RchiOBHm_Chr3g0468791

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002940
Physical Location & Seq
Forward (+)
14694606 .. 14698652
4047 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43466

Sequence Viewer

Length: 3243 bp
ATGGCTCGGATAAGAAAGCACGGGGCTAATTTCTCTGGGGAGGGAGGAGTTGAGAGCGTTGATGATAGTGATGCAGTAGTGGGTGAGGACATGAGTGAGGGTTTGGAAGAGGGAGATGAATTGGTAGATGGAGGTGGTGATTCTGGAGGGAAAGTAGGACCTGTAGATATGGAGATTGATGAGAAAGGTGGAGATGGAGTGACAGAGGGTTTGGAGGGGACTCAATTTGGGGGTACTGATGCGAATGCAGCGAGTGAGAAAGGGTCGGTTTCGGAATCGAAGGATGGTTCTAAGGGAGTTGAAAGTTCAGTCCTTAGTGACAAGGATAGGGGTAAAGATGAGATTGTAAGGGAATGCGTGGAACCTGTAGCTGTGGGTACTGTGGAAGGGAACCTCTCGATTAGTTTTGAGGCGAAGGAGGATGGAACAGTTGAGGGAAAGGATGAATTGATGAGAGATGACAAAGAAGAGGAAGTTGCACAAGTAGATGAGGAAGAGAGAGAAAGAGAACAGAATGTGGAGTGTAGCCAGAGTAGCCATGCTACAAGTTCTAACGTGGAGCATGAGGCTACAGGGTCTGTTGGAGAAATACCGCAGGACATCCCAGCTGTGGAAGTGAAAATTGCCGGTATCGATGAGTCTGGGCATGATGGAGACCTGAATGCAAGCACTGGTTCCCAGATGGTTATGTCTGGTGGACTAGAAAATCAGACGTTGGAAGTTAATGTGGGAGTAAAGACCGGTGAAGATGTCTTGATGCCTGAATATGTTTCCCCAGCAGAGGTTTCTGTGAAATCTGAAAATGGGCATAATTTGATTGACATGGCTATTGATTTGGAACCGTATGCTGGAAAGGATGGGAATGTTTCAGATGATAGTAATGTTTCTGGTCCCTCACAACCAGAATTTCAAGCTTTTGATCTAGTATGGGGCAAAATTAGGAGCCATCCATGGTGGCCTGGGCAGATATGTGATCCTTCAGCTTCATCAAAACTTGCAAAGAAGTATTTTAAGAAGCACAGTTATCTGATAGCTTATTTTTGGGATTGTACATTTGCTTGGAATGAAGCACCACAAATTAAGCCCTTTAGGGAGAACTTTTCACAAATGGAAAAGCAGAGCAATATGGAGGAATTCCAAAATGCAGTTGCTTGTGCTCTGGATGAAGTCTCAAGGCGAGTAGAGTTTGGCTTGGCCTGTTCTTGCATTTCCAAAGAAGTTTATGCAAAGCTCAAAACACAGACGATCATTAATGCTGGAATCAAGGAAGAAGCATGTAGAAGAGATGGTGGTGATAGTTCACTGGGTGCTTCTTCTTTTGAACCCTTGAAACTCATTAACTTTGCAAAACAAGCCGCCAAGCTGCCATTTGGCAGAGCTGACAGACTCGAACTTGTTACATCACGGGCCCAATTATCATCTTTCTATCGTTGGAAGGGTTATTCTCAGCTGCCAGAGTTCAATTTTCTTGGTGGGTTGCTGGAAAATGATGCCGACATTTCACTATTGAGAGAAAAGGAGCAATTTAATGAACTACGGGAAGTTGCACTTCCAGTAATTAAGGATGAGGAATTGGAGCAGAAATCAAAAGGGAAAAGCTCCTCATTGCATAAGCGTAAGCACATATCTGGAGATAGTTCCCAACCAGGTAAAAAAGGGAAAAACTCCTCAAATGTAACTGCTGAGAAGGGTTTGTCTACTCCCATGAGTGAGAATGGATCAGAAAGGAAAGCTGACTGTAAGTTGGTTTCACAATCCTCAAGTAAGAAACGAAAGGCAGTTGATGGAGCTATGCCTGATGACTCTGCGGTGAAACATAGTAAAAGCACTTCATCAGCAGTGGAAGATACTGTTCCTTTACAGAATAAGCAGACTTTTAGAGTTGGAGATAGAATCTGTAGGGTTGCAACCCTACTAAATGGGTCAAGTCCAATATTCAGGCACAATGCAGCACCTGCAGATGCTGAAGTTCAGGATGAAGGCAAAGAGAAATCTTCATTGCAGAAACCACAAACTGAAAAGCTGATTGACATGGAGAATCCTTCTCCTGATGAGATGCTGTCTCAGCTTTGCTTGGCTGCCATGAATCCCAATAAAGGGTACAGCATGTTGATTTCCTTGATGGGTTGCTTCTCAAAATTTAGGAATACTATATGCCTGGAACCTCCCAGTCCAGATGAGGATCAATTGCCTTTGGGACAAATATTTGGTAGCAAGCGCAGTAAGAAATCAACCAAGACTGGAAAGAAATCAATCAAACCACGCATCTCTGAAAAATCTGAGTTGGAGCTGGTCAAAGACTCTTATTCTTCTGATAAGATAGTTAAGAGTCAAAATGAAAATGGAGAGTTATTGCTTGGAGCTGCAAGCGATAAAGATACATGTGTTGTGGAGTCACAGTCAAGTCCTGATTCGGAAGCACTGAAGCCAGTTCTTGACAATAAAAATGGAGAGTTGGTGTCTGCTACAAGCGATAAGGATACTTATACTGTTGAACTGCAAGCAACTCTCGAGTTAAATCCCAATTTGGATTCTGAACAGAACATTGTTTATGGAGATATTGATTCAAAAGCAGTGAACCCAGCTCTGGATGATGAAAATGGAGCATTAGTACGTGAGCCCATCTCTGTTGTTGAGTCACCAGCAGATCCCAAGTTAAATCCCAATTTGGATTTTGAACAGGAAAATACAGTAAGAGATACTGGTGCGGAAGCAGTGAAGCCAGCTCTTGACGATGAAAGTGGAGAGTTGGTGGCTGGAGCTCTAAGTAATAAGGATGCTTCCGCTGTGGATTCGCAAGCAAGTCTTGAGTTCAATCCCAATATGGATTCTGCACTGAAATTTCCTGATGGAGATTATGATTCAGAAGCAGTGAAGCCAGCTTCCCCAAGTGAGAGTTGTGAGGAAGATTCTCCCGCAGCTCTGATCTTGAACTTTTCAGACTTGGAGTCTCTTCCCTCGGAAGCAAGTCTAAACAATATTTTTAGCATCTTCGGGCCTCTGAATGAATCGCAAACCGAATTGTTGAAGAAGACAAACCGGGCCAAAGTGGTTTTCAGGCGGCGTTCTGATGCTCAAAAAGCTTTCAGCAGTGCCGGGAAATACAGTACCTTTGGACCTTCACTTGAAAGTTATCGCCTCAAGTATCCGCTATCACCAAGTAAAGCTTCTCCTTCTCCGAAGGTGGCAAAGCAAGGAAAAATGGATGCATCTGCAGAAGGTGATGGAGCTAGAAAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1080

Amino Acids

116.64

Weight (kDa)

4.71

Isoelectric Point (pI)

48.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 307 - 389 2e-17 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1963
Acc36I ACCTGC 1 cut(s) 1963
AccI GTMKAC 1 cut(s) 1697
AciI CCGC 8 cut(s) 593, 1356, 1808, 2708, 2784, 2916, 3061, 3149
AclWI GGATC 4 cut(s) 968, 1726, 2190, 2642
AcsI RAATTY 4 cut(s) 905, 1133, 2138, 2840
AcuI CTGAAG 3 cut(s) 963, 1986, 2444
AdeI CACNNNGTG 1 cut(s) 1307
AfaI GTAC 6 cut(s) 235, 379, 1051, 2102, 2613, 3109
AfiI CCNNNNNNNGG 6 cut(s) 610, 781, 848, 2096, 2097, 2587
AflIII ACRYGT 1 cut(s) 2381
AgeI ACCGGT 1 cut(s) 740
AhdI GACNNNNNGTC 1 cut(s) 2947
AjnI CCWGG 3 cut(s) 958, 1645, 2157
AjuI GAANNNNNNNTTGG 4 cut(s) 3038, 3070, 3151, 3183
Alw21I GWGCWC 2 cut(s) 1159, 2764
Alw26I GTCTC 4 cut(s) 648, 1174, 2067, 2955
AlwI GGATC 4 cut(s) 968, 1726, 2190, 2642
AlwNI CAGNNNCTG 3 cut(s) 578, 1955, 1964
Ama87I CYCGRG 1 cut(s) 2510
AoxI GGCC 5 cut(s) 956, 1194, 1407, 2996, 3042
ApaI GGGCCC 1 cut(s) 1411
ApeKI GCWGC 7 cut(s) 248, 1363, 1450, 1949, 2078, 2363, 2918
ApoI RAATTY 4 cut(s) 905, 1133, 2138, 2840
ArsI GACNNNNNNTTYG 2 cut(s) 193, 225
AseI ATTAAT 1 cut(s) 1251
AsiGI ACCGGT 1 cut(s) 740
AspLEI GCGC 1 cut(s) 2220
AspS9I GGNCC 7 cut(s) 158, 890, 1407, 1408, 2996, 3042, 3116
AsuC2I CCSGG 2 cut(s) 3041, 3097
AsuHPI GGTGA 8 cut(s) 95, 149, 755, 1304, 1822, 2631, 3147, 3233
AvaI CYCGRG 1 cut(s) 2510
AvaII GGWCC 3 cut(s) 158, 890, 3116
BaeGI GKGCMC 1 cut(s) 1411
BaeI ACNNNNGTAYC 2 cut(s) 2370, 2403
BanII GRGCYC 3 cut(s) 1411, 2622, 2764
BarI GAAGNNNNNNTAC 2 cut(s) 1813, 1845
BbsI GAAGAC 1 cut(s) 3038
Bbv12I GWGCWC 2 cut(s) 1159, 2764
BbvI GCAGC 7 cut(s) 260, 1350, 1437, 1961, 2065, 2350, 2930
BciT130I CCWGG 3 cut(s) 960, 1647, 2159
BciVI GTATCC 2 cut(s) 2473, 3156
BcnI CCSGG 2 cut(s) 3041, 3097
BcoDI GTCTC 4 cut(s) 648, 1174, 2067, 2955
BfaI CTAG 3 cut(s) 701, 923, 3231
BfmI CTRYAG 6 cut(s) 162, 366, 570, 1897, 1956, 3213
BfuAI ACCTGC 1 cut(s) 1963
BfuI GTATCC 2 cut(s) 2473, 3156
BisI GCNGC 9 cut(s) 249, 1356, 1364, 1451, 1950, 2079, 2364, 2919, 3062
BlsI GCNGC 9 cut(s) 250, 1357, 1365, 1452, 1951, 2080, 2365, 2920, 3063
Bme1390I CCNGG 5 cut(s) 960, 1647, 2159, 3041, 3097
Bme18I GGWCC 3 cut(s) 158, 890, 3116
BmeRI GACNNNNNGTC 1 cut(s) 2947
BmeT110I CYCGRG 1 cut(s) 2510
BmgT120I GGNCC 7 cut(s) 158, 890, 1407, 1408, 2996, 3042, 3116
BmiI GGNNCC 8 cut(s) 363, 392, 676, 840, 892, 944, 1409, 2163
BmrFI CCNGG 5 cut(s) 960, 1647, 2159, 3041, 3097
BmrI ACTGGG 2 cut(s) 1313, 2163
BmuI ACTGGG 2 cut(s) 1313, 2163
BpiI GAAGAC 1 cut(s) 3038
BplI GAGNNNNNCTC 2 cut(s) 2609, 2641
BpmI CTGGAG 3 cut(s) 165, 1650, 2778
BpuEI CTTGAG 4 cut(s) 1156, 1744, 2828, 3125
BpuMI CCSGG 2 cut(s) 3041, 3097
Bsa29I ATCGAT 1 cut(s) 633
BsaAI YACGTR 1 cut(s) 2615
BsaBI GATNNNNATC 1 cut(s) 2181
BsaI GGTCTC 1 cut(s) 648
BsaJI CCNNGG 3 cut(s) 950, 959, 2958
BsaWI WCCGGW 1 cut(s) 740
Bsc4I CCNNNNNNNGG 6 cut(s) 610, 781, 848, 2096, 2097, 2587
Bse118I RCCGGY 2 cut(s) 626, 740
Bse1I ACTGG 7 cut(s) 676, 1308, 1553, 2169, 2245, 2429, 2707
Bse3DI GCAATG 2 cut(s) 1604, 1997
Bse8I GATNNNNATC 1 cut(s) 2181
BseBI CCWGG 3 cut(s) 960, 1647, 2159
BseCI ATCGAT 1 cut(s) 633
BseDI CCNNGG 3 cut(s) 950, 959, 2958
BseJI GATNNNNATC 1 cut(s) 2181
BseLI CCNNNNNNNGG 6 cut(s) 610, 781, 848, 2096, 2097, 2587
BseMI GCAATG 2 cut(s) 1604, 1997
BseMII CTCAG 4 cut(s) 1460, 1674, 2078, 2271
BseNI ACTGG 7 cut(s) 676, 1308, 1553, 2169, 2245, 2429, 2707
BseRI GAGGAG 3 cut(s) 60, 1591, 1657
BseSI GKGCMC 1 cut(s) 1411
BseXI GCAGC 7 cut(s) 260, 1350, 1437, 1961, 2065, 2350, 2930
BseYI CCCAGC 3 cut(s) 604, 775, 2581
BsgI GTGCAG 1 cut(s) 2817
BshFI GGCC 5 cut(s) 958, 1196, 1409, 2998, 3044
BshTI ACCGGT 1 cut(s) 740
BshVI ATCGAT 1 cut(s) 633
BsiHKAI GWGCWC 2 cut(s) 1159, 2764
BsiHKCI CYCGRG 1 cut(s) 2510
BsiSI CCGG 4 cut(s) 627, 741, 3040, 3096
BslFI GGGAC 3 cut(s) 232, 876, 2211
BslI CCNNNNNNNGG 6 cut(s) 610, 781, 848, 2096, 2097, 2587
BsmAI GTCTC 4 cut(s) 648, 1174, 2067, 2955
BsmFI GGGAC 3 cut(s) 232, 876, 2211
BsmI GAATGC 3 cut(s) 250, 359, 667
BsnI GGCC 5 cut(s) 958, 1196, 1409, 2998, 3044
Bso31I GGTCTC 1 cut(s) 648
BsoBI CYCGRG 1 cut(s) 2510
Bsp120I GGGCCC 1 cut(s) 1407
Bsp1286I GDGCHC 4 cut(s) 1159, 1411, 2622, 2764
Bsp1407I TGTACA 1 cut(s) 1049
Bsp143I GATC 7 cut(s) 919, 973, 1245, 1718, 2182, 2647, 2925
Bsp19I CCATGG 1 cut(s) 950
BspACI CCGC 8 cut(s) 593, 1356, 1808, 2708, 2784, 2916, 3061, 3149
BspANI GGCC 5 cut(s) 958, 1196, 1409, 2998, 3044
BspCNI CTCAG 4 cut(s) 1459, 1675, 2077, 2272
BspDI ATCGAT 1 cut(s) 633
BspLI GGNNCC 8 cut(s) 363, 392, 676, 840, 892, 944, 1409, 2163
BspMAI CTGCAG 2 cut(s) 1960, 3217
BspMI ACCTGC 1 cut(s) 1963
BspPI GGATC 4 cut(s) 968, 1726, 2190, 2642
BspTNI GGTCTC 1 cut(s) 648
BsrDI GCAATG 2 cut(s) 1604, 1997
BsrFI RCCGGY 2 cut(s) 626, 740
BsrGI TGTACA 1 cut(s) 1049
BsrI ACTGG 7 cut(s) 676, 1308, 1553, 2169, 2245, 2429, 2707
BssAI RCCGGY 2 cut(s) 626, 740
BssECI CCNNGG 3 cut(s) 950, 959, 2958
BssMI GATC 7 cut(s) 919, 973, 1245, 1718, 2182, 2647, 2925
BssT1I CCWWGG 1 cut(s) 950
Bst2UI CCWGG 3 cut(s) 960, 1647, 2159
Bst6I CTCTTC 5 cut(s) 102, 462, 489, 1276, 2958
BstAPI GCANNNNNTGC 1 cut(s) 1955
BstAUI TGTACA 1 cut(s) 1049
BstBAI YACGTR 1 cut(s) 2615
BstC8I GCNNGC 7 cut(s) 667, 2216, 2368, 2502, 2724, 2799, 2880
BstDEI CTNAG 7 cut(s) 291, 314, 1446, 1683, 2064, 2280, 2765
BstDSI CCRYGG 1 cut(s) 950
BstHHI GCGC 1 cut(s) 2220
BstKTI GATC 7 cut(s) 922, 976, 1248, 1721, 2185, 2650, 2928
BstMAI GTCTC 4 cut(s) 648, 1174, 2067, 2955
BstMBI GATC 7 cut(s) 919, 973, 1245, 1718, 2182, 2647, 2925
BstMWI GCNNNNNNNGC 6 cut(s) 248, 534, 1352, 1955, 2065, 3080
BstNI CCWGG 3 cut(s) 960, 1647, 2159
BstNSI RCATGY 3 cut(s) 1278, 2110, 2385
BstSCI CCNGG 5 cut(s) 958, 1645, 2157, 3039, 3095
BstSFI CTRYAG 6 cut(s) 162, 366, 570, 1897, 1956, 3213
BstSLI GKGCMC 1 cut(s) 1411
BstV1I GCAGC 7 cut(s) 260, 1350, 1437, 1961, 2065, 2350, 2930
BstV2I GAAGAC 1 cut(s) 3038
BstX2I RGATCY 1 cut(s) 2647
BstYI RGATCY 1 cut(s) 2647
Bsu15I ATCGAT 1 cut(s) 633
BsuI GTATCC 2 cut(s) 2473, 3156
BsuRI GGCC 5 cut(s) 958, 1196, 1409, 2998, 3044
BsuTUI ATCGAT 1 cut(s) 633
BtgI CCRYGG 1 cut(s) 950
BtsI GCAGTG 5 cut(s) 1845, 2580, 2721, 2877, 3097
BveI ACCTGC 1 cut(s) 1963
Cac8I GCNNGC 7 cut(s) 667, 2216, 2368, 2502, 2724, 2799, 2880
CaiI CAGNNNCTG 3 cut(s) 578, 1955, 1964
CfoI GCGC 1 cut(s) 2220
Cfr10I RCCGGY 2 cut(s) 626, 740
Cfr13I GGNCC 7 cut(s) 158, 890, 1407, 1408, 2996, 3042, 3116
ClaI ATCGAT 1 cut(s) 633
CsiI ACCWGGT 1 cut(s) 1645
Csp6I GTAC 6 cut(s) 234, 378, 1050, 2101, 2612, 3108
CspAI ACCGGT 1 cut(s) 740
CviQI GTAC 6 cut(s) 234, 378, 1050, 2101, 2612, 3108
DdeI CTNAG 7 cut(s) 291, 314, 1446, 1683, 2064, 2280, 2765
DpnI GATC 7 cut(s) 921, 975, 1247, 1720, 2184, 2649, 2927
DpnII GATC 7 cut(s) 919, 973, 1245, 1718, 2182, 2647, 2925
DraIII CACNNNGTG 1 cut(s) 1307
DriI GACNNNNNGTC 1 cut(s) 2947
Eam1104I CTCTTC 5 cut(s) 102, 462, 489, 1276, 2958
Eam1105I GACNNNNNGTC 1 cut(s) 2947
EarI CTCTTC 5 cut(s) 102, 462, 489, 1276, 2958
Ecl136II GAGCTC 1 cut(s) 2762
Eco130I CCWWGG 1 cut(s) 950
Eco24I GRGCYC 3 cut(s) 1411, 2622, 2764
Eco31I GGTCTC 1 cut(s) 648
Eco47I GGWCC 3 cut(s) 158, 890, 3116
Eco53kI GAGCTC 1 cut(s) 2762
Eco57I CTGAAG 3 cut(s) 963, 1986, 2444
Eco88I CYCGRG 1 cut(s) 2510
EcoICRI GAGCTC 1 cut(s) 2762
EcoO109I RGGNCCY 1 cut(s) 158
EcoRI GAATTC 1 cut(s) 1133
EcoRII CCWGG 3 cut(s) 958, 1645, 2157
EcoT14I CCWWGG 1 cut(s) 950
EcoT22I ATGCAT 1 cut(s) 3211
EcoT38I GRGCYC 3 cut(s) 1411, 2622, 2764
ErhI CCWWGG 1 cut(s) 950
FalI AAGNNNNNCTT 6 cut(s) 1533, 1565, 2790, 2822, 3151, 3183
FaqI GGGAC 3 cut(s) 232, 876, 2211
FauI CCCGC 1 cut(s) 2923
FblI GTMKAC 1 cut(s) 1697
Fnu4HI GCNGC 9 cut(s) 249, 1356, 1364, 1451, 1950, 2079, 2364, 2919, 3062
FriOI GRGCYC 3 cut(s) 1411, 2622, 2764
Fsp4HI GCNGC 9 cut(s) 249, 1356, 1364, 1451, 1950, 2079, 2364, 2919, 3062
FspBI CTAG 3 cut(s) 701, 923, 3231
GlaI GCGC 1 cut(s) 2219
GluI GCNGC 9 cut(s) 249, 1356, 1364, 1451, 1950, 2079, 2364, 2919, 3062
GsaI CCCAGC 3 cut(s) 608, 779, 2585
GsuI CTGGAG 3 cut(s) 165, 1650, 2778
HaeIII GGCC 5 cut(s) 958, 1196, 1409, 2998, 3044
HapII CCGG 4 cut(s) 627, 741, 3040, 3096
HhaI GCGC 1 cut(s) 2220
Hin6I GCGC 1 cut(s) 2218
HinP1I GCGC 1 cut(s) 2218
HindIII AAGCTT 3 cut(s) 912, 3081, 3165
HpaII CCGG 4 cut(s) 627, 741, 3040, 3096
HphI GGTGA 8 cut(s) 95, 149, 755, 1304, 1822, 2631, 3147, 3233
Hpy166II GTNNAC 4 cut(s) 698, 1301, 1698, 2578
Hpy8I GTNNAC 4 cut(s) 698, 1301, 1698, 2578
HpyCH4IV ACGT 3 cut(s) 555, 713, 2614
HpyF10VI GCNNNNNNNGC 6 cut(s) 248, 534, 1352, 1955, 2065, 3080
HpyF3I CTNAG 7 cut(s) 291, 314, 1446, 1683, 2064, 2280, 2765
HpySE526I ACGT 3 cut(s) 555, 713, 2614
HspAI GCGC 1 cut(s) 2218
Kzo9I GATC 7 cut(s) 919, 973, 1245, 1718, 2182, 2647, 2925
Lsp1109I GCAGC 7 cut(s) 260, 1350, 1437, 1961, 2065, 2350, 2930
MabI ACCWGGT 1 cut(s) 1645
MaeI CTAG 3 cut(s) 701, 923, 3231
MaeII ACGT 3 cut(s) 555, 713, 2614
MaeIII GTNAC 6 cut(s) 199, 317, 1396, 1675, 2394, 2637
MalI GATC 7 cut(s) 921, 975, 1247, 1720, 2184, 2649, 2927
MboI GATC 7 cut(s) 919, 973, 1245, 1718, 2182, 2647, 2925
MfeI CAATTG 1 cut(s) 2186
MflI RGATCY 1 cut(s) 2647
MhlI GDGCHC 4 cut(s) 1159, 1411, 2622, 2764
MlyI GAGTC 9 cut(s) 214, 647, 1380, 1796, 2294, 2338, 2402, 2645, 2957
MmeI TCCRAC 5 cut(s) 562, 696, 1412, 1864, 2265
Mph1103I ATGCAT 1 cut(s) 3211
MspA1I CMGCKG 3 cut(s) 608, 1450, 2786
MspI CCGG 4 cut(s) 627, 741, 3040, 3096
MspR9I CCNGG 5 cut(s) 960, 1647, 2159, 3041, 3097
MunI CAATTG 1 cut(s) 2186
Mva1269I GAATGC 3 cut(s) 250, 359, 667
MvaI CCWGG 3 cut(s) 960, 1647, 2159
MwoI GCNNNNNNNGC 6 cut(s) 248, 534, 1352, 1955, 2065, 3080
NciI CCSGG 2 cut(s) 3041, 3097
NcoI CCATGG 1 cut(s) 950
NdeII GATC 7 cut(s) 919, 973, 1245, 1718, 2182, 2647, 2925
NlaIV GGNNCC 8 cut(s) 363, 392, 676, 840, 892, 944, 1409, 2163
NmuCI GTSAC 4 cut(s) 199, 317, 2394, 2637
NsiI ATGCAT 1 cut(s) 3211
NspI RCATGY 3 cut(s) 1278, 2110, 2385
PaeR7I CTCGAG 1 cut(s) 2510
PaqCI CACCTGC 1 cut(s) 1963
PciI ACATGT 1 cut(s) 2381
PctI GAATGC 3 cut(s) 250, 359, 667
PinAI ACCGGT 1 cut(s) 740
PkrI GCNGC 9 cut(s) 250, 1357, 1365, 1452, 1951, 2080, 2365, 2920, 3063
PleI GAGTC 9 cut(s) 214, 646, 1380, 1796, 2294, 2337, 2401, 2644, 2956
PpsI GAGTC 9 cut(s) 214, 646, 1380, 1796, 2294, 2337, 2401, 2644, 2956
Ppu21I YACGTR 1 cut(s) 2615
PpuMI RGGWCCY 1 cut(s) 158
PscI ACATGT 1 cut(s) 2381
PshBI ATTAAT 1 cut(s) 1251
Psp124BI GAGCTC 1 cut(s) 2764
Psp5II RGGWCCY 1 cut(s) 158
Psp6I CCWGG 3 cut(s) 958, 1645, 2157
PspFI CCCAGC 3 cut(s) 604, 775, 2581
PspGI CCWGG 3 cut(s) 958, 1645, 2157
PspN4I GGNNCC 8 cut(s) 363, 392, 676, 840, 892, 944, 1409, 2163
PspOMI GGGCCC 1 cut(s) 1407
PspPI GGNCC 7 cut(s) 158, 890, 1407, 1408, 2996, 3042, 3116
PspPPI RGGWCCY 1 cut(s) 158
PstI CTGCAG 2 cut(s) 1960, 3217
PstNI CAGNNNCTG 3 cut(s) 578, 1955, 1964
PsuI RGATCY 1 cut(s) 2647
PvuII CAGCTG 2 cut(s) 608, 1450
RsaI GTAC 6 cut(s) 235, 379, 1051, 2102, 2613, 3109
RsaNI GTAC 6 cut(s) 234, 378, 1050, 2101, 2612, 3108
SacI GAGCTC 1 cut(s) 2764
SatI GCNGC 9 cut(s) 249, 1356, 1364, 1451, 1950, 2079, 2364, 2919, 3062
Sau3AI GATC 7 cut(s) 919, 973, 1245, 1718, 2182, 2647, 2925
Sau96I GGNCC 7 cut(s) 158, 890, 1407, 1408, 2996, 3042, 3116
SchI GAGTC 9 cut(s) 214, 647, 1380, 1796, 2294, 2338, 2402, 2645, 2957
ScrFI CCNGG 5 cut(s) 960, 1647, 2159, 3041, 3097
SduI GDGCHC 4 cut(s) 1159, 1411, 2622, 2764
SexAI ACCWGGT 1 cut(s) 1645
SfcI CTRYAG 6 cut(s) 162, 366, 570, 1897, 1956, 3213
Sfr274I CTCGAG 1 cut(s) 2510
SinI GGWCC 3 cut(s) 158, 890, 3116
SlaI CTCGAG 1 cut(s) 2510
SmlI CTYRAG 5 cut(s) 1171, 1759, 2510, 2807, 3140
SmoI CTYRAG 5 cut(s) 1171, 1759, 2510, 2807, 3140
SsiI CCGC 8 cut(s) 593, 1356, 1808, 2708, 2784, 2916, 3061, 3149
SspI AATATT 3 cut(s) 1935, 2205, 2980
SspMI CTAG 3 cut(s) 701, 923, 3231
SstI GAGCTC 1 cut(s) 2764
StyD4I CCNGG 5 cut(s) 958, 1645, 2157, 3039, 3095
StyI CCWWGG 1 cut(s) 950
TaiI ACGT 3 cut(s) 558, 716, 2617
TaqI TCGA 5 cut(s) 278, 398, 633, 1389, 2511
TatI WGTACW 1 cut(s) 1049
TauI GCSGC 2 cut(s) 1358, 3064
TscAI CASTG 9 cut(s) 676, 1308, 1845, 2427, 2580, 2721, 2841, 2877, 3097
TseFI GTSAC 4 cut(s) 199, 317, 2394, 2637
TseI GCWGC 7 cut(s) 248, 1363, 1450, 1949, 2078, 2363, 2918
Tsp45I GTSAC 4 cut(s) 199, 317, 2394, 2637
TspRI CASTG 9 cut(s) 676, 1308, 1845, 2427, 2580, 2721, 2841, 2877, 3097
VpaK11BI GGWCC 3 cut(s) 158, 890, 3116
VspI ATTAAT 1 cut(s) 1251
XapI RAATTY 4 cut(s) 905, 1133, 2138, 2840
XceI RCATGY 3 cut(s) 1278, 2110, 2385
XhoI CTCGAG 1 cut(s) 2510
XmiI GTMKAC 1 cut(s) 1697
XspI CTAG 3 cut(s) 701, 923, 3231
Zsp2I ATGCAT 1 cut(s) 3211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.