Rh6BG183200

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000481
Physical Location & Seq
Forward (+)
33603405 .. 33605471
2067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG183200.1

Sequence Viewer

Length: 879 bp
ATGGCTGCAGTTGCTCAGGCCTTAGAGACCCCCTCATCTGTTACCAGCATCAAATCACTAGCTGAGTCCTCTGCTCTAAACACTGTCCCTTCAGCCTATGCCTTCAACATAAACCCCAATGACGAAGCAGATCCAAAAGATCCCGAATTCGCAATTCCTATCATCGATATGTCACTTCTCACATCTGGTACTCCTGAACAACGCTCCCAAATCATCCATGACCTCGTCAAGATTTGTCAAGAATGGGGCTTCTTCATAGCAATTAACCATGGAGTACCAGAGAGCCTAATGAAGGCGATGATTGACGCATGTCATGGCTTCTTCAGTCTTCCGGACGAGGAGAAGAAGGAATTCAAATCAGGGAATGATGTGCTAGAAATGTTCAAGTATGGTACCAGTTATAACCTTGCCTTGGATAAGGTCCTTCTTTGGAGGGACTTCTTCAAGGTCAGAACCCATCCTGAGTTTTACTCCCTCTACAAACCGGCTACCTTCAGTGAGGTTTCAATGGAGTTTGGCAAAAGAACAAGAGAAGTGGCACTAGAAATCACAAAAGCAATATCCGAAAGCCTGGGATTGGAGCCAAATTACATATACGAAAAGATGAACATGGATCGTGGCTTACAATTGTTGGCCGGGAACTACTATCCTCCTTGCCCTCAGCCGGAACATGCCATTGGTATACCACATCACACCGATCACGGTCTAGTCACACTCCTCATCCAGAATGAGATGAATGGCCTCCAAGTCGAACACAACGGAAAATGGCTCACCGTAAACGGCCCTGCCAATGGATTTTTTGTTAACCTTGCTGATCAAATGCAGGTACTAATTAACTACTTAATGGAAAACCAGATAAACACTTGTATTAAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

292

Amino Acids

32.77

Weight (kDa)

5.04

Isoelectric Point (pI)

31.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 52 - 156 4e-26 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 209 - 279 4.6e-17 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g20420 FvH4_5g20421 FvH4_5g20430 FvH4_5g20431 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g20432 FvH4_5g33280
malus_domestica MD03G1140300.v1.1 MD03G1140400.v1.1 MD11G1171500.v1.1
prunus_persica Prupe.8G112300_v2.0.a1
pyrus_communis pycom03g09520 pycom11g13270
rosa_chinensis RchiOBHm_Chr2g0142431 RchiOBHm_Chr5g0068661 RchiOBHm_Chr6g0271401 RchiOBHm_Chr7g0205561 RchiOBHm_Chr7g0205581 RchiOBHm_Chr7g0205721 RchiOBHm_Chr7g0205741
rosa_laevigata RLG00000003357 RLG00000003358 RLG00000003359 RLG00000003363 RLG00000009087 RLG00000019958 RLG00000035989
rosa_multiflora Rmu_sc0000076.1_g000053 Rmu_sc0000076.1_g000054 Rmu_sc0000076.1_g000055 Rmu_sc0001211.1_g000022 Rmu_sc0001679.1_g000017 Rmu_sc0008518.1_g000018 Rmu_sc0008518.1_g000022 Rmu_sc0008518.1_g000024 Rmu_ssc0000482.1_g000004
rosa_roxburghii Rroxscaffold_1G00012230 Rroxscaffold_2G00102620 Rroxscaffold_3G00252010 Rroxscaffold_3G00252020 Rroxscaffold_3G00252030 Rroxscaffold_3G00252050 Rroxscaffold_7G00197030
rosa_rugosa Rorug02G0372500 Rorug02G0372600 Rorug06G0060800 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092600 Rorug07G0092700 Rorug07G0092800
rosa_samantha Rh2AG424300 Rh2BG433900 Rh2CG410900 Rh2DG443900 Rh5AG449900 Rh5DG481500 Rh5DG481700 Rh6AG180000 Rh6BG183200 Rh6CG179900 Rh6DG171700 Rh7AG222800 Rh7AG223900 Rh7AG224200 Rh7AG224300 Rh7BG219100 Rh7BG219200 Rh7BG219300 Rh7BG219500 Rh7CG235600 Rh7CG237400 Rh7CG237700 Rh7CG237800 Rh7DG229400 Rh7DG229600 Rh7DG231000
rosa_wichuraiana Rw2G034750 Rw5G042000 Rw6G015460 Rw7G019220 Rw7G019310 Rw7G019320 Rw7G019330 Rw7G019340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 402
Acc36I ACCTGC 1 cut(s) 814
Acc65I GGTACC 1 cut(s) 392
AccB1I GGYRCC 1 cut(s) 392
AccI GTMKAC 1 cut(s) 682
AccIII TCCGGA 1 cut(s) 331
AclWI GGATC 3 cut(s) 125, 134, 621
AcoI YGGCCR 1 cut(s) 633
AcsI RAATTY 2 cut(s) 146, 350
AcuI CTGAAG 3 cut(s) 75, 307, 478
AfaI GTAC 4 cut(s) 190, 276, 394, 828
AfiI CCNNNNNNNGG 5 cut(s) 292, 412, 577, 664, 791
AgsI TTSAA 5 cut(s) 106, 355, 385, 445, 507
AjnI CCWGG 1 cut(s) 570
AjuI GAANNNNNNNTTGG 2 cut(s) 660, 692
AluBI AGCT 1 cut(s) 62
AluI AGCT 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 20
AlwI GGATC 3 cut(s) 125, 134, 621
Aor13HI TCCGGA 1 cut(s) 331
AoxI GGCC 4 cut(s) 18, 633, 739, 781
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 2 cut(s) 146, 350
Asp700I GAANNNNTTC 1 cut(s) 350
Asp718I GGTACC 1 cut(s) 392
AspS9I GGNCC 2 cut(s) 421, 782
AsuC2I CCSGG 1 cut(s) 637
AsuHPI GGTGA 1 cut(s) 763
AvaII GGWCC 1 cut(s) 421
BanI GGYRCC 1 cut(s) 392
BbsI GAAGAC 1 cut(s) 320
BbvCI CCTCAGC 1 cut(s) 660
BccI CCATC 1 cut(s) 465
BceAI ACGGC 1 cut(s) 796
BciT130I CCWGG 1 cut(s) 572
BclI TGATCA 1 cut(s) 814
BcnI CCSGG 1 cut(s) 637
BcoDI GTCTC 1 cut(s) 20
BfaI CTAG 4 cut(s) 59, 374, 542, 707
BfmI CTRYAG 1 cut(s) 6
BfuAI ACCTGC 1 cut(s) 814
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 2 cut(s) 572, 637
Bme18I GGWCC 1 cut(s) 421
BmgT120I GGNCC 2 cut(s) 421, 782
BmiI GGNNCC 2 cut(s) 394, 582
BmrFI CCNGG 2 cut(s) 572, 637
BmsI GCATC 1 cut(s) 57
BoxI GACNNNNGTC 1 cut(s) 309
BpiI GAAGAC 1 cut(s) 320
BplI GAGNNNNNCTC 4 cut(s) 17, 49, 455, 487
Bpu10I CCTNAGC 2 cut(s) 15, 660
BpuMI CCSGG 1 cut(s) 637
Bsa29I ATCGAT 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 3 cut(s) 268, 411, 571
BsaWI WCCGGW 1 cut(s) 331
Bsc4I CCNNNNNNNGG 5 cut(s) 292, 412, 577, 664, 791
Bse118I RCCGGY 1 cut(s) 484
Bse1I ACTGG 1 cut(s) 396
BseAI TCCGGA 1 cut(s) 331
BseBI CCWGG 1 cut(s) 572
BseCI ATCGAT 1 cut(s) 165
BseDI CCNNGG 3 cut(s) 268, 411, 571
BseGI GGATG 3 cut(s) 213, 457, 720
BseLI CCNNNNNNNGG 5 cut(s) 292, 412, 577, 664, 791
BseMII CTCAG 4 cut(s) 29, 54, 453, 674
BseNI ACTGG 1 cut(s) 396
BseRI GAGGAG 2 cut(s) 353, 707
BshFI GGCC 4 cut(s) 20, 635, 741, 783
BshNI GGYRCC 1 cut(s) 392
BshVI ATCGAT 1 cut(s) 165
BsiSI CCGG 4 cut(s) 332, 485, 636, 665
BslFI GGGAC 2 cut(s) 71, 449
BslI CCNNNNNNNGG 5 cut(s) 292, 412, 577, 664, 791
BsmAI GTCTC 1 cut(s) 20
BsmFI GGGAC 2 cut(s) 71, 449
BsnI GGCC 4 cut(s) 20, 635, 741, 783
Bso31I GGTCTC 1 cut(s) 20
Bsp13I TCCGGA 1 cut(s) 331
Bsp143I GATC 5 cut(s) 130, 139, 613, 697, 814
Bsp19I CCATGG 1 cut(s) 268
BspANI GGCC 4 cut(s) 20, 635, 741, 783
BspCNI CTCAG 4 cut(s) 28, 55, 454, 673
BspDI ATCGAT 1 cut(s) 165
BspEI TCCGGA 1 cut(s) 331
BspLI GGNNCC 2 cut(s) 394, 582
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 1 cut(s) 814
BspPI GGATC 3 cut(s) 125, 134, 621
BspT107I GGYRCC 1 cut(s) 392
BspTNI GGTCTC 1 cut(s) 20
BsrFI RCCGGY 1 cut(s) 484
BsrI ACTGG 1 cut(s) 396
BssAI RCCGGY 1 cut(s) 484
BssECI CCNNGG 3 cut(s) 268, 411, 571
BssMI GATC 5 cut(s) 130, 139, 613, 697, 814
BssNAI GTATAC 1 cut(s) 683
BssT1I CCWWGG 2 cut(s) 268, 411
Bst1107I GTATAC 1 cut(s) 683
Bst2UI CCWGG 1 cut(s) 572
Bst4CI ACNGT 3 cut(s) 85, 704, 775
BstDEI CTNAG 5 cut(s) 15, 22, 63, 462, 660
BstDSI CCRYGG 1 cut(s) 268
BstENI CCTNNNNNAGG 1 cut(s) 290
BstF5I GGATG 3 cut(s) 213, 457, 720
BstKTI GATC 5 cut(s) 133, 142, 616, 700, 817
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 5 cut(s) 130, 139, 613, 697, 814
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 572
BstNSI RCATGY 2 cut(s) 312, 674
BstPAI GACNNNNGTC 1 cut(s) 309
BstSCI CCNGG 2 cut(s) 570, 635
BstSFI CTRYAG 1 cut(s) 6
BstV2I GAAGAC 1 cut(s) 320
BstX2I RGATCY 2 cut(s) 130, 139
BstYI RGATCY 2 cut(s) 130, 139
BstZ17I GTATAC 1 cut(s) 683
Bsu15I ATCGAT 1 cut(s) 165
BsuRI GGCC 4 cut(s) 20, 635, 741, 783
BsuTUI ATCGAT 1 cut(s) 165
BtgI CCRYGG 1 cut(s) 268
BtgZI GCGATG 1 cut(s) 311
BtsCI GGATG 3 cut(s) 213, 457, 720
BtsIMutI CAGTG 2 cut(s) 81, 502
BveI ACCTGC 1 cut(s) 814
Cfr10I RCCGGY 1 cut(s) 484
Cfr13I GGNCC 2 cut(s) 421, 782
ClaI ATCGAT 1 cut(s) 165
CseI GACGC 1 cut(s) 314
Csp6I GTAC 4 cut(s) 189, 275, 393, 827
CspCI CAANNNNNGTGG 2 cut(s) 516, 551
CviAII CATG 6 cut(s) 218, 269, 309, 314, 610, 671
CviQI GTAC 4 cut(s) 189, 275, 393, 827
DdeI CTNAG 5 cut(s) 15, 22, 63, 462, 660
DpnI GATC 5 cut(s) 132, 141, 615, 699, 816
DpnII GATC 5 cut(s) 130, 139, 613, 697, 814
EaeI YGGCCR 1 cut(s) 633
Eco130I CCWWGG 2 cut(s) 268, 411
Eco147I AGGCCT 1 cut(s) 20
Eco31I GGTCTC 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 421
Eco57I CTGAAG 3 cut(s) 75, 307, 478
EcoNI CCTNNNNNAGG 1 cut(s) 290
EcoO109I RGGNCCY 1 cut(s) 421
EcoRI GAATTC 2 cut(s) 146, 350
EcoRII CCWGG 1 cut(s) 570
EcoT14I CCWWGG 2 cut(s) 268, 411
ErhI CCWWGG 2 cut(s) 268, 411
FaeI CATG 6 cut(s) 221, 272, 312, 317, 613, 674
FaqI GGGAC 2 cut(s) 71, 449
FatI CATG 6 cut(s) 217, 268, 308, 313, 609, 670
FbaI TGATCA 1 cut(s) 814
FblI GTMKAC 1 cut(s) 682
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 3 cut(s) 200, 444, 707
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 4 cut(s) 59, 374, 542, 707
GluI GCNGC 1 cut(s) 6
HaeIII GGCC 4 cut(s) 20, 635, 741, 783
HapII CCGG 4 cut(s) 332, 485, 636, 665
HgaI GACGC 1 cut(s) 314
Hin1II CATG 6 cut(s) 221, 272, 312, 317, 613, 674
HincII GTYRAC 1 cut(s) 805
HindII GTYRAC 1 cut(s) 805
HinfI GANTC 1 cut(s) 65
HpaI GTTAAC 1 cut(s) 805
HpaII CCGG 4 cut(s) 332, 485, 636, 665
HphI GGTGA 1 cut(s) 763
Hpy166II GTNNAC 3 cut(s) 683, 778, 805
Hpy188I TCNGA 2 cut(s) 452, 565
Hpy188III TCNNGA 7 cut(s) 143, 194, 229, 239, 332, 461, 724
Hpy8I GTNNAC 3 cut(s) 683, 778, 805
HpyAV CCTTC 6 cut(s) 99, 112, 286, 340, 434, 502
HpyCH4III ACNGT 3 cut(s) 85, 704, 775
HpyCH4V TGCA 2 cut(s) 8, 823
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 5 cut(s) 15, 22, 63, 462, 660
Hsp92II CATG 6 cut(s) 221, 272, 312, 317, 613, 674
Kpn2I TCCGGA 1 cut(s) 331
KpnI GGTACC 1 cut(s) 396
Ksp22I TGATCA 1 cut(s) 814
KspAI GTTAAC 1 cut(s) 805
Kzo9I GATC 5 cut(s) 130, 139, 613, 697, 814
LmnI GCTCC 2 cut(s) 209, 580
LweI GCATC 1 cut(s) 57
MaeI CTAG 4 cut(s) 59, 374, 542, 707
MaeIII GTNAC 3 cut(s) 40, 171, 709
MalI GATC 5 cut(s) 132, 141, 615, 699, 816
MboI GATC 5 cut(s) 130, 139, 613, 697, 814
MboII GAAGA 5 cut(s) 244, 313, 320, 355, 433
MfeI CAATTG 1 cut(s) 626
MflI RGATCY 2 cut(s) 130, 139
MluCI AATT 7 cut(s) 146, 153, 261, 350, 586, 626, 831
MlyI GAGTC 1 cut(s) 74
MroI TCCGGA 1 cut(s) 331
MroXI GAANNNNTTC 1 cut(s) 350
MseI TTAA 5 cut(s) 264, 804, 834, 842, 870
MslI CAYNNNNRTG 1 cut(s) 167
MspI CCGG 4 cut(s) 332, 485, 636, 665
MspR9I CCNGG 2 cut(s) 572, 637
MunI CAATTG 1 cut(s) 626
MvaI CCWGG 1 cut(s) 572
MwoI GCNNNNNNNGC 1 cut(s) 11
NciI CCSGG 1 cut(s) 637
NcoI CCATGG 1 cut(s) 268
NdeII GATC 5 cut(s) 130, 139, 613, 697, 814
NlaIII CATG 6 cut(s) 221, 272, 312, 317, 613, 674
NlaIV GGNNCC 2 cut(s) 394, 582
NmuCI GTSAC 2 cut(s) 171, 709
NspI RCATGY 2 cut(s) 312, 674
PceI AGGCCT 1 cut(s) 20
PdmI GAANNNNTTC 1 cut(s) 350
PflFI GACNNNGTC 1 cut(s) 224
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
PpuMI RGGWCCY 1 cut(s) 421
PshAI GACNNNNGTC 1 cut(s) 309
PsiI TTATAA 1 cut(s) 402
Psp5II RGGWCCY 1 cut(s) 421
Psp6I CCWGG 1 cut(s) 570
PspGI CCWGG 1 cut(s) 570
PspN4I GGNNCC 2 cut(s) 394, 582
PspPI GGNCC 2 cut(s) 421, 782
PspPPI RGGWCCY 1 cut(s) 421
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 2 cut(s) 130, 139
PsyI GACNNNGTC 1 cut(s) 224
RsaI GTAC 4 cut(s) 190, 276, 394, 828
RsaNI GTAC 4 cut(s) 189, 275, 393, 827
RseI CAYNNNNRTG 1 cut(s) 167
SaqAI TTAA 5 cut(s) 264, 804, 834, 842, 870
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 5 cut(s) 130, 139, 613, 697, 814
Sau96I GGNCC 2 cut(s) 421, 782
SchI GAGTC 1 cut(s) 74
ScrFI CCNGG 2 cut(s) 572, 637
SetI ASST 9 cut(s) 64, 225, 408, 423, 450, 494, 504, 810, 828
SfaNI GCATC 1 cut(s) 57
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 1 cut(s) 421
SmiMI CAYNNNNRTG 1 cut(s) 167
Sse9I AATT 7 cut(s) 146, 153, 261, 350, 586, 626, 831
SseBI AGGCCT 1 cut(s) 20
SspMI CTAG 4 cut(s) 59, 374, 542, 707
StuI AGGCCT 1 cut(s) 20
StyD4I CCNGG 2 cut(s) 570, 635
StyI CCWWGG 2 cut(s) 268, 411
TaaI ACNGT 3 cut(s) 85, 704, 775
TaqI TCGA 2 cut(s) 165, 750
TasI AATT 7 cut(s) 146, 153, 261, 350, 586, 626, 831
Tru1I TTAA 5 cut(s) 264, 804, 834, 842, 870
Tru9I TTAA 5 cut(s) 264, 804, 834, 842, 870
TscAI CASTG 2 cut(s) 88, 502
TseFI GTSAC 2 cut(s) 171, 709
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 2 cut(s) 171, 709
TspDTI ATGAA 4 cut(s) 244, 305, 620, 749
TspGWI ACGGA 1 cut(s) 774
TspRI CASTG 2 cut(s) 88, 502
Tth111I GACNNNGTC 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 421
XagI CCTNNNNNAGG 1 cut(s) 290
XapI RAATTY 2 cut(s) 146, 350
XceI RCATGY 2 cut(s) 312, 674
XmiI GTMKAC 1 cut(s) 682
XmnI GAANNNNTTC 1 cut(s) 350
XspI CTAG 4 cut(s) 59, 374, 542, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.