoxI GGCC 2 cut(s) 467, 667
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 228
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AspS9I GGNCC 2 cut(s) 467, 668
AsuHPI GGTGA 1 cut(s) 169
AsuNHI GCTAGC 1 cut(s) 517
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 1 cut(s) 642
BbvCI CCTCAGC 1 cut(s) 546
BccI CCATC 1 cut(s) 582
BciT130I CCWGG 1 cut(s) 23
BclI TGATCA 1 cut(s) 700
BcoDI GTCTC 3 cut(s) 20, 174, 632
BfaI CTAG 4 cut(s) 56, 518, 593, 716
BfmI CTRYAG 1 cut(s) 6
BfuAI ACCTGC 1 cut(s) 700
BisI GCNGC 2 cut(s) 6, 505
BlsI GCNGC 2 cut(s) 7, 506
Bme1390I CCNGG 1 cut(s) 23
BmgT120I GGNCC 2 cut(s) 467, 668
BmiI GGNNCC 1 cut(s) 468
BmrFI CCNGG 1 cut(s) 23
BmsI GCATC 1 cut(s) 54
BmtI GCTAGC 1 cut(s) 521
BoxI GACNNNNGTC 1 cut(s) 306
BpiI GAAGAC 1 cut(s) 317
BpmI CTGGAG 1 cut(s) 416
Bpu10I CCTNAGC 1 cut(s) 546
BpuEI CTTGAG 1 cut(s) 392
BsaI GGTCTC 1 cut(s) 632
BsaJI CCNNGG 2 cut(s) 21, 265
Bsc4I CCNNNNNNNGG 2 cut(s) 289, 677
Bse3DI GCAATG 1 cut(s) 252
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 2 cut(s) 21, 265
BseLI CCNNNNNNNGG 2 cut(s) 289, 677
BseMI GCAATG 1 cut(s) 252
BseMII CTCAG 3 cut(s) 51, 132, 560
BseRI GAGGAG 2 cut(s) 350, 593
Bsh1236I CGCG 1 cut(s) 504
BshFI GGCC 2 cut(s) 469, 669
BsiHKAI GWGCWC 1 cut(s) 642
BsiSI CCGG 1 cut(s) 470
BslI CCNNNNNNNGG 2 cut(s) 289, 677
BsmAI GTCTC 3 cut(s) 20, 174, 632
BsnI GGCC 2 cut(s) 469, 669
Bso31I GGTCTC 1 cut(s) 632
Bsp1286I GDGCHC 1 cut(s) 642
Bsp143I GATC 5 cut(s) 127, 217, 499, 583, 700
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 2 cut(s) 504, 521
BspANI GGCC 2 cut(s) 469, 669
BspCNI CTCAG 3 cut(s) 52, 133, 559
BspFNI CGCG 1 cut(s) 504
BspLI GGNNCC 1 cut(s) 468
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 1 cut(s) 700
BspOI GCTAGC 1 cut(s) 521
BspPI GGATC 2 cut(s) 122, 507
BspTNI GGTCTC 1 cut(s) 632
BsrDI GCAATG 1 cut(s) 252
BssECI CCNNGG 2 cut(s) 21, 265
BssMI GATC 5 cut(s) 127, 217, 499, 583, 700
BssNAI GTATAC 1 cut(s) 569
BssT1I CCWWGG 1 cut(s) 265
Bst1107I GTATAC 1 cut(s) 569
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 1 cut(s) 661
Bst6I CTCTTC 1 cut(s) 177
BstC8I GCNNGC 2 cut(s) 201, 519
BstDEI CTNAG 3 cut(s) 60, 141, 546
BstDSI CCRYGG 1 cut(s) 265
BstENI CCTNNNNNAGG 1 cut(s) 287
BstFNI CGCG 1 cut(s) 504
BstKTI GATC 5 cut(s) 130, 220, 502, 586, 703
BstMAI GTCTC 3 cut(s) 20, 174, 632
BstMBI GATC 5 cut(s) 127, 217, 499, 583, 700
BstMWI GCNNNNNNNGC 2 cut(s) 11, 411
BstNI CCWGG 1 cut(s) 23
BstNSI RCATGY 2 cut(s) 309, 560
BstPAI GACNNNNGTC 1 cut(s) 306
BstSCI CCNGG 1 cut(s) 21
BstSFI CTRYAG 1 cut(s) 6
BstUI CGCG 1 cut(s) 504
BstV2I GAAGAC 1 cut(s) 317
BstZ17I GTATAC 1 cut(s) 569
BsuRI GGCC 2 cut(s) 469, 669
BtgI CCRYGG 1 cut(s) 265
BtgZI GCGATG 1 cut(s) 308
BtsIMutI CAGTG 1 cut(s) 657
BveI ACCTGC 1 cut(s) 700
Cac8I GCNNGC 2 cut(s) 201, 519
Cfr13I GGNCC 2 cut(s) 467, 668
CseI GACGC 1 cut(s) 311
Csp6I GTAC 2 cut(s) 272, 713
CviAII CATG 6 cut(s) 266, 306, 311, 496, 557, 587
CviQI GTAC 2 cut(s) 272, 713
DdeI CTNAG 3 cut(s) 60, 141, 546
DpnI GATC 5 cut(s) 129, 219, 501, 585, 702
DpnII GATC 5 cut(s) 127, 217, 499, 583, 700
DraI TTTAAA 1 cut(s) 352
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Eco130I CCWWGG 1 cut(s) 265
Eco31I GGTCTC 1 cut(s) 632
EcoNI CCTNNNNNAGG 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 6 cut(s) 269, 309, 314, 499, 560, 590
FatI CATG 6 cut(s) 265, 305, 310, 495, 556, 586
FauI CCCGC 1 cut(s) 514
FauNDI CATATG 1 cut(s) 94
FbaI TGATCA 1 cut(s) 700
FblI GTMKAC 1 cut(s) 568
Fnu4HI GCNGC 2 cut(s) 6, 505
Fsp4HI GCNGC 2 cut(s) 6, 505
FspBI CTAG 4 cut(s) 56, 518, 593, 716
GluI GCNGC 2 cut(s) 6, 505
GsuI CTGGAG 1 cut(s) 416
HaeIII GGCC 2 cut(s) 469, 669
HapII CCGG 1 cut(s) 470
HgaI GACGC 1 cut(s) 311
Hin1II CATG 6 cut(s) 269, 309, 314, 499, 560, 590
HincII GTYRAC 1 cut(s) 691
HindII GTYRAC 1 cut(s) 691
HindIII AAGCTT 1 cut(s) 454
HinfI GANTC 1 cut(s) 62
HpaII CCGG 1 cut(s) 470
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 2 cut(s) 569, 691
Hpy188I TCNGA 2 cut(s) 451, 612
Hpy188III TCNNGA 4 cut(s) 191, 329, 357, 371
Hpy8I GTNNAC 2 cut(s) 569, 691
HpyAV CCTTC 4 cut(s) 96, 109, 283, 337
HpyCH4III ACNGT 1 cut(s) 661
HpyCH4V TGCA 6 cut(s) 8, 92, 203, 257, 430, 709
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 411
HpyF3I CTNAG 3 cut(s) 60, 141, 546
Hsp92II CATG 6 cut(s) 269, 309, 314, 499, 560, 590
Ksp22I TGATCA 1 cut(s) 700
Kzo9I GATC 5 cut(s) 127, 217, 499, 583, 700
LweI GCATC 1 cut(s) 54
MaeI CTAG 4 cut(s) 56, 518, 593, 716
MaeIII GTNAC 2 cut(s) 37, 595
MalI GATC 5 cut(s) 129, 219, 501, 585, 702
MboI GATC 5 cut(s) 127, 217, 499, 583, 700
MboII GAAGA 5 cut(s) 164, 241, 248, 317, 352
MhlI GDGCHC 1 cut(s) 642
MluCI AATT 2 cut(s) 228, 258
MlyI GAGTC 1 cut(s) 71
MnlI CCTC 6 cut(s) 190, 328, 379, 555, 614, 681
MseI TTAA 3 cut(s) 261, 351, 724
MspI CCGG 1 cut(s) 470
MspR9I CCNGG 1 cut(s) 23
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 1 cut(s) 504
MwoI GCNNNNNNNGC 2 cut(s) 11, 411
NcoI CCATGG 1 cut(s) 265
NdeI CATATG 1 cut(s) 94
NdeII GATC 5 cut(s) 127, 217, 499, 583, 700
NheI GCTAGC 1 cut(s) 517
NlaIII CATG 6 cut(s) 269, 309, 314, 499, 560, 590
NlaIV GGNNCC 1 cut(s) 468
NmeAIII GCCGAG 1 cut(s) 440
NmuCI GTSAC 2 cut(s) 37, 595
NspI RCATGY 2 cut(s) 309, 560
PkrI GCNGC 2 cut(s) 7, 506
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PshAI GACNNNNGTC 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 1 cut(s) 468
PspPI GGNCC 2 cut(s) 467, 668
PstI CTGCAG 1 cut(s) 10
RsaI GTAC 2 cut(s) 273, 714
RsaNI GTAC 2 cut(s) 272, 713
SaqAI TTAA 3 cut(s) 261, 351, 724
SatI GCNGC 2 cut(s) 6, 505
Sau3AI GATC 5 cut(s) 127, 217, 499, 583, 700
Sau96I GGNCC 2 cut(s) 467, 668
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 1 cut(s) 23
SduI GDGCHC 1 cut(s) 642
SfaNI GCATC 1 cut(s) 54
SfcI CTRYAG 1 cut(s) 6
SmlI CTYRAG 2 cut(s) 15, 371
SmoI CTYRAG 2 cut(s) 15, 371
Sse9I AATT 2 cut(s) 228, 258
SsiI CCGC 2 cut(s) 504, 521
SspMI CTAG 4 cut(s) 56, 518, 593, 716
StyD4I CCNGG 1 cut(s) 21
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 1 cut(s) 661
TasI AATT 2 cut(s) 228, 258
TauI GCSGC 1 cut(s) 507
Tru1I TTAA 3 cut(s) 261, 351, 724
Tru9I TTAA 3 cut(s) 261, 351, 724
TscAI CASTG 1 cut(s) 664
TseFI GTSAC 2 cut(s) 37, 595
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 2 cut(s) 37, 595
TspDTI ATGAA 5 cut(s) 135, 241, 302, 506, 635
TspRI CASTG 1 cut(s) 664
XagI CCTNNNNNAGG 1 cut(s) 287
XapI RAATTY 1 cut(s) 228
XceI RCATGY 2 cut(s) 309, 560
XmiI GTMKAC 1 cut(s) 568
XspI CTAG 4 cut(s) 56, 518, 593, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.