FvH4_6g15300

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002940
Physical Location & Seq
Forward (+)
9529850 .. 9531888
2039 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g15300.t1

Sequence Viewer

Length: 1491 bp
ATGGAGGGACCTGAATATGGTGAAGGGTTGGTTTTTGAAGCCAAGGATAGTTGTAGGAATGTTGAAAGTTTGTTAATTTCTGATAAAGATGAGACTTCGAATGAACTTATGAGTAAAAACGATTGTCAAGAGTTGAATGCTAAGAGGGTGACTTGTGATGGAGTCGAAGATGAGGGGTTGGAGGTTGATGTGGGAGGAGAGGGCAACACTGATTCGCTCAGTGTTGCTGAATTTATAAGGAATTCGAAAATGGATGGGAATGTGGGGAGTGGTGTTGATGGAAAGTCTTGTTCTTCGAAGATGGAATTTGGAGTTTCTGACATGGTATGGGGGAAAGTGAGGACTCATCCCTGGTGGCCCGGGCAGATTTGTGATCCTTCGGCTGCGTCAGAACTGGCTAAGAAGTGTTTTAAGAAGGGTAGTTATCTGGTAGCTTACTTTGGGGACCAGACATTTGCTTGGAATGAAGCAGCAAAGATTAAGCCCTTTCTGGAGCACTGCTCAAAAATGGAGAAGCAGAGTGATAAGGAAGAGTTCCGAAACGCTGTTGATTGTGCTTTTGAGGAAGTGTCAAGAAGGGTTGAGTTTGGGCTGGCCTGTTCTTGCATATCCAAAGGAGAGTATGAAAAACTCAAGACGCAGAAGATTATTAATGCTGGAATCAGAAAAGAAGCAAGCGAAAGAGATGGTGGTGACAGTGCTCTTAGCGCCGCTTTTTTTGAACCTGCGAAAGTCATCAAGTTTATAAGAAAGTATGCACAGTTGCCATACGGCAAAGCTGACAGACTAGAACTTGTAACATCCCATGCCCAGCTATCAGCTTTCAGGCGCTGGAAGAGTTATGTGCCTCTGCCAGAGGAGAATGATACTGATATTTCCTTTTTGGGAGAGAAGAACCGCTGCAATGAAGCAAGTGATGAAGCGCTCTCTGTAATTGAGAATGAGAAGTTGGTGCAAAAACAAGAGAGAGAAGATACATCATTGCATGAATGCATGGACATATCAGATTCAACAGCTCAGAAGTGTTTGTTTCCAATGAGTGAATCAGAAGGGAAAGCTGGCAGTGAATTGATTTTGCAACCTTGCAACAAAAGAAAGGATCTTGATGCTATAGCTGATGAAGATGTACTCCCAGTAATTAAGGATGGCAAGTTGGTGGGGATATTAGAAACTAAAGATAACTCATTGCATAAGTGTTGGCACCTCCCTGGAGATAGTATAAATCCGAGTGAAGAACAAATCTTGTCAGATTTGATGGGTACGGATTGTTTGTCCAACTTATTGAGCGAGAATGGATTAAAAGAAGGGAAATGTGATGGCAGATTGATTTCACAACCTTCTACTAAGAAACGGAAGGGAGTCGAAGCTATAGCTGATGACGGTGCTGTGGAACATGTGAAGAGTGATTCATCGCAGGGGATCGGTAGCAATTTGAACAATCAGCCATTTGGACTTGAAGAGTTTTTTAACCTGTTTTTGCACCAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

497

Amino Acids

54.85

Weight (kDa)

4.86

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 107 - 189 1.2e-19 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 236, 746
Acc36I ACCTGC 1 cut(s) 733
AccB1I GGYRCC 1 cut(s) 1200
AciI CCGC 2 cut(s) 711, 898
AclWI GGATC 3 cut(s) 368, 1107, 1427
AcsI RAATTY 3 cut(s) 230, 241, 305
AfaI GTAC 2 cut(s) 1128, 1261
AfeI AGCGCT 1 cut(s) 924
AfiI CCNNNNNNNGG 1 cut(s) 17
AflIII ACRYGT 1 cut(s) 1393
AgsI TTSAA 7 cut(s) 38, 65, 136, 722, 1011, 1435, 1457
AjnI CCWGG 2 cut(s) 350, 1207
AjuI GAANNNNNNNTTGG 2 cut(s) 932, 964
AluBI AGCT 9 cut(s) 434, 779, 814, 821, 1016, 1058, 1115, 1367, 1373
AluI AGCT 9 cut(s) 434, 779, 814, 821, 1016, 1058, 1115, 1367, 1373
Alw21I GWGCWC 2 cut(s) 498, 703
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 3 cut(s) 368, 1107, 1427
AlwNI CAGNNNCTG 1 cut(s) 831
Ama87I CYCGRG 1 cut(s) 359
Aor51HI AGCGCT 1 cut(s) 924
AoxI GGCC 2 cut(s) 356, 594
ApeKI GCWGC 3 cut(s) 383, 470, 900
ApoI RAATTY 3 cut(s) 230, 241, 305
ArsI GACNNNNNNTTYG 2 cut(s) 437, 469
AseI ATTAAT 1 cut(s) 651
AspLEI GCGC 3 cut(s) 710, 831, 925
AspS9I GGNCC 3 cut(s) 8, 357, 445
AsuC2I CCSGG 2 cut(s) 360, 361
AsuHPI GGTGA 3 cut(s) 32, 160, 704
AsuII TTCGAA 3 cut(s) 98, 245, 296
AvaI CYCGRG 1 cut(s) 359
AvaII GGWCC 2 cut(s) 8, 445
BanI GGYRCC 1 cut(s) 1200
Bbv12I GWGCWC 2 cut(s) 498, 703
BbvI GCAGC 3 cut(s) 370, 482, 887
BccI CCATC 8 cut(s) 152, 248, 272, 295, 680, 1139, 1249, 1310
BceAI ACGGC 1 cut(s) 787
BciT130I CCWGG 2 cut(s) 352, 1209
BcnI CCSGG 2 cut(s) 360, 361
BcoDI GTCTC 1 cut(s) 86
BfaI CTAG 1 cut(s) 788
BfmI CTRYAG 2 cut(s) 1110, 1368
BfoI RGCGCY 3 cut(s) 711, 832, 926
BfuAI ACCTGC 1 cut(s) 733
BisI GCNGC 4 cut(s) 384, 471, 711, 901
BlsI GCNGC 4 cut(s) 385, 472, 712, 902
Bme1390I CCNGG 4 cut(s) 352, 360, 361, 1209
Bme18I GGWCC 2 cut(s) 8, 445
BmeT110I CYCGRG 1 cut(s) 359
BmgT120I GGNCC 3 cut(s) 8, 357, 445
BmiI GGNNCC 3 cut(s) 9, 446, 1202
BmrFI CCNGG 4 cut(s) 352, 360, 361, 1209
BmrI ACTGGG 1 cut(s) 1127
BmsI GCATC 1 cut(s) 1096
BmuI ACTGGG 1 cut(s) 1127
BplI GAGNNNNNCTC 2 cut(s) 485, 517
BpmI CTGGAG 2 cut(s) 512, 1230
Bpu14I TTCGAA 3 cut(s) 98, 245, 296
BpuEI CTTGAG 1 cut(s) 617
BpuMI CCSGG 2 cut(s) 360, 361
BsaJI CCNNGG 4 cut(s) 42, 350, 359, 1207
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse1I ACTGG 3 cut(s) 399, 1133, 1483
Bse3DI GCAATG 3 cut(s) 910, 980, 1184
BseBI CCWGG 2 cut(s) 352, 1209
BseDI CCNNGG 4 cut(s) 42, 350, 359, 1207
BseGI GGATG 4 cut(s) 259, 346, 800, 1150
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMI GCAATG 3 cut(s) 910, 980, 1184
BseMII CTCAG 2 cut(s) 232, 1031
BseNI ACTGG 3 cut(s) 399, 1133, 1483
BseRI GAGGAG 2 cut(s) 210, 872
BseXI GCAGC 3 cut(s) 370, 482, 887
BseYI CCCAGC 1 cut(s) 810
BshFI GGCC 2 cut(s) 358, 596
BshNI GGYRCC 1 cut(s) 1200
BsiHKAI GWGCWC 2 cut(s) 498, 703
BsiHKCI CYCGRG 1 cut(s) 359
BsiSI CCGG 1 cut(s) 360
BslFI GGGAC 2 cut(s) 21, 458
BslI CCNNNNNNNGG 1 cut(s) 17
BsmAI GTCTC 1 cut(s) 86
BsmFI GGGAC 2 cut(s) 21, 458
BsmI GAATGC 2 cut(s) 142, 995
BsnI GGCC 2 cut(s) 358, 596
BsoBI CYCGRG 1 cut(s) 359
Bsp119I TTCGAA 3 cut(s) 98, 245, 296
Bsp1286I GDGCHC 2 cut(s) 498, 703
Bsp143I GATC 3 cut(s) 373, 1099, 1419
BspACI CCGC 2 cut(s) 711, 898
BspANI GGCC 2 cut(s) 358, 596
BspCNI CTCAG 2 cut(s) 231, 1030
BspLI GGNNCC 3 cut(s) 9, 446, 1202
BspMI ACCTGC 1 cut(s) 733
BspPI GGATC 3 cut(s) 368, 1107, 1427
BspT104I TTCGAA 3 cut(s) 98, 245, 296
BspT107I GGYRCC 1 cut(s) 1200
BsrDI GCAATG 3 cut(s) 910, 980, 1184
BsrI ACTGG 3 cut(s) 399, 1133, 1483
BssECI CCNNGG 4 cut(s) 42, 350, 359, 1207
BssMI GATC 3 cut(s) 373, 1099, 1419
BssT1I CCWWGG 1 cut(s) 42
Bst2UI CCWGG 2 cut(s) 352, 1209
Bst4CI ACNGT 3 cut(s) 698, 762, 1382
Bst6I CTCTTC 4 cut(s) 525, 830, 1394, 1452
BstBI TTCGAA 3 cut(s) 98, 245, 296
BstC8I GCNNGC 3 cut(s) 594, 676, 1060
BstDEI CTNAG 6 cut(s) 141, 218, 399, 704, 1017, 1344
BstF5I GGATG 4 cut(s) 259, 346, 800, 1150
BstH2I RGCGCY 3 cut(s) 711, 832, 926
BstHHI GCGC 3 cut(s) 710, 831, 925
BstKTI GATC 3 cut(s) 376, 1102, 1422
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 3 cut(s) 373, 1099, 1419
BstMWI GCNNNNNNNGC 1 cut(s) 707
BstNI CCWGG 2 cut(s) 352, 1209
BstNSI RCATGY 1 cut(s) 1397
BstSCI CCNGG 4 cut(s) 350, 358, 359, 1207
BstSFI CTRYAG 2 cut(s) 1110, 1368
BstV1I GCAGC 3 cut(s) 370, 482, 887
BstX2I RGATCY 1 cut(s) 1099
BstYI RGATCY 1 cut(s) 1099
BsuRI GGCC 2 cut(s) 358, 596
BtgZI GCGATG 1 cut(s) 1395
BtsCI GGATG 4 cut(s) 259, 346, 800, 1150
BtsI GCAGTG 2 cut(s) 496, 1069
BtsIMutI CAGTG 5 cut(s) 207, 226, 496, 703, 1069
BveI ACCTGC 1 cut(s) 733
Cac8I GCNNGC 3 cut(s) 594, 676, 1060
CaiI CAGNNNCTG 1 cut(s) 831
CfoI GCGC 3 cut(s) 710, 831, 925
Cfr13I GGNCC 3 cut(s) 8, 357, 445
Cfr9I CCCGGG 1 cut(s) 359
CseI GACGC 2 cut(s) 375, 646
Csp6I GTAC 2 cut(s) 1127, 1260
CviAII CATG 5 cut(s) 322, 806, 986, 994, 1394
CviQI GTAC 2 cut(s) 1127, 1260
DdeI CTNAG 6 cut(s) 141, 218, 399, 704, 1017, 1344
DpnI GATC 3 cut(s) 375, 1101, 1421
DpnII GATC 3 cut(s) 373, 1099, 1419
Eam1104I CTCTTC 4 cut(s) 525, 830, 1394, 1452
EarI CTCTTC 4 cut(s) 525, 830, 1394, 1452
Eco130I CCWWGG 1 cut(s) 42
Eco47I GGWCC 2 cut(s) 8, 445
Eco47III AGCGCT 1 cut(s) 924
Eco88I CYCGRG 1 cut(s) 359
EcoO109I RGGNCCY 1 cut(s) 8
EcoRI GAATTC 1 cut(s) 241
EcoRII CCWGG 2 cut(s) 350, 1207
EcoT14I CCWWGG 1 cut(s) 42
EcoT22I ATGCAT 1 cut(s) 995
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 5 cut(s) 325, 809, 989, 997, 1397
FaqI GGGAC 2 cut(s) 21, 458
FatI CATG 5 cut(s) 321, 805, 985, 993, 1393
Fnu4HI GCNGC 4 cut(s) 384, 471, 711, 901
FokI GGATG 4 cut(s) 266, 333, 787, 1157
Fsp4HI GCNGC 4 cut(s) 384, 471, 711, 901
FspBI CTAG 1 cut(s) 788
GlaI GCGC 3 cut(s) 709, 830, 924
GluI GCNGC 4 cut(s) 384, 471, 711, 901
GsaI CCCAGC 1 cut(s) 814
GsuI CTGGAG 2 cut(s) 512, 1230
HaeII RGCGCY 3 cut(s) 711, 832, 926
HaeIII GGCC 2 cut(s) 358, 596
HapII CCGG 1 cut(s) 360
HgaI GACGC 2 cut(s) 375, 646
HhaI GCGC 3 cut(s) 710, 831, 925
Hin1II CATG 5 cut(s) 325, 809, 989, 997, 1397
Hin6I GCGC 3 cut(s) 708, 829, 923
HinP1I GCGC 3 cut(s) 708, 829, 923
HinfI GANTC 8 cut(s) 162, 212, 343, 660, 1007, 1043, 1359, 1406
HpaII CCGG 1 cut(s) 360
HphI GGTGA 3 cut(s) 32, 160, 704
Hpy188III TCNNGA 5 cut(s) 128, 491, 573, 634, 1103
HpyAV CCTTC 8 cut(s) 17, 387, 409, 570, 1043, 1298, 1347, 1348
HpyCH4III ACNGT 3 cut(s) 698, 762, 1382
HpyF10VI GCNNNNNNNGC 1 cut(s) 707
HpyF3I CTNAG 6 cut(s) 141, 218, 399, 704, 1017, 1344
Hsp92II CATG 5 cut(s) 325, 809, 989, 997, 1397
HspAI GCGC 3 cut(s) 708, 829, 923
Kzo9I GATC 3 cut(s) 373, 1099, 1419
LmnI GCTCC 1 cut(s) 493
Lsp1109I GCAGC 3 cut(s) 370, 482, 887
LweI GCATC 1 cut(s) 1096
MaeI CTAG 1 cut(s) 788
MaeIII GTNAC 3 cut(s) 148, 692, 796
MalI GATC 3 cut(s) 375, 1101, 1421
MboI GATC 3 cut(s) 373, 1099, 1419
MflI RGATCY 1 cut(s) 1099
MhlI GDGCHC 2 cut(s) 498, 703
MluCI AATT 8 cut(s) 75, 230, 241, 305, 933, 1067, 1137, 1429
MlyI GAGTC 3 cut(s) 171, 337, 1368
MmeI TCCRAC 2 cut(s) 159, 1299
Mph1103I ATGCAT 1 cut(s) 995
MseI TTAA 8 cut(s) 74, 411, 480, 651, 1140, 1298, 1467, 1489
MspA1I CMGCKG 1 cut(s) 900
MspI CCGG 1 cut(s) 360
MspR9I CCNGG 4 cut(s) 352, 360, 361, 1209
Mva1269I GAATGC 2 cut(s) 142, 995
MvaI CCWGG 2 cut(s) 352, 1209
MwoI GCNNNNNNNGC 1 cut(s) 707
NciI CCSGG 2 cut(s) 360, 361
NdeII GATC 3 cut(s) 373, 1099, 1419
NlaIII CATG 5 cut(s) 325, 809, 989, 997, 1397
NlaIV GGNNCC 3 cut(s) 9, 446, 1202
NmuCI GTSAC 2 cut(s) 148, 692
NsiI ATGCAT 1 cut(s) 995
NspI RCATGY 1 cut(s) 1397
NspV TTCGAA 3 cut(s) 98, 245, 296
PciI ACATGT 1 cut(s) 1393
PctI GAATGC 2 cut(s) 142, 995
PfeI GAWTC 5 cut(s) 212, 660, 1007, 1043, 1406
PkrI GCNGC 4 cut(s) 385, 472, 712, 902
PleI GAGTC 3 cut(s) 170, 337, 1367
PpsI GAGTC 3 cut(s) 170, 337, 1367
PpuMI RGGWCCY 1 cut(s) 8
PscI ACATGT 1 cut(s) 1393
PshBI ATTAAT 1 cut(s) 651
PsiI TTATAA 2 cut(s) 236, 746
Psp5II RGGWCCY 1 cut(s) 8
Psp6I CCWGG 2 cut(s) 350, 1207
PspFI CCCAGC 1 cut(s) 810
PspGI CCWGG 2 cut(s) 350, 1207
PspN4I GGNNCC 3 cut(s) 9, 446, 1202
PspPI GGNCC 3 cut(s) 8, 357, 445
PspPPI RGGWCCY 1 cut(s) 8
PstNI CAGNNNCTG 1 cut(s) 831
PsuI RGATCY 1 cut(s) 1099
RsaI GTAC 2 cut(s) 1128, 1261
RsaNI GTAC 2 cut(s) 1127, 1260
SaqAI TTAA 8 cut(s) 74, 411, 480, 651, 1140, 1298, 1467, 1489
SatI GCNGC 4 cut(s) 384, 471, 711, 901
Sau3AI GATC 3 cut(s) 373, 1099, 1419
Sau96I GGNCC 3 cut(s) 8, 357, 445
SchI GAGTC 3 cut(s) 171, 337, 1368
ScrFI CCNGG 4 cut(s) 352, 360, 361, 1209
SduI GDGCHC 2 cut(s) 498, 703
SfaNI GCATC 1 cut(s) 1096
SfcI CTRYAG 2 cut(s) 1110, 1368
SfuI TTCGAA 3 cut(s) 98, 245, 296
SinI GGWCC 2 cut(s) 8, 445
SmaI CCCGGG 1 cut(s) 361
SmlI CTYRAG 1 cut(s) 632
SmoI CTYRAG 1 cut(s) 632
SrfI GCCCGGGC 1 cut(s) 361
Sse9I AATT 8 cut(s) 75, 230, 241, 305, 933, 1067, 1137, 1429
SsiI CCGC 2 cut(s) 711, 898
SspMI CTAG 1 cut(s) 788
StyD4I CCNGG 4 cut(s) 350, 358, 359, 1207
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 3 cut(s) 698, 762, 1382
TaqI TCGA 5 cut(s) 98, 165, 245, 296, 1362
TasI AATT 8 cut(s) 75, 230, 241, 305, 933, 1067, 1137, 1429
TatI WGTACW 1 cut(s) 1126
TauI GCSGC 1 cut(s) 713
TfiI GAWTC 5 cut(s) 212, 660, 1007, 1043, 1406
Tru1I TTAA 8 cut(s) 74, 411, 480, 651, 1140, 1298, 1467, 1489
Tru9I TTAA 8 cut(s) 74, 411, 480, 651, 1140, 1298, 1467, 1489
TscAI CASTG 5 cut(s) 214, 226, 503, 703, 1069
TseFI GTSAC 2 cut(s) 148, 692
TseI GCWGC 3 cut(s) 383, 470, 900
Tsp45I GTSAC 2 cut(s) 148, 692
TspDTI ATGAA 8 cut(s) 117, 480, 639, 921, 933, 1002, 1134, 1398
TspGWI ACGGA 2 cut(s) 1277, 1366
TspMI CCCGGG 1 cut(s) 359
TspRI CASTG 5 cut(s) 214, 226, 503, 703, 1069
VpaK11BI GGWCC 2 cut(s) 8, 445
VspI ATTAAT 1 cut(s) 651
XapI RAATTY 3 cut(s) 230, 241, 305
XceI RCATGY 1 cut(s) 1397
XmaI CCCGGG 1 cut(s) 359
XspI CTAG 1 cut(s) 788
Zsp2I ATGCAT 1 cut(s) 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.