RLG00000024413

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002940
Physical Location & Seq
Reverse (-)
35595846 .. 35597441
1596 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024413

Sequence Viewer

Length: 1596 bp
ATGGATACTGACCGTGGAGCTGAATTTGGTGAAGGGTTGGTTTTTGATGCCAAGGATAGTTGTAAGAATGTTGAAAGTTCGTTAATTTCGGATAAAGATAGAGTAAAAGATGAGACTTTGAGGGAAAGGATGGAAGGTAAAGTTGTGAGTGCCGGGGGAGTGGAATTTAATAATAACAATGAGTCTTTGGATTATCAAGACTTCAATGCTAGCGGCTGTTTTAAGATGGTAGATTGTGATGGAGTTGAAAATGAGGCCTTGGAAGTTAATGTGGAAGAAGAGAGTAATCGAAATGAATTGACGCCGATGAGGGTCTCTGTGAGTACCGAATGCGAGGTTGATTTGCTCAGTGTTGCTGATTTTATCCGGTATTCGAGAATGGATGGGAATGTGTCAAGTGGTGTGAATGGAAAATCTTGTTCTTCCAAGCTAGAATTTGAAGTTTCTGATCTGGTATGGGGCAAAGTAAGGACTCATCCCTGGTGGCCCGGGCAGATTTGTGATCCTTCGGCTGCGTCAGAACTGGCAAAGAAGTGTTTTAAGAGGGGTAGTTATCTGATAGCTTACTTTGGGGATCAGACATTTGCTTGGAATGAAGCACAAAAGATTAAGCCCTTTCTGGAGCACTGCTCAAAAATGGAGAAGCAGAGTGATATGGAAGAATTCCAAAACGCAGTTGGTTGTGCGTTGGACGAAGTGTCGAGAAGGGTAGAGTTTGGGCTGGCCTGTTGTTGCATATCCAAACGAGACTACGCAAGGCTCAAAACTCAGAAGATTATTAATGCTGGAATCAGGAAAGAAGCAGCAAATAGAAGAGATGGTGGTGACAGTTCTCTTAGCGCCGCTTTTTTTGAACCTGCGAAAGTCATAAAGTTTATAAAAAAGTTGGCTCAGTTGCCATATGGCAAAGCTGACAGACTAGAACTTGTAACATCTCTAGCCCAGCTGTCAGCTTTCTGGCGTTGGAAGAGTTATGTGCCTCTGCCAGAGGAGAATGATACTGATATTTTCCTATTGGGAGAGAAGAACCACTGCAATGAAGTAAGTGATGAAGCACTCTCAGTACTTGAGAATGAGAAGTTGGTGCAAAACTCAGATAGTAAAGAGAACACATTACGTGAGTGCAAGGACATATCAGATTCAAAAGCTCAGAGGTGTTTGTTTCCAATGAGTGAGAATGGATCAGAAGGGAAAGCTGGCAGTGAATTGATTATGCAACCTTGCAACAAAAGAAAGGGTCTTGATGCTATAGCTGATGAAGATGTACTCCCAGTAATCAAAGATAACTCATTGCATAAGTGTCGGCACATATCTGAACATAGTATGAATCTGAGTGAAGAAGAGCAAAGCTTGTCAGATTTGGAGGGTACGAAGTGTTTGTCTACTCCAGTGAGTGAGAATGGATTAGAAGAGAAAGGTGGTGGTAAATTGATTTCACAACCTTCTGTTAAGAAACGAAAGGAAGTTGATGCTATGGCTGATGATGATGCTGTGAAACATAGGAAAAGTGATTCATCACAAGGGATTGGTAGCAATGCGAACTGTCAGTCATTTGAAGTTGAAGACTTTTTTAACCTGTTTTTACAGCAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

532

Amino Acids

59.24

Weight (kDa)

4.92

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 150 - 232 6e-19 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 878
Acc36I ACCTGC 1 cut(s) 865
AccI GTMKAC 1 cut(s) 1382
AciI CCGC 2 cut(s) 213, 843
AclWI GGATC 3 cut(s) 497, 582, 1189
AcsI RAATTY 4 cut(s) 23, 164, 434, 662
AcyI GRCGYC 1 cut(s) 302
AfaI GTAC 4 cut(s) 325, 1065, 1266, 1369
AgsI TTSAA 8 cut(s) 74, 205, 248, 440, 854, 1143, 1556, 1562
AhdI GACNNNNNGTC 1 cut(s) 697
AjnI CCWGG 1 cut(s) 479
AjuI GAANNNNNNNTTGG 2 cut(s) 1064, 1096
Alw21I GWGCWC 1 cut(s) 627
Alw26I GTCTC 3 cut(s) 107, 319, 741
AlwI GGATC 3 cut(s) 497, 582, 1189
Ama87I CYCGRG 1 cut(s) 488
AoxI GGCC 3 cut(s) 255, 485, 723
ApeKI GCWGC 2 cut(s) 512, 803
ApoI RAATTY 4 cut(s) 23, 164, 434, 662
ArsI GACNNNNNNTTYG 4 cut(s) 1364, 1396, 1532, 1564
AseI ATTAAT 1 cut(s) 780
AspLEI GCGC 1 cut(s) 842
AspS9I GGNCC 1 cut(s) 486
AsuC2I CCSGG 3 cut(s) 154, 489, 490
AsuHPI GGTGA 2 cut(s) 41, 836
AsuNHI GCTAGC 1 cut(s) 209
AvaI CYCGRG 1 cut(s) 488
BbsI GAAGAC 1 cut(s) 1569
Bbv12I GWGCWC 1 cut(s) 627
BbvI GCAGC 2 cut(s) 499, 815
BccI CCATC 5 cut(s) 124, 220, 233, 377, 812
BcgI CGANNNNNNTGC 2 cut(s) 1283, 1317
BciT130I CCWGG 1 cut(s) 481
BcnI CCSGG 3 cut(s) 154, 489, 490
BcoDI GTCTC 3 cut(s) 107, 319, 741
BfaI CTAG 4 cut(s) 210, 431, 920, 938
BfmI CTRYAG 1 cut(s) 1248
BfoI RGCGCY 1 cut(s) 843
BfuAI ACCTGC 1 cut(s) 865
BisI GCNGC 4 cut(s) 214, 513, 804, 843
BlsI GCNGC 4 cut(s) 215, 514, 805, 844
BmcAI AGTACT 1 cut(s) 1065
Bme1390I CCNGG 4 cut(s) 154, 481, 489, 490
BmeRI GACNNNNNGTC 1 cut(s) 697
BmeT110I CYCGRG 1 cut(s) 488
BmgT120I GGNCC 1 cut(s) 486
BmrFI CCNGG 4 cut(s) 154, 481, 489, 490
BmrI ACTGGG 1 cut(s) 1265
BmsI GCATC 4 cut(s) 37, 1234, 1459, 1477
BmtI GCTAGC 1 cut(s) 213
BmuI ACTGGG 1 cut(s) 1265
BpiI GAAGAC 1 cut(s) 1569
BplI GAGNNNNNCTC 2 cut(s) 614, 646
BpmI CTGGAG 2 cut(s) 641, 1371
BpuEI CTTGAG 1 cut(s) 1088
BpuMI CCSGG 3 cut(s) 154, 489, 490
BsaAI YACGTR 1 cut(s) 1118
BsaHI GRCGYC 1 cut(s) 302
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 6 cut(s) 13, 51, 153, 258, 479, 488
BsaWI WCCGGW 1 cut(s) 366
Bse1I ACTGG 3 cut(s) 528, 1271, 1388
Bse3DI GCAATG 3 cut(s) 1042, 1289, 1540
BseBI CCWGG 1 cut(s) 481
BseDI CCNNGG 6 cut(s) 13, 51, 153, 258, 479, 488
BseGI GGATG 3 cut(s) 135, 388, 475
BseMI GCAATG 3 cut(s) 1042, 1289, 1540
BseMII CTCAG 7 cut(s) 361, 782, 905, 1074, 1107, 1163, 1322
BseNI ACTGG 3 cut(s) 528, 1271, 1388
BseRI GAGGAG 1 cut(s) 1004
BseXI GCAGC 2 cut(s) 499, 815
BseYI CCCAGC 1 cut(s) 942
BshFI GGCC 3 cut(s) 257, 487, 725
BsiHKAI GWGCWC 1 cut(s) 627
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 3 cut(s) 153, 367, 489
BsmAI GTCTC 3 cut(s) 107, 319, 741
BsmI GAATGC 1 cut(s) 335
BsnI GGCC 3 cut(s) 257, 487, 725
Bso31I GGTCTC 1 cut(s) 319
BsoBI CYCGRG 1 cut(s) 488
Bsp1286I GDGCHC 1 cut(s) 627
Bsp143I GATC 4 cut(s) 448, 502, 574, 1181
BspACI CCGC 2 cut(s) 213, 843
BspANI GGCC 3 cut(s) 257, 487, 725
BspCNI CTCAG 7 cut(s) 360, 781, 904, 1073, 1106, 1162, 1323
BspMI ACCTGC 1 cut(s) 865
BspOI GCTAGC 1 cut(s) 213
BspPI GGATC 3 cut(s) 497, 582, 1189
BspQI GCTCTTC 1 cut(s) 1335
BspTNI GGTCTC 1 cut(s) 319
BsrDI GCAATG 3 cut(s) 1042, 1289, 1540
BsrI ACTGG 3 cut(s) 528, 1271, 1388
BssECI CCNNGG 6 cut(s) 13, 51, 153, 258, 479, 488
BssMI GATC 4 cut(s) 448, 502, 574, 1181
BssNI GRCGYC 1 cut(s) 302
BssT1I CCWWGG 2 cut(s) 51, 258
Bst2UI CCWGG 1 cut(s) 481
Bst4CI ACNGT 3 cut(s) 14, 830, 1544
Bst6I CTCTTC 5 cut(s) 273, 808, 962, 1335, 1404
BstACI GRCGYC 1 cut(s) 302
BstBAI YACGTR 1 cut(s) 1118
BstC8I GCNNGC 3 cut(s) 211, 723, 1198
BstDEI CTNAG 8 cut(s) 347, 768, 836, 891, 1060, 1093, 1149, 1331
BstDSI CCRYGG 1 cut(s) 13
BstF5I GGATG 3 cut(s) 135, 388, 475
BstH2I RGCGCY 1 cut(s) 843
BstHHI GCGC 1 cut(s) 842
BstKTI GATC 4 cut(s) 451, 505, 577, 1184
BstMAI GTCTC 3 cut(s) 107, 319, 741
BstMBI GATC 4 cut(s) 448, 502, 574, 1181
BstNI CCWGG 1 cut(s) 481
BstSCI CCNGG 4 cut(s) 152, 479, 487, 488
BstSFI CTRYAG 1 cut(s) 1248
BstV1I GCAGC 2 cut(s) 499, 815
BstV2I GAAGAC 1 cut(s) 1569
BsuRI GGCC 3 cut(s) 257, 487, 725
BtgI CCRYGG 1 cut(s) 13
BtsCI GGATG 3 cut(s) 135, 388, 475
BtsI GCAGTG 3 cut(s) 625, 1030, 1207
BtsIMutI CAGTG 5 cut(s) 355, 625, 1030, 1207, 1395
BveI ACCTGC 1 cut(s) 865
Cac8I GCNNGC 3 cut(s) 211, 723, 1198
CfoI GCGC 1 cut(s) 842
Cfr13I GGNCC 1 cut(s) 486
Cfr9I CCCGGG 1 cut(s) 488
CseI GACGC 2 cut(s) 310, 504
Csp6I GTAC 4 cut(s) 324, 1064, 1265, 1368
CviQI GTAC 4 cut(s) 324, 1064, 1265, 1368
DdeI CTNAG 8 cut(s) 347, 768, 836, 891, 1060, 1093, 1149, 1331
DpnI GATC 4 cut(s) 450, 504, 576, 1183
DpnII GATC 4 cut(s) 448, 502, 574, 1181
DriI GACNNNNNGTC 1 cut(s) 697
Eam1104I CTCTTC 5 cut(s) 273, 808, 962, 1335, 1404
Eam1105I GACNNNNNGTC 1 cut(s) 697
EarI CTCTTC 5 cut(s) 273, 808, 962, 1335, 1404
Eco130I CCWWGG 2 cut(s) 51, 258
Eco147I AGGCCT 1 cut(s) 257
Eco31I GGTCTC 1 cut(s) 319
Eco88I CYCGRG 1 cut(s) 488
EcoRI GAATTC 1 cut(s) 662
EcoRII CCWGG 1 cut(s) 479
EcoT14I CCWWGG 2 cut(s) 51, 258
ErhI CCWWGG 2 cut(s) 51, 258
FauNDI CATATG 1 cut(s) 901
FblI GTMKAC 1 cut(s) 1382
Fnu4HI GCNGC 4 cut(s) 214, 513, 804, 843
FokI GGATG 3 cut(s) 142, 395, 462
Fsp4HI GCNGC 4 cut(s) 214, 513, 804, 843
FspBI CTAG 4 cut(s) 210, 431, 920, 938
GlaI GCGC 1 cut(s) 841
GluI GCNGC 4 cut(s) 214, 513, 804, 843
GsaI CCCAGC 1 cut(s) 946
GsuI CTGGAG 2 cut(s) 641, 1371
HaeII RGCGCY 1 cut(s) 843
HaeIII GGCC 3 cut(s) 257, 487, 725
HapII CCGG 3 cut(s) 153, 367, 489
HgaI GACGC 2 cut(s) 310, 504
HhaI GCGC 1 cut(s) 842
Hin1I GRCGYC 1 cut(s) 302
Hin6I GCGC 1 cut(s) 840
HinP1I GCGC 1 cut(s) 840
HindIII AAGCTT 1 cut(s) 1348
HinfI GANTC 6 cut(s) 182, 472, 789, 1139, 1327, 1511
HpaII CCGG 3 cut(s) 153, 367, 489
HphI GGTGA 2 cut(s) 41, 836
Hpy166II GTNNAC 1 cut(s) 1383
Hpy188III TCNNGA 6 cut(s) 197, 375, 620, 702, 793, 1241
Hpy8I GTNNAC 1 cut(s) 1383
HpyAV CCTTC 6 cut(s) 26, 128, 516, 699, 1181, 1452
HpyCH4III ACNGT 3 cut(s) 14, 830, 1544
HpyCH4IV ACGT 1 cut(s) 1117
HpyCH4V TGCA 7 cut(s) 735, 1035, 1087, 1125, 1216, 1224, 1294
HpyF3I CTNAG 8 cut(s) 347, 768, 836, 891, 1060, 1093, 1149, 1331
HpySE526I ACGT 1 cut(s) 1117
Hsp92I GRCGYC 1 cut(s) 302
HspAI GCGC 1 cut(s) 840
Kzo9I GATC 4 cut(s) 448, 502, 574, 1181
LguI GCTCTTC 1 cut(s) 1335
LmnI GCTCC 2 cut(s) 17, 622
Lsp1109I GCAGC 2 cut(s) 499, 815
LweI GCATC 4 cut(s) 37, 1234, 1459, 1477
MaeI CTAG 4 cut(s) 210, 431, 920, 938
MaeII ACGT 1 cut(s) 1117
MaeIII GTNAC 2 cut(s) 824, 928
MalI GATC 4 cut(s) 450, 504, 576, 1183
MboI GATC 4 cut(s) 448, 502, 574, 1181
MhlI GDGCHC 1 cut(s) 627
MluCI AATT 8 cut(s) 23, 84, 164, 296, 434, 662, 1205, 1427
MlyI GAGTC 2 cut(s) 191, 466
MmeI TCCRAC 2 cut(s) 669, 944
MnlI CCTC 9 cut(s) 114, 247, 303, 328, 537, 982, 990, 1146, 1357
MseI TTAA 9 cut(s) 83, 168, 222, 267, 540, 609, 780, 1449, 1572
MslI CAYNNNNRTG 1 cut(s) 1035
MspA1I CMGCKG 1 cut(s) 946
MspI CCGG 3 cut(s) 153, 367, 489
MspR9I CCNGG 4 cut(s) 154, 481, 489, 490
Mva1269I GAATGC 1 cut(s) 335
MvaI CCWGG 1 cut(s) 481
NciI CCSGG 3 cut(s) 154, 489, 490
NdeI CATATG 1 cut(s) 901
NdeII GATC 4 cut(s) 448, 502, 574, 1181
NheI GCTAGC 1 cut(s) 209
NmuCI GTSAC 1 cut(s) 824
PceI AGGCCT 1 cut(s) 257
PciSI GCTCTTC 1 cut(s) 1335
PctI GAATGC 1 cut(s) 335
PfeI GAWTC 4 cut(s) 789, 1139, 1327, 1511
PkrI GCNGC 4 cut(s) 215, 514, 805, 844
PleI GAGTC 2 cut(s) 190, 466
PpsI GAGTC 2 cut(s) 190, 466
Ppu21I YACGTR 1 cut(s) 1118
PshBI ATTAAT 1 cut(s) 780
PsiI TTATAA 1 cut(s) 878
Psp6I CCWGG 1 cut(s) 479
PspFI CCCAGC 1 cut(s) 942
PspGI CCWGG 1 cut(s) 479
PspPI GGNCC 1 cut(s) 486
PvuII CAGCTG 1 cut(s) 946
RsaI GTAC 4 cut(s) 325, 1065, 1266, 1369
RsaNI GTAC 4 cut(s) 324, 1064, 1265, 1368
RseI CAYNNNNRTG 1 cut(s) 1035
SapI GCTCTTC 1 cut(s) 1335
SaqAI TTAA 9 cut(s) 83, 168, 222, 267, 540, 609, 780, 1449, 1572
SatI GCNGC 4 cut(s) 214, 513, 804, 843
Sau3AI GATC 4 cut(s) 448, 502, 574, 1181
Sau96I GGNCC 1 cut(s) 486
ScaI AGTACT 1 cut(s) 1065
SchI GAGTC 2 cut(s) 191, 466
ScrFI CCNGG 4 cut(s) 154, 481, 489, 490
SduI GDGCHC 1 cut(s) 627
SfaNI GCATC 4 cut(s) 37, 1234, 1459, 1477
SfcI CTRYAG 1 cut(s) 1248
SmaI CCCGGG 1 cut(s) 490
SmiMI CAYNNNNRTG 1 cut(s) 1035
SmlI CTYRAG 1 cut(s) 1067
SmoI CTYRAG 1 cut(s) 1067
SrfI GCCCGGGC 1 cut(s) 490
Sse9I AATT 8 cut(s) 23, 84, 164, 296, 434, 662, 1205, 1427
SseBI AGGCCT 1 cut(s) 257
SsiI CCGC 2 cut(s) 213, 843
SspMI CTAG 4 cut(s) 210, 431, 920, 938
StuI AGGCCT 1 cut(s) 257
StyD4I CCNGG 4 cut(s) 152, 479, 487, 488
StyI CCWWGG 2 cut(s) 51, 258
TaaI ACNGT 3 cut(s) 14, 830, 1544
TaiI ACGT 1 cut(s) 1120
TaqI TCGA 3 cut(s) 289, 374, 701
TasI AATT 8 cut(s) 23, 84, 164, 296, 434, 662, 1205, 1427
TatI WGTACW 2 cut(s) 1063, 1264
TauI GCSGC 2 cut(s) 216, 845
TfiI GAWTC 4 cut(s) 789, 1139, 1327, 1511
Tru1I TTAA 9 cut(s) 83, 168, 222, 267, 540, 609, 780, 1449, 1572
Tru9I TTAA 9 cut(s) 83, 168, 222, 267, 540, 609, 780, 1449, 1572
TscAI CASTG 5 cut(s) 355, 632, 1037, 1207, 1395
TseFI GTSAC 1 cut(s) 824
TseI GCWGC 2 cut(s) 512, 803
Tsp45I GTSAC 1 cut(s) 824
TspDTI ATGAA 7 cut(s) 309, 609, 1053, 1065, 1272, 1340, 1503
TspMI CCCGGG 1 cut(s) 488
TspRI CASTG 5 cut(s) 355, 632, 1037, 1207, 1395
VspI ATTAAT 1 cut(s) 780
XapI RAATTY 4 cut(s) 23, 164, 434, 662
XcmI CCANNNNNNNNNTGG 1 cut(s) 674
XmaI CCCGGG 1 cut(s) 488
XmiI GTMKAC 1 cut(s) 1382
XspI CTAG 4 cut(s) 210, 431, 920, 938
ZrmI AGTACT 1 cut(s) 1065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.