Rh3AG092500

GTPase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0004586
Physical Location & Seq
Forward (+)
7574258 .. 7581835
7578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG092500.1

Sequence Viewer

Length: 1194 bp
ATGATGATCTTGAGAGATTCATGGAAAAACACACCATCTAGCTGGGAACATTCTGATGATCCCTGTGGTGTGCCATGGGTAGGAGTCAACTGTACTGGTGCAAGGGTGACTGAATTGAGTTTATCAGCCATGGGGCTCATAGGGGAACTTTCCACCGGCTACATTGGGCAACTCGATGAACTAACGTCTTTGGATGTGTCATTCAATCCTGGCATCACAGGTCGTATTTCTCTACAAATATCATTTCTCCTGAAGTTAAATACCCTGAACCTAGCTGGATGCAGCTTCAGTGGTAGTATTCCAGATCAGTTGGGAGATATTGAACAGCTAACCTTCTTGGCTCTGAATTCAAATAACTTATCTGGAACAATACCTTCTGCTTTGGGTCAACTCTCCAATCTCTACTTGCTTGATCTATCAGACAATCAGCTGACTGGACCTCTCCCAATCTCAACCTCTACTTCTCCAGGAGGATTAGACCTCTTGTTAAAGGCTGAACACTTCCATTTGAGCAACAACCAGCTTTCAGGTTCCATTCCACCCGAGCTTTTCAGTTCTGACATGAGACTGATTCATCTGTTCCTTGATGGAAATAAACTTACCGGGGTTATTCCAATGACTTTAGGACTTGTTCAGACTTTTGAGATGGTTCGGCTTAATAGAAATACTCTGACCGGAAGTGTCCCCTCAAACCTAAACAACCTTACAAATGTCAATGAACTGAATTTGGCCAACAACAACTTGACAGGCCTTTTGCCAGACTTGACTGGAATGAATTCCCTCAATTATGTAGACCTTAGTAACAACTCATTTGACCCATCAGTAGCTCCACTTTGGTTCTCAACCTTACCCACACTTACCACTCTGGTTATGGAATTCGGATCACTTCAAGGACCTGTGCCAGAGAAGCTTTTCAGCCTTCCAGAATTACAGCAAGTGAAACTGAAATACAATACATTTAACGGTACACTGAACATGGGTGATAACATCAGTCTACAACTGGAGCTTGTGGATCTCCAGAACAATCAGATTTCCCATCTCACTATAGAAGAAGAATACAAGGATGCATTAATGTTTCGTCACAAACCTATAGTCTTGATGCGAATATCTGCCTTTCTTCGTCTCTATCAGTGCCATGCTTGCTGGCCCAAAATGGGCACCATTTGCTTCTTGGCTCACTGGCTGTTCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 ko04152 ko04921 map03008 map04152 map04921
Pfam Domains
Protein Families
EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

397

Amino Acids

43.61

Weight (kDa)

4.81

Isoelectric Point (pI)

29.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1157
AccI GTMKAC 2 cut(s) 792, 994
AclWI GGATC 3 cut(s) 53, 889, 1020
AcoI YGGCCR 1 cut(s) 729
AcsI RAATTY 4 cut(s) 346, 724, 775, 875
AcuI CTGAAG 2 cut(s) 271, 272
AfaI GTAC 2 cut(s) 94, 967
AfiI CCNNNNNNNGG 2 cut(s) 80, 1154
AgsI TTSAA 4 cut(s) 205, 323, 351, 890
AjnI CCWGG 2 cut(s) 208, 466
Alw26I GTCTC 2 cut(s) 559, 1127
AlwI GGATC 3 cut(s) 53, 889, 1020
Ama87I CYCGRG 1 cut(s) 542
AoxI GGCC 3 cut(s) 729, 748, 1145
ApeKI GCWGC 1 cut(s) 282
ApoI RAATTY 4 cut(s) 346, 724, 775, 875
ArsI GACNNNNNNTTYG 4 cut(s) 736, 768, 1077, 1109
AseI ATTAAT 1 cut(s) 1070
Asp700I GAANNNNTTC 2 cut(s) 775, 911
AspS9I GGNCC 3 cut(s) 437, 893, 1146
AsuC2I CCSGG 1 cut(s) 604
AsuHPI GGTGA 2 cut(s) 118, 992
AvaI CYCGRG 1 cut(s) 542
AvaII GGWCC 2 cut(s) 437, 893
BaeGI GKGCMC 1 cut(s) 1160
BalI TGGCCA 1 cut(s) 731
BanI GGYRCC 1 cut(s) 1157
BanII GRGCYC 1 cut(s) 138
BbvI GCAGC 1 cut(s) 294
BccI CCATC 5 cut(s) 43, 581, 640, 826, 1044
BciT130I CCWGG 2 cut(s) 210, 468
BcnI CCSGG 1 cut(s) 604
BcoDI GTCTC 2 cut(s) 559, 1127
BfaI CTAG 2 cut(s) 39, 272
BfmI CTRYAG 2 cut(s) 1044, 1089
BisI GCNGC 1 cut(s) 283
BlsI GCNGC 1 cut(s) 284
Bme1390I CCNGG 3 cut(s) 210, 468, 604
Bme18I GGWCC 2 cut(s) 437, 893
BmeT110I CYCGRG 1 cut(s) 542
BmgT120I GGNCC 3 cut(s) 437, 893, 1146
BmiI GGNNCC 2 cut(s) 532, 1159
BmrFI CCNGG 3 cut(s) 210, 468, 604
BmsI GCATC 4 cut(s) 222, 269, 1054, 1089
BpmI CTGGAG 3 cut(s) 450, 1001, 1022
BpuEI CTTGAG 1 cut(s) 31
BpuMI CCSGG 1 cut(s) 604
BsaJI CCNNGG 3 cut(s) 74, 129, 603
BsaWI WCCGGW 1 cut(s) 674
Bsc4I CCNNNNNNNGG 2 cut(s) 80, 1154
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 5 cut(s) 100, 439, 772, 1005, 1184
BseBI CCWGG 2 cut(s) 210, 468
BseDI CCNNGG 3 cut(s) 74, 129, 603
BseGI GGATG 3 cut(s) 199, 284, 1069
BseLI CCNNNNNNNGG 2 cut(s) 80, 1154
BseNI ACTGG 5 cut(s) 100, 439, 772, 1005, 1184
BseSI GKGCMC 1 cut(s) 1160
BseXI GCAGC 1 cut(s) 294
BseYI CCCAGC 1 cut(s) 42
BshFI GGCC 3 cut(s) 731, 750, 1147
BshNI GGYRCC 1 cut(s) 1157
BsiHKCI CYCGRG 1 cut(s) 542
BsiSI CCGG 3 cut(s) 156, 603, 675
BslFI GGGAC 1 cut(s) 668
BslI CCNNNNNNNGG 2 cut(s) 80, 1154
BsmAI GTCTC 2 cut(s) 559, 1127
BsmBI CGTCTC 1 cut(s) 1127
BsmFI GGGAC 1 cut(s) 668
BsnI GGCC 3 cut(s) 731, 750, 1147
BsoBI CYCGRG 1 cut(s) 542
Bsp1286I GDGCHC 2 cut(s) 138, 1160
Bsp143I GATC 6 cut(s) 6, 58, 304, 412, 881, 1012
Bsp19I CCATGG 2 cut(s) 74, 129
BspANI GGCC 3 cut(s) 731, 750, 1147
BspLI GGNNCC 2 cut(s) 532, 1159
BspPI GGATC 3 cut(s) 53, 889, 1020
BspT107I GGYRCC 1 cut(s) 1157
BsrFI RCCGGY 1 cut(s) 155
BsrI ACTGG 5 cut(s) 100, 439, 772, 1005, 1184
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 3 cut(s) 74, 129, 603
BssMI GATC 6 cut(s) 6, 58, 304, 412, 881, 1012
BssT1I CCWWGG 2 cut(s) 74, 129
Bst2UI CCWGG 2 cut(s) 210, 468
Bst4CI ACNGT 2 cut(s) 92, 965
BstAPI GCANNNNNTGC 1 cut(s) 1164
BstC8I GCNNGC 2 cut(s) 1141, 1145
BstDEI CTNAG 1 cut(s) 797
BstDSI CCRYGG 2 cut(s) 74, 129
BstF5I GGATG 3 cut(s) 199, 284, 1069
BstKTI GATC 6 cut(s) 9, 61, 307, 415, 884, 1015
BstMAI GTCTC 2 cut(s) 559, 1127
BstMBI GATC 6 cut(s) 6, 58, 304, 412, 881, 1012
BstMWI GCNNNNNNNGC 3 cut(s) 907, 1140, 1164
BstNI CCWGG 2 cut(s) 210, 468
BstSCI CCNGG 3 cut(s) 208, 466, 602
BstSFI CTRYAG 2 cut(s) 1044, 1089
BstSLI GKGCMC 1 cut(s) 1160
BstV1I GCAGC 1 cut(s) 294
BstX2I RGATCY 1 cut(s) 1012
BstXI CCANNNNNNTGG 1 cut(s) 42
BstYI RGATCY 1 cut(s) 1012
BsuRI GGCC 3 cut(s) 731, 750, 1147
BtgI CCRYGG 2 cut(s) 74, 129
BtsCI GGATG 3 cut(s) 199, 284, 1069
BtsIMutI CAGTG 4 cut(s) 295, 968, 1136, 1177
Cac8I GCNNGC 2 cut(s) 1141, 1145
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 3 cut(s) 437, 893, 1146
Csp6I GTAC 2 cut(s) 93, 966
CviAII CATG 6 cut(s) 21, 75, 130, 562, 976, 1136
CviQI GTAC 2 cut(s) 93, 966
DdeI CTNAG 1 cut(s) 797
DpnI GATC 6 cut(s) 8, 60, 306, 414, 883, 1014
DpnII GATC 6 cut(s) 6, 58, 304, 412, 881, 1012
EaeI YGGCCR 1 cut(s) 729
Eco130I CCWWGG 2 cut(s) 74, 129
Eco147I AGGCCT 1 cut(s) 750
Eco24I GRGCYC 1 cut(s) 138
Eco47I GGWCC 2 cut(s) 437, 893
Eco57I CTGAAG 2 cut(s) 271, 272
Eco88I CYCGRG 1 cut(s) 542
EcoO109I RGGNCCY 1 cut(s) 893
EcoRI GAATTC 3 cut(s) 346, 775, 875
EcoRII CCWGG 2 cut(s) 208, 466
EcoT14I CCWWGG 2 cut(s) 74, 129
EcoT22I ATGCAT 1 cut(s) 1069
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 2 cut(s) 74, 129
Esp3I CGTCTC 1 cut(s) 1127
FaeI CATG 6 cut(s) 24, 78, 133, 565, 979, 1139
FaqI GGGAC 1 cut(s) 668
FatI CATG 6 cut(s) 20, 74, 129, 561, 975, 1135
FblI GTMKAC 2 cut(s) 792, 994
Fnu4HI GCNGC 1 cut(s) 283
FokI GGATG 3 cut(s) 206, 291, 1076
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 1 cut(s) 283
FspBI CTAG 2 cut(s) 39, 272
GluI GCNGC 1 cut(s) 283
GsaI CCCAGC 1 cut(s) 46
GsuI CTGGAG 3 cut(s) 450, 1001, 1022
HaeIII GGCC 3 cut(s) 731, 750, 1147
HapII CCGG 3 cut(s) 156, 603, 675
Hin1II CATG 6 cut(s) 24, 78, 133, 565, 979, 1139
HincII GTYRAC 2 cut(s) 88, 389
HindII GTYRAC 2 cut(s) 88, 389
HindIII AAGCTT 1 cut(s) 908
HinfI GANTC 3 cut(s) 17, 84, 571
HpaII CCGG 3 cut(s) 156, 603, 675
HphI GGTGA 2 cut(s) 118, 992
Hpy166II GTNNAC 5 cut(s) 88, 389, 793, 968, 995
Hpy188I TCNGA 8 cut(s) 55, 345, 421, 559, 636, 672, 881, 1029
Hpy188III TCNNGA 7 cut(s) 10, 250, 302, 363, 923, 1018, 1096
Hpy8I GTNNAC 5 cut(s) 88, 389, 793, 968, 995
HpyAV CCTTC 3 cut(s) 343, 384, 929
HpyCH4III ACNGT 2 cut(s) 92, 965
HpyCH4IV ACGT 1 cut(s) 185
HpyCH4V TGCA 3 cut(s) 101, 282, 1067
HpyF10VI GCNNNNNNNGC 3 cut(s) 907, 1140, 1164
HpyF3I CTNAG 1 cut(s) 797
HpySE526I ACGT 1 cut(s) 185
Hsp92II CATG 6 cut(s) 24, 78, 133, 565, 979, 1139
Kzo9I GATC 6 cut(s) 6, 58, 304, 412, 881, 1012
LmnI GCTCC 2 cut(s) 832, 1003
Lsp1109I GCAGC 1 cut(s) 294
LweI GCATC 4 cut(s) 222, 269, 1054, 1089
MaeI CTAG 2 cut(s) 39, 272
MaeII ACGT 1 cut(s) 185
MaeIII GTNAC 3 cut(s) 106, 800, 1079
MalI GATC 6 cut(s) 8, 60, 306, 414, 883, 1014
MboI GATC 6 cut(s) 6, 58, 304, 412, 881, 1012
MboII GAAGA 3 cut(s) 1061, 1064, 1109
MflI RGATCY 1 cut(s) 1012
MhlI GDGCHC 2 cut(s) 138, 1160
MlsI TGGCCA 1 cut(s) 731
MluCI AATT 7 cut(s) 113, 346, 724, 775, 784, 875, 926
MluNI TGGCCA 1 cut(s) 731
MlyI GAGTC 1 cut(s) 93
MnlI CCTC 6 cut(s) 450, 464, 466, 491, 697, 791
Mox20I TGGCCA 1 cut(s) 731
Mph1103I ATGCAT 1 cut(s) 1069
MroXI GAANNNNTTC 2 cut(s) 775, 911
MscI TGGCCA 1 cut(s) 731
MseI TTAA 5 cut(s) 257, 488, 657, 960, 1070
MslI CAYNNNNRTG 1 cut(s) 54
Msp20I TGGCCA 1 cut(s) 731
MspA1I CMGCKG 1 cut(s) 430
MspI CCGG 3 cut(s) 156, 603, 675
MspR9I CCNGG 3 cut(s) 210, 468, 604
MvaI CCWGG 2 cut(s) 210, 468
MwoI GCNNNNNNNGC 3 cut(s) 907, 1140, 1164
NciI CCSGG 1 cut(s) 604
NcoI CCATGG 2 cut(s) 74, 129
NdeII GATC 6 cut(s) 6, 58, 304, 412, 881, 1012
NlaIII CATG 6 cut(s) 24, 78, 133, 565, 979, 1139
NlaIV GGNNCC 2 cut(s) 532, 1159
NmuCI GTSAC 2 cut(s) 106, 1079
NsiI ATGCAT 1 cut(s) 1069
PceI AGGCCT 1 cut(s) 750
PdmI GAANNNNTTC 2 cut(s) 775, 911
PfeI GAWTC 2 cut(s) 17, 571
PfoI TCCNGGA 1 cut(s) 466
PkrI GCNGC 1 cut(s) 284
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
PpuMI RGGWCCY 1 cut(s) 893
PshBI ATTAAT 1 cut(s) 1070
Psp5II RGGWCCY 1 cut(s) 893
Psp6I CCWGG 2 cut(s) 208, 466
PspFI CCCAGC 1 cut(s) 42
PspGI CCWGG 2 cut(s) 208, 466
PspN4I GGNNCC 2 cut(s) 532, 1159
PspPI GGNCC 3 cut(s) 437, 893, 1146
PspPPI RGGWCCY 1 cut(s) 893
PsuI RGATCY 1 cut(s) 1012
PvuII CAGCTG 1 cut(s) 430
RsaI GTAC 2 cut(s) 94, 967
RsaNI GTAC 2 cut(s) 93, 966
RseI CAYNNNNRTG 1 cut(s) 54
SaqAI TTAA 5 cut(s) 257, 488, 657, 960, 1070
SatI GCNGC 1 cut(s) 283
Sau3AI GATC 6 cut(s) 6, 58, 304, 412, 881, 1012
Sau96I GGNCC 3 cut(s) 437, 893, 1146
SchI GAGTC 1 cut(s) 93
ScrFI CCNGG 3 cut(s) 210, 468, 604
SduI GDGCHC 2 cut(s) 138, 1160
SfaNI GCATC 4 cut(s) 222, 269, 1054, 1089
SfcI CTRYAG 2 cut(s) 1044, 1089
SinI GGWCC 2 cut(s) 437, 893
SmiMI CAYNNNNRTG 1 cut(s) 54
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
Sse9I AATT 7 cut(s) 113, 346, 724, 775, 784, 875, 926
SseBI AGGCCT 1 cut(s) 750
SspMI CTAG 2 cut(s) 39, 272
StuI AGGCCT 1 cut(s) 750
StyD4I CCNGG 3 cut(s) 208, 466, 602
StyI CCWWGG 2 cut(s) 74, 129
TaaI ACNGT 2 cut(s) 92, 965
TaiI ACGT 1 cut(s) 188
TaqI TCGA 1 cut(s) 174
TasI AATT 7 cut(s) 113, 346, 724, 775, 784, 875, 926
TatI WGTACW 1 cut(s) 92
TfiI GAWTC 2 cut(s) 17, 571
Tru1I TTAA 5 cut(s) 257, 488, 657, 960, 1070
Tru9I TTAA 5 cut(s) 257, 488, 657, 960, 1070
TscAI CASTG 4 cut(s) 295, 975, 1136, 1184
TseFI GTSAC 2 cut(s) 106, 1079
TseI GCWGC 1 cut(s) 282
Tsp45I GTSAC 2 cut(s) 106, 1079
TspDTI ATGAA 5 cut(s) 9, 192, 563, 732, 788
TspRI CASTG 4 cut(s) 295, 975, 1136, 1184
VpaK11BI GGWCC 2 cut(s) 437, 893
VspI ATTAAT 1 cut(s) 1070
XapI RAATTY 4 cut(s) 346, 724, 775, 875
XcmI CCANNNNNNNNNTGG 2 cut(s) 868, 1168
XmiI GTMKAC 2 cut(s) 792, 994
XmnI GAANNNNTTC 2 cut(s) 775, 911
XspI CTAG 2 cut(s) 39, 272
Zsp2I ATGCAT 1 cut(s) 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.