FvH4_6g15320

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002940
Physical Location & Seq
Forward (+)
9534672 .. 9538564
3893 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g15320.t2

Sequence Viewer

Length: 3291 bp
ATGGCTCGGATAAGGAAGCAGGGGGTTAATTTCTCCGGGGAGGGAGGAGTTGAGAGTGTTGATAGTGATGTAGTGGGTGAGGAGAGGAGTGAGGGTTTTGAAGAGAGAGATGGTGCGGCGAAAGTAGGGCCTGTAGATATGGAGATTGATGAGAAAGGTGGAGGTGAAGAGACAGAGGGTTTGGAGGGGACTCGGGTTGGGGGTAGTGATGTGAATGCTGCAAGTGAGGAAGGGCTGGTTTCGGTGTCGAAGGAGAGTTCTAAGGAGGTTGAAGGTTCACTTTTTAGTGATAAGGATGGAGGCAGAGATGAGGTTTTGAGGGAATGTGTGGATGGGAGTCATTCGATGAGTTTAGAGGTGAAGGCGGATGTGGTAGAGGAGGGAAAGGATGAGAAAGAGGAGGAAGTTGCACGAGTAGATGAGGAAGAGAAGGAAAGAGAACAGGATGTGGAGCGTAGCCAGGTTGAAGAGAAGAGTGTCCATGTTTCAAGTTCTGATGCGGAACACGAGGCGACAGGGTCTGTTGGAGGGATAGCGCAGGACATCCCAGCTGTGGAAGTGAAAGTTACCAGTATCGATGAGTCTGTGCATGATGGAGAGCGGAATGTATGCCCTGGTTCACAGATGGCTACTTGTGGTGGACTAGAAAATCAGACGTTAGAAGTTAATGTGGGAGTAAAGACCGGTGAAGATGAATTGATGCCTGAAAATGTTTCCTCACCAGAGGATTCTGTGAAATCTGAAGATGAGAATAACTTGGTTGATATGGCTATTGATTTGGAACCATATTCTGGAAGTGAAGGGAATGAATCAGATGACATGGATGTTTCTGGTCCCTCAAAACCAGAATTTCAAGCTTTTGATCTAGTATGGGGAAAAGTTAGGAGCCATCCCTGGTGGCCTGGGCAGATATGTAATCCTTCGGATTCATCAGAACAGGCAAAAAAGTATTTTAAGAAGCGCAGTTATCTGATTGCTTATTTTTGGGATTGTACATTTGCTTGGAATGAAGCACCACAAATTAAGCCCTTTTGGGAGAACTTTTCACAAATGGAAAAGCAGAGTAATATGGAGGAATTTCAAAATGCAGTTGCTTGTGCTCTGGATGAAGTCTCAAGACGGGTAGAGTTTGGCTTGGCCTGTTCTTGCATTTCCAAAGAAGTATATGCAAAGCTCAAAACACAGACAATCGTCAATGCTGGAATCAAGGAAGAAGCAACTAGAAGAGATGGTGGTGATAGTTCTCTCGATGCTGCTTCTTTTGATCCCTTGAAACTCATTAAGTTTGCAAAACAAGCTGCCAAGTTGCCATTTGGCAGAGCTGACAAGCTAGAACTTGTTACATCACGGGCCCAATTATCATCTTTCTGTCGTTGGAAGGGTTATTCTCAGCTGCCAGAGTTCAATTTTCTTGGTGGATTGCTGGAGAATGACGCCGACATTTCACTATTGAGAGAAAAGGAGCAAGTTAATGAACTAACAGGAGATGCACTTCCAGTTATTAACGATGATGCATTGGAGAGGAAATCAAAAGGGAAAAACTCCTCATTCTATAAGCGTAAGCACTCTGAAGATAGTTCCAAACCAAGTAAAAAAGGGCAAAACTCCTCACATGTCACTGCTGAGAAGGGTTTGTCTACTCCCATGAGTGGGAATGGATCAGAAAGTAAAGCTGGTTGTAAATTGGTTTCACAATCCTCCAGTAAGAAACGAAAGGCAATTGTTGGTGCTATCTCTGATGACTCTGCGGTGAAACAAAGTAAAAATGCTTCGTTAGTGGATGATTATATTTCTCTACAGAATAAGCAGACTTTTAAAGTTGGAGATAGAATCTGTAGGGTTGCGACCCTGCTAAATGGGTCAAGTCCAATATTTAGGCTTTCCAATGCAGCACCTGCAGATGCTGAAGAGGATGAAGGCAAAGAGAAATCTACATCACAGGCACCACAAACTGAAAGGCTGATTGACATTGAGGATCCTTCTCCTGATGAGATGCTGTCTCACCTCTGCTTGGCCGCCATAAGTCCTAATGAAGGGTACAGCATGATGACATCCTTGATGGCCTGCTTCTCAAAATATAGGAATACTATATGCCTGGAACCTTCTAGTCTAGATGAGGATCAATTGCCTTTGGGACAAATATTTGGTAGCAAGCGTGGTAAGAAATCAACCAAGACTTCGAAAAAATCAATTAAACCGTGCAAATCTGAAAAATCTGAGTTGGAGCTGGTCAAAGACTTTCCTTCTGATAAGGTAGTGAAGAGTCAAAATGAAAATGGAAAGTTATTACATGGAGCTGCAAGCGACAAAGATACCTGTGTTGTAGTGTCACAATCAAGTCTGGATTCTGAAGCACAGAAGCCAGCATTTGACAATGAAAATGGAGAGTTGGTACATGGAGCTGCTACCAGCGACAAAGATACCTGTGTTATGGTGTCACAATCAAGTCTTTATTTGGAAGCACAGAAGCCAGCTTGTGACAATAAAAATGGAGAGTTGGTGCATGGAGCTGCTACCAGCGATAAGGATAATTCCACTGTTGAATCACAAGCAAGTATTGAGTTAAATCCCAATTTGGATTCTGAACAGAACATTGCTTGTGGAGATCTTGATTCAAAGGCAACCAATCCAGCTCTGGACGATGAAAAAGAAGCATTAGCACGTGAGTCCATCTCTGTTGTTGAGTTGTCAGCAGATCCCAAAGTGAATCCCAATTTGGATTTTGAACAGGAAAATACAGGCGGTGATACTGGTTCGGAAGCAGCTAAGGTAGCTCTTGACAATGAAAGTGGAGCGTTGGTTGCTGGAGCTCTAAGTAATAAAGATGGTTCCGCTAAGGAATTGCAAGAAAGTCTTGAGTTTAATCCCAATATGGACTCTGTGCTAAAATTTCCTGATGGAGATCATGATTCAGAAGCAGTGAAGCCAGCTCCCCAAAGTGAGAGTTGTGAGGAAGATTCTCCTGCAGCTTTGATCTTGAACTTTTCAGACTCGGAGTCTCTTCCCTCGGAAGAATATCTGAACAAGATTTTTAGTGGCTTTGGTCCTTTAAATGAATCGCAAACCGAATTGCTGAAGAAGACTAACCGGGCTAAAGTGGTTTTCAAGCGGCGTTCGGACGCTCAAAAAGCTTTCAGCAGTGCCGGGAAATACAGTACATTTGGACCTTCACTTGAAAGTTATCGGCTCAAGTACCCGCTATCACCAAGTAAAGCTTCTCCTTCTCCTTCTCCAAAGGTGGCAAAGCAAGGGGATGAATCTGTAGAGGGTGATACAGCTAGGAGACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1097

Amino Acids

118.65

Weight (kDa)

4.67

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 288 - 370 4.4e-17 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1903
Acc36I ACCTGC 1 cut(s) 1903
AccB1I GGYRCC 1 cut(s) 1942
AccB7I CCANNNNNTGG 1 cut(s) 791
AccBSI CCGCTC 1 cut(s) 601
AccI GTMKAC 1 cut(s) 1637
AclWI GGATC 6 cut(s) 1259, 1666, 1970, 1983, 2127, 2690
AcoI YGGCCR 1 cut(s) 2013
AcsI RAATTY 3 cut(s) 848, 1076, 2888
AcuI CTGAAG 5 cut(s) 762, 1590, 1926, 2370, 3095
AcvI CACGTG 1 cut(s) 2663
AcyI GRCGYC 1 cut(s) 1434
AfaI GTAC 5 cut(s) 994, 2039, 2394, 3157, 3194
AfiI CCNNNNNNNGG 6 cut(s) 553, 791, 1649, 1650, 2011, 2033
AflIII ACRYGT 1 cut(s) 1612
AgeI ACCGGT 1 cut(s) 683
AhdI GACNNNNNGTC 1 cut(s) 2995
AjnI CCWGG 5 cut(s) 459, 613, 893, 901, 2094
AjuI GAANNNNNNNTTGG 4 cut(s) 2699, 2731, 3199, 3231
Alw21I GWGCWC 2 cut(s) 1102, 2812
Alw26I GTCTC 5 cut(s) 164, 1117, 2004, 3003, 3277
AlwI GGATC 6 cut(s) 1259, 1666, 1970, 1983, 2127, 2690
AlwNI CAGNNNCTG 3 cut(s) 521, 1895, 1904
Ama87I CYCGRG 1 cut(s) 192
AoxI GGCC 6 cut(s) 128, 899, 1137, 1350, 2013, 2061
ApaI GGGCCC 1 cut(s) 1354
ApoI RAATTY 3 cut(s) 848, 1076, 2888
ArsI GACNNNNNNTTYG 2 cut(s) 163, 195
AsiGI ACCGGT 1 cut(s) 683
AspLEI GCGC 2 cut(s) 538, 963
AspS9I GGNCC 6 cut(s) 128, 833, 1350, 1351, 3044, 3164
AsuC2I CCSGG 3 cut(s) 37, 3089, 3145
AsuII TTCGAA 1 cut(s) 2180
AvaI CYCGRG 1 cut(s) 192
AvaII GGWCC 3 cut(s) 833, 3044, 3164
BaeGI GKGCMC 1 cut(s) 1354
BaeI ACNNNNGTAYC 4 cut(s) 2304, 2337, 2412, 2445
BamHI GGATCC 1 cut(s) 1975
BanI GGYRCC 1 cut(s) 1942
BanII GRGCYC 2 cut(s) 1354, 2812
BarI GAAGNNNNNNTAC 2 cut(s) 1753, 1785
BauI CACGAG 2 cut(s) 411, 506
BbrPI CACGTG 1 cut(s) 2663
BbsI GAAGAC 1 cut(s) 3086
Bbv12I GWGCWC 2 cut(s) 1102, 2812
BciT130I CCWGG 5 cut(s) 461, 615, 895, 903, 2096
BcnI CCSGG 3 cut(s) 37, 3089, 3145
BcoDI GTCTC 5 cut(s) 164, 1117, 2004, 3003, 3277
BfaI CTAG 7 cut(s) 644, 866, 1221, 1331, 2106, 2111, 3279
BfmI CTRYAG 6 cut(s) 132, 1796, 1834, 1896, 2964, 3261
BfuAI ACCTGC 1 cut(s) 1903
BglII AGATCT 1 cut(s) 2605
Bme1390I CCNGG 8 cut(s) 37, 461, 615, 895, 903, 2096, 3089, 3145
Bme18I GGWCC 3 cut(s) 833, 3044, 3164
BmeRI GACNNNNNGTC 1 cut(s) 2995
BmeT110I CYCGRG 1 cut(s) 192
BmgT120I GGNCC 6 cut(s) 128, 833, 1350, 1351, 3044, 3164
BmiI GGNNCC 8 cut(s) 783, 835, 887, 1352, 1944, 1977, 2100, 2830
BmrFI CCNGG 8 cut(s) 37, 461, 615, 895, 903, 2096, 3089, 3145
BmsI GCATC 7 cut(s) 487, 690, 1240, 1477, 1501, 1891, 1983
BoxI GACNNNNGTC 1 cut(s) 1190
BpiI GAAGAC 1 cut(s) 3086
BplI GAGNNNNNCTC 2 cut(s) 2657, 2689
BpmI CTGGAG 3 cut(s) 1445, 1684, 2826
Bpu10I CCTNAGC 2 cut(s) 2766, 2835
Bpu14I TTCGAA 1 cut(s) 2180
BpuEI CTTGAG 3 cut(s) 1099, 2876, 3173
BpuMI CCSGG 3 cut(s) 37, 3089, 3145
Bsa29I ATCGAT 1 cut(s) 576
BsaAI YACGTR 1 cut(s) 2663
BsaBI GATNNNNATC 2 cut(s) 2118, 2901
BsaHI GRCGYC 1 cut(s) 1434
BsaJI CCNNGG 5 cut(s) 36, 613, 893, 902, 3006
BsaWI WCCGGW 1 cut(s) 683
BsaXI ACNNNNNCTCC 8 cut(s) 79, 109, 2376, 2406, 2484, 2514, 2784, 2814
Bsc4I CCNNNNNNNGG 6 cut(s) 553, 791, 1649, 1650, 2011, 2033
Bse118I RCCGGY 1 cut(s) 683
Bse1I ACTGG 4 cut(s) 570, 1496, 1701, 2755
Bse3DI GCAATG 1 cut(s) 2592
Bse8I GATNNNNATC 2 cut(s) 2118, 2901
BseBI CCWGG 5 cut(s) 461, 615, 895, 903, 2096
BseCI ATCGAT 1 cut(s) 576
BseDI CCNNGG 5 cut(s) 36, 613, 893, 902, 3006
BseJI GATNNNNATC 2 cut(s) 2118, 2901
BseLI CCNNNNNNNGG 6 cut(s) 553, 791, 1649, 1650, 2011, 2033
BseMI GCAATG 1 cut(s) 2592
BseMII CTCAG 3 cut(s) 1403, 1614, 2208
BseNI ACTGG 4 cut(s) 570, 1496, 1701, 2755
BseRI GAGGAG 7 cut(s) 60, 95, 100, 392, 413, 1534, 1597
BseSI GKGCMC 1 cut(s) 1354
BseYI CCCAGC 1 cut(s) 547
BshFI GGCC 6 cut(s) 130, 901, 1139, 1352, 2015, 2063
BshNI GGYRCC 1 cut(s) 1942
BshTI ACCGGT 1 cut(s) 683
BshVI ATCGAT 1 cut(s) 576
BsiHKAI GWGCWC 2 cut(s) 1102, 2812
BsiHKCI CYCGRG 1 cut(s) 192
BsiSI CCGG 4 cut(s) 36, 684, 3088, 3144
BslFI GGGAC 3 cut(s) 202, 819, 2148
BslI CCNNNNNNNGG 6 cut(s) 553, 791, 1649, 1650, 2011, 2033
BsmAI GTCTC 5 cut(s) 164, 1117, 2004, 3003, 3277
BsmFI GGGAC 3 cut(s) 202, 819, 2148
BsmI GAATGC 1 cut(s) 220
BsnI GGCC 6 cut(s) 130, 901, 1139, 1352, 2015, 2063
BsoBI CYCGRG 1 cut(s) 192
Bsp119I TTCGAA 1 cut(s) 2180
Bsp120I GGGCCC 1 cut(s) 1350
Bsp1286I GDGCHC 3 cut(s) 1102, 1354, 2812
Bsp1407I TGTACA 1 cut(s) 992
Bsp143I GATC 9 cut(s) 862, 1264, 1658, 1975, 2119, 2605, 2695, 2902, 2973
BspANI GGCC 6 cut(s) 130, 901, 1139, 1352, 2015, 2063
BspCNI CTCAG 3 cut(s) 1402, 1615, 2209
BspDI ATCGAT 1 cut(s) 576
BspHI TCATGA 1 cut(s) 2905
BspLI GGNNCC 8 cut(s) 783, 835, 887, 1352, 1944, 1977, 2100, 2830
BspMAI CTGCAG 2 cut(s) 1900, 2968
BspMI ACCTGC 1 cut(s) 1903
BspPI GGATC 6 cut(s) 1259, 1666, 1970, 1983, 2127, 2690
BspT104I TTCGAA 1 cut(s) 2180
BspT107I GGYRCC 1 cut(s) 1942
BsrBI CCGCTC 1 cut(s) 601
BsrDI GCAATG 1 cut(s) 2592
BsrFI RCCGGY 1 cut(s) 683
BsrGI TGTACA 1 cut(s) 992
BsrI ACTGG 4 cut(s) 570, 1496, 1701, 2755
BssAI RCCGGY 1 cut(s) 683
BssECI CCNNGG 5 cut(s) 36, 613, 893, 902, 3006
BssMI GATC 9 cut(s) 862, 1264, 1658, 1975, 2119, 2605, 2695, 2902, 2973
BssNI GRCGYC 1 cut(s) 1434
BssSI CACGAG 2 cut(s) 411, 506
Bst2BI CACGAG 2 cut(s) 411, 506
Bst2UI CCWGG 5 cut(s) 461, 615, 895, 903, 2096
Bst4CI ACNGT 3 cut(s) 2199, 2539, 3155
Bst6I CTCTTC 9 cut(s) 96, 162, 420, 462, 467, 1219, 1902, 2255, 3006
BstACI GRCGYC 1 cut(s) 1434
BstAPI GCANNNNNTGC 1 cut(s) 1895
BstAUI TGTACA 1 cut(s) 992
BstBAI YACGTR 1 cut(s) 2663
BstBI TTCGAA 1 cut(s) 2180
BstC8I GCNNGC 6 cut(s) 2065, 2153, 2302, 2364, 2472, 2928
BstDEI CTNAG 7 cut(s) 261, 1389, 1623, 2217, 2766, 2813, 2835
BstENI CCTNNNNNAGG 1 cut(s) 2031
BstHHI GCGC 2 cut(s) 538, 963
BstKTI GATC 9 cut(s) 865, 1267, 1661, 1978, 2122, 2608, 2698, 2905, 2976
BstMAI GTCTC 5 cut(s) 164, 1117, 2004, 3003, 3277
BstMBI GATC 9 cut(s) 862, 1264, 1658, 1975, 2119, 2605, 2695, 2902, 2973
BstMWI GCNNNNNNNGC 5 cut(s) 1295, 1895, 2771, 2801, 3128
BstNI CCWGG 5 cut(s) 461, 615, 895, 903, 2096
BstNSI RCATGY 1 cut(s) 1616
BstPAI GACNNNNGTC 1 cut(s) 1190
BstSCI CCNGG 8 cut(s) 35, 459, 613, 893, 901, 2094, 3087, 3143
BstSFI CTRYAG 6 cut(s) 132, 1796, 1834, 1896, 2964, 3261
BstSLI GKGCMC 1 cut(s) 1354
BstV2I GAAGAC 1 cut(s) 3086
BstX2I RGATCY 3 cut(s) 1975, 2605, 2695
BstYI RGATCY 3 cut(s) 1975, 2605, 2695
Bsu15I ATCGAT 1 cut(s) 576
BsuRI GGCC 6 cut(s) 130, 901, 1139, 1352, 2015, 2063
BsuTUI ATCGAT 1 cut(s) 576
BtsI GCAGTG 3 cut(s) 1617, 2925, 3145
BtsIMutI CAGTG 4 cut(s) 1617, 2535, 2925, 3145
BveI ACCTGC 1 cut(s) 1903
Cac8I GCNNGC 6 cut(s) 2065, 2153, 2302, 2364, 2472, 2928
CaiI CAGNNNCTG 3 cut(s) 521, 1895, 1904
CciI TCATGA 1 cut(s) 2905
CfoI GCGC 2 cut(s) 538, 963
Cfr10I RCCGGY 1 cut(s) 683
Cfr13I GGNCC 6 cut(s) 128, 833, 1350, 1351, 3044, 3164
ClaI ATCGAT 1 cut(s) 576
CseI GACGC 2 cut(s) 1442, 3128
Csp6I GTAC 5 cut(s) 993, 2038, 2393, 3156, 3193
CspAI ACCGGT 1 cut(s) 683
CspCI CAANNNNNGTGG 2 cut(s) 2770, 2805
CviQI GTAC 5 cut(s) 993, 2038, 2393, 3156, 3193
DdeI CTNAG 7 cut(s) 261, 1389, 1623, 2217, 2766, 2813, 2835
DpnI GATC 9 cut(s) 864, 1266, 1660, 1977, 2121, 2607, 2697, 2904, 2975
DpnII GATC 9 cut(s) 862, 1264, 1658, 1975, 2119, 2605, 2695, 2902, 2973
DraI TTTAAA 2 cut(s) 1816, 3051
DriI GACNNNNNGTC 1 cut(s) 2995
EaeI YGGCCR 1 cut(s) 2013
Eam1104I CTCTTC 9 cut(s) 96, 162, 420, 462, 467, 1219, 1902, 2255, 3006
Eam1105I GACNNNNNGTC 1 cut(s) 2995
EarI CTCTTC 9 cut(s) 96, 162, 420, 462, 467, 1219, 1902, 2255, 3006
EciI GGCGGA 1 cut(s) 380
Ecl136II GAGCTC 1 cut(s) 2810
Eco24I GRGCYC 2 cut(s) 1354, 2812
Eco47I GGWCC 3 cut(s) 833, 3044, 3164
Eco53kI GAGCTC 1 cut(s) 2810
Eco57I CTGAAG 5 cut(s) 762, 1590, 1926, 2370, 3095
Eco72I CACGTG 1 cut(s) 2663
Eco88I CYCGRG 1 cut(s) 192
EcoICRI GAGCTC 1 cut(s) 2810
EcoNI CCTNNNNNAGG 1 cut(s) 2031
EcoO109I RGGNCCY 1 cut(s) 128
EcoRII CCWGG 5 cut(s) 459, 613, 893, 901, 2094
EcoT22I ATGCAT 1 cut(s) 1516
EcoT38I GRGCYC 2 cut(s) 1354, 2812
FalI AAGNNNNNCTT 6 cut(s) 264, 296, 2838, 2870, 3199, 3231
FaqI GGGAC 3 cut(s) 202, 819, 2148
FauI CCCGC 1 cut(s) 3204
FblI GTMKAC 1 cut(s) 1637
FriOI GRGCYC 2 cut(s) 1354, 2812
FspBI CTAG 7 cut(s) 644, 866, 1221, 1331, 2106, 2111, 3279
GlaI GCGC 2 cut(s) 537, 962
GsaI CCCAGC 1 cut(s) 551
GsuI CTGGAG 3 cut(s) 1445, 1684, 2826
HaeIII GGCC 6 cut(s) 130, 901, 1139, 1352, 2015, 2063
HapII CCGG 4 cut(s) 36, 684, 3088, 3144
HgaI GACGC 2 cut(s) 1442, 3128
HhaI GCGC 2 cut(s) 538, 963
Hin1I GRCGYC 1 cut(s) 1434
Hin6I GCGC 2 cut(s) 536, 961
HinP1I GCGC 2 cut(s) 536, 961
HindIII AAGCTT 3 cut(s) 855, 3129, 3213
HpaII CCGG 4 cut(s) 36, 684, 3088, 3144
Hpy166II GTNNAC 4 cut(s) 278, 620, 641, 1638
Hpy8I GTNNAC 4 cut(s) 278, 620, 641, 1638
HpyCH4III ACNGT 3 cut(s) 2199, 2539, 3155
HpyCH4IV ACGT 2 cut(s) 656, 2662
HpyF10VI GCNNNNNNNGC 5 cut(s) 1295, 1895, 2771, 2801, 3128
HpyF3I CTNAG 7 cut(s) 261, 1389, 1623, 2217, 2766, 2813, 2835
HpySE526I ACGT 2 cut(s) 656, 2662
Hsp92I GRCGYC 1 cut(s) 1434
HspAI GCGC 2 cut(s) 536, 961
Kzo9I GATC 9 cut(s) 862, 1264, 1658, 1975, 2119, 2605, 2695, 2902, 2973
LweI GCATC 7 cut(s) 487, 690, 1240, 1477, 1501, 1891, 1983
MaeI CTAG 7 cut(s) 644, 866, 1221, 1331, 2106, 2111, 3279
MaeII ACGT 2 cut(s) 656, 2662
MaeIII GTNAC 6 cut(s) 565, 1339, 1615, 2328, 2436, 2477
MalI GATC 9 cut(s) 864, 1266, 1660, 1977, 2121, 2607, 2697, 2904, 2975
MbiI CCGCTC 1 cut(s) 601
MboI GATC 9 cut(s) 862, 1264, 1658, 1975, 2119, 2605, 2695, 2902, 2973
MfeI CAATTG 2 cut(s) 1719, 2123
MflI RGATCY 3 cut(s) 1975, 2605, 2695
MhlI GDGCHC 3 cut(s) 1102, 1354, 2812
MlyI GAGTC 9 cut(s) 184, 346, 590, 1736, 2272, 2675, 2870, 2984, 3005
MmeI TCCRAC 4 cut(s) 505, 1355, 1801, 2202
Mph1103I ATGCAT 1 cut(s) 1516
MspA1I CMGCKG 2 cut(s) 551, 1393
MspI CCGG 4 cut(s) 36, 684, 3088, 3144
MspR9I CCNGG 8 cut(s) 37, 461, 615, 895, 903, 2096, 3089, 3145
MunI CAATTG 2 cut(s) 1719, 2123
Mva1269I GAATGC 1 cut(s) 220
MvaI CCWGG 5 cut(s) 461, 615, 895, 903, 2096
MwoI GCNNNNNNNGC 5 cut(s) 1295, 1895, 2771, 2801, 3128
NciI CCSGG 3 cut(s) 37, 3089, 3145
NdeII GATC 9 cut(s) 862, 1264, 1658, 1975, 2119, 2605, 2695, 2902, 2973
NlaIV GGNNCC 8 cut(s) 783, 835, 887, 1352, 1944, 1977, 2100, 2830
NmuCI GTSAC 4 cut(s) 1615, 2328, 2436, 2477
NsiI ATGCAT 1 cut(s) 1516
NspI RCATGY 1 cut(s) 1616
NspV TTCGAA 1 cut(s) 2180
PagI TCATGA 1 cut(s) 2905
PaqCI CACCTGC 1 cut(s) 1903
PciI ACATGT 1 cut(s) 1612
PctI GAATGC 1 cut(s) 220
PflFI GACNNNGTC 1 cut(s) 517
PflMI CCANNNNNTGG 1 cut(s) 791
PinAI ACCGGT 1 cut(s) 683
PleI GAGTC 9 cut(s) 184, 345, 589, 1736, 2271, 2674, 2870, 2984, 3004
PmaCI CACGTG 1 cut(s) 2663
PmlI CACGTG 1 cut(s) 2663
PpsI GAGTC 9 cut(s) 184, 345, 589, 1736, 2271, 2674, 2870, 2984, 3004
Ppu21I YACGTR 1 cut(s) 2663
PscI ACATGT 1 cut(s) 1612
PshAI GACNNNNGTC 1 cut(s) 1190
Psp124BI GAGCTC 1 cut(s) 2812
Psp6I CCWGG 5 cut(s) 459, 613, 893, 901, 2094
PspCI CACGTG 1 cut(s) 2663
PspFI CCCAGC 1 cut(s) 547
PspGI CCWGG 5 cut(s) 459, 613, 893, 901, 2094
PspN4I GGNNCC 8 cut(s) 783, 835, 887, 1352, 1944, 1977, 2100, 2830
PspOMI GGGCCC 1 cut(s) 1350
PspPI GGNCC 6 cut(s) 128, 833, 1350, 1351, 3044, 3164
PstI CTGCAG 2 cut(s) 1900, 2968
PstNI CAGNNNCTG 3 cut(s) 521, 1895, 1904
PsuI RGATCY 3 cut(s) 1975, 2605, 2695
PsyI GACNNNGTC 1 cut(s) 517
PvuII CAGCTG 2 cut(s) 551, 1393
RsaI GTAC 5 cut(s) 994, 2039, 2394, 3157, 3194
RsaNI GTAC 5 cut(s) 993, 2038, 2393, 3156, 3193
SacI GAGCTC 1 cut(s) 2812
Sau3AI GATC 9 cut(s) 862, 1264, 1658, 1975, 2119, 2605, 2695, 2902, 2973
Sau96I GGNCC 6 cut(s) 128, 833, 1350, 1351, 3044, 3164
SchI GAGTC 9 cut(s) 184, 346, 590, 1736, 2272, 2675, 2870, 2984, 3005
ScrFI CCNGG 8 cut(s) 37, 461, 615, 895, 903, 2096, 3089, 3145
SduI GDGCHC 3 cut(s) 1102, 1354, 2812
SfaNI GCATC 7 cut(s) 487, 690, 1240, 1477, 1501, 1891, 1983
SfcI CTRYAG 6 cut(s) 132, 1796, 1834, 1896, 2964, 3261
SfuI TTCGAA 1 cut(s) 2180
SinI GGWCC 3 cut(s) 833, 3044, 3164
SmlI CTYRAG 3 cut(s) 1114, 2855, 3188
SmoI CTYRAG 3 cut(s) 1114, 2855, 3188
SspI AATATT 2 cut(s) 1872, 2142
SspMI CTAG 7 cut(s) 644, 866, 1221, 1331, 2106, 2111, 3279
SstI GAGCTC 1 cut(s) 2812
StyD4I CCNGG 8 cut(s) 35, 459, 613, 893, 901, 2094, 3087, 3143
TaaI ACNGT 3 cut(s) 2199, 2539, 3155
TaiI ACGT 2 cut(s) 659, 2665
TaqI TCGA 5 cut(s) 248, 344, 576, 1248, 2180
TatI WGTACW 2 cut(s) 992, 3155
TauI GCSGC 3 cut(s) 119, 2018, 3112
TscAI CASTG 4 cut(s) 1624, 2542, 2925, 3145
TseFI GTSAC 4 cut(s) 1615, 2328, 2436, 2477
Tsp45I GTSAC 4 cut(s) 1615, 2328, 2436, 2477
TspRI CASTG 4 cut(s) 1624, 2542, 2925, 3145
Tth111I GACNNNGTC 1 cut(s) 517
Van91I CCANNNNNTGG 1 cut(s) 791
VpaK11BI GGWCC 3 cut(s) 833, 3044, 3164
XagI CCTNNNNNAGG 1 cut(s) 2031
XapI RAATTY 3 cut(s) 848, 1076, 2888
XbaI TCTAGA 1 cut(s) 2110
XceI RCATGY 1 cut(s) 1616
XcmI CCANNNNNNNNNTGG 1 cut(s) 2632
XmiI GTMKAC 1 cut(s) 1637
XspI CTAG 7 cut(s) 644, 866, 1221, 1331, 2106, 2111, 3279
Zsp2I ATGCAT 1 cut(s) 1516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.