FvH4_6g09001
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004586
Physical Location & Seq
Forward (+)
5310397 .. 5313616
3220 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g09001.t1

Sequence Viewer

Length: 1221 bp
ATGAGGTCCTATACTCCTATGTCCCTCTTCAAAGTTTACAGTGGCACCGAGGACCCAAGTTCAACTGGGAATTCAGAACTCTCAGATTGGCAAAATATTCAAGGCATTCATCTGATCTCCTCGCTTACGGACCCCAATGACGCTTCTGTGCTCCAGACCTTGAAAGTGTCCTGGAAAAACACGCCACCAAGCTGGGATAAGTCATCTGATCCCTGTGGTTTTCCATGGGAAGGAGTTCAGTGCACTGGTGCTAGGGTGACTTCATTGAGATTATCATCCATGGGCCTCAAAGGGGAACTCAGTAGCTACATAGAGGGACTCACTGAACTAAACTCCTTGGACCTGTCATTCAATCCTGGACTCGATGGTTCTCTTCCTCCACAATTAGGAGATCTCCAAAAGTTGAATATCCTGATCCTAGCTGGCTGCAGCTTCGTTGGCCCCATTCCAGAGGAGTTGGGAAATCTTGAGCAGCTAACGTTCTTGGCTCTGAATACAAATTACTTAACTGGTAAAATACCCCCCTCTTTGGGCAAGCTCTCCAAACTCTACTGGCTTGACGTGGCAGAGAACCGATTGAGCGGACCTCTACCAATTTCGACCTCTACTTCTCCAGGCTTGGACCTCTTGTTAAACGCTAAACATTTGCATTTGAACAAGAACCTGCTTTCAGGTTCCATCCCACTCAATCTTTTCAGCTCCGAAATGATATTGATACACGCGTTACTTGATGGAAACAATTTTTCTGGGAGTATTCCAATGACAGTAGGACTTGTTAAAACTCTTGAAGTGGTTCGGCTTGATAACAATACTCTGTCTGGGAGTGTCCCCTCAAACCTTAACAACCTTACAAATGTCAATGAACTAAATTTGGCCAACAACAATTTGGCAGGCCCCTTGCCTGACTTGACCGGAATGAATTCTCTCATTTATGTAGACCTTAGTAACAACTCATTTGACCCATCTGAAGCTCCGCTTTGGTTCTCCACCTTACCCGCACTAACAACTCTGGTTATGGAGTATGGATCACTTCAAGGGCCTGTGCCAGAAAAGCTTTTCAGCCTTTCAGAATTACAGCAAGTGAAACTGAAAAACAATGCCCTTAATGGTACATTAAACATGGGAGATAGCATCAGTCTACAACTAGAGCTCGTTGATCTCCAGAACAATCAGATTTCCCAGATCGTTGTGGAAGAAGAGTTCAAAGATGAATTGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 Pkinase_Tyr

Protein Analysis

407

Amino Acids

43.98

Weight (kDa)

4.47

Isoelectric Point (pI)

33.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 45 - 82 1e-05 Leucine rich repeat N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 672
AccB1I GGYRCC 1 cut(s) 44
AccBSI CCGCTC 1 cut(s) 582
AccI GTMKAC 2 cut(s) 936, 1138
AccII CGCG 1 cut(s) 722
AciI CCGC 3 cut(s) 582, 974, 996
AclI AACGTT 1 cut(s) 479
AclWI GGATC 3 cut(s) 203, 409, 1033
AcoI YGGCCR 1 cut(s) 873
AcsI RAATTY 3 cut(s) 70, 868, 919
AcuI CTGAAG 1 cut(s) 987
AfaI GTAC 1 cut(s) 1111
AfiI CCNNNNNNNGG 5 cut(s) 230, 292, 386, 529, 530
AflIII ACRYGT 1 cut(s) 720
AjiI CACGTC 1 cut(s) 562
AjnI CCWGG 3 cut(s) 170, 355, 613
AloI GAACNNNNNNTCC 2 cut(s) 1184, 1216
Alw21I GWGCWC 3 cut(s) 153, 245, 1152
Alw44I GTGCAC 1 cut(s) 241
AlwI GGATC 3 cut(s) 203, 409, 1033
AoxI GGCC 5 cut(s) 283, 439, 873, 892, 1037
ApaLI GTGCAC 1 cut(s) 241
ApeKI GCWGC 3 cut(s) 426, 429, 472
ApoI RAATTY 3 cut(s) 70, 868, 919
Asp700I GAANNNNTTC 3 cut(s) 234, 792, 919
AsuHPI GGTGA 1 cut(s) 268
AvaII GGWCC 6 cut(s) 6, 52, 130, 340, 584, 622
BaeGI GKGCMC 1 cut(s) 245
BaeI ACNNNNGTAYC 2 cut(s) 707, 740
BalI TGGCCA 1 cut(s) 875
BanI GGYRCC 1 cut(s) 44
BanII GRGCYC 1 cut(s) 1152
Bbv12I GWGCWC 3 cut(s) 153, 245, 1152
BbvI GCAGC 3 cut(s) 413, 441, 484
BccI CCATC 4 cut(s) 359, 686, 725, 970
BciT130I CCWGG 3 cut(s) 172, 357, 615
BfaI CTAG 3 cut(s) 252, 419, 1145
BfmI CTRYAG 1 cut(s) 427
BfuAI ACCTGC 1 cut(s) 672
BglII AGATCT 1 cut(s) 391
BisI GCNGC 3 cut(s) 427, 430, 473
BlsI GCNGC 3 cut(s) 428, 431, 474
Bme1390I CCNGG 3 cut(s) 172, 357, 615
Bme18I GGWCC 6 cut(s) 6, 52, 130, 340, 584, 622
BmgBI CACGTC 1 cut(s) 562
BmiI GGNNCC 6 cut(s) 46, 54, 132, 442, 676, 895
BmrFI CCNGG 3 cut(s) 172, 357, 615
BmrI ACTGGG 1 cut(s) 75
BmsI GCATC 1 cut(s) 1140
BmuI ACTGGG 1 cut(s) 75
BplI GAGNNNNNCTC 2 cut(s) 571, 603
BpmI CTGGAG 3 cut(s) 137, 597, 1145
BpuEI CTTGAG 1 cut(s) 488
BsaBI GATNNNNATC 1 cut(s) 274
BsaJI CCNNGG 4 cut(s) 48, 224, 279, 336
BsaWI WCCGGW 1 cut(s) 911
Bsc4I CCNNNNNNNGG 5 cut(s) 230, 292, 386, 529, 530
Bse1I ACTGG 4 cut(s) 70, 250, 514, 557
Bse8I GATNNNNATC 1 cut(s) 274
BseBI CCWGG 3 cut(s) 172, 357, 615
BseDI CCNNGG 4 cut(s) 48, 224, 279, 336
BseGI GGATG 2 cut(s) 275, 678
BseJI GATNNNNATC 1 cut(s) 274
BseLI CCNNNNNNNGG 5 cut(s) 230, 292, 386, 529, 530
BseMII CTCAG 2 cut(s) 96, 313
BseNI ACTGG 4 cut(s) 70, 250, 514, 557
BseRI GAGGAG 2 cut(s) 109, 467
BseSI GKGCMC 1 cut(s) 245
BseXI GCAGC 3 cut(s) 413, 441, 484
BseYI CCCAGC 1 cut(s) 192
Bsh1236I CGCG 1 cut(s) 722
BshFI GGCC 5 cut(s) 285, 441, 875, 894, 1039
BshNI GGYRCC 1 cut(s) 44
BsiHKAI GWGCWC 3 cut(s) 153, 245, 1152
BsiSI CCGG 1 cut(s) 912
BslFI GGGAC 3 cut(s) 7, 330, 812
BslI CCNNNNNNNGG 5 cut(s) 230, 292, 386, 529, 530
BsmFI GGGAC 3 cut(s) 7, 330, 812
BsmI GAATGC 1 cut(s) 105
BsnI GGCC 5 cut(s) 285, 441, 875, 894, 1039
Bsp1286I GDGCHC 3 cut(s) 153, 245, 1152
Bsp143I GATC 7 cut(s) 114, 208, 391, 414, 1025, 1156, 1182
Bsp19I CCATGG 2 cut(s) 224, 279
BspACI CCGC 3 cut(s) 582, 974, 996
BspANI GGCC 5 cut(s) 285, 441, 875, 894, 1039
BspCNI CTCAG 2 cut(s) 95, 312
BspFNI CGCG 1 cut(s) 722
BspLI GGNNCC 6 cut(s) 46, 54, 132, 442, 676, 895
BspMAI CTGCAG 1 cut(s) 431
BspMI ACCTGC 1 cut(s) 672
BspPI GGATC 3 cut(s) 203, 409, 1033
BspT107I GGYRCC 1 cut(s) 44
BsrBI CCGCTC 1 cut(s) 582
BsrI ACTGG 4 cut(s) 70, 250, 514, 557
BssECI CCNNGG 4 cut(s) 48, 224, 279, 336
BssMI GATC 7 cut(s) 114, 208, 391, 414, 1025, 1156, 1182
BssT1I CCWWGG 3 cut(s) 224, 279, 336
Bst2UI CCWGG 3 cut(s) 172, 357, 615
Bst4CI ACNGT 2 cut(s) 41, 766
Bst6I CTCTTC 3 cut(s) 32, 378, 1191
BstC8I GCNNGC 3 cut(s) 424, 536, 892
BstDEI CTNAG 3 cut(s) 82, 299, 941
BstDSI CCRYGG 2 cut(s) 224, 279
BstF5I GGATG 2 cut(s) 275, 678
BstFNI CGCG 1 cut(s) 722
BstKTI GATC 7 cut(s) 117, 211, 394, 417, 1028, 1159, 1185
BstMBI GATC 7 cut(s) 114, 208, 391, 414, 1025, 1156, 1182
BstMWI GCNNNNNNNGC 2 cut(s) 438, 1051
BstNI CCWGG 3 cut(s) 172, 357, 615
BstSCI CCNGG 3 cut(s) 170, 355, 613
BstSFI CTRYAG 1 cut(s) 427
BstSLI GKGCMC 1 cut(s) 245
BstUI CGCG 1 cut(s) 722
BstV1I GCAGC 3 cut(s) 413, 441, 484
BstX2I RGATCY 1 cut(s) 391
BstXI CCANNNNNNTGG 1 cut(s) 192
BstYI RGATCY 1 cut(s) 391
BsuRI GGCC 5 cut(s) 285, 441, 875, 894, 1039
BtgI CCRYGG 2 cut(s) 224, 279
BtrI CACGTC 1 cut(s) 562
BtsCI GGATG 2 cut(s) 275, 678
BtsIMutI CAGTG 4 cut(s) 46, 243, 245, 321
BveI ACCTGC 1 cut(s) 672
Cac8I GCNNGC 3 cut(s) 424, 536, 892
CseI GACGC 1 cut(s) 149
Csp6I GTAC 1 cut(s) 1110
CviAII CATG 3 cut(s) 225, 280, 1120
CviQI GTAC 1 cut(s) 1110
DdeI CTNAG 3 cut(s) 82, 299, 941
DpnI GATC 7 cut(s) 116, 210, 393, 416, 1027, 1158, 1184
DpnII GATC 7 cut(s) 114, 208, 391, 414, 1025, 1156, 1182
EaeI YGGCCR 1 cut(s) 873
Eam1104I CTCTTC 3 cut(s) 32, 378, 1191
EarI CTCTTC 3 cut(s) 32, 378, 1191
Ecl136II GAGCTC 1 cut(s) 1150
Eco130I CCWWGG 3 cut(s) 224, 279, 336
Eco24I GRGCYC 1 cut(s) 1152
Eco47I GGWCC 6 cut(s) 6, 52, 130, 340, 584, 622
Eco53kI GAGCTC 1 cut(s) 1150
Eco57I CTGAAG 1 cut(s) 987
EcoICRI GAGCTC 1 cut(s) 1150
EcoO109I RGGNCCY 4 cut(s) 6, 52, 893, 1037
EcoRI GAATTC 2 cut(s) 70, 919
EcoRII CCWGG 3 cut(s) 170, 355, 613
EcoT14I CCWWGG 3 cut(s) 224, 279, 336
EcoT38I GRGCYC 1 cut(s) 1152
ErhI CCWWGG 3 cut(s) 224, 279, 336
FaeI CATG 3 cut(s) 228, 283, 1123
FaiI YATR 9 cut(s) 12, 20, 226, 281, 311, 933, 1016, 1023, 1121
FalI AAGNNNNNCTT 2 cut(s) 960, 992
FaqI GGGAC 3 cut(s) 7, 330, 812
FatI CATG 3 cut(s) 224, 279, 1119
FauI CCCGC 1 cut(s) 1003
FblI GTMKAC 2 cut(s) 936, 1138
Fnu4HI GCNGC 3 cut(s) 427, 430, 473
FokI GGATG 2 cut(s) 262, 665
FriOI GRGCYC 1 cut(s) 1152
Fsp4HI GCNGC 3 cut(s) 427, 430, 473
FspBI CTAG 3 cut(s) 252, 419, 1145
GluI GCNGC 3 cut(s) 427, 430, 473
GsaI CCCAGC 1 cut(s) 196
GsuI CTGGAG 3 cut(s) 137, 597, 1145
HaeIII GGCC 5 cut(s) 285, 441, 875, 894, 1039
HapII CCGG 1 cut(s) 912
HgaI GACGC 1 cut(s) 149
Hin1II CATG 3 cut(s) 228, 283, 1123
HindIII AAGCTT 1 cut(s) 1052
HinfI GANTC 2 cut(s) 318, 360
HpaII CCGG 1 cut(s) 912
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 4 cut(s) 37, 243, 937, 1139
Hpy188I TCNGA 9 cut(s) 76, 85, 114, 208, 492, 703, 967, 1069, 1173
Hpy188III TCNNGA 6 cut(s) 154, 412, 449, 467, 785, 1162
Hpy8I GTNNAC 4 cut(s) 37, 243, 937, 1139
HpyAV CCTTC 1 cut(s) 224
HpyCH4III ACNGT 2 cut(s) 41, 766
HpyCH4IV ACGT 2 cut(s) 479, 561
HpyCH4V TGCA 3 cut(s) 243, 429, 649
HpyF10VI GCNNNNNNNGC 2 cut(s) 438, 1051
HpyF3I CTNAG 3 cut(s) 82, 299, 941
HpySE526I ACGT 2 cut(s) 479, 561
Hsp92II CATG 3 cut(s) 228, 283, 1123
Kzo9I GATC 7 cut(s) 114, 208, 391, 414, 1025, 1156, 1182
LmnI GCTCC 3 cut(s) 156, 704, 976
Lsp1109I GCAGC 3 cut(s) 413, 441, 484
LweI GCATC 1 cut(s) 1140
MaeI CTAG 3 cut(s) 252, 419, 1145
MaeII ACGT 2 cut(s) 479, 561
MaeIII GTNAC 3 cut(s) 256, 723, 944
MalI GATC 7 cut(s) 116, 210, 393, 416, 1027, 1158, 1184
MbiI CCGCTC 1 cut(s) 582
MboI GATC 7 cut(s) 114, 208, 391, 414, 1025, 1156, 1182
MboII GAAGA 4 cut(s) 19, 365, 1205, 1208
MflI RGATCY 1 cut(s) 391
MhlI GDGCHC 3 cut(s) 153, 245, 1152
MlsI TGGCCA 1 cut(s) 875
MluI ACGCGT 1 cut(s) 720
MluNI TGGCCA 1 cut(s) 875
MlyI GAGTC 2 cut(s) 312, 354
Mox20I TGGCCA 1 cut(s) 875
MroXI GAANNNNTTC 3 cut(s) 234, 792, 919
MscI TGGCCA 1 cut(s) 875
MseI TTAA 6 cut(s) 506, 632, 777, 840, 1104, 1115
Msp20I TGGCCA 1 cut(s) 875
MspI CCGG 1 cut(s) 912
MspR9I CCNGG 3 cut(s) 172, 357, 615
Mva1269I GAATGC 1 cut(s) 105
MvaI CCWGG 3 cut(s) 172, 357, 615
MvnI CGCG 1 cut(s) 722
MwoI GCNNNNNNNGC 2 cut(s) 438, 1051
NcoI CCATGG 2 cut(s) 224, 279
NdeII GATC 7 cut(s) 114, 208, 391, 414, 1025, 1156, 1182
NlaIII CATG 3 cut(s) 228, 283, 1123
NlaIV GGNNCC 6 cut(s) 46, 54, 132, 442, 676, 895
NmuCI GTSAC 1 cut(s) 256
PctI GAATGC 1 cut(s) 105
PdmI GAANNNNTTC 3 cut(s) 234, 792, 919
PfoI TCCNGGA 2 cut(s) 170, 355
PkrI GCNGC 3 cut(s) 428, 431, 474
PleI GAGTC 2 cut(s) 312, 354
PpsI GAGTC 2 cut(s) 312, 354
PpuMI RGGWCCY 2 cut(s) 6, 52
Psp124BI GAGCTC 1 cut(s) 1152
Psp1406I AACGTT 1 cut(s) 479
Psp5II RGGWCCY 2 cut(s) 6, 52
Psp6I CCWGG 3 cut(s) 170, 355, 613
PspFI CCCAGC 1 cut(s) 192
PspGI CCWGG 3 cut(s) 170, 355, 613
PspN4I GGNNCC 6 cut(s) 46, 54, 132, 442, 676, 895
PspPPI RGGWCCY 2 cut(s) 6, 52
PstI CTGCAG 1 cut(s) 431
PsuI RGATCY 1 cut(s) 391
RsaI GTAC 1 cut(s) 1111
RsaNI GTAC 1 cut(s) 1110
SacI GAGCTC 1 cut(s) 1152
SaqAI TTAA 6 cut(s) 506, 632, 777, 840, 1104, 1115
SatI GCNGC 3 cut(s) 427, 430, 473
Sau3AI GATC 7 cut(s) 114, 208, 391, 414, 1025, 1156, 1182
SchI GAGTC 2 cut(s) 312, 354
ScrFI CCNGG 3 cut(s) 172, 357, 615
SduI GDGCHC 3 cut(s) 153, 245, 1152
SfaNI GCATC 1 cut(s) 1140
SfcI CTRYAG 1 cut(s) 427
SinI GGWCC 6 cut(s) 6, 52, 130, 340, 584, 622
SmlI CTYRAG 1 cut(s) 467
SmoI CTYRAG 1 cut(s) 467
SsiI CCGC 3 cut(s) 582, 974, 996
SspI AATATT 1 cut(s) 97
SspMI CTAG 3 cut(s) 252, 419, 1145
SstI GAGCTC 1 cut(s) 1152
StyD4I CCNGG 3 cut(s) 170, 355, 613
StyI CCWWGG 3 cut(s) 224, 279, 336
TaaI ACNGT 2 cut(s) 41, 766
TaiI ACGT 2 cut(s) 482, 564
TaqI TCGA 2 cut(s) 363, 599
Tru1I TTAA 6 cut(s) 506, 632, 777, 840, 1104, 1115
Tru9I TTAA 6 cut(s) 506, 632, 777, 840, 1104, 1115
TscAI CASTG 4 cut(s) 46, 245, 250, 328
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 3 cut(s) 426, 429, 472
Tsp45I GTSAC 1 cut(s) 256
TspDTI ATGAA 4 cut(s) 98, 252, 876, 932
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 4 cut(s) 46, 245, 250, 328
VneI GTGCAC 1 cut(s) 241
VpaK11BI GGWCC 6 cut(s) 6, 52, 130, 340, 584, 622
XapI RAATTY 3 cut(s) 70, 868, 919
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XmiI GTMKAC 2 cut(s) 936, 1138
XmnI GAANNNNTTC 3 cut(s) 234, 792, 919
XspI CTAG 3 cut(s) 252, 419, 1145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.